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<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNP4</accession>
    <name>A0A2S2KNP4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNP4"/>
    <dbReference type="OrthoDB" id="3132at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="233" mass="26518" checksum="584D32D2DD8861CC" modified="2018-07-18" version="1">MLAFLSFSIFGNDLAEAQVDPLSNIVFLQTGQINTDENQFQISNDITVREFFGGKIIRISGQTIEGFPYITYSKVSEEKTDSRGIIFINDQFVNLSFQEKITDSKTITEKNDDLAILVQYTQRVYSEKYAKIDVKIFEKSQNKLNDFYQNYGYKQNTNISVSIINEDDQTAFSSNGITDKNGLYQTEFFIPENFKRQTLTVTIIAENNDSKSSRVLQIFSLGQQSDDGKSSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNR5</accession>
    <name>A0A2S2KNR5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Coenzyme Q-binding protein COQ10 START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_00570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875937.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNR5"/>
    <dbReference type="GeneID" id="76209386"/>
    <dbReference type="OrthoDB" id="10113at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07812">
        <property type="entry name" value="SRPBCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005031">
        <property type="entry name" value="COQ10_START"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03364">
        <property type="entry name" value="Polyketide_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Coenzyme Q-binding protein COQ10 START" evidence="1">
        <location>
            <begin position="10"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="142" mass="16322" checksum="2B556A72F1418F2D" modified="2018-07-18" version="1">MSEFTLERIVNAKREVVFEILSNYENYQKLMPQHFPSIRVRSIRGNVAVVEEHLNLGGEEMVIMSKHVSQEPALHEIFVIGGDAKGSHIKQQFIEHEEGTKIFINVNLKLKGKMKIGQLFGNKKYESSYSKIIDDFLRTAET</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNS9</accession>
    <name>A0A2S2KNS9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP-dependent dethiobiotin synthetase BioD</fullName>
            <ecNumber evidence="8">6.3.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">DTB synthetase</fullName>
            <shortName evidence="8">DTBS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Dethiobiotin synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">bioD</name>
        <name evidence="9" type="ORF">NZNM25_00080</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA, also called 7,8-diammoniononanoate) to form a ureido ring.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(7R,8S)-7,8-diammoniononanoate + CO2 + ATP = (4R,5S)-dethiobiotin + ADP + phosphate + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15805"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:149469"/>
            <dbReference type="ChEBI" id="CHEBI:149473"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the dethiobiotin synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.3" evidence="8"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875892.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNS9"/>
    <dbReference type="GeneID" id="76209334"/>
    <dbReference type="OrthoDB" id="50320at2157"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00161"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004141">
        <property type="term" value="F:dethiobiotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03109">
        <property type="entry name" value="DTBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000292">
        <property type="entry name" value="ATP-dependent dethiobiotin synthetase BioD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00336">
        <property type="entry name" value="BioD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004472">
        <property type="entry name" value="DTB_synth_BioD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00347">
        <property type="entry name" value="bioD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43210">
        <property type="entry name" value="DETHIOBIOTIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43210:SF5">
        <property type="entry name" value="DETHIOBIOTIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13500">
        <property type="entry name" value="AAA_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006755">
        <property type="entry name" value="DTB_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="8">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="114"/>
            <end position="117"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="174"/>
            <end position="175"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="202"/>
            <end position="204"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="227" mass="24984" checksum="BE46259BB148FA47" modified="2018-07-18" version="1">MKSFFITGTDTDVGKTYVTAGLAVTLRKMGIDVGVMKPFAAGVVQKNGFKSEDVEILSNAAGISDPENLVNPQFFPIPASPYTASRMLKIKPKIATVLNSFKKLSQLHELILVEGMGGILTPIKKNYYVSSLIKDMNIPTVIVASSRIGTVNHTLMTCIMCKNYKIPIKGIIINNFEKGYSISELKQDIENLTGVKVLGSIPFIKNMSDSSLNRIFTKNLDMRIFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNW1</accession>
    <name>A0A2S2KNW1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF427 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_00480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875928.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNW1"/>
    <dbReference type="GeneID" id="76209377"/>
    <dbReference type="OrthoDB" id="88627at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.40">
        <property type="entry name" value="Domain of unknown function (DUF427)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007361">
        <property type="entry name" value="DUF427"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038694">
        <property type="entry name" value="DUF427_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34310">
        <property type="entry name" value="DUF427 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_3G02220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34310:SF5">
        <property type="entry name" value="DUF427 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_3G02220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04248">
        <property type="entry name" value="NTP_transf_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF427" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="94" mass="10763" checksum="B7EB3501C420CAFA" modified="2018-07-18" version="1">MQAIWNDVVIAESDNTVVVEGNHYFPFDSIKKEYFQKTDLTTFCGWKGMANYYSVTVNGKTNKDCAWYYEQPNDAAMKIKGMVAFWNGVKVVNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNX9</accession>
    <name>A0A2S2KNX9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cytochrome C biogenesis protein CcdA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ccdA</name>
        <name evidence="8" type="ORF">NZNM25_00120</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DsbD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875896.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNX9"/>
    <dbReference type="GeneID" id="76209338"/>
    <dbReference type="OrthoDB" id="115386at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003834">
        <property type="entry name" value="Cyt_c_assmbl_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051790">
        <property type="entry name" value="Cytochrome_c-biogenesis_DsbD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31272:SF9">
        <property type="entry name" value="BLL1027 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31272">
        <property type="entry name" value="CYTOCHROME C-TYPE BIOGENESIS PROTEIN HI_1454-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02683">
        <property type="entry name" value="DsbD_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome C biogenesis protein transmembrane" evidence="7">
        <location>
            <begin position="6"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="244" mass="26212" checksum="BA62EE9BA4327CA4" modified="2018-07-18" version="1">MSEINLAIAALAGLGSFVAPCILPMIPAFLAYISGTTLTELNQAGNAKVFTINRTNIVLNSVFFVLGFSVVFSILGVIINSVLGTAAEEFVGVLNQVGGIIIIGFGIFLLLSTKINKLNIEKKFFPKRSKASYPMSFVFGLAFAAGWTPCVGPILGTILTLAATTPSVAFNLLLVYSLGLGIPFILIGVFYSRATRIIRSMSKHLKYYNLVLGGFIMILGILVFTNQLAYIANFPLLNELLFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP62</accession>
    <name>A0A2S2KP62_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">D-lactate dehydrogenase (cytochrome)</fullName>
            <ecNumber evidence="7">1.1.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_01020</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.2.4" evidence="7"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP62"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008720">
        <property type="term" value="F:D-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903457">
        <property type="term" value="P:lactate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.45.10:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748:SF111">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="8">
        <location>
            <begin position="6"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="455" mass="51296" checksum="7BD82EFABD53BF6F" modified="2018-07-18" version="1">MDSSSYQIIPSVVIVPKDEKDIMRAIKIAKKFKSSVTVRGAGTGLVGSALNNGIILDMKNFNSIKITKNYAIVGPGVIKGELDKKLEIHDKFFPPNPSIGSFCTVGGMIGNNSSGSKSLKYGSVIDNVAEIIFVDGNGKRISLPKDKKVSKKILEFSKKIKIEKFPNVTKNSSGYRIDKIRSITDAHKLIIGSEGTLGIALSIKLKIRDRQEKKILFIIEFKSIIDASRNCLEINKTMPSAIEFVDRTTLNQINFKFSQDTQCLLLVEYDEDIDRSEKKLKSIITGKIVKKLKKDSEINEWWRYRDLSLHYSLKSIKKEKRIPHVIEDAAVPLENLPEIFKILKKINKKFKTKTIVYGHAGNGNIHVRLISDRSEFTAIKKIAILYFDEIIKLGGTITAEHGDGLARSEFIKKQYGKDNYKIFKEIKQFFDPKNILNPGKIITSKSTIIDNLEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP77</accession>
    <name>A0A2S2KP77_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoserine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">O-phosphoserine phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">NZNM25_02300</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAD-like hydrolase superfamily. SerB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.3" evidence="4"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876260.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP77"/>
    <dbReference type="GeneID" id="76209564"/>
    <dbReference type="OrthoDB" id="10041at2157"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00198"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036424">
        <property type="term" value="F:L-phosphoserine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000390">
        <property type="entry name" value="Phosphoserine phosphatase (SerB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050582">
        <property type="entry name" value="HAD-like_SerB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004469">
        <property type="entry name" value="PSP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01488">
        <property type="entry name" value="HAD-SF-IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00338">
        <property type="entry name" value="serB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344:SF2">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0718">Serine biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH33439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="216" mass="24221" checksum="F1703FB634C837E9" modified="2018-07-18" version="1">MLAIFDVEGVLYDEEYLPLLAEKLHKEDEIWEITKQGIQGKINWEEGLRTRVGALKGLDQKMCQEVADALPIMTGAKELCRVLKAAGWKLMAVSGGFTLMMDRLQKELGLDYVFSNELLFKDGKLDGVIINVDSDKSKSAKIKIEEWGEKREDIVCVVDGANDVKLFDICGLGIAYRAQDIVKDLATTTLEEKDLSKVLDIINKHYKIQLETVSPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP78</accession>
    <name>A0A2S2KP78_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thiamine thiazole synthase</fullName>
            <ecNumber evidence="6">2.4.2.59</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">thi4</name>
        <name evidence="7" type="ORF">NZNM25_01850</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>hydrogen sulfide + glycine + NAD(+) = ADP-5-ethyl-4-methylthiazole-2-carboxylate + nicotinamide + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:139151"/>
            <dbReference type="EC" id="2.4.2.59"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homooctamer; tetramer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the THI4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.59" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP78"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00304">
        <property type="entry name" value="Thi4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002922">
        <property type="entry name" value="Thi4_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022828">
        <property type="entry name" value="Thi4_prok"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00292">
        <property type="entry name" value="sulfide-dependent adenosine diphosphate thiazole synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43422">
        <property type="entry name" value="THIAMINE THIAZOLE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43422:SF3">
        <property type="entry name" value="THIAMINE THIAZOLE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01946">
        <property type="entry name" value="Thi4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <begin position="77"/>
            <end position="78"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>glycine</name>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="280" mass="30523" checksum="213C85030EEFD10D" modified="2018-07-18" version="1">MLRKRNHDMQEATVAEQSNKIFTDVREVEITRAIANEFHDVLIDRADSDVIIIGAGPAGLTASRELSNMGFKVLVIEQNNYLGGGYWLGGYMMNPVTVREPAQKFWDELGIPYKKVADGLYLTPGPHAVSKLIAAACDAGVKFLQLTKFDDLVLKNGRVTGIVVNWMPVSALPRNITCVDPVALEAKIIIDASGHDSVAVKRLVDRGLAKWKGMEPMHVNDGEEHVVHKTGEVYPGLVAAGMSVTETHGLARMGPTFGSMLYSGKKAAEITAAKIKELER</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP97</accession>
    <name>A0A2S2KP97_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chemotaxis methyl-accepting receptor HlyB-like 4HB MCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_02050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146195964.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP97"/>
    <dbReference type="GeneID" id="76209539"/>
    <dbReference type="OrthoDB" id="11248at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="170" mass="19279" checksum="4DDCB9D1632EB2C9" modified="2018-07-18" version="1">MNMQKRNAGAIIFSILVIAGFSLSVNFYQESVHPLELAISHLKEITASSDPKTIREHISAMNYHVDKIWDKLPESKNPVWIYPTESTNFARIQSDVDRIVVSLDKISAVPRDSSAYHTGMMDIKERTRHIQENISDAMPYMYGTLSMAFFNSILLVGLMGLIERITGKPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPA6</accession>
    <name>A0A2S2KPA6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxypeptidase regulatory-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875990.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPA6"/>
    <dbReference type="GeneID" id="76209445"/>
    <dbReference type="OrthoDB" id="11442at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010916">
        <property type="entry name" value="TonB_box_CS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00430">
        <property type="entry name" value="TONB_DEPENDENT_REC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="34925" checksum="0F3073607A99EEC2" modified="2018-07-18" version="1">MYENRLLGIGLISFLMLFSIISTSYAELWELVIEVNVEKGAIYSGDSVIVTGKVVDQAYKPIRGAEVFIKAGSYTTKAFTDPEGVFKGEIKDFERIPGTYIVNVIGSWYGMTGLSSTEFQVKGDISPVSELQEKLSTDEAIKYLSSDESNFEKNPIGQTLFKYYHKLLEELILEQKIAQKPSEDKIFMEHQRLIAENLRNQAIDEFKPGAGTYDGIQYNDYINNLNPKIKELVINQLNFTKNNFAEAQKIRDDILANGGTYEDARQAYLDRISIPKETLEEFNKEKIDQELEESLENNTTDENSENQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPB0</accession>
    <name>A0A2S2KPB0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA polymerase sigma factor 70 region 4 type 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876155.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPB0"/>
    <dbReference type="GeneID" id="76209638"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="77" mass="8671" checksum="07756A05938D0471" modified="2018-07-18" version="1">MNDKALESIDNRLKIISKLLALDVVKGRQFQEQVKILHEMGMQPSEIADCLGKTANNVRVAVHGIKKKSTQKEVKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPB6</accession>
    <name>A0A2S2KPB6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_01260</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875999.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPB6"/>
    <dbReference type="GeneID" id="76209455"/>
    <dbReference type="OrthoDB" id="4612at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08072">
        <property type="entry name" value="MPN_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028090">
        <property type="entry name" value="JAB_dom_prok"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14464">
        <property type="entry name" value="Prok-JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="JAB" evidence="6">
        <location>
            <begin position="16"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="132" mass="14706" checksum="1F36B9B284BD467C" modified="2018-07-18" version="1">MIFKKKEFKRTVLIQKEVLDSILSFCQMKHPNEGILILKGKSKNGEITISGLVIPPFSETGPTFAGFPHSFLPFDMSYVGIVHSHPSGSAEPSVTDLHNFFGLVSMIVQSPYDENSIFAWDRDGNQIPHSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPC3</accession>
    <name>A0A2S2KPC3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GYD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876174.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPC3"/>
    <dbReference type="GeneID" id="76209662"/>
    <dbReference type="OrthoDB" id="7321at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="11264" checksum="EA5B22F2463C8572" modified="2018-07-18" version="1">MLFGVFAVHSPESCPMNNESSRKMFVQVQKKIEENSEKFKISKIIGFYMSVLEHEWIIILDADNSHDIEKLCIEVGISSVSTVKIVPINAYSDAIKKMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPG4</accession>
    <name>A0A2S2KPG4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_01490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876018.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPG4"/>
    <dbReference type="GeneID" id="76209479"/>
    <dbReference type="OrthoDB" id="385995at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="170" mass="19616" checksum="2390500786703694" modified="2018-07-18" version="1">MTKFSAVIEINKTHEKETLEKISSLYSKSTNKRIDSKDSKIRYVLKVPTSDSKEILENNLQGINNLEIKMLESEPNVLVRLPRKISTWIMVGFFVVWIGISYVAFFISDNILDNLSNSQILVIQVSIALVISIWLFFYDKRTQEEIVDVLAKLDLRTSAMDENIKDLDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPH4</accession>
    <name>A0A2S2KPH4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">3-dehydroquinate dehydratase</fullName>
            <shortName evidence="6">3-dehydroquinase</shortName>
            <ecNumber evidence="6">4.2.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Type I DHQase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Type I dehydroquinase</fullName>
            <shortName evidence="6">DHQ1</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">aroD</name>
        <name evidence="7" type="ORF">NZNM25_03470</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the third step of the chorismate pathway, which leads to the biosynthesis of aromatic amino acids. Catalyzes the cis-dehydration of 3-dehydroquinate (DHQ) and introduces the first double bond of the aromatic ring to yield 3-dehydroshikimate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>3-dehydroquinate = 3-dehydroshikimate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="EC" id="4.2.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the type-I 3-dehydroquinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.10" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876195.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPH4"/>
    <dbReference type="GeneID" id="76209687"/>
    <dbReference type="OrthoDB" id="34329at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00086"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003855">
        <property type="term" value="F:3-dehydroquinate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046279">
        <property type="term" value="P:3,4-dihydroxybenzoate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00502">
        <property type="entry name" value="DHQase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000290">
        <property type="entry name" value="3-dehydroquinate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00214">
        <property type="entry name" value="AroD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001381">
        <property type="entry name" value="DHquinase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050146">
        <property type="entry name" value="Type-I_3-dehydroquinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01093">
        <property type="entry name" value="aroD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43699">
        <property type="entry name" value="3-DEHYDROQUINATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43699:SF1">
        <property type="entry name" value="3-DEHYDROQUINATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01487">
        <property type="entry name" value="DHquinase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 6" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="4 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="6">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="6">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="32"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="222" mass="25317" checksum="77F268630906496E" modified="2018-07-18" version="1">MKYKTCVSIAETTPNKVKQVLKNALKKSDFVEVRFDFLKMEQIPQTLELIKKDLNKIVCTLRPKTEGGKFPGNEKERMSIIKLIAEYNPFLLDVEFNMLKKNPQLTEYLKSTKTELLVSWHDFKKTPKATELKKKITQMSKFSNNIKIVSTAKTTDDATRMLELYSKKGKNNLISFAMGDMGRISRILCLYLGSPYTYVSLGKAVAPGQFSVDEVKKIASLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPI5</accession>
    <name>A0A2S2KPI5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01960</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876063.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPI5"/>
    <dbReference type="GeneID" id="76209528"/>
    <dbReference type="OrthoDB" id="383958at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="284" mass="28754" checksum="6BA03969697A4A2B" modified="2018-07-18" version="1">MTHKTKSILFVFAFAAIMLAWPMTSQNAGADGDATITAECGFTTPSDINLGTIAIGVDSTEVLTTIPMTGTVTGTLELQATDWTGVGTSATGSITLVGVTDGETVEVNSNTYTAETTLPLDAGEFDVTGTDAAAATSLASVINSEDAAITATVPTSTNVILLSVDALGTAGNAVTLTESVSNTGTTVFPTDGSFDGAEASGVVHMQAEAIKYAITTDGTTNAGTDYTTGKAATGVDSQVFVLTTNTDPEESVRLYLQASGDGTLENLPYSGALESTFAFGSTCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPP6</accession>
    <name>A0A2S2KPP6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_04410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829051.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPP6"/>
    <dbReference type="GeneID" id="76209790"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="99" mass="11196" checksum="B8B61894CF9D7AA4" modified="2018-07-18" version="1">MGLSSFIEKYREKENEIYLTWSDLAIVVFFLIQTVMPFPYGLIFGFVFAVAISFALFSLFTGDGNPAFTSGIQLDDLDHVDDLDGKNVKIYDKVIDKVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPQ1</accession>
    <name>A0A2S2KPQ1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SHOCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876178.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPQ1"/>
    <dbReference type="GeneID" id="76209666"/>
    <dbReference type="OrthoDB" id="3275at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="175" mass="20147" checksum="94F915A244C86560" modified="2018-07-18" version="1">MEIKSEKSIKEFLKTLPDDDIIKYYLDVEYSPFPVLVIEEYTRRFKRKTKDEILKDLKTQARFAHKKTREFGKIAKKHKFVDDVTKQKSEEIFKQAKKKGFEISEKLSYKGGILGSKLKKGTTSGIKSGINAGKNIKSSSVDELELLEKLGDLQKAGIITKKEFLEKKKKILSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPR5</accession>
    <name>A0A2S2KPR5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9 10">Prefoldin subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">GimC subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pfdB</name>
        <name evidence="13" type="ORF">NZNM25_04610</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">Molecular chaperone capable of stabilizing a range of proteins. Seems to fulfill an ATP-independent, HSP70-like function in archaeal de novo protein folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Heterohexamer of two alpha and four beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876331.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPR5"/>
    <dbReference type="GeneID" id="76209813"/>
    <dbReference type="OrthoDB" id="11287at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23162">
        <property type="entry name" value="Prefoldin_beta_GimC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000013">
        <property type="entry name" value="Prefoldin subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00307">
        <property type="entry name" value="PfdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012713">
        <property type="entry name" value="PfdB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02338">
        <property type="entry name" value="gimC_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21431">
        <property type="entry name" value="PREFOLDIN SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21431:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="104"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="17"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="NCBIfam" id="TIGR02338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH33670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="129" mass="14653" checksum="11FBD255737C2A09" modified="2018-07-18" version="1">MSSPQMPPWLQEQIMKLQQSQQSLQSVMTQKQHLEMEKAETAKALDELKKVADGDAVFKQAGTVLIKAKKQELIDELEERAEMAKTRVTVLDKQEIRLKESLKEQETKITEMMKGGSTSTPPAEDNPRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPY7</accession>
    <name>A0A2S2KPY7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein uL15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl15</name>
        <name evidence="8" type="ORF">NZNM25_05140</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876376.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPY7"/>
    <dbReference type="GeneID" id="76209865"/>
    <dbReference type="OrthoDB" id="9418at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.990.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_uL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027386">
        <property type="entry name" value="Rbsml_uL15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_uL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="7">
        <location>
            <begin position="73"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="141" mass="15497" checksum="185C5E546A641EB8" modified="2018-07-18" version="1">MATRLRKTRRLRGGRHMGWGQVGQHRASGHKGGLGVTGMMKHHWSSTLKYDPDHYGHDSPKTPHPNIAKTWTSVRDLDDLFLKFGKEEGGKKIVDLESAGYEKLLGGGKLTNAYSVKVKQFTATAEEKLKAVGGEVLSNNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPY9</accession>
    <name>A0A2S2KPY9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Translation initiation factor 1A</fullName>
            <shortName evidence="2">aIF-1A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">eif1a</name>
        <name evidence="5" type="ORF">NZNM25_03720</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPY9"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05793">
        <property type="entry name" value="S1_IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00216">
        <property type="entry name" value="aIF_1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001253">
        <property type="entry name" value="TIF_eIF-1A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003084">
        <property type="entry name" value="PRK04012.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003085">
        <property type="entry name" value="PRK04012.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21668">
        <property type="entry name" value="EIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00652">
        <property type="entry name" value="eIF1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2 3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1-like" evidence="4">
        <location>
            <begin position="35"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="125" mass="14735" checksum="681FF5CC08501B34" modified="2018-07-18" version="1">MFCPVIVTENPTSYMLKLLMYGMGKRQVKNESALKEIRLPEEGEQFGRVMKMLGGENVMVKCADNLIRRGRIRGKLKRRVWIRDNDIVIIAPWDFNDAERGDIVWRFTLPQVEWLKDNNHIPKDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ13</accession>
    <name>A0A2S2KQ13_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Leucine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Leucyl-tRNA synthetase</fullName>
            <shortName evidence="8">LeuRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 12" type="primary">leuS</name>
        <name evidence="12" type="ORF">NZNM25_05530</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="8"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876411.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ13"/>
    <dbReference type="GeneID" id="76209903"/>
    <dbReference type="OrthoDB" id="23906at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07959">
        <property type="entry name" value="Anticodon_Ia_Leu_AEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.20">
        <property type="entry name" value="Class I aminoacyl-tRNA synthetases (RS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.720">
        <property type="entry name" value="leucyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00049_A">
        <property type="entry name" value="Leu_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020791">
        <property type="entry name" value="Leu-tRNA-lgase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004493">
        <property type="entry name" value="Leu-tRNA-synth_Ia_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00395">
        <property type="entry name" value="leuS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008957">
        <property type="entry name" value="PRK12300.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794:SF1">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="10">
        <location>
            <begin position="12"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="11">
        <location>
            <begin position="694"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="665"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="621"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="624"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH33762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="955" mass="109648" checksum="2756E23FBEF62EC2" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ19</accession>
    <name>A0A2S2KQ19_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">3-dehydroquinate synthase II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_03460</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876194.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ19"/>
    <dbReference type="GeneID" id="76209686"/>
    <dbReference type="OrthoDB" id="10265at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003856">
        <property type="term" value="F:3-dehydroquinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002812">
        <property type="entry name" value="DHQS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030960">
        <property type="entry name" value="DHQS/DOIS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056179">
        <property type="entry name" value="DHQS_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33563">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33563:SF1">
        <property type="entry name" value="3-DEHYDROQUINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01959">
        <property type="entry name" value="DHQS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26558">
        <property type="entry name" value="DHQS_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006655">
        <property type="entry name" value="DHQ_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-dehydroquinate synthase N-terminal" evidence="3">
        <location>
            <begin position="45"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="3-dehydroquinate synthase C-terminal" evidence="4">
        <location>
            <begin position="177"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF26558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="353" mass="39310" checksum="7C49468399044E84" modified="2018-07-18" version="1">MSKNRELIISPKVSQTQLAKFLPQLESEGIKMLYIDPKKIGNKKTKIQTVYPSPTSNYVVLEKEEVVKPKGKKIGMKFQVLSNSDIEHILTVAKKGLDFVIVEVKDWKIIPLENIIAKLHKIHTKIFAIAKTPEEVRKMFSILEVGVDGVIFNTDSINEVREAMLYLGTRSFEMKPAKIIEIKEVGDGERVCVDTASMLHKGEGMLIGSRSNFLFLVHNESVGSSFTSPRPFRVNAGAVHCYTLSPDGTTNYLSEIETGSEVLILNSKGNARRATVGRSKIERRPMLMIKASIEGEVGGIIAQDAETIRFVKPNGQLVSVTHLKKGDMVMVHSKPATGRHFGMEVSDEYILEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ24</accession>
    <name>A0A2S2KQ24_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_05400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876402.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ24"/>
    <dbReference type="GeneID" id="76209892"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="82" mass="9013" checksum="CC08DC8EC6198F15" modified="2018-07-18" version="1">MTNNKGKKKLFPFGILLTAIGVGLIIISPNMVTETEINNKNNTDTSIIDEESTQQKNPVWDFGLALSIIGIGSMIAGYYRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ58</accession>
    <name>A0A2S2KQ58_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein eS8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">30S ribosomal protein S8e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_03790</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876224.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ58"/>
    <dbReference type="GeneID" id="76209721"/>
    <dbReference type="OrthoDB" id="372305at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.310:FF:000002">
        <property type="entry name" value="30S ribosomal protein S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001047">
        <property type="entry name" value="Ribosomal_eS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022309">
        <property type="entry name" value="Ribosomal_Se8/biogenesis_NSA2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00307">
        <property type="entry name" value="eS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10394">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01201">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 7" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="127" mass="14284" checksum="301A7B601F67537B" modified="2018-07-18" version="1">MRKSVENLATSKITGGRRHPLRIRRKYEIDRYPNEPINAAQISITRRVRGNNRKTALKSIDFVNLATGDAKVKKTKIIKVLDNTTNNDYKRRGIITKGAILETQEGKCKVVSKPGQTGIVNAILLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQA4</accession>
    <name>A0A2S2KQA4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NADH dehydrogenase subunit D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_06340</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the complex I 49 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQA4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="entry name" value="Cytochrome-c3 Hydrogenase, chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014029">
        <property type="entry name" value="NADH_UbQ_OxRdtase_49kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022885">
        <property type="entry name" value="NDH1_su_D/H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993:SF10">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 49 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="HydB/Nqo4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00535">
        <property type="entry name" value="COMPLEX1_49K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-1278">Translocase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="NADH-quinone oxidoreductase subunit D" evidence="7">
        <location>
            <begin position="134"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="NADH-quinone oxidoreductase subunit D" evidence="7">
        <location>
            <begin position="311"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="380" mass="43306" checksum="695A03CF5552AFBA" modified="2018-07-18" version="1">MNMTTELPPGLALQKVDERIMTLNVGPQHPGSGHMRIIVQIDGDYIVACDPDPGYVHRGEEKMAEYRNYITNIPHLERPVIHDSCNILYPYVLGVEEIVGIEVPERAKYVRVIASELNRCIYTMYWLAIYGIFLGHSTMFMWPAGDRELLIDLMEKMTGARVTHAHFVPGGVRNDLPPNFEDVCLRQVNYFEKRIKEYAAVFYDNPILISRTRDTGILSRQDAIRLGTTGSVLRASGVDYDLRLKEPYDVYDELDVHTNVMKEGDSYARSRVPWLDMMESCNIIRQALQKMPKSGSVRTKLKPNPKGKGLNSVYKRVESGRGSLGCYIVSDGKTEPYRLKMSVGSFRNLIALPYLLKGEKLGNMPSVYWSLNYWPVEADR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQA6</accession>
    <name>A0A2S2KQA6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH arsR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_04260</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876303.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQA6"/>
    <dbReference type="GeneID" id="76209774"/>
    <dbReference type="OrthoDB" id="11368at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="HTH arsR-type" evidence="2">
        <location>
            <begin position="38"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="263" mass="29428" checksum="3AEACB6374BA011C" modified="2018-07-18" version="1">MSSDETADDGFTEKIKILATDDKKIKLFGELFTNDSSREILQLLFNDELTANQIAQKTQISLQLVKYHLNKLQDLGVVKISKIEKNSKSQDMKIYSATKFSIVIVPPTLSEKTKESKLLVRSFRHIYKVVGFGIATGLSGLLSLFQLQTKQIPMNEISSKKTAEFSAKNTASFRVDESSESVSAPAPMTAESEPVMEFAKSVEPQDFAEENLDIAETVIDSGVVSSYDLFLPFVIMTVVLGGLTVYYLYKYLKKSKQFMQNPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQB7</accession>
    <name>A0A2S2KQB7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">NAD(P)H-hydrate epimerase</fullName>
            <ecNumber evidence="3 10">5.1.99.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">NAD(P)HX epimerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">nnrE</name>
        <name evidence="12" type="ORF">NZNM25_05950</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>(6R)-NADHX = (6S)-NADHX</text>
            <dbReference type="Rhea" id="RHEA:32215"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:64075"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 10">
            <text>(6R)-NADPHX = (6S)-NADPHX</text>
            <dbReference type="Rhea" id="RHEA:32227"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:64077"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="10">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.99.6" evidence="3 10"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876446.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQB7"/>
    <dbReference type="GeneID" id="76209947"/>
    <dbReference type="OrthoDB" id="15148at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052856">
        <property type="term" value="F:NAD(P)HX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="entry name" value="YjeF N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01966">
        <property type="entry name" value="NADHX_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004443">
        <property type="entry name" value="YjeF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036652">
        <property type="entry name" value="YjeF_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032976">
        <property type="entry name" value="YJEFN_prot_NAXE-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00197">
        <property type="entry name" value="yjeF_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13232">
        <property type="entry name" value="NAD(P)H-HYDRATE EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13232:SF10">
        <property type="entry name" value="NAD(P)H-HYDRATE EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03853">
        <property type="entry name" value="YjeF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64153">
        <property type="entry name" value="YjeF N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51385">
        <property type="entry name" value="YJEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 10" id="KW-0520">NAD</keyword>
    <keyword evidence="6 10" id="KW-0521">NADP</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0630">Potassium</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjeF N-terminal" evidence="11">
        <location>
            <begin position="8"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="57"/>
            <end position="61"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="128"/>
            <end position="134"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH33804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="215" mass="23612" checksum="9B836C0C7A5C3428" modified="2018-07-18" version="1">MEITVDQMYQIENKGHDMGFLKKFMMENAGAASVRRLVDKLGNIESKNIVIFVGLGNNGGDGLVMARHLAGYGAKVTVVLLGTFEKIKTEESNWNWSILEKMTSVKLMTGNSFNFHFTPDVIVDAILGTGISGEIKEPYASAINFINQTDCFKFAVDVPSGLDPQTGETANICTKCDMTVTFHKMKQGIPKRKDLTGELFVEKIGIPPEAEEGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQC1</accession>
    <name>A0A2S2KQC1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Adenylate kinase</fullName>
            <shortName evidence="12">AK</shortName>
            <ecNumber evidence="4 12">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">adkA</name>
        <name evidence="13" type="ORF">NZNM25_05120</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the archaeal adenylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="4 12"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQC1"/>
    <dbReference type="OrthoDB" id="26198at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00234">
        <property type="entry name" value="Adenylate_kinase_AdkA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023477">
        <property type="entry name" value="Adenylate_kinase_AdkA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003122">
        <property type="entry name" value="PRK04040.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13207">
        <property type="entry name" value="AAA_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 12 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="11"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH33721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="192" mass="21558" checksum="65EA46ECD6EE733D" modified="2018-07-18" version="1">MEGNRRIILVGIPGVGKTTILSKMVQILKDHDKTVHVVSFGTLMFEVAKENGLKDRDELRHLPVEKQQQLQKIAAEKIAAYNEQVVIVDTHAFINSPEGYYPGLPEHVLKIIKPTNFVSVSAKPEEIYNRRMKDETRNRDKITLPNINKELDIQSAMISACTVLTGSPVKHILNREGKIDEAIDKIINAIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQG8</accession>
    <name>A0A2S2KQG8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04890</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQG8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="85" mass="9331" checksum="640F009D2D44084E" modified="2018-07-18" version="1">MEEGKQCVNPPEFIVSIVADEDEYMVGVTCNKHKEIVSGKIGILQNEGKMREGKILFSPVKAVGTDCIHGDSSDFVQIDMKSSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQL4</accession>
    <name>A0A2S2KQL4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LLM class flavin-dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_07440</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876605.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQL4"/>
    <dbReference type="GeneID" id="76208932"/>
    <dbReference type="OrthoDB" id="7684at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01097">
        <property type="entry name" value="Tetrahydromethanopterin_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="entry name" value="Luciferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050564">
        <property type="entry name" value="F420-G6PD/mer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43244">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43244:SF1">
        <property type="entry name" value="5,10-METHYLENETETRAHYDROMETHANOPTERIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="Bacterial luciferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Luciferase-like" evidence="2">
        <location>
            <begin position="26"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="366" mass="41325" checksum="65CAA60778D6C86C" modified="2018-07-18" version="1">MASKKLKFGIQNGLNVARAGYTEDQILTACMLADKTGYDSIFYMDHTNVPQWKNATVLDPWVMLSAIAAVTNNVELGTCVTDAIRRHPSNIALAAITLDRVSKGRAILGIGAGEAQNLKEFCIPFEKPVSKWEEQIEVIHTLYNSTPDNIVDYTGKYYQLEGACLQAPPIRKPRPPTYMASGGKRTLELTGKLGDGWLPIGYTPELFEDHKKQIESSMDKHNRTQEEKDNFQMALDIDVYFSEDAEESWAKMKEAVKVSLFKPEVLRVHGLKEIEGFDFVKYFTEYSMSDQSWIVKMREAATKIPDKVARSSTAVGTPEDIIPTFERFMEAGVNHFVIRFWGKNYFGSIDKFASHVMPALREKAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQP3</accession>
    <name>A0A2S2KQP3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_07250</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876588.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQP3"/>
    <dbReference type="GeneID" id="76208950"/>
    <dbReference type="OrthoDB" id="2830at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032993">
        <property type="term" value="C:protein-DNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00156">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="REGULATOR OF RPOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111:SF1">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ORR33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="2"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="165" mass="18957" checksum="8AF151E73042B86E" modified="2018-07-18" version="1">MNVLIVEDEIDLLEEYKIFLESNNHTVTLEMNGESGLKTYFANFDPNSESSPYDVVILDYQLSGKNGMQVASEILAKCPKQRILFASAYVEDTLKESIKTLKQIVELLQKPFGLQKLLNVIEDKTTYEELEKLNVDIKSLKELEPTHAQISTYLDIMRKLHDKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQS6</accession>
    <name>A0A2S2KQS6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876658.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQS6"/>
    <dbReference type="GeneID" id="76208871"/>
    <dbReference type="OrthoDB" id="11066at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="282" mass="32974" checksum="90575148B50264BE" modified="2018-07-18" version="1">MPLDDPTFGNLQNYKLYLRPNAHAHYGHPDQKKSSLSKHQQNVQRLLKVMSVNESSTTWDLAKISIPDDITKLREREKIYRRLLVGRKDKGKHSDGILNLGLAIKDGKSLKTGIADKYRLSLYGILYCIDVLDLSNNEIDKIAKKYSKVLPKVFGKWDYLKTKVGERVYGIKLLANGLLADNPQIQVKSEIPFYELMSYVHLKYQRNFENISEENLAEQISYWFYVNLLYRPMGNNNDTGIESLNPIFQDDPELKKWFLIFFRDSIKYYHERYAVLKKSKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQZ8</accession>
    <name>A0A2S2KQZ8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nicotinate-nucleotide pyrophosphorylase [carboxylating]</fullName>
            <ecNumber evidence="9">2.4.2.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Quinolinate phosphoribosyltransferase [decarboxylating]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_08770</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Involved in the catabolism of quinolinic acid (QA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>nicotinate beta-D-ribonucleotide + CO2 + diphosphate = quinolinate + 5-phospho-alpha-D-ribose 1-diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12733"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 9">Hexamer formed by 3 homodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the NadC/ModD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.19" evidence="9"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876715.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQZ8"/>
    <dbReference type="GeneID" id="76208793"/>
    <dbReference type="OrthoDB" id="115072at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00331"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034213">
        <property type="term" value="P:quinolinate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01572">
        <property type="entry name" value="QPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.20:FF:000001">
        <property type="entry name" value="Nicotinate-nucleotide diphosphorylase (Carboxylating)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000030">
        <property type="entry name" value="Nicotinate-nucleotide pyrophosphorylase, carboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="entry name" value="Quinolinate phosphoribosyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004393">
        <property type="entry name" value="NadC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027277">
        <property type="entry name" value="NadC/ModD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00078">
        <property type="entry name" value="nadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE [CARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179:SF3">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE [CARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006250">
        <property type="entry name" value="NadC_ModD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54675">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 9" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase N-terminal" evidence="11">
        <location>
            <begin position="22"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase C-terminal" evidence="10">
        <location>
            <begin position="105"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR006250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH34086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="272" mass="29800" checksum="957B6EA489CDF2CA" modified="2018-07-18" version="1">MLSFNSKKQLSQFLAEDIGTGDITSSLLSKKKITAKIISRENAVIGGASHAKEIFRLKGCNAKILRKDGSKIKPNQAIMIISGDAGKILACERTVLNLITRMSGIATQTNDLVKKIPKNVKLYATRKTAPGLRYFDKEAVEIGGGKKHRLRLDEMVMIKDNHIAIEGSLESLIKKTKKKYKKFEVEVENTFDAILAAKEGAAIIMLDNFSPVQIKKTVQELKNQKLRNKVMLEASGGINSKNIAKYGKTGVDIISVGSITNSVKGIDVSLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR10</accession>
    <name>A0A2S2KR10_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Serpin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_08870</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the serpin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146195987.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR10"/>
    <dbReference type="GeneID" id="76208783"/>
    <dbReference type="OrthoDB" id="371710at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF211">
        <property type="entry name" value="GH10112P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serpin" evidence="2">
        <location>
            <begin position="75"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="432" mass="49616" checksum="608D6EDE10587F69" modified="2018-07-18" version="1">MKTKFLILLIVIVSASITVILAPTVLYSLDNTDDERKFLEENDVSNSNSMFDGLLQDSPINLSDHASLNSNEFGFKFYKHLSTSDENLFFSPISIDIVFAIIYEAAQGETRENMQQLFSFEQDSEKRRLSYKNTIKNLNQANPDFELNVKNGIWVSDMYELTPEYVKIVTNDYAASSQNVDFVFNHGVQTINQWIKENTNGKIEKIFEDNSTGPLTLMAATNTVYFNGLWEKQFPPGMTYERKFFVTADNVQMIDMMKIKNHHFNYYEDDAVKIVELPYKGNRASMYLLQSFEMHQLKQLEDNLTANYFNELKSKLSEKMVSVIIPKFSMEMDYDLREILTEMGMIHPFSRELADLSGMADYPNIFIDEAVHKTAIDLHELGTEAAAATGAMAELQSGSPYTFYGNHPFVYIIQDHETDQILFMGRFVNPLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR15</accession>
    <name>A0A2S2KR15_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR15"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="148" mass="16672" checksum="DD9870FD38754C87" modified="2018-07-18" version="1">MPLSDAHASCDVFFPIIHNVRAYDEASVVFFGTVTDAHVQSPPVSDPHNSEIIRPSPEYTSFTVHYVLKGELDENSVMTQPHSSVGYRGFVEGETYFVYAYGPQNEVNICTAPTMSLHGLFTFIFHVVFFLIPLGIVITVIIFWKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR18</accession>
    <name>A0A2S2KR18_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876702.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR18"/>
    <dbReference type="GeneID" id="76208816"/>
    <dbReference type="OrthoDB" id="2502at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050697">
        <property type="entry name" value="Adenylyl/Guanylyl_Cyclase_3/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43081:SF1">
        <property type="entry name" value="ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43081">
        <property type="entry name" value="ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="1">
        <location>
            <begin position="82"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="271" mass="30153" checksum="A952A21519DB7F75" modified="2018-07-18" version="1">MENNAKDIAKIGIKNLLSPKTPSIVDMLLGRGTEKVVDSETLVEEIQDRVHKSLNQEFLYSAVTEESEMFLRERVLQSMNMAVLFVDLVGSTSMILNLPKEKLATIFTTFAQEMAYVIKRHDGFVLKFVGDAVIGYFVAEELSTSVASRAVSCAESMLKIIKVGINPILKSNNLPELKIKIGIDYGENTIVRYGDDLQEAHVDILGPSVSMAAKIQNLAQSDQIMVGGDVYAKLHGSIQEYFVNLDLEKSNWDYKSAKTKKVYPVYAYVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR50</accession>
    <name>A0A2S2KR50_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876723.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR50"/>
    <dbReference type="GeneID" id="76208785"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="224" mass="25740" checksum="81DD0F43CC0A68D7" modified="2018-07-18" version="1">MKLEHAIIISVGVLVGTSGLLISIEPNTIPHWEALLAIKQFHDLGCDKECKLKWESDGFACVKTTENEHVCRPPREIFYPDREVQVRTAFPSEYGEFLYFPDGLTTDDGRLFDIHKVDLINHDTKQIRIEFANHNTSPPELQFEYYANLVQGQTFVSHCTGTDRKMGHVVEYLDTFEIDGQTYIEFWGSHVKMPDALLPCQMPELIEHSIPYDLSLGIQFEEYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR72</accession>
    <name>A0A2S2KR72_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Putative [LysW]-aminoadipate/[LysW]-glutamate kinase</fullName>
            <ecNumber evidence="9">2.7.2.17</ecNumber>
            <ecNumber evidence="9">2.7.2.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">lysZ</name>
        <name evidence="11" type="ORF">NZNM25_09400</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in both the arginine and lysine biosynthetic pathways. Phosphorylates the LysW-bound precursors glutamate (for arginine biosynthesis), respectively alpha-aminoadipate (for lysine biosynthesis).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[amino-group carrier protein]-C-terminal-N-(1,4-dicarboxybutan-1-yl)-L-glutamine + ATP = [amino-group carrier protein]-C-terminal-N-(1-carboxy-5-phosphooxy-5-oxopentan-1-yl)-L-glutamine + ADP</text>
            <dbReference type="Rhea" id="RHEA:41944"/>
            <dbReference type="Rhea" id="RHEA-COMP:9694"/>
            <dbReference type="Rhea" id="RHEA-COMP:9712"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:78499"/>
            <dbReference type="ChEBI" id="CHEBI:78503"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[amino-group carrier protein]-C-terminal-gamma-(L-glutamyl)-L-glutamate + ATP = [amino-group carrier protein]-C-terminal-gamma-(5-phospho-L-glutamyl)-L-glutamate + ADP</text>
            <dbReference type="Rhea" id="RHEA:52632"/>
            <dbReference type="Rhea" id="RHEA-COMP:13311"/>
            <dbReference type="Rhea" id="RHEA-COMP:13313"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:136714"/>
            <dbReference type="ChEBI" id="CHEBI:136717"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; L-arginine biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (Thermus route): step 2/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the acetylglutamate kinase family. LysZ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.17" evidence="9"/>
    <dbReference type="EC" id="2.7.2.19" evidence="9"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876774.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR72"/>
    <dbReference type="GeneID" id="76208730"/>
    <dbReference type="OrthoDB" id="6816at2157"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00036"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003991">
        <property type="term" value="F:acetylglutamate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043744">
        <property type="term" value="F:N2-acetyl-L-aminoadipate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04251">
        <property type="entry name" value="AAK_NAGK-UC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02082">
        <property type="entry name" value="LysZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004662">
        <property type="entry name" value="AcgluKinase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037529">
        <property type="entry name" value="LysZ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00761">
        <property type="entry name" value="argB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010659">
        <property type="entry name" value="PRK14058.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010662">
        <property type="entry name" value="PRK14058.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342:SF0">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000728">
        <property type="entry name" value="NAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 9" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 9" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/glutamate/uridylate kinase" evidence="10">
        <location>
            <begin position="4"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="37"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="9">
        <location>
            <position position="5"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="9">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="267" mass="28655" checksum="E5942CF80E06E8A0" modified="2018-07-18" version="1">MITIKIGGSVVDNLHPSTISDIKKVAESEGLILVHGGGKEVTKVCEELGKEPKFVTSPSGIKSRYTDKETAEIFTMVMSGRINKTIVQMLQKNGINAIGLSGVDAKIIQADRKKKLLIVNEKGRKQAIDGGYTGKITDINSNFIKLLLEQGLTPVISPIAISEESEFLNVDGDRAAAYIAGKVGCDKVLFITNVDGLLMDDKLVPKLTLAEAKEIRPKIGPGMEKKILASTEALDMGVKKALIANGQKENPISSAIAEDSCTVIEHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR74</accession>
    <name>A0A2S2KR74_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Kazal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_09050</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876742.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR74"/>
    <dbReference type="GeneID" id="76208765"/>
    <dbReference type="OrthoDB" id="12245at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000923">
        <property type="entry name" value="BlueCu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052721">
        <property type="entry name" value="ET_Amicyanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507:SF1">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00127">
        <property type="entry name" value="Copper-bind"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00050">
        <property type="entry name" value="Kazal_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="5">
        <location>
            <begin position="33"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="81"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="347" mass="37301" checksum="B6AE0727262D941F" modified="2018-07-18" version="1">MRLDFSVGVIVIVGVLAAVSIGFIAMNPDEIIQPRMVSVDEPTVCTLEWNPMCGVDGETYGNLCMLNAADVNLDYAGECMVAEPTPEPEPTPEPEPTPEPEPTPEPTMTMPVATTGVHTVNIAEGSSIPGCEETNECYLPSLITISVGDIVTWNNPDSAAHTVTSGNISDGHDGVFDSGLFMSGSTFEFTFDESGTFDYFCMVHPWMIGQVIVNDVEEMVVIEEPTSEPIMESEPTMEMEPEVIAEPEKTSIVSIPPGAAIPGCDETNECYLPYEITISVGQSVTWSNDDSAAHTVTSGTVNAGVTGVFDSGLFMSGSTFEHTFEKAGKYDYFCMVHPWMTGKVIVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRA3</accession>
    <name>A0A2S2KRA3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRA3"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="49" mass="5880" checksum="8DFA985A7C153364" modified="2018-07-18" version="1">MLMKKSKFVGITIPKELYDKIEKKRGDLPRSYIYKKLVETGYEQGELKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRC1</accession>
    <name>A0A2S2KRC1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_07960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876650.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRC1"/>
    <dbReference type="GeneID" id="76208880"/>
    <dbReference type="OrthoDB" id="10422at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012666">
        <property type="entry name" value="CbtA_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09490">
        <property type="entry name" value="CbtA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="244" mass="26719" checksum="1CC84FCD62BC25EF" modified="2018-07-18" version="1">MRTVLFLAIVLVSGAIAGTFHGMANLAIVEPYLDQAIGIENQNLFASGEEEDTPQFWVEYNSYRDWQKGGQVLAGAILGTSIGALFGIVYALSRNSLPGKTDLQKSFVLAGIMWISIYLIPFLKYPANPPTVGDAETVVLRAILYLSFIAISGFGVIGFYQVYKRLKKNTQVFAFVGYIAFIGIVFALMPANPDEVTAPMDLVNGFRAMSVVAVSVFWVSVAAIMGMLWHKFRPDSEISVEKTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRC8</accession>
    <name>A0A2S2KRC8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRC8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="309" mass="34126" checksum="59C6279479D73159" modified="2018-07-18" version="1">MIQPSYGDVESPRKQMERGVSAADVMCKAGLERVIRTNGDAACVKPDTAERMKKAGMLLIPINFSDLQKDIQSVTASEKEMQTVPASSMSIVNFYITDQDLNTAHSGIEVVPIQGLFEFTINGIPIEGPKNMIETGPNTGEFYIRLELPDTINGRPLSQDDIVLIKYLDESDYSGEQRILVKSVPLTKTFAKVESSGGGSRIGHEFTVRIYEPDANRDSKDEDKIPLSSLEYRGDGGIRTTLANPRFDANSSYLIETGPNTSTFEVKIKIPRELDGKVIHIGDSYEIRYIDRSTPSGTDEKIILKGRIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRD6</accession>
    <name>A0A2S2KRD6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866843.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRD6"/>
    <dbReference type="GeneID" id="76208868"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="157" mass="17760" checksum="474B63048ACCF21B" modified="2018-07-18" version="1">MNVVSKYSIQRNGKTRKSLSTACIFVMMLSMSVPLFDDAVFAESTKTTVTELDDETSLEMTTLSMNIPETNTLPWGTIKGKVNDPSQGYPVIIQIFKPDEDVPIHVAQINLKGDHSFEYKFRLLSIDEGNITRFFEGDYNVKIFKVINTPRDNLDSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRF0</accession>
    <name>A0A2S2KRF0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_10430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRF0"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="144" mass="16579" checksum="9D0AC54D84FE8FAF" modified="2018-07-18" version="1">MKTRTKIPIIIIASMVLLFLLYFFYNAPIICDDVIFQLNLCGDHLEEKYVPLPIIQHFMQLYPETTGLGYSTGMYQVQKVFADAMLAEKIFVRLEIDLRDSSIIYSCSNHNKGDDFLVSYITNPTVNDLDSNNCRTLNNLILEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRF3</accession>
    <name>A0A2S2KRF3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_10370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876856.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRF3"/>
    <dbReference type="GeneID" id="76208628"/>
    <dbReference type="OrthoDB" id="9357at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="397" mass="45937" checksum="FCC9382E21D23A70" modified="2018-07-18" version="1">MGEFEEFAEALFGQLSVEINEEKEIMQLAARAREDIAGKVQFRELEEIAKEIFPSFKGKVEDFLGIKLADNLQLKFPELEDLKRLKGDKVFADSESKEFVKKLFNAVAREDLKTIAELMKENTAKYLVYSTYAIQYISKITTTYGDYLDSIIYLNKFILSRYPQIILHKQGEPYESRFENVNSGYLGAVKMTVLEELIHSAQGNLHQINKNAAMEVNKINEELAKIILSLDTETINKLSEYCQLQAVPDDFPFAKKANLFFFLNPDHFLIEQIGPDVMTFTHVEIDPKIDESIPHLLDIYKRWLVPIQQHHAAFTTMEGMAGFAIENILKEDQDFQNYLTTFMGTDFSSYQVRKSMGKDFTKTIYEKLGRNTFKKMMELPPNTRELKDPQLYLKKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRL0</accession>
    <name>A0A2S2KRL0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA-processing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_10870</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876939.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRL0"/>
    <dbReference type="GeneID" id="76208572"/>
    <dbReference type="OrthoDB" id="7870at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22389">
        <property type="entry name" value="KH-I_Dim2p_like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22390">
        <property type="entry name" value="KH-I_Dim2p_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000076">
        <property type="entry name" value="KH domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000119">
        <property type="entry name" value="KH type 1 domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019964">
        <property type="entry name" value="KH_domain_protein_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055211">
        <property type="entry name" value="KH_PNO1_2nd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03665">
        <property type="entry name" value="arCOG04150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010331">
        <property type="entry name" value="PRK13763.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826">
        <property type="entry name" value="RIBONUCLEASE Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826:SF13">
        <property type="entry name" value="RNA-BINDING PROTEIN PNO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22891">
        <property type="entry name" value="KH_PNO1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="84"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="190" mass="20846" checksum="41600D5643A21AAB" modified="2018-07-18" version="1">MSFEKLIRIPNDRIAVLIGKSGNVKSKIESICHVSLDIDGETGEVFIKSQGDVEKIQPFKAMEIVTAIGRGFSPENAMTLLQGENALHVIDLREFAGKSNANVERIKGRIIGEGGRARRNMENLSGTHISVYGKTVSIIGDSSKLRLAVDAISSISSGSMHGAVYTKLEAANRRDKQEKMKLWEDQDVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRL4</accession>
    <name>A0A2S2KRL4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829073.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRL4"/>
    <dbReference type="GeneID" id="76208547"/>
    <dbReference type="OrthoDB" id="8772at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="55" mass="6320" checksum="9D23F33502C34D80" modified="2018-07-18" version="1">MVSKEKCAICSGKIQLRFKPMSEWGIEGLICGNCYSKKINEHYPGEHIRVNKHLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRM4</accession>
    <name>A0A2S2KRM4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ArnR1-like winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRM4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038723">
        <property type="entry name" value="ArnR1-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14947">
        <property type="entry name" value="HTH_45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ArnR1-like winged helix-turn-helix" evidence="1">
        <location>
            <begin position="8"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="96" mass="10631" checksum="032B6694F283A20B" modified="2018-07-18" version="1">MYDMVSYRTSMQIVADLLTATEQSGQEGIKTTSLLTKANLSHSRLSKFLQNLTGAGLINKIEFDGKNTFVITPKGRQYLESYANFSSIAESFGLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRM6</accession>
    <name>A0A2S2KRM6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Double-stranded DNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PDCD5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876966.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRM6"/>
    <dbReference type="GeneID" id="76208536"/>
    <dbReference type="OrthoDB" id="9122at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.140">
        <property type="entry name" value="PDCD5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002836">
        <property type="entry name" value="PDCD5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036883">
        <property type="entry name" value="PDCD5-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01984">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="15632" checksum="0D36611A1BD5B9F9" modified="2018-07-18" version="1">MSEEDFELERLKAKRLAEMQKNISSRQATNESPDTKKKIITKPRDSLVKILGFRGLEVLENAETQFPNETKLIVEKLSELIKTGEINETLDGGKLLMLFRSVGLNVRMETKINVEQDGKFVSLSDKLSSKSSQNSDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRP3</accession>
    <name>A0A2S2KRP3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thiamine-monophosphate kinase</fullName>
            <shortName evidence="1">TMP kinase</shortName>
            <shortName evidence="1">Thiamine-phosphate kinase</shortName>
            <ecNumber evidence="1">2.7.4.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thiL</name>
        <name evidence="3" type="ORF">NZNM25_11170</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent phosphorylation of thiamine-monophosphate (TMP) to form thiamine-pyrophosphate (TPP), the active form of vitamin B1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>thiamine phosphate + ATP = thiamine diphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:15913"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine phosphate: step 1/1.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Reaction mechanism of ThiL seems to utilize a direct, inline transfer of the gamma-phosphate of ATP to TMP rather than a phosphorylated enzyme intermediate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiamine-monophosphate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.16" evidence="1"/>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876963.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRP3"/>
    <dbReference type="GeneID" id="76208540"/>
    <dbReference type="OrthoDB" id="45909at2157"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00142"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009030">
        <property type="term" value="F:thiamine-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02194">
        <property type="entry name" value="ThiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02128">
        <property type="entry name" value="TMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006283">
        <property type="entry name" value="ThiL-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01379">
        <property type="entry name" value="thiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270">
        <property type="entry name" value="THIAMINE-MONOPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270:SF0">
        <property type="entry name" value="THIAMINE-MONOPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005303">
        <property type="entry name" value="Thiam_monoph_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="2">
        <location>
            <begin position="30"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="117"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="315" mass="34777" checksum="1FCA911AA02F2018" modified="2018-07-18" version="1">MIKLDESSIIKIFQKGLGNNEFVSEDVEIFNLGKIKIISKTDTLVESTDIPPKMKLTDAARKSIIACVSDFAAKGIKPEYGIISVNLPKKISRSKILDIVKGFKKASSEYGIKILGGDTNAGKEIVFNVTLFGNSKNIVTRKGSKKGDLIFVTGPFGYTSVGLDLLLGKRKESGKFAVKSIKSMINPKPKLKFGLKNKKYFSSSMDSSDGLSTTLNEMSKQSKKKFLINNIPAGNDLENYAKSHKLDLNSLVFHGGEEYEFVFTVSPKYKQIIIKNARSLKTPIIQIGQVTSGKGVFVKAEKDYVTLKDLGWKHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRT1</accession>
    <name>A0A2S2KRT1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tryptophan synthase beta chain-like PALP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877030.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRT1"/>
    <dbReference type="GeneID" id="76208479"/>
    <dbReference type="OrthoDB" id="10138at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="1">
        <location>
            <begin position="58"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="515" mass="56682" checksum="E78F50C3D795EEDB" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRU3</accession>
    <name>A0A2S2KRU3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_09590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRU3"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="62" mass="7206" checksum="C1D59AFF3B01F79D" modified="2018-07-18" version="1">MYVHFLHTKEYHADLPYFVVVNTYCHLCRGGGKDCIDVNENQDYAMRDIDPKCHICGKSLID</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRU4</accession>
    <name>A0A2S2KRU4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps15</name>
        <name evidence="8" type="ORF">NZNM25_11640</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877000.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRU4"/>
    <dbReference type="GeneID" id="76208488"/>
    <dbReference type="OrthoDB" id="6533at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.10:FF:000003">
        <property type="entry name" value="40S ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_A">
        <property type="entry name" value="Ribosomal_uS15_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_uS15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023029">
        <property type="entry name" value="Ribosomal_uS15_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012606">
        <property type="entry name" value="Ribosomal_uS15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006331">
        <property type="entry name" value="PRK08561.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885">
        <property type="entry name" value="RIBOSOMAL PROTEIN S15P/S13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885:SF6">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08069">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01386">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00362">
        <property type="entry name" value="RIBOSOMAL_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS15 N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="149" mass="17291" checksum="1E2900531C3228E2" modified="2018-07-18" version="1">MGRMHTHRHGKSHSIRPATLRAPSWITQSPKEIEELVVKYSKDGLTPSQIGIKLRDQHSIPLIKPITKKSMGDILKENDLRPEMPEDLENMVKKAVGLQKHLKENKGDNRNVRSLELIEAKVHRLSVYYKRIERIPKTWKYKSVVAQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRU5</accession>
    <name>A0A2S2KRU5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Serine--tRNA ligase</fullName>
            <ecNumber evidence="7">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Seryl-tRNA synthetase</fullName>
            <shortName evidence="7">SerRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Seryl-tRNA(Ser/Sec) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">serS</name>
        <name evidence="11" type="ORF">NZNM25_11670</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>tRNA(Sec) + L-serine + ATP = L-seryl-tRNA(Sec) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42580"/>
            <dbReference type="Rhea" id="RHEA-COMP:9742"/>
            <dbReference type="Rhea" id="RHEA-COMP:10128"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>tRNA(Ser) + L-serine + ATP = L-seryl-tRNA(Ser) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9669"/>
            <dbReference type="Rhea" id="RHEA-COMP:9703"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer. The tRNA molecule binds across the dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="7"/>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877003.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRU5"/>
    <dbReference type="GeneID" id="76208485"/>
    <dbReference type="OrthoDB" id="35932at2157"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000048">
        <property type="entry name" value="Serine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="entry name" value="Serine-tRNA synthetase, tRNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00176">
        <property type="entry name" value="Ser_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="10">
        <location>
            <begin position="138"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="229"/>
            <end position="231"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <begin position="260"/>
            <end position="262"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="276"/>
            <end position="279"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <begin position="347"/>
            <end position="350"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="381"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="383"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for serine binding" evidence="8">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="421" mass="48651" checksum="2424519DF4B87C9B" modified="2018-07-18" version="1">MLDPKLIKENPQIIRDMLKARSVEFDLNGLIESDQKRREFILKTDELRKKKNQVASEISLKKKKGEDTSGILKEMKNVSAKLSELESEQESIKKYYSKLALTIPNLVDKSVPIGIDESSNIEIRKWGKIPNFNFKVNDHIDISENLDLVDLERAAKVAGARFYYLKNDLVRLNQSLIHYGLNFLAKKEYSLVQPPYMINRESMEGAVIADDFEDVIYKVEDEDLYMIGTSEHAMAAMHSKEIIEGKDLPLRYAGISPCFRKEAGAHGRDQKGIFRVHQFDKIEQFVFSRPEDSWNEHEKMISVAEEFYQSLEIPYRVMLLSSGDMGKVSAKTYDIEAWMSGQNAYREIVSCSNCLDYQARRLKIRFRDKTNEDTQYLHTLNSTLIATTRILVAIMENFQTKDGHIKIPYVLQSYMGNQKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRW9</accession>
    <name>A0A2S2KRW9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Histone</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_11080</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal histone HMF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876957.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRW9"/>
    <dbReference type="GeneID" id="76208550"/>
    <dbReference type="OrthoDB" id="7514at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22909">
        <property type="entry name" value="HFD_archaea_histone-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050947">
        <property type="entry name" value="Archaeal_histone_HMF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050004">
        <property type="entry name" value="HmfB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF043032">
        <property type="entry name" value="archaea_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47828">
        <property type="entry name" value="ARCHAEAL HISTONE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47828:SF1">
        <property type="entry name" value="ARCHAEAL HISTONE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="7">
        <location>
            <begin position="8"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="73" mass="7969" checksum="2CA9841561D197F6" modified="2018-07-18" version="1">MRSSELGLSAMYRILKKAGAERVSDESADELRRAIEEIADSIAKSAVDMASHAGRKTVKGEDVKLASKPFNKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRY6</accession>
    <name>A0A2S2KRY6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">N5-carboxyaminoimidazole ribonucleotide synthase</fullName>
            <shortName evidence="4 6">N5-CAIR synthase</shortName>
            <ecNumber evidence="4 6">6.3.4.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4 6">5-(carboxyamino)imidazole ribonucleotide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 6 8" type="primary">purK</name>
        <name evidence="8" type="ORF">NZNM25_12000</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).</text>
    </comment>
    <comment type="function">
        <text evidence="6">Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 6">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + hydrogencarbonate + ATP = 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:19317"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 6">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 6">Belongs to the PurK/PurT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.18" evidence="4 6"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877049.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRY6"/>
    <dbReference type="GeneID" id="76209306"/>
    <dbReference type="OrthoDB" id="9299at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00942"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034028">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004638">
        <property type="term" value="F:phosphoribosylaminoimidazole carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01928">
        <property type="entry name" value="PurK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003135">
        <property type="entry name" value="ATP-grasp_carboxylate-amine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005875">
        <property type="entry name" value="PurK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040686">
        <property type="entry name" value="PurK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054350">
        <property type="entry name" value="PurT/PurK_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004675">
        <property type="entry name" value="PRK06019.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004679">
        <property type="entry name" value="PRK06019.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01161">
        <property type="entry name" value="purK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11609:SF5">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11609">
        <property type="entry name" value="PURINE BIOSYNTHESIS PROTEIN 6/7, PUR6/7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02222">
        <property type="entry name" value="ATP-grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17769">
        <property type="entry name" value="PurK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22660">
        <property type="entry name" value="RS_preATP-grasp-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="1 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 4 6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="7">
        <location>
            <begin position="108"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="179"/>
            <end position="182"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="264"/>
            <end position="265"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="381" mass="42940" checksum="B7DA00086B5E6882" modified="2018-07-18" version="1">MTKVLGIIGGGQLGMMITEAAKKMPQHISKIIVLDPTKNCPASQVGAEQIIADFKDKNAIVDLANKSDIITYEIESGDSTILKNVEKNTKINPSPETLRIIQDKFLQKSFLKENNIPVPEFIKIENIEELKTGLRNFGYPALLKARRDAYDGRGNYKIDSEKDIQKALDYFKGQNLLLEKFVKFKMEVSVIASRNTKGQIKTYPLVENIHEQNILRETIAPARVSHEITKKAEVIAEKTMQVLKGAGIFGIEMFVTQEDDIVINEIAPRVHNSGHHTLQSTKTSQFEQHLRAILGLELGDTELLHPTIMYNILGDVSFEGKYAPINISEENVFLKMYGKEISKPLRKLGHFNLVAKEGETMEELLKKIEKIKEKVVVKQVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRY7</accession>
    <name>A0A2S2KRY7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator TrmB N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_12230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877070.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRY7"/>
    <dbReference type="GeneID" id="76209281"/>
    <dbReference type="OrthoDB" id="11462at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051797">
        <property type="entry name" value="TrmB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="1">
        <location>
            <begin position="34"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="282" mass="32705" checksum="8E38B2A9AD65633E" modified="2018-07-18" version="1">MAKDTQESVFDSIPDSKMYEYKLSVEKVQTELLQYGLTTNQSKVFIYLGKYGSKTAPEVCKSLKLPRTETYHLLSALQNKGIVSATFQHPIQFTALPLNKAIWILVNAEKERVKSLERMEQGLSKLWDNIPVFNSMDSDVEEKFQMLQGSNQIHSKITEMTDSHNDEFLILGSEKDYLKLYHGDFLESFVKSKQKFRLLSGCSDKTTYIFDDLERKNIKKLHNDIKNHLCFIVKDDSEMLFFTKNAMSPEEPFAMWTNSNSMVYAMKLLFESLWSNSKNIHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRZ4</accession>
    <name>A0A2S2KRZ4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Lrp/AsnC family transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877316.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRZ4"/>
    <dbReference type="OrthoDB" id="10897at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="79" mass="8835" checksum="6BA68819E196F78F" modified="2018-07-18" version="1">MRALCLITVKPGKADTVVEILKKKRKIVKQIMVVTGRADISVILQGNIDEINGMVIDFKKIKDIVTTETLIEVEVNLGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS17</accession>
    <name>A0A2S2KS17_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12340</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877081.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS17"/>
    <dbReference type="GeneID" id="76209270"/>
    <dbReference type="OrthoDB" id="9913at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="69" mass="7819" checksum="718FD7B325638855" modified="2018-07-18" version="1">MVLTDKEKMIAIISNGIAVFSLLQERDSLPKNTTMYDFILKIVPENIKSELSVELIDEVFQFVTSTHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS60</accession>
    <name>A0A2S2KS60_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Type 2 DNA topoisomerase 6 subunit B</fullName>
            <ecNumber evidence="7">5.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Type II DNA topoisomerase VI subunit B</fullName>
            <shortName evidence="7">TopoVI-B</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">top6B</name>
        <name evidence="9" type="ORF">NZNM25_10860</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Relaxes both positive and negative superturns and exhibits a strong decatenase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Homodimer. Heterotetramer of two Top6A and two Top6B chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TOP6B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="7"/>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876938.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS60"/>
    <dbReference type="GeneID" id="76208573"/>
    <dbReference type="OrthoDB" id="65493at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00823">
        <property type="entry name" value="TopoIIB_Trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000062">
        <property type="entry name" value="Type 2 DNA topoisomerase 6 subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00322">
        <property type="entry name" value="Top6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005734">
        <property type="entry name" value="TopoVI_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015320">
        <property type="entry name" value="TopoVI_B_transducer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003218">
        <property type="entry name" value="PRK04184.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48444">
        <property type="entry name" value="DNA TOPOISOMERASE 6 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48444:SF1">
        <property type="entry name" value="DNA TOPOISOMERASE 6 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09239">
        <property type="entry name" value="Topo-VIb_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="8">
        <location>
            <begin position="28"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="101"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="532"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00063696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="622" mass="70099" checksum="DB9D4F666A74EDA6" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS69</accession>
    <name>A0A2S2KS69_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">anthranilate synthase</fullName>
            <ecNumber evidence="3">4.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_12870</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the anthranilate synthase component I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877115.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS69"/>
    <dbReference type="GeneID" id="76209213"/>
    <dbReference type="OrthoDB" id="25514at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="entry name" value="Anthranilate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006805">
        <property type="entry name" value="Anth_synth_I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="AMINOBENZOATE/ANTHRANILATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236:SF9">
        <property type="entry name" value="ANTHRANILATE SYNTHASE COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04715">
        <property type="entry name" value="Anth_synt_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00095">
        <property type="entry name" value="ANTSNTHASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="ADC synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Anthranilate synthase component I N-terminal" evidence="7">
        <location>
            <begin position="21"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Chorismate-utilising enzyme C-terminal" evidence="6">
        <location>
            <begin position="183"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="449" mass="50798" checksum="5A6B5D977E015AF8" modified="2018-07-18" version="1">MDIFGNAQAKIIPLDLSENQFQIYNKISRNYSHSFLFESLTGPEVLAETSVMGFDPKIVLKGYSDKVEIISDGKTQTIQTTDPFLELKKILGKSDDQSYRYLGGAVGVVNYDAIRLVENIADSHDSKQPLMEFGIYDDGILYDNVHKKLFYFYHDENRFDKLIMGEDVFEEFHSSEVTPNMDKEKFSEIVNKAKKYIHDGDIFQVVLSRKFAFKNNGDHLTLYKTLRKLNPSPYMYHLKQGRKTIIGASPEMLVRITKDKVETFPIAGTRKITNDEKENKKLADELIHDEKELAEHTMLVDLGRNDIGRVCKYGTVHPESLMQIKRFSHVQHIVSHVIGNLDPKNDMFDAFQAVFPAGTVSGAPKVRAMEIIDELEPEARGPYAGAVGYFSYNGCCDFAIAIRSIFIEDNEGFIQSGAGIVSDSIAENEFRETEHKAGAMLQALREASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS94</accession>
    <name>A0A2S2KS94_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877075.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS94"/>
    <dbReference type="GeneID" id="76209276"/>
    <dbReference type="OrthoDB" id="11245at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="87" mass="9808" checksum="685F54035B154BB0" modified="2018-07-18" version="1">MTELSLTDIEFIKILANSDASILQIGMNDATRKRLDEQIGVILREYYRENTMNLKTGWTEKFLKAGINEDDGKSALACARRLGLDIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSA0</accession>
    <name>A0A2S2KSA0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Type II secretion system protein GspF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_13330</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877154.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSA0"/>
    <dbReference type="GeneID" id="76209165"/>
    <dbReference type="OrthoDB" id="12374at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056569">
        <property type="entry name" value="ArlJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402:SF1">
        <property type="entry name" value="TYPE II SECRETION SYSTEM PROTEIN GSPF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Type II secretion system protein GspF" evidence="7">
        <location>
            <begin position="132"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="306" mass="33790" checksum="195375F2FCF8165F" modified="2018-07-18" version="1">MNVTEKKQKKSEEESVGQIHVYSYKLLNEHVKFLHPKLKSLDKSIKQAMMPIPFEVYVSSMIFFSMIAGACGAVLGFVTAQFINIQPASLEILLPVLGGLVLFGITFGILQIIPKVRVTNRSSKLIEEIPHFIGYMSTLATSGLTLEGIFKAIAKEETEEDIVKDARYIVRNIDILGMDLISAIKDLIHRTPTGPYSELLEGAVVTVQSGGDLKEYFNATAKVQLEEKKMLMQKTTESLGSVAEIYTILLIVFPLLAVIMLSIMGIMSPSLAGFDLITLMNILTFAVIPLSGVLMLVMMDTMVPKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSD6</accession>
    <name>A0A2S2KSD6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13470</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSD6"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="93" mass="11157" checksum="029017A208EB2CCF" modified="2018-07-18" version="1">MFMVTISEKDIEEIITYLEKSIMNLTKQTLENFETGGEFQDTRKFLENQFEIRLENLLIAKNSSTHHLESGMKNRIIQRKQKIFEKISKQYRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSD7</accession>
    <name>A0A2S2KSD7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ammonia monooxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876998.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSD7"/>
    <dbReference type="GeneID" id="76208493"/>
    <dbReference type="OrthoDB" id="6824at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.570">
        <property type="entry name" value="Particulate methane monooxygenase, b subunit. Chain: A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006833">
        <property type="entry name" value="NH3_CH4_mOase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023301">
        <property type="entry name" value="NH3_CH4_mOase_suB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010916">
        <property type="entry name" value="TonB_box_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04744">
        <property type="entry name" value="Monooxygenase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00430">
        <property type="entry name" value="TONB_DEPENDENT_REC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="189" mass="20620" checksum="4C9D25C50C2C488A" modified="2018-07-18" version="1">MVEKRIFVFGLAVVLALGTLGFNWVESVIPTADAHGVQAQLQSRFIRIEDETFNRQSLQTGETLTVQGTLVSLVERDLRGWLSIFTESTNAGNRWEMLARDPPGNVFDIPGNAVVDYSLSAKALEAGVYHVHTQLNVAKVGPGLGPGQTVVVEGDPIIKPIPYTNIAYQSIIIGVGYVITFATRPWQVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSF8</accession>
    <name>A0A2S2KSF8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nitrosopumilus output domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_13450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSF8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041213">
        <property type="entry name" value="NitrOD1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18549">
        <property type="entry name" value="NitrOD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitrosopumilus output" evidence="1">
        <location>
            <begin position="2"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="65" mass="7365" checksum="E106E534FF6584D0" modified="2018-07-18" version="1">MFKGDKKSEIALLTFEQYSNIKDLPITKECRIVKNEKPTLSKNDVAAINKKIAKLSKTHTQSLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSG2</accession>
    <name>A0A2S2KSG2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Methylmalonyl-CoA mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_13700</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877184.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSG2"/>
    <dbReference type="GeneID" id="76209129"/>
    <dbReference type="OrthoDB" id="9041at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02071">
        <property type="entry name" value="MM_CoA_mut_B12_BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.280">
        <property type="entry name" value="Cobalamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006159">
        <property type="entry name" value="Acid_CoA_mut_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00640">
        <property type="entry name" value="acid_CoA_mut_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101:SF1">
        <property type="entry name" value="METHYLMALONYL-COA MUTASE, LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101">
        <property type="entry name" value="METHYLMALONYL-COA MUTASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="Cobalamin (vitamin B12)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="5" id="KW-0170">Cobalt</keyword>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B12-binding" evidence="6">
        <location>
            <begin position="9"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="140" mass="14940" checksum="090C45C4BBB06714" modified="2018-07-18" version="1">MKQKVATRSIKILVAKLGLDGHDRGALVLCRAFRDAGMEVIYSGLFATPERIAQIAEDEDVDAVAMSLLNGAHGTLFPRVVKALKKKGINDVLIVGGGVIPDIDHKDLIKAGVDHVFGPGTPLPTIINHITTGVSKLRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSH7</accession>
    <name>A0A2S2KSH7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13970</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866897.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSH7"/>
    <dbReference type="GeneID" id="76209102"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.10">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="98" mass="11583" checksum="C429445E8E45E172" modified="2018-07-18" version="1">MIEMLENEWSDWLDCNQQVLLEIPEKPGVYMMHAAMKILLIGGSHNMKKSISEALEQKCVSKATRVRFREEENFEEIRKELITDYKKRHEGNIPQCMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSI4</accession>
    <name>A0A2S2KSI4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13940</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSI4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="119" mass="13567" checksum="11CDE37C181969CC" modified="2018-07-18" version="1">MPNFILAKVGAGEIIYDLRKKIQDIRSDLNQLGVSPSEIPELIMSANLLRSNEFLSKTNEKQSELISAYEQYSESLEEMLSSVFEIQKDLKDILKEQSLMISQKKTPAKKTSKTKKTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSJ8</accession>
    <name>A0A2S2KSJ8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_14100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866899.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSJ8"/>
    <dbReference type="GeneID" id="76209089"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="77"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="277" mass="31244" checksum="B511F93ADE550495" modified="2018-07-18" version="1">MISINPFHALLWTFRRNEKDVVNLYNTLSPVMQLATGGSMLNFGYWSSCNVDPISAQNNLCLIFGDLAELSSAKHVVDVGSGLSAPSKLWGDVFPNLELYDVNINFNQLISSGTQKNIDLLNATSTKLPFANNSIDRVLALESAQHFRPLSDFICESKRILRTSGLLVIAIPITTGNSSFGKLGVLKFTWSSEHYSLDYLKNLLRDAGFSISDEKYIGSDVYPPLTNYYLENRERLKKLILENYPKYVEQILFKSLLKMKQASEDKIIDYVLLKCHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSL1</accession>
    <name>A0A2S2KSL1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Arginase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_14370</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="4">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the arginase family. Agmatinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSL1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033389">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via agmatine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="ARGINASE/AGMATINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358:SF26">
        <property type="entry name" value="GUANIDINO ACID HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036979">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR036979-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="272" mass="30164" checksum="3263D4B972D41C13" modified="2018-07-18" version="1">MIIGISDESHSHSLRKGTSEAPDRIRKISRTRDVYVENNKESLAYTNSGKAQSKIFDLGNISRENIPLVYERITYDGKVPITIGGDHSLTASIIQGLGNKRGPISLVYFDAHPDFLSSTRNFYGSVVYDCLPYIDPKSSVMIGIRSPELEEIENIQKYGITVFSPADIQYYGFKQVIEDILSKIGKNIYVSFDMDCIDPAFAPGVSVPVPMGLNPITASILLKSITQDGILGMDMMEVCPKFDIQDNTSHLASRMICELLSSVKVKPKLPNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSM7</accession>
    <name>A0A2S2KSM7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_14630</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829092.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSM7"/>
    <dbReference type="GeneID" id="76209032"/>
    <dbReference type="OrthoDB" id="5783at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="45" mass="5587" checksum="4C1C6B9CECE20A1F" modified="2018-07-18" version="1">MEQGQKNNEFYEDCTKYFEFLRKKGTVDYEFEDEYYFTMPAISNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSN2</accession>
    <name>A0A2S2KSN2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cupin type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_14250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877232.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSN2"/>
    <dbReference type="GeneID" id="76209070"/>
    <dbReference type="OrthoDB" id="82049at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43698:SF1">
        <property type="entry name" value="BLL4564 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43698">
        <property type="entry name" value="RIBD C-TERMINAL DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-1" evidence="1">
        <location>
            <begin position="40"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="151" mass="17325" checksum="F4AA3B5DA6F52226" modified="2018-07-18" version="1">MKKTNIFGTGDQRKVNPDWFTGKTWMKVISEKIKSKDQDIYHVHFEKGSRTKLHQHDGNQVLIAVKGNGSLEIFKKYGTSKTNFKIKRTEKISLNEGDVVHIPANVLHTHGSVDKKKTFSHIAINILPKKNAPYKTTWYESDFKSKVSEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSQ5</accession>
    <name>A0A2S2KSQ5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PD-(D/E)XK endonuclease-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_12690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSQ5"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038726">
        <property type="entry name" value="PDDEXK_AddAB-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31340">
        <property type="entry name" value="MITOCHONDRIAL GENOME MAINTENANCE EXONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31340:SF3">
        <property type="entry name" value="MITOCHONDRIAL GENOME MAINTENANCE EXONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12705">
        <property type="entry name" value="PDDEXK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PD-(D/E)XK endonuclease-like" evidence="1">
        <location>
            <begin position="95"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="215" mass="25310" checksum="AAEDE7252E073A46" modified="2018-07-18" version="1">MLKIREPELLDRADGHWYKTKFDAIYPSISTILSATSSDEKQNILNAWRENEPAHEYITAQAQDIGTQSHKIIENYLNNCLSLEEFDLLPVAHFNNLKPYLEHISDVTSIEQRMYSDKLKVAGTSDLIARYNGELSIIDYKTKRKPQRDEYMYEYYLQTTCYAQMFHEVTGRRINQVVILVSSEKNTRQEFIKSCDDYVEPLNERIEKYYLNSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSR5</accession>
    <name>A0A2S2KSR5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF192 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_14550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877257.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSR5"/>
    <dbReference type="GeneID" id="76209040"/>
    <dbReference type="OrthoDB" id="6763at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1140">
        <property type="entry name" value="Protein of unknown function DUF192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003795">
        <property type="entry name" value="DUF192"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038695">
        <property type="entry name" value="Saro_0823-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37953">
        <property type="entry name" value="UPF0127 PROTEIN MJ1496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37953:SF1">
        <property type="entry name" value="UPF0127 PROTEIN MJ1496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02643">
        <property type="entry name" value="DUF192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="163" mass="18427" checksum="AB432CEB3BEE2838" modified="2018-07-18" version="1">MTTRSQVLIPIFIAAVIIGIVGLMSIPSESKLESVEFPRGTIKVDDVPLQVQIADTEPRRVRGLMFQDELPYDQGMIFVFEQSGLYSLWMLNMQFSLDMIWFDEDGKVVHIEKNIPPCKSPLEITTCQSVIPDNEAIYVLEVTSGFVERNNITKDSMLTIISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSW7</accession>
    <name>A0A2S2KSW7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_13290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877152.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSW7"/>
    <dbReference type="GeneID" id="76209169"/>
    <dbReference type="OrthoDB" id="11862at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="235" mass="25628" checksum="5F8BD0C997CE5B73" modified="2018-07-18" version="1">MKSKNIVDTYEMRKNSRLKHRRGVTDIISTMMLMAVTVTGASTLTYFMNDAFLSGNLGTTSTLEASSLNILLLAYDTRDSSRLLTLNDVDNKYDSVLCRSSCSGNPNDLPLNGGTEFIVFQIQNNNLNSIFLERISINGIYYSWDAATSGVVLDTTVPLTNGKYPADGMFSILPISAGSIIQNEDTQIQSGQTVNILVKLDSVDPDIQLNKGIRLLLNSGQIQPAEFLIESGGAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSY8</accession>
    <name>A0A2S2KSY8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-hydroxy-3-methylglutaryl coenzyme A reductase</fullName>
            <shortName evidence="3">HMG-CoA reductase</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_15540</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the HMG-CoA reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877386.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSY8"/>
    <dbReference type="GeneID" id="76208434"/>
    <dbReference type="OrthoDB" id="10981at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004420">
        <property type="term" value="F:hydroxymethylglutaryl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015936">
        <property type="term" value="P:coenzyme A metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00644">
        <property type="entry name" value="HMG-CoA_reductase_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.770.10">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl-coenzyme A Reductase, Chain A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002202">
        <property type="entry name" value="HMG_CoA_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004553">
        <property type="entry name" value="HMG_CoA_Rdtase_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023074">
        <property type="entry name" value="HMG_CoA_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023076">
        <property type="entry name" value="HMG_CoA_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009023">
        <property type="entry name" value="HMG_CoA_Rdtase_NAD(P)-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009029">
        <property type="entry name" value="HMG_CoA_Rdtase_sub-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00532">
        <property type="entry name" value="HMG_CoA_R_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572:SF24">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00368">
        <property type="entry name" value="HMG-CoA_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00071">
        <property type="entry name" value="HMGCOARDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55035">
        <property type="entry name" value="NAD-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56542">
        <property type="entry name" value="Substrate-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01192">
        <property type="entry name" value="HMG_COA_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50065">
        <property type="entry name" value="HMG_COA_REDUCTASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="418" mass="44628" checksum="9340F0650864C2C2" modified="2018-07-18" version="1">MADSSISKLFEKSRKERLDIVANFANLSNDEIKILENENGGISFDKADKMVENAIGTFSLPLGIATNFKINGKDYIIPMVIEEPSVIAAASKGAKVARIKGGFEVSAIESYSIGQIQVLDVDIPTAIKQIENSSDEILKLANSKSNTLSKMGKGAKEISCKEIDTPSGKMLIVELLIDVGDAMGANVTNTMCEAVSPLIENISGGRTLLRILSNYSTRRIAKARAIFEKDAVGGEEVVDNIILAYEFADNDVYRAVTHNKGIMNGIVAVANAVGQDSRAIEAAANAYAAESGRYCSLSKWSKDDEGNLIGTLEIPLSVGIVGGIANVHPVAKVCTKILGVSSAQELACIITATGLAQNYSAIRALSTEGIQKGHMRLHARNLAAAAGATTEQIDDIVQKMIQEKKISLDRAKELLEQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSZ5</accession>
    <name>A0A2S2KSZ5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Haloacid dehalogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_15500</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877383.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSZ5"/>
    <dbReference type="GeneID" id="76208438"/>
    <dbReference type="OrthoDB" id="8384at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01427">
        <property type="entry name" value="HAD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050155">
        <property type="entry name" value="HAD-like_hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43434">
        <property type="entry name" value="PHOSPHOGLYCOLATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43434:SF1">
        <property type="entry name" value="PHOSPHOGLYCOLATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="324" mass="36205" checksum="8D0D22357EC2BCDC" modified="2018-07-18" version="1">MTLTKKFDGIYFDDSSVDLLDGIDAVIFDCDGVLIDITKSYDSAIIKTTKYILETFAKINDSVDVDFKIIDGFKSTGGFNDEVDLTYAAIISLVAAKKLQKDPYDFIFDVIKNADSTGIASVEKYLDALTDISEIKKQLSYPGPHHENILYQNFDQIFYGPELYEKLFAKSSKFTSPGLIEQDDVILNDELLEKLQNKFGSKIAMVTGRGKESVSYSLKELINKFDLKNSAFLEDEPRELAKPNPKSLLDSISGMNSVSTLYVGDSMEDFIMAKKVTDLGYKTIFCGIMGTSKNPKEKLELFERNGAILVLDSINLLPKVLNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT18</accession>
    <name>A0A2S2KT18_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Carbamoyl phosphate synthase arginine-specific large chain</fullName>
            <ecNumber evidence="16">6.3.4.16</ecNumber>
            <ecNumber evidence="5">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Carbamoyl phosphate synthase large chain, N-terminal section</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Carbamoyl phosphate synthase pyrimidine-specific large chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">Carbamoyl phosphate synthetase ammonia chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="ORF">NZNM25_14880</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="29 30">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Large subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The large subunit (synthetase) binds the substrates ammonia (free or transferred from glutamine from the small subunit), hydrogencarbonate and ATP and carries out an ATP-coupled ligase reaction, activating hydrogencarbonate by forming carboxy phosphate which reacts with ammonia to form carbamoyl phosphate.</text>
    </comment>
    <comment type="function">
        <text evidence="20">Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of the biosynthetic pathway leading to pyrimidine nucleotides. The large subunit (synthetase) binds the substrates ammonia (free or transferred from glutamine from the small subunit), hydrogencarbonate and ATP and carries out an ATP-coupled ligase reaction, activating hydrogencarbonate by forming carboxy phosphate which reacts with ammonia to form carbamoyl phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18029"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CarB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="29">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.16" evidence="16"/>
    <dbReference type="EC" id="6.3.5.5" evidence="5"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT18"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004087">
        <property type="term" value="F:carbamoyl-phosphate synthase (ammonia) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1030.10:FF:000002">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000026">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000002">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="entry name" value="Carbamoyl-phosphate synthetase, large subunit oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006275">
        <property type="entry name" value="CPSase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CPSase_lsu_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058047">
        <property type="entry name" value="CPSase_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01369">
        <property type="entry name" value="CPSaseII_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003671">
        <property type="entry name" value="PRK05294.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009455">
        <property type="entry name" value="PRK12815.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF53">
        <property type="entry name" value="CARBAMOYL-PHOSPHATE SYNTHASE [AMMONIA], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405">
        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25596">
        <property type="entry name" value="CPSase_L_D1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="Carbamoyl phosphate synthetase, large subunit connection domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="12 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ATP-grasp" evidence="27">
        <location>
            <begin position="70"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="27">
        <location>
            <begin position="613"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="28">
        <location>
            <begin position="871"/>
            <end position="1017"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00057223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00060037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00062056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00069524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00071108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00074189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00083945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EMBL" id="GBH34697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="1017" mass="113877" checksum="469AFA1B7E9ACB7F" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT40</accession>
    <name>A0A2S2KT40_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_16310</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866921.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT40"/>
    <dbReference type="GeneID" id="76208353"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="247" mass="27421" checksum="A3A2F10E17CA232B" modified="2018-07-18" version="1">MTSKLLLAFTMIFLVFGMTSTAFAHSAKVVGDFKIETGWKNEPPLVGMDNAIEITVSLAEESDKLIYDMIFFNKIDDSTDKATEKHLSGLADEIEVSVSAGGSKTFLLLEENKENLGVYHAKFIPTNTGTHTIHLYGIIKNLEFEMTSQIETVESSDSAQIPDWIRNNAKWWSAGSITDADFVKGIQFLAQQGIIKVEQTTESSVMSQEIPQWIRNNAKWWSAGSITDADFVKGIQFLAQQGIIKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT69</accession>
    <name>A0A2S2KT69_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">4-oxalocrotonate tautomerase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_16330</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 4-oxalocrotonate tautomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877464.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT69"/>
    <dbReference type="GeneID" id="76208351"/>
    <dbReference type="OrthoDB" id="8161at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.429.10">
        <property type="entry name" value="Macrophage Migration Inhibitory Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004370">
        <property type="entry name" value="4-OT-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014347">
        <property type="entry name" value="Tautomerase/MIF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35530:SF2">
        <property type="entry name" value="BSL4019 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35530">
        <property type="entry name" value="TAUTOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01361">
        <property type="entry name" value="Tautomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55331">
        <property type="entry name" value="Tautomerase/MIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4-oxalocrotonate tautomerase-like" evidence="3">
        <location>
            <begin position="2"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="58" mass="6679" checksum="8B2BC112FE0782F3" modified="2018-07-18" version="1">MPLITVSMYPGRTQEQKDEYAKAITKSAVEILKTKENHVIVIFEDNPKENWFLAGNQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTE5</accession>
    <name>A0A2S2KTE5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">tRNA N6-adenosine threonylcarbamoyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.234</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">N6-L-threonylcarbamoyladenine synthase</fullName>
            <shortName evidence="8">t(6)A synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNA threonylcarbamoyladenosine biosynthesis protein Kae1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">kae1</name>
        <name evidence="10" type="ORF">NZNM25_15260</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>L-threonylcarbamoyladenylate + adenosine(37) in tRNA = N(6)-L-threonylcarbamoyladenosine(37) in tRNA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="8">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="8"/>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877364.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTE5"/>
    <dbReference type="GeneID" id="76208462"/>
    <dbReference type="OrthoDB" id="6818at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:tRNA N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000229">
        <property type="entry name" value="tRNA N6-adenosine threonylcarbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01446">
        <property type="entry name" value="Kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034680">
        <property type="entry name" value="Kae1_archaea_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017860">
        <property type="entry name" value="Peptidase_M22_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03722">
        <property type="entry name" value="arch_KAE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF14">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01016">
        <property type="entry name" value="GLYCOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 8" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Gcp-like" evidence="9">
        <location>
            <begin position="36"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="133"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="327" mass="35539" checksum="68177E9B0D4373D5" modified="2018-07-18" version="1">MLGLGIESTAHTFSCAIIEKNGKKGKILSDVRKIYRPAEGEGIHPREASRHHIENSSLVLSECLKEANTSIKELDIVSYAAGPGLGPCLRVGAVVARSLSSFYKIPIYPVNHAIGHIELGKLLTGATNPLVLLVSGGHTMLLAFLNKQWRVFGETLDITLGQLLDQFGRSIGFASPCGKNIEELASTSTNYVTLPYSVKGNDVSFSGLLSATKSVAKKSKTDACYSLQETAFAMISEAVERALSFTQKRELMIVGGVAANKRLSEMLQDVCKRHNCKFFVVPLRYAGDCGSQICWTGLLESQVKSGSSLKDTFVTQSWRLDTVKVNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTH1</accession>
    <name>A0A2S2KTH1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_17330</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTH1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14504">
        <property type="entry name" value="DUSP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000063">
        <property type="entry name" value="Dual specificity phosphatase 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050561">
        <property type="entry name" value="PTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057023">
        <property type="entry name" value="PTP-SAK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23339">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASE AND DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22784">
        <property type="entry name" value="PTP-SAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="8">
        <location>
            <begin position="32"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="9">
        <location>
            <begin position="96"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="175" mass="19869" checksum="6009F27E703ED82A" modified="2018-07-18" version="1">MLKIQQITLHNMSKPGNLWRKVHGRITKKPTNFSWLIEEKLAGSGIPTSFDEFNWLLDQGVKSIVTMTENALPDNWIQDIGYLHVPTPDLTAPDMDKIDLAVDFIHEQIKNNQSVMVHCAAGMGRAGTILACYFVKYKNFTANDAIKKIRDERPGSIQSEVQELAIGFYEKHVRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTH2</accession>
    <name>A0A2S2KTH2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_17610</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877567.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTH2"/>
    <dbReference type="GeneID" id="76208224"/>
    <dbReference type="OrthoDB" id="9818at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="71" mass="8288" checksum="CCD6FCF96A4EFD05" modified="2018-07-18" version="1">MKICNICHKISATDQDHLDCIQKRNIELLDEDFKNNIPERLNLSENTQDLGVEVRAILEHLIKTKDDQDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTL8</accession>
    <name>A0A2S2KTL8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SpoVT-AbrB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_15990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877437.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTL8"/>
    <dbReference type="GeneID" id="76208387"/>
    <dbReference type="OrthoDB" id="40991at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PHOSPHATE-SPECIFIC TRANSPORT SYSTEM ACCESSORY PROTEIN PHOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930:SF2">
        <property type="entry name" value="PHOU DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109755">
        <property type="entry name" value="PhoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="12"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="348" mass="40091" checksum="62335C19ECA78C42" modified="2018-07-18" version="1">MEEREETRKIQFTGKSSYIVSLPKQWIMELGLKQGDQIRMVRKGSSTLELYPPKFESRIQKKEDAVIEIDIEEDASSIIRKLISLYFLGFRTINVKPKSGRLSPNQRNTVKEAVKRMLMGSEIISDSSGGITVQVLVNLLELSVDGAFKRMIHLAKSMSSDAILAVKENNLELAQEVINTDDEVDRFGFYIIRQLKIAIQNEHMLKEMGFRNARNCLGYRLVVKNIERAGDHASFIASDLLEFKKPVKKEILDKLQDMNEFCLSVLDDACLALFKEDYVQAEKTIEKTNEISKYEKKVRDASKSLKDDEEFYRVRRMSENIRRVSEYASDIAEIVLNMNIEKVLKKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTN4</accession>
    <name>A0A2S2KTN4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_16160</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877452.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTN4"/>
    <dbReference type="GeneID" id="76208370"/>
    <dbReference type="OrthoDB" id="2830at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050595">
        <property type="entry name" value="Bact_response_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591:SF3">
        <property type="entry name" value="RESPONSE REGULATORY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591">
        <property type="entry name" value="STRESS RESPONSE REGULATOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="4"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="116" mass="12949" checksum="FCB3935817EC7A62" modified="2018-07-18" version="1">MSKSVIVIDDDEDTVRLFTEFLEERGVNVVGNGFNGTSAVKLFKETNPDVVLIDLNMPNGSGYYAIKKIQEVNPKARIVAVSADSNYTTEEKLEKLNIPLIQKPFKMDQVISIIND</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTN9</accession>
    <name>A0A2S2KTN9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tetrahydromethanopterin S-methyltransferase subunit A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_17180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877533.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTN9"/>
    <dbReference type="GeneID" id="76208267"/>
    <dbReference type="OrthoDB" id="130682at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030688">
        <property type="entry name" value="MeTrfase_MtrA/MtxA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04208">
        <property type="entry name" value="MtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="158" mass="17701" checksum="E46D88E87AC69CBB" modified="2018-07-18" version="1">MNAIGEIIGELCKAILPIPEEYFTGNPNSSIAICTLSSIDLLKKFSNSEILDDIAIVGRLLSENKGIDSIIEYVNSNQKIKTIIVCGKEVWGHKSGDSLFQLHKNGIDKNFKIINSSSPDPYLTVSESKIKYFQNNVKLVNLINETDYETILTKIRFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTU8</accession>
    <name>A0A2S2KTU8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_16790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTU8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="63" mass="7279" checksum="4CD55CABC76E56BA" modified="2018-07-18" version="1">MLETHHKLTIIGIVLLILTFLINNYHQEVHPNDEINYAYVTGIGMLIAFSISFVMFTKDRLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTV2</accession>
    <name>A0A2S2KTV2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nucleoside diphosphate kinase</fullName>
            <shortName evidence="6">NDK</shortName>
            <shortName evidence="6">NDP kinase</shortName>
            <ecNumber evidence="6">2.7.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Nucleoside-2-P kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ndk</name>
        <name evidence="10" type="ORF">NZNM25_18470</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:18113"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7 8">Belongs to the NDK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877672.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTV2"/>
    <dbReference type="GeneID" id="76210072"/>
    <dbReference type="OrthoDB" id="6874at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04413">
        <property type="entry name" value="NDPk_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.141:FF:000018">
        <property type="entry name" value="Nucleoside diphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001908">
        <property type="entry name" value="PRK00668.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11349">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside diphosphate kinase-like" evidence="9">
        <location>
            <begin position="2"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="6 7">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH35056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="133" mass="14739" checksum="471AD18F44298B9D" modified="2018-07-18" version="1">MTEQSLFIVKPDAVARNLVGQVISRFERKGFKLLKLKMFTFTQQQAENFYGVHKDKPFFGELTSFITSGPVVAAIIEGNNAIATTRIMIGATKSFEAEPGSIRGDFGLGFSENIIHASDSQESFDHESRVAFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTV6</accession>
    <name>A0A2S2KTV6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ArnR1-like winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_18640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877688.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTV6"/>
    <dbReference type="GeneID" id="76210092"/>
    <dbReference type="OrthoDB" id="13307at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="12522" checksum="DB4802C0BDC110E0" modified="2018-07-18" version="1">MDQKINSNIKEMDIINWSVVERIMLAFYENGSLKKSQITSKSGLKYTSCMRYLKWLHTKMGFVEFELGVDLKHASSIHLSAEGILFCKKRILASEDLNSVKIQQTHLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTW6</accession>
    <name>A0A2S2KTW6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S26 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_18740</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTW6"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.109.10:FF:000033">
        <property type="entry name" value="Peptidase S26B, signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.109.10">
        <property type="entry name" value="Umud Fragment, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001733">
        <property type="entry name" value="Peptidase_S26B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02228">
        <property type="entry name" value="sigpep_I_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806:SF6">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S26" evidence="7">
        <location>
            <begin position="25"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="186"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="186"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="218"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH35083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="249" mass="28083" checksum="66F90BF298B60BFD" modified="2018-07-18" version="1">MELAKKSISKGIIKDIIIVGVGVLVIWIGLQVAFGTQNPFYVVASGSMIPVLQVYDVLIVQGHEPFEDIEVGDIIVFNRPSDHNRVIVHRVASIIDDDPKTVRTKGDANPASIPGTDFPITEEEYIGKVAYTIPQVGYVTQLLKPPINYVIIAVVIGIMVVKQMTKKKSEKEFTFTDPLDSEKTDTIEELSDIDKIEKDSENSKHMEKSKDVERLEDSEYLEEMEEVEKEMKDIDESIEDSVEKKKDRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU13</accession>
    <name>A0A2S2KU13_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3-oxoacyl-ACP reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_19230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877736.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU13"/>
    <dbReference type="GeneID" id="76210153"/>
    <dbReference type="OrthoDB" id="24596at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050259">
        <property type="entry name" value="SDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879:SF6">
        <property type="entry name" value="NADPH-DEPENDENT REDUCTASE BACG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="215" mass="24002" checksum="25F7B20670995A88" modified="2018-07-18" version="1">MTSAIILGGSRGIGKAIVESLKSIDIDVFAASKNDIDTSDLDSVRKFLGSHGKTDILVLNTGGPTPKPFHTITEDDWKLYHNQLFLGFCMILQNIKINDNGYIFLISSSVIKEPNMKLIISSAYRAAFSEVFKVLSKEYAQRNISCVNIAPGPINTDRTRELIENIEEYKKSLPMRRLGEPEEIGNFVKSIIENKIKYLSGVTINFDGANSNYIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU42</accession>
    <name>A0A2S2KU42_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CD-NTase-associated protein 12/Pycsar effector protein TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_18680</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877693.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU42"/>
    <dbReference type="GeneID" id="76210095"/>
    <dbReference type="OrthoDB" id="386561at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050135">
        <property type="term" value="F:NADP+ nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019302">
        <property type="entry name" value="CAP12/PCTIR_TIR_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10137">
        <property type="entry name" value="CAP12-PCTIR_TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CD-NTase-associated protein 12/Pycsar effector protein TIR" evidence="2">
        <location>
            <begin position="230"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="373" mass="42584" checksum="D5F1940C8B371541" modified="2018-07-18" version="1">MSEKKEKSNPKMGKRSKEHLFPRDTLKRSLNVSHAIWEQNGGNPFNILDLAPQVGYSPNSSGFRVLLASSFRYGLTEGSHITKIISLTNLGKSIVAPLEQTDVGSMLRQALLHNDLFAKVYKHFDGKPIPKKEVLRNTLVHDPSMGGFGIPREDVESFIDIFIQNIIDYNLEQDIRGTNYLRLDKLSTSNNIESEDKDMQDVEFEEEDEENKEQKPIIKEQKLSETETPKVFISHSKNKKILEQIKQMLQFGKFEYEIAIETETTAIPIPEKIFGLMRECTCAVINVSADESERKEDGSYGVNPNVLIEIGAAFLRYDKRVILLVDKKVKLPSNLQGLYQTYYEGNELSWDTAMKLQESLSKFRDNKTNAKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU48</accession>
    <name>A0A2S2KU48_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Capsular polysaccharide assembling protein CapF C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_19640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877774.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU48"/>
    <dbReference type="GeneID" id="76210194"/>
    <dbReference type="OrthoDB" id="372788at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02208">
        <property type="entry name" value="cupin_RmlC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029303">
        <property type="entry name" value="CapF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14667">
        <property type="entry name" value="Polysacc_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Capsular polysaccharide assembling protein CapF C-terminal" evidence="1">
        <location>
            <begin position="16"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="144" mass="16330" checksum="B3883A9875EF17DF" modified="2018-07-18" version="1">MKIERLLPTANLNTVRDGRGGIFTFIPKDPIVEFNFNYIKAGKIRGNHFHPEFDEYFLITEGEGVLVTKEDESEEEFIFLSKGQCTHTPKGTKHVFIAITDCIAVAMLTKKWENCQNPIIHVNMGMGKGDHGDPLSPYFKSKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU88</accession>
    <name>A0A2S2KU88_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Spore coat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_19420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU88"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02518">
        <property type="entry name" value="GT2_SpsF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003329">
        <property type="entry name" value="Cytidylyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42866">
        <property type="entry name" value="3-DEOXY-MANNO-OCTULOSONATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42866:SF1">
        <property type="entry name" value="SPORE COAT POLYSACCHARIDE BIOSYNTHESIS PROTEIN SPSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02348">
        <property type="entry name" value="CTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0167">Capsid protein</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0946">Virion</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="245" mass="28686" checksum="6C6F50D6D931495F" modified="2018-07-18" version="1">MGSSRLPGKVMRKIDNNFTVIDYVIRQLKSSKKIDKILIATTNLKEDDVICDHLFSQNVEYFRGSSDDVLDRYFQCAKKKSADIIVRITADNPLIDPNIVDSVISDYENNDCDYASNTLLRTFPYGTEVEVFSFKTLQMAWKKAKKPSEREHVTPFIYEPQNNFNLKNTKHLHNLSHLRYTVDRIEDLILVKKIIKNVHDRPILLKDILQLYKTNPNIFKINEKVKHDGYITSLKKDEQHLKRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU98</accession>
    <name>A0A2S2KU98_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acyl carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_19520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU98"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="86" mass="10085" checksum="FA04FE2B620E712F" modified="2018-07-18" version="1">MFANVMSNDKLKKMIKEIFFEIFPNLVEDDFAWGKKQNEYENWDSFAQLNLITFAEAKFNITFSLDESIEIKSANDLLKSIQSHLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUA6</accession>
    <name>A0A2S2KUA6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cobalamin biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cbiG</name>
        <name evidence="4" type="ORF">NZNM25_20130</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877897.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUA6"/>
    <dbReference type="GeneID" id="76210247"/>
    <dbReference type="OrthoDB" id="4722at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.180">
        <property type="entry name" value="CobE/GbiG C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052553">
        <property type="entry name" value="CbiG_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021745">
        <property type="entry name" value="CbiG_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021744">
        <property type="entry name" value="CbiG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002750">
        <property type="entry name" value="CobE/GbiG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036518">
        <property type="entry name" value="CobE/GbiG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038029">
        <property type="entry name" value="GbiG_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37477">
        <property type="entry name" value="COBALT-PRECORRIN-5A HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37477:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-5A HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01890">
        <property type="entry name" value="CbiG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11761">
        <property type="entry name" value="CbiG_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11760">
        <property type="entry name" value="CbiG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159672">
        <property type="entry name" value="CbiG N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159664">
        <property type="entry name" value="CobE/GbiG C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cobalamin synthesis G N-terminal" evidence="2">
        <location>
            <begin position="54"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Cobalamin biosynthesis central region" evidence="3">
        <location>
            <begin position="137"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="CobE/GbiG C-terminal" evidence="1">
        <location>
            <begin position="229"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH35222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="350" mass="38654" checksum="3EBFA8538605FE41" modified="2018-07-18" version="1">MDKTSVLAITKNGVKIGENLKKIFPDWNIFAPSKFSDEKNDIIWYHESTSEKIVELFKNSNALICLFSLGAVIRLIAPHLKDKKTDPAVIVIDDKTNFVISVLSGHIGGANELTQEIADKLQALPVITTAADVNKTIAVDLVGREFNWKIDDDATVTKISAHMVNEEPIGIFQDAGNKNWFKELPKNVSIYKNLDDLKKSNSKAYLIISDRVVDEEISKESVIYRPPSLVIGIGLHWDTTKETIKEGIENCLEKFKLSSKSIAKLVSIKKPQDVQGLIDLGKEMRIPVEYVNREDLAEISAPNPSDAVKAFEGTASVSEAAAMKVSGGDLIVEKQKFPPNLTIAIARIVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUB1</accession>
    <name>A0A2S2KUB1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribose 5-phosphate isomerase A</fullName>
            <ecNumber evidence="2">5.3.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpiA</name>
        <name evidence="3" type="ORF">NZNM25_18560</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.6" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877680.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUB1"/>
    <dbReference type="GeneID" id="76210083"/>
    <dbReference type="OrthoDB" id="19013at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004751">
        <property type="term" value="F:ribose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006014">
        <property type="term" value="P:D-ribose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01398">
        <property type="entry name" value="RPI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004788">
        <property type="entry name" value="Ribose5P_isomerase_type_A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001924">
        <property type="entry name" value="PRK00702.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00021">
        <property type="entry name" value="rpiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934:SF0">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06026">
        <property type="entry name" value="Rib_5-P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75445">
        <property type="entry name" value="D-ribose-5-phosphate isomerase (RpiA), lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="NCBIfam" id="TIGR00021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="224" mass="24403" checksum="D7D4CD7C759365C5" modified="2018-07-18" version="1">MSYDDAIAALSNDALKLVKDNYIVGLGSGRAATTLVKSLGKLIRIKNYNIRGVPTSLQIKLVAEKVGIPLVEADQVSHIDVVFDGADQIDSQKYVIKGGGGALLRENILFSLAKKVIVMADSTKFVKNFTRSVPVEVHPLARNSVISSIQKLGGKAQLRSLDRGYPFFTENGNIILDCDFGTIKNPKVITQKIKQITGVLESGIFLRKPDIIYRAKTGGKFDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUD2</accession>
    <name>A0A2S2KUD2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">4-hydroxybutyryl-CoA dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">abfD</name>
        <name evidence="7" type="ORF">NZNM25_18760</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877699.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUD2"/>
    <dbReference type="GeneID" id="76210103"/>
    <dbReference type="OrthoDB" id="32865at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3140.10">
        <property type="entry name" value="4-hydroxybutyryl-coa dehydratase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004925">
        <property type="entry name" value="HpaB/PvcC/4-BUDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024719">
        <property type="entry name" value="HpaB/PvcC/4-BUDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024674">
        <property type="entry name" value="HpaB/PvcC/4-BUDH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36117">
        <property type="entry name" value="4-HYDROXYPHENYLACETATE 3-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36117:SF3">
        <property type="entry name" value="4-HYDROXYPHENYLACETATE 3-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03241">
        <property type="entry name" value="HpaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11794">
        <property type="entry name" value="HpaB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000331">
        <property type="entry name" value="HpaA_HpaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HpaB/PvcC/4-BUDH N-terminal" evidence="6">
        <location>
            <begin position="10"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="HpaB/PvcC/4-BUDH C-terminal" evidence="5">
        <location>
            <begin position="285"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH35085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="508" mass="56929" checksum="CF1D250C625382DA" modified="2018-07-18" version="1">MANVLKTIRTGDDYIESLRGRDLKIYLFGELVKEPVDHPMIRPSINAVAETYDLAVREEELASADSSLTGLRVNRFLHIAESAQDLVLQNKMQRKLGQNTGTCFQRCVGMDALNSLHSTTFEIDEKHGTNYHERFLEFVKMVQKENLVIGGAMTDPKGDRSKGPAEQDDPDLFTRIVDSDEKGVYVSGAKAHQTGCINSHWIILMPTIRLTENDKDWAIVGAIPADAKGVTYIYGRQSCDTRSMEEGDIDDGNSKFGGQEALIILDRVFIPWDKVFMNGEFEFASMLIERFTCYHRRSYVCKTGLGDVLIGAAATIADYNGVPKVSHIKDKIIEMTHLNETIFAAGIASSHQGYKMKSGVYLNDDMLAQVCKHNVTRFPYEISRLAQDIAGGLVVTLPSEKDFRHPEAGPLLKKYLAGRKGVDVENRMRILRLIENMTLGRNAVGYLTESMHGAGSPQAQRIQIQRQMQIGYKKNLAKNLAGIKNDVEEPHEPSEYFKRVFKTKDSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNM4</accession>
    <name>A0A2S2KNM4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_00040</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutaredoxin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioredoxin family. DsbA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875889.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNM4"/>
    <dbReference type="GeneID" id="76209329"/>
    <dbReference type="OrthoDB" id="15256at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13887:SF14">
        <property type="entry name" value="DISULFIDE BOND FORMATION PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13887">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13462">
        <property type="entry name" value="Thioredoxin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="31"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="234" mass="25801" checksum="425E8CC6F1FDDE85" modified="2018-07-18" version="1">MIHGPSLGIGAGIASIVIIVIFLGFQGSQPELTIEPAPEIQESGSSKITMNTFLANGSPILGNPNAPVTLVEFGDYQCHFCNVFFHSTEDQILENYVKTGKVRMIFKDYNIIGPDSVTASHGAHCADDQELFWEYHDILYSNWTGENNGWASSENLVKFAQEVGLDLDEWSECMNTQKHAQIILTSNEDAKSLELTGTPAFFVIGPDGKTTRIFGAQPFEVFENIFETELGKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNN1</accession>
    <name>A0A2S2KNN1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl24</name>
        <name evidence="7" type="ORF">NZNM25_00310</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Located at the polypeptide exit tunnel on the outside of the subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="4">One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875913.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNN1"/>
    <dbReference type="GeneID" id="76209357"/>
    <dbReference type="OrthoDB" id="10899at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01326_A">
        <property type="entry name" value="Ribosomal_uL24_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_uL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005756">
        <property type="entry name" value="Ribosomal_uL24_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="Ribosomal_uL24_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01080">
        <property type="entry name" value="rplX_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11143">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L26 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16906">
        <property type="entry name" value="Ribosomal_L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01108">
        <property type="entry name" value="RIBOSOMAL_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="KOW" evidence="6">
        <location>
            <begin position="44"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="112"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="150" mass="17139" checksum="6FA6E7F3F26874CC" modified="2018-07-18" version="1">MKPTKMRNKMMYQATFQTRSKQLGSALSKELRKKYGKRSVRAIEGDSVTILRGEFKGVEGKISKVSTEKSSVAIEGVKKEKTKGDKFDVYIHTSNLVVTSLSTDDRWRISKLEGKDPRKQPKETQVKDKPVEEETKETKVANKEKKEDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNQ1</accession>
    <name>A0A2S2KNQ1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00710</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875948.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNQ1"/>
    <dbReference type="GeneID" id="76209400"/>
    <dbReference type="OrthoDB" id="4867at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="188" mass="22085" checksum="EF860FA7FCE04731" modified="2018-07-18" version="1">MEKDSQIIRTEIIRILNENGKIRGTELAKRVIEKMGNEKTVYREISALVEAGEIEKKVHSRSHIEYELINLYESVNNQLKSLHKEIETVFEEIKNFTIISKEENFSFHERLRSVIHLIHIVQSTDGIMKLLSHYPAFKKDKMFSQINRKIDDCWKIIMDVIAHQPEKDFLNEVLANLRIPQIDTSNVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNU1</accession>
    <name>A0A2S2KNU1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_00730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875950.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNU1"/>
    <dbReference type="GeneID" id="76209402"/>
    <dbReference type="OrthoDB" id="2795at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="20"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="77" mass="9312" checksum="5C8C91D5A66AEEF3" modified="2018-07-18" version="1">MDEKKEDKSAESMKNHIVYYRSLTKVISNIKKEKEQENESAIKEHLENRIEAMEKDRKRIRDMFPEIKEEEWNGNAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNU5</accession>
    <name>A0A2S2KNU5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01310</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNU5"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="72" mass="8096" checksum="83EF57BC8F139AD6" modified="2018-07-18" version="1">MFENLIKIVVNGMNMTDDKNEIEKLIDTMISSGDDLVGNLKNILPDSLAESVVMFHESNVSNLKKIRAFLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNV8</accession>
    <name>A0A2S2KNV8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01540</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_160049182.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNV8"/>
    <dbReference type="GeneID" id="76209483"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="51" mass="5789" checksum="C6D82F281E0BE720" modified="2018-07-18" version="1">MSQSIRAVVSKKNYDKIIKLTELPFDIGFDKAITLILDKYEKSITKDGAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNX6</accession>
    <name>A0A2S2KNX6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Peptide transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_01230</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875997.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNX6"/>
    <dbReference type="GeneID" id="76209452"/>
    <dbReference type="OrthoDB" id="312811at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43839">
        <property type="entry name" value="OPPC IN A BINDING PROTEIN-DEPENDENT TRANSPORT SYSTEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43839:SF1">
        <property type="entry name" value="OPPC IN A BINDING PROTEIN-DEPENDENT TRANSPORT SYSTEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="253"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="368"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="420"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="251"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="455" mass="50257" checksum="05563B22DF34D878" modified="2018-07-18" version="1">MSGITLQEIKQEFLKSNMGIAGISILIILIGISIIAMIAIPIETFQEWNNPGSWILYPKVAIPVWMNLFMTEKIPEHKILENPSVQNILQDEIILTSHQFGLNFDYDYFPNDFIYVFSSEYVGSPLLKMSVVRPDGIQLELLSTALPHANSKTVHSERIFSTDDAIKKNLSLQSEKFQFSLDGLSSEDIVFSKIETNEPLKGKYVFSMNLYGVNSENQIHESKLIIGGKAFGVMGTDELRRDLAVGLLWGTPLALFIGIVVSIASVVMGLLYGVYAGFKGKKTDEAMMRFNDVIYALPALPFLIILSVTISNSIFLMIGFLMIFGWVGIAKVARSMSLQIKTRGYVDAANMMGQKDSKIIFKHILPQLLPYAFASIAISVPAAITTEAGLSFLGLGDPSFPTWGQILHDANTFGAAARGLWWWIMPPGVMIAITGLAFVFIGNALDAIVNPKLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNY0</accession>
    <name>A0A2S2KNY0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">tRNA (Adenine-N1)-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_01710</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876039.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNY0"/>
    <dbReference type="GeneID" id="76209501"/>
    <dbReference type="OrthoDB" id="30774at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160107">
        <property type="term" value="F:tRNA (adenine(58)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049470">
        <property type="entry name" value="TRM61_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014816">
        <property type="entry name" value="tRNA_MeTrfase_Gcd14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133:SF2">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE CATALYTIC SUBUNIT TRMT61A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08704">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017269">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51620">
        <property type="entry name" value="SAM_TRM61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61 C-terminal" evidence="7">
        <location>
            <begin position="57"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="264"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="264"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="278"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="295"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="107"/>
            <end position="110"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR017269-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="305" mass="34446" checksum="DE7779945FCB1CA9" modified="2018-07-18" version="1">MAKIKQNSPVLFFYNNSKKWLAKISKKESFHTHIGVIKHSDAIGKEYGSRLTTNKDKYVYLLEPTMYDYVMKIQHGTQIVYPKDLGYIVARSGIGDGQKILEIGTGSGSLTSFVANIVKPRGHVYTFDVDENFMKIAEKNIKKAGVSKYVTQQNLDLKTAKTLPLSDMDVALIDLGDPWIVIPKVRQMLKGSGCIFAICPTMNQLEKLTTALVENEFTDIESTEHIIRTIEAREGKTRHSFQGIGHTTYLCFARKAFFDRQLRKSPEEPPIDKIPSKTPKKTLKKTTRTKKSTKKSLGSTHKTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP03</accession>
    <name>A0A2S2KP03_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">AsnC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_00980</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875973.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP03"/>
    <dbReference type="GeneID" id="76209426"/>
    <dbReference type="OrthoDB" id="6762at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154">
        <property type="entry name" value="LEUCINE-RESPONSIVE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR AZLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13404">
        <property type="entry name" value="HTH_AsnC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="3"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="147" mass="16550" checksum="918D55633356A3BB" modified="2018-07-18" version="1">MNLDSTDEKILKNLMMDARQSARQLALKLGMSTVTVLSRIKKLEKEKIIKGYTATIDHEKIGYSLTAIIEIVAKNDKIVDMEEEISKFENVCGVYDITGSTDTIIIAKFKERNELSKFVKGLATIPNVENTITHVVLNTAKEDFRLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP09</accession>
    <name>A0A2S2KP09_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Phosphoglycolate phosphatase</fullName>
            <shortName evidence="5">PGP</shortName>
            <shortName evidence="5">PGPase</shortName>
            <ecNumber evidence="5 6">3.1.3.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_01770</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the dephosphorylation of 2-phosphoglycolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>2-phosphoglycolate + H2O = glycolate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14369"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58033"/>
            <dbReference type="EC" id="3.1.3.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal SPP-like hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.18" evidence="5 6"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876045.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP09"/>
    <dbReference type="GeneID" id="76209509"/>
    <dbReference type="OrthoDB" id="120822at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07514">
        <property type="entry name" value="HAD_Pase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01419">
        <property type="entry name" value="GPH_hydrolase_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006382">
        <property type="entry name" value="PGPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01487">
        <property type="entry name" value="Pglycolate_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01482">
        <property type="entry name" value="SPP-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10000:SF8">
        <property type="entry name" value="HAD SUPERFAMILY HYDROLASE-LIKE, TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10000">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="NCBIfam" id="TIGR01487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="231" mass="24906" checksum="E7F26BFD2F6ABEBC" modified="2018-07-18" version="1">MKKRTFAVDIDGTITENGGGRIHLDALDALRRLTNMGHTVIFVTGRSSVEGYLLSVFGGTTKIAVGENGGCITLDSNDHILLGNIEECKTALNVMKNSIDNVKEKLVFPRMTEVVLERTFDLDLARKLLSENNIDVELSDSQYAFHINSSGVDKGTGFSKIMEKFSILRDDVIAIGDSATDVPLFRLAKTSVALGNASEIVKSEATMTVSANAGDGVLEALDKLAPRLSEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP10</accession>
    <name>A0A2S2KP10_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_02140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876082.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP10"/>
    <dbReference type="GeneID" id="76209548"/>
    <dbReference type="OrthoDB" id="382495at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="130" mass="15157" checksum="3BBF8E4A3327F961" modified="2018-07-18" version="1">MKTRFKIPSYKTNILFMLNAVIVGILSIGASYFTLGPLIMFERNISDIVRMVTQNYAMIGLWIIYFANIICLPINLKKTHKNWKLGYIVFLISTMIFVIYITGVFDCWGAMISRFFGMDYNDRIRCLNEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP33</accession>
    <name>A0A2S2KP33_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF5615 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_00720</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875949.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP33"/>
    <dbReference type="GeneID" id="76209401"/>
    <dbReference type="OrthoDB" id="7785at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041049">
        <property type="entry name" value="DUF5615"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18480">
        <property type="entry name" value="DUF5615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5615" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="91" mass="10580" checksum="799F791BC2A74F32" modified="2018-07-18" version="1">MKILVDENLDGMDERLINLGYDAQSVRKLQREGKKLGSDYSIINYARENDMIIVTKDTEFRKASDENNFPLILLDDEEILKVIVEKLKNFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP44</accession>
    <name>A0A2S2KP44_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ArnR1-like winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_01380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876009.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP44"/>
    <dbReference type="GeneID" id="76209467"/>
    <dbReference type="OrthoDB" id="373882at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038723">
        <property type="entry name" value="ArnR1-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14947">
        <property type="entry name" value="HTH_45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ArnR1-like winged helix-turn-helix" evidence="1">
        <location>
            <begin position="11"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="98" mass="11397" checksum="50C586164365C863" modified="2018-07-18" version="1">MMAKKKLQRFDLKVLLRLLAAVYESGHEKRTNLALNARLHYDTCVSYLDMLEFLGFVTKSNRYSHQVYDVTPHGISLCKKKLDAEFDKKYNGILTILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP46</accession>
    <name>A0A2S2KP46_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_02030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876071.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP46"/>
    <dbReference type="GeneID" id="76209537"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="66" mass="7760" checksum="38994DD8FB36CE1B" modified="2018-07-18" version="1">MAKVIKQYSFKALMLLEYLSVILYIMSFTLVPFIAVLGISLRVIKKKEQTRTYRKPQTELEEIMGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP60</accession>
    <name>A0A2S2KP60_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sjogrens syndrome scleroderma autoantigen 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_02640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876123.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP60"/>
    <dbReference type="GeneID" id="76209599"/>
    <dbReference type="OrthoDB" id="26305at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009563">
        <property type="entry name" value="SSSCA1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06677">
        <property type="entry name" value="Auto_anti-p27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="12301" checksum="7B5FBED54E3BC209" modified="2018-07-18" version="1">MPEDLTKKAAEMLLNGATLLGEPCPYCSGVRVMKEGHALCVSCGREPEKKEIMTEAPQHPKSRLEETLEEKIELLSKELEEEKNHDKQQSILKSINSVLETLGKIREKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPB7</accession>
    <name>A0A2S2KPB7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_02250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876091.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPB7"/>
    <dbReference type="GeneID" id="76209559"/>
    <dbReference type="OrthoDB" id="2255at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="157" mass="18926" checksum="05D179024F1FCA97" modified="2018-07-18" version="1">MSENLENIKKSIREHSEKLAKLGTELAEIKFSYKVEEKPSKEYWEKRMNEYKKYNEKCLEYYNQVHSMINLINKEKSQMFLLHISKFRQLGLELLEIMQKIKDNPSIADPKDKQKSQWSKDIKNKVSEQSNKCLNHEKEMNGSFRNFYQNELKKILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPC8</accession>
    <name>A0A2S2KPC8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ferritin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ftnA</name>
        <name evidence="6" type="ORF">NZNM25_03210</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876271.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPC8"/>
    <dbReference type="GeneID" id="76209659"/>
    <dbReference type="OrthoDB" id="4859at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01055">
        <property type="entry name" value="Nonheme_Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.10:FF:000001">
        <property type="entry name" value="Non-heme ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001519">
        <property type="entry name" value="Ferritin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041719">
        <property type="entry name" value="Ferritin_prok"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431:SF127">
        <property type="entry name" value="BACTERIAL NON-HEME FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0409">Iron storage</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="5">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="172" mass="19205" checksum="4D6AE7F197361ACF" modified="2018-07-18" version="1">MKLSPKMKKALDDQVTLEASASNSYLAMASWCEVTGYQGAANYFYAQSDEERTHMLKLVHFLNALGAVATIPAIKAPVSSYKSLEGLIKSALKNEQIVTAAIHKMVEIAQKEKDHSTYAFLEWFVNEQVQEETKFETILQKFDLLGRDKLGINEIDKYLATQAAKPQTDPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPF9</accession>
    <name>A0A2S2KPF9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_03540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876202.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPF9"/>
    <dbReference type="GeneID" id="76209694"/>
    <dbReference type="OrthoDB" id="27639at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="134" mass="15774" checksum="FF9E4200A11C8268" modified="2018-07-18" version="1">MRSTKIVTSFIRNNEKLLILKRSDKVKSMKGLWAGISGIIENNEEPLKRAKIEIFEEVGITEDKITLVKSAEGMRVNSPQYENHEWEIYPFLFEVENTEIELNWENSEFEWIDIKELENYETVPSLQQVLLNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPK7</accession>
    <name>A0A2S2KPK7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CAAX prenyl protease 2/Lysostaphin resistance protein A-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_02620</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876121.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPK7"/>
    <dbReference type="GeneID" id="76209597"/>
    <dbReference type="OrthoDB" id="10162at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080120">
        <property type="term" value="P:CAAX-box protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="2">
        <location>
            <begin position="158"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="329" mass="37085" checksum="4A439EB1E8E99EF7" modified="2018-07-18" version="1">MQNSNRILQFLGIPFTALQSVILGLLLVSFPIGIYIVFESDIGGDINHEYPLTHLTLFEGTELYQFPLDVSIGDVFAVLWTFYAILFAIAIFGPKDGFLKSISSIISFGKFNTTSNYMIGITKWFSILILISALINFIQEAFGIVTVPPLDDNDLIQFFYVSLAPFVEEFGFRLILIGIPLFVLYSHKSSLRYFVKCLWTPSSLHLHDNKKAILLIVFVGVFFGFAHIAFAESWSEGKFAQAAASGIILGWVYLRYGFVASLLIHWAMNYFIFSYANFISQLNFISVEEAFSHSLMSSLEMLLLISGIISTSILFVNRFHSKKESALEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPK9</accession>
    <name>A0A2S2KPK9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Cysteine desulfurase</fullName>
            <ecNumber evidence="3 8">2.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">csdB</name>
        <name evidence="10" type="ORF">NZNM25_04010</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L-selenocystine to produce L-alanine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>(sulfur carrier)-H + L-cysteine = (sulfur carrier)-SH + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:43892"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="2.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="3 8"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876283.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPK9"/>
    <dbReference type="GeneID" id="76209747"/>
    <dbReference type="OrthoDB" id="5817at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006534">
        <property type="term" value="P:cysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06453">
        <property type="entry name" value="SufS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010970">
        <property type="entry name" value="Cys_dSase_SufS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016454">
        <property type="entry name" value="Cysteine_dSase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01979">
        <property type="entry name" value="sufS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586:SF8">
        <property type="entry name" value="CYSTEINE DESULFURASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005572">
        <property type="entry name" value="NifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="9">
        <location>
            <begin position="29"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH33610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="414" mass="46590" checksum="7B54E94B072B5BCC" modified="2018-07-18" version="1">MQSTESLFENIRKDFPILERTVRDNKQLVYLDNASTTQKPNQVIDAITDYYQHHNANIHRAVYALAEEATEAYEATRDKIANFINIKDRQEIIFVRGTTEAINLVAYSWGRPHIKEGDIIVTTEYEHHSNIVPWQLLTQEKHAKLEYIGMDDNGELILDDLDKYLATGKVKLVTFSLMSNVLGTITDYEKIIEKCKAAGVLTLIDGAQAVPHMKVDIEKLGCDFFAFSGHKMLGPTGIGVLWVRKSVLDTMVPFHGGGDMIREVHKYETTWNDLPYKFEAGTPNIADVVGFGAAIDYLTKIGMDNIREHEIDLTKYALEKLSAVKGLHIYGTKDMSKRGGVISFNFADVHPHDVAQIIDEEGIAVRSGHHCAQVLMERLNVAATSRASFYIYNTKEDVDILVNSLNIVAKVFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPL7</accession>
    <name>A0A2S2KPL7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Arsenate reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_04110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876542.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPL7"/>
    <dbReference type="GeneID" id="76209758"/>
    <dbReference type="OrthoDB" id="295776at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046685">
        <property type="term" value="P:response to arsenic-containing substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16345">
        <property type="entry name" value="LMWP_ArsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023485">
        <property type="entry name" value="Ptyr_pPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036196">
        <property type="entry name" value="Ptyr_pPase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43428">
        <property type="entry name" value="ARSENATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43428:SF1">
        <property type="entry name" value="ARSENATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01451">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00226">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52788">
        <property type="entry name" value="Phosphotyrosine protein phosphatases I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0059">Arsenical resistance</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphotyrosine protein phosphatase I" evidence="2">
        <location>
            <begin position="5"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="134" mass="15199" checksum="138C4283F040A8B6" modified="2018-07-18" version="1">MAKPEKVLFVCVENAGRSQMAEGFLRKFAPHLDVISAGTKPRSQLIPTVIDVMKEVGIDITEQKPKELTNEMISQSITVNMGCMDKESCPALFVNDVIDWNISDPKDKDIEKIREIRDQIKNEVLNLIKKIEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPT4</accession>
    <name>A0A2S2KPT4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Roadblock/LAMTOR2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876181.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPT4"/>
    <dbReference type="GeneID" id="76209670"/>
    <dbReference type="OrthoDB" id="1734at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046600">
        <property type="entry name" value="DUF6659"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20364">
        <property type="entry name" value="DUF6659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="13686" checksum="626AA4A95A67879B" modified="2018-07-18" version="1">MNSEEDLDQQRCHKILELPEIRFAGFLDYMGNLIVGDFKSGIIPLKNENERKKMFMEAVLRIRTRQEFDENLGQVEYAAARRKNVVTMTIPLEKKILFIAAESNADIDKTARKIIDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPY3</accession>
    <name>A0A2S2KPY3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_05410</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the transposition of the insertion sequence.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866823.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPY3"/>
    <dbReference type="GeneID" id="76209893"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032874">
        <property type="entry name" value="DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052183">
        <property type="entry name" value="IS_Transposase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047930">
        <property type="entry name" value="Transpos_IS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033587">
        <property type="entry name" value="transpos_IS6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35528">
        <property type="entry name" value="BLL1675 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35528:SF3">
        <property type="entry name" value="BLL1675 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13610">
        <property type="entry name" value="DDE_Tnp_IS240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0815">Transposition</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="6">
        <location>
            <begin position="41"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="7">
        <location>
            <begin position="193"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="374" mass="43516" checksum="5C68556B21736C9E" modified="2018-07-18" version="1">MTTQTNPRQEKGKVIAEQGNQIRRIDESTYSVRSQTRHVNYTVTKTNSGFVCNCPDHIFRKLCCKHIHSVEISIKLREQVREQNKVTIEPINIQICQFCNSDNLKKSGIRHNKSGDIQRFACKDCKKTFSINLGFEKMKSSPEIITQSLQLYFTGESLRNVQKFLKLQGVNVSHKTIYGWVVKYTKLMKSYLDKITPSVGDTWRADEVYVKIRGELKYVFSLMDDQTKFWIAQEVSHRKQGHNARGLLQQAKQVTKITPKVFITDGLASYSEAYKREFWARERSQRTTHIRHIHITGDKNNNTMERLNGEFRDREKVTRGLKKMDSPLIDGYQLYHNYVRPHMSLEGKTPSEMCGIEVKGNDKWKTLIQNASKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ07</accession>
    <name>A0A2S2KQ07_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03920</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876237.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ07"/>
    <dbReference type="GeneID" id="76209734"/>
    <dbReference type="OrthoDB" id="6477at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="103" mass="12736" checksum="33CCD990A79F8666" modified="2018-07-18" version="1">MRNDERFEIQRAFDLLPHVIGSSWAVIWFRMKGIKKPTRDEYREKTLEFLRKIEPVFESYPKEEGFLDIIKYIEFRKNEEYEKIRSGKNKEVETRYDRYVDYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ43</accession>
    <name>A0A2S2KQ43_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_05600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866824.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ43"/>
    <dbReference type="GeneID" id="76209911"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050407">
        <property type="entry name" value="Geranylgeranyl_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685:SF21">
        <property type="entry name" value="DEHYDROGENASE (FLAVOPROTEIN)-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685">
        <property type="entry name" value="GERANYLGERANYL DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="355" mass="40866" checksum="7B564EA07BAFE5D1" modified="2018-07-18" version="1">MLNFQIVYLLGIQRSQDILKIAVMGMGVAGSYLMARLKDSEHEVVGYERSTEDRHDSICAWGTIKPVLTDFCKKTGRDFNDFLIHDGKNMHVKMNNDVKFDIGLKGLCTYNKLGLIKDFIKDSKIIYGESPKLEELEKEYDMIVDCTGFNRVYLPKLKEDFFLPTYEYKVEYENGVPYDDFYIEPFPGMSGYFWYFPLGEKWAHIGAGDYNKNHIKATDEFLQKHGGKVIATKGRPIRLATPDRCKPYYSGKVVGVGESIGTVYALLGEGIIPSMQCVEIFLENMNDFKAYEKAVEQHYKVYAKVFNFVRAKIHKDFSFLKALPDFIAIFRYMKKNEDRFGMNIKIADLLKVAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ48</accession>
    <name>A0A2S2KQ48_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Translation initiation factor 5A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Hypusine-containing protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 11">eIF-5A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">eif5a</name>
        <name evidence="13" type="ORF">NZNM25_03690</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Functions by promoting the formation of the first peptide bond.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the eIF-5A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876215.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ48"/>
    <dbReference type="GeneID" id="76209710"/>
    <dbReference type="OrthoDB" id="23689at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045901">
        <property type="term" value="P:positive regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045905">
        <property type="term" value="P:positive regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04467">
        <property type="entry name" value="S1_aIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000038">
        <property type="entry name" value="Translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00085">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001884">
        <property type="entry name" value="IF5A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048670">
        <property type="entry name" value="IF5A-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020189">
        <property type="entry name" value="IF5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019769">
        <property type="entry name" value="Trans_elong_IF5A_hypusine_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022847">
        <property type="entry name" value="Transl_elong_IF5A_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00037">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003076">
        <property type="entry name" value="PRK03999.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11673">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 5A FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01287">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21485">
        <property type="entry name" value="IF5A-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003025">
        <property type="entry name" value="eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01376">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00302">
        <property type="entry name" value="IF5A_HYPUSINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11" id="KW-0385">Hypusine</keyword>
    <keyword evidence="6 11 13" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation initiation factor 5A C-terminal" evidence="12">
        <location>
            <begin position="74"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="modified residue" description="Hypusine" evidence="11">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH33578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="136" mass="14965" checksum="412279423AF1FCD1" modified="2018-07-18" version="1">MSKPSDLGSLKIGSYILLPHTDQPSGEPCRIVEYDTSKPGKHGAAKARIVGEGIFDGQKRPLVGPVSMQIHVPMINKKVGQIISINGDTVQVMDSETFETIDISLIDDEVKGKLENGQNVEYWVVMDKTKIMRIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ59</accession>
    <name>A0A2S2KQ59_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">eRF1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_04780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876347.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ59"/>
    <dbReference type="GeneID" id="76209830"/>
    <dbReference type="OrthoDB" id="1011at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004403">
        <property type="entry name" value="Peptide_chain-rel_eRF1/aRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10113">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1" evidence="1">
        <location>
            <begin position="133"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="351" mass="40443" checksum="93592C9CFE7A9806" modified="2018-07-18" version="1">MKNNSNTGISPSEWLSINRFLYEVKQIDTLCVSVYYPYGKGQETMSLLEETKRNEFFEKIESKIEQKIADLKDNPISVGKFAKTLCIFGWIKNNKVNIKVIGTSKKLPYVYMASKKPFIKPFKDILKTNYDVLLVTLDQKSARIQKFHGSKIVLESKLRIDLQGRHRKGGQSQGRFLRARQTKIHVFFKKIAKRLCEVDLNSDLILLGGNGQAKTEFYDELDSELAKKCVFVENLSFSTSINDIEKKIIHHLYQHRRKYVAEIIKKYESLVKKGLTAKRNDVIFKALETGAVDTLIVSANYYSDSQFKKIMKMLELAKNTSCKIEFASSPKIIQKLELDNSVIAILRYKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ85</accession>
    <name>A0A2S2KQ85_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">uroporphyrinogen-III C-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_04060</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.107" evidence="1"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876541.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ85"/>
    <dbReference type="GeneID" id="76209752"/>
    <dbReference type="OrthoDB" id="24444at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004851">
        <property type="term" value="F:uroporphyrin-III C-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11642">
        <property type="entry name" value="SUMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.950.10:FF:000001">
        <property type="entry name" value="Siroheme synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1010.10:FF:000001">
        <property type="entry name" value="Siroheme synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006366">
        <property type="entry name" value="CobA/CysG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050161">
        <property type="entry name" value="Siro_Cobalamin_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01469">
        <property type="entry name" value="cobA_cysG_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004790">
        <property type="entry name" value="PRK06136.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790:SF3">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT UROPORPHYRINOGEN III METHYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790">
        <property type="entry name" value="SIROHEME SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00840">
        <property type="entry name" value="SUMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="7">
        <location>
            <begin position="4"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="246" mass="26811" checksum="9B991C327E4D388A" modified="2018-07-18" version="1">MTGKVYLVGAGPGDHKLITLRAVELLKKADVVLYDRLVSKKIISMIPKSAKKVYVGRAVGDDTTHQNTTNDLMVKYAKSKKNVVRLKGGDPIIFGRGGEEAEFLKENKVKYEIVPGITSGIGSATYAGIPLTHRKYASSVVFVTGHEDPEKKKEIVKWKRLAKSVDTIVIMMGLSRIDVICKQLIAGGMNTKTPVAVIQNGTTPNQKMIKGTVTNIAKKVKENKITPPTNIIIGNVVNLSESIGWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQA8</accession>
    <name>A0A2S2KQA8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_05850</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQA8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1067" mass="114480" checksum="E07ED6A36DBAC5A5" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQB0</accession>
    <name>A0A2S2KQB0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_06610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876500.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQB0"/>
    <dbReference type="GeneID" id="76210018"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="100" mass="10766" checksum="4BC86C5630402ED8" modified="2018-07-18" version="1">MSAINNRSDKKSSKNTTFIIAGVIALGSSLLFAYLMFYTAPEHNMEMVKVIANTESGCIAETMDGFAVNIGACNAEPGKYVNALVDQKTKERAALMNPTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQD2</accession>
    <name>A0A2S2KQD2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytidyltransferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_06730</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876511.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQD2"/>
    <dbReference type="GeneID" id="76210033"/>
    <dbReference type="OrthoDB" id="7814at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="23420" checksum="A610DD23256B7110" modified="2018-07-18" version="1">MITALYLAHLNPVTNAHVEIISDLKREADTVKVMPVVFRDGDKEINSKSFPFNFEIRKKMLLSVFGDSIQITDDYAFFAPFKKYMPPLLAPKSWQLRKQILRGVQGDFFSYTGDKAEGYMLKIYRLNPKVGERKSLSAASVKEKLYDAALGKESSWKEYVPESIAKIIEEEWETVERFSNSEDLTTRVVGMKFPKEGWSKNSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQF6</accession>
    <name>A0A2S2KQF6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="12">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Alanyl-tRNA synthetase</fullName>
            <shortName evidence="12">AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">alaS</name>
        <name evidence="15" type="ORF">NZNM25_05520</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Ala) + L-alanine + ATP = L-alanyl-tRNA(Ala) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="12"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876410.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQF6"/>
    <dbReference type="GeneID" id="76209902"/>
    <dbReference type="OrthoDB" id="7506at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.310.40:FF:000001">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.54.20:FF:000008">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000004">
        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.54.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_A">
        <property type="entry name" value="Ala_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022429">
        <property type="entry name" value="Ala-tRNA_lgiase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050058">
        <property type="entry name" value="Ala-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03683">
        <property type="entry name" value="A-tRNA_syn_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777:SF9">
        <property type="entry name" value="ALANINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777">
        <property type="entry name" value="ALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 12 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alanyl-transfer RNA synthetases family profile" evidence="14">
        <location>
            <begin position="70"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="740"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="587"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="591"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="691"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="695"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GBH33761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="889" mass="100090" checksum="5EEE363B7B677DBE" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQI6</accession>
    <name>A0A2S2KQI6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Radical SAM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_05820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829042.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQI6"/>
    <dbReference type="GeneID" id="76209934"/>
    <dbReference type="OrthoDB" id="358785at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.280">
        <property type="entry name" value="Cobalamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42731:SF4">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42731">
        <property type="entry name" value="SLL1084 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Elp3/MiaA/NifB-like radical SAM core" evidence="1">
        <location>
            <begin position="214"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="526" mass="59461" checksum="E2ABA6745F30D1B5" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQN7</accession>
    <name>A0A2S2KQN7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Reductase DrgA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">drga</name>
        <name evidence="4" type="ORF">NZNM25_05590</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the nitroreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876415.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQN7"/>
    <dbReference type="GeneID" id="76209910"/>
    <dbReference type="OrthoDB" id="287850at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02137">
        <property type="entry name" value="MhqN-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="entry name" value="NADH Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43673">
        <property type="entry name" value="NAD(P)H NITROREDUCTASE YDGI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43673:SF12">
        <property type="entry name" value="PROTEIN DRGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="FMN-dependent nitroreductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="3">
        <location>
            <begin position="7"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="199" mass="22401" checksum="B54C86759E5E3FDB" modified="2018-07-18" version="1">MDTFDAIKSRRSIKSFKNHEMTEDEINQLLEAAILSPTSYNIQNWRFVIVTEQKLKDRISELSYGQRQVAEASLVIVLCADLKAWDRNPERYWKNVPEESRNYLVSGIRQAYSGNETLEKEQAIRSCGIAAQTIMLAAKSMGYDSCPMEGFDYGKVGNLINLPDDHIVTMMIVVGKAAKEAAPRGGQIPLSDVVFENSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQR4</accession>
    <name>A0A2S2KQR4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 2">Argininosuccinate lyase</fullName>
            <shortName evidence="1">ASAL</shortName>
            <ecNumber evidence="1 2">4.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Arginosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argH</name>
        <name evidence="5" type="ORF">NZNM25_06620</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-(N(omega)-L-arginino)succinate = fumarate + L-arginine</text>
            <dbReference type="Rhea" id="RHEA:24020"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="EC" id="4.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.1" evidence="1 2"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876501.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQR4"/>
    <dbReference type="GeneID" id="76210021"/>
    <dbReference type="OrthoDB" id="27337at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00114"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.40.30:FF:000001">
        <property type="entry name" value="Argininosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000015">
        <property type="entry name" value="argininosuccinate lyase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00006">
        <property type="entry name" value="Arg_succ_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029419">
        <property type="entry name" value="Arg_succ_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814:SF1">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14698">
        <property type="entry name" value="ASL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00145">
        <property type="entry name" value="ARGSUCLYASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarate lyase N-terminal" evidence="3">
        <location>
            <begin position="21"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Argininosuccinate lyase C-terminal" evidence="4">
        <location>
            <begin position="360"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="NCBIfam" id="TIGR00838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="486" mass="54068" checksum="2590F3E1921942A2" modified="2018-07-18" version="1">MYRSRLGTDLSDITLDYVSSINDDSEIAYYDIIGSQAHTLMLYQNNIITKNDAKKILSALNFLKNEKFDVSSGAEDIHELIESLVIKKAGMASGGKMHTARSRNDQVSLDIRMKIRDDINIICSCLLDAIEALVSVAKNHQKTIMPLYTHLQQAQAGLFSHYLLAHADVLSRDFQRLYGTFERVNQSPLGAGPVGGTSIPIDRHSTAKMLGFDDVIENSIDATSTRDFVAEYIAMISILMTNLSKMSEDFVIWSTSEFSFIELADEFTSPSSVMPQKKNPDILELTRGKTSEIIGNLTAILTTIKGLASGYGRDLQQIKSSIWSTSKISISALLILKSILLTLKVNEKQMKKVTESSNLIALDIAEKLVKEGIPFRVTHQISGSLVQMAYQSKKPISKLTPDQIKKSVSDTKVDPKLVSKIIANTTVVSSLKDRKSFGSSGYDEQKRMISDRTHKINQYRIDVTKRENKISSAIESLTKQINEIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQT3</accession>
    <name>A0A2S2KQT3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GINS subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_06820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876520.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQT3"/>
    <dbReference type="GeneID" id="76210042"/>
    <dbReference type="OrthoDB" id="8637at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11714">
        <property type="entry name" value="GINS_A_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038437">
        <property type="entry name" value="GINS_Psf3_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GINS subunit" evidence="1">
        <location>
            <begin position="70"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="173" mass="20097" checksum="814AF530E3F47E81" modified="2018-07-18" version="1">MEIDKVEKVHSIGYRLKDAKFTVNQDFKFNVAGVKIDATQGDTDNIPRWVGKILSDNNLGVLDSPDMITELKQALSKEKMVGEYQISTLDPHFYIKLRESMKELNRDDFDKVESMMLELFRMRRGKLVKTADSIKLNSELYNKLTVEENIFYQTIYDNSKEFEKQITGGLNEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQU5</accession>
    <name>A0A2S2KQU5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADH:ubiquinone reductase (non-electrogenic)</fullName>
            <ecNumber evidence="2">1.6.5.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">NZNM25_07280</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a quinone + NADH + H(+) = a quinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:46160"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.9" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQU5"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) (non-electrogenic) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054585">
        <property type="entry name" value="NDH2-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706:SF47">
        <property type="entry name" value="EXTERNAL NADH-UBIQUINONE OXIDOREDUCTASE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22366">
        <property type="entry name" value="NDH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="9">
        <location>
            <begin position="25"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="External alternative NADH-ubiquinone oxidoreductase-like C-terminal" evidence="10">
        <location>
            <begin position="376"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH33937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="466" mass="52028" checksum="4FE2CE8199D567A4" modified="2018-07-18" version="1">MFDSESKYYAHYIGLHKSISSSTKKILILGGGFGGITALKEIEKNFQKESVEITIVSDKNSFLFTPMLPQVASGLLHASNITIPIRYFCKKAKYLHASIDSVDLDNQLVTIERSFDHKVRTLEYDYLILALGAQTNFFHNEDLKKHSFTIKSIEDAIAIKNHIIVMLEHAAQTGNYELQRTLLTFVVVGAGFAGVETISEINQFVKKSVSKSYPSINPKNINMILISSKDRILPEINEKLSEKATIFLEKSGVTIMKNTKADGADEENVMLSNGGKLSCATLIWTGGTKMDKVISDLNCEHDANKRIIVDQSLRMKNKKNVFVLGDCALIKDESADTFYPSTAQHAIREGKLVAENLMLVFNNRKNLKKFTFHSLGIMAIIGNKVGIATIGGRNISGFSAWLLWRMYYLSKIPSFGKKLKISVDWFTDSILAPDVTHIGLIKKKEVNSIHVNENIPSIKEQLLSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQY8</accession>
    <name>A0A2S2KQY8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thioredoxin domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876672.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQY8"/>
    <dbReference type="GeneID" id="76208848"/>
    <dbReference type="OrthoDB" id="28016at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02955">
        <property type="entry name" value="SSP411"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024705">
        <property type="entry name" value="Ssp411"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004879">
        <property type="entry name" value="Ssp411-like_TRX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42899">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42899:SF1">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03190">
        <property type="entry name" value="Thioredox_DsbH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006402">
        <property type="entry name" value="UCP006402_thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spermatogenesis-associated protein 20-like TRX" evidence="1">
        <location>
            <begin position="4"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="675" mass="76735" checksum="58E70F1B4FEBDD99" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR37</accession>
    <name>A0A2S2KR37_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_07060</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Flagellin is the subunit protein which polymerizes to form the filaments of archaeal flagella.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Archaeal flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the archaeal flagellin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000003">
        <property type="protein sequence ID" value="GBH33915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876573.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR37"/>
    <dbReference type="GeneID" id="76208970"/>
    <dbReference type="OrthoDB" id="3285at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097589">
        <property type="term" value="C:archaeal-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013373">
        <property type="entry name" value="Flagellin/pilin_N_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002774">
        <property type="entry name" value="Flagellin_arc-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02537">
        <property type="entry name" value="arch_flag_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35903">
        <property type="entry name" value="FLAGELLIN B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35903:SF1">
        <property type="entry name" value="FLAGELLIN B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01917">
        <property type="entry name" value="Flagellin_arch-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0974">Archaeal flagellum</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="237" mass="24877" checksum="182FA495A0E6EDE9" modified="2018-07-18" version="1">MKLQRKGTRHSHRGVIGVESAIVMIAFVIVAAALAFVVLNMGFTTSQKAKTSIISSLGESSSSMQVAGKVIGQGYVNGGSLNATAFPFKVSSGGSDVNLDAETMAVKYVSNGENYDNIYVGTLSTQEWKQLSAASIQADDSETSGTGEAFADIVGDPFSNETPTNTRAFVYFTQANESPPNDILSQGETAVLAILYNAADKPSALDKVKIEIITPTGAALTVERQVPTITNTIVDLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR40</accession>
    <name>A0A2S2KR40_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876666.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR40"/>
    <dbReference type="GeneID" id="76208855"/>
    <dbReference type="OrthoDB" id="10547at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="229" mass="26682" checksum="BA404A4B20763DE2" modified="2018-07-18" version="1">MKKKKIQNIIIAVIAVIIIGAIGAYNFSVEQTKQKGLQFGIELEKIQEEVKELQIKFYSEKIKWEEGDISKEELLKFYEKHLRDFDEIISKYDKLSPPEIFKSSVELLKLSSESQLESDSQFIEWIKTGDESAKIRSDAQIQESLEYEMLGLVEFYSAKTGVKNYDEPEKFEAPKTGLTQKVIQVSENMKKNCDDEYKNELGEFDSDDIEIEWFNCINEAKKWKVDHLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR47</accession>
    <name>A0A2S2KR47_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">5,10-methenyltetrahydrofolate synthetase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_09270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876761.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR47"/>
    <dbReference type="GeneID" id="76208742"/>
    <dbReference type="OrthoDB" id="10385at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38755">
        <property type="entry name" value="5,10-METHYLENETETRAHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38755:SF1">
        <property type="entry name" value="METHYLENE-TETRAHYDROFOLATE REDUCTASE C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="FAD-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="249" mass="28182" checksum="2A86742608475C60" modified="2018-07-18" version="1">MTIRYEANPPKILPDVDTNESISKFIEKIKIISKKCDAIHLTENVLGYQRVSPIEVGKIIRKQIPDLPITVSLRVRDKSEDEILEFVESCIDVGFSGILVLMGDPSQSGKQDSGQIPSRIVKKLREMKLDSKIDLYLSISNKPDFSKIGKKLESKPKGFMTQVVQNIEQVKNLADNLKEFLVIPIILFPSEKNEKSAKFLNLDLTSYSHGFEEFVKRVHDVTGDILITSPSDFNGLNEFLEKFSNHSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR65</accession>
    <name>A0A2S2KR65_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ArsR family transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_09530</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876786.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR65"/>
    <dbReference type="GeneID" id="76208717"/>
    <dbReference type="OrthoDB" id="10985at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="113" mass="13055" checksum="E10735A502BC84DC" modified="2018-07-18" version="1">MQELIQSQKIVDDERKQVILEILADKYCKQILHNTLEKPKSAMEISHEKKIPISTVYRRLQTLYDAKLLAISGSINQDGKKYFLYKSKVKSISLKCDLEVTTVEFVPNKSHSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR68</accession>
    <name>A0A2S2KR68_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08220</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866845.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR68"/>
    <dbReference type="GeneID" id="76208851"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="151" mass="17558" checksum="EE66BDE9C9A6BC46" modified="2018-07-18" version="1">MWKLETDDGFFRIYDSKKMVAGYFDPDYGDIFPKENSEEIVASMLKNHDKILGGVVMVPLVKFGLFDTDLDTDINTIEQNITRVNEHLQKWKNFLSDTDRQTHSIRISHTDQDMLTITFEVKFSKPTPLETKHLQEELFTTLDLLQKSGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR80</accession>
    <name>A0A2S2KR80_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Short-chain dehydrogenase/reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_09810</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876810.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR80"/>
    <dbReference type="GeneID" id="76208688"/>
    <dbReference type="OrthoDB" id="10157at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05374">
        <property type="entry name" value="17beta-HSD-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051911">
        <property type="entry name" value="SDR_oxidoreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43976">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43976:SF16">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="276" mass="30540" checksum="A1E8F6E1A5C00453" modified="2018-07-18" version="1">MEKVALVTGSSSGIGLETALALSRDGYKTFASMRNTKKAGEIEQAAKKENLSVEIIELDVDREESIVSTIKKIIADCGRIDVLVNNAGYGQFGCTEDVSIDDFRKQFETNFFSIIRIIQEVAPIMRKQKSGNIINISSVAGRMGLPGSPAYISSKFALEGLGECLRYELGQFGIKTTLIEPGVIKTNFFESMKIPDSKTDPKYKELTDHILAGLKMMVQMGTSPSEVAKVIMKAIHDDEMLPRYIVGTDAAMFMEAKKMKTDMEFEKYLSKELFPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR84</accession>
    <name>A0A2S2KR84_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA primase DnaG</fullName>
            <ecNumber evidence="9">2.7.7.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dnaG</name>
        <name evidence="11" type="ORF">NZNM25_09500</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Also part of the exosome, which is a complex involved in RNA degradation. Acts as a poly(A)-binding protein that enhances the interaction between heteropolymeric, adenine-rich transcripts and the exosome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ssDNA + n NTP = ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate.</text>
            <dbReference type="EC" id="2.7.7.101"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Forms a ternary complex with MCM helicase and DNA. Component of the archaeal exosome complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the archaeal DnaG primase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.101" evidence="9"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876784.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR84"/>
    <dbReference type="GeneID" id="76208720"/>
    <dbReference type="OrthoDB" id="8643at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01029">
        <property type="entry name" value="TOPRIM_primases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1360.10:FF:000028">
        <property type="entry name" value="DNA primase DnaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00007">
        <property type="entry name" value="DNA_primase_DnaG_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050219">
        <property type="entry name" value="DnaG_primase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020607">
        <property type="entry name" value="Primase_DnaG_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034154">
        <property type="entry name" value="TOPRIM_DnaG/twinkle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003108">
        <property type="entry name" value="PRK04031.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30313">
        <property type="entry name" value="DNA PRIMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30313:SF2">
        <property type="entry name" value="DNA PRIMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13662">
        <property type="entry name" value="Toprim_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56731">
        <property type="entry name" value="DNA primase core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="9" id="KW-0271">Exosome</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2 9" id="KW-0639">Primosome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Toprim" evidence="10">
        <location>
            <begin position="171"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="381" mass="41072" checksum="FE0852C4D554FCC5" modified="2018-07-18" version="1">MSGKSGIVKYHVKLSYEVDGLVERADIIGAIFGQTEGLLGPEMNLNELQRVSKVGRIEVNTKSTSNTTSGDALIPMSTDIDTCALIAAGIESIDKVGPFDCKFRLEAIDDVRAAKKDDIVRRAKEIKQKWATKTVSEGETMLNDVHQGDAGKLATYGPSKLTCSSGVFDSNWVILVEGRADVINLLRAGYDNVLAIEGAKIDESIKELCNSKETVVAFLDGDRSGGFILKELKSVVTLDYELQADTGVEVEELTPQRIDEILRPIADEIKSGKPAPALKSEDDKPLAEIASKVFPNLNETLEAVALDSDQNEVFKVPISEVVSKLSSQSGIKYLLLDGIITQRLLEGAKNAGVECVVGHRVAKLSNSDGMTLKTFGDLGVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRA5</accession>
    <name>A0A2S2KRA5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GMP synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_09700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876800.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRA5"/>
    <dbReference type="GeneID" id="76208699"/>
    <dbReference type="OrthoDB" id="7388at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01741">
        <property type="entry name" value="GATase1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000033">
        <property type="entry name" value="Glutamine amidotransferase class-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044992">
        <property type="entry name" value="ChyE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695:SF5">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="1">
        <location>
            <begin position="26"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="227" mass="25529" checksum="B35F1E283AC1D779" modified="2018-07-18" version="1">MSDVLLVQNTRIEGSGHLGELLKEDGFEITSVNAKHEPLPEKKFSLAVILGAPESANDDLAYLKAEQKLIKDYVQENIPVLGICLGSQLIAKTFGAKVYPGPKKEIGFYNDLKISDNASFFSSFTNPFTVFHWHGDTFDLPEGAIRLASSKNYLNQAFQYKSAIGLQFHLEVNEEMVNLWLDNAEEKLQKIPYIDPQKIRSDIVDNISTVKSNMNNFYNNFKSSFAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRA6</accession>
    <name>A0A2S2KRA6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Lipoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_09350</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the bacterial solute-binding protein SsuA/TauA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876769.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRA6"/>
    <dbReference type="GeneID" id="76208735"/>
    <dbReference type="OrthoDB" id="10037at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13553">
        <property type="entry name" value="PBP2_NrtA_CpmA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010067">
        <property type="entry name" value="ABC_SsuA_sub-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044527">
        <property type="entry name" value="NrtA/CpmA_ABC-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01728">
        <property type="entry name" value="SsuA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30024">
        <property type="entry name" value="ALIPHATIC SULFONATES-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30024:SF47">
        <property type="entry name" value="TAURINE-BINDING PERIPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13379">
        <property type="entry name" value="NMT1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="341" mass="36917" checksum="937A017C5DACA9DD" modified="2018-07-18" version="1">MKTRSIISAGIAGIILLTIMGIALNSSVATHENKIRIAYFPNIGHSIPIVGMEKGFFQKSVGDEIKIEPRIFDSGPQAIESLFANSVDLAYVGPGPAINGFLNSENHNVKILAGAASGGSSFIVHPLSEIKSASDFAGKKIAAPQIGNTQDVSLRNYLSENGLKPAEKGGSVIVYNIPNPDIYTLFVKGDIDGAWVAEPWATILETELGGKRLFHEEELWPNNEFASVLLIANVDYVEKNHKSVSDLLASHHETADWINQNPTETRIVFNNFLKSHLGKSLSDEVVDVALSNVVITSDPLLDSVYSFAKKSDALGYLGRNGYDLSGIFYPVDSNSILEESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRB1</accession>
    <name>A0A2S2KRB1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">M48 family peptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08720</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876712.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRB1"/>
    <dbReference type="GeneID" id="76208798"/>
    <dbReference type="OrthoDB" id="7216at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="14686" checksum="497D2B3C5C8DEDF8" modified="2018-07-18" version="1">MKLDPELRLQILEKVGIYSNRFSIPEPRILLSTKEVLDAPKEMTEGRRTSAYKYYGVSYLQHNLVFINVRKIPDEKTLENTLVHELIHMRFPYLAHGKRFNKLVRQGLGGKTFLPYRKRNKISAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRF9</accession>
    <name>A0A2S2KRF9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_10240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866857.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRF9"/>
    <dbReference type="GeneID" id="76208641"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="277" mass="31909" checksum="C6E7899524FA497A" modified="2018-07-18" version="1">MTKLLYASIIASVIVMSVFSADAFAQSDFKKIEGDDLNNPVSQDILAKIEFAKKQFMQAKETENKRNAQQKLIDEQRAISQESLKQELQRMEKTYEEFTPRNAFAKYVSNLNATNHGIFWDQFDYLHAKISLARDARDSVIKQGGTFSEAMKQYVQFAKMPKIEMQNIVRELNIKHNLAQEDIQSNFDMNGKLPRYENDIEAPCYGCTAKISKVQIDSKQSVPITRTVFEPKPTQISDLKDSLSDLQSQFLESKNIIAQKKMVFEMNEIIKRIQELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRK4</accession>
    <name>A0A2S2KRK4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2024 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876710.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRK4"/>
    <dbReference type="GeneID" id="76208801"/>
    <dbReference type="OrthoDB" id="5685at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.510.10">
        <property type="entry name" value="NE1680-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018592">
        <property type="entry name" value="DUF2024"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023122">
        <property type="entry name" value="NE1680-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09630">
        <property type="entry name" value="DUF2024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160766">
        <property type="entry name" value="NE1680-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="85" mass="9610" checksum="3971C04E4D537653" modified="2018-07-18" version="1">MEIHVYDTYVKAADGHTMHFDVITGEKDHDKAITYGKEWLESIGEGQAEMTTNECQFCHSQGAPEPVEQAIKEKGYFIQKMEGCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRM5</accession>
    <name>A0A2S2KRM5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA topoisomerase 1</fullName>
            <ecNumber evidence="3">5.6.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">DNA topoisomerase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">NZNM25_09820</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type IB topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876811.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRM5"/>
    <dbReference type="GeneID" id="76208687"/>
    <dbReference type="OrthoDB" id="9279at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.11.10">
        <property type="entry name" value="DNA Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.15.10">
        <property type="entry name" value="Topoisomerase I, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.41">
        <property type="entry name" value="Yeast DNA topoisomerase - domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013034">
        <property type="entry name" value="DNA_topo_DNA_db_N_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013030">
        <property type="entry name" value="DNA_topo_DNA_db_N_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001631">
        <property type="entry name" value="TopoI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025834">
        <property type="entry name" value="TopoI_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014711">
        <property type="entry name" value="TopoI_cat_a-hlx-sub_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014727">
        <property type="entry name" value="TopoI_cat_a/b-sub_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013500">
        <property type="entry name" value="TopoI_cat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008336">
        <property type="entry name" value="TopoI_DNA-bd_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036202">
        <property type="entry name" value="TopoI_DNA-bd_euk_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013499">
        <property type="entry name" value="TopoI_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051062">
        <property type="entry name" value="Topoisomerase_IB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290:SF3">
        <property type="entry name" value="DNA TOPOISOMERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290">
        <property type="entry name" value="DNA TOPOISOMERASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14370">
        <property type="entry name" value="Topo_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01028">
        <property type="entry name" value="Topoisom_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02919">
        <property type="entry name" value="Topoisom_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00416">
        <property type="entry name" value="EUTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00435">
        <property type="entry name" value="TOPEUc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56741">
        <property type="entry name" value="Eukaryotic DNA topoisomerase I, N-terminal DNA-binding fragment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52038">
        <property type="entry name" value="TOPO_IB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="DNA topoisomerase I eukaryotic-type" evidence="10">
        <location>
            <begin position="146"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="460"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="553" mass="64635" checksum="A718CA14D16B26CC" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRP1</accession>
    <name>A0A2S2KRP1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PEFG-CTERM sorting domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_09160</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876751.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRP1"/>
    <dbReference type="GeneID" id="76208754"/>
    <dbReference type="OrthoDB" id="3313at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="288" mass="30451" checksum="5CB173590F4326B8" modified="2018-07-18" version="1">MNNVNKLQSDRLKPFPNVSSLLKISLGLVLFTIISTALVYAETISVDVGGTSYNVDYTATGMTLDAIEADLDFVSLILAVSVPDSDATLDITFERSFFDSTFDGLDDDFIILVDGDEPTFTETETTTQSRTLSIDLPAGTEEIEIIGSSFGESASVPEETPVEEPVEETPVEEPVEETPVEEPVVEETPVVTTPKVEEKPVVTTPKVQCGPGTILKDGTCVLDERCGPGTVLKDGACVLDSPPVLVPAETSVKGMGKETIVAFVAAFVIAGTVGVILALISKASKSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRR0</accession>
    <name>A0A2S2KRR0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper-containing nitrite reductase</fullName>
            <ecNumber evidence="3">1.7.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">NZNM25_10480</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>nitric oxide + Fe(III)-[cytochrome c] + H2O = Fe(II)-[cytochrome c] + nitrite + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16301"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.7.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>Cu(+)</name>
            <dbReference type="ChEBI" id="CHEBI:49552"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotrimer.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.7.2.1" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRR0"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050421">
        <property type="term" value="F:nitrite reductase (NO-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd11024">
        <property type="entry name" value="CuRO_1_2DMCO_NIR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001287">
        <property type="entry name" value="NO2-reductase_Cu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF394">
        <property type="entry name" value="FI03373P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00695">
        <property type="entry name" value="CUNO2RDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 10" id="KW-0186">Copper</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="12">
        <location>
            <begin position="93"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="11">
        <location>
            <begin position="213"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601287-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH34257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="327" mass="37143" checksum="13FB0E87920C4860" modified="2018-07-18" version="1">MVAISAIFFVFPEGINAQKEEKTFVTHTGGVVKTSGEVLDPVYQISEVEFDPDKYLREFNYGRVSLENGQTVREFTIIADDDKVQEISPGVFYNVWTFNGTVPGPTIRATEGDLLRINFINNGDKEHTMHFHGEHPAEMDGVFEIVGGNGGQFTYEFEAGPAGVHPYHCHVMPLEEHIAHGLYGVFIVDPKEGRPPADEMVMVLNGFDTDFDTENNFYAANSIPFYYQHHPIQINKDELVRVYVVNMVEFDPINNLHLHGNLYQYYPTGTDVIPSTFTDMITLSQTERGIMEFKYQYTGKYLFHAHKVEFSEKGWVGVFLVKDDGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRR5</accession>
    <name>A0A2S2KRR5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Putative pterin-4-alpha-carbinolamine dehydratase</fullName>
            <shortName evidence="4">PHS</shortName>
            <ecNumber evidence="4">4.2.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">4-alpha-hydroxy-tetrahydropterin dehydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Pterin carbinolamine dehydratase</fullName>
            <shortName evidence="4">PCD</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_11300</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>(4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin = (6R)-L-erythro-6,7-dihydrobiopterin + H2O</text>
            <dbReference type="Rhea" id="RHEA:11920"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:43120"/>
            <dbReference type="EC" id="4.2.1.96"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the pterin-4-alpha-carbinolamine dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.96" evidence="4"/>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876973.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRR5"/>
    <dbReference type="GeneID" id="76208527"/>
    <dbReference type="OrthoDB" id="10495at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008124">
        <property type="term" value="F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00914">
        <property type="entry name" value="PCD_DCoH_subfamily_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.20">
        <property type="entry name" value="Transcriptional coactivator/pterin dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00434">
        <property type="entry name" value="Pterin_4_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036428">
        <property type="entry name" value="PCD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001533">
        <property type="entry name" value="Pterin_deHydtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002017">
        <property type="entry name" value="PRK00823.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002018">
        <property type="entry name" value="PRK00823.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599:SF0">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01329">
        <property type="entry name" value="Pterin_4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55248">
        <property type="entry name" value="PCD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="91" mass="10672" checksum="19808002C807EF31" modified="2018-07-18" version="1">MRLSQLEIDEQLKNLPGWIIVNEKLHKEFKFENFNQAFGFMTRAAMEIEKMNHHPEWFNVYNRITIELTTHDAGGITKNDINLAKILNSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRR8</accession>
    <name>A0A2S2KRR8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_160049215.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRR8"/>
    <dbReference type="OrthoDB" id="12347at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="353" mass="40088" checksum="53F2F81F4B5DF7B9" modified="2018-07-18" version="1">MNEKFVLFGVLIAILMGGSFGIFSIEDSFAEDNLSVSREDSEAYKKQIEKQEEQKQKAEERKQKLEEKQEEQKQKAEERKQKLEEKKIKLDSNRKKLEEKLSEKINKYEEKLREIKEKYLNRFNSDFSGDTEKFSTAALNLDNEINKKSEEIRKKLEEKQEKLDFRTQRILEKINDGDYLGDKISSSTKNETYELVFDSVTATGIHNNTQSSFLEGNMIFKIFDKSKSNLKLELQECHILVDNVPYNCGFGKARTITSGDSGVKDSLVILAFLEDAVIEEVHSTLKISLSADSPIGDIDDSSQVSILGPQSKISGMWFLDGTGTLTRTVLTIDDSTDDEITVELNESIGVSGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRU6</accession>
    <name>A0A2S2KRU6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRU6"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="121" mass="14635" checksum="EC44DD8EAD242E1E" modified="2018-07-18" version="1">MYRFETKFYLLENSIEIVLSFRIIKLDIFQSYFFGEVEFRSDIFKVNIQNQRRGKVLKLPFELKSKNDKVIVRMTGADDLFVEDYLPYKGESEWLEIDSDEITYFLADHQDQFDTIEIMFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRU7</accession>
    <name>A0A2S2KRU7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Universal stress protein YxiE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">yxiE</name>
        <name evidence="3" type="ORF">NZNM25_11250</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877025.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRU7"/>
    <dbReference type="GeneID" id="76208532"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF25">
        <property type="entry name" value="USPA DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="5"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="141" mass="15524" checksum="A453F27333761DA6" modified="2018-07-18" version="1">MGTQIKKILVPLDGSSNSFRGLDFAIHMARECHATITGLYVAGIAKPRTNDPITPLEKILLDNAQKIMKKAKLKSAQKGILFFDRVTYGDDGKRIVETAEKHNFDLIVIGSRGMGAAKEIFLGSTSNYVLHKSKKPVLIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRU9</accession>
    <name>A0A2S2KRU9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Radical SAM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_10620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRU9"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42731:SF4">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42731">
        <property type="entry name" value="SLL1084 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Elp3/MiaA/NifB-like radical SAM core" evidence="1">
        <location>
            <begin position="271"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="577" mass="65402" checksum="A59E45285CFED238" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRV0</accession>
    <name>A0A2S2KRV0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">MFS transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_09760</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876805.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRV0"/>
    <dbReference type="GeneID" id="76208693"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050171">
        <property type="entry name" value="MFS_Transporters"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23517:SF13">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23517">
        <property type="entry name" value="RESISTANCE PROTEIN MDTM, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="267"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="290"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="325"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="355"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="4"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="384" mass="41646" checksum="D5A1F68B41BD38D9" modified="2018-07-18" version="1">MNRVLILVNITGLIIGISYGLHGPILPVFAKNVIGATYSELGLIGLTNFIPYMFIPLFVGILLDRINNGYLLALGAAVNSTSIYLLSIAQSVPEIMGFRIMTGIAHAFFWPPCESIISNESTEKNRVRNISWFTMFFVIGFMVGPLLGTAVLESLDVTYRILFQIAAFILATGIITSLLASRKSVRNHHEGFSFSSIKEMKKFPEAIILLIFCTSSFGIILTIYPAFLNDRGMTDSDILSLYFVFGISRVVSLALAGKLARNTSRTLIAGTSAASIGLAMSIFADSTVTFGIALVLMGFGFSIFFPLTLEIVLSKTRKAISGKIIGAYETIFGMGWAIGPTIGGPIAQSFGNETPYALFCIIGIGVVIFAIISRKKLEPQRVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRV7</accession>
    <name>A0A2S2KRV7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">N5-carboxyaminoimidazole ribonucleotide mutase</fullName>
            <shortName evidence="3 4">N5-CAIR mutase</shortName>
            <ecNumber evidence="3 4">5.4.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">5-(carboxyamino)imidazole ribonucleotide mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">purE</name>
        <name evidence="7" type="ORF">NZNM25_12010</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + H(+) = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate</text>
            <dbReference type="Rhea" id="RHEA:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="EC" id="5.4.99.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AIR carboxylase family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.18" evidence="3 4"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRV7"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00943"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034023">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01929">
        <property type="entry name" value="PurE_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033747">
        <property type="entry name" value="PurE_ClassI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024694">
        <property type="entry name" value="PurE_prokaryotes"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01162">
        <property type="entry name" value="purE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23046:SF2">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23046">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001338">
        <property type="entry name" value="AIR_carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52255">
        <property type="entry name" value="N5-CAIR mutase (phosphoribosylaminoimidazole carboxylase, PurE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1 3 4" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurE" evidence="6">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="3"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR001338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001338-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="179" mass="19398" checksum="3207A2A2C74EB287" modified="2018-07-18" version="1">MGSSSDSRVMQDASKVLDQFKIKHEDQIVSAHRTPSRLAEYAQHAEKMGFHVIIAGAGGAAHLPGMIASHTVIPVIGVPILVYNDKHPQKNNTSKFSAFGGLDSLLSITEMPSGSPVVAVGINKAGNAAIYAMKILANEFPELKKKLIQHKSNQHDSVIKESEQLKKEGLSKFAKNKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRX1</accession>
    <name>A0A2S2KRX1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_09960</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_264953832.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRX1"/>
    <dbReference type="GeneID" id="76208671"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF8">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13431">
        <property type="entry name" value="TPR_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="23"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="57"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="91"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="125"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="227"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="339" mass="37874" checksum="5B6D663F00F721F3" modified="2018-07-18" version="1">MEQKYEEAINEYDKILKIDPEHIDALNHKGEIRLLQGKYVKALQNFEKVINLDSSNTKAQNGLGYALYNLDRYDAALDQFHTVLEVDNRNNDALIGSGRVLLKLEQYDGAILFFDLVLEKKPDELTALILKAYALANTNQHNSALSIYDKVLEIDPDNVDALIGKGNYYLDLQEFKTSKILYEGALETNAENIDALLGLAEINLLEGKPKKSGQIYDKILSLDSDNVKGLIGKGLSVIELDQSAEALKYFDEALEIDPDNLSALDGKNILLQSKVDTSLDYILIITVVGSITSIVSLILVITKIKENAELQTKIAISDEQIEDLVLKMMKYSEKSKTKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRX7</accession>
    <name>A0A2S2KRX7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ArsR family transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_12130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877061.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRX7"/>
    <dbReference type="GeneID" id="76209291"/>
    <dbReference type="OrthoDB" id="2756at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="252" mass="27899" checksum="68ABAFE8819099A7" modified="2018-07-18" version="1">MSNNNLHADNSVNIFSLDDEKLKILAKVISNKSSIEILNLLFYNELTANEIAQKTNMSLQLVKYYLDKMQQIELIQISKTEKNCKARNMNYYKTSNLAIIITPSKITEKTKQSKLLIRSFNSISKFFGMGIVSAITALSLVMVTAESSLLNPIKNWYSDFILPVKISGTGLVNSVDESLYMAKTKVDSVVSNPGAGSGTPYFDPYSSLLNFTGSDFTIVMLVFAGIGAALSFVVFNRVIGNFQKNILDSPQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS25</accession>
    <name>A0A2S2KS25_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcriptional regulator MntR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mntR_1</name>
        <name evidence="7" type="ORF">NZNM25_10490</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DtxR/MntR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS25"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000189">
        <property type="entry name" value="HTH-type transcriptional regulator MntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.60.10:FF:000005">
        <property type="entry name" value="Transcriptional regulator MntR protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.10">
        <property type="entry name" value="Iron dependent repressor, metal binding and dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050536">
        <property type="entry name" value="DtxR_MntR_Metal-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001367">
        <property type="entry name" value="Fe_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036421">
        <property type="entry name" value="Fe_dep_repressor_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022687">
        <property type="entry name" value="HTH_DTXR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022689">
        <property type="entry name" value="Iron_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238">
        <property type="entry name" value="IRON (METAL) DEPENDENT REPRESSOR, DTXR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238:SF7">
        <property type="entry name" value="IRON-DEPENDENT TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02742">
        <property type="entry name" value="Fe_dep_repr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01325">
        <property type="entry name" value="Fe_dep_repress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00529">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47979">
        <property type="entry name" value="Iron-dependent repressor protein, dimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50944">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH dtxR-type" evidence="6">
        <location>
            <begin position="29"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="162" mass="18796" checksum="586620DA4BCF255B" modified="2018-07-18" version="1">MIDNDSNLSDKKEKRLNSIKKAHSQKNSFTTRMEDYLEVISELVELKGYASPTDISNYMDVRPPSVTKMLRRLDADGYLEYTKYRGLKLTLKGMNIANDIRQKHSDLLDFFEIIGVDGSIANKDVEGIEHHINPKTMKQLRKFTTFLKSNPDLLYSFNRARF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS31</accession>
    <name>A0A2S2KS31_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Gamma-glutamyltranspeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_12810</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877113.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS31"/>
    <dbReference type="GeneID" id="76209219"/>
    <dbReference type="OrthoDB" id="183046at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051792">
        <property type="entry name" value="GGT_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000101">
        <property type="entry name" value="GGT_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00066">
        <property type="entry name" value="g_glut_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43199">
        <property type="entry name" value="GLUTATHIONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43199:SF1">
        <property type="entry name" value="GLUTATHIONE HYDROLASE PROENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0865">Zymogen</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="543" mass="60773" checksum="51098BF79B6B3676" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS49</accession>
    <name>A0A2S2KS49_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_014963880.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS49"/>
    <dbReference type="GeneID" id="40722114"/>
    <dbReference type="OrthoDB" id="5956at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="89" mass="10496" checksum="447A5D65E1266E29" modified="2018-07-18" version="1">MTPIVGTFLSILSSLTGQLGPNYDPLFYDVMIYIFGTMMFTIFLLGIISFWLRVHGWAYKDNRERWVDELDRHFEREGIGLIPDNGKYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS51</accession>
    <name>A0A2S2KS51_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription regulator TrmB N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_10760</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS51"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="13490" checksum="3E1FBC403CD48A0D" modified="2018-07-18" version="1">MMDLSRIENDLISEIKLDPIQAKTYLLVTCFGKMSPAKIAEKLNISVEDAKKASRDLITLGAFIDITDTEFEAMHPRFTAVNMYRRMCERENIPFGRNKIVDTIGVILEKSYDDARTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS89</accession>
    <name>A0A2S2KS89_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Blue (type 1) copper domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_11090</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876958.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS89"/>
    <dbReference type="GeneID" id="76208549"/>
    <dbReference type="OrthoDB" id="11836at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000923">
        <property type="entry name" value="BlueCu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052721">
        <property type="entry name" value="ET_Amicyanin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507:SF1">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00127">
        <property type="entry name" value="Copper-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Blue (type 1) copper" evidence="3">
        <location>
            <begin position="56"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="136" mass="15242" checksum="0C7B832B4FF33774" modified="2018-07-18" version="1">MIKKITILSVIGVALIAILASSLSNNSDDENQETSDVILKGDIIMPTKVSRPGCEVKDICYIPSKIFIEKGKQVTWINEDSAFHSVTSGFYNEPTELFDSGYLDPFESFSLTFDNEGTYDYFCTLHPWMKGQVIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSA3</accession>
    <name>A0A2S2KSA3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphate/phosphite/phosphonate ABC transporter substrate-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_12380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866881.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSA3"/>
    <dbReference type="GeneID" id="76209266"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01071">
        <property type="entry name" value="PBP2_PhnD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005770">
        <property type="entry name" value="PhnD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01098">
        <property type="entry name" value="3A0109s03R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35841">
        <property type="entry name" value="PHOSPHONATES-BINDING PERIPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35841:SF1">
        <property type="entry name" value="PHOSPHONATES-BINDING PERIPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12974">
        <property type="entry name" value="Phosphonate-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="363" mass="39417" checksum="77F18502875C442B" modified="2018-07-18" version="1">MKKQKMLTLIAVIAVIGVTTMSLAQNVQAESLVPEWIKSNAGWWADGTVDDETFLNGIRFLIENDIINVSSESNTVDVDTLTIGFIPVEKADELTPKAQSLEKFLESKLGIDVEIVVPTNYETIIEGMRFGHIDGAFMDTGPAWITHQRTGAEAVLAELVAGKVNYQATVWALADNDSIHSIEDTVGKRVAFTSITGSSGFVRPMGTLVTDGHVVIEDGGDIVALESALAKNFESYTFAGGYKAALELLLNDNVDVAFGSDIAPQQYLELEDQVKLRPVTTIGPVPSHVFMVSADMSDSTKNALVDALIELNYDERNYILKDLYGAEALVPTTTTMHIGEFGTFIDALTGLDQVILDSYNKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSA6</accession>
    <name>A0A2S2KSA6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">pyridoxal 5'-phosphate synthase (glutamine hydrolyzing)</fullName>
            <ecNumber evidence="3">4.3.3.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_11360</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + phosphate + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the PdxS/SNZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876980.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSA6"/>
    <dbReference type="GeneID" id="76208521"/>
    <dbReference type="OrthoDB" id="6840at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000001">
        <property type="entry name" value="Pyridoxine biosynthesis protein PDX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001852">
        <property type="entry name" value="PdxS/SNZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033755">
        <property type="entry name" value="PdxS/SNZ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003215">
        <property type="entry name" value="PRK04180.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01680">
        <property type="entry name" value="SOR_SNZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029271">
        <property type="entry name" value="Pdx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51129">
        <property type="entry name" value="PDXS_SNZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0704">Schiff base</keyword>
    <feature type="domain" description="PdxS/SNZ N-terminal" evidence="9">
        <location>
            <begin position="29"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="322" mass="35059" checksum="3DB1C2C94003D334" modified="2018-07-18" version="1">MLPLSGERFDAKGIISDKINSVNTLRGSSTVKRGFAHMLKNGVVMDVTTVEQAQIAEEAGAVSVMVLDKLPSEVRKAGGVARTASIRIIEEIMNSVTIPVMAKCRIGHVNEALVLQETDVDMIDESEVLTPADEFHHIWKWDFTTPVVNGARSLAEALRRIEEGASMIRTKGEPGTGNVAEAVTHIKKVNDELRTIKAIYDSGDNQDLVRIAREFKVSYDIVQQTAKLGRLPVVNFAAGGIATPADAAYLMSLGCDGIFVGSGIFNADDAKERARAIVLATTFWNESDKVKEAQKMIDERQSMIGLDVKTLELRMQDRGGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSC6</accession>
    <name>A0A2S2KSC6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Triosephosphate isomerase</fullName>
            <shortName evidence="5">TIM</shortName>
            <shortName evidence="5">TPI</shortName>
            <ecNumber evidence="5">5.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Triose-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">tpiA</name>
        <name evidence="6" type="ORF">NZNM25_13530</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="5"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877170.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSC6"/>
    <dbReference type="GeneID" id="76209146"/>
    <dbReference type="OrthoDB" id="9465at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00189"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_A">
        <property type="entry name" value="TIM_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022891">
        <property type="entry name" value="Triosephosphate_isomerase_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003302">
        <property type="entry name" value="PRK04302.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="Triosephosphate isomerase (TIM)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 5" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="3 5" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="4 5 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Electrophile" evidence="5">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="5"/>
            <end position="7"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="197"/>
            <end position="198"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="218" mass="23678" checksum="462DC326924364D0" modified="2018-07-18" version="1">MFVINCKNYEEISGDKIIKFVKTAEKISKKYKIKIAIAPPQHLIGLVAKSTIPIFAQHIDDSKIGSTTGFVIPELLKMSKVKGSLINHSEHRISSKEIEKLISKLKELKMTSVLCVKDIAEVKKYVKLNPDYIAIEPPELIGSGKAVSKEKPGLISKAAEAVKNAKNNTELLCGAGIVSGEDVSKAIELGSKGILVASGIVKSKNWDSIMSEFAKSMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSK8</accession>
    <name>A0A2S2KSK8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase M10 metallopeptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_13950</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSK8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF8">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="34"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="102"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M10 metallopeptidase" evidence="8">
        <location>
            <begin position="360"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="478" mass="54943" checksum="22750CE1F16389F3" modified="2018-07-18" version="1">MKNNTMTTRYFLVIILLSSIILTEDAFAQTGKEAEAMFNQATDLFVKGNYKQAITIYDEVLEIAPNNISTLKMKGIAQSNLEDHTNSLKQFFRVLQHSPDDALALTGMGVGFGNLGEYQESISYINRAYNEKPNSVVIKNYKEFIDKVISKYPYAPTEKPKGLEKEYITSIPEWVKPIAKWWSEENIDDAEFVSALLYLIDNKIIQIPPVETQNVSDEIPEWVKNNAGWWADGEIDDEAFVLGIQYMIKQGIIIVNMQDENKKSQEQIEHEYYLFEKYLRDISNNISKEKRYIEFPNPSKDVIKKFLRDYIKWNFEAEVKKASSNFPDPTYEIIDDTYIILYKVFVNEQPTGLPLDHVNTLQNSLAFWEKQELNTNNEKAKIKFEITNQKQNANVWVTWVVRDIGQGVLGHAHLGKGVVEVTLGDYNCDGSFQLYDVKSVETIMTHELGHSIGLKHTTDQGNIMYPSYSPSYAYCLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSL6</accession>
    <name>A0A2S2KSL6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_14300</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829091.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSL6"/>
    <dbReference type="GeneID" id="76209065"/>
    <dbReference type="OrthoDB" id="7373at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="55" mass="6168" checksum="AE921E3F63B2FCE1" modified="2018-07-18" version="1">MGLFKRKKDDKNKTKCDVCGTDLYDPERLKKHLKKAHGNVPAKKLDPNAGDGGTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSL8</accession>
    <name>A0A2S2KSL8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Secretion system protein E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_13320</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866892.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSL8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01130">
        <property type="entry name" value="VirB11-like_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050921">
        <property type="entry name" value="T4SS_GSP_E_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486">
        <property type="entry name" value="TWITCHING MOTILITY PROTEIN PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486:SF6">
        <property type="entry name" value="TYPE IV PILUS RETRACTATION ATPASE PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="297"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="12"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="553" mass="63417" checksum="E8B55E2B67EBEBD9" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSQ7</accession>
    <name>A0A2S2KSQ7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_14870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSQ7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024173">
        <property type="entry name" value="Pesterase_MJ0037-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39323">
        <property type="entry name" value="BLR1149 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39323:SF1">
        <property type="entry name" value="BLR1149 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000887">
        <property type="entry name" value="Pesterase_MJ0037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="28"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="254" mass="28866" checksum="5E5BBC4B97EDFA5C" modified="2018-07-18" version="1">MYSITLMLQTRIIPSKPALILEGEKKNLIVTDIHLGFENTLKSNEIFIGKHSTINETIQEISEIIDSENPDSVILLGDIKSSIKNISRNEWEEVPLFFEEIGKKCDIVLIPGNHDANIQKLIPNKISMISSTGMIEENVLLTHGHTMPSENFSHIDKIIMGHIHPVFFQEDSIMNGQRVWVSLKTEKQKIFPSKSGELEIIIVPSFNRYFYATHKKYYKKSISPIIERLEILSAKIVSLDGVILGDETIIKQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSS4</accession>
    <name>A0A2S2KSS4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12890</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877117.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSS4"/>
    <dbReference type="GeneID" id="76209211"/>
    <dbReference type="OrthoDB" id="374115at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="175" mass="20630" checksum="C07D1E04B6E8F40C" modified="2018-07-18" version="1">MTEMLTDREKLVSVYGFTKYYSDENIPDDFIHMLVEQCVKKNTNLETVDLKKEIEGLDNFLTFTIESTMQNIEKSVLYKRIISDIPKPFTVGDYLKKNAREALIFVYSVGKGIEDASKESNVPFDFQNYIKKFDDWVFYESFSDKEFEEIPKHTLRFLNTVTDEIKRKPESGERE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KST8</accession>
    <name>A0A2S2KST8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_15170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KST8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008964">
        <property type="entry name" value="Invasin/intimin_cell_adhesion"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49373">
        <property type="entry name" value="Invasin/intimin cell-adhesion fragments"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="731"/>
            <end position="752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="757" mass="83335" checksum="99319E14BF4D9A91" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT12</accession>
    <name>A0A2S2KT12_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF432 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13860</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877199.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT12"/>
    <dbReference type="GeneID" id="76209113"/>
    <dbReference type="OrthoDB" id="116710at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007366">
        <property type="entry name" value="DUF432"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04254">
        <property type="entry name" value="DUF432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019202">
        <property type="entry name" value="UCP019202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="28411" checksum="5BFB779AE929ABEF" modified="2018-07-18" version="1">MSQIQPESVFSDYGIYKVIDNLELSLPDITIKIDKIGDHVFSYVRKDAEDNIVKKIIPTKSDDLIIELSPIRPLNYPAKRTSYVYLDFETPLFLSEGSAVTVFVRCPIEIGVFLVHDEHKDSLDCFTCDPNNSRFCLYGDPESGNLCKYALSEIVESYDDSIPFLNGVLKIDIKNDLDKGLTLSKIVFPITGNSIYYKNSKAIIDSLSAIMKKKLTLEIIDVTSDKIQTDWIMSPTFEQIESIKHMDMGVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT13</accession>
    <name>A0A2S2KT13_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Translation-associated GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ychF_1</name>
        <name evidence="3" type="ORF">NZNM25_13890</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877202.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT13"/>
    <dbReference type="GeneID" id="76209110"/>
    <dbReference type="OrthoDB" id="5875at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01669">
        <property type="entry name" value="TGS_MJ1332_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013646">
        <property type="entry name" value="YGR210-like_G4"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007171">
        <property type="entry name" value="PRK09602.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF1">
        <property type="entry name" value="OBG-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08438">
        <property type="entry name" value="YGR210-like_G4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="2">
        <location>
            <begin position="3"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="404" mass="44856" checksum="BBB2DD15129C8C22" modified="2018-07-18" version="1">MPIKLGLIGKTNTGKTTFFNSATLSSEEISSYPFTTKSPVSGIAHAITLCVHPEFKIQDNPNNSKCLDGWRYIPIELIDLPGLIKDAWKGKGLGNQFLSIAAQSDALLHVVDASGGIDSTGKISEVGTGDPISDFADIEEELIMWYHKILEGNREKISKLIRTGTDISDAITDLYRGIGVNKNHVKETFLATGLEEKNFDNFDMVDSKKFVSHLRKISKPTLIVANKVDVEGADKNFARLRERYNDSIVIPVSGDSEFSLRRAEQKGLIKYSPGSEQFEIIKSEDLNEKQVNALNFIKKGIMGEYMRTGVQFAINVAVFKLLKMNSIYPVADETNLTDKKGRILPDLILLKDGATIGDLAKEIHTDLTKGLLYGKDLRYNLRLPVDYQLRDRDVVSLVSAAKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT37</accession>
    <name>A0A2S2KT37_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Nascent polypeptide-associated complex protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">nac</name>
        <name evidence="7" type="ORF">NZNM25_15970</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Contacts the emerging nascent chain on the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Interacts with the ribosome. Binds ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NAC-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877435.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT37"/>
    <dbReference type="GeneID" id="76208389"/>
    <dbReference type="OrthoDB" id="53273at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22054">
        <property type="entry name" value="NAC_NACA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14359">
        <property type="entry name" value="UBA_AeNAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="entry name" value="Nascent polypeptide-associated complex domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00814">
        <property type="entry name" value="NAC_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044034">
        <property type="entry name" value="NAC-like_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005231">
        <property type="entry name" value="NAC_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00264">
        <property type="entry name" value="archaeal-type nascent polypeptide-associated complex protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19026">
        <property type="entry name" value="UBA_HYPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NAC-A/B" evidence="6">
        <location>
            <begin position="3"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="NCBIfam" id="TIGR00264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="113" mass="12738" checksum="25D8BFC7FD04F1E3" modified="2018-07-18" version="1">MMRGGNREMRRMMDKMGLDMNELSNVQEVIIKTDKKEIIITKPSVTEMKAKDNSIFTVTADSYEERELEVPIFSEEDIQLVSQQAGVDEEKAKNALEEAEGDLARAILLLTSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT55</accession>
    <name>A0A2S2KT55_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_14360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT55"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="69" mass="8069" checksum="5B40EAD3C23CA786" modified="2018-07-18" version="1">MKVLRVNRLRAAILSDAIDQIGKFKTVERLVNLNADRKRLWLDQIQSVESKTKNDSMPILLSHFYKETI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT56</accession>
    <name>A0A2S2KT56_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_15280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT56"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="89" mass="9514" checksum="9396F98A7A888D26" modified="2018-07-18" version="1">MSFTGMKRIPLTQNSFSCLISKCLHAQSALTYATPTFGSIVVKGKLPDGTEVSVAAEKNVDFPTFALPSKPICNIVKNSILLISIAEIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTA6</accession>
    <name>A0A2S2KTA6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_14860</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877284.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTA6"/>
    <dbReference type="GeneID" id="76209009"/>
    <dbReference type="OrthoDB" id="12242at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28498">
        <property type="entry name" value="ZINC FINGER SWIM DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28498:SF1">
        <property type="entry name" value="ZINC FINGER SWIM DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="30"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="100" mass="11633" checksum="215F8EFD658F3AE2" modified="2018-07-18" version="1">MNKDFDRVEVLVSEKRVKLHVFQPSQRKIWTVVGKGEEHWLDPNGSYCSCPGFYFGQLNGKNTCYHLDSVKLAQKENKIEKIIFSDDEFDDFLFGLISDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTE0</accession>
    <name>A0A2S2KTE0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Coenzyme A biosynthesis bifunctional protein CoaBC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">DNA/pantothenate metabolism flavoprotein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Phosphopantothenoylcysteine synthetase/decarboxylase</fullName>
            <shortName evidence="3">PPCS-PPCDC</shortName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenoylcysteine decarboxylase</fullName>
                <shortName evidence="3">PPC decarboxylase</shortName>
                <shortName evidence="3">PPC-DC</shortName>
                <ecNumber evidence="3">4.1.1.36</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaC</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenate--cysteine ligase</fullName>
                <ecNumber evidence="3">6.3.2.5</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaB</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="3">Phosphopantothenoylcysteine synthetase</fullName>
                <shortName evidence="3">PPC synthetase</shortName>
                <shortName evidence="3">PPC-S</shortName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="3" type="primary">coaBC</name>
        <name evidence="6" type="ORF">NZNM25_16900</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes two sequential steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. In the second step the latter compound is decarboxylated to form 4'-phosphopantotheine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(R)-4'-phosphopantothenate + L-cysteine + CTP = N-[(R)-4-phosphopantothenoyl]-L-cysteine + CMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19397"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="6.3.2.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>N-[(R)-4-phosphopantothenoyl]-L-cysteine + H(+) = (R)-4'-phosphopantetheine + CO2</text>
            <dbReference type="Rhea" id="RHEA:16793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="4.1.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="3">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the PPC synthetase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.36" evidence="3"/>
    <dbReference type="EC" id="6.3.2.5" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877512.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTE0"/>
    <dbReference type="GeneID" id="76208296"/>
    <dbReference type="OrthoDB" id="10536at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071513">
        <property type="term" value="C:phosphopantothenoylcysteine decarboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004632">
        <property type="term" value="F:phosphopantothenate--cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015941">
        <property type="term" value="P:pantothenate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10300">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02225">
        <property type="entry name" value="CoaBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035929">
        <property type="entry name" value="CoaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005252">
        <property type="entry name" value="CoaBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007085">
        <property type="entry name" value="DNA/pantothenate-metab_flavo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00521">
        <property type="entry name" value="coaBC_dfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359">
        <property type="entry name" value="HOMO-OLIGOMERIC FLAVIN CONTAINING CYS DECARBOXYLASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359:SF6">
        <property type="entry name" value="PHOSPHOPANTOTHENOYLCYSTEINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04127">
        <property type="entry name" value="DFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102645">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="4">
        <location>
            <begin position="30"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/pantothenate metabolism flavoprotein C-terminal" evidence="5">
        <location>
            <begin position="203"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenoylcysteine decarboxylase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenate--cysteine ligase" evidence="3">
        <location>
            <begin position="208"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="418" mass="46047" checksum="EFA9C08791BE148D" modified="2018-07-18" version="1">MSKLVVKKIKKKDHPSLDIVTSKGVELIGKKIVLCVAGSVAAYKAIELARLLMRHGADVTCVTSNAVTKLIQPDYFKWATGNEVITKLTGELEHIRLADYKQSDLVIVYPATANTLGKLANGIDDTPVSTVLTVGFGSKIPILMCLAMHESMYDNLAVKKNIEFLKNKIQFVNPQMVEGKAKAPEPEDVLEIVLKKFGFSSVLKNKKILITAGPTLEYIDPVRVITNISSGKTGVLLASELISAGAKVTIVYGPGNQKPSKGAKVINVTTSKEMFDITKKELKKKFDVVIMAAAASDYTPESVSKSKIKSDKKCLTIRLKKVPKIIEQVKKLQKDTLLVGFKAESNITKSALIKSAQKKMKDVNADIMIANDIGSKYQKNPDRNQILIIDNNKIKSSGWKQKEKIVKLIRKEIEQKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTG7</accession>
    <name>A0A2S2KTG7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">AsnC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_17350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTG7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="1">
        <location>
            <begin position="28"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="92" mass="10410" checksum="89B53996E7224B11" modified="2018-07-18" version="1">MFDENQPVPTAYVLLNSDLGSDESIIAEVKQILADEDVKYEVQGVYGVYDIVLKLTSDDSEKLRTIITNKVRKISKVQSTLTMMVIEEQENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTH9</accession>
    <name>A0A2S2KTH9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_17430</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877551.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTH9"/>
    <dbReference type="GeneID" id="76208242"/>
    <dbReference type="OrthoDB" id="11824at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008217">
        <property type="entry name" value="Ccc1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31851">
        <property type="entry name" value="FE(2+)/MN(2+) TRANSPORTER PCL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01988">
        <property type="entry name" value="VIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="63"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="227" mass="25445" checksum="DF0E0976B54B690F" modified="2018-07-18" version="1">MKWHFDDFIYGSIDGAVTTFAIVAGVVGASLSPGVIIILGFANLFADGFSMATSNYQASKARNEFVQMKRRQEEWEIDNLEEQERDEIREIYKEKGFKDELLEDVVRIITSRRKVWIDTMMKEELGLIDDEKNPLESSVSTFVGFNLIGLIPLLPFMIFLMIGIESNSDAFIYSTISVLLAFFLVGMFKGKIVRHSMLRSGIITLIIGGLAAGVAYIIGYGLNFLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTJ5</accession>
    <name>A0A2S2KTJ5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">NZNM25_17400</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877548.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTJ5"/>
    <dbReference type="GeneID" id="76208245"/>
    <dbReference type="OrthoDB" id="2830at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004673">
        <property type="term" value="F:protein histidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06225">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033479">
        <property type="entry name" value="dCache_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050398">
        <property type="entry name" value="HssS/ArlS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45528">
        <property type="entry name" value="SENSOR HISTIDINE KINASE CPXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45528:SF1">
        <property type="entry name" value="SENSOR HISTIDINE KINASE CPXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02743">
        <property type="entry name" value="dCache_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158472">
        <property type="entry name" value="HAMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="16">
        <location>
            <begin position="322"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="15">
        <location>
            <begin position="402"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GBH34949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="591" mass="66727" checksum="43EB2B153F21F415" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTJ8</accession>
    <name>A0A2S2KTJ8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_17650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877571.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTJ8"/>
    <dbReference type="GeneID" id="76208220"/>
    <dbReference type="OrthoDB" id="7699at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="94" mass="10676" checksum="2C83BEA12AEC5B4E" modified="2018-07-18" version="1">MSHTGVDVIDFLFYTIYPVIGIFTVEAISRLIKAPKWIKLWVQAAVSMGFGIYYWFVLPAPQNFPLTAMVMFALAIALIYQGRRAKISPDKSPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTL2</accession>
    <name>A0A2S2KTL2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">4-phosphopantoate--beta-alanine ligase</fullName>
            <ecNumber evidence="5">6.3.2.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Phosphopantothenate synthetase</fullName>
            <shortName evidence="5">PPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_16880</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the condensation of (R)-4-phosphopantoate and beta-alanine to 4'-phosphopantothenate in the CoA biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(R)-4-phosphopantoate + beta-alanine + ATP = (R)-4'-phosphopantothenate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27930"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="ChEBI" id="CHEBI:61294"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.2.36"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal phosphopantothenate synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.36" evidence="5"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877511.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTL2"/>
    <dbReference type="GeneID" id="76208298"/>
    <dbReference type="OrthoDB" id="10078at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016881">
        <property type="term" value="F:acid-amino acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12640">
        <property type="entry name" value="Phosphopantoate/pantothenate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02224">
        <property type="entry name" value="PPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002855">
        <property type="entry name" value="PPS/PS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038138">
        <property type="entry name" value="PPS/PS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041123">
        <property type="entry name" value="phpantohe_syn_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010324">
        <property type="entry name" value="PRK13761.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40695">
        <property type="entry name" value="4-PHOSPHOPANTOATE--BETA-ALANINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40695:SF1">
        <property type="entry name" value="4-PHOSPHOPANTOATE--BETA-ALANINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02006">
        <property type="entry name" value="PPS_PS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004853">
        <property type="entry name" value="UCP004853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="182"/>
            <end position="184"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="188"/>
            <end position="189"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="200"/>
            <end position="201"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="254" mass="27869" checksum="7EE2B8753543C051" modified="2018-07-18" version="1">MPLIPKSHPRAKSLLIREKLVKGFDNGLVAKEGLLAQGRGEAFDYLLGEKTTKSAKKAIRAAAAQILLAERPVISINGNIAALCPKEIVKLSKQTKAKLEVNLFYANKNRKQAIIKILKKSGAKVVLGSNISTSTKLPRIDSARRVVDKNGIFVADVVVVPLEDGDRTIALRKAGKTVITFDLNPLSRTSQTANITIVDNVTRAIDLLIYDSKKLSKKNHSSLRKIVDDFDNKKNLSENILEIRKNLTRGAKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTL4</accession>
    <name>A0A2S2KTL4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_15960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877434.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTL4"/>
    <dbReference type="GeneID" id="76208390"/>
    <dbReference type="OrthoDB" id="11763at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="108" mass="11775" checksum="D18ABE3926028D0F" modified="2018-07-18" version="1">MAESKVTGIIKSLNALEDELDSLNSKVGDMKKQLNLKAQSKIDSLLEKTREMATKEAEVIINASKEKATSESQRIAQEGDAKLSEIQSTIDANFDEAVKHVVSTVLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTP7</accession>
    <name>A0A2S2KTP7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription initiation factor IIB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tfb_9</name>
        <name evidence="7" type="ORF">NZNM25_17900</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TFIIB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877594.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTP7"/>
    <dbReference type="GeneID" id="76210331"/>
    <dbReference type="OrthoDB" id="7429at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="125"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="219"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="306" mass="34113" checksum="E2AEF0EE905174C0" modified="2018-07-18" version="1">MLENYSNDYDVKCKLDTCKTYPAITDSERGEIVCGGCGLILVENMSDASYENNGYSQEDFMKLARTGPATSLTMNDKGLSTVIGTNKDSTGKALSNKTKYEFNRLRTWDQRSKSRKTATLSKAFTLLHGMKTKLGIPDNVVENAAYLYRKAVNAKLTRGRTMASLVSASLYAACRENNIPRTLDDVAEAGNVERRILSRDLRTIIKKLELNLNQYDTSSFISKISNNMNLKEKTKRDAFDILKRCEKEDITAGKHPVAQAAASLYLACIMNGEKISQKKFSVESGVSDVTIRNRAVLIKKTLKLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTX1</accession>
    <name>A0A2S2KTX1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_18830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTX1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="117" mass="13414" checksum="6DD3F8923BDB014A" modified="2018-07-18" version="1">MINNHTHEPQTEIIPINSMIGLEKSTIFFHAPQANKLPWGFVEGKVANHVEGYPVIIQIFKNNHAVHFAQTDIGDDGIYEYKFRVLNSDKGHTTKIFEGDYSVKIFKVVYLNQENLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTX2</accession>
    <name>A0A2S2KTX2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Zinc-ribbon domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_18700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877695.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTX2"/>
    <dbReference type="GeneID" id="76210097"/>
    <dbReference type="OrthoDB" id="6984at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="Zn_Ribbon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zinc-ribbon" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="118" mass="12981" checksum="9001FAF9AFDF3947" modified="2018-07-18" version="1">MEVFDGKKAAQDYMSKHTLAFSTPELTLMRFAFWLGDSVPDPKNEGKGIPRMMTYLTEQDFQPVLIDDDKYEPSGAVRSSASVGNAYKEVKTDGKFCSECGTSLSATAKFCPECGTTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTX9</accession>
    <name>A0A2S2KTX9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_18930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877714.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTX9"/>
    <dbReference type="GeneID" id="76210120"/>
    <dbReference type="OrthoDB" id="2830at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00156">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052048">
        <property type="entry name" value="ST_Response_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228:SF1">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ARR22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="1">
        <location>
            <begin position="4"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="125" mass="13928" checksum="2A86779E9244EBD6" modified="2018-07-18" version="1">MNSIILLVEDDTDLISIYKEILELHGYDLQTANNGQEAVEKFKDTNPSLVIMDGDMPILDGYEAFKQIKEIDKNAKVVIVTGYSQLEPKNQQAVKEGLIKVISKPLGVDELLSLAEQYTEAKIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU25</accession>
    <name>A0A2S2KU25_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_19170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877730.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU25"/>
    <dbReference type="GeneID" id="76210146"/>
    <dbReference type="OrthoDB" id="8673at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="77" mass="8956" checksum="A09682D79E18F613" modified="2018-07-18" version="1">MSYEYYRRGRNWFTAIGVLFCVMGGIVLIQQLLIWGPEFVEEFLINSDFTNEKVSAAMIAFGIFMIVLGFRKHGQKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU30</accession>
    <name>A0A2S2KU30_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Radical SAM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_18820</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU30"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051198">
        <property type="entry name" value="BchE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43409">
        <property type="entry name" value="ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43409:SF4">
        <property type="entry name" value="RADICAL SAM SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01095">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="10"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH35091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="292" mass="33342" checksum="EF26AE4B8D65DCC5" modified="2018-07-18" version="1">MLNYDAPLYRPPSEARSLIFQVTLGCSFNECSFCDMYRSKEYSERPWDDVKAEIDMMAKYLPDTRRVFLADGDALNLDSGYMVKIVRYIREKFANIERISCYAMPMNILKKTSEELKKMNEAGLDMFYLGIESGSDIVLKKVTKGAVAKTIIKSVNKAKEAGYVMSCMVILGLGGKKYSKEHIKGTAEVISACSPNYVGALTLYLENGIKQEFLDKYNGEFIRINDDESLDELHDLISQIETKDEIIFRANHGSNAYTIKGTFPQDKQEMMDKVEWMKQHPEIMRPQGLRGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU60</accession>
    <name>A0A2S2KU60_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Stage II sporulation protein M</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_17790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU60"/>
    <dbReference type="OrthoDB" id="10774at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002798">
        <property type="entry name" value="SpoIIM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01944">
        <property type="entry name" value="SpoIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="19607" checksum="56AA2F7DAD76900F" modified="2018-07-18" version="1">MKKIRTVTFFIFLAIFAAAYQLGSMTSVSDEDAKNFMLEFEELVLDIDAFGIFVHNTTIALPMFIPGFGVAWGIFSAWSTGFAFAAITTTAPILADIPPLAILFLSPFGLMELTAYSIGISRSFILIKAITKKVNLTQFIKPTLFEVGIVISLLLAGGYLEFYMLELAQQEGFEMPGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU80</accession>
    <name>A0A2S2KU80_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cation/H+ exchanger transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_17990</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU80"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32507">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32507:SF0">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="155"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="319"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="343"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="405"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="11">
        <location>
            <begin position="54"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="430"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="444"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH35008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="475" mass="51141" checksum="A404D0818C4A08EF" modified="2018-07-18" version="1">MFRGQIDGLETLFNFDSSSILTKITELQSSIAALSIQDGPIASAHVILLAAGVVIFLGVAGEAFFKKTGIPDVAFLMILGVIIGPVFGLIQPEAVIQVVPYFAALALIIIMFDGGLNLDIKHVIRTAHFSVTLAILGFILSVAIITFAAHFALGWLWLESILLGSIVGGSSSAIVFGLVRNIKISEETKSMLSFESALTDILATIIAFILFEAVLVGHFDLQTLEETLGRAVVVGLVLGFGVGIPWMYVSTKLGNAQHAYMLTLGVLFVLFFLANSFGESGALTALVFGLMIGNKRHLSKILRFKLPKIEMDDPTHNQLTFLVRSFFFVFVGLMASFGQVEYLIFGVLITIVIYFGRMFVGKITLTKKFSLLDKAVTNAMIPRGLAAAVLATYPITMGISNAEAYPQLVFFIILSSVIITTIGLGKSKKIPPPESVEGGFVKPTKGDSDEGIFTDDHIDKSVGGGFVKPTEDSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUA9</accession>
    <name>A0A2S2KUA9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Endonuclease III</fullName>
            <ecNumber evidence="13">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">DNA-(apurinic or apyrimidinic site) lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">nth</name>
        <name evidence="15" type="ORF">NZNM25_18290</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Hydrolyzes mismatched double-stranded DNA and polynucleotides, releasing free thymine.</text>
            <dbReference type="EC" id="3.2.2.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="13">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the Nth/MutY family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="13"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877628.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUA9"/>
    <dbReference type="GeneID" id="76210292"/>
    <dbReference type="OrthoDB" id="84708at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141016">
        <property type="term" value="F:G/T mismatch-specific thymine-DNA glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000703">
        <property type="term" value="F:oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1670.10:FF:000001">
        <property type="entry name" value="Endonuclease III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000001">
        <property type="entry name" value="Endonuclease III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00942">
        <property type="entry name" value="Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004036">
        <property type="entry name" value="Endonuclease-III-like_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005759">
        <property type="entry name" value="Nth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43286">
        <property type="entry name" value="ENDONUCLEASE III-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43286:SF1">
        <property type="entry name" value="ENDONUCLEASE III-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001435">
        <property type="entry name" value="Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01155">
        <property type="entry name" value="ENDONUCLEASE_III_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 13" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="15" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11 13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 13" id="KW-0408">Iron</keyword>
    <keyword evidence="7 13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="14">
        <location>
            <begin position="42"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GBH35038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="216" mass="24543" checksum="52DFEB8077E1BBCE" modified="2018-07-18" version="1">MEKILRGMTDTMNSVKPPRITALQDLHDAETGPFSILIGTILSARTKDEATTKAVKALFSKYKNPKELAKAKIKDVEKIIKSIGFYHVKSKRIIEVAKIIDKKYKGKVPDNLDTLVQLPGVGRKTANCVLVYAFEKPAIPVDIHVHRISNRLGLVDTKNPEETEQELMKKIQKKYWIDINDTFVMYGQNICKPISPMCDVCKIKKMCKYYKSKNVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUP7</accession>
    <name>A0A2S2KUP7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutamyl-tRNA(Gln) amidotransferase subunit E</fullName>
            <shortName evidence="6">Glu-ADT subunit E</shortName>
            <ecNumber evidence="6">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">gatE</name>
        <name evidence="8" type="ORF">NZNM25_19960</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of GatD and GatE.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GatB/GatE family. GatE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUP7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.410:FF:000003">
        <property type="entry name" value="Glutamyl-tRNA(Gln) amidotransferase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="entry name" value="GAD-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.380">
        <property type="entry name" value="GatB domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00588">
        <property type="entry name" value="GatE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029351">
        <property type="entry name" value="GAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042114">
        <property type="entry name" value="GatB_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004414">
        <property type="entry name" value="GatE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00134">
        <property type="entry name" value="gatE_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003107">
        <property type="entry name" value="PRK04028.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="GLUTAMYL-TRNA GLN AMIDOTRANSFERASE SUBUNIT B MITOCHONDRIAL AND PROKARYOTIC PET112-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659:SF2">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02938">
        <property type="entry name" value="GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55261">
        <property type="entry name" value="GAD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="GatB/YqeY motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Asn/Gln amidotransferase" evidence="7">
        <location>
            <begin position="489"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH35205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="640" mass="71605" checksum="83009A6A02709978" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUS9</accession>
    <name>A0A2S2KUS9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase C39-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_20260</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877844.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUS9"/>
    <dbReference type="GeneID" id="76210261"/>
    <dbReference type="OrthoDB" id="4906at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039564">
        <property type="entry name" value="Peptidase_C39-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13529">
        <property type="entry name" value="Peptidase_C39_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13174">
        <property type="entry name" value="TPR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Peptidase C39-like" evidence="4">
        <location>
            <begin position="13"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="238"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH35235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="319" mass="36304" checksum="2A0A8B89F36C7901" modified="2018-07-18" version="1">MVQDHELLLPLVEEENICLPLPINVVSKYWNIELPMAEAIETAKKYSGFNGSILVEGIESAERHGLTCKIINSSLSELKKIIDSGIPPIVILPGIPEITQHASVITGYDDEEKTILHYIQKGNQEGEQQEGAIPEEIFEKEWSEEGRLLILLAPPDILSAVKLENDSSDKSNRLCIMAERENILKNSSDALESLNKALQIYPNNSTALHQLGSMMNEQNSSECVKFYEKCLDINKRSYLTYNGLGNFYLKTNQFEKAENYYTKAIEINPKRSAKIYKNRAYLREKQNKNSEAKDDLRSYLKYYPKAPDRGVIEQAIREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNM6</accession>
    <name>A0A2S2KNM6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Snf7 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_00140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875898.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNM6"/>
    <dbReference type="GeneID" id="76209340"/>
    <dbReference type="OrthoDB" id="2392at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="32"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="23838" checksum="DBF643E1AD1DFEAA" modified="2018-07-18" version="1">MISNSWNKAERESISQKVMGRVKPDEPLKNKIDFAQKKLQFQISKLESINEKLQKKHDIIFEKIVNAQRNNKPSYAQAYAGELTQVRKMKNMVSGAKLSMEQVKLRLDTVSELGDVVVTLSPCMSIIKGLSPSLNGIMPEANASMQDLSQILGDVMSGSSVNMGDAMNMGTETNADTLAILEEAHNVIAGQTKSSIPDVPDSLKKQIVERKSDIFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNP2</accession>
    <name>A0A2S2KNP2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Biotin synthase</fullName>
            <ecNumber evidence="4 13">2.8.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13 16" type="primary">bioB</name>
        <name evidence="16" type="ORF">NZNM25_00100</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(4R,5S)-dethiobiotin + (sulfur carrier)-SH + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = (sulfur carrier)-H + biotin + 2 5'-deoxyadenosine + 2 L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:22060"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:149473"/>
            <dbReference type="EC" id="2.8.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="13">Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="14">Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13 14">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="13 14">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 13">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the radical SAM superfamily. Biotin synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.6" evidence="4 13"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875894.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNP2"/>
    <dbReference type="GeneID" id="76209336"/>
    <dbReference type="OrthoDB" id="9264at2157"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00162"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004076">
        <property type="term" value="F:biotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000026">
        <property type="entry name" value="Biotin synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01694">
        <property type="entry name" value="BioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002684">
        <property type="entry name" value="Biotin_synth/BioAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024177">
        <property type="entry name" value="Biotin_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00433">
        <property type="entry name" value="bioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976">
        <property type="entry name" value="BIOTIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976:SF2">
        <property type="entry name" value="BIOTIN SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06968">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001619">
        <property type="entry name" value="Biotin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01060">
        <property type="entry name" value="BATS_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01278">
        <property type="entry name" value="biotin_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13 14" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5 13 14" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10 13" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="11 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12 13 14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13 14" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Radical SAM core" evidence="15">
        <location>
            <begin position="50"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001619-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GBH33219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="325" mass="36238" checksum="06BDA5827AB9C8FF" modified="2018-07-18" version="1">MSAYEFIKECQEKVFSGISISSEDAKKLLNIQDEKLKELAKCANEITREFNGNKVDVEQLNNIKKNACSEDCTFCGQSAFFDTGIDTYQLPSPEDVVSKAQKAKEDGAESYCLVAAWREPTTRDFEKVCKIISEINDKVGINVECSLGFLTPEQAKKLKELKVKRYNHNLETAKSKFPEICTTHTYEDRLKTLKIARDAGLELCTGGIIGLGETREQRLELALELGRLYPEEVTINILVPMPGTPLELQTDLPNSEIVRIFSVIRFLLPESVIKISGGRESKLEDSGEELLQSGANGIITEGYLTMGGNEAKKDRELIEKIGLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNP9</accession>
    <name>A0A2S2KNP9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875942.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNP9"/>
    <dbReference type="GeneID" id="76209393"/>
    <dbReference type="OrthoDB" id="1614at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="71" mass="8314" checksum="6441544245A1793E" modified="2018-07-18" version="1">MNEQEQLMDNLLNVDLEIIDVVRELHQGNWDSQSLKHQVGDLLKIRDEMVQKLMSAKGDHHQCDCDHDHSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNR2</accession>
    <name>A0A2S2KNR2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4352 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_00400</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875923.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNR2"/>
    <dbReference type="GeneID" id="76209370"/>
    <dbReference type="OrthoDB" id="4917at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029051">
        <property type="entry name" value="DUF4352"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029050">
        <property type="entry name" value="Immunoprotect_excell_Ig-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11611">
        <property type="entry name" value="DUF4352"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4352" evidence="3">
        <location>
            <begin position="37"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="161" mass="17992" checksum="771E3DCBB00E3950" modified="2018-07-18" version="1">MAGMGVIIVIGAIIASMVVANYMYIQYTTNYIEAVAGKPIMVGPVEYAITFEGTHEGSKEMMPENTFVKIGITAKNTSDEKTILSGGQFYIVDEKDQKHEAVFGEFSSKDLFLESLEPNKSIERTTQFDIPFDEEKKYKIIIRPQKDQSTVDTAVVCIRNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNS6</accession>
    <name>A0A2S2KNS6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Caffeoyl-CoA O-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_00770</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNS6"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050362">
        <property type="entry name" value="Cation-dep_OMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002935">
        <property type="entry name" value="SAM_O-MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10509:SF14">
        <property type="entry name" value="CAFFEOYL-COA O-METHYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10509">
        <property type="entry name" value="O-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01596">
        <property type="entry name" value="Methyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51682">
        <property type="entry name" value="SAM_OMT_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="211" mass="24224" checksum="B41BBDBF863FCCA5" modified="2018-07-18" version="1">MKPYKSMKESIFNTLNELEVQSTLEKSRMVDTLPENRMLAITKETGELLNMLLRLKNAKNMLEIGTSVGYSSIWCAEAILEQAGKIITIEQNPNKVKRAKENFLKAEIQDAVEIKEGHALDILSKMSCIDEYKEYFDFVLIDADKENIIEYFDLILPMVSVGGIIVTDNMLYPEKYREHMKKFSDYLKENPDLNTITSNIGNGEEITIKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP67</accession>
    <name>A0A2S2KP67_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_01750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876256.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP67"/>
    <dbReference type="GeneID" id="76209507"/>
    <dbReference type="OrthoDB" id="2830at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052048">
        <property type="entry name" value="ST_Response_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228:SF1">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ARR22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="1">
        <location>
            <begin position="8"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="195" mass="22082" checksum="8A15B6BE610B1038" modified="2018-07-18" version="1">MEPLRYCKILIVDDSAAFREKIRYVLTDAEIGYYYYEAVDGREAVSQYITNKPDVVIMDLMMPNVDGLSAIKAIKKYDPDAKIIVASTRENKELVQDSIKGGVKDYIIKPFQPGAVVMAVSKALVATKEYKEKKAKPVTFIDKPLLYKGVYHGHVVNVSEYGEYELSANQDPLTKKLLQKAHDIKDMGKHYELVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPA1</accession>
    <name>A0A2S2KPA1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_01880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146195960.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPA1"/>
    <dbReference type="GeneID" id="76209519"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="77" mass="8681" checksum="E886402F1DE70AA9" modified="2018-07-18" version="1">MKTRIKIIFILVIVVGLLLAANFTVYEEGDFDSLQDEVVIVCDINWLQKPINCKPVFPEGFDFNAGTGFITSESNWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPA3</accession>
    <name>A0A2S2KPA3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_01420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876012.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPA3"/>
    <dbReference type="GeneID" id="76209472"/>
    <dbReference type="OrthoDB" id="342253at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAS" evidence="1">
        <location>
            <begin position="2"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="175" mass="20299" checksum="0C29DF61B372B9C0" modified="2018-07-18" version="1">MPQTEARKTLKDAPVMWRRINSIGIILDCNSTYAANLGYAKSEILGRSIFEHVVKDSWEAMNESLKSWFETGKVTDRKITFKRQDGSTFPGLLQATSIYDENNNLLGSNTVIFDLTQMNSEKIKEYEEFFKDAKNRLDEIKEKEYDQLDENSKSEYDGLKKMFEMLLEVNLAELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPC5</accession>
    <name>A0A2S2KPC5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875983.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPC5"/>
    <dbReference type="GeneID" id="76209438"/>
    <dbReference type="OrthoDB" id="1055at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="124" mass="14545" checksum="4D814F2BF273E6D9" modified="2018-07-18" version="1">MSISGMYMLNAEEYKEEKIQQALDMLYVDRKNEFRELSHVLLSEKALKSMPNWKEFVLNFSLDVESAFKTWSGRSPLSVKSPQKALTILRQLGHEKTSMNQLAHLLNISYNLSLEFKEIYKRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPF2</accession>
    <name>A0A2S2KPF2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03000</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_160049187.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPF2"/>
    <dbReference type="GeneID" id="76209637"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="58" mass="6279" checksum="60AB47331E99E5EA" modified="2018-07-18" version="1">MSNEKNKQTELESIKNLLILLLIKSGATSSEIATCLGVDSSGIRKKFPTRKNKSKGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPH1</accession>
    <name>A0A2S2KPH1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876028.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPH1"/>
    <dbReference type="GeneID" id="76209489"/>
    <dbReference type="OrthoDB" id="2829at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="586" mass="69416" checksum="A58F58687197D978" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPI2</accession>
    <name>A0A2S2KPI2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Inosine-5-monophosphate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_03300</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876179.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPI2"/>
    <dbReference type="GeneID" id="76209668"/>
    <dbReference type="OrthoDB" id="43333at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02205">
        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="7"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="73"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="135"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="227"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="281" mass="31722" checksum="6CA756CD71A754EF" modified="2018-07-18" version="1">MKYAKDFMNTPRTIKYESNLADVLKKIIDEKKSRLLVTQNGKIIGLISEKDLGIFLLTDTTERKLDEIPLSEIIKKIISVDEKTGMDKCAQLMMTNEIGSLVVTSNDEVVGILTKTDLVRYFTKLDSQKIVGEYMSPYYAWQYYDTPLYKVVLKMVDEKISRVILRDHEETPVGIVTFRDLFNLALTLGDKEDVLDNTDPLISVIFPRKGFISESGFGGSTQINEIMTKNIVSVNYDDDLTKAAKLILEKNINGVAVLSVHRNIIGIISKTDIVRALAFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPI4</accession>
    <name>A0A2S2KPI4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Peptide-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_02680</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876126.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPI4"/>
    <dbReference type="GeneID" id="76209603"/>
    <dbReference type="OrthoDB" id="194307at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904680">
        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.105.10">
        <property type="entry name" value="Dipeptide-binding Protein, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290:SF9">
        <property type="entry name" value="OLIGOPEPTIDE-BINDING PROTEIN APPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PERIPLASMIC BINDING COMPONENT OF ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="796"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="domain" description="Solute-binding protein family 5" evidence="5">
        <location>
            <begin position="15"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="821" mass="94246" checksum="7644B657B3CBDE5E" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPM7</accession>
    <name>A0A2S2KPM7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DedA family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_03940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPM7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="21127" checksum="E296804EBE7E74E0" modified="2018-07-18" version="1">MLIGVNTAPILMPPSWIVLTSFYLLDPSLNVLFLAMVGASGATLGRFFLKKISGLFRNFVGEEQKTNLDIIGDYLNRKKYGYVIASFLFGATPLPSNMLFIAYGLMRAKSISIYAGFWFGRTISYVIMIYFGDAVLRPFFEIFEDRLTGILLIDGAGIGAIILFASINWTVLITQKKIKFVKPKIWRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPP5</accession>
    <name>A0A2S2KPP5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PhoU family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_04140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876293.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPP5"/>
    <dbReference type="GeneID" id="76209762"/>
    <dbReference type="OrthoDB" id="7738at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PHOSPHATE-SPECIFIC TRANSPORT SYSTEM ACCESSORY PROTEIN PHOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930:SF3">
        <property type="entry name" value="PHOSPHATE-SPECIFIC TRANSPORT SYSTEM ACCESSORY PROTEIN PHOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109755">
        <property type="entry name" value="PhoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PhoU" evidence="1">
        <location>
            <begin position="18"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="PhoU" evidence="1">
        <location>
            <begin position="122"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="219" mass="25167" checksum="FA137F297E7C77D4" modified="2018-07-18" version="1">MTRLIDPSLQKLSFIMGEMGDMVVESISLAIDSYLDGTNTIDKVHSLSNSIRSKYYEVEDLTFDMLLKYQPVADDFRFIRSSTEISYAFSRFGRYAYDITQVRDLFGDVSECTNASLVESTKKVKHMIKEAVLSFAELDVRKAIKIREDEKVIDRIYKERLPKLIESNNTKCALAEALLLRYLERIGDHAVFMSDAINYIVTGKHRPSEERIASHTKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPR2</accession>
    <name>A0A2S2KPR2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04340</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPR2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="62" mass="7203" checksum="D6E87594D753CFE7" modified="2018-07-18" version="1">MSLSSEDINKIKEKIMSEDDTKIQNNRLINRVVLINTCTNCGNRFKPVNSMIWETLCDLCIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPS6</accession>
    <name>A0A2S2KPS6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NAD kinase</fullName>
            <ecNumber evidence="8">2.7.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">ATP-dependent NAD kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nadK</name>
        <name evidence="9" type="ORF">NZNM25_04570</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>NAD(+) + ATP = ADP + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18629"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the NAD kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="8"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876327.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPS6"/>
    <dbReference type="GeneID" id="76209809"/>
    <dbReference type="OrthoDB" id="77798at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.30:FF:000020">
        <property type="entry name" value="NAD kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF43">
        <property type="entry name" value="BIFUNCTIONAL NADP PHOSPHATASE_NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 8" id="KW-0520">NAD</keyword>
    <keyword evidence="6 8" id="KW-0521">NADP</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="69"/>
            <end position="70"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="139"/>
            <end position="140"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="179"/>
            <end position="184"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="271" mass="29857" checksum="4F1835B6B2816D54" modified="2018-07-18" version="1">MKLQKVAVVSKVGSKDSEKAAREVAKKFLAKKSTVYTISPIEVEGAEKIEDLSELKKIKLDLVITLGGDGTTLRVFRNLQNETPILTINVGGNRGILAEITIEEIDNAINEILKDNFFLDKRIRVVASCGGVEFPPALNEIYISRVNLTKTAEIEIKFQNDTVKQKMDGVIIATPSGSTGHSFSLGGPILHESLNVLIITPVAPVYRLASIVVPDEKIEIICSHDCNIAMDAQVIKTVGYEEPIIIKKYNKPAVFIRLKKRGLRQMSKLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPY2</accession>
    <name>A0A2S2KPY2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 13 16">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="5 13 16">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">pgk</name>
        <name evidence="17" type="ORF">NZNM25_03980</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13 16">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="5 13 16"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_263970051.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPY2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00185"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000006">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000012">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="entry name" value="Phosphoglycerate kinase, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015911">
        <property type="entry name" value="Phosphoglycerate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406:SF23">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00111">
        <property type="entry name" value="PGLYCERATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 13" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 13 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13 16" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="13 14">
        <location>
            <begin position="21"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <begin position="59"/>
            <end position="62"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 15">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="351"/>
            <end position="354"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GBH33607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="402" mass="43975" checksum="EBD510C1040AC600" modified="2018-07-18" version="1">MKVLTLDDFDLKGKTVFLRVDMNCPIDPDSMEISGTKRIEEAIETLQSLKEAKVVVASHQGRVGNDDYTGMDKHAKVLEKLMGKKIKYVEDTIGEAAQNAIRNLHDGEILLLDNLRLCAEENYEFTPENAAKTIMVTRLSKLFDLCVLDSFPSAHRSHPSIVGFPQVLPACAGRIVEREVRNLDEIMTVAKAPHVIVLGGSKVPDRLEAIKLLIQNGRADHVLLTGVIANVFMRAQARIKSPLGIKREDEVVSKAHALIGEYPDVFATPVDIAIDKDGARVEMDVREIAKTDKIYDLGPKTVEYYSKLIAGAGTVFISGPAGFFEKENFSYGTKALLDSVANSMATTIVSGGHLTSVLKKLGLAEKIDHISTAGGALVLYLTGEKLPMIKALEDAAMKYRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ14</accession>
    <name>A0A2S2KQ14_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl11</name>
        <name evidence="10" type="ORF">NZNM25_05300</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the ribosomal stalk of the 50S ribosomal subunit. Interacts with L10 and the large rRNA to form the base of the stalk. L10 forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876391.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ14"/>
    <dbReference type="GeneID" id="76209881"/>
    <dbReference type="OrthoDB" id="8842at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1550.10:FF:000007">
        <property type="entry name" value="50S ribosomal protein L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="entry name" value="Ribosomal protein L11/L12, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_uL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_uL11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_uL11_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002232">
        <property type="entry name" value="PRK01143.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="Ribosomal L11/L12e N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL11 N-terminal" evidence="9">
        <location>
            <begin position="9"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL11 C-terminal" evidence="8">
        <location>
            <begin position="70"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH33739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="159" mass="16417" checksum="4D6629DCAAB90200" modified="2018-07-18" version="1">MGEQKVSSLVTGGGASAGPPLGPALGPLGVNIMEVIQAINDKTKDFEGMKVPVTVIVNTDTKKYDIEIGIPSAAALIMKEAGIQKGSGASGSTWVGDVTLDSVIKVANTKLEKSYASSLKSVAKTIIGTCLALGVKVEGKTPKEITAEINEGKWDAKFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ28</accession>
    <name>A0A2S2KQ28_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14 15">Shikimate kinase</fullName>
            <shortName evidence="14">SK</shortName>
            <ecNumber evidence="4 14 15">2.7.1.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">aroK</name>
        <name evidence="18" type="ORF">NZNM25_03490</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>shikimate + ATP = 3-phosphoshikimate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13121"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.71"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the GHMP kinase family. Archaeal shikimate kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.71" evidence="4 14 15"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876197.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ28"/>
    <dbReference type="GeneID" id="76209689"/>
    <dbReference type="OrthoDB" id="9602at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00088"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004765">
        <property type="term" value="F:shikimate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00370">
        <property type="entry name" value="Shik_kinase_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010189">
        <property type="entry name" value="SK_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01920">
        <property type="entry name" value="Shik_kin_archae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861">
        <property type="entry name" value="HOMOSERINE/4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF3">
        <property type="entry name" value="SHIKIMATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005758">
        <property type="entry name" value="Shikimt_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12 14" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="11 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 14 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="16">
        <location>
            <begin position="58"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="17">
        <location>
            <begin position="224"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="NCBIfam" id="TIGR01920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="GBH33558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="284" mass="30409" checksum="1DC04DF71B4A0A81" modified="2018-07-18" version="1">MAKVKATVHGAVSLVNAIATQKGATLGIELKVEALVETSSGKGIVIESDNKMLSSRLINKTVEKIVSNKDLEQNKISIRLDSEIPTGYGLKSSSAISSAVALACAKIFKPKMTDQQILLAGVDASIESKVSITGAYDDACSCYYGGFNVTDNAKKRRIHFEKGPSNLIAVIFIPKNRKRGNIKNLKVLSAVFDNAWKLAKKSNYWEAMIINGLATASILNSDPKIITNLIEKGALAASVSGNGPSIAAIVKKENEANIKKIFATLEGNVLVSKINNKKAEVYEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ33</accession>
    <name>A0A2S2KQ33_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">50S ribosomal protein L16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_04480</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876319.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ33"/>
    <dbReference type="GeneID" id="76209799"/>
    <dbReference type="OrthoDB" id="30538at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="entry name" value="Ribosomal protein L10e/L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047873">
        <property type="entry name" value="Ribosomal_uL16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018255">
        <property type="entry name" value="Ribosomal_uL16_CS_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_uL16_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001197">
        <property type="entry name" value="Ribosomal_uL16_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_uL16_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003239">
        <property type="entry name" value="PRK04199.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11726">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005590">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="Ribosomal protein L16p/L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01257">
        <property type="entry name" value="RIBOSOMAL_L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="169" mass="18973" checksum="0C0678008FB82718" modified="2018-07-18" version="1">MHGANYRTATGQIFTRKEYIKGKPQIKIAKFQGGKRGVYKYCVQLLINEKIQIRHMAIESARLAANKTLEKTTGETGYYSRLRIYPHNLLRENKQIATAGADRVSEGMRRSWGKAVSLGARVKQGQCIMEMYVNNEAHLEAAKKALHGACVKLPGTPTINVIEWNKVSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ65</accession>
    <name>A0A2S2KQ65_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">General stress protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_03860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876231.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ65"/>
    <dbReference type="GeneID" id="76209728"/>
    <dbReference type="OrthoDB" id="28487at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19086">
        <property type="entry name" value="AKR_AKR11C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053135">
        <property type="entry name" value="AKR2_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43312">
        <property type="entry name" value="D-THREO-ALDOSE 1-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43312:SF1">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="332" mass="37876" checksum="91A5C89DFAA6667E" modified="2018-07-18" version="1">MNYNKLGKTDIKVSELGFGAWSIALDWWGKKIEEDEAKRMLKKAYDLGINFFETGDMYGKGKSERLIGEVFKGMREEIVISTKYGYDFSEVEQIGHSELPQRFDEDFTRKALRDSLERLQTDYLDMYGLHNPKLKHIRDDSIFNVLDSFIENETIKTYQVALGPAIGWTQEGLEAMERPNVSAVQTVYNILEQTPGNELMQKAEEKNVGILVRVPEASGILTGKVNADTKINEKDHRSVRKGEWIKASLEKVEILRPIAERNGLTITELSMKFIMSKKGFASVFPTVVSEEEIVNYVEMTKGGYIPASDMKEIEELYNSWPSYELKATPQAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQA9</accession>
    <name>A0A2S2KQA9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_05020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876366.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQA9"/>
    <dbReference type="GeneID" id="76209854"/>
    <dbReference type="OrthoDB" id="379157at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="192" mass="21585" checksum="8F8CCADC4DE7A1E8" modified="2018-07-18" version="1">MRKTFEKRLNTANLRKRIIIVILGIFASYFGIIPLGLLYHKLSYAPLPDPVSSYFFFNSVRIGIMVLFLYLALVYVKPSLVPQQEKYRKLVLISILIIVTLTVALPSGYVIPQLEQGGCITKSGSYNGDDNFSGSSSQGITTESECVDNCIFSGKFNTREEKFCEFKGIFGKTHWVKSPDDFDTPVFGEIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQE2</accession>
    <name>A0A2S2KQE2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Replication factor C small subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Clamp loader small subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rfc</name>
        <name evidence="8" type="ORF">NZNM25_06830</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the activator 1 small subunits family. RfcS subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876521.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQE2"/>
    <dbReference type="GeneID" id="76210043"/>
    <dbReference type="OrthoDB" id="7928at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000952">
        <property type="entry name" value="Replication factor C subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001679">
        <property type="entry name" value="PRK00440.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF20">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="38"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="316" mass="35172" checksum="541C6F87B1B2C588" modified="2018-07-18" version="1">MLGSGMWVEKYRPTKLSEIVNQTEIIGSLEALIKDPTDMPHLMFSGSAGVGKTTAALCIANQILGDNFKDYTLELNASDERGIGMVREKVKKFSRFAGMGEVPFKIIILDEADEMTADAQTALRRIIEDTAKICRFIFIANNISKIIDPIQSRCATFKFTTIPEEDLIKRLEEIAKNEKIKFDKKGLKAIYDYSEGDMRHAINLMQATASLGGITEEHVKGSAGLTKTSDVDEVLKIALLGKVAEAREKMIELIKVYGMSESDFLKYLNSAVFKSKHEKLSDILEIIAKYDYRVLVGANSEIQLSAMLAELGKIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQF2</accession>
    <name>A0A2S2KQF2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_06930</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQF2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="122" mass="14163" checksum="24943746392F60BB" modified="2018-07-18" version="1">MLTALGIDLERQDINMVVDLLKPVIKDNVMFMKGKYDLKRCIEALEDYMRASGMKSDHRIEGSLHHFIIQHGLGMNWSLLTEQLLKEIFHEFLPEKNVKAQITETTVITTISLGEDFNEHEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQG9</accession>
    <name>A0A2S2KQG9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">D-glycerate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_04860</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQG9"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016618">
        <property type="term" value="F:hydroxypyruvate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05301">
        <property type="entry name" value="GDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000203">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase (SerA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF283">
        <property type="entry name" value="GLYOXYLATE_HYDROXYPYRUVATE REDUCTASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00670">
        <property type="entry name" value="D_2_HYDROXYACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="5">
        <location>
            <begin position="4"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="6">
        <location>
            <begin position="108"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="321" mass="36123" checksum="7D047DEE0456DD7D" modified="2018-07-18" version="1">MKKVFLTRTLHDFALNELKKKYQIEVHSGKIPISQTKLRSKIKDVDGIICFPYDKINKETIELAKNLKVISTYSVGFDHIDVNYAKERKIRVGYTPEVLTDATADLAFSLLLDLLRRVSEGDRIIRNGKWKVIYGAHDYVGVDLQGKTLGILGLGRIGKTLAKRAKAFEMNIIYHNRKPLSKSKENALGVKFASFEKIITQSDIISIHVPHTKETDQLFDMKVFNKMKKSAFLINTARGKIVNEKELVKALKKKIISGAGLDVFEQEPIGVKNPLTRLENVVLAPHIGSSTKETRAKMAEITVKNLNLGMNGKKPIYSVGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQH3</accession>
    <name>A0A2S2KQH3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bifunctional (P)ppGpp synthetase/guanosine-3',5'-bis(Diphosphate) 3'-pyrophosphohydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876537.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQH3"/>
    <dbReference type="GeneID" id="76210065"/>
    <dbReference type="OrthoDB" id="120252at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008893">
        <property type="term" value="F:guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052194">
        <property type="entry name" value="MESH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46246">
        <property type="entry name" value="GUANOSINE-3',5'-BIS(DIPHOSPHATE) 3'-PYROPHOSPHOHYDROLASE MESH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46246:SF1">
        <property type="entry name" value="GUANOSINE-3',5'-BIS(DIPHOSPHATE) 3'-PYROPHOSPHOHYDROLASE MESH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13328">
        <property type="entry name" value="HD_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="21442" checksum="A572E3291B2EC83F" modified="2018-07-18" version="1">MELVKNAELFAKAKHSGQYRKDSNAPYSKHLDDVVNRLKSLGVIDQEILSAGWLHDIIEETDTNFDDLYEQFGNRIAVIVSSLSKNKTLPRKKREKEYSIQLREASFDAKLIKLCDISANLSDLKNYETSKSKKIRQAKQKRHYLTIIKTDLTNSNYPKIATLLETINQILKRYGLKSVSLQPELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQH7</accession>
    <name>A0A2S2KQH7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_07070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876574.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQH7"/>
    <dbReference type="GeneID" id="76208968"/>
    <dbReference type="OrthoDB" id="135845at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002774">
        <property type="entry name" value="Flagellin_arc-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01917">
        <property type="entry name" value="Flagellin_arch-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="158" mass="16958" checksum="95AB56E780C6DABD" modified="2018-07-18" version="1">MASGMISEGILIIASIVVAGLITGIVVSKVGTFEGYYTATTEAQKDKMLSKFEIIHVSQLNNTSVNAWIKNTGLTPIKGLGSSDLYFGPITSVDFVEYSTGTTPQWIFNGTNVDSVWSQGDTIQITIQDGTALQSNTSYLVQLTISNGESDDYIFSVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQJ9</accession>
    <name>A0A2S2KQJ9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">YncE family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQJ9"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011048">
        <property type="entry name" value="Haem_d1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051200">
        <property type="entry name" value="Host-pathogen_enzymatic-act"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47197:SF3">
        <property type="entry name" value="DIHYDRO-HEME D1 DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47197">
        <property type="entry name" value="PROTEIN NIRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51004">
        <property type="entry name" value="C-terminal (heme d1) domain of cytochrome cd1-nitrite reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="461" mass="51474" checksum="2EF9F913D8477219" modified="2018-07-18" version="1">MSLNAYRLFIISSAFFGLVALSGNAFAIQDTVKVGDFPRDIDFDPKLNNLYVPNYESGTISIIDASTMIIKDTVIINEGNSNPTRIAIDYKQHLIYVTDKISGILSVIDGINGKVIKSIKIGESLWDIALNENNKKLYISDLIKNEIIILDTENFEVIKSIFINSSPWAITINQNTDKTYVASGTSEAIHIIDGNTDELINDINPGVKPWGLSINEKTNVLYITSWDSNAITVLDLQNEQVIFEIPITSGAWQMNTNQNNGITTISNEHSNELYLLDENSKKIQTISVFDSPQSMIISPTANIIYVANPLSNSISAVSYDYDHYTLTPIIEEVISNNNSVNDELILEVIEGISNIPQREDIDTDMISGLLKNLGVTGEFDGNGIARILLDDYNKKKELQPKTAVIPTWTMDLAMMFSNDLENKPQPKEINCDEEFFFPINDIDKVNPYEIWVNILPICALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQK1</accession>
    <name>A0A2S2KQK1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">30S ribosomal protein S5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">NZNM25_05160</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit. Contacts protein S4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876378.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQK1"/>
    <dbReference type="GeneID" id="76209867"/>
    <dbReference type="OrthoDB" id="38155at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000004">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_uS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047866">
        <property type="entry name" value="Ribosomal_uS5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_uS5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005711">
        <property type="entry name" value="Ribosomal_uS5_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_uS5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_uS5_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003125">
        <property type="entry name" value="PRK04044.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01020">
        <property type="entry name" value="uS5_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718:SF4">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="RIBOSOMAL S SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 9 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="S5 DRBM" evidence="12">
        <location>
            <begin position="89"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH33725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="240" mass="25620" checksum="A9274B93F8E1D3E1" modified="2018-07-18" version="1">MSQTAQSKGGQGQRGRGAPVYGRGPSNGSKTGSDRPRRPRREPEEEVWVPKTILGQKVASGEITSLEEIIESGLRIQESGIIKKLLPDLKSEVVDVGIIQKMTSNGQSTRFKAIVATGNENGYLGIGQGKSKQMRIAIEKATSQAFLNVSPIKMGCGSWECKCEQKHSVPFKVKGKGGSVTIEIIPAPRGLGLVAGGKIKRLLELAGLKDAWTTAKGSTPTMNSTSKAILDCLRQTFSQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQL7</accession>
    <name>A0A2S2KQL7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein P1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl12</name>
        <name evidence="6" type="ORF">NZNM25_05390</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit. Homodimer, it forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which the L12 dimers bind in a sequential fashion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876401.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQL7"/>
    <dbReference type="GeneID" id="56066918"/>
    <dbReference type="OrthoDB" id="3337at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022295">
        <property type="entry name" value="Ribosomal_P1_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03685">
        <property type="entry name" value="ribo_P1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="67"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="71"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="98" mass="10009" checksum="59E407DD3BCF859B" modified="2018-07-18" version="1">MEYVYAALLLHKLDKEVNEANLTSVVKASGAEVNDAQVKALVAALADVNIDEAVKAAPVAVAAAAPAEAAGEAKKEEAPKDEGKTEEAAMEGLSSLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQR8</accession>
    <name>A0A2S2KQR8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF3426 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876669.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQR8"/>
    <dbReference type="GeneID" id="76208852"/>
    <dbReference type="OrthoDB" id="2701at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047676">
        <property type="entry name" value="FxLYD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038353">
        <property type="entry name" value="FxLYD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="252" mass="27803" checksum="523AF31C28B2F32B" modified="2018-07-18" version="1">MEKFFIVVFLFVGLIPAAFGEAFIENDQQFIGDDKSLHVVGEITNNLKVPLSQISVLITLLDEDKNPIAIREADSLVNTIMPGMKGPFELILTRNDAKNTESYSIELDYELSPPKNQVIDITESKLSTDNYNNLMITGTVMNKGDITANTVAIIATLYDIEGKVAAVSKVHPEPDYLGVNDNAFFLVTIPDKIQNNQIKEYTLVAESEEYAAVPEFPIGTLVVLVLTLSVYVGITRYSGRIITNLISVTNPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQU0</accession>
    <name>A0A2S2KQU0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_06060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876456.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQU0"/>
    <dbReference type="GeneID" id="76209959"/>
    <dbReference type="OrthoDB" id="43754at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43072:SF52">
        <property type="entry name" value="GCN5-RELATED N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43072">
        <property type="entry name" value="N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="258" mass="30668" checksum="8D55E9F45B01D4C0" modified="2018-07-18" version="1">MIREATIGDKIPILKFCKNTFSWGDYIEQVWDFWLSEGNLFVFEKQFPVGICHAFYFKDQIWIEGIRIDPEHRRQKIASKLVMHAEIVGKEKNASFSYMLIDIQNSSSLKMAHSLDYEIFQTWNFFSLLPKHNLNHKIQFEKSLNLKRYSHYVKSWRWLPLDDSTLSKFYDDKKIIKSTNLENDSIAILVDSEHFTKTLIVTLFSGSENSVLQLISFLQNYAMEKKYERIQILTKEYLPTFDTLEHKISFYLMKKCLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQU6</accession>
    <name>A0A2S2KQU6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF393 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_06160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876465.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQU6"/>
    <dbReference type="GeneID" id="76209970"/>
    <dbReference type="OrthoDB" id="6714at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="14351" checksum="937782C7DA93C95C" modified="2018-07-18" version="1">MEIIENVPIVVFDNQCYLCIKFAKAVDFFARGKISMVGHYSDFGKKIRDQILDESALEMFWFIDKKTAYGGRAAIYPLLKSFFSKRTKKLISVKIDDNCEQNCKTVNAVFVRSSSLLTNSKKINF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQW3</accession>
    <name>A0A2S2KQW3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07950</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876649.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQW3"/>
    <dbReference type="GeneID" id="76208881"/>
    <dbReference type="OrthoDB" id="4726at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="215" mass="25066" checksum="D4C30F4E6E0E84E0" modified="2018-07-18" version="1">MKDELIGLSIITVLGLFGSVTSSFAEDSLSWKSEYFVGILDSKTDEHIPVMVTTFSKDSLKLDWAKPEITTNQKIISYDVLRKDLNSDYYKIAEITNLKQSSYIDMNLNEGYYGYKIIPVLMKTDSDKITMHGINREHSFFPTYVQGQQLVAQNLLKQNCLKCFDDSFEEIDNIFQYEFSEYTKRTNNEYQQNLDSESLRTENLFAFLFKIRNNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQW9</accession>
    <name>A0A2S2KQW9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">YnfA family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_07480</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876608.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQW9"/>
    <dbReference type="GeneID" id="76208928"/>
    <dbReference type="OrthoDB" id="129688at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00010">
        <property type="entry name" value="UPF0060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003844">
        <property type="entry name" value="UPF0060"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002586">
        <property type="entry name" value="PRK02237.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36116">
        <property type="entry name" value="UPF0060 MEMBRANE PROTEIN YNFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36116:SF1">
        <property type="entry name" value="UPF0060 MEMBRANE PROTEIN YNFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02694">
        <property type="entry name" value="UPF0060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="39"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="121" mass="13778" checksum="5DF35B8791B4F0E4" modified="2018-07-18" version="1">MSEKSKNYLTSVLLFFLAGLCEIGGGYLVWLWLRDDFSWILGAISGFVLFLYGIVPTFQKTHFHRVYASYGGVFIVMSVFWGWLIDGITPDRYDIIGTVIAVIGVLIIFYYPRKEAKSCSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQY6</accession>
    <name>A0A2S2KQY6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08670</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866866.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQY6"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="241" mass="28134" checksum="A747C0BF99DEB5C4" modified="2018-07-18" version="1">MQEKSKVSPMAKKLDSTVQEVHRNFERLAKSGFIEKDVEGYYHLTVYGSTVCTQIPTMIFFSNNRKYFQEHDFGNLPAKFIERIGALSTGVYVKGFTKVMEKWKSIYKNANEYIYELHSEVSNDLIEPLIKKARDDKVKINYILSESCIVPKGRKRTLEKFGFNQLLENGIIERKMSKDVLVVVALNEKEACISFPNRNGDANIGFAFYGNDVRFHEWCLDYFRYCWYNSGSFRESKLKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR32</accession>
    <name>A0A2S2KR32_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">methionine synthase</fullName>
            <ecNumber evidence="5">2.1.1.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">5-methyltetrahydrofolate--homocysteine methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">NZNM25_09310</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>methylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:28115"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetH route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the vitamin-B12 dependent methionine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.13" evidence="5"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR32"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008705">
        <property type="term" value="F:methionine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050667">
        <property type="term" value="P:homocysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046653">
        <property type="term" value="P:tetrahydrofolate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.330:FF:000001">
        <property type="entry name" value="Methionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.330">
        <property type="entry name" value="Homocysteine-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017226">
        <property type="entry name" value="BHMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003726">
        <property type="entry name" value="HCY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036589">
        <property type="entry name" value="HCY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050554">
        <property type="entry name" value="Met_Synthase/Corrinoid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45833">
        <property type="entry name" value="METHIONINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45833:SF1">
        <property type="entry name" value="METHIONINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02574">
        <property type="entry name" value="S-methyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037505">
        <property type="entry name" value="Betaine_HMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82282">
        <property type="entry name" value="Homocysteine S-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50970">
        <property type="entry name" value="HCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="14" id="KW-0170">Cobalt</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Hcy-binding" evidence="17">
        <location>
            <begin position="1"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="GBH34140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="299" mass="32859" checksum="2B50249C2DCC74D9" modified="2018-07-18" version="1">MGTEIQKYDPKPEDFPNNQDGFNDGLVITHPEWIKQIHRNYLDAGSDCIETNSFGSNKIKLDEYGFGDQTVEFNKKIAKLACEVCEEYSDKPRYVIGSMGPSGYLPSSNDPDLGQKPLDEIREAFALQSEGLILGGVDALLIETSQDILEVKLVIEACHSAIKKTGKKVPIIANTTLDQYGKMLLGTDIQAAYTTVSDMGIDVFGLNCSTGPIEMSPSVRWLDEQKDHNILVVPNAGMPENEGGQAIYKMSPEKMGEALGDFLNQYHKVRIIGGCCGTNPEHIRALRKVIDEKANSVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR43</accession>
    <name>A0A2S2KR43_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">3-phosphoglycerate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_09170</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876752.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR43"/>
    <dbReference type="GeneID" id="76208753"/>
    <dbReference type="OrthoDB" id="7437at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05303">
        <property type="entry name" value="PGDH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000203">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase (SerA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050857">
        <property type="entry name" value="D-2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42789">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42789:SF1">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="6">
        <location>
            <begin position="7"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="7">
        <location>
            <begin position="109"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="310" mass="33742" checksum="1750EF82EC44C5D6" modified="2018-07-18" version="1">MGFDDSVLICDEIDPILNKILEDNGLKISYEPEITPEQIIEKISNFNIVIVRSRTTITKEMIDKADNCKIIARVGVGLDNVDQVAAKAKNIRVINAVEGAMNAVAELVLGLMLSLARQTARGDRAIRNGQWLKKELKGTELRGKYLGIIGLGNIGKRLGRLARALNMNIIGYDVIPIDEEFSKEVGLMKADLNTLLQSSDYVSIHVPLLDSTHHLLDAQKMSTMKNTAKIINTSRGGVVDEDALYDALKNGKLGGAALDVFEKEPAIGTKLAELDNVILTPHIGAQTKEAQSLAANVIGEKIIQILRGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR69</accession>
    <name>A0A2S2KR69_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Diphthine synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_09470</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the diphthine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876781.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR69"/>
    <dbReference type="GeneID" id="76208723"/>
    <dbReference type="OrthoDB" id="39139at2157"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004164">
        <property type="term" value="F:diphthine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11647">
        <property type="entry name" value="DHP5_DphB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.90">
        <property type="entry name" value="AF1782-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036809">
        <property type="entry name" value="AF1782-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004551">
        <property type="entry name" value="Dphthn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023140">
        <property type="entry name" value="DUF357"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00522">
        <property type="entry name" value="dph5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882:SF0">
        <property type="entry name" value="DIPHTHINE METHYL ESTER SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882">
        <property type="entry name" value="DIPHTHINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04010">
        <property type="entry name" value="DUF357"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158372">
        <property type="entry name" value="AF1782-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="DUF357" evidence="7">
        <location>
            <begin position="273"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="38845" checksum="5787D9A7A0C13B07" modified="2018-07-18" version="1">MLWFVGLGISGFKSIPAEALDVLSKADIVYMEQFTSPIGKSDLTKIKNATKGEFRPAKRWLVEDGNEILKNSKKKKVVLLSYGDPYIATTHIELRTRAIEEKIKTHSIHASSSLTSMIGECGLHFYKVGRIATIMSEMKSLTTPYYVIYKNIIEGNHTVLLLEYNQDKDFFLDPKDALDGLLETEKGQKRNVISSDSYAIIASRVGFKDQSIISGKISSLKKKDFGKPPHTVIIPGRLHFTESDALKILGQCIDEPFDNSEKTKKIPVQMMEKYVPMVREALKEVEPHYKDQKEYQAILENAELYINDAEKFLEDGQDEVAILSIGYADGLVDALRLAKGMDPKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR71</accession>
    <name>A0A2S2KR71_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12 13">UvrABC system protein B</fullName>
            <shortName evidence="12">Protein UvrB</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Excinuclease ABC subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">uvrB</name>
        <name evidence="17" type="ORF">NZNM25_07390</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10 12 13">Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">The beta-hairpin motif is involved in DNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12 13">Belongs to the UvrB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876600.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR71"/>
    <dbReference type="GeneID" id="76208937"/>
    <dbReference type="OrthoDB" id="8371at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120545">
        <property type="term" value="F:nucleic acid conformation isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd17916">
        <property type="entry name" value="DEXHc_UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18790">
        <property type="entry name" value="SF2_C_UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.10">
        <property type="entry name" value="UVR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00204">
        <property type="entry name" value="UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036876">
        <property type="entry name" value="UVR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004807">
        <property type="entry name" value="UvrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041471">
        <property type="entry name" value="UvrB_inter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024759">
        <property type="entry name" value="UvrB_YAD/RRR_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003673">
        <property type="entry name" value="PRK05298.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00631">
        <property type="entry name" value="uvrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24029">
        <property type="entry name" value="UVRABC SYSTEM PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24029:SF0">
        <property type="entry name" value="UVRABC SYSTEM PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12344">
        <property type="entry name" value="UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17757">
        <property type="entry name" value="UvrB_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46600">
        <property type="entry name" value="C-terminal UvrC-binding domain of UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12 13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 12 13" id="KW-0228">DNA excision</keyword>
    <keyword evidence="9 12 13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 12 13" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 13" id="KW-0742">SOS response</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="27"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="426"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="UVR" evidence="14">
        <location>
            <begin position="607"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Beta-hairpin" evidence="12">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GBH33948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="650" mass="74949" checksum="2F3ED8E0533C7D7F" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR77</accession>
    <name>A0A2S2KR77_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycerate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_07460</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR77"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008887">
        <property type="term" value="F:glycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10180">
        <property type="entry name" value="Glycerate kinase, MOFRL-like N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1480.10">
        <property type="entry name" value="MOFRL domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037035">
        <property type="entry name" value="GK-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038614">
        <property type="entry name" value="GK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007835">
        <property type="entry name" value="MOFRL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025286">
        <property type="entry name" value="MOFRL_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039760">
        <property type="entry name" value="MOFRL_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12227">
        <property type="entry name" value="GLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12227:SF0">
        <property type="entry name" value="GLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13660">
        <property type="entry name" value="DUF4147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05161">
        <property type="entry name" value="MOFRL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82544">
        <property type="entry name" value="GckA/TtuD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MOFRL-associated" evidence="2">
        <location>
            <begin position="10"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="MOFRL" evidence="1">
        <location>
            <begin position="311"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="417" mass="46375" checksum="42EB7C14FDA985D4" modified="2018-07-18" version="1">MATTEKKKDCLEILEAGLRAADPKNIITKYVTPNEIKIKGKVINIEKYSNIYSVAFGKAGDSMTRALNAIMPIKSGIIVIPKGSKAKIKGKKFQIFNSRHPQPDKTSVKAAKEVMKFVQNKRSDELIIFLVSGGGSSLLAMPDEITLDDKIHVTNLLLKAGVTIQEFNCIRKHLSKIKGGKLIENMKCHGISLVMSDVEGDDLSSIASGTTYMDDTTYEDALEIIDKYKIRWKIPTEVLQILEKRLNEKRCETPKKAQIENYIIANNNDCLQAMQKKADEIGYKVSIMQIFGDIKEVVPKILENISDEQNTCLIFGGEPTVKVLGKGMGGRNQELVLRLLKNTQKAKKIVIASMGTDGIDGNSIFAGAITENIKVDLDVMKEFLKNSDSGRFFQKQKGNIITNFTHTNLMDIGMILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRA9</accession>
    <name>A0A2S2KRA9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Proteasome endopeptidase complex,subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_09740</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876803.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRA9"/>
    <dbReference type="GeneID" id="76208695"/>
    <dbReference type="OrthoDB" id="9421at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000004">
        <property type="entry name" value="Proteasome subunit alpha type-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="5">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="241" mass="26327" checksum="0B46E64F70091D97" modified="2018-07-18" version="1">MMASRGYDMTPTMYSPDGRIYQVEYAIETVKRGTTAIGILSKEGVILAVEEKPRPLQTKNVTQKIFQVDYHIGVAAAGYIPDARVQVDSARFFSQGNRMTYDESVEVSTVAKHLADQAHQFTQYGGVRPNGVSMIIAGIDQKGGSIYVTDPSGTYLQYSAVAIGAGAEDVNEFLEKYYSKDMSLEDAAGLAIAAINLKAEQKDGASNVKMAKITDKTKVFEKVSESDLENYSKNASKFNTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRB5</accession>
    <name>A0A2S2KRB5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_10110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876832.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRB5"/>
    <dbReference type="GeneID" id="76208656"/>
    <dbReference type="OrthoDB" id="7794at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="77" mass="8751" checksum="F29D72CD599FB287" modified="2018-07-18" version="1">MEQSTIKLSKKIKNDLKKQMIHPGETYEMVIARLLKNVQEDDVLSPAIIKNIEEGVADIKAGRVYTSEQVKKRLGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRD3</accession>
    <name>A0A2S2KRD3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_10000</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876824.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRD3"/>
    <dbReference type="GeneID" id="76208667"/>
    <dbReference type="OrthoDB" id="11066at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="287" mass="33245" checksum="0A1FEC599747009E" modified="2018-07-18" version="1">MPSNEQGTVYGNLHAYKMFTRPTAQRLFGAYSFRKKSGTKHHENSQRLLEILALNGTLTTWGMAKTHINSSSAIRTKEKENRRLLVGRMARGKHTIGLLESGLVVKDGKSFAKAPADQYRLSLHGVLYCLDVLDLSDKQIDTMAEKYSSVLPMVFGKWDYLKSNVGNEVYKLKNLASGLFMDNVQISKISNFPVYELMTYLNVKYQQNFEQIEEEDLANQISIWFYTNLLVPTKFRTTGKHSSLEVKQWKKIIHGNDLIKTWYYDFVNEAIRFYNNRFTKLKKLEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRD8</accession>
    <name>A0A2S2KRD8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_09650</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876796.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRD8"/>
    <dbReference type="GeneID" id="76208705"/>
    <dbReference type="OrthoDB" id="8553at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002809">
        <property type="entry name" value="EMC3/TMCO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038978">
        <property type="entry name" value="MJ0935"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42198">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42198:SF1">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01956">
        <property type="entry name" value="EMC3_TMCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01415">
        <property type="entry name" value="DUF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="218" mass="24681" checksum="E01CB215F160D03C" modified="2018-07-18" version="1">MVEWLDHNAILLFLNSIFLQGDNGGIFGFLGQNRGALGSDDPIVKGLVPTLFGVTGFGIVLNFFNSTVRKKLVDQTKLNRIMKETRAWQKERMDAMRAKDQAKITQLNKKSSYMNKMSMEMMQMNMKPMMITFVPLILIFYLVLPHLFAHTVALSPISLNVFPGNFFHLTCTALQAMEEGNVCYGHENALYLWAWYFLSSISFSGIIMKVTKTSMGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRE7</accession>
    <name>A0A2S2KRE7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Kazal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRE7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16276">
        <property type="entry name" value="metallo-hydrolase-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025485">
        <property type="entry name" value="DUF4377"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050662">
        <property type="entry name" value="Sec-metab_biosynth-thioest"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23131">
        <property type="entry name" value="ENDORIBONUCLEASE LACTB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23131:SF4">
        <property type="entry name" value="METALLO-BETA-LACTAMASE SUPERFAMILY POTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14302">
        <property type="entry name" value="DUF4377"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00050">
        <property type="entry name" value="Kazal_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kazal-like" evidence="1">
        <location>
            <begin position="277"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="606" mass="67293" checksum="4B8F295DAF8690EC" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRH3</accession>
    <name>A0A2S2KRH3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dihydroorotate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_10630</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="1">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876874.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRH3"/>
    <dbReference type="GeneID" id="76208598"/>
    <dbReference type="OrthoDB" id="35401at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06220">
        <property type="entry name" value="DHOD_e_trans_like2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012165">
        <property type="entry name" value="Cyt_c3_hydrogenase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019480">
        <property type="entry name" value="Dihydroorotate_DH_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050353">
        <property type="entry name" value="PyrK_electron_transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000796">
        <property type="entry name" value="PRK00054.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43513">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE B (NAD(+)), ELECTRON TRANSFER SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43513:SF3">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE B (NAD(+)), ELECTRON TRANSFER SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10418">
        <property type="entry name" value="DHODB_Fe-S_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006816">
        <property type="entry name" value="Cyc3_hyd_g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00410">
        <property type="entry name" value="PHEHYDRXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="2">
        <location>
            <begin position="6"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR006816-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="270" mass="29609" checksum="E62E10A082FE7058" modified="2018-07-18" version="1">MQRNNNHTTIVTIEKVIDETPTVRTLVFSDDVMSNVLPGQFAMVWIPGVNELPMSVMISKESGKAAFTVRKHGPASTGLFNILEGQQIGIRGPYGNSFDLKEGKLLLVGGGTGLVPMMRLLTFVKPADDVTVLIGAKSKDEVFFEDLANNLLENNPHKVIVSTDDGSYGEKGFVTDLVEKLVSKSHFDGVYVCGPEIMMYKTVQSAHSRKMFVQASLERMMKCGVGICGSCCVGEDLVCRDGTVFDGQHLSTNKEFGHFYRNKAGILENY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRH7</accession>
    <name>A0A2S2KRH7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_08460</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRH7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08070">
        <property type="entry name" value="MPN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028090">
        <property type="entry name" value="JAB_dom_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051929">
        <property type="entry name" value="VirAsm_ModProt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34858">
        <property type="entry name" value="CYSO-CYSTEINE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34858:SF1">
        <property type="entry name" value="CYSO-CYSTEINE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14464">
        <property type="entry name" value="Prok-JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="6">
        <location>
            <begin position="14"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="139" mass="15975" checksum="A0DEFC347EED88BE" modified="2018-07-18" version="1">MDDRNSQRRIDLQKIIISESDKKILSEYSENQKPNESCAILFGKNDTVSDLFLTENIEESPVNFTISNEQLIEGYKMAEEKNLDVVGIFHSHPDSDAFPSNTDKKFMQSNPVVWIIYSGINKNFRAFILESDIIEIPIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRJ4</accession>
    <name>A0A2S2KRJ4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_10600</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866863.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRJ4"/>
    <dbReference type="GeneID" id="76208601"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032874">
        <property type="entry name" value="DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052183">
        <property type="entry name" value="IS_Transposase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036280">
        <property type="entry name" value="Multihaem_cyt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35528">
        <property type="entry name" value="BLL1675 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35528:SF3">
        <property type="entry name" value="BLL1675 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13610">
        <property type="entry name" value="DDE_Tnp_IS240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48695">
        <property type="entry name" value="Multiheme cytochromes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="26"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="189"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="43867" checksum="636299AD7DA3C4A4" modified="2018-07-18" version="1">MKNKREQKGKEIALKSDLIRVSDIHYKVHSQTSKRKYDVVKVGNSWTCNCADHKFRKVCCKHIHAIEISIKIRQEVRQQNKITIPSITISNCKFCHSDNVKKFGIRKNKSGNIQRFVCSDCHKTFSINLGFERMKSTPQSITSALQLYFTGESLRGVTKFLKLQGTNVSHVTVYNWITKYTELMQKYIDKIVPNVGDAWHADEIYMKIHGDLKYVFALMDQETRYWIAQEVADRKEGHDARGLLQKSKEVTQAKPKVFITDGLGSYHEAYKKEFWSLKREDRTLHIRHIHLQGDRNNNIQERLNGEIRDREKVMRGLKKMDTPILTGYQIYHNYLRPHMGLEGKTPAQACGIEIEGSNKWLTLIQNATDLNRSLIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRL2</accession>
    <name>A0A2S2KRL2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_10450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRL2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="71" mass="7588" checksum="AC7EC525502480EC" modified="2018-07-18" version="1">MFKHLCMSMDSKIALFSVLVLAAIFAMALPMDSVYAQTGDASTDGEDGEHEGKSCPSKDKKTAFIDSGLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRL8</accession>
    <name>A0A2S2KRL8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_09980</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876823.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRL8"/>
    <dbReference type="GeneID" id="76208669"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="95" mass="10763" checksum="20F8A37C6C31F48E" modified="2018-07-18" version="1">MRAFLIASIIVIMATILVFSMLLPAKAMKSDTSERLSPKSFGTKIKNDMCSVIFCKNITSVKTDHPSSHAKEDWQNKRLAQILVNYPKLMYLKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRP9</accession>
    <name>A0A2S2KRP9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Type II toxin-antitoxin system RelE/ParE family toxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_10120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876833.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRP9"/>
    <dbReference type="GeneID" id="76208655"/>
    <dbReference type="OrthoDB" id="97626at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2310.20">
        <property type="entry name" value="RelE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007712">
        <property type="entry name" value="RelE/ParE_toxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035093">
        <property type="entry name" value="RelE/ParE_toxin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35601">
        <property type="entry name" value="TOXIN RELE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35601:SF1">
        <property type="entry name" value="TOXIN RELE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05016">
        <property type="entry name" value="ParE_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143011">
        <property type="entry name" value="RelE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-1277">Toxin-antitoxin system</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="84" mass="10140" checksum="CE1AF585A0F2D153" modified="2018-07-18" version="1">MTWQVIWSEKSVKQLEKIDKKNAQRVYDAVLNCTRDPFKSMARLTNSPFYRLRMGNYRVILDLQQSKMIIFVIETDHRGRIYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRR6</accession>
    <name>A0A2S2KRR6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Orotidine 5'-phosphate decarboxylase</fullName>
            <ecNumber evidence="3">4.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">OMP decarboxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">NZNM25_10950</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>orotidine 5'-phosphate + H(+) = UMP + CO2</text>
            <dbReference type="Rhea" id="RHEA:11596"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="4.1.1.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OMP decarboxylase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.23" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877022.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRR6"/>
    <dbReference type="GeneID" id="76208563"/>
    <dbReference type="OrthoDB" id="94124at2157"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04725">
        <property type="entry name" value="OMP_decarboxylase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014732">
        <property type="entry name" value="OMPdecase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011995">
        <property type="entry name" value="OMPdecase_type-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01740">
        <property type="entry name" value="pyrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43375">
        <property type="entry name" value="OROTIDINE 5'-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43375:SF1">
        <property type="entry name" value="OROTIDINE 5'-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orotidine 5'-phosphate decarboxylase" evidence="12">
        <location>
            <begin position="17"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="10">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="10">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="10">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR614732-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR614732-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH34304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="246" mass="27064" checksum="8D29367F19234DD9" modified="2018-07-18" version="1">MVTFQTRLSQISKINGKVILANDYDRSVKNLETKTIQNIKQLHPFLCGIKLNFHLLLPLSAKAILKINNTAHRYGLQTIADIKLNDIGNTNRVTTEHLWNLGFDAVIANPIMGLDSLKNLVKSSHKSGKGVITLCHMSAPEARLSYDMEIKMGRKQQLYQLFLNWALAAKVDGIVVGATFPKIIQYCSKKAGKNLKIFSPGVGTQGGNANEVISSGTDYLIVGRTILNSKNPSSVAKELQLQSFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRT2</accession>
    <name>A0A2S2KRT2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="11">CoA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">NZNM25_09560</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876789.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRT2"/>
    <dbReference type="GeneID" id="76208714"/>
    <dbReference type="OrthoDB" id="18103at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000020">
        <property type="entry name" value="Protein lysine acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053579">
        <property type="entry name" value="3HP/4HB_CoA_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051538">
        <property type="entry name" value="Acyl-CoA_Synth/Transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032875">
        <property type="entry name" value="Succ_CoA_lig_flav_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045492">
        <property type="entry name" value="HydPropCoALig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43334:SF1">
        <property type="entry name" value="3-HYDROXYPROPIONATE--COA LIGASE [ADP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43334">
        <property type="entry name" value="ACETATE--COA LIGASE [ADP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13549">
        <property type="entry name" value="ATP-grasp_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13607">
        <property type="entry name" value="Succ_CoA_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="10">
        <location>
            <begin position="25"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="705" mass="76096" checksum="54688D7985360B4C" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRU1</accession>
    <name>A0A2S2KRU1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_10520</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRU1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035657">
        <property type="term" value="C:eRF1 methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052190">
        <property type="entry name" value="Euk-Arch_PrmC-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004557">
        <property type="entry name" value="PrmC-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00537">
        <property type="entry name" value="hemK_rel_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875:SF1">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase small" evidence="4">
        <location>
            <begin position="13"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="172" mass="19235" checksum="9A3407686CE3610C" modified="2018-07-18" version="1">MKNEEYPPSEDTFFIVKNIENEKGNFALDVGSGSGYLTKLLSENFTFVVGTDINFNVLKDQTYKTKNIVCCSGSDALKIKFDFIVCNLPYLATDEILDVATDGGTDGFEIPKKIFDSVINNIAENGKFVFVTSSLSDYQKLIHYAQKLGLQTRIVAKKKLFFEELILVEASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRW4</accession>
    <name>A0A2S2KRW4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_12110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRW4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="111" mass="12791" checksum="6A550EA498894B97" modified="2018-07-18" version="1">MNNIQSYKIFKNKANSCFSTFMAEKKTTKKTTKKDTKKIPSKKTKSKTTKIVKASKPKTKITKSKSKKSKEEPISDMGIVIVDDHIEIDQEKVNEERRAYLEEARSQEAFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRZ0</accession>
    <name>A0A2S2KRZ0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_10090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRZ0"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="194" mass="22882" checksum="FAF3F3A8426E81A1" modified="2018-07-18" version="1">MDNGPLTLYSANSKTGIPIGTIHRHFGQLSKSGKIRVYESKKKGRKKIGYGPTVYGIIKAYKQDREFAEKIENYFLIWIENKEFQKDLEKEGFDITIGNLKKSKHVFRKFLEYFSAVEEQIEKIRRGDDSTSRDIQVLFGSMMLSSDTHYQKLWAELYDQLPGIQKYLDEYMESMIKSYKEFKKHSREHNLKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS11</accession>
    <name>A0A2S2KS11_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_10360</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876855.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS11"/>
    <dbReference type="GeneID" id="76208629"/>
    <dbReference type="OrthoDB" id="6467at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861:SF6">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158997">
        <property type="entry name" value="Trm112p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="2">
        <location>
            <begin position="132"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="289" mass="33078" checksum="8E52F9D1E9E50DD1" modified="2018-07-18" version="1">MLEHTPEFLRCVGCGSKLELDTLQMKKEIEEGILKCKKCVLAFPIIEKIPILWNDFPKYLSHRKVLGGQLYQLSKTEKMKSFLKSSLSKTVHVDDRTAIDERWSKIYQNSMHSKFYSLIIRHLDSLPKPNLVLEHGCSIGIVTSSLADSSNTVFGIDRSFSALRFAKKTFKHNLDYVVADSLSPVFGKLQFDMVIALNLLELVEPLELLKHVSKQITFGYFVISDPYDFDRGVNSVKKSLDEFTLRANLEKFGFKIHANTKNPSYLPWNLKLNSRAILNYKVDLVIGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS18</accession>
    <name>A0A2S2KS18_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_10390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_179371267.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS18"/>
    <dbReference type="GeneID" id="56068471"/>
    <dbReference type="OrthoDB" id="7568at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="5"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="56" mass="6447" checksum="5C6AE220EE0A212A" modified="2018-07-18" version="1">MSSEAETIYERVAKLEDEIALLRSEVDILKKAFRNKIARHEISMIKKGNDIDSIID</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS19</accession>
    <name>A0A2S2KS19_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LLM class F420-dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_11950</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877314.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS19"/>
    <dbReference type="GeneID" id="76209311"/>
    <dbReference type="OrthoDB" id="194060at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01097">
        <property type="entry name" value="Tetrahydromethanopterin_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="entry name" value="Luciferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050564">
        <property type="entry name" value="F420-G6PD/mer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43244">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43244:SF1">
        <property type="entry name" value="5,10-METHYLENETETRAHYDROMETHANOPTERIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="Bacterial luciferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Luciferase-like" evidence="2">
        <location>
            <begin position="12"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="321" mass="36211" checksum="BA64E03F19FFE87F" modified="2018-07-18" version="1">MRIGFSLGSLLSVDEVLKCSEILSKTSVDTIWIPETWGMENFSMLSAVSNRTKSQKIGSSIINIYSRSPATIAMGAATVDVLSDGRLILGLGTSSLPIIEDFHGMEFIDPLKRMKEYVEIIRLILSGRVVNYNSSNFNLKNFTLLIKTKREFIPIYLAAINQKMVDLAWSIGDGVIFYLRPIEEMKKTISKMQTRNKIDVTCQIITSVSEDSDDSIIRAKKTLAFYISVGKIYRDFLASHGFKNETSAVYDEFKKSGFKSNHELISDSMLRSLVIAGTSEECRKQMKNFRNTGIDLPIIQFNPIGDVVKSFRLFKKTFLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS32</accession>
    <name>A0A2S2KS32_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ubiquinol-cytochrome c reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877027.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS32"/>
    <dbReference type="GeneID" id="76208514"/>
    <dbReference type="OrthoDB" id="35872at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd21133">
        <property type="entry name" value="EVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.590.10:FF:000009">
        <property type="entry name" value="EVE domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052181">
        <property type="entry name" value="5hmC_binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002740">
        <property type="entry name" value="EVE_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047197">
        <property type="entry name" value="THYN1-like_EVE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14087">
        <property type="entry name" value="THYMOCYTE NUCLEAR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14087:SF7">
        <property type="entry name" value="THYMOCYTE NUCLEAR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01878">
        <property type="entry name" value="EVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EVE" evidence="1">
        <location>
            <begin position="3"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="139" mass="16341" checksum="977649609A80B778" modified="2018-07-18" version="1">MVNYWLAKQEPSGPRGYNFEQLKKEKSTVWDGVHNNLALKHMREMKPGDLAFFYHTGDERQAVGIMQITSKPYSNPKEDVERFIVVDVKYKKSLKNPVTLDEMKKNKKFQDWELLRISRLSVMPVPKPIWDEILKMSQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS44</accession>
    <name>A0A2S2KS44_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator TrmB N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_12250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877072.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS44"/>
    <dbReference type="GeneID" id="76209279"/>
    <dbReference type="OrthoDB" id="12358at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051797">
        <property type="entry name" value="TrmB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="1">
        <location>
            <begin position="31"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="126" mass="14590" checksum="2AF90E35F15659DE" modified="2018-07-18" version="1">MGRINADKTHSSLGIENTINKLNLMIIDYYFTVEETKIFLFLNKNGVETASNISKILNIPRTETYRLLSALQRKGVVFSTFSKPTKFNAVRTEEVLEILSNKIRNKIAQIESETRRNHLTINSRWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS66</accession>
    <name>A0A2S2KS66_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_10960</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876945.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS66"/>
    <dbReference type="GeneID" id="76208562"/>
    <dbReference type="OrthoDB" id="6471at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="228" mass="26216" checksum="5EEF235E1A703E47" modified="2018-07-18" version="1">MDKLQTTTIDKFPRYIQVHSTLEFTRLVCALERAPRVSFLHDHNGKKILSVQMDILKEKPIVYYTPLEHNGHYLCYGLKGGKEESAIVDTTSDASKLYSPIVRIKSLPKTLQPGNGTLDRYQPIELEDLYSLAKLTWGFEEIPFPLFLFPRNDKWLIGVFMNFNDEGTSYFCHVVLDEDPDKPFLKFSTTDGTLPSFVENPSEHGYSYIKIIKLKDTHPLVDYGHLPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSA8</accession>
    <name>A0A2S2KSA8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HEAT repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13430</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877160.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSA8"/>
    <dbReference type="GeneID" id="76209156"/>
    <dbReference type="OrthoDB" id="1826at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="165" mass="19342" checksum="156E85D0BF026A39" modified="2018-07-18" version="1">MAIQIFDEENIRDMPSEERFNFCENVLKQEQDESKRWDAVWLAGELAENTNNNDRMRKKVADLMEWVLRNDTNGVVKHEASFQIAARNLRDKIPLLVEISFNDKSVLSKHEAIESLGLMRAFEVEEKIKLLRNDPSIDVRETAEFILKRFERLRNSGEYTPSTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSD3</accession>
    <name>A0A2S2KSD3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13810</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_264953952.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSD3"/>
    <dbReference type="GeneID" id="76209118"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="136" mass="15372" checksum="D9CF1190BA8445C5" modified="2018-07-18" version="1">MPINYTRVLSGNVILPVLFGIIVIGFAQLSFAEELESATFIAVNEEKFEQPQSRHSYQQVVIFGYIEDYSRGSQITFVIIAPDKSQIEITTYAARNGDIYTPIHITGDSQIGIHQLILKYQDEEIASTLFEILENQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSJ9</accession>
    <name>A0A2S2KSJ9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSJ9"/>
    <dbReference type="OrthoDB" id="10060at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="99" mass="11449" checksum="BADA88EE0A4F6D94" modified="2018-07-18" version="1">MPLTNNVIIKLNEITTMIEDKSKLSESDISVIKSIFQNLVENNERYDVDEIEFWFTNEGSWTKKESIIRIANLSSYVQDKYQQTAHLRIISDDDCGCGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSL9</accession>
    <name>A0A2S2KSL9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF47 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_14470</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0111 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877250.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSL9"/>
    <dbReference type="GeneID" id="56068026"/>
    <dbReference type="OrthoDB" id="9933at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002727">
        <property type="entry name" value="DUF47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018445">
        <property type="entry name" value="Put_Phosphate_transp_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36536">
        <property type="entry name" value="UPF0111 PROTEIN HI_1603"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36536:SF3">
        <property type="entry name" value="UPF0111 PROTEIN HI_1603"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01865">
        <property type="entry name" value="PhoU_div"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109755">
        <property type="entry name" value="PhoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="8"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="24491" checksum="0A68904CFFB77167" modified="2018-07-18" version="1">MYSGELEVQAKRKAIAVLQDEINRILNAARELATLPELMMKKDKTGIKNALEQISTIEEEVESLRRKITREVADVGGLIMNRENLLNTAYTMDEIAGYITGISFKLSNVKPATLKSSKLDQDITKLIELVVDEVYKLNEIIRSLNTNTANAIELAQETQTIEREIDIKYRDATIKLLNEVTSTKELMLIKDVIEGIEEMSDKCQRVSDSFILLALSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSM3</accession>
    <name>A0A2S2KSM3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_14150</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ABC-4 integral membrane protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877222.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSM3"/>
    <dbReference type="GeneID" id="76209080"/>
    <dbReference type="OrthoDB" id="11469at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050250">
        <property type="entry name" value="Macrolide_Exporter_MacB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30572:SF4">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE YTRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30572">
        <property type="entry name" value="MEMBRANE COMPONENT OF TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="277"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="322"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="367"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="MacB-like periplasmic core" evidence="9">
        <location>
            <begin position="21"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="ABC3 transporter permease C-terminal" evidence="8">
        <location>
            <begin position="282"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="410" mass="44141" checksum="9DB51F637BD5F473" modified="2018-07-18" version="1">MNPKEILLLSFSALNERKIRTLLTVLMVVVGSSLMIVLNGLSAGQTQFIEDQLNQLADNVLTVTPGQRSFRSVDTTPSIVFNSAVVNKINSLSSVVDVIPRYSGTVDLNSQARVLRTSVLAMNPDDVYVSVPGLEMEAGSVIKRNDPSAILVGQSVAYPDGASFPIVTLGQSLKLTFRYVDTEGEQQEDSRTFVVSGILKESGDRTLDRSVIINENVGNTFLKKSGKFDSLLVSLQSSELISQTEEDLKELFGTSIGISSLQSRLQFRQQFTEGNNAFIQSIGVIALVVGAVGIITTLYTSVTERIKEIGTMKAIGAQNRSILLLFLMEALLIGLLGGTAGILVGMMSGHALSSILSPPGFGNTSNIVPIFHFVDTLYVWLLSVILSISAGILPAWKASTLSPLVALRRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSN0</accession>
    <name>A0A2S2KSN0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF922 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12560</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877096.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSN0"/>
    <dbReference type="GeneID" id="76209247"/>
    <dbReference type="OrthoDB" id="4918at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="21471" checksum="BBAC236ADF3AF47D" modified="2018-07-18" version="1">MNENDVVVWSKETFLKWADFQAESNPATFEDSHSVIKYKFTWTINSEKIDEQILFLIENIRIYVEFFPHLSWVRISQDTDRLLNHEQGHFDLAEMIRQEYIEKLQAVFYGKHFPTRGQNEEQQKQFAKEDSGRMIAEEIEKLEQVLVKRRQEYDAMTEFGHNLEKQLEFDLKFNKLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSN8</accession>
    <name>A0A2S2KSN8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sugar transporter SemiSWEET</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_13520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877169.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSN8"/>
    <dbReference type="GeneID" id="76209147"/>
    <dbReference type="OrthoDB" id="11322at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004316">
        <property type="entry name" value="SWEET_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03083">
        <property type="entry name" value="MtN3_slv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="96" mass="10743" checksum="5EDB661325B12868" modified="2018-07-18" version="1">MEIDGMLLTILGISAGILILTGWIEQIYKGYKTKSLKDISKFLMIFISAGAILWLIYGVIVSDVFIIGTNITAIVLMMIVLAMKRNYDRLSKTNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSP2</accession>
    <name>A0A2S2KSP2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_14600</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877261.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSP2"/>
    <dbReference type="GeneID" id="76209035"/>
    <dbReference type="OrthoDB" id="2830at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050595">
        <property type="entry name" value="Bact_response_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591:SF3">
        <property type="entry name" value="RESPONSE REGULATORY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591">
        <property type="entry name" value="STRESS RESPONSE REGULATOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="3"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="129" mass="14201" checksum="12CB8780EE75FB02" modified="2018-07-18" version="1">MANILIADDSNAIRLVLKDILSIGEHDVIAEACDGSEAVEFYKKYNPDLMLLDLAMPKKDGYAVTKEIISYDPNAKIILITASDDQKTIQKCLNEGAFSYVSKPFDFNAVLDTIKEILKSNLGTIGTNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KST7</accession>
    <name>A0A2S2KST7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_15040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877298.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KST7"/>
    <dbReference type="GeneID" id="76208992"/>
    <dbReference type="OrthoDB" id="377703at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="171" mass="18999" checksum="151F4E26650450AE" modified="2018-07-18" version="1">MNHGYFEKSLKKVTNKIKFDGKNGFQKFFIHASFIVILIIATTTMAIADESILTQSFSNSEFIENSAVTPRGHIEDFSWTKRVSPDGVIEISGIGFSVINDDDIGHSFEICTIVQGPLEKFTPSLDSPLACTNTEKIEPYGKIENQFIDFSNGVKVSELVDISIAIQELDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSW0</accession>
    <name>A0A2S2KSW0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Small ribosomal subunit protein uS10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rps10</name>
        <name evidence="7" type="ORF">NZNM25_14420</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the binding of tRNA to the ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008299200.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSW0"/>
    <dbReference type="GeneID" id="40722503"/>
    <dbReference type="OrthoDB" id="371736at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.600:FF:000004">
        <property type="entry name" value="30S ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="entry name" value="Ribosomal protein S10 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_uS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_uS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_uS10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_uS10_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005729">
        <property type="entry name" value="Ribosomal_uS10_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01046">
        <property type="entry name" value="uS10_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00971">
        <property type="entry name" value="RIBOSOMALS10"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="Ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS10" evidence="6">
        <location>
            <begin position="6"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="102" mass="11392" checksum="BA2FAA4B664E5BEF" modified="2018-07-18" version="1">MTQTARVKLTSTSLPKLDGVCGEIMGIGKKTGVKVKGPTPLPVKRLHVATRKSPCGSGTETYEKWEMKMHRRIININADDKAIRQLMRLKIPDDVYIELSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSZ0</accession>
    <name>A0A2S2KSZ0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphoribosyl-AMP cyclohydrolase</fullName>
            <shortName evidence="7">PRA-CH</shortName>
            <ecNumber evidence="7">3.5.4.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hisI</name>
        <name evidence="9" type="ORF">NZNM25_15450</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the hydrolysis of the adenine ring of phosphoribosyl-AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>1-(5-phospho-beta-D-ribosyl)-5'-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:20049"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="EC" id="3.5.4.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PRA-CH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.19" evidence="7"/>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877378.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSZ0"/>
    <dbReference type="GeneID" id="76208443"/>
    <dbReference type="OrthoDB" id="5853at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00008"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004635">
        <property type="term" value="F:phosphoribosyl-AMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004636">
        <property type="term" value="F:phosphoribosyl-ATP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.810:FF:000001">
        <property type="entry name" value="Histidine biosynthesis bifunctional protein HisIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.810">
        <property type="entry name" value="Phosphoribosyl-AMP cyclohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01021">
        <property type="entry name" value="HisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026660">
        <property type="entry name" value="PRA-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002496">
        <property type="entry name" value="PRib_AMP_CycHydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038019">
        <property type="entry name" value="PRib_AMP_CycHydrolase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000768">
        <property type="entry name" value="PRK00051.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42945">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS BIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42945:SF1">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS BIFUNCTIONAL PROTEIN HIS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01502">
        <property type="entry name" value="PRA-CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141734">
        <property type="entry name" value="HisI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phosphoribosyl-AMP cyclohydrolase" evidence="8">
        <location>
            <begin position="32"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="109" mass="12270" checksum="F670FBC6BB76251E" modified="2018-07-18" version="1">MQKSIDDIDFEKSGGLVPVIVQDILTKDVLTLAYANKESLELAKKTGNSWFWSRSRNKLWMKGEESGNTQKIKEILVDCDSDAIIYLVEPLGPACHLGEKVCFHNSLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT02</accession>
    <name>A0A2S2KT02_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TATA-box-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Box A-binding protein</fullName>
            <shortName evidence="7">BAP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">TATA sequence-binding protein</fullName>
            <shortName evidence="7">TBP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">TATA-box factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tbp</name>
        <name evidence="9" type="ORF">NZNM25_13760</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7 8">General factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Binds specifically to the TATA box promoter element which lies close to the position of transcription initiation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the TBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877190.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT02"/>
    <dbReference type="GeneID" id="76209123"/>
    <dbReference type="OrthoDB" id="350539at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000007">
        <property type="entry name" value="TATA-box-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000010">
        <property type="entry name" value="TATA-box-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00408">
        <property type="entry name" value="TATA_bind_prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030491">
        <property type="entry name" value="TBP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00686">
        <property type="entry name" value="TIFACTORIID"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00351">
        <property type="entry name" value="TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="repeat" description="1" evidence="7">
        <location>
            <begin position="10"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="repeat" description="2" evidence="7">
        <location>
            <begin position="101"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="186" mass="20674" checksum="A493C5D4A33AC08E" modified="2018-07-18" version="1">MTETKPVIAIVNVVASATIEQKLDLVDITKKFPDVEYHPEQFPGAVFRLKTPRTATLLFGSGKMVCTGAKSEEMAIKAVNIVVQNLRKGKIKIKNEPIVTIQNIVSSINLGGKVNLEQAARTLPRSMYEPEQFPGLIHRMLDPKTVILIFASGKLVCVGARIEKDIHRSVNQIHSLLEEKNLMVYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT04</accession>
    <name>A0A2S2KT04_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Thrombospondin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_15600</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT04"/>
    <dbReference type="OrthoDB" id="3044at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1080.10">
        <property type="entry name" value="TSP type-3 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003367">
        <property type="entry name" value="Thrombospondin_3-like_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028974">
        <property type="entry name" value="TSP_type-3_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10199">
        <property type="entry name" value="THROMBOSPONDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02412">
        <property type="entry name" value="TSP_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103647">
        <property type="entry name" value="TSP type-3 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="489" mass="54772" checksum="A91A7D91A93C2405" modified="2018-07-18" version="1">MVYFVVDKKIKFLLVLLGITFGVSFLLSEFATFADSDKDSIMDSIDNCPLNVNSDQADFDFDGVGDECDTNDDNDMVSDYLDQFDTDPLDWSDFDFDGVGSNKDTDDDNDGILDVDDSTPLLSSEILTIKYLQDIDTCAYMGDSTSRLVCYSQFFGKLVKSEKNNLDALELSIALSKIGTVDDCHFISHEIGHVAYDETRDVTKSLQGMDGTMCRGGYFHGVLASYFHNIGKSEASFPNSYQTICDDLIGSSNYQDCIHGLGHGFVHYFGDDLNSSLESCDDLSFYQDILCVKGVMMQYTDNAFTRDGISKNVISNLCNAKQLEKNDYVECSMSTGTTLAFFTNHDFEKGKELCNLIEEPDTRNYCIEGLRLEIQDSEKYEDDPLTKENREKFQPQFIKGTKTIDIRSPAVVSNFEFIPEIGMISFSIDKPQYVILYIPKEFVASKMLVTVNGQIPGQLESQNNVLDKDIAMIKFVPDEPGLVLISPFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT23</accession>
    <name>A0A2S2KT23_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protoheme IX farnesyltransferase</fullName>
            <ecNumber evidence="11">2.5.1.141</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Heme B farnesyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Heme O synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">ctaB</name>
        <name evidence="12" type="ORF">NZNM25_15840</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>heme b + (2E,6E)-farnesyl diphosphate + H2O = Fe(II)-heme o + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:28070"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.141"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Porphyrin-containing compound metabolism; heme O biosynthesis; heme O from protoheme: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cell membrane</location>
            <topology evidence="2 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="11">Carbon 2 of the heme B porphyrin ring is defined according to the Fischer nomenclature.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the UbiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the UbiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.141" evidence="11"/>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877411.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT23"/>
    <dbReference type="GeneID" id="76208404"/>
    <dbReference type="OrthoDB" id="131615at2157"/>
    <dbReference type="UniPathway" id="UPA00834">
        <property type="reaction ID" value="UER00712"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008495">
        <property type="term" value="F:protoheme IX farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048034">
        <property type="term" value="P:heme O biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13957">
        <property type="entry name" value="PT_UbiA_Cox10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.140:FF:000019">
        <property type="entry name" value="Protoheme IX farnesyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00154">
        <property type="entry name" value="CyoE_CtaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006369">
        <property type="entry name" value="Protohaem_IX_farnesylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01473">
        <property type="entry name" value="cyoE_ctaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448:SF2">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 11" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="178"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH34793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="309" mass="33856" checksum="2F0C94AD7A3D4018" modified="2018-07-18" version="1">MQKQKSESRVAVYYELTKPKIWYLLVFTAFGAALTASNIYDIEISPATWLLMLFSVAAGSAAANTLTNYHDRDIDAIMERTKGRPLPSKRIYPAEKARNFGLVLAGISLVLAFGISFTTTLEQGAWATAFIAFGLVNNILVYSYALKRNSRTNIILGGLCGGSPPMIGWVAVSMSDLWTMGLAMAGLVFIWIPMHIWALTLHFKDDYNKVNVPMLTAVQSEKTSARAIAGSTVIMVLFSIAPFFITTESGDSMVGSVYLWTAIVSGALMIALSIWVIVKPMEKASWTLFKFSSPYLAVLFIALMVDSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT26</accession>
    <name>A0A2S2KT26_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase</fullName>
            <ecNumber evidence="9">4.1.3.44</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">tRNA wyosine derivatives biosynthesis protein Taw1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">taw1</name>
        <name evidence="11" type="ORF">NZNM25_15220</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the wyosine derivatives biosynthesis pathway that catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine (imG-14) on guanosine-37 of tRNA(Phe).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine + L-methionine + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:36347"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10165"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="EC" id="4.1.3.44"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TYW1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.44" evidence="9"/>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877360.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT26"/>
    <dbReference type="GeneID" id="76208466"/>
    <dbReference type="OrthoDB" id="68499at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102521">
        <property type="term" value="F:tRNA-4-demethylwyosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01921">
        <property type="entry name" value="TYW1_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013917">
        <property type="entry name" value="tRNA_wybutosine-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034556">
        <property type="entry name" value="tRNA_wybutosine-synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023993">
        <property type="entry name" value="TYW1_archaea"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03972">
        <property type="entry name" value="rSAM_TYW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930:SF0">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE TYW1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08608">
        <property type="entry name" value="Wyosine_form"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00284">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01071">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Radical SAM core" evidence="10">
        <location>
            <begin position="60"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="342" mass="40157" checksum="1DF777153E2C1427" modified="2018-07-18" version="1">MSCSGEIIEEDDRLIQIKPAISEQLKKAKYGVADHSTVELCHWTKKSFKHEGSCYKHKFYGISTHRCMEFSPAGMHCENRCVYCWRPMEFYDSMKMEPEQVAEPKEILTKLMAERKKLINGYYGDSRNDKQRLDESLLPSHYAISLSGEPTMYPKLPELIKYLNSLESTKSIFLVTNGQEPDMIQKLQDEDALPTQLYLSTNAADYESFIRINKPKYDDSWERWNRTLNMLKDLNTRTVLRVTLIRNYNDQKESIPAFAEMFRKASPHFIEIKSYMHIGRSTNRLEHANMLEMEEVKKFSEEIAKQSRVFSVMDESIISRISILQNNERYIDRFIPTYSNTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT72</accession>
    <name>A0A2S2KT72_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_14490</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877252.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT72"/>
    <dbReference type="GeneID" id="76209046"/>
    <dbReference type="OrthoDB" id="7569at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="125" mass="13914" checksum="96E7EF817414188B" modified="2018-07-18" version="1">MQDPDPLPWGALDRFQAHFIVKRDSGTSVGNFVAKTKLTTKGHFASKTVEKVEWDGPGSLASKLNADAELNEMIAKQSVKDATIYVEPTDGAIRIRNKWNNHLSFGITKDLFEIYDRIAGHIKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTB3</accession>
    <name>A0A2S2KTB3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Blue (type 1) copper domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_16740</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877499.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTB3"/>
    <dbReference type="GeneID" id="76208311"/>
    <dbReference type="OrthoDB" id="12245at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000923">
        <property type="entry name" value="BlueCu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052721">
        <property type="entry name" value="ET_Amicyanin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507:SF1">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00127">
        <property type="entry name" value="Copper-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Blue (type 1) copper" evidence="4">
        <location>
            <begin position="196"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="275" mass="31421" checksum="CB826D65DA2D557F" modified="2018-07-18" version="1">METIPKVGILVGIVAVVIFLPFWMIVTIAENEALFVEFFFNVDLVEKKMKETKTYKAMLERYPDSIVSLRNHGPFNSDMEIIAYSNSSESHLNANIGFDHQNDFTWEHVRCQIGDGDDRKKLGTIPPELIENNPPIPRYMFKEGNADNSFTADFIKFTNCLELNKEQIELQELDADYHVAIPENTGVPGCQEDLSCFEPYSLKIKVGDIVAFRNFDSDYHTVTSGSPEFGPGGEFDSALMESGEQFFHKFTKADEYEYFCMVHPWQTGKIIVHEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTD3</accession>
    <name>A0A2S2KTD3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Peptidase M24</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_16940</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M24B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877514.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTD3"/>
    <dbReference type="GeneID" id="76208292"/>
    <dbReference type="OrthoDB" id="1346at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050659">
        <property type="entry name" value="Peptidase_M24B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46112">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46112:SF9">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase N-terminal" evidence="5">
        <location>
            <begin position="4"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24" evidence="4">
        <location>
            <begin position="132"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="354" mass="40101" checksum="57817B47EE4CD554" modified="2018-07-18" version="1">MKQRRKNLLKFAQKIDCDTLVTFEPENLFYMTGFWGEAIGLLEKNGKTTIIAPELEVGRAQEESEDCDVITAERGTGLISSLIQKIKKNQVCTDCQNYSVMMSLKKSIPKITSSTEPFYNSRIIKDEKEIQILKKASKIIDEMFNLCSKKMKIGQKESELQTILMTYAMEQEMFDTGYKSTLNPLIIAGGPNGALPHAQVTQRKFKNGDLIVTDLTLRYKGYVSDATRTFAIGKISQQANEAYEIVKESQKLGIKAVKPNAYCKDVDFACRQYIEEKNYGQYFIHSTGHGIGLEVHELPTISYKSETKLQKNMAITVEPGIYIANKFGIRIEDSLIVNEKPIVMHKFTKDLVQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTK7</accession>
    <name>A0A2S2KTK7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CCA-adding enzyme</fullName>
            <ecNumber evidence="10">2.7.7.72</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">CCA tRNA nucleotidyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA CCA-pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA adenylyl-/cytidylyl- transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA nucleotidyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA-NT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cca</name>
        <name evidence="14" type="ORF">NZNM25_17570</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair. Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs. While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14433"/>
            <dbReference type="Rhea" id="RHEA-COMP:10465"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:74896"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="EC" id="2.7.7.72"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a tRNA with a 3' CCA end + 2 CTP + ATP = a tRNA with a 3' CCACCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:76235"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="Rhea" id="RHEA-COMP:18655"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="ChEBI" id="CHEBI:195187"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="10">A single active site specifically recognizes both ATP and CTP and is responsible for their addition.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.72" evidence="10"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877564.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTK7"/>
    <dbReference type="GeneID" id="76208228"/>
    <dbReference type="OrthoDB" id="7378at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:CCA tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160016">
        <property type="term" value="F:CCACCA tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042245">
        <property type="term" value="P:RNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001680">
        <property type="term" value="P:tRNA 3'-terminal CCA addition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05400">
        <property type="entry name" value="NT_2-5OAS_ClassI-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1550">
        <property type="entry name" value="Archaeal tRNA CCA-adding enzyme catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.30">
        <property type="entry name" value="CCA tRNA nucleotidyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.590">
        <property type="entry name" value="Poly(A) polymerase predicted RNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01264">
        <property type="entry name" value="CCA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048833">
        <property type="entry name" value="CAA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008229">
        <property type="entry name" value="CCA-adding_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042090">
        <property type="entry name" value="CCA_tRNA_nucleotrans_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006116">
        <property type="entry name" value="NT_2-5OAS_ClassI-CCAase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011068">
        <property type="entry name" value="NuclTrfase_I-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015329">
        <property type="entry name" value="tRNA_NucTransf2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03671">
        <property type="entry name" value="cca_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39643">
        <property type="entry name" value="CCA-ADDING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39643:SF1">
        <property type="entry name" value="CCA-ADDING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21133">
        <property type="entry name" value="CAA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09249">
        <property type="entry name" value="tRNA_NucTransf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005335">
        <property type="entry name" value="CCA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55003">
        <property type="entry name" value="PAP/Archaeal CCA-adding enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0692">RNA repair</keyword>
    <keyword evidence="9 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="11">
        <location>
            <begin position="40"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA nucleotidyltransferase substrate binding" evidence="12">
        <location>
            <begin position="152"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="CCA-adding enzyme C-terminal" evidence="13">
        <location>
            <begin position="273"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF09249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF21133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GBH34966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="443" mass="50696" checksum="B2962AC984B74260" modified="2018-07-18" version="1">MNQVINKVTKDVIPSKVIEQSKKKIADLAFKLVEREIGKFPEVIGLEFGGSYAKGTWLSKDADIDIFVRFKKSTSEEKFEKISKKIGFASLKEYSPYVRYSEHPYVEAKINNTKINVVPFYDVKIGEWKSSADRSTFHTKFMQKSLTLKMKNEVRVLKTFLKSNNIYGAEIAKQGLSGYVSEVLILHFGSFENVIKSISQIKENQVIGKTSKNFETPIVVIDPIDSNRNLAAAISNENIGKFILICRAFKDKATLTFFKNKKLRTSKKYWENLLVIKFNFKIRSPDIIWGQIKRATSSLSTQLELAGFNVLRSKSYTDQQKEVYLFFFLESSKIVQIYSKNGPDFFREDSSKSFISKNLKNSELMWVGNNGKIISLEKRKHVDAIKFMSEFLKKNLQTCIPKGLQSDFKRGYKISVGKKNLSKSIKEAASELISTDGTLIYFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTL5</accession>
    <name>A0A2S2KTL5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_17600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTL5"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="62" mass="7202" checksum="7BC3E91105CD9DBF" modified="2018-07-18" version="1">MVVLQVLGQFYKIKTILLTILVSITKHYEFFYLLYVETISAMNPIPLTIAVPYKAPFFLSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTM0</accession>
    <name>A0A2S2KTM0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Proteasome subunit beta</fullName>
            <ecNumber evidence="9">3.4.25.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">20S proteasome beta subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Proteasome core protein PsmB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">psmB</name>
        <name evidence="11" type="ORF">NZNM25_16980</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.25.1" evidence="9"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877517.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTM0"/>
    <dbReference type="GeneID" id="76208288"/>
    <dbReference type="OrthoDB" id="6330at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000049">
        <property type="entry name" value="Proteasome subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02113_A">
        <property type="entry name" value="Proteasome_B_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019983">
        <property type="entry name" value="Pept_T1A_Psome_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03634">
        <property type="entry name" value="arc_protsome_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF0">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE SUBUNIT HSLV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0645">Protease</keyword>
    <keyword evidence="7 9 11" id="KW-0647">Proteasome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="8 9" id="KW-0865">Zymogen</keyword>
    <feature type="propeptide" id="PRO_5015790865" description="Removed in mature form; by autocatalysis" evidence="9">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023334034" description="Proteasome subunit beta" evidence="9">
        <location>
            <begin position="13"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9 10">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="210" mass="22806" checksum="19D330A487DEDE06" modified="2018-07-18" version="1">MSNNVEEKILHGTTTVGIKAKDGVVLCADMRASAGYFIANNNTMKIQKIDLHAGLTLAGGVADAQNIVDILRYHANLHRVEKQGPISIHSLSRLCSLIFHQNRGYPFMADILVGGYDANGPALFNIDMFGSVEKKSYVTTGSGSPVAYGLLEEEYREDLTVEEAKKIALRAVKAAIVRNIGTGDGINIAIMDKDGFRLLTDEQKKAVIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTP6</accession>
    <name>A0A2S2KTP6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_18150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877616.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTP6"/>
    <dbReference type="GeneID" id="76210306"/>
    <dbReference type="OrthoDB" id="6896at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="83" mass="9670" checksum="A6D88F6CC5ED6CF6" modified="2018-07-18" version="1">MGANPYVHIPKESWPSWTWYAIEFGIVIAISMLVSREITNSFENLTPELQNYVFMGIVGLIFLAWYIGIRGFILKKKILQNKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTS8</accession>
    <name>A0A2S2KTS8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">A-type ATP synthase subunit A</fullName>
            <ecNumber evidence="10">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">atpA</name>
        <name evidence="12" type="ORF">NZNM25_16590</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane. The A chain is the catalytic subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="10"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877484.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTS8"/>
    <dbReference type="GeneID" id="76208326"/>
    <dbReference type="OrthoDB" id="115235at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18111">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18119">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01134">
        <property type="entry name" value="V_A-ATPase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00309">
        <property type="entry name" value="ATP_synth_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031686">
        <property type="entry name" value="ATP-synth_a_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022878">
        <property type="entry name" value="V-ATPase_asu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003220">
        <property type="entry name" value="PRK04192.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607:SF1">
        <property type="entry name" value="H(+)-TRANSPORTING TWO-SECTOR ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607">
        <property type="entry name" value="V-TYPE PROTON ATPASE CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16886">
        <property type="entry name" value="ATP-synt_ab_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="226"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="234"/>
            <end position="241"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH34868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="592" mass="65389" checksum="BA423C2D9A43B344" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTU0</accession>
    <name>A0A2S2KTU0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Creatinine amidohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_18530</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877677.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTU0"/>
    <dbReference type="GeneID" id="76210079"/>
    <dbReference type="OrthoDB" id="46121at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10310">
        <property type="entry name" value="Creatininase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024087">
        <property type="entry name" value="Creatininase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003785">
        <property type="entry name" value="Creatininase/forma_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35005:SF1">
        <property type="entry name" value="2-AMINO-5-FORMYLAMINO-6-RIBOSYLAMINOPYRIMIDIN-4(3H)-ONE 5'-MONOPHOSPHATE DEFORMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35005">
        <property type="entry name" value="3-DEHYDRO-SCYLLO-INOSOSE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02633">
        <property type="entry name" value="Creatininase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102215">
        <property type="entry name" value="Creatininase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH35062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="240" mass="27104" checksum="EEBEA64D6D0EFEA4" modified="2018-07-18" version="1">MTDIKSQLDPDLRKLISKNKQVAIIPVGSIEQHGSHLPISTDTDIVTEVAKRISEKNRYLLLPTITFGVSFEHAPFFNLSIRESTLRTVLSDLCISLLSNRIKTVFIINGHHGNLKPIKNIDIRLKKLSKNKLKVFPLSYWHFMNREFDHAGFVETSLMLAISKNVKMKLAKKGLTTDKMTKQQIQKLGKLANKSFPKATKNGIWGDPTKATKKDGQRILDEIINNLSKRCQTCLTGHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTV3</accession>
    <name>A0A2S2KTV3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Large ribosomal subunit protein eL8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rpl7ae</name>
        <name evidence="11" type="ORF">NZNM25_18500</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, the RNA component of RNase P, box H/ACA, box C/D and box C'/D' sRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Part of the 50S ribosomal subunit. Probably part of the RNase P complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877675.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTV3"/>
    <dbReference type="GeneID" id="56066683"/>
    <dbReference type="OrthoDB" id="25810at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000020">
        <property type="entry name" value="50S ribosomal protein L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00326">
        <property type="entry name" value="Ribosomal_eL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022481">
        <property type="entry name" value="Ribosomal_eL8_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03677">
        <property type="entry name" value="eL8_ribo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00884">
        <property type="entry name" value="RIBOSOMALHS6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 9 11" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 9" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="10">
        <location>
            <begin position="19"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH35059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="128" mass="13476" checksum="5A0D16825F68F63F" modified="2018-07-18" version="1">MGKSYYVKFETPEDLVNPILEAVRVASTSGKVKKGTNEATKAIERGTSKLIVIAEDVEPPEVVAHLPILCEEQGAAYAFVPSKQELGKSLGIDITSAAAAILDAGDAQHIVDQVVSSIAKIKGGKTDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTW1</accession>
    <name>A0A2S2KTW1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PRC-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_18730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877697.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTW1"/>
    <dbReference type="GeneID" id="76210100"/>
    <dbReference type="OrthoDB" id="9907at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.240">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC-barrel" evidence="1">
        <location>
            <begin position="150"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="241" mass="26283" checksum="32DA0746EE5995E8" modified="2018-07-18" version="1">MSSEQRLKKLRGSGGYVMATVTDEQQMKGNLGGPDLFLAPIGRLEAERITKHFCNTCEKEFEGSPKIEFENPNEEVAENLVLYEKGQYICNACNASIAEYREFRKQDESGEVGVAKPIEPSTQNIPQQVAEPTIQTPQETVTQPGPTTSVSSIEGRVVYDENANKIGNAKQVGIDSTQSMVLVITKNDGTEGSIPWSNIKKVGEIILLGNPEEKSQPGKCSNCGFGNKEGSKFCEECGTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTX3</accession>
    <name>A0A2S2KTX3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_18670</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877692.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTX3"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="101" mass="12237" checksum="8E5E0F774777E465" modified="2018-07-18" version="1">MGGMWYHLDRQKRNVQCPVCAWIFPCRRSDGQCSKCGHRFNTDYNVYKSEETRRKLRKEHYKKLEQLEQILQNCIYEIRTIKHELTPLDLPLNIQNEQLPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTZ1</accession>
    <name>A0A2S2KTZ1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SNF7 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_18420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877641.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTZ1"/>
    <dbReference type="GeneID" id="76210279"/>
    <dbReference type="OrthoDB" id="6919at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="202"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="23650" checksum="28B6D7974EF0DAE4" modified="2018-07-18" version="1">MPNFDKTWAHQETQSVTGKLREAVKPQGALKPRIQTAVNKLQVQISKMDSMLGKLHERDAQLFQRVVTAMQQHDTSTSRVLSNELAEIRKVTKMLGNARMSLEQVQLRLTTIHDLGDAMVAIGPAMSTMKGLKSSLGRFMPEADSELNSMTQTLNGLMMDSLAGDSFSMESAASNEETEKILQEASAVAEQQIGDRFPSVPTSTGLSSQSSTTTFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU00</accession>
    <name>A0A2S2KU00_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Formyltetrahydrofolate deformylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_18520</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU00"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008864">
        <property type="term" value="F:formyltetrahydrofolate deformylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.170">
        <property type="entry name" value="Formyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004810">
        <property type="entry name" value="PurU"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004684">
        <property type="entry name" value="PRK06027.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42706">
        <property type="entry name" value="FORMYLTETRAHYDROFOLATE DEFORMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42706:SF1">
        <property type="entry name" value="FORMYLTETRAHYDROFOLATE DEFORMYLASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13740">
        <property type="entry name" value="ACT_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036480">
        <property type="entry name" value="FormyFH4_hydr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01575">
        <property type="entry name" value="FFH4HYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="3">
        <location>
            <begin position="16"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH35061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="289" mass="32695" checksum="3A465CFB4035C77D" modified="2018-07-18" version="1">MRKSSPNKVMKKTVVGITIVGKDREGIVASFTNFAFSKGGNIEKVNQNVIKGLFGMYLEVSFSKSLDVKKFDSEIQNLAKKEKMDVSTHHETNSEKNIAVFVTKEPLCLETILKNTKSLKGKISVIIGTENTLESLAKKAKIPFVAVEENNQEKAEEKIIQICKKYDVDLIALARYMRILSPNFVWRYPNRIINIHPSLLPAFPGALAYAQAFERGTKIVGVTSHYVTENLDQGPIIFQDSFKVDPNDTLEQIKTKGQKLEADTLLKAMKMHLENKLEVRWRKVHIKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU15</accession>
    <name>A0A2S2KU15_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Iron permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_19070</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxidase-dependent Fe transporter (OFeT) (TC 9.A.10.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU15"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033573">
        <property type="term" value="C:high-affinity iron permease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004923">
        <property type="entry name" value="FTR1/Fip1/EfeU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31632">
        <property type="entry name" value="IRON TRANSPORTER FTH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31632:SF2">
        <property type="entry name" value="PLASMA MEMBRANE IRON PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03239">
        <property type="entry name" value="FTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="448"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="479"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="513"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="547"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="588"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="621"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="680"/>
            <end position="700"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH35116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="720" mass="81157" checksum="F466D22BE44196B5" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU34</accession>
    <name>A0A2S2KU34_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Potassium transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_17590</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877566.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU34"/>
    <dbReference type="GeneID" id="76208226"/>
    <dbReference type="OrthoDB" id="43518at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42751">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER FAMILY/TRKA DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42751:SF3">
        <property type="entry name" value="SODIUM_GLUTAMATE SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="227"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="274"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="298"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="326"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="358"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="428"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="470"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="518"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="550"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="8">
        <location>
            <begin position="22"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="593" mass="64473" checksum="4FB1722CCB143017" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU37</accession>
    <name>A0A2S2KU37_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dTDP-4-dehydrorhamnose 3,5-epimerase</fullName>
            <ecNumber evidence="2">5.1.3.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Thymidine diphospho-4-keto-rhamnose 3,5-epimerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_19550</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the epimerization of the C3' and C5'positions of dTDP-6-deoxy-D-xylo-4-hexulose, forming dTDP-6-deoxy-L-lyxo-4-hexulose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-L-rhamnose</text>
            <dbReference type="Rhea" id="RHEA:16969"/>
            <dbReference type="ChEBI" id="CHEBI:57649"/>
            <dbReference type="ChEBI" id="CHEBI:62830"/>
            <dbReference type="EC" id="5.1.3.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dTDP-4-dehydrorhamnose 3,5-epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.13" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877765.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU37"/>
    <dbReference type="GeneID" id="76210185"/>
    <dbReference type="OrthoDB" id="2990at2157"/>
    <dbReference type="UniPathway" id="UPA00124"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008830">
        <property type="term" value="F:dTDP-4-dehydrorhamnose 3,5-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019305">
        <property type="term" value="P:dTDP-rhamnose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00438">
        <property type="entry name" value="cupin_RmlC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000888">
        <property type="entry name" value="RmlC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01221">
        <property type="entry name" value="rmlC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21047">
        <property type="entry name" value="DTDP-6-DEOXY-D-GLUCOSE-3,5 EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21047:SF2">
        <property type="entry name" value="THYMIDINE DIPHOSPHO-4-KETO-RHAMNOSE 3,5-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00908">
        <property type="entry name" value="dTDP_sugar_isom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR600888-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU364069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="183" mass="21127" checksum="A3C7B727856FA8D6" modified="2018-07-18" version="1">MGFTFKRLSIPEVILVEPKLYSDSRGFFYESFKESDFFSNEITKFVQDNVSHSINGVIRGLHYQINPKPQAKLVSVFNGKIFDVAVDIRKKSPTYGRWVAEILSDENHRSLYVPEGFAHGFCVMSKEADVFYKVSNEHSPENERGIIWNDPTLNIPWSVKKPIISDKDSKLPLLEETENNFIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU53</accession>
    <name>A0A2S2KU53_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NAD-dependent dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_19630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_160049230.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU53"/>
    <dbReference type="GeneID" id="76210193"/>
    <dbReference type="OrthoDB" id="358920at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01713">
        <property type="entry name" value="NUCEPIMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="1">
        <location>
            <begin position="5"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="294" mass="33579" checksum="5EC698A34E8C8B3E" modified="2018-07-18" version="1">MDEILVTGITGFIGNHLVPELSKNYKILGISKNKKKSTKNFKLRTMDITKSDFISRIPFSKIVHMAAYSDVNYCNLNPTKCFELNVKSTQKMLEVARKNDSSFIFLSSSHVYGTPQKLPLSEKDPCYPLTHYAASKKMSEILCETYSQTYDLDIRIARIFSVYGPKSPKSNLVFNIMNQMINNSKIKLGNTSPKRDFIFISDVINGLIKIINSKRKGFQTYNIGSGNSTSVKEIVKTCLKIHGKNLELISSKEKIRKNEISDIYANITKMKSEFNWKPQVSLKRGLEITYNYYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU79</accession>
    <name>A0A2S2KU79_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Radical SAM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_19800</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829119.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU79"/>
    <dbReference type="GeneID" id="76210210"/>
    <dbReference type="OrthoDB" id="49555at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034471">
        <property type="entry name" value="GDGT/MA_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034474">
        <property type="entry name" value="Methyltransferase_Class_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056488">
        <property type="entry name" value="Zn_ribbon_HMPTM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045702">
        <property type="entry name" value="rSAM_GDGT_ether"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43306">
        <property type="entry name" value="7,8-DIHYDRO-6-HYDROXYMETHYLPTERIN DIMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43306:SF1">
        <property type="entry name" value="7,8-DIHYDRO-6-HYDROXYMETHYLPTERIN DIMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23545">
        <property type="entry name" value="Zn_ribbon_HMPTM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00385">
        <property type="entry name" value="7_8-dihydro-6-hydroxymethylpte"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01100">
        <property type="entry name" value="methyltransferase_(Class_D)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="104"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH35189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="554" mass="63178" checksum="798560E5FD27E747" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUD3</accession>
    <name>A0A2S2KUD3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dolichyl-phosphate mannose synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_19580</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877768.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUD3"/>
    <dbReference type="GeneID" id="76210188"/>
    <dbReference type="OrthoDB" id="11098at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04179">
        <property type="entry name" value="DPM_DPG-synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050256">
        <property type="entry name" value="Glycosyltransferase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48090:SF7">
        <property type="entry name" value="RFBJ PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48090">
        <property type="entry name" value="UNDECAPRENYL-PHOSPHATE 4-DEOXY-4-FORMAMIDO-L-ARABINOSE TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="260"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="2">
        <location>
            <begin position="5"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="294" mass="32236" checksum="5DDBFDCA48C71E2C" modified="2018-07-18" version="1">MKVIIGIPAFNEERNIGSIIAKLKQKYAQIIVCDDGSSDMTSVIASLMGVHVVKHDKNMGYGSAIKTIFNESKKIEGDVLVTFDADGQHQISEIDSMLKPIYDNKADIVIGSRFLGTTKGLPTYRKIGIKTITGLTNVMTGSKITDSQSGFRAYKKNVIDQIFPTESGMGISTEILIKASKKNMRITEIPITISYENNTHSQEPISHGTSVVMSTVKHVAIERPLLYYGVTGLCFLILGLIFGAWTMQIYSEERVVMTNIALIGIGGVILGTILLITATILYSIVNVVRENRQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUK3</accession>
    <name>A0A2S2KUK3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_20280</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877848.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUK3"/>
    <dbReference type="GeneID" id="76210263"/>
    <dbReference type="OrthoDB" id="8749at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005372">
        <property type="entry name" value="UPF0182"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39344">
        <property type="entry name" value="UPF0182 PROTEIN SLL1060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39344:SF1">
        <property type="entry name" value="UPF0182 PROTEIN SLL1060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03699">
        <property type="entry name" value="UPF0182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="120"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="183"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH35237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="952" mass="107234" checksum="586F688FCEA2A58F" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNN0</accession>
    <name>A0A2S2KNN0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Small ribosomal subunit protein uS19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rps19p</name>
        <name evidence="8" type="ORF">NZNM25_00240</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875906.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNN0"/>
    <dbReference type="GeneID" id="76209350"/>
    <dbReference type="OrthoDB" id="30559at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.860.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="entry name" value="30s Ribosomal Protein S19, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_uS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_uS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_uS19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005713">
        <property type="entry name" value="Ribosomal_uS19_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_uS19_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003121">
        <property type="entry name" value="PRK04038.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01025">
        <property type="entry name" value="uS19_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="RIBOSOMAL PROTEIN S19P FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="Ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="131" mass="14704" checksum="B38D7B87EA44C676" modified="2018-07-18" version="1">MVKEFLYRGIPKEDLDSMSLEKLFLLFNSRQRRSLTRGITDGKRKLIEEIKAAKAGKVKNPIKTHVRDLIVLPYMVGVTVNVFSGKEFRPVSITAEMIGHYLGEYVITNKRVSHGAPGVGASRSSLYVPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNR9</accession>
    <name>A0A2S2KNR9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="7">ATase</shortName>
            <ecNumber evidence="7">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
            <shortName evidence="7">GPATase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 13" type="primary">purF</name>
        <name evidence="13" type="ORF">NZNM25_00500</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + L-glutamine + H2O</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7 10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="7 11">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7 8">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="7"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875930.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNR9"/>
    <dbReference type="GeneID" id="76209379"/>
    <dbReference type="OrthoDB" id="5976at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01931">
        <property type="entry name" value="PurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11907">
        <property type="entry name" value="AMIDOPHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3 7 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 11" id="KW-0408">Iron</keyword>
    <keyword evidence="7 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="12">
        <location>
            <begin position="6"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7 9">
        <location>
            <position position="6"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="451"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="454"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH33259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="477" mass="52189" checksum="F6B8B16A6F9C0CDA" modified="2018-07-18" version="1">MVKENCGVVGIFSLSGTNVVPMVIDALRALQHRGQEAWGLAIPNKPPLKRLGLVSGSSSEFKKITEEYSSPAVIGHVRYSTMGRSSLENAQPLKVKDLCIAHNGTIANVQELSNLVGGCSFTPQNASDTLVAAQRLVTLISENGQMGKALSILKNEMVGSYCFTFISDDNSVYAARDPKGFRPMVLGYKESDDTYIVASESSAVSAVGAKLQRNVNPGELIKLSKNGLETERFSDDLTRAHCSFEFTYFAHPSSNMEGTNIYVARKNIGRFLAKKFPINDADLVIPVPDSARPAALGYAQELGVSFDEGLLKDRYSKKGPLRSFIEPHQSDRIEINRWIIPIKEIIEGKHVVVIDDSLVRGTSSKAIIKALRRAGARKISMVITYPPIKFPCYAGIDFPSREELATFSDGKEMSEIEITEMVRQSIGADFLGYNDAENLAAAVGIPKDSMCFTCSSGNYESLGITPEFRSREEMKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNT8</accession>
    <name>A0A2S2KNT8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_00180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875901.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNT8"/>
    <dbReference type="GeneID" id="76209344"/>
    <dbReference type="OrthoDB" id="4808at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="122" mass="14109" checksum="88CF900EFE799B48" modified="2018-07-18" version="1">MNPMFGFTIGGLMMIAVIMVIAFSIIGSFTSVSSEKSLVKEVNSALNMDEPKMCLKFDNPQECIGNFAYMKRSPQICLDYLEEENQYECLSKFFRKFQDRVCNYVSADFYDKCTIEAQKWKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNV3</accession>
    <name>A0A2S2KNV3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_00380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875921.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNV3"/>
    <dbReference type="GeneID" id="76209366"/>
    <dbReference type="OrthoDB" id="10600at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="75" mass="8248" checksum="728AB67D4BFE567A" modified="2018-07-18" version="1">MTYSEKPRSNAWFLLPVFLGIIGGIIAYFVLRNDDPRKAKNCLYLGLVLMGIGIIFNMLIAASIPGIESGFNVNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNV5</accession>
    <name>A0A2S2KNV5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thiamine-binding protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_00930</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0045 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875969.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNV5"/>
    <dbReference type="OrthoDB" id="10763at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029756">
        <property type="entry name" value="MTH1187/YkoF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002767">
        <property type="entry name" value="Thiamine_BP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051614">
        <property type="entry name" value="UPF0045_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00106">
        <property type="entry name" value="MTH1187 family thiamine-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33777">
        <property type="entry name" value="UPF0045 PROTEIN ECM15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33777:SF1">
        <property type="entry name" value="UPF0045 PROTEIN ECM15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01910">
        <property type="entry name" value="Thiamine_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89957">
        <property type="entry name" value="MTH1187/YkoF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine-binding protein" evidence="2">
        <location>
            <begin position="4"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="101" mass="11444" checksum="BE652465EFF22320" modified="2018-07-18" version="1">MIQAEISIYPMATKTTSASFYIAKAIESIQNIENLRYQVNPMGTILESDNIDVINGAAKTMMEVVHNLGIARVEVVIKIDSRRDKQVRMEEKLESIKKQMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNW6</accession>
    <name>A0A2S2KNW6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DEAD/DEAH box helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_01130</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875987.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNW6"/>
    <dbReference type="GeneID" id="76209442"/>
    <dbReference type="OrthoDB" id="6396at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120545">
        <property type="term" value="F:nucleic acid conformation isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45766">
        <property type="entry name" value="DNA ANNEALING HELICASE AND ENDONUCLEASE ZRANB3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45766:SF6">
        <property type="entry name" value="SWI_SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY A-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="2">
        <location>
            <begin position="123"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="3">
        <location>
            <begin position="409"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="574" mass="65980" checksum="306A4AFC76654217" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNZ0</accession>
    <name>A0A2S2KNZ0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00850</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875962.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNZ0"/>
    <dbReference type="GeneID" id="76209414"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="72" mass="8491" checksum="BF00EB951D4F156E" modified="2018-07-18" version="1">MYEFEKTRVGTIKMNIKNHTLDKIGSFSTVKRNLKLNGDRGRHWWGKLTDVRLNERNISTPFSFPLDEPKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNZ2</accession>
    <name>A0A2S2KNZ2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Elongation factor 2</fullName>
            <shortName evidence="10">EF-2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">fusA</name>
        <name evidence="12" type="ORF">NZNM25_00880</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875964.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNZ2"/>
    <dbReference type="GeneID" id="76209416"/>
    <dbReference type="OrthoDB" id="6290at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01681">
        <property type="entry name" value="aeEF2_snRNP_like_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01885">
        <property type="entry name" value="EF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16268">
        <property type="entry name" value="EF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16261">
        <property type="entry name" value="EF2_snRNP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01514">
        <property type="entry name" value="Elongation_Factor_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000009">
        <property type="entry name" value="116 kDa U5 small nuclear ribonucleoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000002">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00054_A">
        <property type="entry name" value="EF_G_EF_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004543">
        <property type="entry name" value="Transl_elong_EFG/EF2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00490">
        <property type="entry name" value="aEF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF3">
        <property type="entry name" value="ELONGATION FACTOR-LIKE GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10 12" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="18"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="27"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="93"/>
            <end position="97"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="147"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Diphthamide" evidence="10">
        <location>
            <position position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH33297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="730" mass="80414" checksum="2459F88CC6F15BC8" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNZ3</accession>
    <name>A0A2S2KNZ3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Acetate--CoA ligase</fullName>
            <ecNumber evidence="2 6">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_01430</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="2 6"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNZ3"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043955">
        <property type="term" value="F:3-hydroxypropionyl-CoA synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019427">
        <property type="term" value="P:acetyl-CoA biosynthetic process from acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043427">
        <property type="term" value="P:carbon fixation by 3-hydroxypropionate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05966">
        <property type="entry name" value="ACS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000001">
        <property type="entry name" value="Acetyl-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011904">
        <property type="entry name" value="Ac_CoA_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02188">
        <property type="entry name" value="Ac_CoA_lig_AcsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001208">
        <property type="entry name" value="PRK00174.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF14">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="9">
        <location>
            <begin position="16"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="7">
        <location>
            <begin position="72"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="8">
        <location>
            <begin position="515"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="NCBIfam" id="TIGR02188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH33352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="639" mass="71668" checksum="9C2525ACAD50D9B2" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP08</accession>
    <name>A0A2S2KP08_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptide methionine sulfoxide reductase MsrB</fullName>
            <ecNumber evidence="6">1.8.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Peptide-methionine (R)-S-oxide reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">msrB</name>
        <name evidence="8" type="ORF">NZNM25_01630</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(R)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:24164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12314"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit. The zinc ion is important for the structural integrity of the protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the MsrB Met sulfoxide reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.12" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876031.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP08"/>
    <dbReference type="GeneID" id="76209493"/>
    <dbReference type="OrthoDB" id="5961at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033743">
        <property type="term" value="F:peptide-methionine (R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.20:FF:000001">
        <property type="entry name" value="Peptide methionine sulfoxide reductase MsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01400">
        <property type="entry name" value="MsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028427">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002579">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00357">
        <property type="entry name" value="peptide-methionine (R)-S-oxide reductase MsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10173">
        <property type="entry name" value="METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10173:SF52">
        <property type="entry name" value="METHIONINE-R-SULFOXIDE REDUCTASE B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01641">
        <property type="entry name" value="SelR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51790">
        <property type="entry name" value="MSRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MsrB" evidence="7">
        <location>
            <begin position="9"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="131" mass="14985" checksum="9310A903CD7DF2C4" modified="2018-07-18" version="1">MTKKVTENPEEWKEKLTPEQYEICINHGTEPPFSGKYNNSKIEGYFRCVCCGEDLFSSDAKFDSGSGWPSFWKPVAEEKIEYVSDTDYGMIRTEVNCKNCGSHLGHVFDDGPKPTNQRYCINSVSLKHEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP63</accession>
    <name>A0A2S2KP63_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Thioredoxin reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">trxB</name>
        <name evidence="8" type="ORF">NZNM25_02230</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876258.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP63"/>
    <dbReference type="GeneID" id="76209557"/>
    <dbReference type="OrthoDB" id="27340at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050097">
        <property type="entry name" value="Ferredoxin-NADP_redctase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008255">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005982">
        <property type="entry name" value="Thioredox_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01292">
        <property type="entry name" value="TRX_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48105">
        <property type="entry name" value="THIOREDOXIN REDUCTASE 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00573">
        <property type="entry name" value="PYRIDINE_REDOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="7">
        <location>
            <begin position="28"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="334" mass="36561" checksum="8A398DFDACB41B60" modified="2018-07-18" version="1">MMAADASATVLETNDSDPMKPDKTKTKFDVIIIGAGPSGYTAGIYCSRAGYDTLILSGILPGGQLVNTTEVENYPGFENGIIGPDLMIEMRKQTQRMGTTIIDDEAVDVDFRRKPFKVLTASEEYEGRAIIIATGANPRKMGLEGEQKFAGKGVSYCATCDGPFFRNQEIIVVGGGDSAIEEATFLTKFGSTIHIVHRRGELRASKVMQERAMNNEKIKFHWDSVVTDIKGDQKMQQVILKNLKTNEETAIDAGGLFVAIGHEPNTKLFKDQIDLDKEGYVVLKNKTHTNVEGVFAAGDVHDRNYRQAITAAGFGCMAAIDVDKYLTESADKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP84</accession>
    <name>A0A2S2KP84_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA/RNA-binding protein Alba</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">albA</name>
        <name evidence="8" type="ORF">NZNM25_02430</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds double-stranded DNA tightly but without sequence specificity. Involved in DNA compaction.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the histone-like Alba family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_263970047.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP84"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01122">
        <property type="entry name" value="AlbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013795">
        <property type="entry name" value="DNA/RNA-bd_Alba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="3 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Alba-like" evidence="7">
        <location>
            <begin position="113"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="96"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="198" mass="21800" checksum="4F06B5D30E9EC9F3" modified="2018-07-18" version="1">MSNSKKSKDAEDILSEFDTRTKSEPTPEPEPTPEPEPTPEPEPTPEPEPTPEPEPQLSAPEPEPTPEPEPQLSAPEPEPQLSAPEPEPQLSAPEPEPQHKTSETHHPPEERDVIFVGTKPIMTYVSATLTQLSTRPTVTIKARGKRITQAVDVSQMIVKRMDSVGYVISDVRISSDSLTSQDGKQRNVSTMEIDISKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPG2</accession>
    <name>A0A2S2KPG2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Arginine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Arginyl-tRNA synthetase</fullName>
            <shortName evidence="8">ArgRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">argS</name>
        <name evidence="12" type="ORF">NZNM25_01760</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>tRNA(Arg) + L-arginine + ATP = L-arginyl-tRNA(Arg) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="8"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876044.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPG2"/>
    <dbReference type="GeneID" id="76209508"/>
    <dbReference type="OrthoDB" id="372102at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07956">
        <property type="entry name" value="Anticodon_Ia_Arg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000006">
        <property type="entry name" value="Arginyl-tRNA synthetase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="entry name" value="Arginyl tRNA synthetase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00123">
        <property type="entry name" value="Arg_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005148">
        <property type="entry name" value="Arg-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956:SF5">
        <property type="entry name" value="ARGININE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="ARGINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03485">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM01016">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="Arginyl-tRNA synthetase (ArgRS), N-terminal 'additional' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arginyl tRNA synthetase N-terminal" evidence="11">
        <location>
            <begin position="4"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="DALR anticodon binding" evidence="10">
        <location>
            <begin position="512"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH33385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="628" mass="71004" checksum="E1C8DCCE7B5C6215" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPH7</accession>
    <name>A0A2S2KPH7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Menaquinone biosynthesis decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_03430</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UbiD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876191.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPH7"/>
    <dbReference type="GeneID" id="76209683"/>
    <dbReference type="OrthoDB" id="8480at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1670.10:FF:000002">
        <property type="entry name" value="Menaquinone biosynthesis decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1670.10">
        <property type="entry name" value="UbiD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022390">
        <property type="entry name" value="HBDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002830">
        <property type="entry name" value="UbiD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049381">
        <property type="entry name" value="UbiD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049383">
        <property type="entry name" value="UbiD-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048304">
        <property type="entry name" value="UbiD_Rift_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03701">
        <property type="entry name" value="mena_SCO4490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00148">
        <property type="entry name" value="UbiD family decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30108">
        <property type="entry name" value="3-OCTAPRENYL-4-HYDROXYBENZOATE CARBOXY-LYASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30108:SF17">
        <property type="entry name" value="FERULIC ACID DECARBOXYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01977">
        <property type="entry name" value="UbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20696">
        <property type="entry name" value="UbiD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20695">
        <property type="entry name" value="UbiD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143968">
        <property type="entry name" value="UbiD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like N-terminal" evidence="3">
        <location>
            <begin position="10"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like Rift-related" evidence="2">
        <location>
            <begin position="121"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like C-terminal" evidence="4">
        <location>
            <begin position="321"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="480" mass="53539" checksum="D13D599C8461FAD5" modified="2018-07-18" version="1">MAIKDIREFISELEKHGELKRVKVQVDTDLEIAEILRREVYSNGSAILFENVKGFDIPVLGNAFGSMKRLEIGLEMTDFTEIGQRIADMTKMDIPSGILDKIKKLPELSKMTASFPKLETNGPVTEITSNNASFDDLPILKSWPNDAGRFITLGLVATKHPETGVRNLGVYRMQIIDKTHASMHWQKHKRGAHHGDISKDRGEKIPTAIIIGGEPATIFSSIAPVPEGLDKYLFAGITRKEGIKMVKCKTIDLDVPANAEIVLEGYVDPADIRDEGPFGDHTGYYTPVEPYPTFTLTGIMRRKDPIYVTTVVGKPILEDAYIGKVIERSFLPLIQMFHPEVIDFSMPAAGWFQGFAIISIKKRYPGQAKKVMMGLWGMGQLSLTKMFVVVDEDINVHDINDVIWAITTRADAARDTMIINNTPTDTLDPASPLINLGSKMGIDATQKTRDEGYEREIQQQVKVDDKTKSLVDAKWSDYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPJ7</accession>
    <name>A0A2S2KPJ7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">30S ribosomal protein S24e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_03630</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876209.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPJ7"/>
    <dbReference type="GeneID" id="76209704"/>
    <dbReference type="OrthoDB" id="9721at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053709">
        <property type="entry name" value="eRP_eS24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001976">
        <property type="entry name" value="Ribosomal_eS24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01282">
        <property type="entry name" value="Ribosomal_S24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00035358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="121" mass="13454" checksum="4C5E706701637D20" modified="2018-07-18" version="1">MSIIETITDVDNTFLSRRELTCNFAGLGGKLKSLEAADMISKEFKLDGKVVIPMRLKTHVGKSHVTGTFYVYDDEGLAKKHVNPTIFSRLEKIKAKLAEAQAAETPAEEAPTEEVKEEKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPK2</accession>
    <name>A0A2S2KPK2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">YkgJ family cysteine cluster protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03730</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876218.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPK2"/>
    <dbReference type="GeneID" id="76209715"/>
    <dbReference type="OrthoDB" id="36424at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005358">
        <property type="entry name" value="Puta_zinc/iron-chelating_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35866">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35866:SF2">
        <property type="entry name" value="YKGJ FAMILY CYSTEINE CLUSTER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03692">
        <property type="entry name" value="CxxCxxCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="21221" checksum="0E9FB8AA51BD524D" modified="2018-07-18" version="1">MEFKCVEECSQCCIEREYYPSKEFGKIGVLILPEEKEKIEKISAINKVDIKILPRIGVSEERDSLPTKILAYQLMGVEKNGNTCPFLDTASETKSPHGGFPCKIYENRPLACRAYPLIESEPITLDQKCKFCKEHGNADKNLNSEMEALVKIKDSMNAELPTIWRFATKVGEDEDQEILKTGWILEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPK3</accession>
    <name>A0A2S2KPK3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chromosome segregation protein ScpA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03770</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876222.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPK3"/>
    <dbReference type="GeneID" id="76209719"/>
    <dbReference type="OrthoDB" id="9051at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.580">
        <property type="entry name" value="Structural maintenance of chromosome 1. Chain E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003768">
        <property type="entry name" value="ScpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023093">
        <property type="entry name" value="ScpA-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33969">
        <property type="entry name" value="SEGREGATION AND CONDENSATION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33969:SF2">
        <property type="entry name" value="SEGREGATION AND CONDENSATION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="225" mass="25531" checksum="1B35793CB04E6C8F" modified="2018-07-18" version="1">MSEAQIPNSISQEPVNILFSPSSVAKKDVWEIDLIQILNLLIKILEKTGKKDLKVAGMAALSSSLIYRMKVESIFALQKAAMEKKPMYQRTDVDIELIDIPYRHESTYPVSLDDLLGLLQNLIGTIANPQSRRNRQLDIEPIVAPDFQEYFISLESIIGKYEDLVMKKISLTGFGLLQDIISELDQVDSIRCFFAILFLARDQKVDLEQIGDDIKITIIKEELTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPK4</accession>
    <name>A0A2S2KPK4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_01890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876057.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPK4"/>
    <dbReference type="GeneID" id="76209520"/>
    <dbReference type="OrthoDB" id="381963at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="62" mass="6958" checksum="3AF4431BC352F9C4" modified="2018-07-18" version="1">MKTRYKITSGIVIFFVSVIIVNMFGTTCILTGGDISKNWFGSVNGCGPLLTYTIHQYFGIFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPN9</accession>
    <name>A0A2S2KPN9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876171.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPN9"/>
    <dbReference type="GeneID" id="76209656"/>
    <dbReference type="OrthoDB" id="4260at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="99" mass="11576" checksum="AA05B58C45B8F324" modified="2018-07-18" version="1">MFHESSIRKSLDEYIKRRILEIPTEIKQTFPNIKQIWKCSDEFSFLYGYYIGKIEEGALRYLLKATRASAGGYVDTFEIRGIIETHKMELHDAIKSAIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPP1</accession>
    <name>A0A2S2KPP1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Daunorubicin resistance protein DrrA family ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_04230</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ABC transporter superfamily. Drug exporter-1 (DrugE1) (TC 3.A.1.105) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPP1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043215">
        <property type="term" value="P:daunorubicin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900753">
        <property type="term" value="P:doxorubicin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005894">
        <property type="entry name" value="DrrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025302">
        <property type="entry name" value="DrrA1/2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01188">
        <property type="entry name" value="drrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43582">
        <property type="entry name" value="LINEARMYCIN RESISTANCE ATP-BINDING PROTEIN LNRL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43582:SF2">
        <property type="entry name" value="LINEARMYCIN RESISTANCE ATP-BINDING PROTEIN LNRL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13732">
        <property type="entry name" value="DrrA1-3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="11"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="339" mass="37837" checksum="13A98BA03251DC85" modified="2018-07-18" version="1">MHDAIKALHSIETKSLTKSFGDVIAVNDVSFSVESGEIFGFLGPNGAGKSTTMMILTTLLKPTSGKALISGFDVSANPKKVRENIGYVQQETTVDEYLTGRENLLLQAKLNHIPKNEINVRIDAVLELIELSDKQHDSVVTYSGGMRKRLDIAGGLLHRPKVLFLDEPTVGLDIQTRRKIWKYLKKIHDEFEMTIFLTTHYMEEADQLCDRVGIIDGGKIQVIDSPKNMKNAMGNEVISILLEDGASQDSFLSELRKLEFVNKINVDGTKLTLFASNGTEVIPKIFQISSTLKIKITSIALTQPTLDDVFLSYTGHEIRDDDSKFNRKREHAKMKGLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPR4</accession>
    <name>A0A2S2KPR4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_02490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876112.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPR4"/>
    <dbReference type="GeneID" id="76209583"/>
    <dbReference type="OrthoDB" id="7828at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="122" mass="13810" checksum="151DB8EAACFD19F9" modified="2018-07-18" version="1">MLTIDCKDVSSIKNDLLVYVADKVAAIPTIKQHQFTLSTFNDDETLDITLVISAIKEYLDSIDEGKNFAVVSNNEKINITSISGRVIEREPVDQSELFSCTHCGFVTKYQVELNVHMRIHYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPT0</accession>
    <name>A0A2S2KPT0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03950</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876240.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPT0"/>
    <dbReference type="GeneID" id="76209739"/>
    <dbReference type="OrthoDB" id="6262at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="11235" checksum="F0973521B122FDF6" modified="2018-07-18" version="1">MTEFIPISQAKKMRSGVNVNAQVKSKGDPRTVNLKSGGTVDVCDAIIANGETEDDQMKLTLWGDDIKAVNVGDTVVITNGYTNEFKGEVSLTKGKFGQMEINPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPV3</accession>
    <name>A0A2S2KPV3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_02960</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876151.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPV3"/>
    <dbReference type="GeneID" id="76209633"/>
    <dbReference type="OrthoDB" id="3347at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="100" mass="11240" checksum="A390B797C65B96AD" modified="2018-07-18" version="1">MSIRKPVIKKPKTPKIKKPLISKRKKIVKKLPTLSIGRVKTKVSNKKSVIHKKTNSKIPRGTCYAEENCKGVILRKVTKTQCRNEGGKSWKKAGGTCEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPW5</accession>
    <name>A0A2S2KPW5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aminotransferase</fullName>
            <ecNumber evidence="7">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">aspC</name>
        <name evidence="9" type="ORF">NZNM25_03520</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="7"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829029.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPW5"/>
    <dbReference type="GeneID" id="76209692"/>
    <dbReference type="OrthoDB" id="372018at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046417">
        <property type="term" value="P:chorismate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.59.10">
        <property type="entry name" value="Chorismate mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050596">
        <property type="entry name" value="AspAT/PAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036263">
        <property type="entry name" value="Chorismate_II_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036979">
        <property type="entry name" value="CM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002701">
        <property type="entry name" value="CM_II_prokaryot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383:SF1">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01817">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00830">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48600">
        <property type="entry name" value="Chorismate mutase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51168">
        <property type="entry name" value="CHORISMATE_MUT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Chorismate mutase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="456" mass="51320" checksum="D027C7970B391DA4" modified="2018-07-18" version="1">MSDINDLRNHMDEVTLEMIKLLKIRIDIAKEIGEVKKHIGKGVTDEVREDNLRGKVISLCKEIGLDETIATKFFNFLLNESVKVQSTNKQTHFSIFLKAKSLEQQGKKIIHMEVGEPDFLPPTIVKKALEEVYEKGFLKYGQARGMPIFRDALAKYVSKKFSTNITENNILVSPGARFSIFTAITTLLNPGDEIIVIEPAWPAYKDCALNAGIKVRTINTTLEDKWEPSLEQIKSTINSNTKMIILNYPNNPTGKILSEKLQDDIIDIARKNNLYILSDEIYSQYAKTNWKSILSYNYKKSIVTQSFSKSHAMTGFRIGYAIADNEIIEKMAKLEAMCLTNVSEPIQYVAMKALEADISNNSNTVQNRLDMLTEKASKMGLDFVVPDGAMYIFARINQEGFDGIQFANSTLEKGLAIAPGEGFGNYKNFIRISACQDEKTLIEGMNILSNIMSETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPX2</accession>
    <name>A0A2S2KPX2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">siroheme decarboxylase</fullName>
            <ecNumber evidence="4">4.1.1.111</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_03880</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>siroheme + 2 H(+) = 12,18-didecarboxysiroheme + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:19093"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:60052"/>
            <dbReference type="ChEBI" id="CHEBI:140497"/>
            <dbReference type="EC" id="4.1.1.111"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Ahb/Nir family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.111" evidence="4"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876233.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPX2"/>
    <dbReference type="GeneID" id="76209730"/>
    <dbReference type="OrthoDB" id="145939at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3460">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040523">
        <property type="entry name" value="AsnC_trans_reg2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053953">
        <property type="entry name" value="NirdL-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF1">
        <property type="entry name" value="SIROHEME DECARBOXYLASE NIRL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17805">
        <property type="entry name" value="AsnC_trans_reg2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22451">
        <property type="entry name" value="NirdL-like_HTH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Siroheme decarboxylase NirL-like HTH" evidence="7">
        <location>
            <begin position="5"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="Siroheme decarboxylase AsnC-like ligand binding" evidence="6">
        <location>
            <begin position="61"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Siroheme decarboxylase NirL-like HTH" evidence="7">
        <location>
            <begin position="176"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Siroheme decarboxylase AsnC-like ligand binding" evidence="6">
        <location>
            <begin position="232"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="338" mass="39575" checksum="2F5F89BDB2C794BA" modified="2018-07-18" version="1">MDEFDKELLNEIQWTFPLVTRPFDAIAKKFDTTPKLIKDRLNHLKEIGVLRQLSAIFDTRKLGYTSSLVAMEIEDDKLEYVANQINRHPGVSHNYERDHEFNLWFTLAVPPGSDLKEELDKFNVLKGIKKVRMLPTLQLFKIGVKLDMVDDKKHEVAPTEEKKEIKNIKFNPTEEDKNFIRELQKDMEIIDEPFVKSAKNLGITEAELFEKMKYYEEIGVMRRFAAILRHRQVGFTANGMIVWKVPEDKISKVGETLGSFPQVSHCYERPTYSDWPYNVFSMIHCKTHDEANEMAKTIQKQIHVDDYKILFSSREFKKTRVEYFVENSFSLAEQVPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPX7</accession>
    <name>A0A2S2KPX7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Hydroxyglutarate oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_04900</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the L2HGDH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPX7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047545">
        <property type="term" value="F:(S)-2-hydroxyglutarate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43104">
        <property type="entry name" value="L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43104:SF2">
        <property type="entry name" value="L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="6">
        <location>
            <begin position="10"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="440" mass="49454" checksum="9AA7480A62A1615B" modified="2018-07-18" version="1">MCETMVHDFDIVIIGGGILGTSISYFLSQLNKSKKIAVVEQAHSVAFHTSGRNTGKVHAPYLYNPEKKKLFAKAAFHGYEMWEEYSKLRDLPFKKDGVIEVALDKKGTAVLEKYLKWGKQNGLEDKDIKLMDNVELKKIEPEIKCESALYVYKDGSVDYSVFTNSVMKDSKANGTNFILDTKVTKIKKENNKWAITLNGEHEICTKFLINAAGGEAINIAHDVGVAKKFTDVHFRGEYWRAPKEYHDLTKTSVYSVPEFPDYPFLDPHWIIRIDGSCEIGPNAVPVFSPYGYNKSENIKEFIPKILEMLGSGARKAIFDKQFQELAINEIQSSMSKTTMVNRVKRFLPKIDPDKITEKGTAGIRSSVIDDDGKFVSDVILVDDETSFHILNYNSPGATGALPFAVHIINHLNKTGLFESETVDAQCGPWNFSQILEKLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPZ1</accession>
    <name>A0A2S2KPZ1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">D-aminoacyl-tRNA deacylase</fullName>
            <ecNumber evidence="4">3.1.1.96</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">dtdA</name>
        <name evidence="5" type="ORF">NZNM25_05270</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">D-aminoacyl-tRNA deacylase with broad substrate specificity. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a D-aminoacyl-tRNA + H2O = a tRNA + a D-alpha-amino acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13953"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:10124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59871"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:79333"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>glycyl-tRNA(Ala) + H2O = tRNA(Ala) + glycine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53744"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:13640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DtdA deacylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.96" evidence="4"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876388.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPZ1"/>
    <dbReference type="GeneID" id="76209878"/>
    <dbReference type="OrthoDB" id="9863at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051499">
        <property type="term" value="F:D-aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106026">
        <property type="term" value="F:Gly-tRNA(Ala) deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019478">
        <property type="term" value="P:D-amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10700">
        <property type="entry name" value="AF0625-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.50">
        <property type="entry name" value="AF0625-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00562">
        <property type="entry name" value="Deacylase_DtdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018033">
        <property type="entry name" value="Deacylase_DtdA_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007508">
        <property type="entry name" value="DtdA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34667">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34667:SF1">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04414">
        <property type="entry name" value="tRNA_deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016210">
        <property type="entry name" value="UCP016210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142535">
        <property type="entry name" value="AF0625-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="258" mass="28836" checksum="991A1BBAE5F9799E" modified="2018-07-18" version="1">MELLVAYQDDPAGHNMAKFLSKEMTKDGDIFHGKYYDLMIIPTPAISADWLEEKYDYDGFVFLSKHAAESGQLALTCHSTGNFSEAKFGGRDREIAIPHPNLQKKYLQTLKKNQPKFPEFQITIEATHHGPSALTKPSIFIEIGTTEKQWTDESLCNSVASLVHQVMSEPIPENPVAVCFGGTHYPSKFTDELLDGKYALGTVIPKHALENLDTELFSHIISQNKMATAALLDWRGLGSDKQKILDLLKTTNLEVIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ01</accession>
    <name>A0A2S2KQ01_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866807.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ01"/>
    <dbReference type="GeneID" id="76209667"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="139" mass="16519" checksum="D992F53E0A69EA19" modified="2018-07-18" version="1">MINPELLKLLETSDVLNMLDDSVSYQLQKIQNVQRTNEGRDWYDELPKIVKGKFDSYKEDYEHLSQILKLESEQMRVEMNKGYYFWRLLRSACHTYRNDLIEYDQQLSQEFHLPETEAISDNAVLNDCIGVLDQHAIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ21</accession>
    <name>A0A2S2KQ21_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04380</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829032.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ21"/>
    <dbReference type="GeneID" id="76209787"/>
    <dbReference type="OrthoDB" id="548at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="58" mass="6740" checksum="C05D7C7D32355FA5" modified="2018-07-18" version="1">MTHGIFTQETCTHNAKVRMAGLKDMYECTNCHKFIPMIKPKEIDHAAPQYLENGWEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ37</accession>
    <name>A0A2S2KQ37_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cysteine desulfurase</fullName>
            <ecNumber evidence="3">2.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">iscS</name>
        <name evidence="11" type="ORF">NZNM25_05770</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876431.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ37"/>
    <dbReference type="GeneID" id="76209929"/>
    <dbReference type="OrthoDB" id="9577at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000003">
        <property type="entry name" value="Cysteine desulfurase IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016454">
        <property type="entry name" value="Cysteine_dSase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002806">
        <property type="entry name" value="PRK02948.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601:SF34">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601">
        <property type="entry name" value="CYSTEINE DESULFURYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005572">
        <property type="entry name" value="NifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="10">
        <location>
            <begin position="2"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH33786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="388" mass="42367" checksum="B05EF81016F02304" modified="2018-07-18" version="1">MIYLDNAASTKIHESVLDSMLPYLKEQYGNPSSIHRYGRLTHKAIEKARKQIASLINAEPSEILITSGGTESNNTALCGIAMKNPACKIITSLIEHDAILEPCKKLSQNGFEIDYLPVDSFGMVNYSELENRISEKTCLVSIMFGNNEVGTIQPISEIAKICNEKKIIFHTDAVQAVGKIPIDVKKLGIDLLSVSSHKLYGPKGVGALYIKNGISIDPVILGGGQENGLRSGTENVANIVGFGTACEIAKTCLDENMSHMKKLRDYMIKRVLSEIPEVTLNGNSEYRLPNNAHFTFLGVNGEDLIIKLDEFGIAASTGSACSVNTQKASHVLGAMGFSLEQITGSLRLTIGIFNDEKEIDQTVEVLKKVVNELRSVSPFKEKYSFGLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ52</accession>
    <name>A0A2S2KQ52_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 14">Multifunctional fusion protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="13">DNA-directed RNA polymerase subunit Rpo1C</fullName>
                <ecNumber evidence="13">2.7.7.6</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="13">DNA-directed RNA polymerase subunit A''</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="14">DNA-directed RNA polymerase subunit Rpo1N</fullName>
            </recommendedName>
            <alternativeName>
                <fullName evidence="14">DNA-directed RNA polymerase subunit A'</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="13" type="primary">rpo1C</name>
        <name evidence="14" type="synonym">rpo1N</name>
        <name evidence="14" type="synonym">rpoA1</name>
        <name evidence="13" type="synonym">rpoA2</name>
        <name evidence="17" type="ORF">NZNM25_05700</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Forms part of the jaw domain.</text>
    </comment>
    <comment type="function">
        <text evidence="12 14">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Forms the clamp head domain.</text>
    </comment>
    <comment type="function">
        <text evidence="15">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds at least 2 Zn(2+) per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 13 15">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="13"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876426.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ52"/>
    <dbReference type="GeneID" id="76209922"/>
    <dbReference type="OrthoDB" id="371812at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06528">
        <property type="entry name" value="RNAP_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02582">
        <property type="entry name" value="RNAP_archeal_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00411">
        <property type="entry name" value="RNApol_arch_Rpo1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00863">
        <property type="entry name" value="RNApol_arch_Rpo1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012757">
        <property type="entry name" value="RPO1C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012758">
        <property type="entry name" value="RPO1N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006336">
        <property type="entry name" value="PRK08566.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02390">
        <property type="entry name" value="RNA_pol_rpoA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF32">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 13 15" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 13 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="16">
        <location>
            <begin position="208"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="459"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="461"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="463"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GBH33779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="1263" mass="140253" checksum="D87C9DE1ACA6EDA5" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ70</accession>
    <name>A0A2S2KQ70_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_05450</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876406.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ70"/>
    <dbReference type="GeneID" id="76209896"/>
    <dbReference type="OrthoDB" id="9026at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="169" mass="19086" checksum="6D0530058D8AA991" modified="2018-07-18" version="1">MFGKKPTLKEGVHVFSVKKNGEFNDFIFGVVTGVDGKKVGINGVIVNPIGLKNKIEQGKTGERSREILEHPTPDNVVLALVYRVEHENFTGVLDLDVDKCDILPPKIYAMLDGWIRESLPEFLNKVLSLPQGTERDEAKRVLRNRMDTLIDKNLKRTLYAVCRSLKILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ91</accession>
    <name>A0A2S2KQ91_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ91"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="178" mass="21081" checksum="6F9EFF6BDAF69556" modified="2018-07-18" version="1">MMTEGLKTTLRYYGISPWEIEVLYGFLNSHFTVIQDEIEQDDSDFVSSLDVEIPLAFNEEFFQWFDFKRWEKVKAVFKEMKRRRGTNNALKVKINFSGNPKIVFSVDVEDKQWFDNAIEKIDFVLELLPHHLDPQKLPSEISEINYKFDSDSIRWRLNSASSKSKKYSFTGNSWKEIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQA5</accession>
    <name>A0A2S2KQA5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NAD(P)H-quinone oxidoreductase subunit 4L</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ndhE</name>
        <name evidence="8" type="ORF">NZNM25_06300</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I subunit 4L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876478.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQA5"/>
    <dbReference type="GeneID" id="76209987"/>
    <dbReference type="OrthoDB" id="56168at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030964">
        <property type="term" value="C:NADH dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.3510:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01456">
        <property type="entry name" value="NDH1_NuoK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001133">
        <property type="entry name" value="NADH_UbQ_OxRdtase_chain4L/K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039428">
        <property type="entry name" value="NUOK/Mnh_C1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004320">
        <property type="entry name" value="PRK05715.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11434:SF16">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 4L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11434">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE SUBUNIT ND4L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00420">
        <property type="entry name" value="Oxidored_q2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="62"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="101" mass="10860" checksum="3B89C0CA481B2B29" modified="2018-07-18" version="1">MTSELVDFTLVSVALLGIGIYGLAVKRNFIRMLFAVEIIINAANLNLVAFGRFLPHSGGQTLALFSIAIAAAEVAVGLSLIIVAYRMYQNVDIADFRSLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQC0</accession>
    <name>A0A2S2KQC0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ytiA</name>
        <name evidence="8" type="ORF">NZNM25_05380</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876400.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQC0"/>
    <dbReference type="GeneID" id="76209890"/>
    <dbReference type="OrthoDB" id="116318at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005115">
        <property type="entry name" value="Gly_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30506">
        <property type="entry name" value="INNER MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30506:SF3">
        <property type="entry name" value="UPF0126 INNER MEMBRANE PROTEIN YADS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03458">
        <property type="entry name" value="Gly_transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine transporter" evidence="7">
        <location>
            <begin position="14"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine transporter" evidence="7">
        <location>
            <begin position="99"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="210" mass="22482" checksum="9239F327773924FD" modified="2018-07-18" version="1">MVDFSFPIDGFISILDYLGTIAFAVTGASKAISHKADVFGIIVLATVVGVGGGVTRDVIFGRFPTAFSDPIYVSITVITGASMFFVFAKMKKQMNVWLVFDAVGLGVFSILGASIAYHVVGLNFLPMLFGGMITAIGGGILRDVFVREIPIVFVKEVYAVASIIGIVIFYVALSSGMEIQIASIIGIVSATGIRLLAMKYHWNLPKVRET</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQE8</accession>
    <name>A0A2S2KQE8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_07010</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876535.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQE8"/>
    <dbReference type="GeneID" id="76210062"/>
    <dbReference type="OrthoDB" id="204088at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051311">
        <property type="entry name" value="DedA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="VTT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42709">
        <property type="entry name" value="ALKALINE PHOSPHATASE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42709:SF6">
        <property type="entry name" value="UNDECAPRENYL PHOSPHATE TRANSPORTER A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="VTT_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="VTT" evidence="7">
        <location>
            <begin position="44"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="182" mass="19953" checksum="6FB9D940A4D29AF6" modified="2018-07-18" version="1">MEPFDSFLVWIADFLGEHLYEGIFLAALLETIVPPIPTLAVFPTAGFLASQQGISVIGLIPMIILGAIGATLGTSAIYLIALKLGRVVLIRYLRYVRVSEKKLERVEIWFEKYGDKAVFLGRMVPVMREMISIPAGLLKMRIPKFVAYTFAGSCVWSTGTILSGYYFGEAVGLGTNVISSLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQG4</accession>
    <name>A0A2S2KQG4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ArsR family transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_06940</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876529.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQG4"/>
    <dbReference type="GeneID" id="76210054"/>
    <dbReference type="OrthoDB" id="10985at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12840">
        <property type="entry name" value="HTH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="12391" checksum="F6BA8E3B97E8D8A3" modified="2018-07-18" version="1">MQTLQHVFKVEEAEQKDSLLEILSDKYCRAILESTMDKPKSAIEITAEAGIPMSTVYRRIQTLHDNKLLKTSGIITDDGKRLFMYKSRIKGIQSRYNDGKTEVELILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQK5</accession>
    <name>A0A2S2KQK5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_07340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876596.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQK5"/>
    <dbReference type="GeneID" id="76208941"/>
    <dbReference type="OrthoDB" id="10657at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050903">
        <property type="entry name" value="Bact_Chemotaxis_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022642">
        <property type="entry name" value="CheR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000780">
        <property type="entry name" value="CheR_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022641">
        <property type="entry name" value="CheR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24422">
        <property type="entry name" value="CHEMOTAXIS PROTEIN METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24422:SF10">
        <property type="entry name" value="CHEMOTAXIS PROTEIN METHYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01739">
        <property type="entry name" value="CheR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03705">
        <property type="entry name" value="CheR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00996">
        <property type="entry name" value="CHERMTFRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00138">
        <property type="entry name" value="MeTrc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47757">
        <property type="entry name" value="Chemotaxis receptor methyltransferase CheR, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50123">
        <property type="entry name" value="CHER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheR-type methyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="272" mass="31364" checksum="D4DA83118A1FBCAB" modified="2018-07-18" version="1">MTTILECQIIDIKKIVRKKIDVNIDHYASSFLERRVQNRMNMIGIKSYAEYISFLDEDFLESFELNSTLSINVTQFFRNQTVWDVFKQKIIPEIISLSSSDHPISIWSAGCAIGNEPYSLAMMFSDMLQTNEKKFKIVANDINPTSIGIAKTGQYEFEKLKNIPNSFLSKFLEKIDDDLYKFSDDLKKTITFQVGDVASFNINHVDVILCRNMLIYYADDAKELLFKKFHKTLNDSGFLVLGMAEDVPLSMKKFFKTVDLGMKIYQKVSLDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQL3</accession>
    <name>A0A2S2KQL3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_06950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876530.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQL3"/>
    <dbReference type="GeneID" id="76210055"/>
    <dbReference type="OrthoDB" id="3205at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="393"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="487" mass="57763" checksum="E7A8897213843EEC" modified="2018-07-18" version="1">MKNTQLLSVVFSLIMFTGVTASSTAFADTDDLEDRLEDFCEMNIEQRSDFFIDYPDMVEYDEKLSVICEIEDEDERENALDDFIFDVVLARDEIENDFDDEYDELEDDRDEENDVDDKYNRHADLEDRLKYFCDMTGEEKRQLFADHPRLEQFSDRLVNYCGLSEDEREDKIDDFIREHVSDTKEHDKYDLDDMLERFCDMTNEDKRKFFDNYPRLEQFSDRILNYCEISENERDDAIDKFIAEHKDVMRDYMNEKIHDKMTDVMDYDRLCSMTASDRAAEITDVATLDRISKWCNMTPEERDDYKEHHDDIKDKVTDRIRMSDMSPRLKAMIMENHEISDEKREEIKMKYREKHGEITDELKSELKIKFKDHMTKVKIKMSDERRSAIHDRLADMKAFKAELREKASEMTDEEKQKLREEFIEKAKNIQLAWISPRTQITAGIDASDVECREGFSLVMKASNGVPMCLKSDTALKMIDRGIVVPAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQL9</accession>
    <name>A0A2S2KQL9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pyridoxamine 5'-phosphate oxidase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876601.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQL9"/>
    <dbReference type="GeneID" id="76208936"/>
    <dbReference type="OrthoDB" id="953at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024747">
        <property type="entry name" value="Pyridox_Oxase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34071">
        <property type="entry name" value="5-NITROIMIDAZOLE ANTIBIOTICS RESISTANCE PROTEIN, NIMA-FAMILY-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34071:SF2">
        <property type="entry name" value="FLAVIN-NUCLEOTIDE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12900">
        <property type="entry name" value="Pyridox_ox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="210" mass="24077" checksum="740EEB43900F5277" modified="2018-07-18" version="1">MQFVGILQIRSYDKVKEFLNEEHVGRLSSIDENGFPQIIPMNFVFLNDAIYMHSHVKGEKLENISRNNKVGFEADRELEFLPSYFEDPHNASLADTLYISVVIKGMGVFVSDREEKTLALNGLMKKYQPEGQYDPIESDMRVLDAVSIIKVVPQTIHGKYKIGQHLNPKDRMELAKNILKKNSPTSKKTLKIMGFEETSDGLRMIDEPVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQP4</accession>
    <name>A0A2S2KQP4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_06420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQP4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="96" mass="10537" checksum="D15FB505E2517AE5" modified="2018-07-18" version="1">MKFPGTCIVCNEKIEINEIGLWAKGLGVKHEKCAQINELQCSVCGGSAGCLQCEFQETCDISNVSQLCICKKCSGEKDAFISYQKSTHKKFPILNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQR1</accession>
    <name>A0A2S2KQR1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_07450</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876606.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQR1"/>
    <dbReference type="GeneID" id="76208931"/>
    <dbReference type="OrthoDB" id="57329at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1090.10:FF:000022">
        <property type="entry name" value="Iron-containing alcohol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056798">
        <property type="entry name" value="ADH_Fe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039697">
        <property type="entry name" value="Alcohol_dehydrogenase_Fe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496:SF83">
        <property type="entry name" value="HYDROXYACID-OXOACID TRANSHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25137">
        <property type="entry name" value="ADH_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA" evidence="2">
        <location>
            <begin position="8"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Fe-containing alcohol dehydrogenase-like C-terminal" evidence="3">
        <location>
            <begin position="159"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="314" mass="34711" checksum="F31118685786B583" modified="2018-07-18" version="1">MHTVRIPKVINFGENALGETEYPKNALVVTTVPPELSDKWLAKMGIQDYMLYDQVKPEPSIEDVNTVISQFKDKNPSVLIGLGGGSSMDVVKYAAPEMKKEKILIPTTFGTGAEMTTYCVLKFDGKKKLLREDRFLADMAVVDSYFMDGTPEQVIKNSVCDACAQATEGYDSKLGNDLTRTLCNQAFEILYDAIMNDKPENYPYGSMLSGMGFGNCSTTLGHALSYVFSNEGVPHGYSLSSCTTVAHKHNKSIFYDRFKEAMEKLGFDKLELKADVSEAADVVMTDRGHLDPNPIPISKEDVMKCLEDIKSGNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQS7</accession>
    <name>A0A2S2KQS7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Small ribosomal subunit protein uS2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rps2</name>
        <name evidence="7" type="ORF">NZNM25_05990</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876449.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQS7"/>
    <dbReference type="GeneID" id="76209952"/>
    <dbReference type="OrthoDB" id="371797at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000030">
        <property type="entry name" value="30S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_A">
        <property type="entry name" value="Ribosomal_uS2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023454">
        <property type="entry name" value="Ribosomal_uS2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_uS2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005707">
        <property type="entry name" value="Ribosomal_uS2_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01012">
        <property type="entry name" value="uS2_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11489">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="207" mass="23316" checksum="800DEF3F54A0DB25" modified="2018-07-18" version="1">MSQQTETTDIKKKILATGIRVGTQVKTKFMRSFITKASPEGLYMLDLDITLEKIKTAAKFINRLGAENLIVCSGRQYAETPIEKFCEMLNSKKLLGRFMPGTLTNPSLPYYIEPKLVLISDPQVDEQAIIEATNAGIPIIGIANTDNITSNLDVIIPANNRGRKALATVYWLLVRQVLIERGELKEDESMKYEIDDFETKITEEEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQU1</accession>
    <name>A0A2S2KQU1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-glutamate O-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.80</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_07180</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamyl-[protein] + S-adenosyl-L-methionine = [protein]-L-glutamate 5-O-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:24452"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:10311"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:82795"/>
            <dbReference type="EC" id="2.1.1.80"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.80" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866835.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQU1"/>
    <dbReference type="GeneID" id="76208957"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008983">
        <property type="term" value="F:protein-glutamate O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.155.10">
        <property type="entry name" value="Chemotaxis receptor methyltransferase CheR, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050903">
        <property type="entry name" value="Bact_Chemotaxis_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022642">
        <property type="entry name" value="CheR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000780">
        <property type="entry name" value="CheR_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022641">
        <property type="entry name" value="CheR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036804">
        <property type="entry name" value="CheR_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24422">
        <property type="entry name" value="CHEMOTAXIS PROTEIN METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24422:SF10">
        <property type="entry name" value="CHEMOTAXIS PROTEIN METHYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01739">
        <property type="entry name" value="CheR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03705">
        <property type="entry name" value="CheR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00996">
        <property type="entry name" value="CHERMTFRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00138">
        <property type="entry name" value="MeTrc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47757">
        <property type="entry name" value="Chemotaxis receptor methyltransferase CheR, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50123">
        <property type="entry name" value="CHER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CheR-type methyltransferase" evidence="6">
        <location>
            <begin position="10"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="271" mass="32173" checksum="3CEB28F28D66D7E8" modified="2018-07-18" version="1">MTESEINTILDKIRMEVRTKTGKNIDDFKAAFLERRIRYRMEINGIVNFDEYLKLLSTNLEEANNLYLAFSINVTQFFRDPHVWEKLEKEIIPNLINKSKVHTVYVWSCGCATGEEPYSVSITLNEALKSRDVKYEIFANDINLDAISRAKKGIYRDANLANINTERRVKYFQKLEYDQYQIKSNISNQIKYENIDMMRIAEKKFHMIFCRNVLIYYDKQRYMEIFKKFADSLTENGILILGQNESMFGTIGNKLFSTLYPKERIYQKREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQV7</accession>
    <name>A0A2S2KQV7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NADH:quinone oxidoreductase/Mrp antiporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_06290</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I subunit 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876477.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQV7"/>
    <dbReference type="GeneID" id="76209986"/>
    <dbReference type="OrthoDB" id="19089at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050586">
        <property type="entry name" value="CPA3_Na-H_Antiporter_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010227">
        <property type="entry name" value="NADH_Q_OxRdtase_chainM/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003918">
        <property type="entry name" value="NADH_UbQ_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01972">
        <property type="entry name" value="NDH_I_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42703:SF1">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42703">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01437">
        <property type="entry name" value="NUOXDRDTASE4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="326"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="397"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="437"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="479"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="8">
        <location>
            <begin position="156"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="517" mass="56606" checksum="ED48FA51E79E1DE6" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQW5</accession>
    <name>A0A2S2KQW5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Digeranylgeranylglycerophospholipid reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876686.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQW5"/>
    <dbReference type="GeneID" id="76208832"/>
    <dbReference type="OrthoDB" id="46008at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011777">
        <property type="entry name" value="Geranylgeranyl_Rdtase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050407">
        <property type="entry name" value="Geranylgeranyl_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02032">
        <property type="entry name" value="GG-red-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685:SF18">
        <property type="entry name" value="DIGERANYLGERANYLGLYCEROPHOSPHOLIPID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685">
        <property type="entry name" value="GERANYLGERANYL DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="378" mass="41769" checksum="007C0D94A50A6086" modified="2018-07-18" version="1">MYFDVVIAGGSVAGLLCAREIASKGFSVLVIEEDYEIGTPEHCGGLVSIGGLEELGIIPFRKTFDHMIELAEITSPNGNSFTINSKKQKVVEISRRELDKQIAFQAQKNGAVIKVRTSFQEITDTGIRTNEEKIDCKIFVDARGVSSLIHKDRTGILSSAQYEIYADWIQKGKVEVIFDQEKYPGFFAWIIPSGESKGKVGVAGRGINVAETLDKILEEKKNYSIIRKIFAPIWIKGPIKNFVEGKTIIIGDAAGQAKPTTAGGIFTSGMGGVYAGQAISKFLETNDKSDLEEYQKKWMDRFGKEFEKQLLARKILERIDNNTINKLFGSITPEITREISEKDDFDFHTSSIIKLLGIKGSLKTAQALVGGEIKKLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQX8</accession>
    <name>A0A2S2KQX8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">AsnC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876663.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQX8"/>
    <dbReference type="GeneID" id="76208858"/>
    <dbReference type="OrthoDB" id="8136at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="1">
        <location>
            <begin position="13"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="77" mass="8779" checksum="C77A375B99621F54" modified="2018-07-18" version="1">MHKGFILLNCDLGAEEYIVDELKQMQDVNNAYLTFGAYDVVAEVQTENQDEFERVIAAIRKLSRVVSTMTLNVIKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQZ4</accession>
    <name>A0A2S2KQZ4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07680</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQZ4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="58" mass="6776" checksum="204E67CC3D92420A" modified="2018-07-18" version="1">MVVEPTYVCSSNHEFVYLITFSVGDENKTYRVCKECSKLDYFKKFIVNKIPLISTEMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR44</accession>
    <name>A0A2S2KR44_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_09140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876749.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR44"/>
    <dbReference type="GeneID" id="76208756"/>
    <dbReference type="OrthoDB" id="4891at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="25069" checksum="5CF101C078D0EF7D" modified="2018-07-18" version="1">MKTRNAQLSLIFMSIVALSWTFFLPDIHAEPSVVIAMEKTTYSYCEKLFYTIQVSEITGDPAIIHIRDEIGKGSSAIPVPITDLQTPVPSLIAFEKEIFPVGKYFIDVKYSGSQATAEFNLVDSDAICIPELIKPIMANWLSGNISDGFLIDAFEKYIDKKLINIPVEINEKNVYDVSIPQWAKNIGYWWIEGMISDEDLGNAVNYLIDKKIISFSADTIDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR59</accession>
    <name>A0A2S2KR59_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Lysine biosynthesis protein LysW</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_09370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876771.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR59"/>
    <dbReference type="GeneID" id="85193293"/>
    <dbReference type="OrthoDB" id="159847at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13946">
        <property type="entry name" value="LysW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005906">
        <property type="entry name" value="LysW"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041070">
        <property type="entry name" value="carrier_LysW_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40393:SF2">
        <property type="entry name" value="ALPHA-AMINOADIPATE_GLUTAMATE CARRIER PROTEIN LYSW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40393">
        <property type="entry name" value="LYSINE BIOSYNTHESIS PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21344">
        <property type="entry name" value="Zn_ribbon_LysW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="55" mass="5819" checksum="B45CBB5DF28A3C40" modified="2018-07-18" version="1">MNCPECDATLNIPDDASVGEIVSCPDCGADFEISKKDGSNVELKQAESVGEDWGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRB4</accession>
    <name>A0A2S2KRB4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_09840</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876812.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRB4"/>
    <dbReference type="GeneID" id="76208684"/>
    <dbReference type="OrthoDB" id="375808at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="120" mass="13322" checksum="A39497231262F912" modified="2018-07-18" version="1">MKKIKFANNILGLSLITLLSFSILSTSVFGETPKNPNVHSIKIQYVTKVSDNSYYVNFKACIGKNHVNTPTFAVTTDTNSKFITYDKIHLANTCKTYDASVNAKHTSSISIQMIDTVVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRD5</accession>
    <name>A0A2S2KRD5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_10310</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876851.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRD5"/>
    <dbReference type="GeneID" id="76208634"/>
    <dbReference type="OrthoDB" id="3060at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="142" mass="16316" checksum="0CA1D03E8E7794BF" modified="2018-07-18" version="1">MADPNFSWIYLVIFLAIPLSRIIPRLLSKKGIRFNPSKTIQERQFTPNSDSYFQKSSMDSPKPEIVPSKPQSKSMLVLGELNRGTKYFENIQKNTGLDNQELESILEDLENNGLMKVHQKQGLFGLKTELLPTDKGFKEYYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRG1</accession>
    <name>A0A2S2KRG1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_09220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRG1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="177" mass="20016" checksum="E4C3693AF0C93EC6" modified="2018-07-18" version="1">MYTNRIFNNNFINYYYEFHMLGKAKKFGIGVGIVVLVFFVVAILASIGYEAQEEKLKTPKLSEVEIKTNALKIPYDDLLRNNENYVGEIVSYQGKVLQVQNTFGDTYVMRIGVTKNTFAYDDVVWVNYAGSRVLEGDIVEFWGDVKGIRDYTAVLGNVVSVPEVNSMILNVVQKQGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRI2</accession>
    <name>A0A2S2KRI2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Lrp/AsnC family transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_10810</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876935.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRI2"/>
    <dbReference type="GeneID" id="76208578"/>
    <dbReference type="OrthoDB" id="376272at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="74" mass="8525" checksum="47F3C284648F8331" modified="2018-07-18" version="1">MTYEVLVTCNDGKRNYAIQRLKEFHDIKNIQQRKNPNEVLAQVKSDDKNYVMANIVQRIQDIDGISGTSVLPFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRI9</accession>
    <name>A0A2S2KRI9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">tRNA (guanine(26)-N(2))-dimethyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.216</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_10670</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.216" evidence="7"/>
    <dbReference type="EMBL" id="BGKI01000005">
        <property type="protein sequence ID" value="GBH34276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876923.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRI9"/>
    <dbReference type="GeneID" id="76208593"/>
    <dbReference type="OrthoDB" id="372177at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160104">
        <property type="term" value="F:tRNA (guanine(26)-N2)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002940">
        <property type="term" value="P:tRNA N2-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000272">
        <property type="entry name" value="tRNA (guanine(26)-N(2))-dimethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.70">
        <property type="entry name" value="N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002905">
        <property type="entry name" value="Trm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042296">
        <property type="entry name" value="tRNA_met_Trm1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631">
        <property type="entry name" value="N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631:SF3">
        <property type="entry name" value="TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02005">
        <property type="entry name" value="TRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51626">
        <property type="entry name" value="SAM_MT_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 8 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="1 8" id="KW-0820">tRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="382" mass="42558" checksum="D7E0E93028DE4397" modified="2018-07-18" version="1">MEISDESFQEIIEGKTKLLVPKKSITEKVPPKKPAFFNPKAKLNRDFSIIAYSAFLKNVQGPKIFLEGLSGIGARGLRVGSELKIEKIVINDLNPSALKLAEHSANLNNLKNIEFSEKEVCRFLSKYSKKGLRGSIVDIDPFGSPAAYFDCGIRATMHGGILSTAATDLQVLNGLFQGACKRKYGGVPVRTEYGNEIAIRLVLGCLRAVAARLGVEISPLFVESEMHYYRTYVKVLNRPDQEENLGYILHCKNCGHRKISLEQEQECNLCKQKTSIAGPLWIGKIFDKEFIQKMLLEIPNLEVDKICEKTLVKCLAESEMPATYFTLDEIASKMKGSPPKLESAILNLQKNDFVASVTSFSPTGFRTNANINEVIKIFQTIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRN6</accession>
    <name>A0A2S2KRN6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zinc-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11000</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876949.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRN6"/>
    <dbReference type="GeneID" id="56068595"/>
    <dbReference type="OrthoDB" id="6591at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="69" mass="7701" checksum="6FDC3174365B0855" modified="2018-07-18" version="1">MKIKCPKCKEDAELAMDFSVVKCGSCDFDMSYGEYVKFVAHNESKYSDILGDYTGSTEGHTSGSLDEWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRP0</accession>
    <name>A0A2S2KRP0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11330</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876976.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRP0"/>
    <dbReference type="GeneID" id="76208524"/>
    <dbReference type="OrthoDB" id="2578at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="228" mass="25292" checksum="48D1DED7A2EA4575" modified="2018-07-18" version="1">MLRKNLFFSLGFILSILLISCPSLSFGEKDTVSVESFDVHYDIENGILESIFLDPDFIELLITMNTSSDGTFEITIPRNLLDAKFDASDDVFFILVDGFETDYVEINSDSNSRTLVIPFFSGDSVIDIIGTNAFNPFSTEPEIPSWIKNNAGWWADGQIDDTAFIQGIQYLITEGIMAIPQTESGVSSGNKIPSWIKNNAGWWADGQIDDTAFIQGIQYLVTNGILDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRP4</accession>
    <name>A0A2S2KRP4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_10280</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRP4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="461"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="33"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="359"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="469"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="603" mass="68315" checksum="375113A9E7C794CA" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRP7</accession>
    <name>A0A2S2KRP7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866876.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRP7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="59" mass="6659" checksum="DCE732CB2F9BCF3D" modified="2018-07-18" version="1">MVVTIPVAFIEPSSGQVRDPPLIPLFYAAIAGIIIILVYSSYKEKQERQKANAKRRSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRQ2</accession>
    <name>A0A2S2KRQ2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876965.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRQ2"/>
    <dbReference type="GeneID" id="76208537"/>
    <dbReference type="OrthoDB" id="6527at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="103" mass="11963" checksum="75BDDE52314DDE9F" modified="2018-07-18" version="1">MNIVLEIGTKNYIDDLLSIKKSLSHMESLLACYNGYALSEPSSKFGWTFFKLAFKPEFQTGIEEKFSDMIEKYQWSSSPQKFTKFMTDYFLSRGCNVKIKLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRU0</accession>
    <name>A0A2S2KRU0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1059 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_09660</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876797.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRU0"/>
    <dbReference type="GeneID" id="76208704"/>
    <dbReference type="OrthoDB" id="9023at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009409">
        <property type="entry name" value="DUF1059"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06348">
        <property type="entry name" value="DUF1059"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="54" mass="6284" checksum="03DE19C0477F7E15" modified="2018-07-18" version="1">MTKLRCGDYGFECDFVSEGELEKVLSEFGKHTEDEHGIDYSKEALMQLILRKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRV3</accession>
    <name>A0A2S2KRV3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ASCH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877009.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRV3"/>
    <dbReference type="GeneID" id="76208478"/>
    <dbReference type="OrthoDB" id="139268at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06554">
        <property type="entry name" value="ASCH_ASC-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.30">
        <property type="entry name" value="Hypothetical protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007374">
        <property type="entry name" value="ASCH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04266">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ASCH" evidence="1">
        <location>
            <begin position="4"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="151" mass="17719" checksum="C44287B2C4C95863" modified="2018-07-18" version="1">MKCLSVSQPFADLIISGKKTIELRKWNTNFRGEFLIHAPLKIRVEDCKRLKMNKKFVTGAIIGKAEIYDVKKYNSIREIKMDQKFHLASKNFHDKTFGFRLKNAKSLRIPITCKGQLGFFDVDIPKTKIKNTEIVSEIIDEEYRYQWIGHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRZ8</accession>
    <name>A0A2S2KRZ8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">proline dehydrogenase</fullName>
            <ecNumber evidence="3">1.5.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">fadM</name>
        <name evidence="11" type="ORF">NZNM25_10190</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23784"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.5.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.2" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866855.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRZ8"/>
    <dbReference type="GeneID" id="76208648"/>
    <dbReference type="UniPathway" id="UPA00261">
        <property type="reaction ID" value="UER00373"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004657">
        <property type="term" value="F:proline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:L-proline catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008219">
        <property type="entry name" value="PRODH_bac_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002872">
        <property type="entry name" value="Proline_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015659">
        <property type="entry name" value="Proline_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914:SF0">
        <property type="entry name" value="PROLINE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914">
        <property type="entry name" value="PROLINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01619">
        <property type="entry name" value="Pro_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000196">
        <property type="entry name" value="Pro_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="FAD-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0642">Proline metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proline dehydrogenase" evidence="10">
        <location>
            <begin position="47"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="305" mass="35591" checksum="8F7E79A7496372B8" modified="2018-07-18" version="1">MTELKSYNYLQRIFTVGTGTQLMEKVLFKIAKQWIAGNTIDDALVSAKNAYQLGRHAIINKLGEYHTSKKQINADMEEYKKIIDSFRKWNIRGAISVKPTQIGLSISQKECYHNFEKIIQKACNAHVFVWLDMESAEHTDETIEIYSTFFSKYERLGIALQANLKRTEDDLNDLLQMGAKIRLVKGAYREKASISFKSKEDVDKNYLKLMKTLFKKGNEFAIATHDEKIIRKAENLSKKYPKKFEFQMLKGIREELKPKLIKKKLVVSDYIPYGINWLPYSFRRIKERKRNILLLGSSLIQSQRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS33</accession>
    <name>A0A2S2KS33_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_12500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS33"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="76" mass="8911" checksum="8ACE1E66E3D52987" modified="2018-07-18" version="1">MLNYYNEFLKYMATSKTRKVDAAELQKLQKDEKIRNDARRTGQKKGVSISTDKPKLKTEKVDRPARKTIKKRVAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS46</accession>
    <name>A0A2S2KS46_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12670</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877326.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS46"/>
    <dbReference type="GeneID" id="76209235"/>
    <dbReference type="OrthoDB" id="9120at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="116" mass="13591" checksum="6C9EC5A6C25BB006" modified="2018-07-18" version="1">MTEFVHKPYDQIYVRDMIKLDLDDLIGMMSSLEAANAYWVDGVLFASFAMTESEELAKKEMQNEMFLDKIIFSVYEKYTKTVKSSTNLEIGVLNMQKSKLYQDLIAWLKTQPIWNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS55</accession>
    <name>A0A2S2KS55_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PRC-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_12770</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877110.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS55"/>
    <dbReference type="GeneID" id="76209224"/>
    <dbReference type="OrthoDB" id="2513at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.240">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC-barrel" evidence="1">
        <location>
            <begin position="12"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="PRC-barrel" evidence="1">
        <location>
            <begin position="83"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="140" mass="15726" checksum="9A46CBADD3FF416C" modified="2018-07-18" version="1">MADENTHFSKYFSLPQFIGKSVFDSKGRDCGKIKSVLVDPQKFSISGVIVKKRLSKEYFLSRDYFEEINDSGLSLNTVPIKPGDKVADIDGKNIGKIIQINLNPDTNKLESLEIKSRFRSEIISSDRIVAVGEKIKIKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS75</accession>
    <name>A0A2S2KS75_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12550</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877095.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS75"/>
    <dbReference type="GeneID" id="76209248"/>
    <dbReference type="OrthoDB" id="4134at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="88" mass="10583" checksum="48863AD0C4937CCC" modified="2018-07-18" version="1">MDWVEMILKKSCEKTLTKSEVLHSLFHMIEVNENTLNQIQTDTRDFGPELEELKQTEIRDLEFHLKYYRSLVNYINLIPENKVIRNQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS76</accession>
    <name>A0A2S2KS76_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thaumarchaeal output domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_13310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS76"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040572">
        <property type="entry name" value="TackOD1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18551">
        <property type="entry name" value="TackOD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thaumarchaeal output" evidence="1">
        <location>
            <begin position="67"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="389" mass="44544" checksum="BBA414948713AC23" modified="2018-07-18" version="1">MRWKILFRKKNNNSKFESKPPHKIGGFDNLSSIFKGNSNEHKIDTNDEELSVNNPVSVDESDSLKPSTSKAVKLIQEVQNLKDRTIIPFVDYNEGKLFYPILSKIGEVQDNIACLDDLVSDGILEKKIYEKLIVCPIHPDTHSSSVRLYCPKCHSMNVEKLNLFEHKMCGYITESTNFDFTDPENSRCPSCKKQIKIFEKEIRVPAMWYQCIDCVEKFDNAVIKLHCRKYEHDFDTNSGQFIPTYCYKLKNSETSINSDTNQIKDDLLKLLQGFNFDAKLNISIKGKSNNVHEIPIYGKSNITNESILIFIKNHPDGINQSDMNYVLVPKLDIDPEKTLLLTISGINEGVENLANHYGIHLISESDFSQIIARVEKFVSEWYSENGGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS86</accession>
    <name>A0A2S2KS86_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Phosphoglucosamine mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">NZNM25_11160</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876962.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS86"/>
    <dbReference type="GeneID" id="76208541"/>
    <dbReference type="OrthoDB" id="10363at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016868">
        <property type="term" value="F:intramolecular phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43771">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43771:SF1">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="8">
        <location>
            <begin position="6"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="9">
        <location>
            <begin position="138"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="10">
        <location>
            <begin position="252"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase C-terminal" evidence="7">
        <location>
            <begin position="369"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="432" mass="47747" checksum="B6D98B6BE273A8F9" modified="2018-07-18" version="1">MKKTISGVRGIFGEDLNLKDVLEFCNNFSSLIKSQKCVIGKDTRPSGSMIKNTASAELMKNGINVYNLETVPTPVVFREARKYGAGLVVSSSHNPIEWNGMKFIIEGRGINEHELPKIIEHQENSKSKIGIEQDITSTYIEDAKKIIGDIENSPDIVIDVGGGAAKGFAPDLLRTSGCNVEVLNEDLSQCSRGPDPTSDELFELIAASMKKEIGFAFDLDGDRLVVVRNGEKQTPDVTLGLGVAKSLELGYKDFVLSIDTSVSIEKFIKEKGGSVKRSKVGEANVIEMMLKTNAQAGGEGSSGGFILPEFNYCREGILTSGLIASMLGSPKFNEILNYMKNYFQIREKTNVDSNFHDKVIEEIKNKFSKEYSEVNTQDGIKGIIDEDSWILIRKSNTEDIIRISAESNNEDKCKKITKKTLEIVKQSYDKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSC8</accession>
    <name>A0A2S2KSC8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclophilin TM1367-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_13150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877139.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSC8"/>
    <dbReference type="GeneID" id="76209184"/>
    <dbReference type="OrthoDB" id="8073at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025658">
        <property type="entry name" value="Cyclophilin_TM1367"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04126">
        <property type="entry name" value="Cyclophil_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclophilin TM1367-like" evidence="1">
        <location>
            <begin position="16"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="131" mass="14218" checksum="D98A5C5B4C1314D1" modified="2018-07-18" version="1">MYELSSPSVSKKQLILEIRGKGKISCDLKRHLSPRTVGTIMRSLPLEGNAHLMGQSILYFETSVDSGIERARSEFKKGDVAFLPSSGSVCFFINDVSSGKTMTPIGKLGDDVDVLKTAKSGDVLCIYEDTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSD1</accession>
    <name>A0A2S2KSD1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-grasp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_12680</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the D-alanine--D-alanine ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877104.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSD1"/>
    <dbReference type="GeneID" id="76209234"/>
    <dbReference type="OrthoDB" id="1018at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008716">
        <property type="term" value="F:D-alanine-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011095">
        <property type="entry name" value="Dala_Dala_lig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23132">
        <property type="entry name" value="D-ALANINE--D-ALANINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23132:SF23">
        <property type="entry name" value="D-ALANINE--D-ALANINE LIGASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07478">
        <property type="entry name" value="Dala_Dala_lig_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="5">
        <location>
            <begin position="122"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="722" mass="80816" checksum="DB2BB68C25003010" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSF9</accession>
    <name>A0A2S2KSF9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11860</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877038.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSF9"/>
    <dbReference type="GeneID" id="76209320"/>
    <dbReference type="OrthoDB" id="4894at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="124" mass="14826" checksum="3AAB06546325D77A" modified="2018-07-18" version="1">MVTYNDHLKNILIQILESYNHLKEIKDKPGDLKNIKKEMLKINGFLKVITNKIDFENIHLSDFKPLKSKIKQYLENYSFEQEIDTMIPLYSDDIHRVKNMRLKILESLEDKKMIYDVEGLIEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSX9</accession>
    <name>A0A2S2KSX9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_15350</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSX9"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="132" mass="15271" checksum="A1B7A136A29903B9" modified="2018-07-18" version="1">MGPFTKLFIIIYVGCFVKDIEFKINLTEIHPDSEIKGTVLVSYPGRYDGVVINTQILDSNEHIVYKSYNGKNISQNVSRLFINKDVMPENKAEFTALIKFEPKQEHEVKFRASIIEQHKEIESQIIFAKYSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT08</accession>
    <name>A0A2S2KT08_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl44e</name>
        <name evidence="6" type="ORF">NZNM25_15740</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL42 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877403.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT08"/>
    <dbReference type="GeneID" id="56068754"/>
    <dbReference type="OrthoDB" id="52456at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.80:FF:000001">
        <property type="entry name" value="60S ribosomal protein L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01476">
        <property type="entry name" value="Ribosomal_L44e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000552">
        <property type="entry name" value="Ribosomal_eL44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053708">
        <property type="entry name" value="Ribosomal_LSU_eL42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004425">
        <property type="entry name" value="PRK05767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10369">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36A/L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00935">
        <property type="entry name" value="Ribosomal_L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="zinc finger region" description="C4-type" evidence="4">
        <location>
            <begin position="11"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="93" mass="10579" checksum="E052BDED0B1CC6B6" modified="2018-07-18" version="1">MNIPKVIRKYCAKCKTHTEQKVSIYKAGKRRGSARGERRHAERKKGYGGQKFPKLAKPAKVTKKVTPIMTCTVCKKKYNKVGVRIKKFELVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT09</accession>
    <name>A0A2S2KT09_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 14">tRNA (cytidine(56)-2'-O)-methyltransferase</fullName>
            <ecNumber evidence="5 14">2.1.1.206</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 14">tRNA ribose 2'-O-methyltransferase aTrm56</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">NZNM25_16010</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 14">Specifically catalyzes the AdoMet-dependent 2'-O-ribose methylation of cytidine at position 56 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>cytidine(56) in tRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(56) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42968"/>
            <dbReference type="Rhea" id="RHEA-COMP:10308"/>
            <dbReference type="Rhea" id="RHEA-COMP:10309"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.206"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4 14">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the aTrm56 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.206" evidence="5 14"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877439.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT09"/>
    <dbReference type="GeneID" id="76208385"/>
    <dbReference type="OrthoDB" id="14397at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106059">
        <property type="term" value="F:tRNA (cytidine(56)-2'-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002128">
        <property type="term" value="P:tRNA nucleoside ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18083">
        <property type="entry name" value="aTrm56-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00077">
        <property type="entry name" value="tRNA_methyltr_aTrm56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002845">
        <property type="entry name" value="tRNA_mtfrase_aTrm56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42197">
        <property type="entry name" value="TRNA (CYTIDINE(56)-2'-O)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42197:SF1">
        <property type="entry name" value="TRNA (CYTIDINE(56)-2'-O)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01994">
        <property type="entry name" value="Trm56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016123">
        <property type="entry name" value="UCP016123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 14 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="9 14 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="11 14" id="KW-0819">tRNA processing</keyword>
    <feature type="binding site" evidence="14">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="108"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GBH34810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="177" mass="20436" checksum="DA5D6F2A77A0C276" modified="2018-07-18" version="1">MIIEVLRIGQRLVRDDRVTTHVALVSRAFGAERIFMNEVNPEIKDTVEKINKTWGGKFEVEFIDKWKNVVKKKKEENFKIIHLSMYGQNINNVQEELQKEKNLLIVVGAEKVPREIYELADYNVGIGSQPHSEISALAILLDRIQKGGQFEKYFANAKRKIIPTKNGKNVQVEETRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT10</accession>
    <name>A0A2S2KT10_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Adenine deaminase</fullName>
            <shortName evidence="6">Adenase</shortName>
            <shortName evidence="6">Adenine aminase</shortName>
            <ecNumber evidence="2 6">3.5.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">ade</name>
        <name evidence="9" type="ORF">NZNM25_15730</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>adenine + H2O + H(+) = hypoxanthine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:23688"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the metallo-dependent hydrolases superfamily. Adenine deaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.2" evidence="2 6"/>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877402.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT10"/>
    <dbReference type="GeneID" id="76208414"/>
    <dbReference type="OrthoDB" id="24954at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000034">
        <property type="term" value="F:adenine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006146">
        <property type="term" value="P:adenine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01518">
        <property type="entry name" value="Adenine_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006679">
        <property type="entry name" value="Adenine_deam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026912">
        <property type="entry name" value="Adenine_deam_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113:SF2">
        <property type="entry name" value="ADENINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-PHOSPHATE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13382">
        <property type="entry name" value="Adenine_deam_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0464">Manganese</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="7">
        <location>
            <begin position="60"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Adenine deaminase C-terminal" evidence="8">
        <location>
            <begin position="398"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="571" mass="63014" checksum="6B581F155A2F53F7" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT22</accession>
    <name>A0A2S2KT22_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_15850</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877412.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT22"/>
    <dbReference type="GeneID" id="76208403"/>
    <dbReference type="OrthoDB" id="4903at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="69" mass="7976" checksum="B8D52C2E257C2886" modified="2018-07-18" version="1">MNDDEKGKRFLELIDEQNNLQWSAIAKLTSLISSKWNSRELQNELESIVEKHSTITKELNSLDENSSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT43</accession>
    <name>A0A2S2KT43_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Succinate-semialdehyde dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_16030</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT43"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004030">
        <property type="term" value="F:aldehyde dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004777">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07100">
        <property type="entry name" value="ALDH_SSADH1_GabD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000010">
        <property type="entry name" value="Gamma-aminobutyraldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000012">
        <property type="entry name" value="NAD-dependent succinate-semialdehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044148">
        <property type="entry name" value="ALDH_GabD1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047110">
        <property type="entry name" value="GABD/Sad-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43217">
        <property type="entry name" value="SUCCINATE SEMIALDEHYDE DEHYDROGENASE [NAD(P)+] SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43217:SF1">
        <property type="entry name" value="SUCCINATE SEMIALDEHYDE DEHYDROGENASE [NAD(P)+] SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="4">
        <location>
            <begin position="6"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="467" mass="51361" checksum="503A1FD7A38353DF" modified="2018-07-18" version="1">MEKSLGQITTINPATSEEIKTYDTMDKNQVFALVGKARRAFPEWKKDYEKRRSYVYNLVEYLKKHKTELAKVATTEMGKALKESIGEVEKCAWALEFYADHGDSFLSDEVLNTDARKSFLTFEPLGVIGSIMPWNFPYWQALRFAAPCLMAGNVIVMKPSRVTMQSGIEIEKAFTESGMPDGIYQTIVGSVDSANHLIDSDVNAVTFTGSTNAGAKVGERAAKNLKKCVLELGGSDPFIVLDDAIIEKAAEGAVKGRFINCGQSCVASKRFFVGKNIAEEFTELFIKNASKLKVGDPMSIETDIGPISNKEGLETISGIVEDAKAKGAEVLLGGSEMDGKGFFYKPTILKNITSDMRIATEETFGPVAPITVVENESEAIKLANESEFGLGASIWTKDLAKADKMSRRIDSGIVSVNNVVISDPRIPFGGIKHSGFGRELSRYGMLEFVNLKSVRFYDNLTHHHYVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT61</accession>
    <name>A0A2S2KT61_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_14390</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877243.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT61"/>
    <dbReference type="GeneID" id="76209056"/>
    <dbReference type="OrthoDB" id="8006at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036280">
        <property type="entry name" value="Multihaem_cyt_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48695">
        <property type="entry name" value="Multiheme cytochromes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="136" mass="16056" checksum="AE1E70440C63BB83" modified="2018-07-18" version="1">MGFFSKKPSYCAICNKELSHKHKPKREWNIKGVLCGDCHFEKSKEYYEGKVRQPCVECGETQKITDLWEPRWQWDMEGLLCKKCFDNKEESHGKKKNFCSLCGGKMGLIRYNPKGHWKIEGQLCRRCWDEKKSELG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTB4</accession>
    <name>A0A2S2KTB4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">carbamoyl-phosphate synthase (glutamine-hydrolyzing)</fullName>
            <ecNumber evidence="3">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Arginine-specific carbamoyl phosphate synthetase, glutamine chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">NZNM25_14890</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CarA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTB4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="entry name" value="Carbamoyl-phosphate synthase small subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050472">
        <property type="entry name" value="Anth_synth/Amidotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009475">
        <property type="entry name" value="PRK12838.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418:SF7">
        <property type="entry name" value="CARBAMOYL-PHOSPHATE SYNTHASE SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="Carbamoyl phosphate synthetase, small subunit N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carbamoyl-phosphate synthase small subunit N-terminal" evidence="10">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="342" mass="38192" checksum="0E4E709F1F77AB39" modified="2018-07-18" version="1">MVGYTEALTDPSYSGQILTLTYPLVGNYGVPDYSIKDNDGIPKFFESEKIQVRGLVVHELSLTASHWNLAMTLDEWLYNEKIPGISGIDTRELTKKIRTGGVMMAALVVSDSEINAEDIKKQLASAPRYDSEQFMDYVSTKQEKTYGDENRSVVVVDTGAKNAIIRNIREIGYKVIRLPWDTSYEKIMSYHPKGVVLSSGPGDPQKCPDTINTAKKLIEHNVPTLGICLGAQIIGIAGNTETYKLKYGHRGQNKSCVNLENNQVYVTSQNHGYGITPESLQKSEFNLWFTNADDKTVEGIKHKKQKCIAVQFHPEAAPGPYDCKFVFEELQHLMEEGTSAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTC5</accession>
    <name>A0A2S2KTC5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_16770</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877502.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTC5"/>
    <dbReference type="GeneID" id="76208308"/>
    <dbReference type="OrthoDB" id="8087at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05121">
        <property type="entry name" value="ABC1_ADCK3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050154">
        <property type="entry name" value="UbiB_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10566">
        <property type="entry name" value="CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10566:SF113">
        <property type="entry name" value="PROTEIN ACTIVITY OF BC1 COMPLEX KINASE 7, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="471"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="496"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="112"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="517" mass="58921" checksum="8B25CF64B01157FE" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTD5</accession>
    <name>A0A2S2KTD5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pantoate kinase</fullName>
            <shortName evidence="5">PoK</shortName>
            <ecNumber evidence="5">2.7.1.169</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_16870</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Phosphorylates (R)-pantoate to form (R)-4-phosphopantoate in the CoA biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(R)-pantoate + ATP = (R)-4-phosphopantoate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28246"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61294"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.169"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the GHMP kinase family. PoK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.169" evidence="5"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877510.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTD5"/>
    <dbReference type="GeneID" id="76208299"/>
    <dbReference type="OrthoDB" id="85822at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02223">
        <property type="entry name" value="Pantoate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012043">
        <property type="entry name" value="PoK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42282:SF1">
        <property type="entry name" value="PANTOATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42282">
        <property type="entry name" value="PANTOATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016896">
        <property type="entry name" value="GHMP_arc_MJ0969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="3 5 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="6">
        <location>
            <begin position="84"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="303" mass="33621" checksum="6EAE11117528AEF1" modified="2018-07-18" version="1">MEAKAFCPAHITGFFKAHLEDSQNNLENLGSMGAGFSIKHGVTTKVKINTKNNQQSNFHITTKGYQSDKTDVSEYVLNEFLKLGEFSDTFFDIEHEILIPVGYGLGSSSAVALSLSYALDQALDTKLDKTRIGQIAHNAELNCKTGLGDVLASYHGGFEIRVKPGAPGIGSVEKILTDKITILMICFSPISTNKFIRERLSQINGMGGKMVKKLLESKNYEHFQDMSLEFAKYVDVMTPRMQKLVDELSQKNIKCGIALFGETVFTMISKEKENDVLEILHKYSDGSIIKSELDDQGARVLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTE2</accession>
    <name>A0A2S2KTE2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">CoA ester lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_16180</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877454.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTE2"/>
    <dbReference type="GeneID" id="76208368"/>
    <dbReference type="OrthoDB" id="9170at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005000">
        <property type="entry name" value="Aldolase/citrate-lyase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011206">
        <property type="entry name" value="Citrate_lyase_beta/mcl1/mcl2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32308:SF0">
        <property type="entry name" value="HPCH_HPAI ALDOLASE_CITRATE LYASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32308">
        <property type="entry name" value="LYASE BETA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G13030)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03328">
        <property type="entry name" value="HpcH_HpaI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015582">
        <property type="entry name" value="Cit_lyase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HpcH/HpaI aldolase/citrate lyase" evidence="4">
        <location>
            <begin position="6"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="284" mass="31597" checksum="B95542DE79D5121D" modified="2018-07-18" version="1">MTQLFRSLIFVPGNNPRFLEKAKKLRADIVCFDLEDSVPDNEKVNARNLIKSALKSRQSYESSIFVRTNSPTSGKISSDLKEVVQKGIDGIVIPKVNNVKEMEKIEKILSKLEKTRNLKPIQIIPSIESAEGVVNTYNIASFGKRVYAVVFGVFDLLNDLGVEYTKESEGARYSRAKIPVDAHAAGVVAIDAIWQDLKDSKGFAKDCKLGKSLGYSGKSIIHPDQISVVHKLFHPNKSEIFWAEKVCKAYLESIKKGKGATIVDGKMIDEVHFKQAKSLLEVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTH3</accession>
    <name>A0A2S2KTH3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS</fullName>
            <shortName evidence="6">tRNA(Ile2)-agm2C synthetase</shortName>
            <ecNumber evidence="6">6.3.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">tRNA(Ile2) agmatidine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tiaS</name>
        <name evidence="10" type="ORF">NZNM25_15560</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">ATP-dependent agmatine transferase that catalyzes the formation of 2-agmatinylcytidine (agm2C) at the wobble position (C34) of tRNA(Ile2), converting the codon specificity from AUG to AUA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>cytidine(34) in tRNA(Ile2) + agmatine + ATP + H2O = 2-agmatinylcytidine(34) in tRNA(Ile2) + AMP + 2 phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43608"/>
            <dbReference type="Rhea" id="RHEA-COMP:10625"/>
            <dbReference type="Rhea" id="RHEA-COMP:10626"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58145"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:83545"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TiaS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.22" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_425342917.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTH3"/>
    <dbReference type="GeneID" id="76208431"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002101">
        <property type="term" value="P:tRNA wobble cytosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04482">
        <property type="entry name" value="RPA2_OBF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01892">
        <property type="entry name" value="tRNA_Ile2_agm2C_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053870">
        <property type="entry name" value="TiaS-like_TCKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013696">
        <property type="entry name" value="TiaS_FLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024913">
        <property type="entry name" value="tRNA_Ile2__agm2C_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055394">
        <property type="entry name" value="Zn_ribbon_TiaS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40705">
        <property type="entry name" value="TRNA(ILE2) 2-AGMATINYLCYTIDINE SYNTHETASE TIAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40705:SF1">
        <property type="entry name" value="TRNA(ILE2) 2-AGMATINYLCYTIDINE SYNTHETASE TIAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08489">
        <property type="entry name" value="TiaS_FLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22641">
        <property type="entry name" value="TiaS_TCKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23783">
        <property type="entry name" value="Zn_ribbon_TiaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TiaS-like TCKD" evidence="8">
        <location>
            <begin position="9"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="TiaS FLD" evidence="7">
        <location>
            <begin position="143"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="TiaS C-terminal zinc ribbon" evidence="9">
        <location>
            <begin position="359"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="446" mass="50413" checksum="08BF4E1C02162037" modified="2018-07-18" version="1">MEKETILNIGFDDTDSPKGMCTTFLAYKIVDLLKKQKTEFLDFPRLIRFNPNIPWKTRGNGAVSMKIKTKNPSSVKSQVKNLVSKYSDVKNGANPGLVFFESETIPSDFIDFSNLALWRLINRNNAKKFAKKNHLEFFYKGNGQGLVGAIGAIGYDFHDHTLELLSYRKRSKFGKERKISPDSVKIMQEKTLPNTFNSFDTKKGRVLITPHGPDPVFYGVRGENVDSLLYATKILKSEEKLDGYMIFKSNQGTGDHLKNELTFENMSPYASGKITGMVSNTPKTVKGGHVFFKINSNDHELWCAVYKPTGITTIASNLIKGDKVCVGGGVRKASKNFPRIINLEFIDVIHLEKKLVTSNPLCKKCNKKMKSKGKNQGFQCIRCGKKALSKTSNEVSRKIQKKLYIPKMSAHRHLTRPSQRVGLINKSTKFDESQSWFCVYRNYSGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTH6</accession>
    <name>A0A2S2KTH6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HD domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_16720</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877497.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTH6"/>
    <dbReference type="GeneID" id="76208313"/>
    <dbReference type="OrthoDB" id="8895at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008832">
        <property type="term" value="F:dGTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006203">
        <property type="term" value="P:dGTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050135">
        <property type="entry name" value="dGTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045509">
        <property type="entry name" value="HD_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373:SF4">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE SAMHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19276">
        <property type="entry name" value="HD_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="54"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="411" mass="46996" checksum="E95C01098962A6DC" modified="2018-07-18" version="1">MKKNYLDIIDPIHDFIRVYEHELSIIDNPIFQRLRRIRQLSGAHLTYPAAQHTRFEHSLGVMHIASQAGNALNEKGILKSDDIEILRLSGLLHDIGHGPFSHLFEEVIQEKKFSHEDFGKKIILKSEIGDSLSKNGYDKKLVTKIAFGDSKFQYLNEIVSGALSADMMDYLLRDGYFTGAEHAKIDHKRITQSLDVHKKKLSLERSALYSFESMMHSRYQMFKAVYFHKTVRAAEVMLLEALRLSDDEFGFTTFNLNEFVNLTDEYILSTLISSKSMKLKRARQFAQDYQNRKLLKCVFERILTSPIDLKKTRTDELRSTISKKSKVEENEIFVDSSVTPSIPLAPSKNESKSIILISNENGKSSAKEMSISEIPVVSAISGFMNILRIYTHQKNRKKVEIAAKSIIGDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTL7</accession>
    <name>A0A2S2KTL7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Type I glutamate--ammonia ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_17670</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the glutamine synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877573.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTL7"/>
    <dbReference type="GeneID" id="76208218"/>
    <dbReference type="OrthoDB" id="36124at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019740">
        <property type="term" value="P:nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004809">
        <property type="entry name" value="Gln_synth_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027302">
        <property type="entry name" value="Gln_synth_N_conserv_site"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00653">
        <property type="entry name" value="GlnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43407">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43407:SF1">
        <property type="entry name" value="LENGSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00180">
        <property type="entry name" value="GLNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51986">
        <property type="entry name" value="GS_BETA_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS beta-grasp" evidence="10">
        <location>
            <begin position="27"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="GS catalytic" evidence="11">
        <location>
            <begin position="121"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="375"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="377"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH34976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="486" mass="55065" checksum="FA99E2BDC154F98B" modified="2018-07-18" version="1">MPYKVSHGKAIQVTYSPDEVFSRIQHEGIQFIDLQFTGLTGHFHHTTVSADTFTPEQMRDGLPKLDGSSIVGFANINDSDLLLKPDPNTFAIIPWMTENKTARLLCDIYWGENRGRLSRDPRGISQKAEEYIKSQGFDFSTWGPEVEFFVFDRVHWDVLTPYKGQSYSIESKEAPWSQEGDGYPMGLQEGYYPSTPSDTLTPYRNECVNILKNNFGILCDNHHHEVATAGQCEIDIKYDYLTNAADAAQSYKYVIRNVAQKYGKVATMMPKPIAMDSGSGMHVNVSLWKGKENAFFDPDDEIELSQIGRYFCGGIINHAKALSAICNPTTNSYHRLVPGYEAPAYIAWSSGNRSAIVRVPKHLKGKNYSYLKRLEFRAPDPSSNPYLVFAAVTAAGMDGIKKKMDPGDEVRDDIFKMTKSDRSRRGIGVLPKSLGEALDELESDRKFLNTIYTNDVIDKIIELERRDQREIAIRPHPHEFYLYFDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTM9</accession>
    <name>A0A2S2KTM9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ORC1-type DNA replication protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_17770</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in regulation of DNA replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the CDC6/cdc18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877582.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTM9"/>
    <dbReference type="GeneID" id="76208208"/>
    <dbReference type="OrthoDB" id="195574at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08768">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18139">
        <property type="entry name" value="HLD_clamp_RarA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000502">
        <property type="entry name" value="ORC1-type DNA replication protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000073">
        <property type="entry name" value="ORC1-type DNA replication protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000930">
        <property type="entry name" value="ORC1-type DNA replication protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01407">
        <property type="entry name" value="ORC1_type_DNA_replic_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049945">
        <property type="entry name" value="AAA_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015163">
        <property type="entry name" value="Cdc6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055237">
        <property type="entry name" value="Cdc6_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014277">
        <property type="entry name" value="Orc1/Cdc6_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02928">
        <property type="entry name" value="orc1/cdc6 family replication initiation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF26">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22703">
        <property type="entry name" value="Cdc6_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09079">
        <property type="entry name" value="WHD_Cdc6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01074">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NACHT" evidence="6">
        <location>
            <begin position="58"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="68"/>
            <end position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="400" mass="45045" checksum="4FED2DB442A06ED4" modified="2018-07-18" version="1">MSDPIDRMLDAAESGKSIIKNRDILHFTYIPNTILHRNSEQEKVTQSLLPILKQSRPSNLLVYGKPGTGKTLVVKKVLSKIQERVEKSNFPIKLVYSNSKDETTLYGLLVSLGRQLDLSEKELPSSGLATSEVFKRLLNKIDEGKLNAIFVIDEIDHLAQLVAKTGKDILYQLTRANERIKQGSLTLVGISNDLTFKEKLDPRVISSLGEEEIVFTNYNVEQIKKILEERIHESFIENSVEEPALNLCAALAGGEHGDARRAIDLIRVAGEIAERQQSEKVTIEHVREATQKIEENKEETSLKSYPLHEKLLILAIMKANGSSTGEIYSSYKNLCKIVGRDELTQRRITQMLSEIELSGIITGRLIHQGIHGRTKKYKLTVSSEMIKKTFKDDLTLQDIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTQ5</accession>
    <name>A0A2S2KTQ5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thiamine biosynthesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_18250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTQ5"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002937">
        <property type="term" value="P:tRNA 4-thiouridine biosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2130.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050102">
        <property type="entry name" value="tRNA_sulfurtransferase_ThiI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43209">
        <property type="entry name" value="TRNA SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43209:SF1">
        <property type="entry name" value="TRNA SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="390" mass="44300" checksum="9AB0959DA4519C31" modified="2018-07-18" version="1">MITRNFILNYKQVYFNLKNMAEISYVVVFPTIFSKNKIPQLITNIKKILKIKNQQFKSVKRDDNIILVDANDPVFASSAINLLFGIEKIAIARQIKNDFQGIVSEITDVGGNLLLKGEKFLVKVEGTSKGFLTKDVEIAATSNIIERKQNLGAHPGTDENHDKILYSYLTKNNAYICIFSDKGNNGIPYDVESQKTICAIYDELSAVSCFETIKQGNDVRIIICYRQKSELQNLAKIINQILPRLAQDNVELEFFELKIKPTGIKNYLIYVNSILEIMLQYSYNRISLALSPLVFSSDFIDNSLKSVFEKKKIPIIPLSGVDVNLFDDAKEIGLERNLKKLENIVTITSNEVPDFAKKEMEFAIRTKKKISVKVGPNNVHDILDSFEENH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTR4</accession>
    <name>A0A2S2KTR4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_16460</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877472.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTR4"/>
    <dbReference type="GeneID" id="76208339"/>
    <dbReference type="OrthoDB" id="11063at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006825">
        <property type="term" value="P:copper ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046688">
        <property type="term" value="P:response to copper ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032694">
        <property type="entry name" value="CopC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007348">
        <property type="entry name" value="CopC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008457">
        <property type="entry name" value="Cu-R_CopD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014755">
        <property type="entry name" value="Cu-Rt/internalin_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34820">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YEBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34820:SF4">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YEBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04234">
        <property type="entry name" value="CopC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05425">
        <property type="entry name" value="CopD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24684">
        <property type="entry name" value="Vgb_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0186">Copper</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="291"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="334"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="420"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="896"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="domain" description="CopC" evidence="10">
        <location>
            <begin position="22"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Copper resistance protein D" evidence="11">
        <location>
            <begin position="331"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH34855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="928" mass="102934" checksum="8F0B77A34FD45873" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTS2</accession>
    <name>A0A2S2KTS2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_18610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877685.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTS2"/>
    <dbReference type="GeneID" id="76210089"/>
    <dbReference type="OrthoDB" id="2235at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="204" mass="23283" checksum="32D682F2D606F836" modified="2018-07-18" version="1">MGKGDFKEKLIVIGFLLGVFLPLRLLFVEYVSDQWIGNFGLVSGLGIILLILIKKGKLGRIGIIFEKQMKKTIFGKTGKFVIGISVLSLLYFGSTMFFMERGESVFSEDKALFYDAIVLKNDFEQITPNNLKNIKIIYDSDNRLYSTFDYAFSITYAIMNEFSGGWLEHLYFILLVEQVEILGIFLYCRIFAKPIISMPANLTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTY7</accession>
    <name>A0A2S2KTY7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PEFG-CTERM sorting domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_19050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTY7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027560">
        <property type="entry name" value="PEFG-CTERM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04296">
        <property type="entry name" value="PEFG-CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="537"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="565" mass="62310" checksum="29C040705D8F5C78" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU03</accession>
    <name>A0A2S2KU03_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyl transferase family 28 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_19000</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877718.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU03"/>
    <dbReference type="GeneID" id="76210126"/>
    <dbReference type="OrthoDB" id="46222at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21015:SF22">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21015">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--N-ACETYLMURAMYL-(PENTAPEPTIDE) PYROPHOSPHORYL-UNDECAPRENOL N-ACETYLGLUCOSAMINE TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl transferase family 28 C-terminal" evidence="2">
        <location>
            <begin position="199"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="342" mass="38152" checksum="6B9A46EB838B8A51" modified="2018-07-18" version="1">MNVCNFFSSPIGLGHVTRDIAIVNNFENISTNFVTGGVAAEILKNLNFKVQDVYNPPSFIVENGTLKNPAKWLWSYYNYYKECKNISQKILQDDNPTLVISDEDFASLTVAQTMKIPTILVTDILETHFTKGLASLIEKKMNKSMQEISKKCDLVILPENGDDSDNIKRVGPIVRTTAFSREKLREKFLFEKKTIVVSIGGTDAGIFLIEKALESISKINQDIKIVLVSGPAVTKKFENVRNLGFVDNLHEIIFAADLLISLAGKSTIDEANAYGTPAIFIPIKGHFEQEDNALEQGYVFDDLQRLDTLILEKLEAKRKQTNTNGAKTASNIIEKLIKQSNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU04</accession>
    <name>A0A2S2KU04_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Branched-chain-amino-acid aminotransferase</fullName>
            <shortName evidence="16">BCAT</shortName>
            <ecNumber evidence="16">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ilvE</name>
        <name evidence="17" type="ORF">NZNM25_18970</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 16">Acts on leucine, isoleucine and valine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 16">
            <text>L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 16">
            <text>L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 16">
            <text>L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 16">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 16">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 15">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="16"/>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU04"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00058"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00073"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00062"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052656">
        <property type="term" value="F:L-isoleucine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052654">
        <property type="term" value="F:L-leucine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052655">
        <property type="term" value="F:L-valine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01557">
        <property type="entry name" value="BCAT_beta_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000002">
        <property type="entry name" value="D-alanine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005785">
        <property type="entry name" value="B_amino_transI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033939">
        <property type="entry name" value="BCAT_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050571">
        <property type="entry name" value="Class-IV_PLP-Dep_Aminotrnsfr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01122">
        <property type="entry name" value="ilvE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005146">
        <property type="entry name" value="PRK06606.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42743">
        <property type="entry name" value="AMINO-ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42743:SF11">
        <property type="entry name" value="AMINODEOXYCHORISMATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 16 17" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="11 16" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="10 16" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 16 17" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU364094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GBH35106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="296" mass="32753" checksum="5F61F129C9BAF43D" modified="2018-07-18" version="1">MNGKLVPFKDAKVHVLTHALHYSTSIFEGIRCYDTPSGSAIFRLPEHVDRLFKSAKLYSMKMQFTKKEISDAIIKTVKASGLKESYIRPLAYYGYGTMGLTPTENKVDVSIACWEWKMGESKAGKFSGAKCKMSSWVKIDSRSQPMQAKAASNYANAALARMEALDNGYDEAIMLNSHGKVAEGSAENIFVVKDDIIQTPPLSAGGLEGITRDSVIQIIEENSGFVIERDLERDDLYTADEIFMTGTAAEVKSVTQIDQVKIGNGKMGNITKALQKSFSDVVMGKDERFLPWLTFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU23</accession>
    <name>A0A2S2KU23_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">sulfopyruvate decarboxylase</fullName>
            <ecNumber evidence="4">4.1.1.79</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_19330</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>3-sulfopyruvate + H(+) = sulfoacetaldehyde + CO2</text>
            <dbReference type="Rhea" id="RHEA:20948"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57940"/>
            <dbReference type="ChEBI" id="CHEBI:58246"/>
            <dbReference type="EC" id="4.1.1.79"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.79" evidence="4"/>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877745.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU23"/>
    <dbReference type="GeneID" id="76210163"/>
    <dbReference type="OrthoDB" id="53192at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050545">
        <property type="term" value="F:sulfopyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019295">
        <property type="term" value="P:coenzyme M biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051818">
        <property type="entry name" value="TPP_dependent_decarboxylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42818:SF1">
        <property type="entry name" value="SULFOPYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42818">
        <property type="entry name" value="SULFOPYRUVATE DECARBOXYLASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0174">Coenzyme M biosynthesis</keyword>
    <keyword evidence="2" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="6">
        <location>
            <begin position="6"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH35142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="162" mass="18495" checksum="AC8F2BAA53F5C412" modified="2018-07-18" version="1">MKEKTQRDIFKILMKKGIENFIGVPDSTIKYFIDEGLKEKKILITTREEEAIGIASGMSLAESASLVFMQNAGFANSLSTITSLIQLYEIPMILLIGWRGFLKNDAPEHEKIGRIQQKLIKTMEIESKIIDDKNYRDVCDWAIIKLKKGELVGLIIKRDFLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU69</accession>
    <name>A0A2S2KU69_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Polysaccharide deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_19700</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877779.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU69"/>
    <dbReference type="GeneID" id="76210200"/>
    <dbReference type="OrthoDB" id="10436at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10941">
        <property type="entry name" value="CE4_PuuE_HpPgdA_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022560">
        <property type="entry name" value="DUF3473"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045235">
        <property type="entry name" value="PuuE_HpPgdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47561">
        <property type="entry name" value="POLYSACCHARIDE DEACETYLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G05030)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47561:SF1">
        <property type="entry name" value="POLYSACCHARIDE DEACETYLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G05030)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11959">
        <property type="entry name" value="DUF3473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NodB homology" evidence="1">
        <location>
            <begin position="35"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3473" evidence="2">
        <location>
            <begin position="138"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="276" mass="32092" checksum="7A49D7B37BDA983D" modified="2018-07-18" version="1">MNLLGIDFEDWYHPQLVEPFVPDAKKIPTMFKGLDKILELLRKYDIKATFFLVGELLSSNPEILDKILADQHEIGFHTMKHTRLDVPNFKPIFKEEIKEFNKITSGKSIGFRAPTFSINEKSSWVIDELADNNYKYDSSIIPAKTNLYGIPKAEKSPYKITSENLDKNDEKGSLIEFPLLVTKFLGKTIPAAGGFYLRSLPLKIIKNAIKSYERNEIPSSFYIHSWELTPEFIPKIKLPIKNRFITFHKIEKTFSKMENLFNEFDFTSFNSYFKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU73</accession>
    <name>A0A2S2KU73_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">7-cyano-7-deazaguanine synthase</fullName>
            <ecNumber evidence="9">6.3.4.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">NZNM25_18160</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the ATP-dependent conversion of 7-carboxy-7-deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>7-carboxy-7-carbaguanine + NH4(+) + 2 ATP = 7-cyano-7-carbaguanine + 2 AMP + 2 diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:27982"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the QueC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.20" evidence="9"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877617.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU73"/>
    <dbReference type="GeneID" id="76210305"/>
    <dbReference type="OrthoDB" id="6532at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01995">
        <property type="entry name" value="QueC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018317">
        <property type="entry name" value="QueC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42914">
        <property type="entry name" value="7-CYANO-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42914:SF1">
        <property type="entry name" value="7-CYANO-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06508">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006293">
        <property type="entry name" value="ExsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH35025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="224" mass="24922" checksum="C9DF7D3366B52E3F" modified="2018-07-18" version="1">MKKAVIVFSGGVDSVCAVSFLKSKYELYGITFSYGQKASNEITAAKSFAKKLGLKQHKIIDIGFMKELYGDSNVLTSSKKKIPSEFEYSIVVPIRNAVFLSIASAWAFTLNASLVAYGAHTGDTHYPDCRPLFAKKLEAAFNQGEIDGIKSKIRKNIEIWSPYRKGLSKSDLLKSGMKVLGDSIFKTWSCYSNKKYHCGICESCNNRKDAFEKAGIIDKTEYVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU78</accession>
    <name>A0A2S2KU78_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphoenolpyruvate phosphomutase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_19320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the isocitrate lyase/PEP mutase superfamily. PEP mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866940.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU78"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00377">
        <property type="entry name" value="ICL_PEPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039556">
        <property type="entry name" value="ICL/PEPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42905">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42905:SF7">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE PHOSPHOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13714">
        <property type="entry name" value="PEP_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="289" mass="32073" checksum="0241B81A73C45E94" modified="2018-07-18" version="1">MVQSKPIVKIGGAFDALSAKLVETSGFDAIWAGSFAISATHALPDASILTMTDFLRSAENMVDACNIPVIADCDTGFGGPPNVSHAVKKYEKAGIAGISIEDKIFPKQNSLLKNSRHELLPEKDFVAKIIAARESKKEDDFMIIARTEALIAEKGMDEALKRANAYENAGADAILIHSKENTPEEIFEFSKKWNSEIPLVVVPTSYPSVKLEELITHKIKMVIYANQSLRAAHNVMSKVLKDIIQKESIDKINIELSSMEEIFKLQEMYKINDQEKIIESNLKKMGYIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUA1</accession>
    <name>A0A2S2KUA1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein eS6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps6e</name>
        <name evidence="5" type="ORF">NZNM25_20240</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877840.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUA1"/>
    <dbReference type="GeneID" id="76210258"/>
    <dbReference type="OrthoDB" id="7793at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00512">
        <property type="entry name" value="Ribosomal_eS6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001377">
        <property type="entry name" value="Ribosomal_eS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020924">
        <property type="entry name" value="Ribosomal_eS6_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018282">
        <property type="entry name" value="Ribosomal_eS6_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11502">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01092">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01405">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00578">
        <property type="entry name" value="RIBOSOMAL_S6E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH35233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="132" mass="14260" checksum="64E4AA01EC608793" modified="2018-07-18" version="1">MTNFKLTISDIKGKSVSKELKDSDANPLLGLQLGNETDASIVGLSGKLKITGGSDKSGVPMRNDVHGSARKKVLLSKGVGLQDAEIGQRKRKLMRGNTVSEEIYQVNCKFDGELPVEAPAESTEEKTEDKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUD1</accession>
    <name>A0A2S2KUD1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6 7">Acylphosphatase</fullName>
            <ecNumber evidence="2 6 7">3.6.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_19920</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6 7">
            <text>an acyl phosphate + H2O = a carboxylate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="3.6.1.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the acylphosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.7" evidence="2 6 7"/>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877798.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUD1"/>
    <dbReference type="GeneID" id="76210223"/>
    <dbReference type="OrthoDB" id="6643at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003998">
        <property type="term" value="F:acylphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000012">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020456">
        <property type="entry name" value="Acylphosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036046">
        <property type="entry name" value="Acylphosphatase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017968">
        <property type="entry name" value="Acylphosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47268">
        <property type="entry name" value="ACYLPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47268:SF4">
        <property type="entry name" value="ACYLPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00708">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00112">
        <property type="entry name" value="ACYLPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54975">
        <property type="entry name" value="Acylphosphatase/BLUF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00150">
        <property type="entry name" value="ACYLPHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00151">
        <property type="entry name" value="ACYLPHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acylphosphatase-like" evidence="9">
        <location>
            <begin position="5"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="20"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH35201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="92" mass="10545" checksum="B5F1A5725667B9CC" modified="2018-07-18" version="1">MSKQRIRIFVTGRVQGVFFRQTLKAKAKQNNVYGWVKNLKDGRVEAILEGDTEKVTRLVEWAHGGPANAIVEDVEIRNEKFTGEFTKFDVAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUF8</accession>
    <name>A0A2S2KUF8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GtrA family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_18790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877702.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUF8"/>
    <dbReference type="GeneID" id="76210106"/>
    <dbReference type="OrthoDB" id="10920at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="147" mass="16970" checksum="8D4F035C7EB2EF2A" modified="2018-07-18" version="1">MLHEYLKLNKNILIAFAASIIISAIIAQILSEQTAYLNTTFTTITDYVIYFSVFSMLFYFDNRKKYRLASGRTDTAKLKHDLKKLVTSLGIAEVVYTVVRWVLQYYFLTINYDPYLASFTSQGISTIIYMLVVNLSVKITRLYKDGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNN6</accession>
    <name>A0A2S2KNN6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein uS8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rps8</name>
        <name evidence="7" type="ORF">NZNM25_00340</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875917.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNN6"/>
    <dbReference type="GeneID" id="76209361"/>
    <dbReference type="OrthoDB" id="5670at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_A">
        <property type="entry name" value="Ribosomal_uS8_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047863">
        <property type="entry name" value="Ribosomal_uS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_uS8_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003115">
        <property type="entry name" value="PRK04034.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S15A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 5" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="130" mass="14811" checksum="0E5BFBB038511BE6" modified="2018-07-18" version="1">MPATNILANLFVTLYNNETRRKRDCVILPTSKLGIEVLKTLQKDGYIGEFEHIDDKRGGKFKIKLLAKITKCGAISPRFKVKNDEYNNWEQQYLPAYDRGMLLVTTNQGVMSHHDALEKGIGGFLIGYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNW7</accession>
    <name>A0A2S2KNW7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">YwbE family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNW7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019240">
        <property type="entry name" value="DUF2196"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03833">
        <property type="entry name" value="YwbE family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40069">
        <property type="entry name" value="YWBE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40069:SF1">
        <property type="entry name" value="YWBE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09962">
        <property type="entry name" value="DUF2196"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="69" mass="7546" checksum="E2291FEB5EC1432B" modified="2018-07-18" version="1">MNLLLASIPSRDEISIGMIVQIILKQDQKTGNLTDGTVKKILTSSITHPHGIKVELNDGKIGRVQKIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNY5</accession>
    <name>A0A2S2KNY5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Flap endonuclease 1</fullName>
            <shortName evidence="10">FEN-1</shortName>
            <ecNumber evidence="10">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Flap structure-specific endonuclease 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">fen</name>
        <name evidence="13" type="ORF">NZNM25_01400</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with PCNA. PCNA stimulates the nuclease activity without altering cleavage specificity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="10"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876011.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNY5"/>
    <dbReference type="GeneID" id="76209470"/>
    <dbReference type="OrthoDB" id="9593at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09903">
        <property type="entry name" value="H3TH_FEN1-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09867">
        <property type="entry name" value="PIN_FEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000087">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000016">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00614">
        <property type="entry name" value="Fen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023426">
        <property type="entry name" value="Flap_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019973">
        <property type="entry name" value="Flap_endonuc_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03674">
        <property type="entry name" value="fen_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF9">
        <property type="entry name" value="FLAP ENDONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 10 13" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 10" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 10" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="12">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="11">
        <location>
            <begin position="140"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-domain" evidence="10">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH33349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="340" mass="38523" checksum="F4E9793F78EF1051" modified="2018-07-18" version="1">MGLNLKDLVVREKTTLEAFSTKVIAIDAYNAIYQFLASIRGPDGLQLSDSEGRITSHLSGLLYRNVNFLSLGIKPVYVFDGKPPSLKTAEIERRKQIKKDATVKYEKAIAAGNMEDARKYAQQTTSMKDGMVKESKEILTYFGIPYIDAPSEGEATAAHLTNTGQAYASASQDFDSILCGAKRLIRNFTNSGRRKIPNRNTYIDIVPEIIETEKTLQTLGLTREELIDVGILIGTDFNPNGFERIGPKTALKMIKQHSRLEDIPQIQEQLQEIDFEQIRKIFLNPEVADVEEIIFNEVDYEGLTNYLVNERSFSEERIQSTLNRLKKALEKKSQNLDQWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP22</accession>
    <name>A0A2S2KP22_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875940.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP22"/>
    <dbReference type="GeneID" id="76209391"/>
    <dbReference type="OrthoDB" id="4255at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="82" mass="9604" checksum="213C8D4F6669B40A" modified="2018-07-18" version="1">MVNFEKIYQRVALQVVQRCHGAIKITKHGKIIQVYDVKRHIWSHGLAGLIIKEECRNADLKEWEFANVRNYVIQELLGKSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP45</accession>
    <name>A0A2S2KP45_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">L-lactate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ldh</name>
        <name evidence="10" type="ORF">NZNM25_02170</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the LDH/MDH superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876084.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP45"/>
    <dbReference type="GeneID" id="76209551"/>
    <dbReference type="OrthoDB" id="2596at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004459">
        <property type="term" value="F:L-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006089">
        <property type="term" value="P:lactate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018177">
        <property type="entry name" value="L-lactate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128">
        <property type="entry name" value="L-2-HYDROXYCARBOXYLATE DEHYDROGENASE (NAD(P)(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128:SF16">
        <property type="entry name" value="L-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00086">
        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00064">
        <property type="entry name" value="L_LDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0520">NAD</keyword>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="8">
        <location>
            <begin position="2"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="141"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="6"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="113"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH33426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="302" mass="33178" checksum="542219F855E0F961" modified="2018-07-18" version="1">MISIIGSGRVGSSIAFLCASNALDDVLLINRNKDKAIGESLDITNAIPQTSEFSIHGTDDFSKLTDSDIVVIAASTGIYSKDRSENMVSQINMIKDIAEKIKRYCPSTVVLVVSNPLDVLTYFFQKESGFSRFKVIGIASSLDSSRFRYLISEKLSIPNSSILDALVLGEHGDSMVPIFSKVRVNGTSLSSMLDDDEKSVISNKVKNYWLELRKFKSRSQFGIAKNTFDVIEAIVNKKELSFPASVVLDGEYGQSNVAMGVPVRINGHGLFEIEQIELDESETEQLANSSKKIQNQINSVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP52</accession>
    <name>A0A2S2KP52_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_00920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP52"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="100" mass="10882" checksum="88157A1EDF61ADEC" modified="2018-07-18" version="1">MMKYHLLSMILVDRKILAGGLAMIIVGIILVVNISATMPAGQAGMTEEEVVDLLIAQQENQDYNTLAGILIGVGFLLVLISFGARRKKDSAKRKEKKPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPB9</accession>
    <name>A0A2S2KPB9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carboxylate--amine ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_02970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876152.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPB9"/>
    <dbReference type="GeneID" id="76209634"/>
    <dbReference type="OrthoDB" id="31247at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610:SF7">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159659">
        <property type="entry name" value="Cgl1923-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="241" mass="26983" checksum="CD41CE8888E3355A" modified="2018-07-18" version="1">MQKEFPEAEIFEIKKIELNSPIIFAGFVGAGLVGPLAINHIIEKLKMQQIAVMRSKYLPPSTVFMRGRLRHPFRFYANPEGTICAIICEITLRMEGLYSLVGEILDWAEEKGSKEIVILDGVASIGHDDKAYCAAEEDLVRTMAEKDISMIPQGFITGIPGGILNECLVREIQGLTLLAKANKVAPDSGAAATLIEALNRFYDLNIDTKQLEDEKDRFHSEFSELSQKYVEHREEIAGMYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPD1</accession>
    <name>A0A2S2KPD1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01720</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876040.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPD1"/>
    <dbReference type="GeneID" id="76209502"/>
    <dbReference type="OrthoDB" id="9907at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="116" mass="12946" checksum="5A14B838F209A7FB" modified="2018-07-18" version="1">MTELKVQGSGGYIIADLTEEQAKKADLGVGKLFLAPIGKLETEKMFKHYCNNCETEFENPPTIHLEENTNEEVADNLILVERGQYTCEKCSSVIGEYRVFKKNDESADIGKANPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPD9</accession>
    <name>A0A2S2KPD9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxamine 5'-phosphate oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_03170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876170.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPD9"/>
    <dbReference type="GeneID" id="76209654"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40660">
        <property type="entry name" value="5'-PHOSPHATE OXIDASE PUTATIVE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40660:SF1">
        <property type="entry name" value="5'-PHOSPHATE OXIDASE PUTATIVE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="PNPOx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyridoxamine 5'-phosphate oxidase N-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="146" mass="16523" checksum="A65694693314A144" modified="2018-07-18" version="1">MITPKIKEFLDLQKLGYVATVNSDGSPNLSPKGTIIGWTENTLAFANIRSPDTIKNLQSNPNIEINVIDPLLRKGYLFRGKAKILQKNLEFEKIFNHYRKNGVKSPINSIVLVDVTSVSEVTSPLYDMGMSEQEIKSKWKKHFESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPE3</accession>
    <name>A0A2S2KPE3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Digeranylgeranylglycerophospholipid reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_02550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876117.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPE3"/>
    <dbReference type="GeneID" id="76209589"/>
    <dbReference type="OrthoDB" id="6062at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011777">
        <property type="entry name" value="Geranylgeranyl_Rdtase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050407">
        <property type="entry name" value="Geranylgeranyl_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02032">
        <property type="entry name" value="GG-red-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685:SF18">
        <property type="entry name" value="DIGERANYLGERANYLGLYCEROPHOSPHOLIPID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685">
        <property type="entry name" value="GERANYLGERANYL DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="1">
        <location>
            <begin position="8"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="404" mass="44485" checksum="5C4A6BC07124CC57" modified="2018-07-18" version="1">MSEEYHYDLVVVGGGPAGSSAAYEAARNGIRVAMLEKENSIAETVRTSGVTWIQNIKEFGIPDDCYNPIKNFSFCSPNNEVTISDSVPRAAVLDVRKTYRWLAQEAKQSGADIFVKINVNNVIKDEMGDIIGVSGVGPNGKITFHSKAVIDASGFPSVVVKAMGFATQWKRFGAGAEYEVKVENIDSDTWWLMVGQQYSPAGYAWIFPLGNNIVRIGVGVGKPESNVDPTQKLKELMDSKLGPIKKLGKISPIEFHYGLIPNDGLSRKTVFNNLILVGDSAGQANPLVLEGIRYAIKFGRVAGKVASDAIKAGNTDENSLKAYEENWKKEIESKINSAGKVQDRWIGLSDEEWDKELDIIKELKPEEFIDFIKAEFGLSNMIKLATHHPKLAVRQLFNLVKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPE8</accession>
    <name>A0A2S2KPE8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Trans-sialidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_03410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866808.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPE8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="72" mass="7772" checksum="AB32F063FD929863" modified="2018-07-18" version="1">MASAKKQTKQDLENKIAELEAKLNQLSTQLEAKPAPKPAETKPAEAKPAEAKPAETKPVQLTKDLDHLVASI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPG0</accession>
    <name>A0A2S2KPG0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_02750</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPG0"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="377" mass="43619" checksum="E9C00980A9C31E4C" modified="2018-07-18" version="1">MFEMKNQKILISSLVVMLLFTLVITPIHGQTTGEGFSWQVIYLSDKTGCNVSEDRKVKDISGIIDKYFSMYKINNQRFGTTCLYSGEYFENSFSDNVDLNILVFDERIGQKLFQKYGYNGLYAHFGSDRMNNHVIMITIPPQFSSAYDNVEFPWSLSEKLSQFILSYYGYNSESITRMLDFKSDYDACVKRVVADECDKMISIIRSDISGEGYSVLSPVKEIVNQKSLKYLPDDLYSSQVVKDILRNITNWWIKGVIDDQMYLELVQQIVDVPIKNNNEIKTTQLLISNGFVIVSEEKNNSIKQKDDKIKIIQEDDEFYTVLNYVPFDANKMELEPETSEIPIWFKNRALIWQDGKLVDKIFFDGLTALIQNGFIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPG3</accession>
    <name>A0A2S2KPG3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11 12">Chorismate synthase</fullName>
            <shortName evidence="11">CS</shortName>
            <ecNumber evidence="3 11 12">4.2.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">5-enolpyruvylshikimate-3-phosphate phospholyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">aroC</name>
        <name evidence="14" type="ORF">NZNM25_03510</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 13">
            <text>5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21020"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="EC" id="4.2.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 13">
            <name>FMNH2</name>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
        </cofactor>
        <text evidence="11 13">Reduced FMN (FMNH(2)).</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11 13">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11 13">Belongs to the chorismate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.5" evidence="3 11 12"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876199.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPG3"/>
    <dbReference type="GeneID" id="76209691"/>
    <dbReference type="OrthoDB" id="33049at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00090"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004107">
        <property type="term" value="F:chorismate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07304">
        <property type="entry name" value="Chorismate_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.150.10:FF:000002">
        <property type="entry name" value="Chorismate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.150.10">
        <property type="entry name" value="Chorismate synthase AroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00300">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000453">
        <property type="entry name" value="Chorismate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035904">
        <property type="entry name" value="Chorismate_synth_AroC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020541">
        <property type="entry name" value="Chorismate_synthase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00033">
        <property type="entry name" value="aroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003793">
        <property type="entry name" value="PRK05382.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21085">
        <property type="entry name" value="CHORISMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21085:SF0">
        <property type="entry name" value="CHORISMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01264">
        <property type="entry name" value="Chorismate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001456">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103263">
        <property type="entry name" value="Chorismate synthase, AroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00787">
        <property type="entry name" value="CHORISMATE_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00788">
        <property type="entry name" value="CHORISMATE_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11 13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 11 13" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0274">FAD</keyword>
    <keyword evidence="5 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 11" id="KW-0288">FMN</keyword>
    <keyword evidence="10 11 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 11" id="KW-0521">NADP</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="126"/>
            <end position="128"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="302"/>
            <end position="306"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="NCBIfam" id="TIGR00033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GBH33560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="364" mass="39269" checksum="795384157A9F04A8" modified="2018-07-18" version="1">MPGSSIGQRLVLTSFGESHGKVIGTVLEGCPAGLAIEEKEIQKMLDLRKPGQNVISTQRKEGDVVEIVSGVFRGYTTGAPITMLIWNSDQKSKDYENLKTKLRPGHSDYPAMVKYNHYNDYRGGGRFSGRLTATHVMGGAIARKLLKVALGIETNSFTSQIGKIRMEKEFNEKMISSIYKNDVRCPENKTAKIMRDNILIAKKNGDSLGGIIESVTTNVPVGLGEPIFSSLESDLSKAIFSIPSVKGVEFGSGFRGSEMHGSENNDLYTIKKGKIVTKTNNSGGILGGISNGMPITMRIAFKPASSIAQKQSTVDIKTKKEAILQVKGRHDPCVVPRAPPVVDSLVALTIADHALIAGAIKPVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPI9</accession>
    <name>A0A2S2KPI9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Trans-sialidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_03400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876188.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPI9"/>
    <dbReference type="GeneID" id="76209679"/>
    <dbReference type="OrthoDB" id="3274at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="223"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="271"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="313" mass="34127" checksum="ECA75E5315A569DF" modified="2018-07-18" version="1">MAAKRKASTKKDLEDKIAELEAKLTKLSTQLEAKPAPKPAETKPAEAKPAEAKPAETKPAEKPAATTKPKGTLPKGFEKPAEAPKPQDAPKPAETTSQPPATVQDALENAYYTAPMTDFHQYRAKVTGYSPAPNRYFVRLTAPVGKVPTRNWNDQKATVTGYTSPSNQYFATRQRLAYHPADKRFGSFSGVNMSVEGVTAQVQSPPPEPPKPAKGTLPKGMGQPQQTPQQQTNYGGNEGKSRKELLEEYENEYLQRIEQERIEQEQAYLESVAAEAKAKSASQTKRGALPKGFEPQPEPEAPKASKGTLPKGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPK5</accession>
    <name>A0A2S2KPK5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_02160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPK5"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046666">
        <property type="entry name" value="DUF6775"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20565">
        <property type="entry name" value="DUF6775"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="256" mass="30244" checksum="82B5F91B55E703F5" modified="2018-07-18" version="1">MLLRISKIILYDEPTVLEIQLEKLEKFLRDTFPIQIEIRDNFFRNLNDEIYKKIASTRIFDIKKPFQKHNASMEDILIEKENTDTSEKEEMALYDGIELHKIISEQIPKEENNIDILNIVFTNKLTCTFDENDFRYHARALIGSNPTIISTTGIIEAPAKPKQYYLDLMTDFSKEGIDTIKKKYEGEFLEYHDSRISEAIEGYLLQAIMYYETGEAFCTDKECRLYNAHWQKELISTQLKNKKFCDKHKEILKKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPL4</accession>
    <name>A0A2S2KPL4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">yedJ</name>
        <name evidence="2" type="ORF">NZNM25_03930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876238.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPL4"/>
    <dbReference type="GeneID" id="76209735"/>
    <dbReference type="OrthoDB" id="17914at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33594:SF1">
        <property type="entry name" value="HD_PDEASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33594">
        <property type="entry name" value="SUPERFAMILY HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G03035)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="22"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="206" mass="23489" checksum="00737BE529B74CB2" modified="2018-07-18" version="1">MSVLDLLKDEVKKRIENDSAHDFDHIMRVYKNAQKICKKEKANEKLVLSAALLHDLISYPKSDKRSKMSSIESAKKSKNILKKYGFSNEEIAIISNAIRDHSFSQNKVPSSIEGKILQDADRLDALGAIGLARVFATGGSLKRPFYDPNDPFCKQRTPDDSIWTIDHFFKKLLKLESLMNTKSGKIEAKKRTIVLKNFLKQLKHEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPN0</accession>
    <name>A0A2S2KPN0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SRPBCC family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_02820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866805.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPN0"/>
    <dbReference type="GeneID" id="76209619"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07812">
        <property type="entry name" value="SRPBCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019587">
        <property type="entry name" value="Polyketide_cyclase/dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10604">
        <property type="entry name" value="Polyketide_cyc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="20204" checksum="F6BE3002D089B062" modified="2018-07-18" version="1">MANAIGTGVTVPDFRHVAHHSLFAMQLESNIFNAEELTLHMATIEVEVEINSTVDKVWEIVSDIDNEPRFWKGTKGVKNLSKEGNIIKREITIAFRDQKCLQEVKLEPKNRIEAKFTKGIINGEKIVLIIPNGENTILKTIWDIKLTGMMGMFTGMIKNHIKSGTEQAMRSIKEEIER</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPN1</accession>
    <name>A0A2S2KPN1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome-associated translation inhibitor RaiA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_02190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876086.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPN1"/>
    <dbReference type="GeneID" id="76209553"/>
    <dbReference type="OrthoDB" id="2515at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.100">
        <property type="entry name" value="Ribosome hibernation promotion factor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036567">
        <property type="entry name" value="RHF-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69754">
        <property type="entry name" value="Ribosome binding protein Y (YfiA homologue)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="12881" checksum="278704364500DE63" modified="2018-07-18" version="1">MVDHVPLYVVGLSKDEPANEILSSKFGAALEKAQQVLSHIIEAKITVEYQNPEGSRTHYDATASVKTSDKTLIYTASGWDILKIADDLSRKLEGELPKHNDNRQRESIRKREEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPN3</accession>
    <name>A0A2S2KPN3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthase</fullName>
            <shortName evidence="5">ADH synthase</shortName>
            <shortName evidence="5">ADHS</shortName>
            <shortName evidence="5">ADTH synthase</shortName>
            <ecNumber evidence="5 6">2.2.1.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">aroA'</name>
        <name evidence="8" type="ORF">NZNM25_03450</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes a transaldol reaction between 6-deoxy-5-ketofructose 1-phosphate (DKFP) and L-aspartate semialdehyde (ASA) with an elimination of hydroxypyruvaldehyde phosphate to yield 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate (ADH). Plays a key role in an alternative pathway of the biosynthesis of 3-dehydroquinate (DHQ), which is involved in the canonical pathway for the biosynthesis of aromatic amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate + L-aspartate 4-semialdehyde = 2,3-dioxopropyl phosphate + 2-amino-2,3,7-trideoxy-D-lyxo-hept-6-ulosonate</text>
            <dbReference type="Rhea" id="RHEA:25952"/>
            <dbReference type="ChEBI" id="CHEBI:58859"/>
            <dbReference type="ChEBI" id="CHEBI:58860"/>
            <dbReference type="ChEBI" id="CHEBI:58861"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="2.2.1.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodecamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DeoC/FbaB aldolase family. ADHS subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.10" evidence="5 6"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876193.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPN3"/>
    <dbReference type="GeneID" id="76209685"/>
    <dbReference type="OrthoDB" id="6329at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016744">
        <property type="term" value="F:transketolase or transaldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00958">
        <property type="entry name" value="DhnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00960">
        <property type="entry name" value="ADH_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010210">
        <property type="entry name" value="ADH_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050456">
        <property type="entry name" value="DeoC/FbaB_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041720">
        <property type="entry name" value="FbaB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01949">
        <property type="entry name" value="ADH_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005556">
        <property type="entry name" value="PRK07226.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47916:SF1">
        <property type="entry name" value="3-HYDROXY-5-PHOSPHONOOXYPENTANE-2,4-DIONE THIOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47916">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE CLASS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01791">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038992">
        <property type="entry name" value="Aldolase_Ia"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 5" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0704">Schiff base</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5 7">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with dihydroxyacetone-P" evidence="7">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="5">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="24"/>
            <end position="28"/>
        </location>
        <ligand>
            <name>1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58861"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="142"/>
            <end position="144"/>
        </location>
        <ligand>
            <name>1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58861"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="198"/>
            <end position="199"/>
        </location>
        <ligand>
            <name>1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58861"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="226"/>
            <end position="227"/>
        </location>
        <ligand>
            <name>1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58861"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="NCBIfam" id="TIGR01949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR038992-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="262" mass="28353" checksum="69835F3B094CD0D4" modified="2018-07-18" version="1">MVSGNQIRLNRILRKGRMLCIPMDHGISNGPIEGLEDPASTIYKCEGHGLTSVIINKGILKTLPKPTKVGILVHFSSSTSLSLSPNRKMLTGTVKEAVAMGADGVSLHINIGGKEEPEMLEQLGMTADQCHKWGMPLLAMMYPRGENIKDPHDPEIVAHVARIGAECGADIVKTLYTGDIESFAKIVKSTPVPIVIAGGPKTKTDLDILQMTEDAMTAGAKGITYGRNIFAHKTPEKMVEALADIIFRKVTAKEAMKKIEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPN5</accession>
    <name>A0A2S2KPN5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04310</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPN5"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="497" mass="54730" checksum="925DF24C3AD2D030" modified="2018-07-18" version="1">MLIIPIFAALLLGSISVPSAFAQFQSGGVDKEGSWYAGEGLKKGDFFSYLMCHVDYKECAKFEMDLWIKGDKQVGSETKWLAEVVVYDGNKRIVGEMELGKIAPEPTGGSAELGVYRGAFKSSVVWLSAFATSDSSSGGKGPKEFKATSWGKIGNIGGEQVLPKAIETITVPAGTWETVQMYWKTGGVGSKVWIVDDFPFPIKAKTYTHVSEGIPPTEYEFSLLEYKENVQTSPFEGIVSTLDELEAAGCEIDFEKTVGIKKPTKNFDYQVHIFYGPEEPVVGCEMDWLISFINKYDDTEYLNQVQFDFLTVDENLKPLRSIAQEEGRKFLYSPSGQHTISMKVKEDPGVARYVIWIYGLSPEGIAPSGAADYLEIEVPIFAADGTIPVQKIPDWIKNNAGWWADGSIDDNSFVQGIQFLIKEGIMKIPPTVQGSGGSANNIPSWVKNNAGWWADGSIDDNSFVQGIQFLIKEGIMKIPQSISTSKPSGYQPESLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPQ3</accession>
    <name>A0A2S2KPQ3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03650</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876211.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPQ3"/>
    <dbReference type="GeneID" id="76209706"/>
    <dbReference type="OrthoDB" id="9956at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="235" mass="26983" checksum="EDBD7A9AA2A7CBB0" modified="2018-07-18" version="1">MDPLIRFKDAHSKGIIPDDVYDLVIERFPIVVSGINRIEKASGIKYPVAYVEPSLVLSSPNPNSYEFGILFARTIPIMFDEKFQVVIQISAPLIAYGLKGTIHAILAHEFLHFLDLMRKISKMELLSDEISGNLFENIYSDETRLFEPRVVFKDRTLLNHITKKFPSGFRDYKLEDKVMKFWADKSLPKSNISLDANNVKLSAESLSRIKLAPEFISKIEQLEEKSSKITKKRLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPR6</accession>
    <name>A0A2S2KPR6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Aquaporin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_03380</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876186.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPR6"/>
    <dbReference type="GeneID" id="76209677"/>
    <dbReference type="OrthoDB" id="36050at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139">
        <property type="entry name" value="AQUAPORIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139:SF199">
        <property type="entry name" value="MIP17260P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="234" mass="24666" checksum="F724EEF3BD1950EB" modified="2018-07-18" version="1">MVNPRAYLAEAIATYGLVFFGPLSVILAIASFGEELTTISVLFISLGHGGAIALMVYTFGHVSGAHINPAVTIPMIITRKIGIADGVGYIISQLIGAITAAATLKVILPELGAKVNFATQGGPSDLINNSISSGFLIEAILTFFLVTVIFMVAVHKKASPGWHGFTIGGMVFLIHLIAVPLTGASVNPARTFGPALISGFWEFHWLYWAAPILGGIIAGLIMNYVFVKPAEKEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPS3</accession>
    <name>A0A2S2KPS3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">30S ribosomal protein S9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_04710</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876340.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPS3"/>
    <dbReference type="GeneID" id="76209823"/>
    <dbReference type="OrthoDB" id="52677at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000051">
        <property type="entry name" value="30S ribosomal protein S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_uS9_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF16">
        <property type="entry name" value="RIBOSOMAL PROTEIN S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="122"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="149" mass="16755" checksum="2C6AAABB141F0048" modified="2018-07-18" version="1">MTTPKTEIYFATRKRASAHVYITKGQGKVRINNIPVEMIPQETAREVILGPLEITGDLRDKIDISVRVRGGGFMGQASAAATGITRALIGWTKSKKDPKDHPFPKSTREDLRKRITDFDKYLVSGDARQKEPKKFGGPGARRRKQKSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPS8</accession>
    <name>A0A2S2KPS8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829033.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPS8"/>
    <dbReference type="OrthoDB" id="7663at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="438" mass="48860" checksum="EA7004590E13E21E" modified="2018-07-18" version="1">MNILVIGLLTILLGVGFSNISYGQGVQTAGGVDVEGTWYLGEGLKVGDYFEYSLCEIDLNDCAPIKLKMWIKGEIQNESESLWDTKVVVIDGNKIIQGSMGLGKTAPEPVLFDDDLFDYAIGFKSSLAWLSAFATANEDDRIHGPQKFSDAAWGKIGAIGGAQLIPKRTETINTPAGTADTVVVGWYSGNNNEIWILDDFPFPVKALTYAWVTTGIAPIMYQYTLLDYKENITDDPFKDVKSTIPKEKLLECPTEFHNYVSGRESTNTFSMLIQYNYSPEVPIEGCSIDWKINFMNKYNEVEFIDQVHYDIWVVDDAGNKIRSYAQDIGRADLFNGFGQVHILLPVEEKAGIVKYAIFVHGTGSEYEVPNPELGGYVIVEIEIAENLLLRELDNDMSYPEIPSWIKNNAGWWAAGQIGDDSFIQGIQFLIKENIIKIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPW4</accession>
    <name>A0A2S2KPW4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_05030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146195976.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPW4"/>
    <dbReference type="GeneID" id="76209855"/>
    <dbReference type="OrthoDB" id="12289at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="483" mass="54076" checksum="DD52BFD284623564" modified="2018-07-18" version="1">MMFEFTQSKITFLVGLTLFCLAFQPTVFASSSKDVALDSPFTLKIEDTAKIDSELRLTVLDAVEDSRCPSDVTCVWEGAVSVQVNLIRDDLDLGNHTILLGESEDDKQIFAGYFIKLITVEPYPSSTVPIKSSDYVLTFLVSKINEMKIDAPLKQFNTGISINNIHCKADLVLVIKSSNFSPACVTPNTAEKLSLRGWADENTLYVNPIIKTGTYAGHCIGYCAKEFLITPDKIIFSQNGRDFVSDEWSDLPEKTKEFTLSQAEWSGLVDLINFDQFRSLPDKIGCPGCADAPVEWIEISYDDKTKRIEFENGDEIPELSELIVVLREIQNRAEPIASFEECVLAGNSVMESYPRQCRTTDGKNFVEEIDDVLETPHEIEGKRSPVNVPDAISENDMWCQTSWNIETTEVLDTVYVKNSVQSTIAQFGETYFLEERIIDVFENSVGYTVSISGLWDHDSIQYQMIAEDLGKFGTIQGEPASCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPX9</accession>
    <name>A0A2S2KPX9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_05040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPX9"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="59" mass="7014" checksum="0550133BE6402929" modified="2018-07-18" version="1">MLWVNSNIIINHVIKYEIIIFDENQILPKRIKDTLFTVIMGDFRLNYKKSTKDCDLVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ27</accession>
    <name>A0A2S2KQ27_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_05670</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ27"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF8">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="410"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="444"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="284"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="289"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="301"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="552" mass="61115" checksum="0CC7EC5163C3CA0F" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ47</accession>
    <name>A0A2S2KQ47_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_05870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876439.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ47"/>
    <dbReference type="GeneID" id="76209939"/>
    <dbReference type="OrthoDB" id="11432at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="167" mass="18254" checksum="1D9DE1C9A71DD34F" modified="2018-07-18" version="1">MRRSGILIVGSGILIIAGLFTLVLGNQAILEGIVQDNGMVSSKQTLTISTDFDSQITSIGIFAVQIMEFEENTFYVKILNPFDIEIISQEINEETVEEEFDVIDSGTYKLIIQSNNNEETQVFGAIGPTPDSGKKALGFIPVYVIVVGMIGLAVVGIIEIKNRKRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ49</accession>
    <name>A0A2S2KQ49_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_05840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876437.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ49"/>
    <dbReference type="GeneID" id="76209936"/>
    <dbReference type="OrthoDB" id="12184at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049886">
        <property type="entry name" value="CFI_box_CTERM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041770">
        <property type="entry name" value="CFI_box_CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="324"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="38290" checksum="404EE7A5C2059476" modified="2018-07-18" version="1">MKMRLTAFLAFALLSVSVLSYGVNGSVFADNHNLLPVNISSNVSIIANGANVIISGTLKDYDPSALNAGAVTYVVKSPENNLVSIGQLIPNSNGSFEFSFIAGGQLWKLSGDYIVEVKYGGNTSELVLDYVGGELVVNTPPPPPPPPSTPTCTSNQQLVDGKCVDNIPPPPPPPPTCTSNQQLIDGECVDNEPPPPTCGPGTELVDGFCKVKQEPQCPTGYSMKDGLCVEEKGGCLIATAAYGTELAPQVQFLREVRDNTVLSTASGTAFMSGFNTIYYSFAPTVADWERENPMFQEAVRAFITPMLSTLSIMSLADNGSEVEVLGLGISVIALNLGMYIAAPALIGFKVHKHFKSRKEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ72</accession>
    <name>A0A2S2KQ72_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glutamate--tRNA ligase</fullName>
            <ecNumber evidence="10">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Glutamyl-tRNA synthetase</fullName>
            <shortName evidence="10">GluRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gltX</name>
        <name evidence="15" type="ORF">NZNM25_06040</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>tRNA(Glu) + L-glutamate + ATP = L-glutamyl-tRNA(Glu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="10"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876454.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ72"/>
    <dbReference type="GeneID" id="76209957"/>
    <dbReference type="OrthoDB" id="10470at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043604">
        <property type="term" value="P:amide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000037">
        <property type="entry name" value="Glutamine--tRNA ligase cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="entry name" value="Ribosomal Protein L25, Chain P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02076">
        <property type="entry name" value="Glu_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050132">
        <property type="entry name" value="Gln/Glu-tRNA_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004526">
        <property type="entry name" value="Glu-tRNA-synth_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_bL25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_bL25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049437">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00463">
        <property type="entry name" value="gltX_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003169">
        <property type="entry name" value="PRK04156.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097:SF5">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097">
        <property type="entry name" value="GLUTAMINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20974">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="Ribosomal protein L25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="12">
        <location>
            <begin position="100"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib anti-codon binding" evidence="13">
        <location>
            <begin position="408"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA synthetases class I (E and Q) anti-codon binding" evidence="14">
        <location>
            <begin position="497"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="10">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF20974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GBH33813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="569" mass="65851" checksum="267F5894E163900C" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ78</accession>
    <name>A0A2S2KQ78_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADH-quinone oxidoreductase subunit J</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">nuoJ</name>
        <name evidence="2" type="ORF">NZNM25_06310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876479.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ78"/>
    <dbReference type="GeneID" id="76209988"/>
    <dbReference type="OrthoDB" id="10566at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1200:FF:000012">
        <property type="entry name" value="NADH-quinone oxidoreductase chain 6 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1200">
        <property type="entry name" value="NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001457">
        <property type="entry name" value="NADH_UbQ/plastoQ_OxRdtase_su6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042106">
        <property type="entry name" value="Nuo/plastoQ_OxRdtase_6_NuoJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33269">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33269:SF17">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00499">
        <property type="entry name" value="Oxidored_q3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="170" mass="18342" checksum="DA4FFAFC0E696C02" modified="2018-07-18" version="1">MADAVFLALTVITIGSAIAALELRSLIYGSIALMGTLGGIAGFFFLLDSPFVALFQLAVYVGSIAVLILFTVMLVKRELIFKKIEDKRRKFAGIGLMLVVMVALGAVFLDSGIKTMTTDEPPVDFRDIGTDFVTYYWPALILMGLILAGSVTGALVLAKREDVENDQRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQA0</accession>
    <name>A0A2S2KQA0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methyltransferase type 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_06510</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876493.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQA0"/>
    <dbReference type="GeneID" id="76210009"/>
    <dbReference type="OrthoDB" id="1548at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="287" mass="33583" checksum="077D1F1470142E80" modified="2018-07-18" version="1">MEELAVKKDFTNIYTQKFPNAYLKEMKRLDYRIPDKTKPLYLSIAKQLYKKLSKKINIMDIGSSYGINSALMKYDLKMSELDDFFLEQEPSIEQSKQIFENLSYDDTFDFYQIDISEPALKFSENLGLCKKGICVNLESGNLPIKNIPRSDLIIATGCIGYIGYKAFSNLFELIKKQQSESDLEFSDKDPVFAFSVLRIFDMEKIESTFDHYDYSLVKSDLKPIRQRRFSDFEEKHKTISLLHSKGIDTKWFEDDGHFYADFYIASPKRLETSLITMSEKLKRHAEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQE9</accession>
    <name>A0A2S2KQE9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exosome complex component Rrp4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rrp4</name>
        <name evidence="8" type="ORF">NZNM25_04660</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Non-catalytic component of the exosome, which is a complex involved in RNA degradation. Increases the RNA binding and the efficiency of RNA degradation. Confers strong poly(A) specificity to the exosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the archaeal exosome complex. Forms a trimer of Rrp4 and/or Csl4 subunits. The trimer associates with an hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the RRP4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876336.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQE9"/>
    <dbReference type="GeneID" id="76209818"/>
    <dbReference type="OrthoDB" id="35160at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071034">
        <property type="term" value="P:CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22524">
        <property type="entry name" value="KH-I_Rrp4_prokar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05789">
        <property type="entry name" value="S1_Rrp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000127">
        <property type="entry name" value="Exosome complex component RRP40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00623">
        <property type="entry name" value="Exosome_Rrp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026699">
        <property type="entry name" value="Exosome_RNA_bind1/RRP40/RRP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023474">
        <property type="entry name" value="Rrp4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054371">
        <property type="entry name" value="RRP4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048565">
        <property type="entry name" value="S1_RRP4"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003181">
        <property type="entry name" value="PRK04163.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321:SF4">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321">
        <property type="entry name" value="PNAS-3 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22625">
        <property type="entry name" value="ECR1_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15985">
        <property type="entry name" value="KH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110324">
        <property type="entry name" value="Ribosomal L27 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0271">Exosome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="7">
        <location>
            <begin position="61"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="226" mass="24695" checksum="012BE7D9BBD85E8F" modified="2018-07-18" version="1">MDNKRKYVIPGDVVTTGPFRPEQNVELQGNKIISTTIGISEIYDDSVKVIPLTGKYIPKINDLVIGKVISHTSLSWELDINSCYVGFLPAQDVFGRDFSAHADELASKLKSGDLVAARIANFDRTRDPLVTISDRDLGKIDTGILLQISPSKVPRLIGKRGTMIQMIEMATDAAITIGQNGWVVVSCESPEGLLKAKKAIEMVNEKAHVANLTDQVKEMLDSKGES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQG0</accession>
    <name>A0A2S2KQG0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar assembly protein FlaJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_07110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866833.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQG0"/>
    <dbReference type="GeneID" id="76208964"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056569">
        <property type="entry name" value="ArlJ-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402:SF2">
        <property type="entry name" value="FLAGELLA ACCESSORY PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="377"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="456"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="494" mass="54526" checksum="B47A67254F2298C0" modified="2018-07-18" version="1">MMVNKVKDVKNLFTSGSKVDENFVYFIALLYCISTGEIGAVDLFKTGKDSKYGKYSNAFRDTYRLGVGWSYGLASACEMMGRKVSKQKDDPTKLLLVKLSQVVRLGDDLKTFFRDELKNSLASYSIKYEANLETQKLFSEMFYTLMSTASFMISANAIMSMLMGFGDSETILIISMMGTGIGMAVFVAMMFFMFPRDVLAHTDDAIEKQFRMKMYIGIFGTVGISSVLLITNMVSPILAVGLGGIPLLYPGIIAKKQESKISTYTEWYPTFILHFGQLLSTVGSMGQALQAVMRSNFGTIQIFIDGLKNRIKNRIDQKICFQLFSIEAGHHIIANGNDIVSTSIYKGADMNLVGNVVADITKKISELRGKRAQNASTFQLVVVILHVLSLSIFGLMNKLTELFNDLSEGDLSNAAFELSPIDPVLMSMLMPVLLIMTSIISGFAIKIIQGGLYKTVFFHIGLLLVVGGISMFAINEFMADFLDSIIFTAPLPGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQG1</accession>
    <name>A0A2S2KQG1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent DNA helicase Hel308</fullName>
            <ecNumber evidence="10">5.6.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">DNA 3'-5' helicase Hel308</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">hel308</name>
        <name evidence="13" type="ORF">NZNM25_06800</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">DNA-dependent ATPase and 3'-5' DNA helicase that may be involved in repair of stalled replication forks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the helicase family. Hel308 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="10"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876518.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQG1"/>
    <dbReference type="GeneID" id="76210040"/>
    <dbReference type="OrthoDB" id="371946at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003787">
        <property type="entry name" value="ATP-dependent DNA helicase Hel308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00442">
        <property type="entry name" value="Helicase_Hel308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048772">
        <property type="entry name" value="Hel308-like_dom4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022965">
        <property type="entry name" value="Helicase_Hel308"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF10">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE HEL308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21280">
        <property type="entry name" value="Helicase_dom4_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="33"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="238"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH33889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="708" mass="79184" checksum="1F15FA81817AD122" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQH0</accession>
    <name>A0A2S2KQH0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Dicarboxylate/amino acid:cation symporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_06900</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876528.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQH0"/>
    <dbReference type="GeneID" id="76210051"/>
    <dbReference type="OrthoDB" id="3015at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006835">
        <property type="term" value="P:dicarboxylic acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3860.10">
        <property type="entry name" value="Sodium:dicarboxylate symporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001991">
        <property type="entry name" value="Na-dicarboxylate_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036458">
        <property type="entry name" value="Na:dicarbo_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42865">
        <property type="entry name" value="PROTON/GLUTAMATE-ASPARTATE SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42865:SF7">
        <property type="entry name" value="PROTON_GLUTAMATE-ASPARTATE SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00375">
        <property type="entry name" value="SDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00173">
        <property type="entry name" value="EDTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118215">
        <property type="entry name" value="Proton glutamate symport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="97"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="378"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="438" mass="47091" checksum="0965721BCBD89794" modified="2018-07-18" version="1">MVIKSFKYPIPQITYLVKKRLWAQVLFALFLGLVAGIILGPEIGLVEKETAGVITDWLSIPANLFLKIIQMIIIPLIFASIIRGITSSGSMEQLQKLGLGVSVYFVATTIIALTIGILLVSTIEPGNFIDSSLIRESFGIEDAEPVEKAEFSIQDIPQSIVSLIPSNPLASFMSGEMLSIIIFALIVGVSMISIPKQSSLPILDLLESIQKITLKVVSWAMRLAPFAAFGLMAGITSKIGLSALGVLGAYMLTVVIGLFVMILVYMLIIKFFAKRPLSSTFKMMKDAQLLAFSTSSSAAVMPLSIKTAEEKMKIKPKVSQFIIPLGATINMDGTALYQIVAVFFLAQLFSIDLGFTTILLITLTALAASIGAPSAPGTGIVILSTILITAGIPPVAVVLLLGVDRILDMTRTMVNVTGDLTACLVFDKRVKEEDIVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQJ2</accession>
    <name>A0A2S2KQJ2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_07330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_160049200.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQJ2"/>
    <dbReference type="GeneID" id="76208942"/>
    <dbReference type="OrthoDB" id="2857at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17541">
        <property type="entry name" value="REC_CheB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42872">
        <property type="entry name" value="PROTEIN-GLUTAMATE METHYLESTERASE/PROTEIN-GLUTAMINE GLUTAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42872:SF6">
        <property type="entry name" value="PROTEIN-GLUTAMATE METHYLESTERASE_PROTEIN-GLUTAMINE GLUTAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="1">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="145" mass="16227" checksum="89F2CFE8B649EC00" modified="2018-07-18" version="1">MIINDSRAMRLFLEDIVNSYFGWQLIGSYSDATYALSVLENKKPDVILLDLEMPKMDGFTFLERLGNVGIYPTIITSNYAIDGSEIVSDALALGAVDYIVPPSSNSKVDFDKFKLRLRHKITKASLKSSRYSLFSNSGKSKSRIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQJ4</accession>
    <name>A0A2S2KQJ4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_07240</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQJ4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00156">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050595">
        <property type="entry name" value="Bact_response_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591:SF3">
        <property type="entry name" value="RESPONSE REGULATORY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591">
        <property type="entry name" value="STRESS RESPONSE REGULATOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="22"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="146" mass="16845" checksum="01423D0A7F4AA214" modified="2018-07-18" version="1">MFVQKFKTIPFDAEKKKINMTTAVIIDDDFDTVEVFSEFLSLQNIDVLGKAYNGLDGIKLFEEKKPDIVFSDLWMPEYDGFYLLTNLRQKFKEAKIIMVTADLTQETDRKLKECNVDAVIFKPFKISQIMEAVEKVSNKSKPVIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQJ8</accession>
    <name>A0A2S2KQJ8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Signal recognition particle receptor FtsY</fullName>
            <shortName evidence="8">SRP receptor</shortName>
            <ecNumber evidence="8">3.6.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ftsY</name>
        <name evidence="11" type="ORF">NZNM25_05190</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.4"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the GTP-binding SRP family. FtsY subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.4" evidence="8"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876381.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQJ8"/>
    <dbReference type="OrthoDB" id="372188at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005047">
        <property type="term" value="F:signal recognition particle binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000566">
        <property type="entry name" value="Signal recognition particle receptor subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00920">
        <property type="entry name" value="FtsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004390">
        <property type="entry name" value="SR_rcpt_FtsY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00064">
        <property type="entry name" value="ftsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134:SF1">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="10">
        <location>
            <begin position="273"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="305"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="313"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="346"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="408"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="419"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="112"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="194"/>
            <end position="198"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="252"/>
            <end position="255"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH33728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="430" mass="47568" checksum="78C5E8D4B8E35D33" modified="2018-07-18" version="1">MFDKLRNAFSNAAKSLGEKELNEKDIEDVLFELEISLLESDVATEVIESIKSDLKEKLIGSKVDKKEIEKFVKDSLISSISTLFDSAGTYDLFASINEKKKQGKPFLILFVGINGTGKTTSLAKVAHLLQQAKYSVVVAAADTFRAGAIEQLREHTNRLNLKLVAQNYESDPAAVARDAVLYANSHRTDCVLIDTAGRMQTSKNLMEQIAKITKVVNPDMKIFVGDSLAGNDTVNQAREFYEHVKFDASILTKGDADAKGGAALSIVKITSTPILFVGVGQEYPDLKSFDKKTFLETIFGSLGDVEIKKPEPTPEPTPEPTPEPTPEPTPEPTPEPTPEKKISSDDPFDGIEDDDIATYSDLFDVAPPETDDEAFKLGNKIRQWIKNGKPTPSDSKTEQDEEQNETEDAQKHDKEEKPKKKRGMFGFFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQM0</accession>
    <name>A0A2S2KQM0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07470</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876607.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQM0"/>
    <dbReference type="GeneID" id="76208929"/>
    <dbReference type="OrthoDB" id="2553at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="85" mass="10315" checksum="AF388C3EA048340B" modified="2018-07-18" version="1">MGSNPAQINGKHLKELLKKFKEERDRIEEETGVRPQIDDTTQMFMQKILNVWIDEGKEIDEEKFWLEVDYNRQFSHPVEYYEKSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQM7</accession>
    <name>A0A2S2KQM7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">High frequency lysogenization protein HflD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_07500</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866838.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQM7"/>
    <dbReference type="GeneID" id="76208926"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052776">
        <property type="entry name" value="Chloro_ReproSupport/MetalTrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33876:SF4">
        <property type="entry name" value="CHLOROPLAST PROTEIN FOR GROWTH AND FERTILITY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33876">
        <property type="entry name" value="UNNAMED PRODUCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="55"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="173"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="214"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="25925" checksum="C930259F5BD4999F" modified="2018-07-18" version="1">MLTPALVMGLGLAVGLEHAFEPDHVAAVSTQISKSKYKQKSTKSLLRESFTKSSVLGALWGAGHTTTLILIGFLVYALAITIQEQVFSGLEFTVGIMLVFLGLTTILNKKLRLKHKHPHQHQDGSIHFDEHNHDDSDHEHTHRSYFIGLVHGLAGSGSLVVLTAATLDNVGMVLGFIAIFGVGSMIGMALVGSLMGIPLVFANKIKLVQKCFRYVAGGFSLVIGFNIMYQIGVVGHLFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQN8</accession>
    <name>A0A2S2KQN8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_07600</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876620.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQN8"/>
    <dbReference type="GeneID" id="76208916"/>
    <dbReference type="OrthoDB" id="386895at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007757">
        <property type="entry name" value="MT-A70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829:SF7">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05063">
        <property type="entry name" value="MT-A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51143">
        <property type="entry name" value="MT_A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="226" mass="26491" checksum="0F4B3EDCC8E218A7" modified="2018-07-18" version="1">MTQEITEKPTGYSIYKKTVKKRTIRSHLPEEKFYPALPKTKFDIIYADPPWDYNGKLQFDKSSKDKENLDTSKNLFISTSGFKYPTLKTEKMKEIPIQKIAKDDSLLFMWTTNPHLAQSIELGKAWGFEYRTVAFIWDKMVHNPGKYTLSNCELCLVFKHGRIPTPRGARNIRQLIRVPRGKHSEKPKEVMVAIEAMFPHHKRIELFAREKVKGWSNWGLHLDHWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQP0</accession>
    <name>A0A2S2KQP0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ParB-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_07670</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQP0"/>
    <dbReference type="OrthoDB" id="386895at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1530.10">
        <property type="entry name" value="Conserved hypothetical protein from pyrococcus furiosus pfu- 392566-001, ParB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007757">
        <property type="entry name" value="MT-A70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829:SF7">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05063">
        <property type="entry name" value="MT-A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="ParB/Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51143">
        <property type="entry name" value="MT_A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ParB-like N-terminal" evidence="4">
        <location>
            <begin position="4"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="398" mass="45201" checksum="948661755E6F7534" modified="2018-07-18" version="1">MVDLHVNPELFALVPEPTKEDFAALKKSIEANGQHNPIIVWKDPVSSKDFIIDGHSRYETCKQLKIEPIIEYKQFDNWLVAMKFAVQVNSNRRHLSAIQKVELALREIEIEGKIAKERQKSTLPKKGEKGFQKSVPNGMHTGNTCEIVSKKTGLSSRTVARLKRILDDGTDELKRDVASGKKTAASAERLIKLEQIHTNPISLPEGKFKIILCDVPYKYDFEGEGAPNYPTLSEEEIIKLKDQHGRPITDLFATDAIIFFWAPFPKLEDALRIIHAWGFSYKTGIVWSKEKEGISQQGTGHYVVATCETILIATKGKPGIPLPKNRPLGILRAPRTNTHSQKPDITRHWIEKMYPNEKYLELFARETAKGWTAWGNQLDFPKITKPVIKKGILDYLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQR0</accession>
    <name>A0A2S2KQR0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Bifunctional protein FolD</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="12">Methylenetetrahydrofolate dehydrogenase</fullName>
                <ecNumber evidence="12">1.5.1.5</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="12">Methenyltetrahydrofolate cyclohydrolase</fullName>
                <ecNumber evidence="12">3.5.4.9</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="12 15" type="primary">folD</name>
        <name evidence="15" type="ORF">NZNM25_07800</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(6R)-5,10-methenyltetrahydrofolate + H2O = (6R)-10-formyltetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="3.5.4.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methenyltetrahydrofolate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22812"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 12">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.5" evidence="12"/>
    <dbReference type="EC" id="3.5.4.9" evidence="12"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876635.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQR0"/>
    <dbReference type="GeneID" id="76208895"/>
    <dbReference type="OrthoDB" id="9455at2157"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000094">
        <property type="entry name" value="Bifunctional protein FolD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000005">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020867">
        <property type="entry name" value="THF_DH/CycHdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF5">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00767">
        <property type="entry name" value="THF_DHG_CYH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 12" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 12" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7 12" id="KW-0521">NADP</keyword>
    <keyword evidence="3 12" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 12" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="13">
        <location>
            <begin position="6"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding" evidence="14">
        <location>
            <begin position="139"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="165"/>
            <end position="167"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GBH33989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="287" mass="31026" checksum="1090FDB3EEDAE8F7" modified="2018-07-18" version="1">MAGVKIDGKVIAQSVKDRVKKAILELKTQDINPCLATILVGENPASATYVKNKHIACEEVGIITKDHKLASNITQMELNKIIDNLNTDNSVHGILVQLPLPEQLDEFATTSRISPLKDVDGLTPHNAGLLAMKKAALVACTPSGVMEMFDFHKIDLEGKNVVLINRSNLVGKPLYHLLLEKNATVITCHSKTKNITEFCKSADIIITAVGDRNKFTLTSEMIKDGAIVIDVAISRFQEKLVGDSDYDKIIQKASFATPVPGGVGPMTVAMLLKNTITAASLSSQIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQT8</accession>
    <name>A0A2S2KQT8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SAM-dependent methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_06090</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876459.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQT8"/>
    <dbReference type="GeneID" id="76209962"/>
    <dbReference type="OrthoDB" id="6027at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026170">
        <property type="entry name" value="FAM173A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13610">
        <property type="entry name" value="METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13610:SF11">
        <property type="entry name" value="METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="252" mass="29301" checksum="13B29BBB7391032E" modified="2018-07-18" version="1">MKIEEYLETLPENILNGEDVHLPEKSLREIFKFVNLGKNDVFYHLGCGNEKGVKMAINEFNVKKAIGIDNNDEKIQHAKENFGESNCVQFICKNIEDSDISEASVILFWFTDENMINKMMKKFENLKPETKIITIWGPLQDCLPDEVNFPYIINKTPFKRAENLQEQLLSIFGVKCIDFVTAWEFAERYTKSIGMPEIQNDRFLTIIQTLMIWINAKKLGVACGEEIPESIQTYISIMKMHFDIDFEHLLDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR35</accession>
    <name>A0A2S2KR35_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07920</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR35"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="463" mass="51577" checksum="3A81892A60C7F7F5" modified="2018-07-18" version="1">MTIFTIGILGLVGTAFAIPPFSSQEVFDFSDTIVVGKVILINSTFSPTHNLYHIQVEKFLKNPHDSDVVLAAGQNITSSRSGTQIFHVGDRALFFLTAVFGYGQYHNMLSIHPTTKLVESDWDKCNIFENNIPSDHWMLGGTGSLPKISQDETLDINAFQDRNNFKVGKTVTVSYDISNISDRPKEIDLDGILSRSNGAEFEAMSTMSQHVVLEPCTVYKTIEWRFTPGMTGSYLFEINNNSGTSYGLGFAVKESVESPLAQLKPSGVMGHVQTSSTSYVVGDEVFVRIDTKPNSDVVVDVLNPSDKITEHFVAISDEYGQINTSFLLPHDAMQGTWKVKATYGSDFETLEFDVKERLVPEPEQSRQVSENCGPGTTLQDGICVVDETKENHVDSDRWGGLVPWMIESPLKQIKKGIEPWEVKCNDGLIRIFKSWDLHTPACVFPDSIPKLNERGWQTLEDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR75</accession>
    <name>A0A2S2KR75_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_09630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876794.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR75"/>
    <dbReference type="GeneID" id="76208707"/>
    <dbReference type="OrthoDB" id="2830at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052048">
        <property type="entry name" value="ST_Response_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228:SF1">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ARR22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="1">
        <location>
            <begin position="4"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="128" mass="14167" checksum="5936D8AA31CA22CE" modified="2018-07-18" version="1">MPPTVIVIDDSHALTQVVSEFLTLNSIDVLAVGQNGKEAVELYKKHSPDFVLMDYLMPEFNGLYGLQNIRKTNPNAKVIMFTGSADPGLFDQFRESGVFAILEKPCEFDKLVETINNTPSSNTVQLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR88</accession>
    <name>A0A2S2KR88_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">YnfA family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_07490</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876609.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR88"/>
    <dbReference type="GeneID" id="76208927"/>
    <dbReference type="OrthoDB" id="129688at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00010">
        <property type="entry name" value="UPF0060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003844">
        <property type="entry name" value="UPF0060"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002586">
        <property type="entry name" value="PRK02237.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36116">
        <property type="entry name" value="UPF0060 MEMBRANE PROTEIN YNFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36116:SF1">
        <property type="entry name" value="UPF0060 MEMBRANE PROTEIN YNFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02694">
        <property type="entry name" value="UPF0060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="12216" checksum="6113A33F0240D88F" modified="2018-07-18" version="1">MLEITSTIVVFFFAALLEIGGGYLVWKWLRDHKGKILGLTGGLILFSYGIVMTLQPADFGKVYATYGGIFVVSSIIWGYWIDKKIPDRFELIGSVVVLIGVAVMFYFPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR95</accession>
    <name>A0A2S2KR95_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_09640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR95"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="54" mass="6454" checksum="6911912A24A9DF69" modified="2018-07-18" version="1">MFLYKKKHYKLDKMEKICCVCGKEFENRSTDFCSRECACENSEELVLHTMPKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRB3</accession>
    <name>A0A2S2KRB3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Lysine biosynthesis enzyme LysX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_09450</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RimK family. LysX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876779.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRB3"/>
    <dbReference type="GeneID" id="76208725"/>
    <dbReference type="OrthoDB" id="33241at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043774">
        <property type="term" value="F:coenzyme F420-2 alpha-glutamyl ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009085">
        <property type="term" value="P:lysine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000025">
        <property type="entry name" value="Alpha-aminoadipate--LysW ligase LysX protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000058">
        <property type="entry name" value="Alpha-aminoadipate--LysW ligase LysX protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000044">
        <property type="entry name" value="Lysine biosynthesis enzyme LysX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054562">
        <property type="entry name" value="LysX/ArgX_preATP_grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011870">
        <property type="entry name" value="LysX_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004666">
        <property type="entry name" value="Rp_bS6_RimK/Lys_biosynth_LsyX"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02144">
        <property type="entry name" value="LysX_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00768">
        <property type="entry name" value="rimK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621:SF2">
        <property type="entry name" value="COENZYME GAMMA-F420-2:ALPHA-L-GLUTAMATE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 MODIFICATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22626">
        <property type="entry name" value="LysX_preATP_grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="11">
        <location>
            <begin position="93"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH34154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="280" mass="31402" checksum="377F67E6CF7863F2" modified="2018-07-18" version="1">MSPDVTILYDTIRWEEKALLEAGKKNNINIQMVDCKKVALDLEKKPEDYGVVIQRCVSYYRNLHSTAALEGLGVKVINCLNTGIFAGNKLFTHMLLKKFGVPTPDATVAFSKEAALEALETQGFPKVIKPTVGSWGRLISKLNDKDSAEGIMESRENMYPIYQIHYLEEFVKRPPRDIRAIMVGDKIVAAIYRISEHWKTNMALGGTAEPCKVTSEMEEMCIKAKKAVQGDIVGVDLMESEEKGLVVHEVNNTTEYKNTVRVCQVDIPSLMLNYALNVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRH5</accession>
    <name>A0A2S2KRH5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_10440</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876862.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRH5"/>
    <dbReference type="GeneID" id="76208620"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="90" mass="10597" checksum="AD561BFBC1253989" modified="2018-07-18" version="1">MYKVYQVIKKFDSRKDHGFSMRPQYARCLEVMNTGEKINAKEFAKEHRGEFVIRRNTLESNLADTHIAFEPNANTNVLYLNPFQNTKPMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRH9</accession>
    <name>A0A2S2KRH9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_09320</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876766.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRH9"/>
    <dbReference type="GeneID" id="76208738"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052528">
        <property type="entry name" value="Sugar_transport-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23526">
        <property type="entry name" value="INTEGRAL MEMBRANE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23526:SF2">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="345"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="6"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="366" mass="40034" checksum="77EECB398897FCA6" modified="2018-07-18" version="1">MQKLQVNNLVRSATFFQHAGISIIFVFMPIIAKGVTESIFEIGLLVASFSFAQILSEIYFGRHSDKKGTRLKFIRIGFIGCAITFGLHYFAADLTMFFIVRIAAGVASGIMIPAMIAYTYEANIDKKRAATVISFHALGWLAGIAAAGIANDLKLIFLISAASFVIGLLFTIKLPNPQQEKELKPGTTKNVIAKNKFLFSSLLLRHIGAAAVWTILPIMLMERLGAELYQVSIVYVANTLTAFILMNVMASKIHLSNVTKFKIGIGFTTFVFIGLVLVTDWWMVMPFMSLVGATWAFLFIGGNFHLMENNPRSTSTGIFSSTLSIATVVGPVVAGSIAFVFDYAAVMYFAIAIIVCAFVVSLKIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRM2</accession>
    <name>A0A2S2KRM2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Translation initiation factor 2 subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">aIF2-beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">eIF-2-beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_10900</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876941.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRM2"/>
    <dbReference type="GeneID" id="76208569"/>
    <dbReference type="OrthoDB" id="38099at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.170:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003067">
        <property type="entry name" value="PRK03988.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation initiation factor IF2/IF5" evidence="7">
        <location>
            <begin position="25"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="140" mass="16284" checksum="DC6284E5304DDCFE" modified="2018-07-18" version="1">MTKAEYEKLLKRIQDKLGDNNEDSSTRFELPIVDVMWEGQKTFLRNFSEFPKVLRRDPDKVLQYLSKEFAVPAERLGDKAMFVGRRAPDDFTRLFQIYVKDYLECPTCKSPDTKILKENRISFLICEACGAKSTLKGKYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRR7</accession>
    <name>A0A2S2KRR7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876994.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRR7"/>
    <dbReference type="GeneID" id="76208501"/>
    <dbReference type="OrthoDB" id="12384at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="158" mass="18252" checksum="810CF5DB36089F16" modified="2018-07-18" version="1">MTSQYVIIGIAVGVFIAGIGVGYGVLQSTSTPNYMQMTPQQMQQMMNDPNMMNQWHQTMMNDPNAMNQWMGMMINDPQAMTQMHNTMMDNNQHMSTMMQPMMNHMMNDPVMRDQMMGMMMNNQGMMNHMMSNQQMMNMMGMNNSMMMGNMMGNNMMMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRS3</accession>
    <name>A0A2S2KRS3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Putative [LysW]-lysine/[LysW]-ornithine hydrolase</fullName>
            <ecNumber evidence="8">3.5.1.130</ecNumber>
            <ecNumber evidence="8">3.5.1.132</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">lysK</name>
        <name evidence="10" type="ORF">NZNM25_09460</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the release of L-lysine from [LysW]-gamma-L-lysine and the release of L-ornithine from [LysW]-L-ornithine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[amino-group carrier protein]-C-terminal-gamma-(L-lysyl)-L-glutamate + H2O = [amino-group carrier protein]-C-terminal-L-glutamate + L-lysine</text>
            <dbReference type="Rhea" id="RHEA:48684"/>
            <dbReference type="Rhea" id="RHEA-COMP:9693"/>
            <dbReference type="Rhea" id="RHEA-COMP:9715"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:78525"/>
            <dbReference type="ChEBI" id="CHEBI:78526"/>
            <dbReference type="EC" id="3.5.1.130"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[amino-group carrier protein]-C-terminal-gamma-(L-ornithyl)-L-glutamate + H2O = [amino-group carrier protein]-C-terminal-L-glutamate + L-ornithine</text>
            <dbReference type="Rhea" id="RHEA:52676"/>
            <dbReference type="Rhea" id="RHEA-COMP:9693"/>
            <dbReference type="Rhea" id="RHEA-COMP:13328"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:78525"/>
            <dbReference type="ChEBI" id="CHEBI:136763"/>
            <dbReference type="EC" id="3.5.1.132"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="8">Binds 2 Zn(2+) or Co(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-arginine biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (Thermus route): step 5/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase M20A family. LysK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.130" evidence="8"/>
    <dbReference type="EC" id="3.5.1.132" evidence="8"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829067.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRS3"/>
    <dbReference type="GeneID" id="76208724"/>
    <dbReference type="OrthoDB" id="133929at2157"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00039"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01120">
        <property type="entry name" value="LysK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010175">
        <property type="entry name" value="LysK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050072">
        <property type="entry name" value="Peptidase_M20A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01902">
        <property type="entry name" value="dapE-lys-deAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808:SF28">
        <property type="entry name" value="[LYSW]-LYSINE_[LYSW]-ORNITHINE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808">
        <property type="entry name" value="ACETYLORNITHINE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0170">Cobalt</keyword>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="9">
        <location>
            <begin position="159"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="361" mass="39838" checksum="EE4536EEFB6221C0" modified="2018-07-18" version="1">MLEKALRLYTPSLSEKQLAEFLADKCDDLGFEDIEIDEVGNIIAKKGTGSPKVMLCGHMDVVPGKVKVRKEGDSLYGRGASDAKAPLMAMLFAAASIQNNSGTIIFVGAVDEEGNATGIKNLVKKEMGIDYAIFGEPSGINQVTIAYKGRLAISLKISVDDSSHASAPWLSKNAIHESMIFAKELKDRLEENQENRSKGMLLTSSMTEIRGGTSHNITPKECETTFDIRIPVDMNCKAIEQKIATLVKEISQKREVEAFYSILDETEPFEAPHNSPLVRALTLGIIEETKSRPTLIRKTGTGDMNVLGNQWSIPVVTYGPGDPHEAHTIDEKVSIEEYLKGIEILKKTLQHLKRLHDRKMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRS7</accession>
    <name>A0A2S2KRS7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phenylalanine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">NZNM25_11440</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876985.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRS7"/>
    <dbReference type="GeneID" id="76208512"/>
    <dbReference type="OrthoDB" id="372178at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00496">
        <property type="entry name" value="PheRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000095">
        <property type="entry name" value="Phenylalanine--tRNA ligase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003210">
        <property type="entry name" value="PRK04172.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF40">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="12">
        <location>
            <begin position="320"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH34353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="461" mass="52723" checksum="5608A43B0CE6DB20" modified="2018-07-18" version="1">MSQVFHEIEKKIITSLKDNPKQTMEKLEKSTNLSSDQIRRGVEWLKLKELANVVESTTSIFSLGKNGLEAFEKGLPERRLLNLLKDGPKTMQDLQKELGSIFGPAMGLARKNNWIETNEKISLKHSPIELPGEKTLKKIGNGKQPKNEIDVVDLSSLLTRPDFLIEDIQKSKEISLTNHAKSIELSESIGAIDVEAEVPQIFAARTHPLKDTIDEIREIFVTLGFSEIVGSMSQSSFWNFDALFTPQDHPARELQDTFYLDVVSAKKIGTPEQIKNVSASHKKNWRYLWDVNEARKMVLRTHTTCVTIKHLAETKPDEARVFSVGRVFRNEKVSYKHLVEFNQIEGVVVGKNATLRDLMGIQREFYKRIGITKIKFWPTFFPYTEPSLQTMVYNDRLGKWVELFGMGIFRPEVTKPLGINKPVLAWGGGIERIAMLKYGLDDVREFYNNNLNWLRSVNKCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRW2</accession>
    <name>A0A2S2KRW2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11870</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877039.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRW2"/>
    <dbReference type="GeneID" id="76209319"/>
    <dbReference type="OrthoDB" id="7369at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="105" mass="11561" checksum="D4074961E9C2227C" modified="2018-07-18" version="1">MSTKEFDVNGTDYKIVLTEQVIGHVNNLKSLYNAAYEDPESFEDVSSEISSTINEIASTVEPPAADSDLDGLIQEIIKAVESKADEIKKELEAKEKPAKKSKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRZ2</accession>
    <name>A0A2S2KRZ2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CbtB-domain containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_12410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877084.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRZ2"/>
    <dbReference type="GeneID" id="76209263"/>
    <dbReference type="OrthoDB" id="8286at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012667">
        <property type="entry name" value="CbtB_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09489">
        <property type="entry name" value="CbtB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="71" mass="7795" checksum="6FACBC962F52E34A" modified="2018-07-18" version="1">MSETRQISVSKTGVSKLAIVTLAIIFTAGLFVVGFDQGHVFSLVYGDQAFVDLYLHELTHDMRHAAGFPCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRZ7</accession>
    <name>A0A2S2KRZ7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12330</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRZ7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="53" mass="6100" checksum="3FB6D964F9B8761D" modified="2018-07-18" version="1">MAMFIITAVYVGYHAMQQPVEEIKDHNVEFELLNVNSGIEQIDQNTIIIKTHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS05</accession>
    <name>A0A2S2KS05_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Aconitate hydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">acn</name>
        <name evidence="8" type="ORF">NZNM25_11380</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS05"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000002">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000003">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000004">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1060.10:FF:000001">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1060.10">
        <property type="entry name" value="Aconitase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050926">
        <property type="entry name" value="Aconitase/IPM_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015932">
        <property type="entry name" value="Aconitase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006248">
        <property type="entry name" value="Aconitase_mito-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01340">
        <property type="entry name" value="aconitase_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005558">
        <property type="entry name" value="PRK07229.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160">
        <property type="entry name" value="ACONITATE HYDRATASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160:SF4">
        <property type="entry name" value="ACONITATE HYDRATASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="6">
        <location>
            <begin position="39"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="7">
        <location>
            <begin position="563"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="757" mass="83024" checksum="620840660173694E" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS21</accession>
    <name>A0A2S2KS21_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 13">Valine--tRNA ligase</fullName>
            <ecNumber evidence="2 13">6.1.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">NZNM25_11320</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tRNA(Val) + L-valine + ATP = L-valyl-tRNA(Val) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="2 13"/>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876975.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS21"/>
    <dbReference type="GeneID" id="76208525"/>
    <dbReference type="OrthoDB" id="23906at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000192">
        <property type="entry name" value="Valine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000362">
        <property type="entry name" value="Valyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009687">
        <property type="entry name" value="PRK13208.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946:SF93">
        <property type="entry name" value="VALINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="VALYL-TRNA SYNTHETASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="14">
        <location>
            <begin position="18"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="15">
        <location>
            <begin position="607"/>
            <end position="739"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="NCBIfam" id="TIGR00422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GBH34341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="772" mass="89847" checksum="48CFC417120B3FEE" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS45</accession>
    <name>A0A2S2KS45_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877100.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS45"/>
    <dbReference type="GeneID" id="76209242"/>
    <dbReference type="OrthoDB" id="2931at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="119" mass="14058" checksum="66FBAAA2BF09EB1A" modified="2018-07-18" version="1">MSERKPFRRFRKREPSLTTEQKIEQFIIRNSKNGYFTKVSTIPYKFEISEDATWNIIGGLLSSGVLESFHDEVTGEMKLCETGKIYSIMDLEKKRKRTKFHTTKNNKPILKKKKIQKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSB9</accession>
    <name>A0A2S2KSB9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_13300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSB9"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="176" mass="19633" checksum="D7E3A09BB21D1EB8" modified="2018-07-18" version="1">MKNAFSHPFLKNRRAVSQVMGSVVILGIVSSVGSVILFNGMNSISAFTYDLSFHEASKNQIHREDIIFEHVRFEPNTDNLIIDLANIGTVESTIVSITILNIDNQEIISNWVDLDQTIQLKDNRQLVLDLLDDAQIILVQGSGTWNDPAYVNSEYRISLTTAKGNFFTTVASPFNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSC9</accession>
    <name>A0A2S2KSC9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_13400</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSC9"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051258">
        <property type="entry name" value="Diverse_Substrate_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42920:SF5">
        <property type="entry name" value="EAMA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42920">
        <property type="entry name" value="OS03G0707200 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="10"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="134"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="311" mass="34563" checksum="CE738B71DE2A7423" modified="2018-07-18" version="1">MLDMGVNTMEINPIFMTFLIYMICTAFFTPIARKTDAISKFSQKDILFMALIGLSEVAALSTYFFGLQNSSAVNASIFSNSEIIFSLVLAMVIFKERINIKECIPFSMIIIGMMIIPIGNNIYQNGMNFEHLATGDLLIILSGFLYAADITICKYVGDKYDARRVTQITSFFCAIIALSFLFLLQIPMDFKLEYIPNIMLISIFGTGMSTLLFLAGLKLIGAVRTVLLYSTTSVFGIIFAGVFLSEQITIIDLISLGITLIGIFFLRNKLAESESADPELEGSSVPNNGDEFEHKFNENFTKNNPKTVISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSK4</accession>
    <name>A0A2S2KSK4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aldo/keto reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_13220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877147.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSK4"/>
    <dbReference type="GeneID" id="76209176"/>
    <dbReference type="OrthoDB" id="7238at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19099">
        <property type="entry name" value="AKR_unchar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053135">
        <property type="entry name" value="AKR2_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43312">
        <property type="entry name" value="D-THREO-ALDOSE 1-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43312:SF1">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="1">
        <location>
            <begin position="36"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="366" mass="40883" checksum="669D4C14522FB847" modified="2018-07-18" version="1">MISGFATSDGTKKFAQSSGTNQDNFKNFANLTLSNVGIGTYLGNPDSRTDELVKNAVKQSILSGINVVDTAINYRAQKAERSVGKAISELIDEEKISRDQLFLSTKNGYVTNDADVKLGFWEYVKEEYSQKGVIKEGDVTSGYHCMTPTYLSDQLDRSLKNLNMDCIDLMYLHNAVEGQINDVSKEQFLENLKSVFELYEQKRDEGKIKFYGMATWECFRVPHDSPQYLSLEDTVNLAKKIGGEDNGFRFIQLPFNMHYDQALLGKNQLLKSKQVSILEAAASMGIGVFTSVPFMQGRLLTPGVMPEFNELKPSLRALQFIRSSPGVLAPLVGQKSVEHVSENLEIMKIPPIPEDDFLALVKKLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSK9</accession>
    <name>A0A2S2KSK9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Phosphonate ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">phnE</name>
        <name evidence="9" type="ORF">NZNM25_12360</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877318.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSK9"/>
    <dbReference type="GeneID" id="76209268"/>
    <dbReference type="OrthoDB" id="338493at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015416">
        <property type="term" value="F:ABC-type phosphonate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005769">
        <property type="entry name" value="PhnE/PtxC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01097">
        <property type="entry name" value="PhnE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30043:SF1">
        <property type="entry name" value="ABC TRANSPORT SYSTEM PERMEASE PROTEIN P69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30043">
        <property type="entry name" value="PHOSPHONATES TRANSPORT SYSTEM PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="59"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="261" mass="28750" checksum="8FE8484A8FDFAF9F" modified="2018-07-18" version="1">MTPKNNIIIGIIVALLVVASYNVDANPMEFVDGLPNLVIIIDEMLQVESKYIPTAFLAMFETIQMAFIGTVVGVAIALPLSLLAARNLNSKFVYAPTRALLAATRTFPSILWALLFVIMVGLGPFAGVLAIIMYTVGFIAKLQYEVIETIDSDPMDAVSSIGVSKLQLIRYVVIPESASHLLSQILYMFDYNVRQTSILGLVGAGGIGFYIINYIKFFEYGKAAIFMIVVLVTVLIIDWVSVKIRDKYIIKSQHGMEITVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSM5</accession>
    <name>A0A2S2KSM5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_14400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877244.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSM5"/>
    <dbReference type="GeneID" id="76209055"/>
    <dbReference type="OrthoDB" id="36578at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="36"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="65" mass="8007" checksum="EEDA2AF1D5753AB4" modified="2018-07-18" version="1">MGFFKKTKCDLCDKKFSKEEELMNHKQIVHGKDLQYDCKECNKFFSNMEDMRTHLQREHSYKKDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSN6</accession>
    <name>A0A2S2KSN6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_14730</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877272.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSN6"/>
    <dbReference type="GeneID" id="76209022"/>
    <dbReference type="OrthoDB" id="7750at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="81" mass="9145" checksum="55BBF455F92D7EB8" modified="2018-07-18" version="1">MIETQDAKKVVEVIGNNLIGVSHDSNSFAKLPDSFWGYFARGHDKKGTFGVIVTYSEDGKDVDELISMYEKWAEKNKTKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSQ9</accession>
    <name>A0A2S2KSQ9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_14800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSQ9"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13474">
        <property type="entry name" value="SnoaL_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="11"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="136" mass="15350" checksum="5D5AC5EF6631FDFC" modified="2018-07-18" version="1">MIVLSDNEEIINVIETLFQAGITKDLSILKEIHLNDSKFSSFSDLPPYDLKDYQNTIELEELKFVSISDYSYEIKSPKISIFGDTAIVAMGLIQKGMLVDTKAYTGEHMIITGRATFVLVKQPTWKIVHIHLSKIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSY9</accession>
    <name>A0A2S2KSY9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 11 13">1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase</fullName>
            <ecNumber evidence="5 11 13">5.3.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 14" type="primary">hisA</name>
        <name evidence="14" type="ORF">NZNM25_15470</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11 13">
            <text>1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:15469"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="5.3.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11 13">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11 12">Belongs to the HisA/HisF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.16" evidence="5 11 13"/>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877380.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSY9"/>
    <dbReference type="GeneID" id="76208441"/>
    <dbReference type="OrthoDB" id="52866at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00009"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003949">
        <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04732">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000009">
        <property type="entry name" value="1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01014">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023016">
        <property type="entry name" value="HisA/PriA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006063">
        <property type="entry name" value="HisA_bact_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044524">
        <property type="entry name" value="Isoase_HisA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00007">
        <property type="entry name" value="1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090:SF2">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 11 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11 12" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="10 11 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GBH34756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="235" mass="25750" checksum="3B5C126A80F5E2E1" modified="2018-07-18" version="1">MKIIPAIDLMDGQVVRLYKGDPNQKTVYSDDPVDIAKTWEANGADMLHVVDLDATLGLGSNIPIIKKILEQVTIPVEVAGGLRDESLILDVLKICDRVVIGTLAFKDKALLKKLLLSLGSERIVISVDHKDGEIVIHGWQDKTGIKLVDSIKEFLEMGFTEFLLTNVSRDGTLEGPDLEFLEQACNLENANVIASGGISNVNDVKDVKEKNPYGVILGKALYENKISIEEAKRIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSZ7</accession>
    <name>A0A2S2KSZ7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_15630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877393.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSZ7"/>
    <dbReference type="GeneID" id="76208424"/>
    <dbReference type="OrthoDB" id="12077at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="155" mass="16891" checksum="E0680F678061EA4A" modified="2018-07-18" version="1">MLSKRTIIGLVIGSVIIAIGGYSLILHIGTITINENYVVAVGDSTFYTIPAPAHTQQTMTITGDSFDMKLESPGDGLQIPNTSYKKEVSLNWTHLIDGETKIHIQNTGNAELIITGVLVRTSDPIWFTFDLMVITSGMVIIGFSMGFTLRKPKGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT07</accession>
    <name>A0A2S2KT07_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acetolactate synthase small subunit</fullName>
            <shortName evidence="6">AHAS</shortName>
            <shortName evidence="6">ALS</shortName>
            <ecNumber evidence="6">2.2.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Acetohydroxy-acid synthase small subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_14780</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 pyruvate + H(+) = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Dimer of large and small chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the acetolactate synthase small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877277.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT07"/>
    <dbReference type="GeneID" id="76209017"/>
    <dbReference type="OrthoDB" id="85792at2157"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990610">
        <property type="term" value="F:acetolactate synthase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04878">
        <property type="entry name" value="ACT_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000001">
        <property type="entry name" value="Acetolactate synthase, small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="entry name" value="ACT-like. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004789">
        <property type="entry name" value="Acetalactate_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019455">
        <property type="entry name" value="Acetolactate_synth_ssu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039557">
        <property type="entry name" value="AHAS_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054480">
        <property type="entry name" value="AHAS_small-like_ACT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00119">
        <property type="entry name" value="acolac_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008864">
        <property type="entry name" value="PRK11895.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30239">
        <property type="entry name" value="ACETOLACTATE SYNTHASE SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30239:SF0">
        <property type="entry name" value="ACETOLACTATE SYNTHASE SMALL SUBUNIT 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22629">
        <property type="entry name" value="ACT_AHAS_ss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10369">
        <property type="entry name" value="ALS_ss_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="7">
        <location>
            <begin position="4"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="160" mass="17994" checksum="C73F69C7385893AD" modified="2018-07-18" version="1">MWAILSILVENKPGILFKVTHLFRSRNFNIDSISVGVTENPEFSKMTITTYGDEKQVEQIVKQLDKMIDTVKVEHLDEHKTVYRELSIFKIKLSNANDSMEINKLANAYGGKVHDVKRDSIMVELTATPDQIQAFEELAKPFGIIDVARTGVAALQRSGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT32</accession>
    <name>A0A2S2KT32_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-16S rRNA-processing nuclease YqgF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_15950</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829099.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT32"/>
    <dbReference type="GeneID" id="76208391"/>
    <dbReference type="OrthoDB" id="17710at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="21576" checksum="C4E42AF60155FB59" modified="2018-07-18" version="1">MSFDSILPEEILDYSGHLIFTTRKESPKKCKKLLFHEDIFEQPSTVIRGIMMQKLNLDFEEEILILGVDPGQRIGLSVFYYGQEIERSFHLSIEDFVFHIIQILGGLRAKQKIIKIGNGNMVIAKKIVSMLNLTFCSSFELEFVDERKTSLKIKNFNQRGKRDMLSAKYISQRDGFRHSILPLSLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT34</accession>
    <name>A0A2S2KT34_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_15940</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877432.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT34"/>
    <dbReference type="GeneID" id="76208392"/>
    <dbReference type="OrthoDB" id="5605at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="156" mass="17658" checksum="99C3730891F1658C" modified="2018-07-18" version="1">MTCKGICVRYKAQKPVGTGRYASGQRRCQICEIFIKWEGLWCPCCGYRLRTKPRNLKYKAKLRARVEADSIEAKAVADTQPEEIEVEVKPKTKSKSKTVKAKAAKSKVATKTKEKTPCKYCEKLFVYADKHENNCKKNPDVEVVSAHENESIAIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT51</accession>
    <name>A0A2S2KT51_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_14290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877236.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT51"/>
    <dbReference type="GeneID" id="76209066"/>
    <dbReference type="OrthoDB" id="10397at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="92" mass="9929" checksum="0CC73B64BE437CFA" modified="2018-07-18" version="1">MELSSGRKASLVFVGAFVAAGIVLSTMVFPFWNLIREDVFEEVVILTNDNGVCYVETSDNIPKTIENCTLDSGDKATIKFGNGLAWATIVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT54</accession>
    <name>A0A2S2KT54_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_16140</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877450.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT54"/>
    <dbReference type="GeneID" id="76208372"/>
    <dbReference type="OrthoDB" id="115601at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF8">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="106"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="234" mass="26989" checksum="DBF55C2552F7CD44" modified="2018-07-18" version="1">MIDLLDPTNVITRMFSAGKYEEMYNYCKNLLEKIPNDMIALQNIALSLIHLKKYEEAIMYCDKVLEIKNSDTYALKNKIYALENLKEYERVLELCKLILSTNPNDIWALNSMGLSLNELNRHPDALECYDASLRIDPNDVTALMNKAISLSHLQNYKDAIEFYDKAQSIDSTLKEIPLAKSRLFEKLGMDDDAFLAAQGVLNKDMQKIKNDAKENGFSVFHQFCENEFQDYNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTA5</accession>
    <name>A0A2S2KTA5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">A-type ATP synthase subunit D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">atpD</name>
        <name evidence="5" type="ORF">NZNM25_16570</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the V-ATPase D subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877482.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTA5"/>
    <dbReference type="GeneID" id="76208328"/>
    <dbReference type="OrthoDB" id="117390at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00271">
        <property type="entry name" value="ATP_synth_D_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002699">
        <property type="entry name" value="V_ATPase_D"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00309">
        <property type="entry name" value="V_ATPase_subD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11671">
        <property type="entry name" value="V-TYPE ATP SYNTHASE SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01813">
        <property type="entry name" value="ATP-synt_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="3 4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="210" mass="23859" checksum="0EA879E80F183E03" modified="2018-07-18" version="1">MSFGQNVAATKIELFKYKKSTQIAVMVQQILDDKRKVLLKNIEEMIQEASKARGGIWEPLQDIYKSVNEAYLALGTNTVDSVAESTPPVMEVELHVRRVVDVKIPALTVTEKDTKSMPYGFADTNSSIDRASKQIKELMPKICKAAEYENSIFSLAKALEKTQKLLNALENVIVPQYKENIRFILSTLEEREREEFAKLKKVKEKMESGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTD0</accession>
    <name>A0A2S2KTD0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_16930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877655.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTD0"/>
    <dbReference type="GeneID" id="76208293"/>
    <dbReference type="OrthoDB" id="3100at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="160" mass="18774" checksum="2A96FB082D1D855A" modified="2018-07-18" version="1">MVKNNKKKEEKPKKNDFKSFLRKRAPIYLVIITIFVIFAIPELTKSDLQSRFPELTSEQKQVVDILMNYNGPNESGLTVMEAITNRINEEYPDEKIFDNKKTIVELAVSKINDEEYHFILDFESYKGELNYDWNVNKISGKITSNNQDSKHIIDLVDFYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTD7</accession>
    <name>A0A2S2KTD7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_16320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTD7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="275" mass="30252" checksum="EA1C6BE6D83C5375" modified="2018-07-18" version="1">MILPILCAIALLFTPSTVYAHAGHNHGGGGCSDCNPPTLGVDASGKRQVSGGLTINGQTFDAEAFKQDLDSQILQIGEPVEITLKIYDNRGYNELKHVELNLGYEEKLVSGVMIPHHPVTIEWSKTFDGKTTTTVFDKQKLLKDVNVQVLDNSPIIGVKFNFTPVQKFDANTIVTKIWDQKRNANTNYFHNALDIISNKPAPVLANTLNSIQAKISEETTNEPTISDSPIDKVNQDVKCSTKHVQLLRVSDNSPICVDEYQASVLIQNNWAIPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTE8</accession>
    <name>A0A2S2KTE8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_17150</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877531.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTE8"/>
    <dbReference type="GeneID" id="76208270"/>
    <dbReference type="OrthoDB" id="1616at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="150" mass="16856" checksum="E479282EABD10FD7" modified="2018-07-18" version="1">MKSSKKGGLFVLFVLGMSIFGYSQYASASQIGVSVSQSELLHQNDETSNYNIELEFKNPSLLIMTAGETEFFVISNEEMIGKGKLESFTLYPLDSSHVKGTFHTDSNDNAESESVKITGITKYDLWITSIDVPFVYYPTEEQAREFIHQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTF5</accession>
    <name>A0A2S2KTF5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Blue (type 1) copper domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_15360</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877371.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTF5"/>
    <dbReference type="GeneID" id="76208452"/>
    <dbReference type="OrthoDB" id="11836at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000923">
        <property type="entry name" value="BlueCu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052721">
        <property type="entry name" value="ET_Amicyanin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507:SF1">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00127">
        <property type="entry name" value="Copper-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Blue (type 1) copper" evidence="4">
        <location>
            <begin position="72"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="152" mass="16714" checksum="DD11BDF3851D23E4" modified="2018-07-18" version="1">MSGPSSHAYGIGVIALIIGMSASVVFYTSFYLPESLAKPSVDEHILHPEKGLLIEIVPGAVIEGNENYVPNKAEVLLTVDNLVTWQNNDDTAHTVTPDHRHSDSYSGDFGSSGVLKPGDTYEFLFTEPQEVHYHCQPHPWMTGSLTVEISRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTF7</accession>
    <name>A0A2S2KTF7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Flavodoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_17070</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the SsuE family. Isf subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877525.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTF7"/>
    <dbReference type="GeneID" id="76208277"/>
    <dbReference type="OrthoDB" id="9059at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051814">
        <property type="entry name" value="NAD(P)H-dep_FMN_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43408">
        <property type="entry name" value="FMN REDUCTASE (NADPH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43408:SF2">
        <property type="entry name" value="FMN REDUCTASE (NADPH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADPH-dependent FMN reductase-like" evidence="5">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="174" mass="19490" checksum="A4E3AD9BB4483A08" modified="2018-07-18" version="1">MKVVVISGSPRKNANTQIVMKYVYDYAKSKNQETKLINLSEGQIECYRGPDEEYNEATKTAANDIMDADVWLIGSPIYNSFFSSSLKNLFEYINYKKTAGKVAGITILASGSIGFIDVQTLITQLLSYFRVITNPKAVFLTTDSVKENNMLDTDAQNRLKEMVDETLAMGSKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTF8</accession>
    <name>A0A2S2KTF8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7482 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_17250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTF8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055905">
        <property type="entry name" value="DUF7482"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24298">
        <property type="entry name" value="DUF7482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7482" evidence="1">
        <location>
            <begin position="190"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="488" mass="53267" checksum="2E0DF53C08F963B0" modified="2018-07-18" version="1">MLNKLIVFLVIGIFTIGIISISTISDQFANAGSTKKVHFTQTITSSQDPGQGHENHQLALILSPNEGTLYDGSMTFTSSDPVQIVVLHEITSQDAKGQPTWTVDGKTVYGLSLIDLKEKSGSFEFTGAALALHSPNSKEFTSTVSVDGWIRGQPTEVIMQKIELEKEDPSLFLSRANVPATIPMHKGIYNGNQVLYIITDGSDEDFAKTISGKQGWNVEVASAISNIPENTLQKIFIFKNGVKGDGIYGFQNEVFSSTPSQESQYSALNSVIEVTWKKGQKETIFESATDIITAQENGRIEFDETGIVLNTPQITWPDGTMKVRSDKEIFDDMQYGGGQIIEINKEEMTVTFVAHRGWGPDGRTIYYIVTDATPLGPAETMGVVSSPTSANLIAHSGAVDLFQFKNGIKGSGPLGYQAGIAGAALDDKNYSPMWRIYLVEWNDPESAKILETKADIDSFRADGLLSVSIARPLNSDHIVNCPFIDPFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTL6</accession>
    <name>A0A2S2KTL6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_17120</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829108.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTL6"/>
    <dbReference type="GeneID" id="76208272"/>
    <dbReference type="OrthoDB" id="8919at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="51" mass="5916" checksum="51DE969B19E279A8" modified="2018-07-18" version="1">MEKKREIPIEIDDHFKLFGKEPWEVDYGEKCPVCEVRIDEYGFCSCGSSGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTP3</accession>
    <name>A0A2S2KTP3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phosphomevalonate dehydratase</fullName>
            <ecNumber evidence="5">4.2.1.182</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_18310</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of a hydro-lyase that catalyzes the dehydration of mevalonate 5-phosphate (MVA5P) to form trans-anhydromevalonate 5-phosphate (tAHMP). Involved in the archaeal mevalonate (MVA) pathway, which provides fundamental precursors for isoprenoid biosynthesis, such as isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(R)-5-phosphomevalonate = (2E)-3-methyl-5-phosphooxypent-2-enoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:78975"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:229665"/>
            <dbReference type="EC" id="4.2.1.182"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:78976"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterodimer composed of a large subunit (PMDh-L) and a small subunit (PMDh-S).</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.182" evidence="5"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877630.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTP3"/>
    <dbReference type="GeneID" id="76210290"/>
    <dbReference type="OrthoDB" id="18062at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01356">
        <property type="entry name" value="AcnX_swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.10">
        <property type="entry name" value="Phosphohistidine domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012016">
        <property type="entry name" value="PMDh-S-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002840">
        <property type="entry name" value="PMDh-S-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36577">
        <property type="entry name" value="DUF521 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G00490)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36577:SF3">
        <property type="entry name" value="DUF521 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G00490)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01989">
        <property type="entry name" value="AcnX_swivel_put"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004966">
        <property type="entry name" value="UCP004966"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphomevalonate dehydratase small subunit-like" evidence="6">
        <location>
            <begin position="20"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00045120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00045299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00046520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH35040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="128" mass="13548" checksum="C1DA13447BFC0424" modified="2018-07-18" version="1">MKVLVAGKVEGTVLKSENPINFLGTVDKKTGIISDNNHDLFGKSIKDAILVFPSGVGSSVGAYTIYSIKSNKSAPLAMICKKADLTVATGCALANILLITISDKEFDSIKDGMRISLDTDSSNPIQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTQ9</accession>
    <name>A0A2S2KTQ9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_18070</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877609.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTQ9"/>
    <dbReference type="GeneID" id="76210314"/>
    <dbReference type="OrthoDB" id="11042at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="58"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="154" mass="17603" checksum="215407366AFDFBAF" modified="2018-07-18" version="1">MFDKFKNEGGEMVKENVNSEESVEADSTIESTSEIGIGELMGKRAKLEEAIDYVGLMIKNLKDKRTLLEKDIEEESVDIKNLKEKLQKVSEYIDEENRGIHELTKKRQQVENEADEVGSIINTLREKLSGVDKVIDDEGNRVKRIKESRESLTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTS1</accession>
    <name>A0A2S2KTS1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_18330</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877632.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTS1"/>
    <dbReference type="GeneID" id="76210288"/>
    <dbReference type="OrthoDB" id="11330at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="158" mass="17572" checksum="2DDA94595B1C4B25" modified="2018-07-18" version="1">MAKFKKKKSEPTSGPEDSKKESSENPVDIPETEKSEPTSENPSSEPPVSVAEKSEKDRKLNKLFWMRVALAVIAGTAATFIFEDIEGEDRRWASIAFMIMVFFGTIIVAKGMKMQLPSSDRKKIVTQAIGSYVFLYLFTWIVTYTLVHAGSVSSGINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTS7</accession>
    <name>A0A2S2KTS7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Thiol reductase thioredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_18450</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877670.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTS7"/>
    <dbReference type="GeneID" id="76210070"/>
    <dbReference type="OrthoDB" id="35385at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000001">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01068">
        <property type="entry name" value="thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46115">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="18"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH35054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="133" mass="15039" checksum="D4B98A21FB189603" modified="2018-07-18" version="1">MSEDPEIQKIMQKKLAEMLNQKNQPKIEPGIIDLNGSNFDQIISAENPTLVDFWAEWCGPCKSMHPVFESLSKKYPNIKFARVNVDQNQNISIRYGVQSIPTFIMFRSGKVVDKMMGAVGAPGIHMICKKHSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTS9</accession>
    <name>A0A2S2KTS9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sulfate adenylyltransferase</fullName>
            <ecNumber evidence="7">2.7.7.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ATP-sulfurylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Sulfate adenylate transferase</fullName>
            <shortName evidence="7">SAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">sat</name>
        <name evidence="10" type="ORF">NZNM25_18270</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>sulfate + ATP + H(+) = adenosine 5'-phosphosulfate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18133"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="EC" id="2.7.7.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the sulfate adenylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.4" evidence="7"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877626.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTS9"/>
    <dbReference type="GeneID" id="76210294"/>
    <dbReference type="OrthoDB" id="6358at2157"/>
    <dbReference type="UniPathway" id="UPA00140">
        <property type="reaction ID" value="UER00204"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004781">
        <property type="term" value="F:sulfate adenylyltransferase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070814">
        <property type="term" value="P:hydrogen sulfide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00517">
        <property type="entry name" value="ATPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.10">
        <property type="entry name" value="Sulfate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00066">
        <property type="entry name" value="Sulf_adenylyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025980">
        <property type="entry name" value="ATP-Sase_PUA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020792">
        <property type="entry name" value="SO4_adenylyltransferase_pro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024951">
        <property type="entry name" value="Sulfurylase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002650">
        <property type="entry name" value="Sulphate_adenylyltransferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003166">
        <property type="entry name" value="PRK04149.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00339">
        <property type="entry name" value="sopT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43509">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43509:SF1">
        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01747">
        <property type="entry name" value="ATP-sulfurylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14306">
        <property type="entry name" value="PUA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 7 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-sulfurylase PUA-like" evidence="9">
        <location>
            <begin position="7"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Sulphate adenylyltransferase catalytic" evidence="8">
        <location>
            <begin position="166"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH35036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="380" mass="42412" checksum="4184BEDAA8B393F2" modified="2018-07-18" version="1">MSEGNSIKPHGGILVNRIAKVDPTGLFSITITEDLANDVENIADGIFSPLEGFLGKQDFESVVSRGRLANDLAWTIPIVLDVDEQTASKMKESGDVLLKNPDGVGVAVLHVDETYSLDKEKFVQGVYGTNDNSHPGVAKTMSMQDYLVGGKIDYIQRPSDTEIRENRLTPKQTREAFAKAGWKTICAFQTRNPPHVAHEMLQKTSITTRDGVFVNPIIGKKKSGDFVDEVIVKCYETMIKLYYPENRCILGTLHTEMKYAGPKEAIHHAIMRQNYGCTHIIIGRDHAGVGTFYDPFAAQKIFSDYPELEIAPVFFPPFFYCRKCLTYTTPKACPHGDDDKEQISGTKLREMIQNGQAPSEFILRPEVAKVILDHPKPFVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU19</accession>
    <name>A0A2S2KU19_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Thioredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">trx</name>
        <name evidence="8" type="ORF">NZNM25_18720</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008297771.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU19"/>
    <dbReference type="GeneID" id="40721620"/>
    <dbReference type="OrthoDB" id="35385at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000001">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01068">
        <property type="entry name" value="thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45663">
        <property type="entry name" value="GEO12009P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45663:SF11">
        <property type="entry name" value="GEO12009P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000077">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4 6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin" evidence="7">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="site" description="Deprotonates C-terminal active site Cys" evidence="5">
        <location>
            <position position="26"/>
        </location>
    </feature>
    <feature type="site" description="Contributes to redox potential value" evidence="5">
        <location>
            <position position="33"/>
        </location>
    </feature>
    <feature type="site" description="Contributes to redox potential value" evidence="5">
        <location>
            <position position="34"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="6">
        <location>
            <begin position="32"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH35081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="108" mass="11971" checksum="109B903C5C86B9C6" modified="2018-07-18" version="1">MGITQISDAKSWEVDVINSDIPVFVDFWAEWCGPCRMVGPVVEELANDYDGKVKFVKVNVDEANELASKYNVFSIPTLILLNKGEIVSQQVGAASKESYKNMIDRALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU67</accession>
    <name>A0A2S2KU67_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_19730</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU67"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF133">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="273" mass="30292" checksum="04B1F11858FE5749" modified="2018-07-18" version="1">MKENLSQKLFDLSNKVIVLTGSAGRLGSNFAHILGDAGANLILVDIDYKKNEKIEKIISKKFGIKTICSNADITKKAELEELCKLSVKKFGRIDGLVNNAFYSPRTNIKNSAMKFEEYQSDLWNDVVSVNLTGVFLCCQIFGKIMSKQKTQSSIVNISSIYGINGADQRIYGKSKLNSPPSYAATKGAIVNFTRYLAAYWNKKNIRVNTMTLGGVLDESYMDKKFIKNYSEKTIIGRMANSDEYNGALLFLLSNASSYMTGANLVLDGGWSAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU72</accession>
    <name>A0A2S2KU72_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 8">Glutamyl-tRNA(Gln) amidotransferase subunit D</fullName>
            <shortName evidence="5">Glu-ADT subunit D</shortName>
            <ecNumber evidence="5 8">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">gatD</name>
        <name evidence="12" type="ORF">NZNM25_19950</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 8">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 8">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5 8">Heterodimer of GatD and GatE.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 8">Belongs to the asparaginase 1 family. GatD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="5 8"/>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877801.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU72"/>
    <dbReference type="GeneID" id="76210226"/>
    <dbReference type="OrthoDB" id="371959at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08962">
        <property type="entry name" value="GatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="entry name" value="L-asparaginase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00586">
        <property type="entry name" value="GatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006033">
        <property type="entry name" value="AsnA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020827">
        <property type="entry name" value="Asparaginase/glutaminase_AS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027475">
        <property type="entry name" value="Asparaginase/glutaminase_AS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011878">
        <property type="entry name" value="GatD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040918">
        <property type="entry name" value="GatD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037222">
        <property type="entry name" value="GatD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00519">
        <property type="entry name" value="asnASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02153">
        <property type="entry name" value="gatD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003217">
        <property type="entry name" value="PRK04183.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707:SF28">
        <property type="entry name" value="60 KDA LYSOPHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18195">
        <property type="entry name" value="GatD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500175">
        <property type="entry name" value="Glu_ADT_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141300">
        <property type="entry name" value="GatD N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00144">
        <property type="entry name" value="ASN_GLN_ASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00917">
        <property type="entry name" value="ASN_GLN_ASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 5 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 5 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GatD N-terminal" evidence="11">
        <location>
            <begin position="20"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="L-asparaginase N-terminal" evidence="9">
        <location>
            <begin position="94"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase/glutaminase C-terminal" evidence="10">
        <location>
            <begin position="306"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 7">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH35204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="429" mass="47575" checksum="62DCC9CB317E8334" modified="2018-07-18" version="1">MSEYRGYEGNSLEFLKTNQIAVGDSVKITADITYSGIIMPRYEHSDDKHIVLKLKNGYNIGLEIANIEKIEKNQVTEKIIEKAESVEKTEGLPKILLLSTGGTIASKIDYRTGSVTPVLTATELNASVPELAKIANIDAEVLFSEYSENIMPEHWLKIAEKINEYSESDYSGIIIAHGTDTMHYTSSFLSFALAGFSIPIVLVGSQRSSDRASSDAALNLIGATKFITTSNSKGVYIVMHHDENDETIACHIGTRVRKNHTSKRGAFQTIGDVPAFIIAENQIQKNIKEDFFKTNEFQPRINLDTKVALVKYHPGYDSDLLEKIIEMGYKGIIFEGTGLGHIGRTMYDSVKKANDKGIFLGMTSQCIYGRVRMTVYESGRDLLNLGIIPLENMIPEVALVKAMWALGNSQNIEQVKKTMLENIASEISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU75</accession>
    <name>A0A2S2KU75_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_20110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU75"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051207">
        <property type="entry name" value="ComplexI_NDUFA9_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126:SF11">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 39 KDA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="2">
        <location>
            <begin position="11"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="293" mass="33516" checksum="EBC40061A66B19CD" modified="2018-07-18" version="1">MKDIMNKLRKIVVTGATGFVSRNLRKYLSEQNIELVSISRKNFNQFKNETKIISENYEEKKLLKKIKNSDALVHLIGIGKQSVNIDYERTNVEFTSHMVNLSKKAKIKKIVYLSGLGVSATTSLGYFISKYQAEKIIIDSGINYTIFRPSFIVGKDDLLTKSLHKQIKKGLIKIPGSGNYKIQPIHINDVVKIIFKSITETKYRNRIIDLVGPDNISFEEYVRLFSKRTNTRIKKINLEDSYHDAITNPKSDFGIDDLNILIGNFRGNHNKLKKLTGINFESVVELLESSRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU87</accession>
    <name>A0A2S2KU87_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">50S ribosomal protein L2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_19930</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_264953694.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU87"/>
    <dbReference type="GeneID" id="76210224"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.950.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="entry name" value="Ribosomal protein L2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_uL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023672">
        <property type="entry name" value="Ribosomal_uL2_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_uL2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_uL2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Ribosomal_uL2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007180">
        <property type="entry name" value="PRK09612.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="RIBOSOMAL PROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002158">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL2 RNA-binding" evidence="9">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL2 C-terminal" evidence="8">
        <location>
            <begin position="76"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="188"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="220"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH35202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="230" mass="24575" checksum="9C47330CDDD15332" modified="2018-07-18" version="1">MQFRSTSTGKVGSKANYPRFPLAEQHEGKIIDLVHERGREAPLAKIRFEDGSVSYVPAILGARVGETLQFGLKSKIEKGNVISVQNIPDGTIVCNIEKHFGDGGAIVKSAGTNATIFSHGDEGVTVKLPSGRFTTLNPKNRAMIGTLAGGGASERHFMSAGNKWRSFKAKGKKYPIVRGVAQAAYVHPHGGGRHQHVGQSSTVSRDAPPGAKVGSIAARKTGRARIKERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU91</accession>
    <name>A0A2S2KU91_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable endonuclease 4</fullName>
            <ecNumber evidence="7">3.1.21.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Endodeoxyribonuclease IV</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Endonuclease IV</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">nfo</name>
        <name evidence="9" type="ORF">NZNM25_20310</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic (AP) sites, generating a 3'-hydroxyl group and a 5'-terminal sugar phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.</text>
            <dbReference type="EC" id="3.1.21.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 3 Zn(2+) ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the AP endonuclease 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.2" evidence="7"/>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877851.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU91"/>
    <dbReference type="GeneID" id="76210266"/>
    <dbReference type="OrthoDB" id="33250at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008833">
        <property type="term" value="F:deoxyribonuclease IV (phage-T4-induced) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00019">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.150:FF:000001">
        <property type="entry name" value="Probable endonuclease 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00152">
        <property type="entry name" value="Nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018246">
        <property type="entry name" value="AP_endonuc_F2_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00587">
        <property type="entry name" value="nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445:SF0">
        <property type="entry name" value="APURINIC-APYRIMIDINIC ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445">
        <property type="entry name" value="ENDONUCLEASE IV ENDODEOXYRIBONUCLEASE IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00731">
        <property type="entry name" value="AP_NUCLEASE_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51432">
        <property type="entry name" value="AP_NUCLEASE_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Xylose isomerase-like TIM barrel" evidence="8">
        <location>
            <begin position="18"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH35240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="279" mass="30991" checksum="3B8AFA17F1B42563" modified="2018-07-18" version="1">MQIGCHVSISGSIDKAVDNAVERECTAFQIFTRNPRGWHAKELSKEDITNFKTKLKASKIDRFATCAHMPYLPNLATPKDDGFEKSISTLINEVERCAQLGIPYLVTHLGSHLGTGEEAGIKRLVEGLTKAGKTKNDVIILLENTAGQKNSVGSDFKQLGEIFRQLKPAKKFGICIDSCHAFVSGYDLRTEDKVKETFDEFDKYVGLENLKILHLNDAKGELGCNLDRHYHLGMGGIGEKGISALVKFANKKKIPIILETPIDDERDDFENIRIAKELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUE9</accession>
    <name>A0A2S2KUE9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cobalt-precorrin-5B C(1)-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.195</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Cobalt-precorrin-6A synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">cbiD</name>
        <name evidence="6" type="ORF">NZNM25_20120</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the methylation of C-1 in cobalt-precorrin-5B to form cobalt-precorrin-6A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Co-precorrin-5B + S-adenosyl-L-methionine = Co-precorrin-6A + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:26285"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:60063"/>
            <dbReference type="ChEBI" id="CHEBI:60064"/>
            <dbReference type="EC" id="2.1.1.195"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 6/10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CbiD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.195" evidence="5"/>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUE9"/>
    <dbReference type="OrthoDB" id="10423at2157"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00227"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043780">
        <property type="term" value="F:cobalt-precorrin-5B C1-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019251">
        <property type="term" value="P:anaerobic cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2110.10">
        <property type="entry name" value="CbiD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00787">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002748">
        <property type="entry name" value="CbiD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036074">
        <property type="entry name" value="CbiD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00312">
        <property type="entry name" value="cbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000849">
        <property type="entry name" value="PRK00075.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35863">
        <property type="entry name" value="COBALT-PRECORRIN-5B C(1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35863:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-5B C(1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01888">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026782">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111342">
        <property type="entry name" value="CbiD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="2 5 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH35221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="362" mass="39236" checksum="94B564A5EE8C6E98" modified="2018-07-18" version="1">MEEDKTKLKTGYTTGSCATAAAKAALLSIIAQKKIESIEILLPKRSFIQIPVHSCEFGSDKAKCSVIKDGGDDPDVTHGAEIIVELSLTDKINQLEIDGGEGVGIVTKPGLGLEINKAAINPVPKKMINENLREIGEKILVQKGIKVIISVPKGRELGPKTDNPRLGIINGISILGTSGIVIPFSTASYAASIRQNLDVAIAMGNDTVVLTTGGRSEDFAKKIVNLPDHCFVQMGDFSGYTIQQCAKKNIKKAYVVGFIGKLAKMAAGVKQTHVKGSKVDMMFLSKLAEKANANENVIESIKKANTARHVSEIIMENRVDGFFELICTETYKHMRNHSEQKVPIDVILFDFDGNILARKSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUG8</accession>
    <name>A0A2S2KUG8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glycine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Diadenosine tetraphosphate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_20320</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="1"/>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877853.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUG8"/>
    <dbReference type="GeneID" id="76210267"/>
    <dbReference type="OrthoDB" id="6113at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006426">
        <property type="term" value="P:glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.230:FF:000005">
        <property type="entry name" value="Glycine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000002">
        <property type="entry name" value="Glycine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027031">
        <property type="entry name" value="Gly-tRNA_synthase/POLG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002315">
        <property type="entry name" value="tRNA-synt_gly"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00389">
        <property type="entry name" value="glyS_dimeric"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003211">
        <property type="entry name" value="PRK04173.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745:SF0">
        <property type="entry name" value="GLYCINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745">
        <property type="entry name" value="GLYCYL-TRNA SYNTHETASE/DNA POLYMERASE SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01043">
        <property type="entry name" value="TRNASYNTHGLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="9">
        <location>
            <begin position="5"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH35241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="485" mass="55617" checksum="EA10A44B33FE2A1A" modified="2018-07-18" version="1">MNYEEVMKLALERGFYFPSCEVYADAQAGFWEYGPSGVGLKNKFLELWRRELIRRDGMLEIDGSQIMSKSVFEASGHLGNFADPIIKCTKCNSTFRADRTIADISQIEIPESADLEEFDNAIIQNNIKCPKCKGDFEKTKKFNMMFKVGIGPQEEEAYLRPETCQSIFVDFPRLFKTMRGKLPLGIAQVGKSFRNEIAPRQSLLRLREFYQAEIEVFCNPSKLEEVENFAEIENTVIRVQTDAEPVSMTCKEAVESGIIPNKFVAYYLGLLTEFYEKTGIDIAKSRFRKLGDKEKAFYAEVAFDFEVETTIGWLELVACNYRSDYDLSSHATKSKEKFEVMDNDDKVLPHVFEISMGIDRSLYTILEHSLKEDKEHERVVLSIKPYLAPIHVGILSLVKKDGLKEKTDEIYLQIKRKCDAFLDHSGAIGRRYRRLDEIGAPFAVTIDHQTLEDETVTIRKRDSMEQSRIKISELESIAVESIAFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUI7</accession>
    <name>A0A2S2KUI7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sulfatase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_19360</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877748.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUI7"/>
    <dbReference type="GeneID" id="76210166"/>
    <dbReference type="OrthoDB" id="3164at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004065">
        <property type="term" value="F:arylsulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050738">
        <property type="entry name" value="Sulfatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693:SF33">
        <property type="entry name" value="ARYLSULFATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693">
        <property type="entry name" value="ARYLSULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfatase N-terminal" evidence="2">
        <location>
            <begin position="4"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="374" mass="43670" checksum="B496414BBB1DD901" modified="2018-07-18" version="1">MNRKNVIIIMIDGGRLDKAQNSTVFQNLQSKSIFFKNSITYGPHTIAAMHAVFSGSYGSRTGTNSYWSTYKFKKNKFKTLTEYLKDEKYYTHADVINQLVVPKQGFDNYVIHDELNDNLIERHKNLLDEITSNSNCENFFLYLHYSKIHTGIMNEVLKIYDNFSEDFFLNKEKNNFRYDSLFNDAQIYLNEILEKIFLLDLDHNSIILVMSDHGISIGEKVGERAYGAFCYDYTLKTFTYFLIPNYNSKEINQQIRTIDFMPTILDYLEIPLDDQYSKLDGVSLLPLINGASYPELYAFSETGNPLHEKAPPKEPNVKSIRNSKWKLIFNEFDGTKELYDLEHDPDENNNLIGEENDIEEILWNELEKIIKTKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUN6</accession>
    <name>A0A2S2KUN6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NH(3)-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="8">6.3.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">nadE</name>
        <name evidence="10" type="ORF">NZNM25_19490</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>deamido-NAD(+) + NH4(+) + ATP = AMP + diphosphate + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21188"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NAD synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.5" evidence="8"/>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUN6"/>
    <dbReference type="UniPathway" id="UPA00253"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003921">
        <property type="term" value="F:GMP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008795">
        <property type="term" value="F:NAD+ synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025777">
        <property type="entry name" value="GMPS_ATP_PPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002048">
        <property type="entry name" value="PRK00876.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090:SF9">
        <property type="entry name" value="GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51553">
        <property type="entry name" value="GMPS_ATP_PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 7" id="KW-0520">NAD</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMPS ATP-PPase" evidence="9">
        <location>
            <begin position="16"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="47"/>
            <end position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH35158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="329" mass="37700" checksum="9146C025C9320E7A" modified="2018-07-18" version="1">MNNNQMELNEEFLKIKDLEKTTDKICEFIKDEISNKFQKKGAIVGLSGGVDSAVTMALCAKSLGYEQILGLIMPEKESSSSSQLLAKEVAKKFNVETKIIDITQILESFGVYDIKEQIVKEKFPKFNIECKYRVIVPPKSKNIVGIPFLEILDENNERHKLKISSSDFLTLTAATSIKHRVRMTMLYFHAEKNNFSVVGTTNKSEYQQGYFVKYGDGGSDIEPLVNLYKSQIYQLGEFLKVPNQILSKEASPDVWSFSTTDEEFFYSVPYQIVDLILYARENKLSIKDIQKISDLSKEDIESLVKIQNIKQVKSQHMREIPHSWMPDFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUT4</accession>
    <name>A0A2S2KUT4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chaperone protein DnaK</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">HSP70</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Heat shock 70 kDa protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Heat shock protein 70</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 9" type="primary">dnaK</name>
        <name evidence="9" type="ORF">NZNM25_19990</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Acts as a chaperone.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877804.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUT4"/>
    <dbReference type="GeneID" id="76210232"/>
    <dbReference type="OrthoDB" id="9944at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10234">
        <property type="entry name" value="ASKHA_NBD_HSP70_DnaK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000014">
        <property type="entry name" value="Chaperone protein DnaK HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000071">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000003">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00332">
        <property type="entry name" value="DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012725">
        <property type="entry name" value="Chaperone_DnaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02350">
        <property type="entry name" value="prok_dnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="578"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="483"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="590"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH35208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="620" mass="66824" checksum="AF563B46D02AEBF4" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNQ5</accession>
    <name>A0A2S2KNQ5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00740</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875951.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNQ5"/>
    <dbReference type="GeneID" id="76209403"/>
    <dbReference type="OrthoDB" id="1494at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="68" mass="8284" checksum="B1DD60E89E56B1EB" modified="2018-07-18" version="1">MATPTEKNHNKHLDLLQDYKIHLVKYIEELEKLDKQSEFAKKWNEATIKERKYEVQVVDKIIKNLIRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNS1</accession>
    <name>A0A2S2KNS1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNS1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="440" mass="51577" checksum="9B6F85B0815E9F84" modified="2018-07-18" version="1">MLLQQFANTHSMRNEILNLMVEKGIEDDCYVEMLDYTIDLFESQGLGSDYYGYHNIIHELEVTYFALLASIQDKVKITDTDIKYLYVAALFHDFDPQKNVDKPHEESVLKFISMDKKLQQLIDEAKIDLEIIKVLILRTTYPWSGKLKQNAEEQIKQCFEKSDLTRNNLPYQEHIMEMGWYLSVVDRIGGYALGDFSKAMEMAKMNAHALAWRPSLIVRSSVAYFEELLNKETDMAKTILKILPKEMRKNFFGSVLAFMKLRQQEITIQADCSYENVKLIPTIECMSTRNDPKFIKSLYDIFVQLPKPLQFLKDDFEKSVKDPETILNTLRLNDKNGEIIGYSKGGPLERYQLREEIRDENYGLRNTVFLEPLALKMGYWGLKGGSEMRHMFIMQSHSMKYKFLTSFALRDVIRARVDKEQAEFVTLFDPERWDYYRIAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNS8</accession>
    <name>A0A2S2KNS8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PAC2 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875988.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNS8"/>
    <dbReference type="GeneID" id="76209443"/>
    <dbReference type="OrthoDB" id="31247at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610:SF7">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159659">
        <property type="entry name" value="Cgl1923-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="226" mass="25370" checksum="63D09214B42834F5" modified="2018-07-18" version="1">MDFIQNEEPRIEKPIIIAAMQDMGNVGSIVVNFINDSLRTKTFRTAKTTYPTYVIDNGGYIDLPDESWEYRYTKDLIVFGGGKGQPQSSSELNALCQDVIDVAKKYSAKFIYTLGGFHTNVVLDNNPKTFITTTSKELTKQMETLAVNTTPQKSIITGFNGLILGFAKKNQIQGIGMYGEINEPEIPQYRAAISIIKTIEKLTYRKLGDTSQLEAIAQEMDRKFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNU9</accession>
    <name>A0A2S2KNU9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNU9"/>
    <dbReference type="OrthoDB" id="4799at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="191" mass="22466" checksum="F723A9E6D7EFA975" modified="2018-07-18" version="1">MSHNEPNLESYERERIVFQMIRNNPNLHHNALIKLIVPEFMAKTTFEKTRDMLIEKEIITVQTKANMKFYLPSNNYESKSQHLVERNTTNSFHDLKLQIKRLNTDFPHKDIDEKINTVNSLLRNLLETDNGFTILDAAKNPKKTLYRDEHLTIQQLIHSVFEIMRNDRDSEILFPAITSYLGSILPKNSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNX3</accession>
    <name>A0A2S2KNX3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BFN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_00650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNX3"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.690.10">
        <property type="entry name" value="Bifunctional nuclease domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036104">
        <property type="entry name" value="BFN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003729">
        <property type="entry name" value="Bi_nuclease_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02577">
        <property type="entry name" value="BFN_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103256">
        <property type="entry name" value="Hypothetical protein TM0160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51658">
        <property type="entry name" value="BFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BFN" evidence="1">
        <location>
            <begin position="14"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="144" mass="16425" checksum="D6F48AFE4EAEB58D" modified="2018-07-18" version="1">MRNMEIDQAQEPDYESVKIDYVGFVDPYAVEGMVVLKSDTGKEFHMRAFSGEVARHISSFNDPEGESAPSIYKMIEEICEENELILVKVKIYESGEVLRANLYFTGKKDLVLRNYRASDAMALGAFYKIPILVRKNLLKESMEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP05</accession>
    <name>A0A2S2KP05_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PPOX class F420-dependent enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_00420</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP05"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070967">
        <property type="term" value="F:coenzyme F420 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019920">
        <property type="entry name" value="F420-binding_dom_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052019">
        <property type="entry name" value="F420H2_bilvrd_red/Heme_oxyg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03618">
        <property type="entry name" value="Rv1155_F420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35176">
        <property type="entry name" value="HEME OXYGENASE HI_0854-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35176:SF6">
        <property type="entry name" value="HEME OXYGENASE HI_0854-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="PNPOx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyridoxamine 5'-phosphate oxidase N-terminal" evidence="2">
        <location>
            <begin position="3"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="135" mass="15685" checksum="2B44871F12366120" modified="2018-07-18" version="1">MIDAKAVKLFSQKNLVFIATVMKDGSPQVSPVWANYEDGYILVNTAEGRIKHKNVLRDPRVAVSVVSRDNPLDMTTIRGTVEDLIPDYEYKHADKLTQQYMEREHYPFKRDNEKRIILKIKPIKVFVLPELKINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP12</accession>
    <name>A0A2S2KP12_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Roadblock/LC7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866794.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP12"/>
    <dbReference type="GeneID" id="76209437"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046600">
        <property type="entry name" value="DUF6659"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20364">
        <property type="entry name" value="DUF6659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="16999" checksum="A45158D574EC376E" modified="2018-07-18" version="1">MSKGKNSLEKYGNPCITSIVPEKKLDISEYELKCQDILDDDEIRYAALLDESGMILAGGEKPGMTIRLSEEQLQSVCKELASRVSKRKKFDDQLGHVKYSASRREHVVIMSFPIFDKVIMLVAESNVNIDRLAFRVISKLGRQWGEFVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP15</accession>
    <name>A0A2S2KP15_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ADP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
            <ecNumber evidence="6">4.2.1.136</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ADP-dependent NAD(P)HX dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nnrD</name>
        <name evidence="8" type="ORF">NZNM25_01700</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 6 11">
            <text>(6S)-NADHX + ADP = AMP + phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32223"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 6 11">
            <text>(6S)-NADPHX + ADP = AMP + phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32235"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.136" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876038.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP15"/>
    <dbReference type="GeneID" id="76209500"/>
    <dbReference type="OrthoDB" id="15148at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052855">
        <property type="term" value="F:ADP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110051">
        <property type="term" value="P:metabolite repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017953">
        <property type="entry name" value="Carbohydrate_kinase_pred_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592:SF0">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01050">
        <property type="entry name" value="YJEF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 6" id="KW-0520">NAD</keyword>
    <keyword evidence="3 6" id="KW-0521">NADP</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjeF C-terminal" evidence="7">
        <location>
            <begin position="7"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="287" mass="30569" checksum="A4EC4FA70F60F2F1" modified="2018-07-18" version="1">MVRKNLNVTIIKKFIPARNSKSRKGDNGVVLVIGGSYIYHGAPILSSIAALRCGTDLVYTSVPNINVTPTRAISPNLIVIPLVDQKLTRGAVNKLLGSLPRNLDSATIGMGLAIQEKNALLHLVKSLLDRDVRLSLDASALIPDILPLLANKNVVVTPHAGEFKRLFGESPSNSKNERIKLVEKKAKEYGITVLLKGATDVISNGSTTYLYEKKIPAMTVGGTGDVLSGLVAGLLSKNRNSLESAAAATFINGLAGKAVQKKIGLHMTSMDLLDAIPSAMKPFDKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP39</accession>
    <name>A0A2S2KP39_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphoribosylaminoimidazole-succinocarboxamide synthase</fullName>
            <ecNumber evidence="7">6.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">SAICAR synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">purC</name>
        <name evidence="9" type="ORF">NZNM25_00460</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate + ATP = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:22628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SAICAR synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.6" evidence="7"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP39"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00131"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01414">
        <property type="entry name" value="SAICAR_synt_Sc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00137">
        <property type="entry name" value="SAICAR_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001636">
        <property type="entry name" value="SAICAR_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018236">
        <property type="entry name" value="SAICAR_synthetase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00081">
        <property type="entry name" value="purC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43700">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE-SUCCINOCARBOXAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43700:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE-SUCCINOCARBOXAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01057">
        <property type="entry name" value="SAICAR_SYNTHETASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01058">
        <property type="entry name" value="SAICAR_SYNTHETASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAICAR synthetase/ADE2 N-terminal" evidence="8">
        <location>
            <begin position="8"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="278" mass="31963" checksum="994E7F945D3C2AFD" modified="2018-07-18" version="1">MSYCLKFLTRGKVKDLYDVDENTLLFKFSDRVSAFDVKFKQDIPRKGEVLCKFAEFWFNELSVPNHFVRRESDTEIVVKKMKMLPIECVVRGYFYGSLVSRWKQGEVSVPEGTDTTLAAKLPEPIFDPTTKSEHDVPINKSKALDMNLVTEEQYGWLEQASIAIYKKMAAIANNAGFILADLKLEFGILDGKITLGDSIGPDEYRLWPKDSYEVGKIQEAYDKQLLRDWLTANGYQKKFDDERDAGREPIPPEIPDEIIQKMTQRYVLAYEKITGNTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP59</accession>
    <name>A0A2S2KP59_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PUA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_01650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876034.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP59"/>
    <dbReference type="GeneID" id="76209495"/>
    <dbReference type="OrthoDB" id="27972at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21154">
        <property type="entry name" value="PUA_MJ1432-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.10:FF:000017">
        <property type="entry name" value="RNA-binding protein, containing PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.120">
        <property type="entry name" value="Pre-PUA domain, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016437">
        <property type="entry name" value="MCT-1/Tma20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798:SF0">
        <property type="entry name" value="MALIGNANT T-CELL-AMPLIFIED SEQUENCE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798">
        <property type="entry name" value="MCT-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005067">
        <property type="entry name" value="Tma_RNA-bind_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="72"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="158" mass="18029" checksum="38E0204C252172E9" modified="2018-07-18" version="1">MKSNLISKSETSTLLKTVSEKWGIEFPKIKNLKVHQILDDAQIITGEGIKILKINEDYLPFLSETKMLEKFPNVTVDMGAIKFMCKGANVMRPGIKKYTEFEKDQLVCIVEESQHKFLAVGKALVASSELEIMKKGEVIKNIHYISDKFWETGKIIYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP61</accession>
    <name>A0A2S2KP61_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HEPN AbiJ-N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_01480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876017.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP61"/>
    <dbReference type="GeneID" id="76209478"/>
    <dbReference type="OrthoDB" id="382565at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049503">
        <property type="entry name" value="AbiJ_NTD4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18863">
        <property type="entry name" value="AbiJ_NTD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN AbiJ-N-terminal" evidence="1">
        <location>
            <begin position="13"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="289" mass="33437" checksum="A77EB0517021205E" modified="2018-07-18" version="1">MSKKPYFFEKDQKIKFQKNDLDSKTRIGLFNIFVRDVLEKYHFKYKTYGGMWSEKDDGFIAWKNFWIVFLEKSISDISVNHESVLDVLEEIFTNSEHKFLLSIFEYELRKDHLLEDQKDTLSDHVNNFLKKKNVGYRIVGDEISIITREEEIVSLNKSFNTPIDIVNTYMKKANKLLTQDNPDFDGSITNSIHAVESICKKITGESNTTLGQALKIIEKESKIELPTPLKQAFDKIYGWSSSNSGVRHGLSEIPKDKLDFEEAQFMLVACSAFVNYLIDESVKAKISLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP98</accession>
    <name>A0A2S2KP98_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MarR family transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876158.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP98"/>
    <dbReference type="GeneID" id="76209641"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="94" mass="10668" checksum="4C2B650FDA9BD236" modified="2018-07-18" version="1">MQNEQDKQILKVLKDIDSKLSILISLQKTSFTPPKLGAEEKAILKLCNGKNTIKEIMEITSKKKNNVKSTLSHLRKKGIIKSTTMNKKIVYVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPD8</accession>
    <name>A0A2S2KPD8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Inosine-5-monophosphate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_03310</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876180.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPD8"/>
    <dbReference type="GeneID" id="76209669"/>
    <dbReference type="OrthoDB" id="43333at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="18"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="84"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="148"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="240"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="297" mass="33187" checksum="CABC4827C8C05945" modified="2018-07-18" version="1">MKRQPKVLVNIPVHLASIIKQPISISPNTSILDARDIFLRYKIGRLVVELNQKPVGIITEKDLSRSVSVFNQKPVSKILIRDIMTKDLITLQPEASIYDCAKLMQKHGISSIIIKTSKGKLVGVVTKTDLVSTFLIQSTASLPISKIMTKKVITVSPNDSIFEVESVLLNNQIHRVIVTNNKIPVGIITYRDFIPAKTFDLHNEFTDPEERSEIFWNAHLNELNVNKLSYLLTFSAKDIMTKNPFFVSADDVVYTAAIIMIRHGISGLPVIRGKKMVGIITKTDIVNILASKGNPNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPE0</accession>
    <name>A0A2S2KPE0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876157.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPE0"/>
    <dbReference type="GeneID" id="76209640"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="73" mass="8408" checksum="36B993DC8629343D" modified="2018-07-18" version="1">MVKNSEEQMVKKLNEIKDLLMALRDLSMLQASYSDIPTDKISKAAHMQKSRVYEIIPKQKKTSNTKKVNKNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPF6</accession>
    <name>A0A2S2KPF6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01390</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876010.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPF6"/>
    <dbReference type="GeneID" id="76209469"/>
    <dbReference type="OrthoDB" id="3089at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="89" mass="10427" checksum="298E4A7E90E2C9E6" modified="2018-07-18" version="1">MAIFTLPANLEDKIFEIKFGADQTVSKIVSYFPLSESETQKIRSILQNESFDGFHSIFTDKITEDEWNNTKEQIKKKFKDELFDIDKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPG6</accession>
    <name>A0A2S2KPG6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_03230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829025.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPG6"/>
    <dbReference type="GeneID" id="76209661"/>
    <dbReference type="OrthoDB" id="142268at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="283"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="359"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="395"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="422" mass="47262" checksum="37261192F0154BF8" modified="2018-07-18" version="1">MLGGKFSSFSKTAGPGILFACTAIGVSHLVQSTRAGADFGLMMLGFVVLVMLMKYPFFEYGSRYANSTQTSIIDGYKKLGKPALLLYFLLTISSMFFVTGAVGFVTAGFFENLFDVDFIGEWTVVILFAVCVGILAVGKYHVLDSLIKMIVIVLLISTLSAFFFALYHGPTEQIPGFEPKELWDVAGIFFLLALMGWMPTAVDLSSWNSLWTLERMKQTNYKPKLKETLLEFRLAYLITGILAVMFIVLGTFIFYGSGEELPNNNSDFANKVVTLYTETIGDWSYIIIASSAFTVMFGTIIAVFDGYSRSLQRTVELIFTKKENTIRTKFRTFYVLFLVVISVGSLVVIFQFGNNLKELVDFATVLSFVIAPIIAIFNFRLVTGKYLEKEQQPSIFLKILSFTGIIFLSGFAVFFVFMKFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPH3</accession>
    <name>A0A2S2KPH3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carboxylate--amine ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_03330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPH3"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610:SF3">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159659">
        <property type="entry name" value="Cgl1923-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="200"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="240" mass="27376" checksum="FC81E4C71B442DFD" modified="2018-07-18" version="1">MKITKPDTNENTLIVGFPSNGLVGTFSISYLIHYLKMNQIEEIEVPELPSTLFVEGGEILGPIRAYSKKNIFVIISDVPFNQYLAERFALAVHEFCKKYVIKKIIMISGMETINQQNDTSKIYGLASHPSLENILYNNKIMKFLDGSIFGTDAAIISVFRKTKIPVLVLYAECHPFFPDPEASIIAIVTLAKILDIQVDTKDIQSRIDKLRIQHRNLMEQTIRALQEQQEKQTRAPQIYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPH5</accession>
    <name>A0A2S2KPH5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Peptidase M20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_01690</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876037.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPH5"/>
    <dbReference type="GeneID" id="76209499"/>
    <dbReference type="OrthoDB" id="24854at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051458">
        <property type="entry name" value="Cyt/Met_Dipeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005034">
        <property type="entry name" value="PRK06446.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270">
        <property type="entry name" value="BETA-ALA-HIS DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270:SF8">
        <property type="entry name" value="DI- AND TRIPEPTIDASE DUG2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="4">
        <location>
            <begin position="193"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="450" mass="50094" checksum="FA3E56A0B4874C23" modified="2018-07-18" version="1">MKTLQHIDSHMDHLISDLQTLIRQPSVSAKNEGIEECAKLVQRILKKSGISSEILRLKKNVAPIVYGEVKSIQNPKKTLMFYNHYDVQPAEPFDLWDDPPFSGTRKGNKIFGRGATDDKGELITRIKAVEAYLRTTGDVPCNIKFVIEGEEETGSAHIEEYLKKYRKKFSSDGVIWEFGYIDAKNTPIIGLGMKGLLFVELSVSESIRDAHSSLAVLIKNPAWRLIEAVQTLRDSNGNILIKDWYKDVLALSKKDLKIIDDEPFDEDVFKKEFGIRTFLGDKKGLDAKKALVAGATCNIAGFVSGYTGDGAKTVLPGSALVKIDFRLVPKMDPKKQIMRLKNHLKSKGFSDVKIKIFHGEAAARTDSSNPFVSHVKDAADKVFGKSILNVSNAGTGPMHPFVEILKVPCISIGSTYMFSRIHSPNEFARIDLLKKTTKCVCLIMQNFGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPN7</accession>
    <name>A0A2S2KPN7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Chromosome segregation protein SMC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_02360</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPN7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43977">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="4">
        <location>
            <begin position="527"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="820"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="185"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="259"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="399"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="766"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="848"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="834"/>
            <end position="844"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="1176" mass="133634" checksum="E0ACB52B430A9662" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPR3</accession>
    <name>A0A2S2KPR3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphate-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_04200</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the PstS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876298.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPR3"/>
    <dbReference type="GeneID" id="76209768"/>
    <dbReference type="OrthoDB" id="10255at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042301">
        <property type="term" value="F:phosphate ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13565">
        <property type="entry name" value="PBP2_PstS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005673">
        <property type="entry name" value="ABC_phos-bd_PstS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050962">
        <property type="entry name" value="Phosphate-bind_PstS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00975">
        <property type="entry name" value="3a0107s03"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42996">
        <property type="entry name" value="PHOSPHATE-BINDING PROTEIN PSTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42996:SF1">
        <property type="entry name" value="PHOSPHATE-BINDING PROTEIN PSTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12849">
        <property type="entry name" value="PBP_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002756">
        <property type="entry name" value="PstS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PBP" evidence="5">
        <location>
            <begin position="37"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="416" mass="44887" checksum="D05758B5434A0062" modified="2018-07-18" version="1">MRAKITSILLIATLVAILPPLNANASSMPDAPNPNVEFNLTGAGATFPFPLIDLWRVEYKNAYNNVNLNYQSIGSGGGIKQHIEKTVNFAASDAPMKESERELAPGTLHIPESIGGVVVAYNIPEVPNKGLKLTADVVSKIFLGEITKWNDPAITAENPGLNLPAKDIVTAHRSDGSGTTFVFTDYLTTVSPTWDEKIGAGKSVPWPTGLASAGNEGVAGIVKSTEYAIGYIELAYAFQTGMSYASIQNADKTAFIEPTLDSISAASSGVADTLPASEDSWAHVSIVNAPGPDSYPIASFTYLLVYDDLKQVTKNKDQAKAVIHMIHWMITDGQEFSSSLLYVPLADKVVELGKQGLSKVTYDGEILWDYKAASTGSLEIPKWIRDNAKWWSEGLISDQDYINGLQFLIKQGILKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPV8</accession>
    <name>A0A2S2KPV8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nitroreductase family deazaflavin-dependent oxidoreductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876302.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPV8"/>
    <dbReference type="GeneID" id="76209773"/>
    <dbReference type="OrthoDB" id="9717at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004378">
        <property type="entry name" value="F420H2_quin_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04075">
        <property type="entry name" value="F420H2_quin_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="12362" checksum="7F343532005DC690" modified="2018-07-18" version="1">MEIKEELFKPILITKGRNTGNPHSVMLLAVNFNGKIYFSRHRPDGDWFKNAIANPQVKIKYKDRVFSGHAKLITDEEFSKKISELKYPGEKRAKEKRVTIEITLDSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPW7</accession>
    <name>A0A2S2KPW7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Superoxide dismutase</fullName>
            <ecNumber evidence="2 6">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">sodA_2</name>
        <name evidence="9" type="ORF">NZNM25_05210</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the iron/manganese superoxide dismutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="2 6"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876383.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPW7"/>
    <dbReference type="GeneID" id="76209872"/>
    <dbReference type="OrthoDB" id="32917at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.990:FF:000001">
        <property type="entry name" value="Superoxide dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.55.40.20:FF:000001">
        <property type="entry name" value="Superoxide dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.990">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="entry name" value="Iron/manganese superoxide dismutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001189">
        <property type="entry name" value="Mn/Fe_SOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019833">
        <property type="entry name" value="Mn/Fe_SOD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019831">
        <property type="entry name" value="Mn/Fe_SOD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S26, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00081">
        <property type="entry name" value="Sod_Fe_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000349">
        <property type="entry name" value="SODismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01703">
        <property type="entry name" value="MNSODISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00088">
        <property type="entry name" value="SOD_MN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Manganese/iron superoxide dismutase N-terminal" evidence="7">
        <location>
            <begin position="3"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="8">
        <location>
            <begin position="100"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000349-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="206" mass="23171" checksum="BC29F504795A47D3" modified="2018-07-18" version="1">MGKYTLPEMPYAYDALEPHIDARTMEIHHTKHHQAYTDKLNAALEACPADIQSKDIIDILSDINSVPEAQRSAVNFNGGGYDNHRLFWNNMKANGGGEPGGSVADAIKNSFGSFSDFKEKFSSTTAVIQGSGWGWLVYNPSSGKVEYKSMPNQTSPRTEGLVPLLGCDVWEHAYYLNYQNKRPDYIAAWWNVVNWDEVEDRFSKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPY0</accession>
    <name>A0A2S2KPY0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPY0"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="202" mass="24078" checksum="D7F1E039566A1BAD" modified="2018-07-18" version="1">MLITNGFKMKTDMDIILEDSILENASEWIHKTELWRKTKSDKKRCFDTIDYLVKEGFLETKKEQNKHKFRRKNEILTDKEFSESQIRRRGWMVDTVNAINNIEKPLFTYVKSRKQSEPRTKLIKGGLESLDYYMNGSTAHIARLRLAKAYHIINKRTADKRIDVIENTINYIANYFLSTNPDESEQIKEYNQRISRRTTFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPY1</accession>
    <name>A0A2S2KPY1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl18</name>
        <name evidence="7" type="ORF">NZNM25_05170</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">This is one of the proteins that bind and probably mediate the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 50S ribosomal subunit. Contacts the 5S and 23S rRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876379.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPY1"/>
    <dbReference type="GeneID" id="76209868"/>
    <dbReference type="OrthoDB" id="8644at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00432">
        <property type="entry name" value="Ribosomal_L18_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_A">
        <property type="entry name" value="Ribosomal_uL18_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057267">
        <property type="entry name" value="Rbsml_uL18_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005485">
        <property type="entry name" value="Rbsml_uL18_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057268">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006342">
        <property type="entry name" value="PRK08569.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410:SF12">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410">
        <property type="entry name" value="RIBOSOMAL PROTEIN L5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17144">
        <property type="entry name" value="Ribosomal_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="160" mass="17637" checksum="A33679DE1EDACAA4" modified="2018-07-18" version="1">MAYSTILRRLREQKTNYKKRGTMLMGKRDFITVNITNENTQVQIMKPGMTGDKVLASAHSRYLLEKGWKGSRKSVPAAYLTGYIAGKKALSEGAKDAILYTGTRKYTQRMAAALKGVIDAGVAVPADAETFPPDERINGEHLTVKNEVSKIKSAIDSEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPZ7</accession>
    <name>A0A2S2KPZ7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL32</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">50S ribosomal protein L32e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_05240</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPZ7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00513">
        <property type="entry name" value="Ribosomal_L32_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001515">
        <property type="entry name" value="Ribosomal_eL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023654">
        <property type="entry name" value="Ribosomal_eL32_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018263">
        <property type="entry name" value="Ribosomal_eL32_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036351">
        <property type="entry name" value="Ribosomal_eL32_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006332">
        <property type="entry name" value="PRK08562.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L32 AND DNA-DIRECTED RNA POLYMERASE II, SUBUNIT N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01655">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01393">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52042">
        <property type="entry name" value="Ribosomal protein L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00580">
        <property type="entry name" value="RIBOSOMAL_L32E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="53"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="149" mass="17186" checksum="ABCCA62E169A7C5D" modified="2018-07-18" version="1">MYLGTYLFQNSKVKLMPINKDKIAKRQEVKEHNPDFVRPESWRYVRLQTNWRKPKGIDHHQRKQKSRGRPGLVKVGYGGPKEARGLHPSGYTDNLVFNLSDLEKLDPKKDGVRFGHSVGTRKRKEIIVKAIENKFKIFNARVSASGSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ03</accession>
    <name>A0A2S2KQ03_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_04180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ03"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PHOSPHATE-SPECIFIC TRANSPORT SYSTEM ACCESSORY PROTEIN PHOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930:SF2">
        <property type="entry name" value="PHOU DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109755">
        <property type="entry name" value="PhoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="3"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="336" mass="38179" checksum="02419700C30ED289" modified="2018-07-18" version="1">MQLSGGSTYIISLPKNWIEELKIKVGENVTIIKNSNKSLTLIPKEGENQTKKSTAVILSNQKDSGESIKRKIIAAYLSGYKTIKITTKGMRIPAEHSRSIRELVRSTMIGTEIVESSSENIIIQILTRLPELSFETALKRMYLMANNMVLEAIEALEEADTSHADEVANMDDEVDRFGLYMRRNLVLAVGNENFLQDMGLRKPSDCLGHRAIVSRIERIADHASLIAKRTKFIEGKIDSKIFVKIKKLSEKSLEVFEKSIMAVQEHDFEKGEKVAEKVYQVIEEEKQIMSKIKETEKNSTIIRFVLEDLRRIAEYSSDIAEVAIDENIQNIITEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ18</accession>
    <name>A0A2S2KQ18_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_05570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ18"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="122" mass="14011" checksum="B9316DF3F68CE620" modified="2018-07-18" version="1">MQVLRPENRLCQDIIKMDKTIRFVGILCKCGRLKAYDRKKGITPQLTVPETKLVHREALLKAKMNKVFDAKLGKTNWSVESRHNVKWITVYHDKDLILVSTEILSNHDSIVQKIIAMVNAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ50</accession>
    <name>A0A2S2KQ50_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein eL19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl19e</name>
        <name evidence="9" type="ORF">NZNM25_05250</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic ribosomal protein eL19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876386.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ50"/>
    <dbReference type="GeneID" id="76209876"/>
    <dbReference type="OrthoDB" id="11624at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.240:FF:000003">
        <property type="entry name" value="50S ribosomal protein L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01475">
        <property type="entry name" value="Ribosomal_eL19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035970">
        <property type="entry name" value="60S_ribosomal_eL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039547">
        <property type="entry name" value="Ribosomal_eL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000196">
        <property type="entry name" value="Ribosomal_eL19_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015972">
        <property type="entry name" value="Ribosomal_eL19_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057259">
        <property type="entry name" value="Ribosomal_L19e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057260">
        <property type="entry name" value="Ribosomal_L19e_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006343">
        <property type="entry name" value="PRK08570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01280">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25476">
        <property type="entry name" value="Ribosomal_L19e_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01416">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48140">
        <property type="entry name" value="Ribosomal protein L19 (L19e)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL19" evidence="8">
        <location>
            <begin position="4"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="57"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="151" mass="17250" checksum="45FA80AEB48CC1DA" modified="2018-07-18" version="1">MVVNLNAKKRLASRVTGVGIHRIKFDTDHLDDIADAITRENIRSLITANTIKIKPFVGTSRGRAQHKKDQKNKRGITQGSKQGRKGARVGKKEVYVAKVRSLRRLLKIAKDRKDLTNPEFWALYKKVGGNTVRNKAHLRLLMDEIKEKRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ97</accession>
    <name>A0A2S2KQ97_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876297.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ97"/>
    <dbReference type="GeneID" id="76209767"/>
    <dbReference type="OrthoDB" id="1680at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="533" mass="59549" checksum="8CA118577DB1026C" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQC8</accession>
    <name>A0A2S2KQC8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fumarylacetoacetase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_06050</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876455.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQC8"/>
    <dbReference type="GeneID" id="76209958"/>
    <dbReference type="OrthoDB" id="6242at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.850.10:FF:000002">
        <property type="entry name" value="2-hydroxyhepta-2,4-diene-1,7-dioate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051121">
        <property type="entry name" value="FAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42796:SF4">
        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42796">
        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE DOMAIN-CONTAINING PROTEIN 2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="3">
        <location>
            <begin position="79"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="289" mass="32368" checksum="F4685D752EF3BC1A" modified="2018-07-18" version="1">MKIARLSYENNETYGFVNGDNVATKDEITYLTGVPIPQNVKDFLFDGWYDEIKNKIKDLPYAENISKYKLLPPIPNPNKIICLAFNYVDHALEQGLEAPEDPAIVIKPRTTLNSTESDIVCPDFVKQLDYEVELALIIGKNCKNISVKDASSVIFGYMIFNDVSARDIQFKDKQFTRGKSFDSFAPCGPWITTVDEIKNVQNLKLTTKVNGELRQNSSTNNMFIKIPEIISKISHVMTLEKGDIISTGTPAGVMLNKPNAVFLKDGDKVEMEIEGLGILSNTIKFVKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQF8</accession>
    <name>A0A2S2KQF8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Coenzyme F420-0:L-glutamate ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cofE</name>
        <name evidence="2" type="ORF">NZNM25_04790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876348.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQF8"/>
    <dbReference type="GeneID" id="76209831"/>
    <dbReference type="OrthoDB" id="11383at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052618">
        <property type="term" value="F:coenzyme F420-0:L-glutamate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1660.10:FF:000003">
        <property type="entry name" value="Coenzyme F420:L-glutamate ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.100">
        <property type="entry name" value="CofE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1660.10">
        <property type="entry name" value="CofE-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002847">
        <property type="entry name" value="F420-0_gamma-glut_ligase-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47917">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47917:SF2">
        <property type="entry name" value="COENZYME F420:L-GLUTAMATE LIGASE-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01996">
        <property type="entry name" value="F420_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144010">
        <property type="entry name" value="CofE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Coenzyme F420:L-glutamate ligase-like" evidence="1">
        <location>
            <begin position="17"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="256" mass="28041" checksum="7A57A0D2C5EA2C74" modified="2018-07-18" version="1">MQLTVLPLLSERKEERFDVFDALLETLEKNNAELQEGDVLVISTKYISNSQGRIVDLSKIKASQEGLKISKKYQLKPEIAEIIIRESDKIFGGIGGFVITSSDNIMAPNAGIDKSNAKKGNAILYPQNPYLISEQIRRKIFLKMSVHVGIILADSRLMPARIGTSGVAVACAGIEPVLDMRAKKDLDGNPLKVTFQAVVDNIATIANYKMGEGGESKPFAIVRNSEAKLTDRKINPSETAISPDQCVYVRGLSNHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQI2</accession>
    <name>A0A2S2KQI2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_07230</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_264953893.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQI2"/>
    <dbReference type="GeneID" id="76208952"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050736">
        <property type="entry name" value="Sensor_HK_Regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43711:SF1">
        <property type="entry name" value="HISTIDINE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43711">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="9">
        <location>
            <begin position="238"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="204"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH33932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="467" mass="53949" checksum="D2E641604B977A44" modified="2018-07-18" version="1">MIILLLGVVSIYTIKTMDDINQNYNFILEYNQNISNFDDIKIKYERQMTIFESLKSEENLAGVGTFWIYNTEIKNKIYDFNTLIINNPQFVELLEDKKSLELENNIDSYYNLHLNYENSASDALSYFYDGDFGLFLLEYENLKHLQFELFEKLEDIEQTLEIIPVYSKISVEHIIADFQILQWTMIILVGVVTTMLVFFLNQTNSNLKSEIKMQTKNLQKLNEQLKTVDKKREEFISIASHELKGPIQPIFGFVELAKTGIISNQEALEGISNIALNLENIANNVLDLTKIENDELELHLEKNSINELISEVLDSEHFNPDRKVPIITRLDVDVVMNLDRTRIKQVLRNILDNCIKFTETGTITIQTNLLKDKKTLKLFITDSGPEIPHDVLPKIFKKFVTKGNYKVSGFGLGLYISKNIVDAHKGKISAYNKNGHPVFEISLPIISFDLTWKDSESSNLEKNIEYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQL6</accession>
    <name>A0A2S2KQL6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_06120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876462.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQL6"/>
    <dbReference type="GeneID" id="76209966"/>
    <dbReference type="OrthoDB" id="2775at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="90" mass="9805" checksum="DAB204F1E2F5FBBD" modified="2018-07-18" version="1">MGKIRIRTGRGTGTIEVDDDPSKWSGDEYRKIQEARKKAAANRMVHTGRGTGSRKLGDMPDPKARPSTEGMTRVTGRGTSSRKSTNKGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQM1</accession>
    <name>A0A2S2KQM1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">FUR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_05360</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Fur family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876397.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQM1"/>
    <dbReference type="OrthoDB" id="21318at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900376">
        <property type="term" value="P:regulation of secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07153">
        <property type="entry name" value="Fur_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002481">
        <property type="entry name" value="FUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043135">
        <property type="entry name" value="Fur_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33202:SF7">
        <property type="entry name" value="FERRIC UPTAKE REGULATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33202">
        <property type="entry name" value="ZINC UPTAKE REGULATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01475">
        <property type="entry name" value="FUR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="141" mass="16337" checksum="77DD470B993CC2CE" modified="2018-07-18" version="1">MQHLEEIVSSLRDEGFRITPQRIAIVDYLLKTEDHPSAELIHKVVRKRYPMVSLSTVYKTLDLLREKKLVNEIEVEGGARFDAHIDEHINLVCMKCGKIDDVDEDSLKEIQAKAAKKSKYLILKSNFELYGYCHNCKSKMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQQ7</accession>
    <name>A0A2S2KQQ7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TIGR00299 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_05790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876433.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQQ7"/>
    <dbReference type="GeneID" id="76209931"/>
    <dbReference type="OrthoDB" id="10691at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1380">
        <property type="entry name" value="Transcriptional regulatory protein pf0864 domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002822">
        <property type="entry name" value="Ni_insertion"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00299">
        <property type="entry name" value="nickel pincer cofactor biosynthesis protein LarC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36566">
        <property type="entry name" value="NICKEL INSERTION PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36566:SF1">
        <property type="entry name" value="PYRIDINIUM-3,5-BISTHIOCARBOXYLIC ACID MONONUCLEOTIDE NICKEL INSERTION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01969">
        <property type="entry name" value="Ni_insertion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0533">Nickel</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="407" mass="44606" checksum="6BEE98C3C111A74A" modified="2018-07-18" version="1">MVVVIDPQIAGISGDMLLCSLVDLGADKKRVIDGIKKSEKFLSNSSIKKIDFQKIQKHGIESLQLILEIDEETHERKGSEIKKAITDSVKSIGLLEKSQKFAESCIDTLISSESKIHGIPEDSVHFHEASSIDTLIDIVGITIALEDLGLFEEKIVCLPVSVGGGTVTFSHGTMSNPASAILEIFKNSNLIIKGNDSKDELTTPTGACILVNLTNDSVEYYPSMKVDSIGYGAGQKNFETFSNTLKIIRGSQNNFVTDSVKILETNVDDVSGEILGNLIEKIMSKGAKDVSIYHGITKKGRPTNLVSVICDDDKMDDIIDSLVLETGTLGIRISNSNRFIVPRTTHSISLTLNGKSFVVNYKKSTFKGKTDFKIEFDDLKKISNTLGKSIKETESLLRKEIEELEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQR5</accession>
    <name>A0A2S2KQR5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_08030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876657.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQR5"/>
    <dbReference type="GeneID" id="76208872"/>
    <dbReference type="OrthoDB" id="2830at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00156">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050595">
        <property type="entry name" value="Bact_response_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591:SF3">
        <property type="entry name" value="RESPONSE REGULATORY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591">
        <property type="entry name" value="STRESS RESPONSE REGULATOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="5"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="120" mass="13856" checksum="7F776C1A22AB3896" modified="2018-07-18" version="1">MTNTQILLVDDDTQFLEATEYMLKYENYDVVTAKNGEEAIRKYNESHPYIVLMDLKMPIMNGYDAFFKIKKEHPDAKIVFTSAYAINNEEFENAQKSGLFGLLTKPFDLSELKNLISSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQS9</accession>
    <name>A0A2S2KQS9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Inorganic pyrophosphatase</fullName>
            <ecNumber evidence="7">3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pyrophosphate phospho-hydrolase</fullName>
            <shortName evidence="7">PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ppa</name>
        <name evidence="8" type="ORF">NZNM25_08000</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>diphosphate + H2O = 2 phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="7"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876654.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQS9"/>
    <dbReference type="GeneID" id="76208875"/>
    <dbReference type="OrthoDB" id="134160at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00412">
        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.80.10:FF:000003">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00209">
        <property type="entry name" value="Inorganic_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="INORGANIC PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00387">
        <property type="entry name" value="PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="179" mass="20603" checksum="346678643F96E325" modified="2018-07-18" version="1">MSKNFWHDIESGNDIPEIINVIVEIPKGSMNKYEYDKKHNMIKLDRVLFSPFHYPGDYGLIPQSLSDDGDPLDALVLVTNSTYPGILIEARPIGLLQMKDDGKLDDKIICVATNDPRYLHTTDISDIEDHYRSEIAHFFQVYKDLEGKKVEILGWKSAKEAKIVIVESIKRYKDTLKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQV8</accession>
    <name>A0A2S2KQV8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Chemotaxis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_07120</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876578.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQV8"/>
    <dbReference type="GeneID" id="76208963"/>
    <dbReference type="OrthoDB" id="3369at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11386">
        <property type="entry name" value="MCP_signal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.950">
        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089:SF112">
        <property type="entry name" value="LYSOZYME-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089">
        <property type="entry name" value="METHYL-ACCEPTING CHEMOTAXIS PROTEIN MCPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="Methyl-accepting transducer" evidence="4">
        <location>
            <begin position="37"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="5">
        <location>
            <begin position="376"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="50067" checksum="C2EDCD510A40D122" modified="2018-07-18" version="1">MSTQKTKIIDENTILKSESTDNINTNSNSMGTKVLKSFDQAVASSEELSETVTQLESASRSQTSGVEEVSQSLQSIASAIQGVAKNATKAMEMMRQSEEISKTISADAEKGMNKMDSMKSIVSESSNDVKKLAVELSKVDNMTGFITQIAEQTNLLALNAAIEAARAGDVGRGFAVVADEVRRLAENSKKGAEDISELVSSLKNSSDKTTGSIEKGNSIVQDAHEVITNILTSIKNISVSISEVVSQMQEISVATEEVSSGTEEATAASEEVLSVAQTNLESFEDIVKAKDKETKSLKDAYKDARTLSEITNVLDSSTIISTTDVDGISDYVNHFFLDISKFPEEELKGEMHRIFKSDWHDEKLFDLLWKTISSGKIFQTYLRNKTKDGKRFWTKTVVSPTFDENNNVKGYVIIGTPITEIMEMTGMEEACRDIDAGKIVKPQIKEIVEKLKKGNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQW2</accession>
    <name>A0A2S2KQW2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">tRNA pseudouridine synthase D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_08340</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876680.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQW2"/>
    <dbReference type="GeneID" id="76208839"/>
    <dbReference type="OrthoDB" id="1798at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.20:FF:000055">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1510.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.20">
        <property type="entry name" value="TruD, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001656">
        <property type="entry name" value="PsdUridine_synth_TruD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020119">
        <property type="entry name" value="PsdUridine_synth_TruD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011760">
        <property type="entry name" value="PsdUridine_synth_TruD_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042214">
        <property type="entry name" value="TruD_catalytic"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00094">
        <property type="entry name" value="tRNA_TruD_broad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326:SF21">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE PUS7L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01142">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037016">
        <property type="entry name" value="Pseudouridin_synth_euk_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50984">
        <property type="entry name" value="TRUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01268">
        <property type="entry name" value="UPF0024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TRUD" evidence="4">
        <location>
            <begin position="153"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="398" mass="45413" checksum="A3A8B35291736865" modified="2018-07-18" version="1">MIPNLDSQIGITVYSTNFSGIGGKIRAKPEDFHVSEIISDKVNKSITDQEGYAVYKLKKKKIDTNHALSGIFRKKGIRLKSLGLKDASAITEQFVCSGNKGKAIEDFSTDKYSLEKIGYVKKPLSKKDMIANHFKIKISDCSNDLSSFTEYDKVLNFYGYQRFGSKRPVTHLIGKAILQRDFKKAVDLILSFTSSYDSKENTEIREKLSDKANFEKYFDQVPSQMDVEKIVLKEMIEHDDYFAAIRAIPLSLRRFYIQAYQSFIFNQSLSMAFLDGENLFEAQPGDVCFDFNGIIGKYVKGLDQRLALPFVGYSYYKKTRFDHQISKILSNEEITPKDFFLKEMQEVSNEGGFRQAALHCFDYSSQGNTVEFSLSRGMFATILLREIMKPEDPMTAGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQX7</accession>
    <name>A0A2S2KQX7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_08630</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQX7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="11"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="79" mass="8515" checksum="F7B96CD0A4ED5229" modified="2018-07-18" version="1">MRQHTHKLETVAKKSNVPISVKIKQGTSIVTEIINIAKSSRTDLVVLGSHGRTGVKRMILGSVANGVVQKSKCPVLIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR03</accession>
    <name>A0A2S2KR03_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Phosphoribosylamine--glycine ligase</fullName>
            <ecNumber evidence="2 10">6.3.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">GARS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8 10">Glycinamide ribonucleotide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 10">Phosphoribosylglycinamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 13" type="primary">purD</name>
        <name evidence="13" type="ORF">NZNM25_07780</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-phospho-beta-D-ribosylamine + glycine + ATP = N(1)-(5-phospho-beta-D-ribosyl)glycinamide + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17453"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 10">Belongs to the GARS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.13" evidence="2 10"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR03"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00125"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000006">
        <property type="entry name" value="phosphoribosylamine--glycine ligase, chloroplastic-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.600.10:FF:000001">
        <property type="entry name" value="Trifunctional purine biosynthetic protein adenosine-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.10">
        <property type="entry name" value="Phosphoribosylglycinamide synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00138">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020561">
        <property type="entry name" value="PRibGlycinamid_synth_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000115">
        <property type="entry name" value="PRibGlycinamide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020560">
        <property type="entry name" value="PRibGlycinamide_synth_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037123">
        <property type="entry name" value="PRibGlycinamide_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020562">
        <property type="entry name" value="PRibGlycinamide_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00877">
        <property type="entry name" value="purD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472">
        <property type="entry name" value="PHOSPHORIBOSYLAMINE--GLYCINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLAMINE--GLYCINE LIGASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01071">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02843">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02844">
        <property type="entry name" value="GARS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01209">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01210">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 10" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="12">
        <location>
            <begin position="105"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH33987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="421" mass="46054" checksum="6DD1892EEA23F94F" modified="2018-07-18" version="1">MLANILVVGSGGREHALSWKLSQSSKVETVFTSPGNGGTENNVPIDVTNLDELADFAQKNNCFTVVGPEVPLAAGIVDKFNKLNLKIFGPSQMAAQLESSKIWAKNFMKRNDIPTARFEIFDDAQKAEDYVNSLEYNVVVKADGLAAGKGVIVCNSNNEAISAIQTILVKKTFGDAGNRIIVEERLDGIEASYIALSDGEIAIPMASSQDHKRIFDNDDGPNTGGMGAYSPTPVIDDVLAEKIQKKIIDKTIRSMKNEGIVFKGFLYAGIMIRDNEPYVLEYNVRMGDPECQPIVMRMDFDLYDYFVASVEGKLSSMPLPSWKNQYAVCVVLASDGYPESYVKGEEISGFDSISNQAYVFHAGTKKIDGKIVSDGGRVLGVTALGESLEIAINNVYDATEKITWAHKYCRKDIGKKGLAYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR05</accession>
    <name>A0A2S2KR05_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">L-aspartate dehydrogenase</fullName>
            <ecNumber evidence="6">1.4.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">nadX</name>
        <name evidence="9" type="ORF">NZNM25_08800</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-aspartate + NAD(+) + H2O = oxaloacetate + NH4(+) + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11788"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.4.1.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-aspartate + NADP(+) + H2O = oxaloacetate + NH4(+) + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11784"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.4.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="6">The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the L-aspartate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.21" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876718.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR05"/>
    <dbReference type="GeneID" id="76208790"/>
    <dbReference type="OrthoDB" id="15415at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00456"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033735">
        <property type="term" value="F:aspartate dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016639">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01265">
        <property type="entry name" value="NadX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002811">
        <property type="entry name" value="Asp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022487">
        <property type="entry name" value="Asp_DH_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020626">
        <property type="entry name" value="Asp_DH_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011182">
        <property type="entry name" value="L-Asp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03855">
        <property type="entry name" value="NAD_NadX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009828">
        <property type="entry name" value="PRK13303.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009829">
        <property type="entry name" value="PRK13303.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009830">
        <property type="entry name" value="PRK13304.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31873:SF6">
        <property type="entry name" value="ASPARTATE DEHYDROGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31873">
        <property type="entry name" value="L-ASPARTATE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01958">
        <property type="entry name" value="Asp_DH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005227">
        <property type="entry name" value="Asp_dh_NAD_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="3 6" id="KW-0521">NADP</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aspartate/homoserine dehydrogenase NAD-binding" evidence="8">
        <location>
            <begin position="8"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartate dehydrogenase" evidence="7">
        <location>
            <begin position="170"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="272" mass="29189" checksum="DD4CE5DC87686E7B" modified="2018-07-18" version="1">MKRIGLLGCGAIGTQIALAIDTGKIPGVLTHIYDNSKYAATELASKLNNKPLIVENSHLLSSNPIDIIVEAASQDAVKDVALSVLQNKRDLMIMSVGALLDESIYDVLSDACNHFKKTIYLPSGAIAGLDGLKSVKDELESVSITTTKHPRSLKGAKFFETSDINLDAIDSSTIIFEGTAKEAVSMFPANINVAALLSLCGIGSEKTIVKIIADPNTDKNTHHIEAKGKFGKMTFTIENFPDPNNPKTSRLAILSAIETLRKYCSDDIQIGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR11</accession>
    <name>A0A2S2KR11_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative ATP-dependent helicase YqhH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">yqhH</name>
        <name evidence="4" type="ORF">NZNM25_07620</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_160049202.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR11"/>
    <dbReference type="GeneID" id="76208914"/>
    <dbReference type="OrthoDB" id="6396at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="2">
        <location>
            <begin position="2"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="3">
        <location>
            <begin position="275"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="519" mass="60135" checksum="79D311239B839210" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR19</accession>
    <name>A0A2S2KR19_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876728.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR19"/>
    <dbReference type="GeneID" id="76208780"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="73" mass="8135" checksum="86E6FC770B574547" modified="2018-07-18" version="1">MSLRNHGFRAVLKGINKHGDHSRATKKKSKNVGEQPRTEDLMGKMLSEMSSKIDAVETSLTEVQKKKVKLLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR53</accession>
    <name>A0A2S2KR53_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_09240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876759.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR53"/>
    <dbReference type="GeneID" id="76208746"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="201" mass="23818" checksum="35A361D17E406A90" modified="2018-07-18" version="1">MQDFEVEFKVLGEFPKYLKPQVTDKIKIVKEKLNADDDSLSETILSVSISSESELTAEYYARKTLSRFLSFYSVTTLNHIRILDDYNEPITIRSIKDGKKELHYPIAYSLEEYEQQFFLDHVLPYYDIILKKGNEYLKIASEYLWRVRFEESVEVCMINCFIALEQDHHWTAEGLDGIFQQINRMKMYLDKDLPRILKESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR78</accession>
    <name>A0A2S2KR78_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876678.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR78"/>
    <dbReference type="GeneID" id="76208841"/>
    <dbReference type="OrthoDB" id="3291at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="69" mass="7437" checksum="16E41C55E10651EC" modified="2018-07-18" version="1">MRKGIFIVIIGGIISVFGIIFHLQGQSVVGPESSFMYSNPDWITYGIQIVITGLIMIGIGIAITVLKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRJ5</accession>
    <name>A0A2S2KRJ5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_09520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876904.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRJ5"/>
    <dbReference type="GeneID" id="76208718"/>
    <dbReference type="OrthoDB" id="8756at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="199" mass="23420" checksum="665C8883A481C4C2" modified="2018-07-18" version="1">MLEENLSKRQHMMQKEPTRSITYRLPEKLVLELENESTQKGISQNVLVRQIFEKYVQWDRFSGKIGMIPVPKGILESLGVELDEKDIDEIITLMFPVIKDTVLFIKGDYDLKRCIETLEDYMRSSGMNSDHRIEGDMHIFLIQHELGIKWSMFTEKLLTHVFRNFLPDKELKFQTTDTTVILSVSLGSDFDEHDYHTKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRK3</accession>
    <name>A0A2S2KRK3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_10770</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876932.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRK3"/>
    <dbReference type="GeneID" id="76208582"/>
    <dbReference type="OrthoDB" id="11880at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="72" mass="7903" checksum="F6B8489246100DBB" modified="2018-07-18" version="1">MTGDESSKISIREIVLKGTIIALIVTIPSLSTFVLIWVLLDDLFLGVILGAFVHFIAMGFSLKISKKILVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRN8</accession>
    <name>A0A2S2KRN8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_11040</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876953.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRN8"/>
    <dbReference type="GeneID" id="76208554"/>
    <dbReference type="OrthoDB" id="26307at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11717">
        <property type="entry name" value="THUMP_THUMPD1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2300.10">
        <property type="entry name" value="THUMP superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040183">
        <property type="entry name" value="THUMPD1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13452">
        <property type="entry name" value="THUMP DOMAIN CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13452:SF10">
        <property type="entry name" value="THUMP DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="2">
        <location>
            <begin position="59"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="173" mass="19719" checksum="D56030096510C9E5" modified="2018-07-18" version="1">MNLIITCARHLETETEDELRDILEEYGDSDAKISITNMSGILTAETNLNPVEVVGKMKEMLLDEPWSIRYCLRVIPIQRIVETKIEEIEKIISEDSKQILDGETYRISIEKRNSDISSQEIISKIANKIKNKVSLEFPDKIILIEILGNKTGISILKKSDILSVEKTKRSISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRN9</accession>
    <name>A0A2S2KRN9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_10750</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876930.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRN9"/>
    <dbReference type="GeneID" id="76208584"/>
    <dbReference type="OrthoDB" id="6817at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="132" mass="15305" checksum="0B32CC75CC4FAC54" modified="2018-07-18" version="1">MSVDTETCKHQPVYFGVVNINIDERTIGSVDVWRCGVCKKRFCEEKQLGIEEIADLVGMPKIDPDAKWAVTICKLQQGKYKWKLVRLTENGEIKHECLDEQIIPLKINNFKIEDDKHWSFLIDDNINKAVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRS6</accession>
    <name>A0A2S2KRS6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9 10">DNA repair and recombination protein RadA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">radA</name>
        <name evidence="15" type="ORF">NZNM25_10580</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9 11">Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9 11">Belongs to the eukaryotic RecA-like protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876870.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRS6"/>
    <dbReference type="GeneID" id="76208604"/>
    <dbReference type="OrthoDB" id="31129at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002052">
        <property type="entry name" value="DNA repair protein RAD51 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00348">
        <property type="entry name" value="RadA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011938">
        <property type="entry name" value="DNA_recomb/repair_RadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016467">
        <property type="entry name" value="DNA_recomb/repair_RecA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010995">
        <property type="entry name" value="DNA_repair_Rad51/TF_NusA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020587">
        <property type="entry name" value="RecA_monomer-monomer_interface"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003301">
        <property type="entry name" value="PRK04301.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02236">
        <property type="entry name" value="recomb_radA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942:SF30">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN DMC1_LIM15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942">
        <property type="entry name" value="RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005856">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47794">
        <property type="entry name" value="Rad51 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50163">
        <property type="entry name" value="RECA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9 11" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6 9 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="13">
        <location>
            <begin position="84"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="RecA family profile 2" evidence="14">
        <location>
            <begin position="260"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="320"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="325"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="342"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="369"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="NCBIfam" id="TIGR02236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR005856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GBH34267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="379" mass="41478" checksum="EE186351945C50D6" modified="2018-07-18" version="1">MVEDLRLDSLEGVGPVTTRKLSDAGVHNVMDLIVRGPVEIAEITGMEKDTAEKIVNKARQHLVDGGLIAKHFVSATEIYKHRQSIGKITTGTNCLDTLFDGGIETQALTEVYGEFGCGKTQFAHTMSVMVQKTKEEGGLEGSVLYIDTENTFRPERIVSIAKAHDMDPEKVLDNIIVARAYNSAHQVLILEEAGPIIEENNVKLIVADSAVGLFRAEYLGRGTLSVRQQKLNHFVHLLSRIAETYNCAAIATNQVMASPDVFFGDPTRPIGGNVVAHTSTYRIYFKKSGKKRIARMVDSPHHPEEEVIFALGEAGVIDPEDADKKTKKTTKKTTKPAKETAEVDATETAEVDATETAEVDNIVKSTADNESEDSEPIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRV2</accession>
    <name>A0A2S2KRV2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11770</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877010.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRV2"/>
    <dbReference type="GeneID" id="76208475"/>
    <dbReference type="OrthoDB" id="4293at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="194" mass="22662" checksum="567006D3B4BC200C" modified="2018-07-18" version="1">MANDTSDLIDTLYKKDNSIQSEHISQQIIRKSPKAKYQIFDESEFTHGRKVLGNLIVHGMIASGGTDIDWLIEHKGGFVILEFKGFHNDKINIAKGQMIAYEKLHEKLNQTTKCYLYVVGCDDIDFSNPDSTVWVFEMSQWRDGIIPKNTIDIYSEENKQNKYIVYREYMEEISVEKLRDLVDSHWNEFEKITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRX8</accession>
    <name>A0A2S2KRX8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">site-specific DNA-methyltransferase (adenine-specific)</fullName>
            <ecNumber evidence="2">2.1.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_11550</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15197"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="2.1.1.72"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the N(4)/N(6)-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.72" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876995.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRX8"/>
    <dbReference type="GeneID" id="76208497"/>
    <dbReference type="OrthoDB" id="372040at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1020.10:FF:000003">
        <property type="entry name" value="Site-specific DNA-methyltransferase (adenine-specific)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1020.10">
        <property type="entry name" value="Adenine-specific Methyltransferase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023095">
        <property type="entry name" value="Ade_MeTrfase_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012263">
        <property type="entry name" value="M_m6A_EcoRV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012327">
        <property type="entry name" value="MeTrfase_D12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00571">
        <property type="entry name" value="dam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30481">
        <property type="entry name" value="DNA ADENINE METHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30481:SF3">
        <property type="entry name" value="DNA ADENINE METHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02086">
        <property type="entry name" value="MethyltransfD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000398">
        <property type="entry name" value="M_m6A_EcoRV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00505">
        <property type="entry name" value="D12N6MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="286" mass="32819" checksum="0B528D65E0868FE3" modified="2018-07-18" version="1">MKQQYGQILVTPKPFVKWAGGKRQLIPILNENLPKTFGTYFEPFLGGGALLFHMLTERNGQKCSISDLNSDLVLSYITIRDRIDDLISSLKSHEKNYQKDSKSYYYSVRESNPRNEIEKTSRLIFLNRTCFNGLYRVNSKGKFNVPIGKYTNPNIVNEDNLRSVSSILQSSKASIKCRDFEAVLRDAKKDDLVYFDPPYQPVSNTANFTSYTNKDFTFDDLNRLAELCTKLDSKGCRVLLSNSNSKEVAEIFSNEPWKISKIQANRSINSNSKKRTGHFELLIKNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRY4</accession>
    <name>A0A2S2KRY4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_12040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877053.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRY4"/>
    <dbReference type="GeneID" id="76209302"/>
    <dbReference type="OrthoDB" id="350295at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050697">
        <property type="entry name" value="Adenylyl/Guanylyl_Cyclase_3/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43081:SF1">
        <property type="entry name" value="ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43081">
        <property type="entry name" value="ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="1">
        <location>
            <begin position="81"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="269" mass="30485" checksum="AFF6AA69D8B17A43" modified="2018-07-18" version="1">MTENIDKNKDEKLSKTSMDNTDVVDMLLSKNKEQTVDFETMILETQKRVWGSLKKGYEYSGMADESEKFLRKNVFLKLDMVVLYVDLVGSTTMTLEMPAEKIAIIVSSFSQEMAAVIRQHHGYVLKFVGDAVIGYFVAEGNSLLAADNAVNCAKSMITVIQKGINPILNQYDYPDLMVKIGIDFGQNIVVRYGSDAEQSHVDLMGPAMNIAAKIQNIAKPNQILIGNDVYQKLHPTSQKNFLQIVWKNNEWKYRSRLTGEIYKIYEFKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRZ6</accession>
    <name>A0A2S2KRZ6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Adenylosuccinate lyase</fullName>
            <ecNumber evidence="8">4.3.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_11280</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes two reactions in de novo purine nucleotide biosynthesis. Catalyzes the breakdown of 5-aminoimidazole- (N-succinylocarboxamide) ribotide (SAICAR or 2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate) to 5-aminoimidazole-4-carboxamide ribotide (AICAR or 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) and fumarate, and of adenylosuccinate (ADS or N(6)-(1,2-dicarboxyethyl)-AMP) to adenosine monophosphate (AMP) and fumarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + fumarate</text>
            <dbReference type="Rhea" id="RHEA:23920"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:23921"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N(6)-(1,2-dicarboxyethyl)-AMP = fumarate + AMP</text>
            <dbReference type="Rhea" id="RHEA:16853"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:16854"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer. Residues from neighboring subunits contribute catalytic and substrate-binding residues to each active site.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.2" evidence="8"/>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876971.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRZ6"/>
    <dbReference type="GeneID" id="76208529"/>
    <dbReference type="OrthoDB" id="7033at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00132"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00336"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070626">
        <property type="term" value="F:(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004018">
        <property type="term" value="F:N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01360">
        <property type="entry name" value="Adenylsuccinate_lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000014">
        <property type="entry name" value="3-carboxy-cis,cis-muconate cycloisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019468">
        <property type="entry name" value="AdenyloSucc_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004769">
        <property type="entry name" value="Pur_lyase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00928">
        <property type="entry name" value="purB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43172">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43172:SF1">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10397">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00145">
        <property type="entry name" value="ARGSUCLYASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00998">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Adenylosuccinate lyase C-terminal" evidence="9">
        <location>
            <begin position="362"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="NCBIfam" id="TIGR00928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="453" mass="50682" checksum="22F1FDB61696CE53" modified="2018-07-18" version="1">MAILPIDSGRYGTKEMLIIFSEQKKVDYQLEIEGAAAISQSEIGMISKSVGKEIFKIAVSGKISAKRIKQLEVKSDHDTAALVESLSEKCSKNARPWIHYGLTSNDLVDTSNSMQMRDALQIIEPKVAKMASILAKKAIKYEKIPAVGRTHGQHASIISFGLKFANWAAEMAKHVERIEEIKKRILICKTLGVVGTGSLMGAKSLEVQKRVAKRLKLFPAEVTTQVIPRERYAEYVFELALIGATLEKIAIEIRNLQRTEIAEVAEQFKKGQMGSSAVPVKRNPIKSERVSSLSKLVRSQVAVSFENIPLWHERDLSNSANERFVIPTVSILVDEMLETMTRIVSNLMVNEKRIVDNLYITKGQIFAEFVLEALIKKGIPRFVAYKDVQRVAFEANDKGMQYIDAIKKDKAFSSKLTDKEIDSIFSPEKHLGASSAIIKNVEKSVQNTIRKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS01</accession>
    <name>A0A2S2KS01_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12510</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_160049217.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS01"/>
    <dbReference type="GeneID" id="76209253"/>
    <dbReference type="OrthoDB" id="354at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="52" mass="5956" checksum="E15AEF05520165E3" modified="2018-07-18" version="1">MAFKKGIHKCVECEKKQKEDPQDMGLCSKCSATETRLEKTIYKEMINDGSFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS04</accession>
    <name>A0A2S2KS04_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Peptidase A24</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_12240</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877071.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS04"/>
    <dbReference type="GeneID" id="76209280"/>
    <dbReference type="OrthoDB" id="19094at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052218">
        <property type="entry name" value="Preflagellin_Peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009655">
        <property type="entry name" value="Preflagellin_peptidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000045">
        <property type="entry name" value="Prepilin_IV_endopep_pep"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36506">
        <property type="entry name" value="PREFLAGELLIN PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36506:SF1">
        <property type="entry name" value="PREFLAGELLIN PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06847">
        <property type="entry name" value="Arc_PepC_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01478">
        <property type="entry name" value="Peptidase_A24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Prepilin type IV endopeptidase peptidase" evidence="7">
        <location>
            <begin position="15"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Preflagellin peptidase C-terminal" evidence="8">
        <location>
            <begin position="172"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="230" mass="25565" checksum="DB47AFB22FA06A76" modified="2018-07-18" version="1">MDLLPDYHIVGMGLALIMLVVASIIDVLKREIHDYYWIVFGIAGFLLIFFSPDLMSDLFTLGFALIIAPIVILMWRIGLFGGADAFALITLAIIAPMATLSENPITPFTTLSNAVLLFVIPFIVNVLRNTIAQLKGENIFEGFDETTGKKIVAILIGYRAKNPKFGFSIEKTKKGRKRLDLKLHHAENHEFCTKPDTWVTPGVPYLPLITGGFLIQLFFGDMLLGTFLGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS39</accession>
    <name>A0A2S2KS39_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_10590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876871.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS39"/>
    <dbReference type="GeneID" id="76208603"/>
    <dbReference type="OrthoDB" id="379419at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="82" mass="9596" checksum="89183FDAFFFE7C4B" modified="2018-07-18" version="1">MVQKEFGCDNNGCNHTIVVTSPDDVYTRLLRDVCCDESMEKKYECDDCHFMNTRYWCVKHPSHVIGFGAKDPYDETRFSTYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS50</accession>
    <name>A0A2S2KS50_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator TrmB N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_13010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS50"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051797">
        <property type="entry name" value="TrmB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="1">
        <location>
            <begin position="45"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="297" mass="33400" checksum="1C6D8355DD332EE4" modified="2018-07-18" version="1">MIGINTKIILESMQQQDLDPKNFRKSELGIIEHVNDVSLEFSSMLGLEFQAGQIYMTLLRVGPTTTGALAKEMKIDRNKIYRVIEDLVSAGFVSMTISSPKLCIPTDPENAIELVLNKKKKEIDKINKFKKEMIQKIDGVITAPFGTSIPTFRVIQGLNNVYSSIGISIENSSDVVYIVTSIKDIIRMYHSDIPEKIKICTKNGGKVFVITNSDEKKMLPYIKRLNATEVKIGKLPSNGRILAFENKQMFMSDSTNVNDFMDESSDISLCTDATEMTHNITKLCQFLWEVSTPINLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS52</accession>
    <name>A0A2S2KS52_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829080.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS52"/>
    <dbReference type="GeneID" id="76209238"/>
    <dbReference type="OrthoDB" id="5004at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="51" mass="6355" checksum="8D2712B2B30423F3" modified="2018-07-18" version="1">MHCDDKRTLYVLKEEIERKWNELRDTGFKDKVLLKNLNDAFLDYFEYKNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS62</accession>
    <name>A0A2S2KS62_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Universal stress protein UspA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_12740</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_160049220.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS62"/>
    <dbReference type="GeneID" id="76209227"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006276">
        <property type="entry name" value="UspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="6"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="148" mass="16709" checksum="F50984208D6261DB" modified="2018-07-18" version="1">MIQNRIKKIVIPFDGSIHSKHAFNMALNLAQKYNSKLILVTCVEKINGSWYGKEFAPSYNKDVKRYKEKILRETSKLEEISKKKKIKTMTKIFVTDSIVEQILSFSKSNNADVIVMGSHGRSGIDKLILGSVANGVVQRSKIPVLVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS65</accession>
    <name>A0A2S2KS65_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Roadblock/LC7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13210</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877146.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS65"/>
    <dbReference type="GeneID" id="76209177"/>
    <dbReference type="OrthoDB" id="1734at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046600">
        <property type="entry name" value="DUF6659"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20364">
        <property type="entry name" value="DUF6659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="13969" checksum="99FDEDCEA0086CD0" modified="2018-07-18" version="1">MSAKIYDYGKICKSVLSIDPKIRFAGVINERGRLVAGGMRENVEPLESEKDDEMIFMELALRVKMRKEFDKQLGPVNFAMASRERALAISVLINEDILYVVSEPDSDYGVLPKKIIEIIHSQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS85</accession>
    <name>A0A2S2KS85_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877065.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS85"/>
    <dbReference type="GeneID" id="76209286"/>
    <dbReference type="OrthoDB" id="11458at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="74" mass="8688" checksum="E1011D4C1111F0D6" modified="2018-07-18" version="1">MSEKNKDELIDAQKQVIGILFEVIKRLQANNDLDDEYFKIISEEIKDETRLQQILNERSENAKIAGRLLEQLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS99</accession>
    <name>A0A2S2KS99_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA/RNA-binding protein Alba-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_13170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877141.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS99"/>
    <dbReference type="GeneID" id="76209182"/>
    <dbReference type="OrthoDB" id="10360at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Alba-like" evidence="1">
        <location>
            <begin position="22"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="104" mass="11283" checksum="13397D7FB5AE6F75" modified="2018-07-18" version="1">MLMEETTEQYGHEQTEKSEYTIINIGNDPVMQSAIDVLSTLGSKHKVILKSRGNSIPNAVAVANIITEKMLKGNSKIQKINLDTVAAPGIGSMTSTIEIIINKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSB0</accession>
    <name>A0A2S2KSB0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Asparagine synthetase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_11290</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSB0"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050795">
        <property type="entry name" value="Asn_Synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772">
        <property type="entry name" value="ASPARAGINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772:SF2">
        <property type="entry name" value="ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Asparagine synthetase" evidence="3">
        <location>
            <begin position="11"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="253" mass="28958" checksum="47CC2EB68432894A" modified="2018-07-18" version="1">MAMNELNKKLLENIKDSISETVQKKKIGIAFSGGVDSTLISKICYNMNYDVVLLTIGFSESHDILFAKQVNEYLKYPHHILEIDSKTFPDVSLKIKQKIQTENLSWNENCIAFYYVSKLANRLELDTVITANGIDELFCGYNAYREAFSEGESKIHEVMITKLNNELKMMKAVNLIASEFGVKILQPLLSSKFIEYAKTIPISEKIHDSEDLYRKHIIRKLASDIKVPELSCTKRKKALQYGSKIHKALLKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSE5</accession>
    <name>A0A2S2KSE5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nicotinamide-nucleotide adenylyltransferase</fullName>
            <ecNumber evidence="4">2.7.7.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">NAD(+) diphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">NAD(+) pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">NMN adenylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_13730</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>beta-nicotinamide D-ribonucleotide + ATP + H(+) = diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21360"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="EC" id="2.7.7.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the archaeal NMN adenylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.1" evidence="4"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSE5"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00600"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00243">
        <property type="entry name" value="NMN_adenylyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006418">
        <property type="entry name" value="NMN_Atrans_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002243">
        <property type="entry name" value="PRK01153.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342:SF0">
        <property type="entry name" value="BIFUNCTIONAL NMN ADENYLYLTRANSFERASE_NUDIX HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342">
        <property type="entry name" value="PHOSPHOPANTETHEINE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 4 6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="5">
        <location>
            <begin position="2"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="171" mass="19393" checksum="E88C23C0EFE4B5E0" modified="2018-07-18" version="1">MGRFQPFHLGHLDLATRILDECDEVIIAITSSQFNYLEKDPFTAGERIEMIHNSLKEASLDLSRCFVVSIENQFNVATWASYLKSALPHFDKVFSGNNYVSMILADSGIEVMEPMFLDRTLYNATKIRSMIVSGENWKDSVPNAVYELLTKINAKNRLTVISKSDTNPTKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSF3</accession>
    <name>A0A2S2KSF3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Methylmalonyl-CoA mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_13600</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877174.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSF3"/>
    <dbReference type="GeneID" id="76209140"/>
    <dbReference type="OrthoDB" id="38408at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004494">
        <property type="term" value="F:methylmalonyl-CoA mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.240">
        <property type="entry name" value="Methylmalonyl-CoA mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016176">
        <property type="entry name" value="Cbl-dep_enz_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006099">
        <property type="entry name" value="MeMalonylCoA_mutase_a/b_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006098">
        <property type="entry name" value="MMCoA_mutase_a_cat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00641">
        <property type="entry name" value="acid_CoA_mut_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101:SF1">
        <property type="entry name" value="METHYLMALONYL-COA MUTASE, LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101">
        <property type="entry name" value="METHYLMALONYL-COA MUTASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01642">
        <property type="entry name" value="MM_CoA_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51703">
        <property type="entry name" value="Cobalamin (vitamin B12)-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methylmalonyl-CoA mutase alpha/beta chain catalytic" evidence="2">
        <location>
            <begin position="18"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="533" mass="59970" checksum="ACAB7B08DB666E92" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSM9</accession>
    <name>A0A2S2KSM9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_14570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877259.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSM9"/>
    <dbReference type="GeneID" id="76209038"/>
    <dbReference type="OrthoDB" id="11575at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="116" mass="12668" checksum="FA9458532B47D298" modified="2018-07-18" version="1">MVDPLKERLANLKKISDSAKRRANLYTDIAKIDSKHTNSSLGALQKAPAPGKKLQKIGFLMLWIPEPTGVTCAVGGPMIVAGRYLDKKYNGSTIHDIGHETKNTLSSISDFKNSIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSQ0</accession>
    <name>A0A2S2KSQ0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_14450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSQ0"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="108" mass="12421" checksum="A565CB26586D6B7C" modified="2018-07-18" version="1">MLFGQTIPGVVLAFFKVAGEYVILGAVFIFALAWLLKAKPHNRPKQYSVVVFDVFGKISHIDGIRTEFKTHDVAWSFMKQYKEAYPLYNFALVSELPKSDKPTIFRYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSV0</accession>
    <name>A0A2S2KSV0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_15140</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877308.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSV0"/>
    <dbReference type="GeneID" id="76208982"/>
    <dbReference type="OrthoDB" id="9652at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032993">
        <property type="term" value="C:protein-DNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00156">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="REGULATOR OF RPOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111:SF1">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ORR33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="4"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="156" mass="18276" checksum="E70AEAD5C702D75B" modified="2018-07-18" version="1">MVKKLLIAEDNKFTAMQYKKFFEANGYDVDIFNNGAICLQKYENELRYRKIVLKDKTPPYDYVLLDQDMPKMTGTVVSEKIHKQCPRQRIIFLSAYGQNIMQSNESTKDSYLQIMQKPFSLEFLLKKITPRSFSSIKRSNQENNLLTATQSPETIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSX3</accession>
    <name>A0A2S2KSX3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_15440</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877417.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSX3"/>
    <dbReference type="GeneID" id="76208444"/>
    <dbReference type="OrthoDB" id="2835at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="177" mass="20652" checksum="943B7EB03355628D" modified="2018-07-18" version="1">MIITMMVIMNQSEETGSMEVEDAMDKELVPVEENAEVQEKLDEIEKINLENEYSPKEREWLTSGPFQIDRSEYVLGEKIFLRINGISYDEKGQIVFLRPLNSSHYSVYWTIPFDGAERPAFNYYLEPQLSKIKGYCSVEDFIGDWRVVFRGTDYPNLEFKITEDILPGEEDSYESVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSY7</accession>
    <name>A0A2S2KSY7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">aspartate-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="4">1.2.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_15810</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the aspartate-semialdehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.11" evidence="4"/>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877409.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSY7"/>
    <dbReference type="GeneID" id="76208406"/>
    <dbReference type="OrthoDB" id="38238at2157"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00016"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00463"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00464"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004073">
        <property type="term" value="F:aspartate-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18130">
        <property type="entry name" value="ASADH_C_arch_fung_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02315">
        <property type="entry name" value="ScASADH_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051823">
        <property type="entry name" value="ASADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000319">
        <property type="entry name" value="Asp-semialdehyde_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005676">
        <property type="entry name" value="Asp_semi-ald_DH_pep-lack"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00978">
        <property type="entry name" value="asd_EA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006416">
        <property type="entry name" value="PRK08664.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46718">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46718:SF1">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000148">
        <property type="entry name" value="ASA_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01103">
        <property type="entry name" value="ASD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Semialdehyde dehydrogenase NAD-binding" evidence="11">
        <location>
            <begin position="5"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="10">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000148-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH34790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="358" mass="39535" checksum="02231EE4BDA666BA" modified="2018-07-18" version="1">MAKKRVAIIGVTGSVGQEFVQSLNNHPWFEVTQIAASERSAGKKYLDAIKDSNGIVAWDVGGEIPEYIKEMTVKSINELDVSQLDLVFSAVESEAARDIETKMAVDLPVISTSSAYRYENDVPILIPGINDEQTELLDIQKKNRNWKGWVAPLPNCTTTGLAITLKPLLEKYGAKKVMMTSMQAISGGGKSGVSAMGITDNIIPYIPKEEGKVRQETRKILGKLKDGKIEEADIRISCTCTRVPVIDGHTESVFVETTKEIDPARAKETYNQCNKDISVIGLPSAPKDYYAFHEDPTRPQPRMERTVGDGMTTTIGRVEKEELFDNGLKYMLFSHNKKMGSAKGAVLLAEMLYKKGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSZ4</accession>
    <name>A0A2S2KSZ4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chaperone protein DnaJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877183.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSZ4"/>
    <dbReference type="GeneID" id="76209130"/>
    <dbReference type="OrthoDB" id="5596at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="87" mass="9457" checksum="1AB48EFC8403084D" modified="2018-07-18" version="1">MTEDVCDFCKGIGSSGPNVCVYCNGTGEWNNAAQAYVKNHICQCIVLDRKFCPVCNKACHHDTSLNPKQKIDPGYGGMSSREITVIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT24</accession>
    <name>A0A2S2KT24_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator AsnC/Lrp ligand binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_15830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT24"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF8">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR PTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="1">
        <location>
            <begin position="36"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="120" mass="13921" checksum="5466EFF883C13C3F" modified="2018-07-18" version="1">MVYSRIKRLVKRKLIERFTIDVNDAELGYNVKALTGINMDTKNRDHIIEELFKIDGVREVAEVTGRFDILVTMYSKSLDQMHKMISERIGRIEGIQSSESFIEMKSRMKAMPYMPSKGSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT28</accession>
    <name>A0A2S2KT28_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">threonine-phosphate decarboxylase</fullName>
            <ecNumber evidence="4">4.1.1.81</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">L-threonine-O-3-phosphate decarboxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">NZNM25_15800</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Decarboxylates L-threonine-O-3-phosphate to yield (R)-1-amino-2-propanol O-2-phosphate, the precursor for the linkage between the nucleotide loop and the corrin ring in cobalamin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>O-phospho-L-threonine + H(+) = (R)-1-aminopropan-2-yl phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:11492"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58563"/>
            <dbReference type="ChEBI" id="CHEBI:58675"/>
            <dbReference type="EC" id="4.1.1.81"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.81" evidence="4"/>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877408.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT28"/>
    <dbReference type="GeneID" id="76208407"/>
    <dbReference type="OrthoDB" id="39225at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048472">
        <property type="term" value="F:threonine-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005860">
        <property type="entry name" value="CobD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01140">
        <property type="entry name" value="L_thr_O3P_dcar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42885">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42885:SF1">
        <property type="entry name" value="THREONINE-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="10">
        <location>
            <begin position="29"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="359" mass="40848" checksum="207B275462172DA5" modified="2018-07-18" version="1">MKIRTKSSIIRHIPVIHGGRHSLKNSNPQILDFSSNINPLGIPPSVKNFLKKNLDTIQNYPDLDSSELILSIKKYTQLDKSNILVGNGAIELIYNFCFAFLSGKKVLIPVPTFQEYETAAKLNNSKISFFKTLDLSENINSFISKIPRNGCIFICNPNNPTGKLLPKKQMLKIIQKAQKNSSLVFVDECFIELVPESNESVISYIKKYDNLFVLRSLTKSFGFPGLRIGYAVASKQIVEILQKIKIPWSVNSLAQHAAKISLNNKSHITKSKLVIKKESNFLKNKINQFYGFECCDSSTNFILIKTIHDSTELQKKLLKHKILIRDCKNFRGLDNHYFRIAVKSHKDNIKLVKALENFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT99</accession>
    <name>A0A2S2KT99_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_16910</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829106.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT99"/>
    <dbReference type="GeneID" id="76208295"/>
    <dbReference type="OrthoDB" id="3145at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="57" mass="6926" checksum="846079CB117A6357" modified="2018-07-18" version="1">MKNSELKKLISQYKELEEKKGKKYANNFKISETLKIIEHRYFHETGRKLKSDLRELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTA9</accession>
    <name>A0A2S2KTA9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_17010</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877520.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTA9"/>
    <dbReference type="GeneID" id="76208285"/>
    <dbReference type="OrthoDB" id="35685at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008453">
        <property type="term" value="F:alanine-glyoxylate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004760">
        <property type="term" value="F:L-serine-pyruvate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019265">
        <property type="term" value="P:glycine biosynthetic process, by transamination of glyoxylate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000250">
        <property type="entry name" value="Aminotransferase, class V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024169">
        <property type="entry name" value="SP_NH2Trfase/AEP_transaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152:SF24">
        <property type="entry name" value="ALANINE--GLYOXYLATE AMINOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152">
        <property type="entry name" value="AMINOTRANSFERASE CLASS V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000524">
        <property type="entry name" value="SPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="6">
        <location>
            <begin position="26"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="381" mass="41810" checksum="50A18CD8290E67E9" modified="2018-07-18" version="1">MEYLSMLPGPTNVPNRVMRAMLAPIINHRSDDFVELYTDVVEKTQQVFETQNDIVALSASGTGAVEAGVVNLVKKGDKIIIPVNGEFSNRLAQLIEGQGANVVKLQTPPGENATFDQVKEAFDNNKDVKAFYVVHNETSTGTMVNYLDKVSDLTSRNDAFYVVDSVSLLGGAQLPVDKWNIDVCMTGAQKAIAAPPGISPISVSAKAKKYMIENPPPTMYFNLARYFKYYDEEKHTPFTPALPLLYAYREALSIMLEEGLQNVFNRHKICSDALYSGLSAMGLTPFAKEEDRSISIVALNYLEGLEDKTFRNTLANKFKVLVAGGFGNLKGKVFRVGCMGEVSPYHVMRTISAISSTLAMMGYDVDAQAGLKTAEEKLKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTB5</accession>
    <name>A0A2S2KTB5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_16670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877492.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTB5"/>
    <dbReference type="GeneID" id="76208318"/>
    <dbReference type="OrthoDB" id="11280at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="78" mass="8792" checksum="65B3CB276C8AF324" modified="2018-07-18" version="1">MFNFPKKKTEISTEVLIRFIWVSSFLAMIFTLPPLGLFLGIYYFTGELIIGAVIGFGVHFVILAFSGRISKIITKIMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTB9</accession>
    <name>A0A2S2KTB9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cell division protein FtsX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">lolC</name>
        <name evidence="9" type="ORF">NZNM25_16830</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTB9"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098797">
        <property type="term" value="C:plasma membrane protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044874">
        <property type="term" value="P:lipoprotein localization to outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051447">
        <property type="entry name" value="Lipoprotein-release_system"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30489">
        <property type="entry name" value="LIPOPROTEIN-RELEASING SYSTEM TRANSMEMBRANE PROTEIN LOLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30489:SF0">
        <property type="entry name" value="LIPOPROTEIN-RELEASING SYSTEM TRANSMEMBRANE PROTEIN LOLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="358"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="MacB-like periplasmic core" evidence="8">
        <location>
            <begin position="22"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="ABC3 transporter permease C-terminal" evidence="7">
        <location>
            <begin position="268"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="395" mass="43477" checksum="7ADAF5B9F12175F0" modified="2018-07-18" version="1">MEYRLRLAKRMLFNKKGSLIGAVLAVTIGILVIHVNFVIFQGLFDAIVRDISDYRNGDILVTDDADFIDKSDLSLVNWFERIPYVEAATPRLSSTAEMNMTKSGKLVEETRVPIVGVDPFRDILASTVAQTITDGSYVFSRNSIVLGSNVARDLGGAQVGDSVKVLIVDRYGQDQIRRFTVSGIAQSPGGQGFDYTAVVHIDTLRDMMNRHGDTGSLIVKLNDPSKAFEVKEFFLRSFPNDDFIAETIEESAEQQLAGFRSGIAMINMIGYFGMMSSAFAIVTIQMMLVNGKTREIGVMRAIGAKRKDILIIFIFQGMIIGAIGAGVGTAAGLGYTFYAKETKMSFDNSLPLEVSYNWEKIIQTALTSFVLAIIASLYPSYRATKLLPVEAMRTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTC0</accession>
    <name>A0A2S2KTC0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_16700</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877495.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTC0"/>
    <dbReference type="GeneID" id="76208315"/>
    <dbReference type="OrthoDB" id="8555at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="118" mass="13355" checksum="0FAF8C3E738875C7" modified="2018-07-18" version="1">MQYFQAVQQGKLRASKSQMKMFDVAGFAMLTLTTKKIDGKFHPVGEEEFTAVINSPDGYVAVIVDKDGFTKAQSKALKKDEAISIFKKLLDAGMQEYPEKEIQIWSETRQTIQNEKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTC6</accession>
    <name>A0A2S2KTC6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_15060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877300.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTC6"/>
    <dbReference type="GeneID" id="76208990"/>
    <dbReference type="OrthoDB" id="380802at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="128" mass="13302" checksum="E96FBBD88085D8D6" modified="2018-07-18" version="1">MGLFLLAVLLFSTFGVVLLSTQGLANPLDTPSNVSKMGSLQVFDSSDSTLLGLNLGTSDAISSVTLTFDSNIPEDTTVTISLKDANDVEIGTGSTVTGSPTQTVVISLSDSITDPERRTLQYASITIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTD6</accession>
    <name>A0A2S2KTD6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_15160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTD6"/>
    <dbReference type="OrthoDB" id="10079at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="252" mass="29099" checksum="A293428D46AAFD5A" modified="2018-07-18" version="1">MPVSRKIMICSILIMLSVIIILKTGSSEALNTSNDFDEYRISQMPETRFFDQYDELSAPEKTAVVYPILTQTAYAWGGIHDFYLGRCDTCSEIKIEEYYDPIFSVGAKSFRILEFLGYSVIDDIDIDKNPEILDNFNSIVLLHNEFVTENEFLAITSHPNVIFMYPGALNSKVKINYDKETMILQRGPSFPDPDTVNGFDWKYDNSNISNNTICEEWEFYKIENGHMLNCTPEDIIQNNDKILKELKRLAEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTG2</accession>
    <name>A0A2S2KTG2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribonuclease Z</fullName>
            <shortName evidence="9">RNase Z</shortName>
            <ecNumber evidence="9">3.1.26.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">tRNA 3 endonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">tRNase Z</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 11" type="primary">rnz</name>
        <name evidence="11" type="ORF">NZNM25_17510</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.</text>
            <dbReference type="EC" id="3.1.26.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 9">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the RNase Z family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.11" evidence="9"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877559.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTG2"/>
    <dbReference type="GeneID" id="76208234"/>
    <dbReference type="OrthoDB" id="85118at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042781">
        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07717">
        <property type="entry name" value="RNaseZ_ZiPD-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000002">
        <property type="entry name" value="Ribonuclease Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01818">
        <property type="entry name" value="RNase_Z_BN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013471">
        <property type="entry name" value="RNase_Z/BN"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000801">
        <property type="entry name" value="PRK00055.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02651">
        <property type="entry name" value="RNase_Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46018">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46018:SF2">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23023">
        <property type="entry name" value="Anti-Pycsar_Apyc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="8 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="10">
        <location>
            <begin position="197"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="299" mass="33624" checksum="390A863B1154596A" modified="2018-07-18" version="1">MKLVFLGTSAAQPTENRGLSCICLERDGEILMFDAGEAAQISYMKSGLGWNKKMKVFVTHLHGDHCVGILGLLQTMSMQNRTEILEIFGPNGIEEFIAANIKVLNFGLSFPVLITIIKEGKVFEDKKFSIYTCKANHSITAFSYLFEEKDKPGRFNIDEAKKLGIPEGDLWHKLQNGHEIEINGKTIKPEQVLGEKRPGKKIGISGDTMPTKELELFFENCDYLVFDSTFLDEEKQRAQDTCHSTAKQAATIAKNAKVKNLILTHFSARYKDENGHLEEAKKIHSSVITARDLLEIEIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTG6</accession>
    <name>A0A2S2KTG6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_16620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877487.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTG6"/>
    <dbReference type="GeneID" id="76208323"/>
    <dbReference type="OrthoDB" id="10789at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="264" mass="30811" checksum="6FD18B2EC453EC78" modified="2018-07-18" version="1">MKNFFYIALPILAFLLIFVNTFFGHFIFSSETQQVINKYSVYVHLLSEWQSDSKNIIFDVTNSWYKSDQVSDEDHVFNAESKEYNTNQLQEIHGKSYVELKHEFSECHEEWQPMLYRKAIDTVRHEIEYVQGKQLSTDPGISVYPDIENRNYDNSEQQSKIKSGYIQFVPICTSKEITSYDYSVKIDNKDLGFDVYFVSSSKQRENFGSSDFDSYTELGCFGQNKQSYSGTCKNIKKDGGLLLVFPDELKPWTTKVTINLYEIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTG8</accession>
    <name>A0A2S2KTG8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dUTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_17170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTG8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11527">
        <property type="entry name" value="NTP-PPase_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4010.10">
        <property type="entry name" value="Type II deoxyuridine triphosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014871">
        <property type="entry name" value="dUTPase/dCTP_pyrophosphatase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08761">
        <property type="entry name" value="dUTPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101386">
        <property type="entry name" value="all-alpha NTP pyrophosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="13737" checksum="9EBE885AEE62A7F5" modified="2018-07-18" version="1">MTQEMSEDRLDTIFKLQKGLSEMMNLDRYPKDSEGRVSALCTAIMHEAVELQRTTNWKWWKTPTQFNEAEAREELIDIWHFVVQASLELNLTPDDIVDEYKKKNEINRERQRNGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTI2</accession>
    <name>A0A2S2KTI2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_17440</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877552.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTI2"/>
    <dbReference type="GeneID" id="76208241"/>
    <dbReference type="OrthoDB" id="43026at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF11">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="315"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="339"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="381"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="403"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="434" mass="46961" checksum="4B1876CD15CCAB4C" modified="2018-07-18" version="1">MSELKRHMGLFHLTMYGVGLILGAGIYVLIGEATGIAGDAVWISFALGSIVALFAGFSYAELSSVFPKAAAEYIFIKNAFKNNFFAFLIGWLTVITSIITAATVALGFGGYFAEFVDIPIIISAIGLLVILSIVNFIGIRESSWTNTIFTIIEASGLILIIIIGFTFSNPEPVNYLESPSGFTGVAIVFVLIFFAFIGFEDMANIAEEVRKPKKTIPKAIILSVLISGTIYILVSLAVVRVVNWEELATSSAPISLVAERGLGSEAHILLSSIALFAITNTVLITLVAGSRMFYGMAREKVFPSILGKIHFKTKTPWIAVVMILIISTLFTLIGDIVIVANITVFAIVITFAAVNLAVIVLRYTEPNVERKFKVPINIGKFPILPLFGLGISIYMGFQFEIEVVLVGLTIIGIGAVFYRISKNSTTNSPNISQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTJ0</accession>
    <name>A0A2S2KTJ0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0201 protein NZNM25_17540</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_17540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0201 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877562.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTJ0"/>
    <dbReference type="GeneID" id="76208231"/>
    <dbReference type="OrthoDB" id="7819at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01112">
        <property type="entry name" value="UPF0201"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002739">
        <property type="entry name" value="PAB1135-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39652">
        <property type="entry name" value="UPF0201 PROTEIN TK1335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39652:SF1">
        <property type="entry name" value="UPF0201 PROTEIN TK1335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01877">
        <property type="entry name" value="RNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="132" mass="15173" checksum="41196EA7B935E3FE" modified="2018-07-18" version="1">MKTPDINCKIQMITPLNSSENSEKVKKAISNIFPYSTINSDSFTISAQSKELRSFEKIYEVIHSKKSQNTYRRYLERNLDNNTTWFYLNKQAAFVEQIAICEEEGESPLGPIKVIIASSNIDEIIDWIVFGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTP9</accession>
    <name>A0A2S2KTP9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">DNA-directed RNA polymerase subunit M</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_17280</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877541.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTP9"/>
    <dbReference type="GeneID" id="76208257"/>
    <dbReference type="OrthoDB" id="72957at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10511">
        <property type="entry name" value="Zn-ribbon_TFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006288">
        <property type="entry name" value="TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01384">
        <property type="entry name" value="TFS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF12">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 6 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="8">
        <location>
            <begin position="62"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="6">
        <location>
            <begin position="4"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="4"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="105" mass="12249" checksum="8AA4C82CE19F67D2" modified="2018-07-18" version="1">MKFCPKCEVKLKKGTLGLECSKCGYIEGQKAKETKKIIDEDEPDFSLLAFEGNEGEDTNPTIKLECEKCGHDEAVWWMFQTRSADEPTTRFYRCQKCKYTWRDYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTQ1</accession>
    <name>A0A2S2KTQ1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">7-carboxy-7-deazaguanine synthase</fullName>
            <shortName evidence="8">CDG synthase</shortName>
            <ecNumber evidence="8">4.3.99.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Archaeosine biosynthesis protein QueE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">queE</name>
        <name evidence="10" type="ORF">NZNM25_18130</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>6-carboxy-5,6,7,8-tetrahydropterin + H(+) = 7-carboxy-7-carbaguanine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:27974"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:61032"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="EC" id="4.3.99.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </cofactor>
        <text evidence="8">Binds 1 S-adenosyl-L-methionine per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="8">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the radical SAM superfamily. 7-carboxy-7-deazaguanine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.99.3" evidence="8"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877614.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTQ1"/>
    <dbReference type="GeneID" id="76210308"/>
    <dbReference type="OrthoDB" id="7980at2157"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016840">
        <property type="term" value="F:carbon-nitrogen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00917">
        <property type="entry name" value="QueE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024924">
        <property type="entry name" value="7-CO-7-deazaguanine_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42836">
        <property type="entry name" value="7-CARBOXY-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42836:SF1">
        <property type="entry name" value="7-CARBOXY-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13353">
        <property type="entry name" value="Fer4_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5 8" id="KW-0408">Iron</keyword>
    <keyword evidence="6 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="9">
        <location>
            <begin position="18"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="12"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="37"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH35022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="237" mass="27145" checksum="CAADB87AD8506A57" modified="2018-07-18" version="1">MKVRLFEIFTSVEGEGILYGTKTLFIRLAGCPFTCFYCDTKESLPLDSGTEYAIEEANRLIDSNLQRQTYKVNFTGGDPLIQHQAVALLAKHVQDKKIPTYLESSCFDIDRFNHVLPFIDIVKIEFKTKDSDFVDSDHYEKLIDNTMKCLKSSIEAKKITYIKIVVSSKTKLDEFKELVNQIFKIVSKDDIDGFIIQPTYGISEPSLDLLLNLYDVVYPYYIDVKVVPQLHKFIGAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTW8</accession>
    <name>A0A2S2KTW8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Transcription initiation factor IIB</fullName>
            <shortName evidence="7">TFIIB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tfb_10</name>
        <name evidence="7" type="synonym">tfb</name>
        <name evidence="10" type="ORF">NZNM25_17980</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the TFIIB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877601.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTW8"/>
    <dbReference type="GeneID" id="76210323"/>
    <dbReference type="OrthoDB" id="7429at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20550">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000023">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00383">
        <property type="entry name" value="TF2B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023484">
        <property type="entry name" value="TFIIB_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="9">
        <location>
            <begin position="5"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="repeat" description="1" evidence="7">
        <location>
            <begin position="122"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="repeat" description="2" evidence="7">
        <location>
            <begin position="216"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH35007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="301" mass="33293" checksum="753629568FA50728" modified="2018-07-18" version="1">MVSKGKDLCPRCAQGKLVTDNESGEMFCSKCGFVITEKLQEAGPEWRSFTQDEGGNKARAGAPTSLTMHDMGLATIINPINKDASGRPLTASMKSTIERLRTWDSRSQVHEPVDRNFRQAFSELNRLKDKLAISDAVIEKAAYIYRKALEKGLVRGRSISALMASALYAACRDTETPRNLKDVEQAANIKRKDIARCYRLLVKELDLKMPVTDSIQCVARIASRIGIAEKTKRYATKVLKMAQDNEVSAGKDPMGLAAAALYLSCVKNGEDKTQRDIAEAANVTEVTIRNRYKGLKESLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTY0</accession>
    <name>A0A2S2KTY0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_17090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877527.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTY0"/>
    <dbReference type="GeneID" id="76208275"/>
    <dbReference type="OrthoDB" id="8386at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="76" mass="8720" checksum="E4CFD807955C68F0" modified="2018-07-18" version="1">MNRTNQSTIIKEAINSYLDKGVTDKQDIYTKVVDELGVPRPTVRRVARDLRTELLQKIQILQSDMPKVSTTSEEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTZ2</accession>
    <name>A0A2S2KTZ2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein NZNM25_18900</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_18900</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877712.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTZ2"/>
    <dbReference type="GeneID" id="76210117"/>
    <dbReference type="OrthoDB" id="25187at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.700.20">
        <property type="entry name" value="Hypothetical protein ph0010, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.150">
        <property type="entry name" value="Hypothetical protein ph0010, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00645">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023473">
        <property type="entry name" value="AMMECR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036071">
        <property type="entry name" value="AMMECR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002733">
        <property type="entry name" value="AMMECR1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027485">
        <property type="entry name" value="AMMECR1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027623">
        <property type="entry name" value="AmmeMemoSam_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023472">
        <property type="entry name" value="Uncharacterised_MJ0810"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04335">
        <property type="entry name" value="AmmeMemoSam_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00296">
        <property type="entry name" value="TIGR00296 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016:SF0">
        <property type="entry name" value="AMME SYNDROME CANDIDATE GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016">
        <property type="entry name" value="AMMECR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01871">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143447">
        <property type="entry name" value="AMMECR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51112">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMMECR1" evidence="2">
        <location>
            <begin position="10"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="206" mass="23084" checksum="A84B099262DD0121" modified="2018-07-18" version="1">MKNIRNFTDEDGKELVKTARKAVAEYLKNKSKIADSDFHSRFNFESGVFVTINKKDSLRGCIGYPIPIKKLSYGLIDAAISAATQDPRFTPVSTDELDKIVFEVTVLTPPVEIKVDDPLEYLSIIKVGRDGLIVENSYSSGLLLPQVPTEYGWDVEEFLQHTCEKAGINKDSWKEGSTKISIFEGVIFKEESPNGKIVRESTNNFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTZ5</accession>
    <name>A0A2S2KTZ5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminotransferase DegT</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_17190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DegT/DnrJ/EryC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829109.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTZ5"/>
    <dbReference type="GeneID" id="76208266"/>
    <dbReference type="OrthoDB" id="10355at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00616">
        <property type="entry name" value="AHBA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000653">
        <property type="entry name" value="DegT/StrS_aminotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30244:SF34">
        <property type="entry name" value="DTDP-4-AMINO-4,6-DIDEOXYGALACTOSE TRANSAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30244">
        <property type="entry name" value="TRANSAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01041">
        <property type="entry name" value="DegT_DnrJ_EryC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000390">
        <property type="entry name" value="PLP_StrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="360" mass="39882" checksum="969B2B732CF5E2BE" modified="2018-07-18" version="1">MKIAINIPIVGKEEISAVTNILKNGALTSSASQGGKHVQDFERTAASFVKSKYAIAVNSGTAALQAALHALDIKKGDEVLVPSFTFVATANAVISTGAKPIFVDILKENYTINPDDLEKKINKKTRAIIPVHLYGNAAHVERLSEISKKHNLPIIEDSAQSLGTTYKGKHTGTFFEMGCYSMYPAKVMTSGEGGFVVTNNKKLRDKLLMIRNHGMVHGYDTRIFGLNLRLPEINAAIATVQMKKLPNFLKSRKKNAELLSKLISDLKLVLPKQRKNENVNWYLYTIAIEKRDKILKKLNEKGIGAASYYPTPVHKTPFYKLKTKLPVTDWAASHVLSLPIHPGVTQKNIEFIAKILHEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU10</accession>
    <name>A0A2S2KU10_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_18620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877686.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU10"/>
    <dbReference type="GeneID" id="76210090"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="70" mass="8507" checksum="3CD60AD89F817EA3" modified="2018-07-18" version="1">MENQKSPIFEYDERYLATSKHTRKELYKLQEIVLEQIKTNPSTYTYELKKIKDYDQKEIDTILTMLTTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU20</accession>
    <name>A0A2S2KU20_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Large ribosomal subunit protein eL24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rpl24e</name>
        <name evidence="12" type="ORF">NZNM25_18480</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9 10">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L14.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877673.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU20"/>
    <dbReference type="GeneID" id="76210073"/>
    <dbReference type="OrthoDB" id="55506at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00472">
        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.170.20:FF:000001">
        <property type="entry name" value="probable ribosome biogenesis protein RLP24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="entry name" value="Ribosomal protein L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00773">
        <property type="entry name" value="Ribosomal_eL24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038630">
        <property type="entry name" value="L24e/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056366">
        <property type="entry name" value="Ribosomal_eL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055345">
        <property type="entry name" value="Ribosomal_eL24-rel_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_eL24-rel_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF034186">
        <property type="entry name" value="PRK14891.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 10 12" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 10" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="5 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TRASH" evidence="11">
        <location>
            <begin position="8"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="10">
        <location>
            <begin position="8"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH35057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="66" mass="7548" checksum="C42F6569EDF419CF" modified="2018-07-18" version="1">MSLLVKPCNFCDRPVAKGSGTMLAKNDGTVLWFCSSKCKKNMLDLKRDPRKLKWTKKYVKGGIRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU61</accession>
    <name>A0A2S2KU61_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UDP-N-acetylglucosamine 2-epimerase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_19470</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877757.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU61"/>
    <dbReference type="GeneID" id="76210177"/>
    <dbReference type="OrthoDB" id="3302at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="628" mass="73625" checksum="B3CF613189807FF1" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU89</accession>
    <name>A0A2S2KU89_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="16">tRNA lysidine(34) synthetase TilS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">NZNM25_19900</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877796.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU89"/>
    <dbReference type="GeneID" id="76210220"/>
    <dbReference type="OrthoDB" id="33422at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002144">
        <property type="term" value="C:cytosolic tRNA wobble base thiouridylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000174">
        <property type="entry name" value="ATPase, PP-loop superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000541">
        <property type="entry name" value="Ncs6/Tuc1/Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054306">
        <property type="entry name" value="TtuA-like_LIM_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00269">
        <property type="entry name" value="TIGR00269 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807">
        <property type="entry name" value="ATPASES OF THE PP SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807:SF12">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22082">
        <property type="entry name" value="TtuA_LIM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="2-thiouridine synthetase TtuA-like N-terminal LIM" evidence="15">
        <location>
            <begin position="3"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal" evidence="14">
        <location>
            <begin position="50"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="3"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="53"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR004976-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR004976-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF22082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GBH35199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="304" mass="34250" checksum="9CBC542EC0F48794" modified="2018-07-18" version="1">MICDKCENPAVYTRKYSGQKLCSVCFSNSIVRKTAKTISKHNMIKYNELVAVAVSGGKDSLALLKIIHEMALTHNFRIIAVTIDEGIPGYRNEALEIVEKFCEKLNVKHKVYSYKDLFELTLDEALELRENEKTSSCSICGILRRRAIDFAAKDVGADVIATGHNLDDTLQTFIINMLSGDTTKIGWMDPDTSSNSLRKIKPFCEIYESEIVFYAFTNDIPFQSEPCPHMNEGIRTDIREFLNSLENQHSGIKNNLYQSILKVSQSVKTLNSKEKTVCEKCGTECTGKICSVCNMVLKLKENQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUB3</accession>
    <name>A0A2S2KUB3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_19720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877781.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUB3"/>
    <dbReference type="GeneID" id="76210202"/>
    <dbReference type="OrthoDB" id="3111at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051450">
        <property type="entry name" value="Gfo/Idh/MocA_Oxidoreductases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055170">
        <property type="entry name" value="GFO_IDH_MocA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377:SF1">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22725">
        <property type="entry name" value="GFO_IDH_MocA_C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="GFO/IDH/MocA-like oxidoreductase" evidence="2">
        <location>
            <begin position="123"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="327" mass="37459" checksum="B9D7F484083E4BD6" modified="2018-07-18" version="1">MKIAVIGYGSIGKRHVQNLLSIPDTEIIICTKQKIKNPSTKNVKIVKSISDCIKEKPKVGIIANESSFHIQIAMKLAKSGMDLFIEKPLSNSFFGTKELVRIIKKKKIITQMGCQFRFHRCIKEIKHMISTCKLGKVISVSAECGSFLPEWHPHEDYTKSYAAREDLGGGVVLTNIHEIDYLYWFFGDVSKVFSITGKFSELKISADDLCVGILQFKNKVIAELHLDYFQKPDFRSCKIIGTKGTVIWDSDTNNVMFYDNKKNKWVKILKWTRYDRNSMFKEEIIHFLHCVKKRETTINPVEKDGVNTLKIALSIIKSSKSRRVVKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUC3</accession>
    <name>A0A2S2KUC3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_19480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877758.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUC3"/>
    <dbReference type="GeneID" id="76210178"/>
    <dbReference type="OrthoDB" id="147504at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053173">
        <property type="entry name" value="SAM-binding_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45128">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45128:SF1">
        <property type="entry name" value="S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE RV2258C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="1">
        <location>
            <begin position="50"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="334" mass="38545" checksum="9858409A1C457475" modified="2018-07-18" version="1">MTKDDTITQKVENMYKKYPYPSPSTDAAQTNELLNLLRIFEMESKIKLEEKNILDAGSGSGHRITNVAQFFKKCNFLGIDISEKSLEIANELKNKKNTKNIQFQKHNIMTGVENLGKFDIVLCMGVLHHLSNPSKGLQMLTKTLKDDGIIFLYLYGKLGGHKRMLNKEMISILLGKEKSNYDLGIELVRDLELNKFDYGWNLNFKNKKEEDTLIVDSLLHTNEFLYDFNDIDKLFKKTNLYGYSIFGITESTQGLLFDSSIKTEKKLSIPQTNVSQKLKSNLSLSFYESLNLKEKFRIIDLLYEPNGYTLVGFTKYAYDKLSNDRIKRNFIVIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUI0</accession>
    <name>A0A2S2KUI0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Aspartate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_18990</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aspartokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877717.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUI0"/>
    <dbReference type="GeneID" id="76210125"/>
    <dbReference type="OrthoDB" id="8904at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004072">
        <property type="term" value="F:aspartate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009090">
        <property type="term" value="P:homoserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1320">
        <property type="entry name" value="Aspartokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042199">
        <property type="entry name" value="AsparK_Bifunc_asparK/hSer_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21499">
        <property type="entry name" value="ASPARTATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21499:SF59">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="2">
        <location>
            <begin position="405"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="471" mass="52373" checksum="12F45ADC221E2238" modified="2018-07-18" version="1">MTKLVVAKFGGSAIGPDGMLIPEIINRINNLKKDSKVIAVFSAPLTTHNGKTRSLTDVILEQGKNAENGQSPSLDIVKSTYDKILQLVNPENMDACKNTIDLNLKKASKALDDALNAKEFVDEVRSRALAFSGEILMSHVMNYVLKSNGIKSDSVDFDDWPIITDNNIESTNFLASKSRENMDKIALLVEQNEVVTMGGFIGKTIKNVTTTYERGGSDRTAADLGILFHKIYETSIDFEKDSAVVSADPKIVESNLSQVLQLSYNEARLAGMFGMKILDPIAIKEIVENGVDIPIIITNMNDPEKTTTIKRILDEQKGHPIKIVTGKENCAIFRIETNLIQKLLTSLEKDKRYSEFVMLSPFTKDGIEFSRILFLDGDYVKRNEKYLLGFDSLATITYNRGVITLIGDEMWRVQQVASRTSAKIGDAGLNILNMDAQEETSRIIIVVEDADDNIRKAIKAIHQEISKINFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNQ6</accession>
    <name>A0A2S2KNQ6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl23</name>
        <name evidence="5" type="ORF">NZNM25_00230</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds to 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit. Contacts protein L29.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875905.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNQ6"/>
    <dbReference type="GeneID" id="76209349"/>
    <dbReference type="OrthoDB" id="7751at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_A">
        <property type="entry name" value="Ribosomal_uL23_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_uL23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011118">
        <property type="entry name" value="PRK14548.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L23A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="88" mass="9959" checksum="5872DBDD99459206" modified="2018-07-18" version="1">MNVDQANKIILKPYITEKTFAMVENESKICFIVERSASKPEIAEAVKALYKENVTNVNTARTIYGKKAFVQFENTEKARDLATKIGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNS5</accession>
    <name>A0A2S2KNS5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphoribosylformylglycinamidine synthase subunit PurS</fullName>
            <shortName evidence="6">FGAM synthase</shortName>
            <ecNumber evidence="6">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Formylglycinamide ribonucleotide amidotransferase subunit III</fullName>
            <shortName evidence="6">FGAR amidotransferase III</shortName>
            <shortName evidence="6">FGAR-AT III</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Phosphoribosylformylglycinamidine synthase subunit III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">purS</name>
        <name evidence="7" type="ORF">NZNM25_00530</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PurS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875933.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNS5"/>
    <dbReference type="GeneID" id="76209382"/>
    <dbReference type="OrthoDB" id="56303at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1280.10">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase subunit PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01926">
        <property type="entry name" value="PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003850">
        <property type="entry name" value="PurS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036604">
        <property type="entry name" value="PurS-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00302">
        <property type="entry name" value="phosphoribosylformylglycinamidine synthase subunit PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34696">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34696:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02700">
        <property type="entry name" value="PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82697">
        <property type="entry name" value="PurS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="86" mass="9692" checksum="042CC4D98191ACBB" modified="2018-07-18" version="1">MPIFDVHVTIENKPGMSDPEGDTILNDLVLKGTHKSVSKIKTAKMLKFTIKEKDKKSAQSKVQEICDELRIYNPMVSKVTIDVFEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNT0</accession>
    <name>A0A2S2KNT0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00700</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875947.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNT0"/>
    <dbReference type="GeneID" id="76209399"/>
    <dbReference type="OrthoDB" id="11522at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="77" mass="8853" checksum="497C54F255EB7351" modified="2018-07-18" version="1">MGIKSPSEYVDFFINLNMGEDVSLLSFISNEKNILKKNLELKNINKEPIKKGIEILELLVREINENGEKTVLGKYQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNT7</accession>
    <name>A0A2S2KNT7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CxxC-x17-CxxC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_01210</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875995.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNT7"/>
    <dbReference type="GeneID" id="76209450"/>
    <dbReference type="OrthoDB" id="144439at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026363">
        <property type="entry name" value="CxxC-x17-CxxC_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04272">
        <property type="entry name" value="cxxc_cxxc_Mbark"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23477">
        <property type="entry name" value="zf_Tbcl_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxxC-x17-CxxC" evidence="2">
        <location>
            <begin position="7"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="CxxC-x17-CxxC" evidence="2">
        <location>
            <begin position="75"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="113" mass="13037" checksum="0F83A0459F51E0EF" modified="2018-07-18" version="1">MSFDREREMFTAKCGDCGNECQVPFKPKDDRPVYCRECFQNHKPAPRPGGRFGGRSGGYGGDRGSRFGRRDDRPREMFDAKCGDCGNDCQVPFRPKDDRPVYCRECFQNHKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNV9</accession>
    <name>A0A2S2KNV9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Arsenate reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNV9"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006660">
        <property type="entry name" value="Arsenate_reductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30041">
        <property type="entry name" value="ARSENATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30041:SF4">
        <property type="entry name" value="ARSENATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03960">
        <property type="entry name" value="ArsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51353">
        <property type="entry name" value="ARSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="90" mass="10382" checksum="9BB5F476D66042C9" modified="2018-07-18" version="1">MKIDIEKRDFFKEPLSEGELKKIIKMTGKKPSELLRKKDKMFKELDLENGKKTDSQIIKLMAKYPGLILRPIVIAGNKAFVGKIDSKNFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP73</accession>
    <name>A0A2S2KP73_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ergothioneine biosynthesis protein EgtB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_02330</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; ergothioneine biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876096.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP73"/>
    <dbReference type="GeneID" id="76209567"/>
    <dbReference type="OrthoDB" id="136349at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052699">
        <property type="term" value="P:ergothioneine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.450">
        <property type="entry name" value="dinb family like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1580.10">
        <property type="entry name" value="paralog of FGE (formylglycine-generating enzyme)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024775">
        <property type="entry name" value="DinB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017806">
        <property type="entry name" value="EgtB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051043">
        <property type="entry name" value="Sulfatase_Mod_Factor_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005532">
        <property type="entry name" value="SUMF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042095">
        <property type="entry name" value="SUMF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03440">
        <property type="entry name" value="egtB_TIGR03440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23150:SF36">
        <property type="entry name" value="HERCYNINE OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23150">
        <property type="entry name" value="SULFATASE MODIFYING FACTOR 1, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12867">
        <property type="entry name" value="DinB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03781">
        <property type="entry name" value="FGE-sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="DinB/YfiT-like putative metalloenzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DinB-like" evidence="5">
        <location>
            <begin position="16"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase-modifying factor enzyme-like" evidence="4">
        <location>
            <begin position="178"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="436" mass="51774" checksum="C3D540CF0522449C" modified="2018-07-18" version="1">MASSQKLDQKGVLLEQFKETRNRTLELVKTLEKDDFVVQTAVFMSPPKWHIGHVSWIYEAIMSKLDKSYEFYSKEFTEYLNSYYQQFGVPHDKGLRGVISRPTVDQLFQYFNTINQRVEKFIKSQALDENAIKVITMGFHHECQHQELLVYDLQHLLAEQYRPVKKEIADKPKENDKKSIYIKGGIYEMGYNGKNFCYDIELPEHKVYLNDFKMDIFPVSNQQYLEFIEDGGYENYKYWLSDGWEKVKENKWRSPMYWEKINGEWNVRDFIGIRKINPNEPVCHVSYYEADAYCKWAGKRLPTEAEWEKAACWNEEKQEKSIYPWGNEEPEKDKCNLLESYYWKCTELGTFPNGTSPSGCQQMIGDVWEWTSSEFIGYPGFKSGFDEYNDKWFTNQKVLRGGSFATPKMSIRGSYRNFFRLDERWLFSGFRCAEDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP85</accession>
    <name>A0A2S2KP85_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">SAM-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_02570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP85"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861:SF1">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="225" mass="26666" checksum="075CC055878A77FC" modified="2018-07-18" version="1">MEIKKQKSMDKVRKTFDEWAQNGRAELMEIEHGKNVVKFLQTISFDKPFTFLDVGCGNGWVVRKIVKEKNCKKAVGIDKSKKMIIQATRKKENNKEEYIHTDIETMNNRRKFDFIFSMESLYYANSMELALEKIFKLLKPGGKFFCGTDFYSDNKATAKWAKIMKIQMHLHSKKEWKEFFKNTGFIVKTKQIKDLRNTKKWKREFGTLFIIGTKPKRQYSNSIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP91</accession>
    <name>A0A2S2KP91_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator AsnC/Lrp ligand binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_01320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876003.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP91"/>
    <dbReference type="GeneID" id="76209461"/>
    <dbReference type="OrthoDB" id="6995at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="1">
        <location>
            <begin position="7"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="77" mass="8941" checksum="650E0CF8FA20D7F4" modified="2018-07-18" version="1">MTDAYVMLNCELGAESQILEELRQIEQVVDVFETIGTHDMLVKIQAENFEKIREIVSWNIQKMKNVRSTATLIKKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPC2</accession>
    <name>A0A2S2KPC2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_02080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876076.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPC2"/>
    <dbReference type="GeneID" id="76209542"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="145" mass="16828" checksum="8C0539D30CE635C6" modified="2018-07-18" version="1">MQTRLLILVALVLIVVPIGFFYYMSINGFSIHHAGIYVQLLSEQQIEHFAVSEPKTITQEELMQFPQISIMINLLLEEKENPQGTRSFFVNFDTYRIFDSRGELKIKNHMSDSGARNLYQDFTQSFESNVVVYEGNYFSITSWIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPL0</accession>
    <name>A0A2S2KPL0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03870</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876232.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPL0"/>
    <dbReference type="GeneID" id="76209729"/>
    <dbReference type="OrthoDB" id="2051at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="112" mass="13166" checksum="1BE6F776EE712198" modified="2018-07-18" version="1">MDNPIDSKKTLEIIEKMRLGKIGQYKKWKPIIKKIKNEEPLNPGELEYYSNLTRIYKNSSISSRSRIYHTKLSELDETPPCNSCGENSEYYCNMNDQYFCTIHIVGHDENEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPL1</accession>
    <name>A0A2S2KPL1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH asnC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_03740</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPL1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF4">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13404">
        <property type="entry name" value="HTH_AsnC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="12"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="169" mass="19241" checksum="F0412207976D798A" modified="2018-07-18" version="1">MLGMSEDAWSNLDKVDQKIIEILNSNARTPSKEIASELKKSGHDVSDRTIRKRIERLEKSGIIKGYKAVLTDVSGINEYQVVLMKIKPSKSLEAVKDSIKDFITKLDNYLLVANMNGEWNMLVIMQLNSEKSNPAQKIVEKFSDELIDYRINEVDIKDVNILNMSLLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPL9</accession>
    <name>A0A2S2KPL9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_02720</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866803.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPL9"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF11">
        <property type="entry name" value="CEREVISIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S8/S53" evidence="7">
        <location>
            <begin position="425"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="7">
        <location>
            <begin position="689"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="76"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="76"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="94"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="434"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="475"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="753"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="810" mass="86614" checksum="03D44195B9712437" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPN8</accession>
    <name>A0A2S2KPN8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphate transport system permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_04170</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Part of the binding-protein-dependent transport system for phosphate; probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876295.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPN8"/>
    <dbReference type="GeneID" id="76209765"/>
    <dbReference type="OrthoDB" id="338493at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011864">
        <property type="entry name" value="Phosphate_PstC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051124">
        <property type="entry name" value="Phosphate_Transport_Permease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02138">
        <property type="entry name" value="phosphate_pstC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30425">
        <property type="entry name" value="PHOSPHATE TRANSPORT SYSTEM PERMEASE PROTEIN PST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30425:SF1">
        <property type="entry name" value="PHOSPHATE TRANSPORT SYSTEM PERMEASE PROTEIN PSTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="78"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="283"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="79"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH33626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="325" mass="34965" checksum="975BE01B8885774C" modified="2018-07-18" version="1">MERINLNPEKHGKRQISDKIFKIGATVAGVYVLVMIALLVFQLVSESYPIWEEDGLSFITSTDWNAVEGRESFGALPYIFGTLVTASLAMLIGVPLSIGIAMFISDAPAKIGGPLGFIVELLAAVPSVIYGLWGLFVFRIYFKDWVEEPLHNLFGDSIFLFSGTPFGLDIITASIILAIMIIPTVSAVSREVMKAVPQQQREAAYMLGATKWEMFKLAVFPYSKTGLIGASILGLGRAVGETMAVTMLIGNATGISAITSSLFKPSQTMSSIIANEFVEASPASLHLPALIGVALILLLIAIAINVVAHLLVTRMMKVKEGAINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPQ5</accession>
    <name>A0A2S2KPQ5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Aquaporin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_04100</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876289.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPQ5"/>
    <dbReference type="GeneID" id="76209757"/>
    <dbReference type="OrthoDB" id="36050at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45724:SF13">
        <property type="entry name" value="AQUAPORIN NIP1-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45724">
        <property type="entry name" value="AQUAPORIN NIP2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="225" mass="24517" checksum="2BC867ADAA3DC9EB" modified="2018-07-18" version="1">MVYSNIQIFTVELIGTFILVIFATGSIVYDVQIGGPYGIWFAAAAPFVALIIGVYSFGKISLAHFNPAVTIGYYITGHISKIQIVWYFAAEIIGAVLGSLFVMTFIGKDANLGSNVPNYDYSLFLIFPVEVLASALLMAVIFTVVYTKGLKGFSGIAIGGIVGLDIFFLAFISGASMNPARSLAPALLSGMMNDLWLYWSAPYVGTIIVAFLFRKKFEIQRKQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPS0</accession>
    <name>A0A2S2KPS0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_04440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876316.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPS0"/>
    <dbReference type="GeneID" id="76209794"/>
    <dbReference type="OrthoDB" id="3327at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052159">
        <property type="entry name" value="Competence_DNA_uptake"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30619">
        <property type="entry name" value="DNA INTERNALIZATION/COMPETENCE PROTEIN COMEC/REC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30619:SF1">
        <property type="entry name" value="RECOMBINATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="92"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="462" mass="52773" checksum="C1D699E0D3A7AA33" modified="2018-07-18" version="1">MSKKDWMFTNQSLTVAYNYTEKYYKQNKSGLPAKRTVVARLLFGEWVKPLSDKNGVPLTAEGQSYVAFRGGVGWIPTDSIGTERTLEIYFLDVGQGDAILIQTPQDNRYLIDGGPTHDEGELAHEFLKWKFNLEGNQIDFDAVIMSHADRDHAMGLVPIVGDQKIKIKKFYHNGISRFRGKPSDGKIMGKNPRRLVEIFDDITKLDFSKISGDYQKLFTALTKAKKKNRDIKIEHLDHTSVIKKLPNDPLEIEILGPINISHNDRVEYLASGTADKMRNGNSVSLMAKYGKCKILLCGDMNTKYEKEFIKFHKDYPLESHVFKANHHGSQDVTTEFLERVRPWISIVSSGDVPDHAHPRAELLGILGKYSSQAIKRPIIFATEILRTYHPVSSEDIEEVKKRKREYSLYERAIDGIVHVRTDGKKLVVGRVYGDPTLTSAKNNPSRTRNQNAYKWEYYEFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPS5</accession>
    <name>A0A2S2KPS5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2-phospho-L-lactate transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_02590</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876119.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPS5"/>
    <dbReference type="GeneID" id="76209594"/>
    <dbReference type="OrthoDB" id="59563at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043743">
        <property type="term" value="F:LPPG:FO 2-phospho-L-lactate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07186">
        <property type="entry name" value="CofD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.240:FF:000001">
        <property type="entry name" value="2-phospho-L-lactate transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.240">
        <property type="entry name" value="CofD-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10680">
        <property type="entry name" value="CofD-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01257">
        <property type="entry name" value="CofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002882">
        <property type="entry name" value="CofD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038136">
        <property type="entry name" value="CofD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010115">
        <property type="entry name" value="FbiA/CofD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01819">
        <property type="entry name" value="F420_cofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43007">
        <property type="entry name" value="2-PHOSPHO-L-LACTATE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43007:SF1">
        <property type="entry name" value="2-PHOSPHO-L-LACTATE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01933">
        <property type="entry name" value="CofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142338">
        <property type="entry name" value="CofD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="306" mass="33928" checksum="931387C210283AF1" modified="2018-07-18" version="1">MITVLAGGTGSVKLVRGLVAQESKVNVISNVGDNYWLYGLYVCPDIDTIVYGLADLLDQERGWGMKKDTFNFLRQMEVFGEETWFRVGDRDAATHLIRTNMLKNGKNLSDITKWMCEKFAVSANIIPVTDNSIETRITTDKGELHLQEYWVKHRGKDPVLGIQYIGADKARPNPEAVNAIHDADMVILAPGNPLTSIGPMLQIKGIRKELSKIKKKVVAVSPLIGDDAVSGPAAKYMQAAGIESNAYGLAKMYSDVCSNIIIDSKDRLLTKKIQSLDMRVFETKITMKNKLAEDALANFILKQVHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPS9</accession>
    <name>A0A2S2KPS9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">4Fe-4S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_04540</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876325.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPS9"/>
    <dbReference type="GeneID" id="76209806"/>
    <dbReference type="OrthoDB" id="23833at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050572">
        <property type="entry name" value="Fe-S_Ferredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687">
        <property type="entry name" value="ADENYLYLSULFATE REDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687:SF6">
        <property type="entry name" value="L-ASPARTATE SEMIALDEHYDE SULFURTRANSFERASE IRON-SULFUR SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="9">
        <location>
            <begin position="247"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="9">
        <location>
            <begin position="284"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="186"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH33663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="315" mass="35415" checksum="E1AA9FC924C0E44C" modified="2018-07-18" version="1">MSLLLKDRVYSMEAPTAKRGVYPLHGYKLGLYRLPIKLEEPAELKSVHDGLKKAFEMDMYADRIYATYRWKEQNMDDVDAKGYEEVELSVTVEIVTGEVVDIIYQIFPIEKFGDPNWVKDYRKKADHFAKMVIDTILRNTILADKMISYLAKTEKISEVAAIQKLEELTPLAKIVLGAKPKPVEAKEDDAEEDDENIEIPDGAKPGPIDVDYKSKMKPSAVYEAPEHTIKTWGRKGTSNGIMGVWGEFVSVDYDICIADGGCIEACPVGVYEWFDTPGNPASDKKPLMSKEPDCIFCLACEGVCPPQAIKIFEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPU4</accession>
    <name>A0A2S2KPU4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876190.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPU4"/>
    <dbReference type="GeneID" id="76209682"/>
    <dbReference type="OrthoDB" id="5664at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="124" mass="14577" checksum="802FA3636B49BFF5" modified="2018-07-18" version="1">MDDFEKEFPEFDWKNIPAYKHPGGKDCPCPKHEYIREQINFTKQVNEKGKEPSKVTLKFCPDHYRVYMNEVIPAMPLKYKILTKIALKLGAIQVEQLKYMESELCFYCKFGSGGHDRKNELPPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPV1</accession>
    <name>A0A2S2KPV1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">3-isopropylmalate dehydratase</fullName>
            <ecNumber evidence="6">4.2.1.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">leuC_1</name>
        <name evidence="17" type="ORF">NZNM25_04740</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876343.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPV1"/>
    <dbReference type="GeneID" id="76209826"/>
    <dbReference type="OrthoDB" id="6900at2157"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01583">
        <property type="entry name" value="IPMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000007">
        <property type="entry name" value="3-isopropylmalate dehydratase large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01026">
        <property type="entry name" value="LeuC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004430">
        <property type="entry name" value="3-IsopropMal_deHydase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050067">
        <property type="entry name" value="IPM_dehydratase_rel_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033941">
        <property type="entry name" value="IPMI_cat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00170">
        <property type="entry name" value="leuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004016">
        <property type="entry name" value="PRK05478.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009116">
        <property type="entry name" value="PRK12466.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822:SF9">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="15" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="14" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="16">
        <location>
            <begin position="7"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00014371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GBH33683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="471" mass="51838" checksum="A1E05B4D250B7C70" modified="2018-07-18" version="1">MGKTLFEKIWESHVIVEKPNSPSLIYIDRHLVHEVTSPQAFDGLRMNNRKVRRPDLTIATMDHNVPTNDRSLPILDQTSAVQIQTLEKNCKDFGITLFDINSPNQGIVHVIGPQLGITLPGSTIVCGDSHTSTHGAFGALALGIGTSDVEHVLASQTMWLEKPKPFEIRVEGKRKNPHAVTAKDIVLSIIKNIGTGGGTGTVIEYRGEGIEDLSMEQRMTICNMSIEAGARAGLIAPDEKTFDYLRGRKYTPRNYESLVDTWKVYLKTDADAKFEKQFTLHIDDIAPQVSWGTNPGMTCDVTESIPSPDDYSKGDPNQKKGAQKALDYMDLKPGTPIEEIKIDRVFIGSCTNARLEDLIEASRVVRGQKISPNVRAMVVPGSQMVKKQAEEMGLDKIFIDANFEWREAGCSMCLGMNPDILSPGERCASTSNRNFEGRQGTGGRTHLVSPVMAAAAAIHGHFVDVRKLDLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPV2</accession>
    <name>A0A2S2KPV2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_04870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPV2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="14"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="48" mass="5809" checksum="36C36D225D016271" modified="2018-07-18" version="1">MSDQLKQCAVFILKSQYKIRLKKFKHTNEELELAKLQSEKEKKKKELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPZ2</accession>
    <name>A0A2S2KPZ2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_05510</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPZ2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="53" mass="6415" checksum="1D7D7DE86A00C972" modified="2018-07-18" version="1">MSLFGRFKLRLPNQFFVHGDDEFVDIWSDPFEPKERLMEFRTEIQNKIAQTPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPZ9</accession>
    <name>A0A2S2KPZ9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Class I SAM-dependent methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876184.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPZ9"/>
    <dbReference type="GeneID" id="76209675"/>
    <dbReference type="OrthoDB" id="7386at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="273" mass="30838" checksum="01D75F3935610AC3" modified="2018-07-18" version="1">MVEHLAKNLVELKKEFSKMYDGGSQIQEIIPKSKSELFPIEDHDLKLLHEFATKNPIYYDSFEKTIGQTDCIVYEGDINKYWLNSIQHSSSRAPFSPTWIMSGYIGALFAKKFGYSEVIDIGSGDGRIAFCAKVLGLESYSIEIDDVLVELQKQLTTILDFRPFCADAVHFDYLSLNLTHPIFFIGGLAQMGGITLSSGVLEKIYSIPNLRKNSGWVFAGTHSKKYTPDPKNKAGWGTLIEKNNLKYVQTISLPTVWTFHEPDETPYIFAVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ09</accession>
    <name>A0A2S2KQ09_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Dolichol-phosphate mannosyltransferase</fullName>
            <ecNumber evidence="8">2.4.1.83</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Dolichol-phosphate mannose synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Dolichyl-phosphate beta-D-mannosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Mannose-P-dolichol synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">NZNM25_05470</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25 26">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O-mannosylation of proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.83" evidence="8"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ09"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004582">
        <property type="term" value="F:dolichyl-phosphate beta-D-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06442">
        <property type="entry name" value="DPM1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000119">
        <property type="entry name" value="Dolichol-phosphate mannosyltransferase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039528">
        <property type="entry name" value="DPM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007267">
        <property type="entry name" value="GtrA_DPMS_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398:SF1">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04138">
        <property type="entry name" value="GtrA_DPMS_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16" id="KW-0464">Manganese</keyword>
    <keyword evidence="15 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="11 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="23">
        <location>
            <begin position="8"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="GtrA/DPMS transmembrane" evidence="24">
        <location>
            <begin position="256"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00053724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00074878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00082336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00082614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00083744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF04138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="GBH33756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="380" mass="42596" checksum="06299476188FF475" modified="2018-07-18" version="1">MLQSTEISIIVPTYNESKNICGILEHIQKSIPKNLKAETIVVDDNSPDKTAKIAEDYFYSIKEKTDHTINVIKRKAKNGLSSAILNGIQHAAGNTIVVMDSDFSHPPHIIPKMIETLRQTRCDIVIASRYIKGGSIQGWPFKRKLMSKIATGIAKRGLGIQPHDPMSGFFMFKKNIIKGLKFDAIGYKMLLEILVKTKGAKIQEVPYTFTDRQEGASKLGASTVLDYCKSVWKLYKYGRSIKERRISVRFLSKAARFFTVGASGLGVNYLASMLFSLSADMWYIHATMMGIAFSITSNFVLNKYWTFEDKDFSAKRTITQYGKFAGFSSIGALVQLGMVYYLVDEGGISYPISLVLAVAVAAFSNFILNKKWTFKEKVWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ55</accession>
    <name>A0A2S2KQ55_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NAD(P)-dependent alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_03760</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876221.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ55"/>
    <dbReference type="GeneID" id="76209718"/>
    <dbReference type="OrthoDB" id="73567at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030554">
        <property type="term" value="F:adenyl nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043168">
        <property type="term" value="F:anion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08240">
        <property type="entry name" value="6_hydroxyhexanoate_dh_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050129">
        <property type="entry name" value="Zn_alcohol_dh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401:SF4">
        <property type="entry name" value="D-ARABINOSE 1-DEHYDROGENASE (NADP(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="6">
        <location>
            <begin position="8"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="358" mass="37677" checksum="8D06A8D54E211C49" modified="2018-07-18" version="1">MKSARITGPNEPLAVSESDTPKPQGSQVLLKVKSVGVCHSDLHLWEGGYDLGDGQFMKVTDRGVKYPVTPGHEIVGTVEDLGDDVSGISKGDSVLVFPWIGCGECPACKVGNENLCDAPRSMGVFQDGGYSDYALIPNFKYLAKLDGVDPDSATSLACSGLTAYNAVKKANANSPEFLLIIGAGGLGLMAIQIAKAITKAKIICIDSDDKKFDTAKKMGADFVVNTNVSGSLSSGTGSPVDKIISICNGKGADSVIDFVNAPQTVRTALGVIRKRGNIVLVGLFGGSLEVSLVTIPLKSIVIQGAYTGNYTDMVELLDLARKGIINPVISKRYKLDEANNALEDLKARKIVGRAVINP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ81</accession>
    <name>A0A2S2KQ81_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable cytosol aminopeptidase</fullName>
            <ecNumber evidence="6">3.4.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Leucine aminopeptidase</fullName>
            <shortName evidence="6">LAP</shortName>
            <ecNumber evidence="6">3.4.11.10</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Leucyl aminopeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">pepA</name>
        <name evidence="8" type="ORF">NZNM25_06170</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.</text>
            <dbReference type="EC" id="3.4.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.</text>
            <dbReference type="EC" id="3.4.11.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="6">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peptidase M17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.1" evidence="6"/>
    <dbReference type="EC" id="3.4.11.10" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876466.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ81"/>
    <dbReference type="GeneID" id="76209971"/>
    <dbReference type="OrthoDB" id="18419at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00433">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00181">
        <property type="entry name" value="Cytosol_peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011356">
        <property type="entry name" value="Leucine_aapep/pepB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000819">
        <property type="entry name" value="Peptidase_M17_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023042">
        <property type="entry name" value="Peptidase_M17_leu_NH2_pept"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008283">
        <property type="entry name" value="Peptidase_M17_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002073">
        <property type="entry name" value="PRK00913.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002074">
        <property type="entry name" value="PRK00913.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002083">
        <property type="entry name" value="PRK00913.3-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963:SF23">
        <property type="entry name" value="CYTOSOL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963">
        <property type="entry name" value="LEUCINE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00883">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02789">
        <property type="entry name" value="Peptidase_M17_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00481">
        <property type="entry name" value="LAMNOPPTDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00631">
        <property type="entry name" value="CYTOSOL_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 8" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytosol aminopeptidase" evidence="7">
        <location>
            <begin position="335"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="492" mass="53633" checksum="26591456F732E56D" modified="2018-07-18" version="1">MKIKTESSIKKKTDLLCGFVLENSNKVLGLSKIDRKIALSVNQSLKDMEGKLGKLSIIPLPEDKYTKRILLAGLGKREEWTEDTLRNVSGKIAQKARELKLKEFSIILPPSFEIDPISSISQIIEGSKMALYKFDKFKAEKTENEPNLTIIVSESNKISKAIRTSEIVADSAIFTKSIANLPPNECTPTTLANFAKMISKKNKMKCSVISKLELKKKGFGGISAVGQGSKNEPKLIVLEHNNGPTNQKPIVLVGKAVTFDTGGISLKPGDKMDEMKFDKCGGCTVLGIMKAVSELRLPINVVGIIPSVENMPGGESYRPGDIIKLYSGKTAEILNTDAEGRLILADALSYGEKQFSPKAIIDFATLTGACIIALGTNVAAIVSNDEKLTRKINDSSKRTSEEIWELPLNQDYMDMIKSDVADMKNVGIGRAAGTITAAAFLKNAINNTPWIHFDIAGVAWTQTATKEKSYNPKGATGFGVRLILDYLQKLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQB1</accession>
    <name>A0A2S2KQB1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein translocase SEC61 complex subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_05280</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQB1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.820">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023391">
        <property type="entry name" value="Prot_translocase_SecE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008158">
        <property type="entry name" value="Translocase_Sec61-g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00327">
        <property type="entry name" value="secE_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00584">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103456">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="44" mass="4943" checksum="2A351110DAA3F262" modified="2018-07-18" version="1">MKMAKKPDKDEYQQHLRLVLMGIAGVGAIGFTIQFVFSVITFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQC7</accession>
    <name>A0A2S2KQC7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_06570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866828.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQC7"/>
    <dbReference type="GeneID" id="76210014"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="211" mass="23880" checksum="D6DCD74B17DC2EE5" modified="2018-07-18" version="1">MKVLPNEGMSGVDSLLAESLERIIRDNLGENTSRKIQDRLFEKFGISITSAMREFDKIDYVLREFFGAGAAGLEKKFLKEICSIKSNKDKSEKRFAISDSKISQSIVKAFCDDEMSKILNASIGEPWTISEIIEKLNLPRTSGYRKINFLIEQGLLVKTGFGFTGNRRAVDKYKSLFDNVNIDFNNKVTVNVQFTPEVIRNSSILQIVYGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQD4</accession>
    <name>A0A2S2KQD4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transketolase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">NZNM25_06150</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876464.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQD4"/>
    <dbReference type="GeneID" id="76209969"/>
    <dbReference type="OrthoDB" id="25494at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016857">
        <property type="term" value="F:racemase and epimerase activity, acting on carbohydrates and derivatives"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004802">
        <property type="term" value="F:transketolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006091">
        <property type="term" value="P:generation of precursor metabolites and energy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006163">
        <property type="term" value="P:purine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00429">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02012">
        <property type="entry name" value="TPP_TK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000191">
        <property type="entry name" value="ribulose-phosphate 3-epimerase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000056">
        <property type="entry name" value="Ribul_P_3_epim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051424">
        <property type="entry name" value="Transketolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004076">
        <property type="entry name" value="PRK05581.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43195:SF1">
        <property type="entry name" value="FI06132P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43195">
        <property type="entry name" value="TRANSKETOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00834">
        <property type="entry name" value="Ribul_P_3_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00456">
        <property type="entry name" value="Transketolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Transketolase N-terminal" evidence="12">
        <location>
            <begin position="12"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH33824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="555" mass="61825" checksum="07AE1F08F6576FA5" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQD8</accession>
    <name>A0A2S2KQD8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">succinate dehydrogenase</fullName>
            <ecNumber evidence="4">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">NZNM25_06670</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="4"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876506.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQD8"/>
    <dbReference type="GeneID" id="76210026"/>
    <dbReference type="OrthoDB" id="23539at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.700.10:FF:000003">
        <property type="entry name" value="Fumarate reductase flavoprotein subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.80.40:FF:000003">
        <property type="entry name" value="Fumarate reductase flavoprotein subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.100">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.80.40">
        <property type="entry name" value="succinate dehydrogenase protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003952">
        <property type="entry name" value="FRD_SDH_FAD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014006">
        <property type="entry name" value="Succ_Dhase_FrdA_Gneg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004724">
        <property type="entry name" value="PRK06069.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01812">
        <property type="entry name" value="sdhA_frdA_Gneg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE 2 FLAVOPROTEIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632:SF51">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000171">
        <property type="entry name" value="SDHA_APRA_LASPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00504">
        <property type="entry name" value="FRD_SDH_FAD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="12">
        <location>
            <begin position="8"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal" evidence="13">
        <location>
            <begin position="446"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000171-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GBH33876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="570" mass="63509" checksum="FEFDB5538A36CE4B" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQF0</accession>
    <name>A0A2S2KQF0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AbrB/MazE/SpoVT family DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_05420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876403.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQF0"/>
    <dbReference type="GeneID" id="76209894"/>
    <dbReference type="OrthoDB" id="379381at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="67" mass="7817" checksum="29659B93CE183E95" modified="2018-07-18" version="1">MPKWKEDAKEFTVSVNYNENRGYQSSIPKPIMDLLGDPEKVTFVIDGKIIKIKSSDELSFKEKKRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQH4</accession>
    <name>A0A2S2KQH4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_06550</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQH4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="75" mass="8287" checksum="15C411B521E192A6" modified="2018-07-18" version="1">MPKMTLRRSGSTDAVAMPVTDRVFSNERIDADCAFASGYNAPKEIDTASTASIAITSCFDFFMILDNDILKIRFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQI5</accession>
    <name>A0A2S2KQI5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Succinate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_06650</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876504.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQI5"/>
    <dbReference type="GeneID" id="76210024"/>
    <dbReference type="OrthoDB" id="10105at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase, transmembrane subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000701">
        <property type="entry name" value="SuccDH_FuR_B_TM-su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01127">
        <property type="entry name" value="Sdh_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="Fumarate reductase respiratory complex transmembrane subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="12622" checksum="BA2DE75A45C5B464" modified="2018-07-18" version="1">MRESTIMKIHYGTALAAVALVAVHVLMRLTMGFAESLEYESVIANYKFIPYAAMLELILILLSIHGFNGLRVILLELKQGQLYEKAVSYGCLAAMFGLIAYGSRTIIMTNMGMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQJ6</accession>
    <name>A0A2S2KQJ6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_06750</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876513.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQJ6"/>
    <dbReference type="GeneID" id="76210035"/>
    <dbReference type="OrthoDB" id="25239at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051450">
        <property type="entry name" value="Gfo/Idh/MocA_Oxidoreductases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055170">
        <property type="entry name" value="GFO_IDH_MocA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377:SF1">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22725">
        <property type="entry name" value="GFO_IDH_MocA_C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="GFO/IDH/MocA-like oxidoreductase" evidence="2">
        <location>
            <begin position="127"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="311" mass="35091" checksum="219F1F498FFC859A" modified="2018-07-18" version="1">MKIVQIGTGGWGKNHTRILSQLGVLSAICDANPQKSKEYGDKYSVKHYDSLDELLNSEEFDGAFVVTPTSTHTEIAKKLLEAKKHVFVEKPMTYKSEDGELLAKLAEKNKVILTCGYIERFNPAVDEVKKLVKGKKFGDLVMLEFHRENRMPLHIKDVGIIYDTSVHDIDTANWLFDDMPHVVFARAGKIKHEHEDFASIMLGYKDDRVAIISSNWITPKKVRKFNAVCTDAIISSDFITQEIIVEKDNHNETIQNEKQEPLLLEIQSFLGAIEGKNEHIVKSQEAVNVTKIAEAALLSSLKGIPIYLDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQR9</accession>
    <name>A0A2S2KQR9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_07900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146195981.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQR9"/>
    <dbReference type="GeneID" id="76208886"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="105" mass="11877" checksum="05BC81BD2B2EFDE7" modified="2018-07-18" version="1">MNYKLLFLLVVLGMSILVFVWGTTNYLRCYTDTPSGNYYESCPRTPDVHAIPAVIATFVMIGIVPIITWIKTKSHKLQIVFTVLSGVGIFFVFVGILYLILMVRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQU9</accession>
    <name>A0A2S2KQU9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (guanine(37)-N(1))-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.228</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">M1G-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNA [GM37] methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">NZNM25_06190</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>guanosine(37) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36899"/>
            <dbReference type="Rhea" id="RHEA-COMP:10145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10147"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.228"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.228" evidence="1"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876468.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQU9"/>
    <dbReference type="GeneID" id="76209974"/>
    <dbReference type="OrthoDB" id="8079at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.110:FF:000001">
        <property type="entry name" value="tRNA (guanine(37)-N1)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.110">
        <property type="entry name" value="Met-10+ protein-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056744">
        <property type="entry name" value="TRM5/TYW2-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF36">
        <property type="entry name" value="TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25133">
        <property type="entry name" value="TYW2_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="10">
        <location>
            <begin position="22"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH33828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="275" mass="30869" checksum="7E4303628A041F04" modified="2018-07-18" version="1">MLKKALSSVLSEKESEDLISAFDQIGDIIIVRIPNSLLPKKKIIGETLLNEVKIARSVFYQASDVEGDFRTRNLEILAGEDNTETEYKEFGCKFIVDVEKAFFSPRLSTERERIANLIQDGEVVTNMFAGVGMFSVMAAKKKKCTVYSLDINPVASKLCEKNIELNKLTGKIISINGDASRIIEEQLENKSDRTLMLLPERSDEFLKSAISTTKNEGIIHYYSHIHADKKSEAGKLSEEHYLQVTPVKSEILGSKIVRAVGPRYYQTVVDVKISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQV0</accession>
    <name>A0A2S2KQV0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Arcadin 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQV0"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="14295" checksum="FA348516FFC3ABE2" modified="2018-07-18" version="1">MHCIFQFVKDKSSKKSNRILSATNEFKITQIVDNGQMVQLTLIEDVSTEPISQKQMIIENVSKRLDEETKEQVMPLLEAILQAQPTVNIKSYQQTQITIAMPKSRYDNMGRPQVGNTIDVNLKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQX6</accession>
    <name>A0A2S2KQX6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876703.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQX6"/>
    <dbReference type="GeneID" id="76208814"/>
    <dbReference type="OrthoDB" id="1055at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="124" mass="14665" checksum="DA44C116D56B93F4" modified="2018-07-18" version="1">MSIQGMYMLNTSQYTEEKIHESLELLYMDRKNEFRELSQVFLGKKKLSEMPNWKEFILNFCLDVENSFKTWNGKVQPSTTSPQKALTILRQVGNNMTSMNQLTHILNISYNLSAEFKEIYKRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQZ1</accession>
    <name>A0A2S2KQZ1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876729.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQZ1"/>
    <dbReference type="GeneID" id="76208778"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="132" mass="14740" checksum="B412B03122343E74" modified="2018-07-18" version="1">MEKRTKIIIIGIACLLGATWFLWVPITLMTASGWFSSFVTAITSDEEYVKKASQNPTVRQYIESYPEYTTNQHNEFLGWRVIHFDVEKGPSMYVKVSNLHGGIKVSATCPNSQGISFSQNATLAQQIVTDGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR29</accession>
    <name>A0A2S2KR29_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_09000</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876738.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR29"/>
    <dbReference type="GeneID" id="76208769"/>
    <dbReference type="OrthoDB" id="11638at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050535">
        <property type="entry name" value="DNA_Repair-Maintenance_Comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30337">
        <property type="entry name" value="COMPONENT OF ATP-DEPENDENT DSDNA EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30337:SF0">
        <property type="entry name" value="NUCLEASE SBCCD SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="418" mass="46870" checksum="97ABE203A30D880B" modified="2018-07-18" version="1">MLFAHLSDVHLGFQKHESLQKIEQHVFEKIMDECISRKVDFILIPGDLFHVNIPEMRVQKFAFKKFREVYDAGIPIYVVYGSHDFSPVSNSVIDLLAEIGYITKVTKATSNENDTISLDFLIDEKTGAKIAGLSGLKVGKDREWYEKLDRDSLEAESGFKIFLFHGGISEMKADSGMDGDHMPLSLLPKGFSYYAGGHMHKYNHQSFDGYPNVVYPGTPFAGYHADLEDNANGQRRGFALVEFDDVVKSVEFVEIPNTSYEIIEVNADNRKADSINQELVEKTKDIDPADKVVIIKVRGELTSGKTADVDISAIRDSLNERNAMVVNVSKNGLTSKEYSITEAKGNNKEEIEMNVFSENIGQLRFDHAELLGNEGIKLAKNLLQELGQPILVNEKKNEYVPRIRNNALAILGLDKDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR38</accession>
    <name>A0A2S2KR38_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Putative [LysW]-aminoadipate semialdehyde/glutamate semialdehyde transaminase</fullName>
            <ecNumber evidence="6">2.6.1.118</ecNumber>
            <ecNumber evidence="6">2.6.1.124</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">argD</name>
        <name evidence="6" type="synonym">lysJ</name>
        <name evidence="8" type="ORF">NZNM25_09410</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in both the arginine and lysine biosynthetic pathways.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[amino-group carrier protein]-C-terminal-gamma-(L-lysyl)-L-glutamate + 2-oxoglutarate = [amino-group carrier protein]-C-terminal-N-(1-carboxy-5-oxopentan-1-yl)-L-glutamine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:41952"/>
            <dbReference type="Rhea" id="RHEA-COMP:9714"/>
            <dbReference type="Rhea" id="RHEA-COMP:9715"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:78501"/>
            <dbReference type="ChEBI" id="CHEBI:78526"/>
            <dbReference type="EC" id="2.6.1.118"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[amino-group carrier protein]-C-terminal-gamma-(L-ornithyl)-L-glutamate + 2-oxoglutarate = [amino-group carrier protein]-C-terminal-gamma-(L-glutamyl-5-semialdehyde)-L-glutamate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:52672"/>
            <dbReference type="Rhea" id="RHEA-COMP:13327"/>
            <dbReference type="Rhea" id="RHEA-COMP:13328"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:136761"/>
            <dbReference type="ChEBI" id="CHEBI:136763"/>
            <dbReference type="EC" id="2.6.1.124"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
        <text evidence="6">Binds 1 pyridoxal phosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-arginine biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (Thermus route): step 4/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. LysJ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.118" evidence="6"/>
    <dbReference type="EC" id="2.6.1.124" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876775.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR38"/>
    <dbReference type="GeneID" id="76208729"/>
    <dbReference type="OrthoDB" id="85346at2157"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00038"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000004">
        <property type="entry name" value="Acetylornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02084">
        <property type="entry name" value="LysJ_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004636">
        <property type="entry name" value="AcOrn/SuccOrn_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037537">
        <property type="entry name" value="LysJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00707">
        <property type="entry name" value="argD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002325">
        <property type="entry name" value="PRK01278.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF79">
        <property type="entry name" value="ACETYLORNITHINE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 6 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="5 6 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 8" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="100"/>
            <end position="101"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="212"/>
            <end position="215"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="393" mass="42885" checksum="86F6E78029F5C85D" modified="2018-07-18" version="1">MSEDQFMGSLYQRFPVTVERGVGSHVWDSNGKEYIDCMGGYGVALVGHQNQRVNNAIKEQVDKIITVHSSLYNKTREEFLKTLIGLAPKGLTQVHLNNSGAEAIEAAIKFARKFTGKKGMVAMKGSYHGKSLGALSLTFNPKYKKAFEPLVEKVSFASFGDIESVRAAIDDDTAFVILEPIQGESGIIVAPEGFLQDVRKLCDEKGILLIFDEIQAGLGRTGRLWACEHWNTAPDILCLAKGIAGGVPMGATLVRPDILAAMSKGEHSSTFGGNPLSCAAGTAALKALTEDGLIENSEKMGKIFREGLEKLKEKHTIIREIRGKGLMIGIELKFEVRDILMGLIDKGVLMLYSGRNILRVLPPLVISEDDITKVLHALDSVLTEEEQKRDVQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR49</accession>
    <name>A0A2S2KR49_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chemotaxis protein CheA</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">NZNM25_07160</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_160049198.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR49"/>
    <dbReference type="GeneID" id="76208959"/>
    <dbReference type="OrthoDB" id="293137at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00088">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000016">
        <property type="entry name" value="Chemotaxis protein CheA, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.180">
        <property type="entry name" value="CheA-289, Domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.560">
        <property type="entry name" value="Histidine kinase CheA-like, homodimeric domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="entry name" value="HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051315">
        <property type="entry name" value="Bact_Chemotaxis_CheA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004105">
        <property type="entry name" value="CheA-like_dim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037006">
        <property type="entry name" value="CheA-like_homodim_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036061">
        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002545">
        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008207">
        <property type="entry name" value="Sig_transdc_His_kin_Hpt_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43395:SF10">
        <property type="entry name" value="CHEMOTAXIS PROTEIN CHEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43395">
        <property type="entry name" value="SENSOR HISTIDINE KINASE CHEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01584">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02895">
        <property type="entry name" value="H-kinase_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01627">
        <property type="entry name" value="Hpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00260">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01231">
        <property type="entry name" value="H-kinase_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00073">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50341">
        <property type="entry name" value="CheW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="Histidine-containing phosphotransfer domain, HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
        <property type="entry name" value="CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50894">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="HPt" evidence="14">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="12">
        <location>
            <begin position="135"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="CheW-like" evidence="13">
        <location>
            <begin position="387"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphohistidine" evidence="11">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GBH33925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="517" mass="57937" checksum="ECADC73F53A15ABC" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR54</accession>
    <name>A0A2S2KR54_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cheY</name>
        <name evidence="2" type="ORF">NZNM25_07190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876584.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR54"/>
    <dbReference type="GeneID" id="76208956"/>
    <dbReference type="OrthoDB" id="2830at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052048">
        <property type="entry name" value="ST_Response_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228:SF1">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ARR22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="1">
        <location>
            <begin position="6"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="125" mass="13628" checksum="F01CA2C738D1942D" modified="2018-07-18" version="1">MGDGVKVLIADDASFMRTVLKDILKSNGLATEIVEAPDGVEAVRQYIQHKPDLVTMDVNMPKADGIQALKGIMKVNPNARVIMVTSVEQKHIVQDAMKSGARDYIIKPFDKGNVGLVLNKALRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR63</accession>
    <name>A0A2S2KR63_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histidine kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_09610</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876792.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR63"/>
    <dbReference type="GeneID" id="76208709"/>
    <dbReference type="OrthoDB" id="8127at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00075">
        <property type="entry name" value="HATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065:SF10">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065">
        <property type="entry name" value="SENSOR HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="114"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="315" mass="35757" checksum="0A3AC60D2941180F" modified="2018-07-18" version="1">MKSKVIIGSLMVIQILAIMFTIQFLSISNQSHDTIAFVPQVFSQTPTDDFSQNEKFDFKPDYKESFVISDVNFLLVNSLSSLVMILGVVKLLKNENNKKIKTEKLAIIGELSSRIAHDMRNPLSVIQMTLENFKIQYPLSKEQERQFEKINRSIFRITHQIDDIWDFVKSPTAEKKNCSLAHLLQESLRRVNIPANIKIRLPPDDCKILCDDEKTIVVFVNIFINAIQAFEDDGNIWVSFDSNKNWITVKIEDDGVGIDPKVLDRIFEPLITTKQTGTGLGLSCCKSIMESQGGLIKASNRPKKGSAFTIHFSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR73</accession>
    <name>A0A2S2KR73_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Toprim domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_09710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR73"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39964:SF2">
        <property type="entry name" value="UPF0292 PROTEIN MJ1624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39964">
        <property type="entry name" value="UPF0292 PROTEIN TK1411"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110455">
        <property type="entry name" value="Toprim domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Toprim" evidence="1">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="101" mass="12036" checksum="9CDFAD07F0161C29" modified="2018-07-18" version="1">MVVVEGKRDTNALRKLGYSGRVLEFHKFRGMIDFADSVAKYQNLIILFDRDRKGRHLTWKTIQLLQRRTNIDLSFKRKLRLITKGKIMFIEQLVCYESFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRA1</accession>
    <name>A0A2S2KRA1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_09930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1404"/>
            <end position="1440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1404"/>
            <end position="1425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="1700" mass="182692" checksum="A340C39FBB791709" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRB9</accession>
    <name>A0A2S2KRB9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HEPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_09970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876822.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRB9"/>
    <dbReference type="GeneID" id="76208670"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="74" mass="8455" checksum="DFD7699C70B63C07" modified="2018-07-18" version="1">MQLLQQKNSQSEARKSLVERTPVKERKKLSVIHEASGTPFQTSILQQARLEAEIAVYWLLSKYAQSLRAVKNSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRC6</accession>
    <name>A0A2S2KRC6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_09900</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146195991.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRC6"/>
    <dbReference type="GeneID" id="76208677"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="295" mass="33882" checksum="ED2A0AAD09F2E684" modified="2018-07-18" version="1">MKTRLLIIIGILGTFSLINHHLAYADELQPLPSDFRDLTEHEIDIRLLYDVEDIYPIPSAPLKQLSAGVSLNNIQCKPNLHLIIKYNDTPACVKSKTAQKLTDKQSWSPSEKLWKKLENQIYSITDESFCPKRCINIESVETMQQANENTSLRINLPSYIPKNYEFFKYYPNENALNIQISSNPLTENTTWGDFYLMDHGILLTYIDYPVTSDGRTQTAYWAKTQDAQNISRQDNEPIFVKERNVTFDEDLGILFLGSPSEAQFNLNDDISIIITGYVPQEEIIKIARSFFGIDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRF1</accession>
    <name>A0A2S2KRF1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Lysine biosynthesis enzyme LysX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_09380</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RimK family. LysX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876772.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRF1"/>
    <dbReference type="GeneID" id="76208732"/>
    <dbReference type="OrthoDB" id="33241at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043774">
        <property type="term" value="F:coenzyme F420-2 alpha-glutamyl ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009085">
        <property type="term" value="P:lysine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000025">
        <property type="entry name" value="Alpha-aminoadipate--LysW ligase LysX protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000058">
        <property type="entry name" value="Alpha-aminoadipate--LysW ligase LysX protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054562">
        <property type="entry name" value="LysX/ArgX_preATP_grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011870">
        <property type="entry name" value="LysX_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004666">
        <property type="entry name" value="Rp_bS6_RimK/Lys_biosynth_LsyX"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02144">
        <property type="entry name" value="LysX_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00768">
        <property type="entry name" value="rimK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621:SF2">
        <property type="entry name" value="COENZYME GAMMA-F420-2:ALPHA-L-GLUTAMATE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 MODIFICATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22626">
        <property type="entry name" value="LysX_preATP_grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="11">
        <location>
            <begin position="95"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH34147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="285" mass="32095" checksum="5BFC1164F136D81E" modified="2018-07-18" version="1">MSKVCIVFDRLRAEEKMLQKEASDLGHDAVMLDAKITQINTDSKKDDYDFGDVVLERCVSYFRGLHFTASLEFLDVPVLNKFEVANICGNKMFMTLLLKKNNVPTPRTYFSFSSQSAAENLEKVGFPLVIKPVIGSWGRGVMPLKDKDTMEAVFEIRDITDSPHDRIYYLQELIKRPPRDIRVITVGDQPIAAMYRKSSGGFKTNIALGADPELCEISKEMEDMAVKASKAMGGGILGIDMMEDDQKGLIVHEVNNTVEFKGLARVSKRNIPKEIVEFALNYVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRG8</accession>
    <name>A0A2S2KRG8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidyl-tRNA hydrolase</fullName>
            <ecNumber evidence="2">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_08360</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = an N-acyl-L-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PTH2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876682.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRG8"/>
    <dbReference type="GeneID" id="76208837"/>
    <dbReference type="OrthoDB" id="6075at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02430">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1490.10:FF:000001">
        <property type="entry name" value="Peptidyl-tRNA hydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1490.10">
        <property type="entry name" value="Bit1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023476">
        <property type="entry name" value="Pep_tRNA_hydro_II_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002833">
        <property type="entry name" value="PTH2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00283">
        <property type="entry name" value="arch_pth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003314">
        <property type="entry name" value="PRK04322.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649:SF11">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01981">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102462">
        <property type="entry name" value="Peptidyl-tRNA hydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="117" mass="12859" checksum="45F700580E35513C" modified="2018-07-18" version="1">MGDIKQVIVVRTDLKMGKGKIAAQVGHACVLGAEHVRKSHPEWYEEWWGGQEKVVLKVSGIKELQEVKRHAIDLNLPWSEVTDAGHTQIAPGTTTCISIGPAPENLIDKITSDLKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRI0</accession>
    <name>A0A2S2KRI0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_10150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRI0"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="95" mass="10798" checksum="DA3CA9A1B3246CEF" modified="2018-07-18" version="1">MFVLIIYGAIGYLFFKYLWPMLHSNDPEKKPKELYTFYDKHSKLFVATIIFALLGINEAISMIGGIFEFIFIPCYPDSGTISISCWIKGGLGFGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRK5</accession>
    <name>A0A2S2KRK5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_09620</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876793.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRK5"/>
    <dbReference type="GeneID" id="76208708"/>
    <dbReference type="OrthoDB" id="2830at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17535">
        <property type="entry name" value="REC_NarL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050595">
        <property type="entry name" value="Bact_response_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058245">
        <property type="entry name" value="NreC/VraR/RcsB-like_REC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591:SF3">
        <property type="entry name" value="RESPONSE REGULATORY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591">
        <property type="entry name" value="STRESS RESPONSE REGULATOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="4"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="132" mass="14740" checksum="5D55473D1629D4CC" modified="2018-07-18" version="1">MNPTVILIDDYEDITEVISEYLGFHSINVLATGRNGKQGVELYKEYLPDVVVSDFLMPKYDGLYGLEQIRKINPNAKVIMMTGSIDDNLHDELRSAGASVILPKPTPLNILIETIKRIAFDNTVQSSNQISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRK9</accession>
    <name>A0A2S2KRK9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Quinolinate synthase</fullName>
            <ecNumber evidence="3 11">2.5.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_08790</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from iminoaspartate: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.72" evidence="3 11"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRK9"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00327"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008987">
        <property type="term" value="F:quinolinate synthetase A activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034628">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-aspartate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10800:FF:000007">
        <property type="entry name" value="Quinolinate synthase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10800">
        <property type="entry name" value="NadA-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003473">
        <property type="entry name" value="NadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036094">
        <property type="entry name" value="NadA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00550">
        <property type="entry name" value="nadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006878">
        <property type="entry name" value="PRK09375.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006879">
        <property type="entry name" value="PRK09375.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30573:SF0">
        <property type="entry name" value="QUINOLINATE SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30573">
        <property type="entry name" value="QUINOLINATE SYNTHETASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02445">
        <property type="entry name" value="NadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142754">
        <property type="entry name" value="NadA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="NCBIfam" id="TIGR00550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH34088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="297" mass="32905" checksum="69A91C3B27DE5114" modified="2018-07-18" version="1">MVILAHNYQIPDVQDVADFVGDSLGLSRQAANVDQQTILFCGVNFMAETAAIISPDKKVLLPDLDAGCSLSDSITVDELRNWKNQHPNAIAVGYVNTTAEIKAELDYCCTSSNAVNVVKSIPEEKEILFLPDMFLGSYVAKMTGRKNMHIWAGECHVHAGIRPEDVSEKLDSMKDAEFIIHPECSCTTPMMYDVADGSFDDKKVSILSTEGMLNHVNKSKSKNFVVATETGILYRMRQQNPGKTFVPASEKAECQYMKMITLEKVHDALVNEKNIVTVPKDIADKARLAINRMLEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRM0</accession>
    <name>A0A2S2KRM0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146195989.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRM0"/>
    <dbReference type="GeneID" id="76208773"/>
    <dbReference type="OrthoDB" id="12289at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="202" mass="23061" checksum="45C981E9251457CF" modified="2018-07-18" version="1">MKIRFLMIFAFVMIVPITESFAEPIEIKFGETVQYEDLNLSFYDIEDSRCPLDVTCIWEGKVTAMVHTSNQTHKIGAGFEIGYPLTHITPYTITLLDVKPHPISTEKPDYVAILDISKIGPESYPVPGNLCPPGKVFDWDICVDECPFGQIERNGICHSFENVYDSNDKLPWYFLIVAYWPVSIIIVGIIVTVIIVVWRKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRM3</accession>
    <name>A0A2S2KRM3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Type II toxin-antitoxin system HicB family antitoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08890</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRM3"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051404">
        <property type="entry name" value="TA_system_antitoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035069">
        <property type="entry name" value="TTHA1013/TTHA0281-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34504">
        <property type="entry name" value="ANTITOXIN HICB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34504:SF4">
        <property type="entry name" value="ANTITOXIN HICB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143100">
        <property type="entry name" value="TTHA1013/TTHA0281-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="76" mass="8528" checksum="84BCC3A4803D4FAB" modified="2018-07-18" version="1">MTNMQVSQKSRKFTILVNKEKEGGYSGKCLELPGAISQGETLKELKINMADAIKLVLEYIQDKAKKEKNKIIEITA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRS0</accession>
    <name>A0A2S2KRS0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877007.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRS0"/>
    <dbReference type="GeneID" id="76208481"/>
    <dbReference type="OrthoDB" id="9837at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050764">
        <property type="entry name" value="CbbQ/NirQ/NorQ/GpvN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42759:SF1">
        <property type="entry name" value="MAGNESIUM-CHELATASE SUBUNIT CHLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42759">
        <property type="entry name" value="MOXR FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="1">
        <location>
            <begin position="22"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="264" mass="29708" checksum="F903E3FA72B4CC54" modified="2018-07-18" version="1">MEEVQYLDWNNSIQILNKAFEAGLFVLIIGPKGTGKTSLVRDFAQSKNMNLQSINFSLRTRESHLVGSKTLTDGTVTFDEGVLIKSMREGNTLYLDEINAAEADVLLRLDEALDDRRQIVLKESTGELIKAKENWFVIGTINPLTHSGTKELPPQILSRFPVRIRLEYPPEDIELEIVKKHVSGKHESEIIQAIKLANTLRQAAAVEELFYSPSLRETIAFAKLLDKGMSSKDAAKFVFGNVYTQWGNIEYQKVSDIITSMFGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRS5</accession>
    <name>A0A2S2KRS5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thiamine biosynthesis protein ThiS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CGI121/TPRKB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876954.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRS5"/>
    <dbReference type="GeneID" id="76208553"/>
    <dbReference type="OrthoDB" id="11483at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2380.10">
        <property type="entry name" value="CGI121/TPRKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013926">
        <property type="entry name" value="CGI121/TPRKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036504">
        <property type="entry name" value="CGI121/TPRKB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011465">
        <property type="entry name" value="PRK14886.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08617">
        <property type="entry name" value="CGI-121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143870">
        <property type="entry name" value="PF0523-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="257" mass="28711" checksum="71D068298F160D43" modified="2018-07-18" version="1">MIAVKLVGGAKKSFLKEILEIDKSDISIQELLELLLELKPVDSPKLDIENILIAINGVDSSAMNGKSTIIKNNDLVSIIPVIHGGTSKKITFEILSKQIQIIEIKGKSTIGVKFIDDLRKKYPKILFQAVSSNFIMNVYHLKKILSLSLESKKNNILLSNKLETDILMRFALTTQISDAIMTAGIKPRTNFILIAIGNKKFLNLLFDDLSTLSVNLFSKKNDSFLKKHFKITQKQIDSAYSKNPLEDILVEKAAVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRT5</accession>
    <name>A0A2S2KRT5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11500</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRT5"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="346" mass="38168" checksum="54D540E5015B1F36" modified="2018-07-18" version="1">MILLLVPFSYAFSAESTDTAPTLTVSLLNKTPFVYQDSEGYAVVVGLVENNDPLSSVTNIRIQVKFFDDYNPNPLEIVQGSTTLEVISANGKSPYSIRSETPNPNITQASVSLLGFDSSVEKQKGLSVYSSDVFLDSSLRFSGVLKNTGAPNSDTKVYLAFYDGFEPSRIIAVSTIELGDVEPNTEVPFEINEKINPKSVGFLLFAESNIFNSNFVDVKIPPPQLMTKLVSISNVSVKDTKGNNLSEIKLGSTVNIESQTLVEFSEKEIPAETPYTYYVQIKESGELPYVEFVGKFDGRFIGNGPQSQSIDWIPEKKGLFFIETFVWDRNNVPISDQGPFVLILVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRY1</accession>
    <name>A0A2S2KRY1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRY1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="126" mass="14184" checksum="4432282CAB0CE706" modified="2018-07-18" version="1">MVTLLENDDYSYTISIMFSLMASILVLAIGIGITVALVGSVMFQILIPLNEDVLSPLEEKCQQIANEGYKIHSLYPDSNPEDLLESDMKRLMYLDDVWIKECVSVLPADSIFNIVNNVDRNFSYGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRY3</accession>
    <name>A0A2S2KRY3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_12070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877056.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRY3"/>
    <dbReference type="GeneID" id="76209298"/>
    <dbReference type="OrthoDB" id="3344at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="107" mass="12343" checksum="9E8A5F9819123E69" modified="2018-07-18" version="1">MNKSLGDYNIEKSSSYNIKNIVWTKKKSTNIEKITKNNFNKKFQKDSHKNIIYGINPKSKSTAKVFKIEEISDAEKLKNKKNNPSKKFQKSSASNCNIKQGFKRPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRY9</accession>
    <name>A0A2S2KRY9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ArnR1-like winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876951.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRY9"/>
    <dbReference type="GeneID" id="76208556"/>
    <dbReference type="OrthoDB" id="4292at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038723">
        <property type="entry name" value="ArnR1-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14947">
        <property type="entry name" value="HTH_45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ArnR1-like winged helix-turn-helix" evidence="1">
        <location>
            <begin position="5"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="95" mass="10830" checksum="392A57219768D4B6" modified="2018-07-18" version="1">MAEYRTHMKIIGDILSTTRDDLQDEDGATVTYLIRKANISHSRISRILKTLVSQGLLEQIDSQGSNKYRISQTGREFLQAYNTFTNFADNFGLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS14</accession>
    <name>A0A2S2KS14_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GIY-YIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_12530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877093.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS14"/>
    <dbReference type="GeneID" id="76209251"/>
    <dbReference type="OrthoDB" id="4212at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00719">
        <property type="entry name" value="GIY-YIG_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.10">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01541">
        <property type="entry name" value="GIY-YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIY-YIG" evidence="1">
        <location>
            <begin position="22"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="105" mass="12391" checksum="CE20B2F04D31379E" modified="2018-07-18" version="1">MELLEDKMRVWLESAKFVKPASGVYVLYNRNKEPIYIGETDNLEETFTKYVDTNFEGNECKQKTQSYQRVFLENPKELQTKLIEDFKNETGKMPSCNSEIQLETH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS30</accession>
    <name>A0A2S2KS30_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein eL21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl21e</name>
        <name evidence="7" type="ORF">NZNM25_10560</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876869.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS30"/>
    <dbReference type="GeneID" id="76208606"/>
    <dbReference type="OrthoDB" id="6295at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.70">
        <property type="entry name" value="Ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00369">
        <property type="entry name" value="Ribosomal_eL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001147">
        <property type="entry name" value="Ribosomal_eL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022856">
        <property type="entry name" value="Ribosomal_eL21_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036948">
        <property type="entry name" value="Ribosomal_eL21_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20981">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01157">
        <property type="entry name" value="Ribosomal_L21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="99" mass="11231" checksum="FBA5105CFA9F5C12" modified="2018-07-18" version="1">MATKKSHGRSHGFKHKSRSVMKKVSPRGVSFLLREYQEGQQALVIIDPRQHKGLPHRRYHGKVGVITNVGRRAITLDVKLGEKSKTLITRLDHIKPFGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS54</accession>
    <name>A0A2S2KS54_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Amidohydrolase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_10790</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876933.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS54"/>
    <dbReference type="GeneID" id="76208580"/>
    <dbReference type="OrthoDB" id="34429at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01292">
        <property type="entry name" value="metallo-dependent_hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032465">
        <property type="entry name" value="ACMSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240">
        <property type="entry name" value="2-AMINO-3-CARBOXYLMUCONATE-6-SEMIALDEHYDE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240:SF19">
        <property type="entry name" value="CATALYTIC_ HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="3">
        <location>
            <begin position="3"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="11"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="266" mass="30982" checksum="3E91369A8C12B785" modified="2018-07-18" version="1">MIIDCHVHVNQYELLQNVSSLDDRIEELQNEMNGNNVDYAIILSSYKVNSKRPSTKQIIEAIKKYDNLGVAAGFTIDNHTEEDLREYRELIKNGKIKALKIYSGYEHYYPYDEKYQKVYDICVEFGIPAMFHTGDTYSEKGKLRFARPLNLDEVAVDNPELKIVMCHLGNPWIQDAQEVIYKNKNVYADVSGLVVGSFDHFFEKMMKEKVAELINYAGEPRYLLYGTDWPISSMDSYLNFVAKLNIKKEFRDNFMFKNAKKLFNIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS61</accession>
    <name>A0A2S2KS61_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11720</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877008.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS61"/>
    <dbReference type="GeneID" id="76208480"/>
    <dbReference type="OrthoDB" id="8373at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00198">
        <property type="entry name" value="vWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="1">
        <location>
            <begin position="348"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="520" mass="59459" checksum="52292ABA21AA82EA" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS87</accession>
    <name>A0A2S2KS87_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">1,4-dihydroxy-2-naphthoate octaprenyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">menA</name>
        <name evidence="9" type="ORF">NZNM25_13230</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Quinol/quinone metabolism; menaquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS87"/>
    <dbReference type="UniPathway" id="UPA00079"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042371">
        <property type="term" value="P:vitamin K biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13962">
        <property type="entry name" value="PT_UbiA_UBIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026046">
        <property type="entry name" value="UBIAD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929">
        <property type="entry name" value="1,4-DIHYDROXY-2-NAPHTHOATE OCTAPRENYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929:SF0">
        <property type="entry name" value="UBIA PRENYLTRANSFERASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005355">
        <property type="entry name" value="UBIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="100"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="205"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="255"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="279" mass="29887" checksum="B19DE3DAD67C5726" modified="2018-07-18" version="1">MIAVSVGLALNGWQNSSIDLFDTVLIFAGVLSLHASVDLLNDFWDFKRGIDTKTKRTKMSGGTGVLPEGLLKPSAVYRAGIAFLIIGSLIGAYFVMTDGIIIAIILGFAILSIYFYSTKIVDSGLGEFFVAVKGSMIVIGTFFIQSGQINMESILGGIVVGSLSSLVLFIASFPDHDADKSKGRKTLVIAVGKEKASKLFWIFPIVSYSAIVAGVFADFFPLLSLICLLSVPLMIKSGLGLQKNYDSVENLVPSMSSTLLFSRITGALFVASFLIDLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSA4</accession>
    <name>A0A2S2KSA4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877149.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSA4"/>
    <dbReference type="GeneID" id="76209174"/>
    <dbReference type="OrthoDB" id="1209at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="68" mass="7717" checksum="8F86E7762DEF7B5A" modified="2018-07-18" version="1">MIIGKILDGEKRVKFSVEIHCNVCGKLVPGGLQTGESYYQTDGFKTELEEFKQNYLCGICRDVDRSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSC7</accession>
    <name>A0A2S2KSC7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphopantetheine adenylyltransferase</fullName>
            <ecNumber evidence="7">2.7.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Dephospho-CoA pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Pantetheine-phosphate adenylyltransferase</fullName>
            <shortName evidence="7">PPAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">coaD</name>
        <name evidence="9" type="ORF">NZNM25_11490</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(R)-4'-phosphopantetheine + ATP + H(+) = 3'-dephospho-CoA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19801"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="2.7.7.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the eukaryotic CoaD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.3" evidence="7"/>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876990.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSC7"/>
    <dbReference type="GeneID" id="76208507"/>
    <dbReference type="OrthoDB" id="53228at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004595">
        <property type="term" value="F:pantetheine-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00647">
        <property type="entry name" value="PPAT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050385">
        <property type="entry name" value="Archaeal_FAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023540">
        <property type="entry name" value="PPAT_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001985">
        <property type="entry name" value="PRK00777.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43793">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43793:SF1">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 7 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="8">
        <location>
            <begin position="9"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="158" mass="17696" checksum="D154897B480EB079" modified="2018-07-18" version="1">MSKYSLVAMGGTFDIIHKGHLTLLSNAFDISDKVIIGLTSDELAEKKKKTLINKYEKRLENLTTLLLKKYSRSSFQISKLNNDFGPAVLEKEVEALVVSDETSNQGNVLNKLREDMKLPPVQIITVPMFLAKDGKRISTTRIKNSEIDVEGNLLSIDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSG4</accession>
    <name>A0A2S2KSG4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11890</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829077.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSG4"/>
    <dbReference type="GeneID" id="76209317"/>
    <dbReference type="OrthoDB" id="6423at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="150" mass="17148" checksum="09FC40AF62D13D3C" modified="2018-07-18" version="1">MLSEEKTDVESSEIRTEALLETVSDAEVNSRISFEEFTDERILISHDAFGNKQMKIKVLEVSDETPPSKWKFGDRVKVTKILVTIKHLTTQQVEEGEFDIEAIERELAEKRHYSSTNRWIPASDIKNGYVIGSKHTRLISDASALDYIAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSM1</accession>
    <name>A0A2S2KSM1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12460</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877322.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSM1"/>
    <dbReference type="GeneID" id="76209258"/>
    <dbReference type="OrthoDB" id="84364at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="69" mass="7913" checksum="DB4E4EC509D63A5E" modified="2018-07-18" version="1">MEQKALECPVCKSNKATIAGENIKLSGRFEDKEFLTTELTVLRCTKCGYYMFFDKMAQFTTKDESDKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSR9</accession>
    <name>A0A2S2KSR9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_14840</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877282.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSR9"/>
    <dbReference type="GeneID" id="76209011"/>
    <dbReference type="OrthoDB" id="9219at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="99" mass="11237" checksum="6402BE51CFD7C5D9" modified="2018-07-18" version="1">MTEIDTQKDVYLFLHGRMDLKEKAMNALTTKGFSSDKVVMALPNKVGNVGDYMAMLWMPPNPDHIKIQEITKIEEVKPEGMIGLWKGVSKEDIDTIQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSS6</accession>
    <name>A0A2S2KSS6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Sporulation kinase C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">kinC_2</name>
        <name evidence="11" type="ORF">NZNM25_15130</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877307.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSS6"/>
    <dbReference type="GeneID" id="76208983"/>
    <dbReference type="OrthoDB" id="8127at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065:SF10">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065">
        <property type="entry name" value="SENSOR HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="PAS" evidence="9">
        <location>
            <begin position="16"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="10">
        <location>
            <begin position="88"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="174"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="128"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="383" mass="43523" checksum="CD8B16EA2B68F7E0" modified="2018-07-18" version="1">MAEKMKISKMEKQNSLEDNLRRAIDSSSIVTVMNTNGIVTHVNQKFCEISGFAENEIIGTNLLMLRSGVHPIEYYDELWATISKGEIWTGEICNQRKNGELFWNKATIIPIKNQDGEIIEYIAIRFDITNQKEKENKMEETLMKLQKSNQIIEEQSKKLVKQEKLVTIGELAARLSHDIRNPLSIIRVSLENLKIIYGKNELETKHFDRVERAIDRISHQIDDVLGFIRKEPMRFEKTLISEIIADSLDSINLPDRIYFEISKNDFEIYCDKKRLSVAITNLILNAIQGIEGKGTIEIILEDKGENIVIKIKDSGKGIPSDIIEEIFEPLFTTKQQGTGLGLASVKSIINGHGGKIFVTSPPTVFTIIIPKKGIDNNLDDPHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KST9</accession>
    <name>A0A2S2KST9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Roadblock/LAMTOR2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_14080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877220.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KST9"/>
    <dbReference type="GeneID" id="76209091"/>
    <dbReference type="OrthoDB" id="9657at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046600">
        <property type="entry name" value="DUF6659"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20364">
        <property type="entry name" value="DUF6659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="127" mass="14212" checksum="9759616B269FA9FF" modified="2018-07-18" version="1">MLKPDVALKLYGEKCDELLREEEIRFAGIVDKDGQLIAGGFKEGLVPHEGDETRLQSFLEFVSKASIRKEYDESLGPINYLAARRDKAVLVSFPFPITQILLLISAEPTTNIETLAKKVVEIFTDVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSU9</accession>
    <name>A0A2S2KSU9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Histidinol-phosphate aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">hisC</name>
        <name evidence="10" type="ORF">NZNM25_15510</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877384.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSU9"/>
    <dbReference type="GeneID" id="76208437"/>
    <dbReference type="OrthoDB" id="9929at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004400">
        <property type="term" value="F:histidinol-phosphate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005861">
        <property type="entry name" value="HisP_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01141">
        <property type="entry name" value="hisC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42885:SF2">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42885">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 10" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="9">
        <location>
            <begin position="28"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="356" mass="39962" checksum="637158EF5EAF2A94" modified="2018-07-18" version="1">MKKNWFDEKIKKFSLIGGYKKPELLKDAVKLDSNENYVISKQFQNDILLSAKKSSDVREYPLGGAERLIDMISKFIKVSSSMIGVGNGSDQILDLILSNFASKETKVLTSNPTFGFFEERCKLYSIPLIAIPFSNSMKLDVKDFMKQSKDASILYLDSPNNPTGFQFSKNDLTKLVKSFEGLVIIDEAYGEFGDYSLANMVKTQENLIVVQTLSKSFGLAGLRLGYFIANKKFTDTFMTVLQYPYPLSTITIESGISALEKSDLMKDAVNNIKTERKRVIETLQKYDSFEVFDSKANFVLFDAHGSYKRVFAALAEQGISIRKLGKIGSHEGCLRVTIGTKEMNSKFLLAIRDLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSW3</accession>
    <name>A0A2S2KSW3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Type IV pilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_13260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877150.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSW3"/>
    <dbReference type="GeneID" id="76209172"/>
    <dbReference type="OrthoDB" id="12081at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013373">
        <property type="entry name" value="Flagellin/pilin_N_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02537">
        <property type="entry name" value="arch_flag_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="20025" checksum="0C139223A9E20460" modified="2018-07-18" version="1">MTKLQSKQNKLTSRRAVAPVIATLLLVAIAVVGGSIVFVFSQGFFSSAQISGSPNIESLKFTGYDATDGTTLLNHDGTTFAAGNTAGNGLIIGESVSVYIQSNSVGKVTLGEVRFGGAVYNYTTSSPGSGDYQVLTTGPGTFLATSSAELQPGQQATIIMNLGENIKSGRDTQFKLTTANGAVFVGTIIAGQQSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSZ3</accession>
    <name>A0A2S2KSZ3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ArsR family transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13660</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877179.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSZ3"/>
    <dbReference type="GeneID" id="76209134"/>
    <dbReference type="OrthoDB" id="10985at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="12830" checksum="DD85A6AE9325CC0C" modified="2018-07-18" version="1">MQIINQLKIEEPERKEVFLEILSDKYCRLILDSIMNISKSAIEISREKKIPLSTVYRRIQQLHDSHMIRTSGIITDEGKRLFLYKSKIKEVNTRFSDGEIAMDVVFNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT42</accession>
    <name>A0A2S2KT42_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SHOCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_14190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877226.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT42"/>
    <dbReference type="GeneID" id="76209076"/>
    <dbReference type="OrthoDB" id="11556at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="18450" checksum="FB8933F157F342F3" modified="2018-07-18" version="1">MSKDNSNKDRENIFEILDGIMFQLSRTKKMFLIMIITTLIIPPLSIIAITSVYDSPYQDKFREDFDTRLQSKLDNDEITEDEYNLMKEKFSKKTKLNPLLRPPQLVIFVISVVWLGIGIRQWFVISKWDKRYQQFKEKQKEIDKKFEDDSDDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT59</accession>
    <name>A0A2S2KT59_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Histidinol dehydrogenase</fullName>
            <shortName evidence="6">HDH</shortName>
            <ecNumber evidence="6">1.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">NZNM25_15520</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14 6">
            <text>L-histidinol + 2 NAD(+) + H2O = L-histidine + 2 NADH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:20641"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 10">Belongs to the histidinol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.23" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877385.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT59"/>
    <dbReference type="GeneID" id="76208436"/>
    <dbReference type="OrthoDB" id="36308at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00014"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004399">
        <property type="term" value="F:histidinol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06572">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1980:FF:000001">
        <property type="entry name" value="Histidinol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001692">
        <property type="entry name" value="Histidinol_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022695">
        <property type="entry name" value="Histidinol_DH_monofunct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012131">
        <property type="entry name" value="Hstdl_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00069">
        <property type="entry name" value="hisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256:SF2">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS TRIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256">
        <property type="entry name" value="HISTIDINOL DEHYDROGENASE HDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00815">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000099">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00083">
        <property type="entry name" value="HOLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00611">
        <property type="entry name" value="HISOL_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="321"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="322"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="414"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="414"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="423" mass="45499" checksum="1FC8553DA1DF02AA" modified="2018-07-18" version="1">MRIIKVSNVEKFASKIIPKHPQKNKSIIESILKDVQKNGDKAIRKYEKKFSKAKITSLRLSQVEIKNAYSKVSKSELDAIRLAKTRLEKTEKSIKSLLKNKTINNDGIKITKKFIPIQSVGCYVPGGLARYPSSAIMSIVPAKVVGVPRIAVVSPPNSDGTIDPLTIVAADICGANEIYKTGGVQAIAALSFGTNTISKVDKIVGPGGAFVTGAKFLASAQTGIDMLAGPTELGIIADNSANPKFVALDLISQAEHSSDTFCYLITTSEKLAKSVDNMISELLLKIQRVNIVKSSLKNNGFIAICKTKSDMTKLANMLAPEHLQIMAKNPSSISSQITSSGLVLLGNDTPSSASDYILGSNHILPTNGFGKIRGSLSVLDFIKTNTEVICSKSSLPKLSKHLEVLTNAEGLFNHYESVRGRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT77</accession>
    <name>A0A2S2KT77_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Thermosome subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_15720</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877401.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT77"/>
    <dbReference type="GeneID" id="76208415"/>
    <dbReference type="OrthoDB" id="9362at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03343">
        <property type="entry name" value="cpn60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012714">
        <property type="entry name" value="Thermosome_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02339">
        <property type="entry name" value="thermosome_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="536" mass="57848" checksum="BCB8D37B667498DE" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT89</accession>
    <name>A0A2S2KT89_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">V-ATPase proteolipid subunit C-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_16550</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT89"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033177">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, proton-transporting domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18120">
        <property type="entry name" value="ATP-synt_Vo_Ao_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.20.10">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038662">
        <property type="entry name" value="ATP_synth_F0_csu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="6">
        <location>
            <begin position="39"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="101" mass="9946" checksum="87B7868F5372F5E7" modified="2018-07-18" version="1">MKTIVMLLLAASAISILGSTGVAFAQEDGASNGDSMKLLGAGLAFGIAAGGAGIGLGQVGAAGLAVISENPALQSKVFIFVGMVESIAIYGIVMMFIILGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT92</accession>
    <name>A0A2S2KT92_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_14690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866901.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT92"/>
    <dbReference type="GeneID" id="76209026"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="94" mass="11504" checksum="3AD8F2099D2CD4CC" modified="2018-07-18" version="1">MDEIEKIIDEISFRKAKCKDYETMKIEDISKELQDIMRFEQESLKKIEEFENMQKNQDLANYLRMISKNTTQREITEIQEIYLKKIDKEYLNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTC1</accession>
    <name>A0A2S2KTC1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH asnC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_16840</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877507.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTC1"/>
    <dbReference type="GeneID" id="76208302"/>
    <dbReference type="OrthoDB" id="6995at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF8">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR PTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13404">
        <property type="entry name" value="HTH_AsnC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="300" mass="34505" checksum="A70F7927C6163638" modified="2018-07-18" version="1">MDNLDMKILSRLLNNCRESDRQIGMELGISGGAVRARIRKMEEMKIIEEFFIKVEPPVLGYGVLYFVVSGEDINEILDQIVLVGEPFFVVPCVGGITVCSIVVKDNMEQKIQLASKLMKDVRVLSIFEAENPGFNSNLTKTDLEILDKLIKNPIAKIETIAEKTNMSTKTITRCIEKLQENDGVQFTVIYDPKKIERYIPHAILTWIDGDLKETIENMNRIFSKSYLQIPFIAKNQIVLFMYSDNIFKMDEITQKVRTVKNVKSADLFIPKKISFHIKWLEKAIKDFKKSSKLHLTYQTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTE7</accession>
    <name>A0A2S2KTE7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transcription termination factor FttA</fullName>
            <ecNumber evidence="12">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">fttA</name>
        <name evidence="15" type="ORF">NZNM25_16970</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Terminates transcription on the whole genome. Termination is linked to FttA-mediated RNA cleavage and does not require NTP hydrolysis. Cleaves endonucleolytically at the RNA exit channel of RNA polymerase (RNAP); the 5'-3' exonuclease activity of this protein degrades the nascent RNA released from RNAP.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 2 Zn(2+) ions, which are required for nuclease activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer. Interacts with RNA polymerase (RNAP), interacts with the Spt4-Spt5 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. FttA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="12"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTE7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004532">
        <property type="term" value="F:RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22532">
        <property type="entry name" value="KH-II_CPSF_arch_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16295">
        <property type="entry name" value="TTHA0252-CPSF-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00870">
        <property type="entry name" value="FttA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019975">
        <property type="entry name" value="aCPSF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050698">
        <property type="entry name" value="MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033769">
        <property type="entry name" value="TffA_KH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03675">
        <property type="entry name" value="arCOG00543"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203:SF51">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17214">
        <property type="entry name" value="KH_TffA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 12" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 12" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 12" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="13">
        <location>
            <begin position="184"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-Casp" evidence="14">
        <location>
            <begin position="413"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Metallo-beta-lactamase N-terminus" evidence="12">
        <location>
            <begin position="171"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Metallo-beta-lactamase C-terminus" evidence="12">
        <location>
            <begin position="571"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="596"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GBH34906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="629" mass="70425" checksum="843F47B5622E1AE2" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTE9</accession>
    <name>A0A2S2KTE9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidyl-prolyl cis-trans isomerase</fullName>
            <ecNumber evidence="9">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_17000</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the FKBP-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="9"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877519.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTE9"/>
    <dbReference type="GeneID" id="76208286"/>
    <dbReference type="OrthoDB" id="8615at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040825">
        <property type="entry name" value="FKBP26_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054016">
        <property type="entry name" value="FKBP26_IF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048261">
        <property type="entry name" value="SlpA/SlyD-like_ins_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47861">
        <property type="entry name" value="FKBP-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE SLYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47861:SF3">
        <property type="entry name" value="FKBP-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE SLYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18046">
        <property type="entry name" value="FKBP26_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22199">
        <property type="entry name" value="FKBP26_IF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="11">
        <location>
            <begin position="6"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH34909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="252" mass="28564" checksum="53A599172BE270E1" modified="2018-07-18" version="1">MTFDKGSLILVDYTAKVKDTEEIFDTTIEEDAKKHSIYEENVKYQPKLVSIGEVSYPVLKGLDEALAKTSAGDKLTVEVTPDKGFGERDSGKVRMIPLRKLGEDAEKVSVGDTIEVDNKRGIIRFIGSGRVQIDYNHRYAGKTILFDVNVIKSLDSPNDKIDGILKNRLPVEDTKISFILKDKEVNITIPEEILRADGLQIMKHFIQLDIFKFVPTLEKVNFIETHINKQTQEKKVEKTPEKIEKTPEQKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTG9</accession>
    <name>A0A2S2KTG9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Zinc ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_16480</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877474.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTG9"/>
    <dbReference type="GeneID" id="76208337"/>
    <dbReference type="OrthoDB" id="10909at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03235">
        <property type="entry name" value="ABC_Metallic_Cations"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000134">
        <property type="entry name" value="Iron-enterobactin ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050153">
        <property type="entry name" value="Metal_Ion_Import_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42734">
        <property type="entry name" value="METAL TRANSPORT SYSTEM ATP-BINDING PROTEIN TM_0124-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42734:SF17">
        <property type="entry name" value="METAL TRANSPORT SYSTEM ATP-BINDING PROTEIN TM_0124-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="5"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="240" mass="27660" checksum="B75FB5BBA6A5BB2D" modified="2018-07-18" version="1">MLKIVEIENLTVQYPDVKALDDVSFEVNQGDFLGIIGPNGAGKSTLFDVMLGLNTKYKGTIKFFGEDIKKSKEYLKEIGYVPQKPIFEKNFPVTVIDVVRMGLRNETDENKIDEVLQQLWIHELRNRRIGDLSEGQQQRVFIAKALITNPKILILDEPVTGIDQQNIDLFYSILRELNSKRKITIIWSSHDLDAVNQLANHVACLNRTLFFHGESEKFFSNDELIKQYSEASMQEHMHHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTI5</accession>
    <name>A0A2S2KTI5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_16820</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_264953722.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTI5"/>
    <dbReference type="GeneID" id="76208304"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42798:SF7">
        <property type="entry name" value="ALPHA-D-RIBOSE 1-METHYLPHOSPHONATE 5-TRIPHOSPHATE SYNTHASE SUBUNIT PHNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42798">
        <property type="entry name" value="LIPOPROTEIN-RELEASING SYSTEM ATP-BINDING PROTEIN LOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="1"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="195" mass="21369" checksum="158158BF49143D79" modified="2018-07-18" version="1">MGSSGSGKSTLLNMIGLLDRPTNGKIFIDKIDTTTLDDNNISSFRNKKLGFIFQFSNLLSDLTVLENILLPRQIAGTNHTSEKDARDLLKAVGLENQMNKRANKISGGQAQRVAIARGLINKPSIVLADEPTGNLDSVTSETIVQLMKSMAKKLNQTFIIVTHDRQHFGDVDKVITIKDGRAFEGEQPSQMEVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTM8</accession>
    <name>A0A2S2KTM8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_17220</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTM8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="74" mass="8422" checksum="DEB65E8CF6189BD0" modified="2018-07-18" version="1">MSILFMTLEDGLNLIENYKKALQKFLDTLPEQSVQLGSEMIQTLTMNSKNEMKNLDAIEKALKKPPQYESGLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTR6</accession>
    <name>A0A2S2KTR6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Precorrin-2 C(20)-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cbiL</name>
        <name evidence="9" type="ORF">NZNM25_18350</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877634.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTR6"/>
    <dbReference type="GeneID" id="76210286"/>
    <dbReference type="OrthoDB" id="23546at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030788">
        <property type="term" value="F:precorrin-2 C20-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11645">
        <property type="entry name" value="Precorrin_2_C20_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012382">
        <property type="entry name" value="CobI/CbiL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006364">
        <property type="entry name" value="CobI/CbiL/CobIJ_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01467">
        <property type="entry name" value="cobI_cbiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43467">
        <property type="entry name" value="COBALT-PRECORRIN-2 C(20)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43467:SF2">
        <property type="entry name" value="COBALT-PRECORRIN-2 C(20)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036427">
        <property type="entry name" value="Precrrn-2_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="4 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="8">
        <location>
            <begin position="4"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR036427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH35044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="240" mass="26470" checksum="C7AD938DE0BC81AF" modified="2018-07-18" version="1">MPGLIGIGVGPGDPELLTVKAVNAINNADIIMCPASKEDRPSIALSVVSSLIDKSKNQQIVKLIFPMTKDKDILEQTWKKNAKIMAETVLSGKNVVYLTVGDPYLYSTWIYMHKDLKENYPDMEISVIPGIVSMFTFASKVGVSIAEGAEKVAIIPSCYDLSSVKEIAKNSETMVFLKDGRYFDQVIQVLKESGFPDDSIFAIGQDLGTDHEIIRKLTLGEVNDDTLTTKYFSILVVKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTU1</accession>
    <name>A0A2S2KTU1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_18370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877636.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTU1"/>
    <dbReference type="GeneID" id="76210284"/>
    <dbReference type="OrthoDB" id="2921at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="222" mass="22550" checksum="1FB529191688A632" modified="2018-07-18" version="1">MKNATGIGMLAAITFAVGMVGLNFSDGTFDLQSNTASSYSEGSSILGHIEVIHTDSEGNILSYQQTDNAIVNDGRNCTAMLLFGANSGCTAATAASLGKYTVIGISNGSALADTTANTVLPAEITVGDGLNAVAGTLGTFTNSTGDSDPAVQRISNQFTYDSGASQTGNQINAAGLFNQTATGADRGVFALKNFPSTVTMNPGDQLTVNWDITIDGSDAVGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTU6</accession>
    <name>A0A2S2KTU6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative snRNP Sm-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_18540</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877678.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTU6"/>
    <dbReference type="GeneID" id="76210080"/>
    <dbReference type="OrthoDB" id="371816at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01731">
        <property type="entry name" value="archaeal_Sm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044641">
        <property type="entry name" value="Lsm7/SmG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022901">
        <property type="entry name" value="snRNP_Sm-like_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553:SF5">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="4">
        <location>
            <begin position="5"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH35063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="78" mass="8458" checksum="21CF7C36DCDD7303" modified="2018-07-18" version="1">MSVDMAVKVLDESINQVVLIKLKGNKTIRGMLLGFDQHMNLLLDSSEEIPTEGDSKSLGSIVVRGDNVVMISPPPAQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTX5</accession>
    <name>A0A2S2KTX5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_18840</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877707.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTX5"/>
    <dbReference type="GeneID" id="76210111"/>
    <dbReference type="OrthoDB" id="6546at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="83" mass="9769" checksum="47E1BFEE897556F7" modified="2018-07-18" version="1">MTEFSSEEKTILVQYVIKKFENEEIVLEKLKNILPEKDIQRNIDTLIGTQRVRRIGPENLQNNESHTELPELPEHLKSIIEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTX6</accession>
    <name>A0A2S2KTX6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Blue (type 1) copper domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_19110</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146196011.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTX6"/>
    <dbReference type="GeneID" id="76210140"/>
    <dbReference type="OrthoDB" id="4392at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000923">
        <property type="entry name" value="BlueCu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028871">
        <property type="entry name" value="BlueCu_1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052721">
        <property type="entry name" value="ET_Amicyanin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507:SF1">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00127">
        <property type="entry name" value="Copper-bind"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00196">
        <property type="entry name" value="COPPER_BLUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Blue (type 1) copper" evidence="6">
        <location>
            <begin position="148"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Blue (type 1) copper" evidence="6">
        <location>
            <begin position="246"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH35120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="322" mass="34475" checksum="19E0E5D97F0E53E4" modified="2018-07-18" version="1">MKLSHDNSQVYRTTPARTGKMMIIMLGICIVGGAIFFSMWDYWISQPAPVVGMMSETIEHDVQAAFTGVHIPVELSFIESPDFRTLAFNALPGDPNHNPTITMNVGDEINFSVVNHGKSFHSFGVTAADEGFGGIIPGSEVAAASNPLKPGEGGESTFIAGEEGTYYYICTVPGHREQGMVGKIIVGDVPVEEVMETAPEPEVMEEVAEPEVMEEVAEPEPVAYDGVISIPDGSSIPGCDETNECYIPYHVTVSAGDEITWSNDDSAAHTVTSGTPSGGPDGNFDSSLFMAGNTFSVTLDEAGEYPYFCMVHPWMVGIVTVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTZ9</accession>
    <name>A0A2S2KTZ9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cysteine desulfurase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_17360</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877546.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTZ9"/>
    <dbReference type="GeneID" id="76208249"/>
    <dbReference type="OrthoDB" id="5817at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586:SF8">
        <property type="entry name" value="CYSTEINE DESULFURASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="2">
        <location>
            <begin position="18"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="383" mass="43338" checksum="5FE7AF2F811B3831" modified="2018-07-18" version="1">MNLTSKDISDDFPDSNKIYLNNASVSLMPSQSIDDMKEFLISYNSIGPDSKDSEPFVAEKLQNVRKIIAKIISCQPDEVVLTQSTTDGINFVANGISFDDKSNIILRGMGHEHHANYYPWLQLKDKISIKNLPIDKDGFFKLHDLEYFIDDNTKLVILSHALYNTGSILPVEKIGKILDNKIPFFLDSAQTIGCIGEIDVSKIRCNFMSFNGSKWLCGPMGTGLFYCSRKSSELLKPMTIGGESAIIYDETNLAFKELPDKFQTGFRNYVGIVGLESSVRYLLNFGMKNIREKNLYLSNLFREELSKIPNIILHGPDDPNHRTSIVSFNLKGQDSQEIVDKLEKQNIVLAVREIMNQKIIRASPHFFNTESEMLQTIDAIKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU27</accession>
    <name>A0A2S2KU27_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gins51 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_17660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877572.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU27"/>
    <dbReference type="GeneID" id="76208219"/>
    <dbReference type="OrthoDB" id="82417at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11714">
        <property type="entry name" value="GINS_A_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21695">
        <property type="entry name" value="GINS_B_archaea_Gins51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054314">
        <property type="entry name" value="Gins51_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22090">
        <property type="entry name" value="Gins51_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gins51 C-terminal" evidence="1">
        <location>
            <begin position="134"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="185" mass="21346" checksum="FAC600598ADFA415" modified="2018-07-18" version="1">MSESNQIDVNSLHHTVLREIENDSLLEIDPNFYRDLADFIGNLRKQEFDGVESKIKDTMIEMVTELSSLLINIRLDKILHSSEIEIGYLLDEEKFILDSKEEQKERVEMIISATIHGKSKFLESLAQNHKTKKIVIRFLNEVDEIVGADLEKYGPFKPEDIATIPYENAQALIAKNIATKVRWED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU71</accession>
    <name>A0A2S2KU71_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ATPase AAA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_19940</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. CDC48 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877800.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU71"/>
    <dbReference type="GeneID" id="76210225"/>
    <dbReference type="OrthoDB" id="77269at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19511">
        <property type="entry name" value="RecA-like_CDC48_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000007">
        <property type="entry name" value="AAA family ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000057">
        <property type="entry name" value="AAA family ATPase, CDC48 subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000018">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005938">
        <property type="entry name" value="AAA_ATPase_CDC48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01243">
        <property type="entry name" value="CDC48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="7">
        <location>
            <begin position="7"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48" evidence="6">
        <location>
            <begin position="104"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="210"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="483"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH35203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="728" mass="80092" checksum="C3520D4BF4DF10F6" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU93</accession>
    <name>A0A2S2KU93_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cobalt-precorrin-4 C(11)-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cbiF</name>
        <name evidence="7" type="ORF">NZNM25_18360</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877635.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU93"/>
    <dbReference type="GeneID" id="76210285"/>
    <dbReference type="OrthoDB" id="6633at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046026">
        <property type="term" value="F:precorrin-4 C11-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11641">
        <property type="entry name" value="Precorrin-4_C11-MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006362">
        <property type="entry name" value="Cbl_synth_CobM/CibF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050161">
        <property type="entry name" value="Siro_Cobalamin_biosynth"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01465">
        <property type="entry name" value="cobM_cbiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790:SF4">
        <property type="entry name" value="COBALT-PRECORRIN-4 C(11)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790">
        <property type="entry name" value="SIROHEME SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="6">
        <location>
            <begin position="4"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH35045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="252" mass="27848" checksum="713ED9C9B6D64E14" modified="2018-07-18" version="1">MSDVFFVGCGPGDPELITIKAKKLIQKADVVVYSGSLIPEPILNLCKKGNLFDAAKLVREEIFDLLYKNAKKGKLVVRLHDGDPSIYGAIKEQIDNLEKNGINSVVVPGVTAFLASAAALGTQLTLPGVTQTIIVTRAESRTKVPKRESISELAKHKATLIFYLSVHLLSKLVKEAIEGGYKKTTPVAVVYRASWEDQKIVKGTLEDIAKKVKDEKITRTAIVIISDVIDPESYEYSKLYDKNFSHGYRKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU94</accession>
    <name>A0A2S2KU94_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UDP-N-acetyl glucosamine 2-epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">neuC</name>
        <name evidence="2" type="ORF">NZNM25_19770</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877786.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU94"/>
    <dbReference type="GeneID" id="76210207"/>
    <dbReference type="OrthoDB" id="7018at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006047">
        <property type="term" value="P:UDP-N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020004">
        <property type="entry name" value="UDP-GlcNAc_Epase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003331">
        <property type="entry name" value="UDP_GlcNAc_Epimerase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029767">
        <property type="entry name" value="WecB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03568">
        <property type="entry name" value="NeuC_NnaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43174">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE 2-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43174:SF3">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE 2-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02350">
        <property type="entry name" value="Epimerase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDP-N-acetylglucosamine 2-epimerase" evidence="1">
        <location>
            <begin position="24"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="380" mass="42985" checksum="4F7FEC9C54FCD882" modified="2018-07-18" version="1">MRKKILFVTERRADYSKLRPVINEIKKSKKFDYYLVVTGSHLLKKHGYTINEIKNDGFHIYKKFKMFEENDDDSPATMTMAFGKAVINLTKIIKSLKPDLIFSGFDIGANFAAAIIGAHMNIHVAHLEGGEITGTIDESIRHAISKFAHIHFTSNTQATKRLIKMGELPKNIFTVGNPSLDVIKSIKFIPKKKLEDEFNINLEKPLLLVVQHTVTTEINKIDKYFSETINAIKDTNIQTIIISGNVDAGSQKIKKIIKNSNISNYENLPFIKYISLLYYSSAIIGNSSSGIMEAPFLHIPSINIGTRQEGRGKTKSIINVKYDKNKIKMAINKVLTDKKFLNSIKKQKNQHGNGIASKKIIQILEKLNFENISIQKKLAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUC6</accession>
    <name>A0A2S2KUC6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA primase large subunit PriL</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">priL</name>
        <name evidence="9" type="ORF">NZNM25_20300</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Regulatory subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Stabilizes and modulates the activity of the small subunit, increasing the rate of DNA synthesis, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="7">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimer of a small subunit (PriS) and a large subunit (PriL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the eukaryotic-type primase large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877849.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUC6"/>
    <dbReference type="GeneID" id="76210265"/>
    <dbReference type="OrthoDB" id="46081at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06560">
        <property type="entry name" value="PriL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00701">
        <property type="entry name" value="DNA_primase_lrg_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058560">
        <property type="entry name" value="DNA_primase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007238">
        <property type="entry name" value="DNA_primase_lsu_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023642">
        <property type="entry name" value="DNA_primase_lsu_PriL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10537">
        <property type="entry name" value="DNA PRIMASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10537:SF3">
        <property type="entry name" value="DNA PRIMASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04104">
        <property type="entry name" value="DNA_primase_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140914">
        <property type="entry name" value="PriB N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="6 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 7" id="KW-0639">Primosome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA primase large subunit C-terminal" evidence="8">
        <location>
            <begin position="232"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH35239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="341" mass="38522" checksum="BF2C48A15CA4693C" modified="2018-07-18" version="1">MIALGQDEIAKYPFLADAGQYLKDQGFSLEQFGTDPDLKQLIDKAYDRILVAADGKIYKSDITGDHVSKEAALPREVFSFLLAIVLLKLCGMHTLIKRFALAEARRAEKYLERDLSNISDESKKELAIKVIDDLFSVQIEKQDDYFVIPISDYLKHSINFHEREWKLINRHVEKGLVFLTPHETVRLIRKELGTYINSKIINAKTPTMIPGFEDSVNKLALLSKKFATFTVTTGEYPPCIKHAIEILEKGENLPHSGRFMLATFLLSKGQSVQQIAPLFKNAPDYNPRVTLYQLNHLAGTSGSGTQYSCPSCEKLKTQSLCFATSECDNIINPLQFGKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUD0</accession>
    <name>A0A2S2KUD0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cob(I)alamin adenosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_20170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877827.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUD0"/>
    <dbReference type="GeneID" id="76210251"/>
    <dbReference type="OrthoDB" id="24392at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008817">
        <property type="term" value="F:corrinoid adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00561">
        <property type="entry name" value="CobA_ACA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003724">
        <property type="entry name" value="CblAdoTrfase_CobA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00708">
        <property type="entry name" value="cobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004637">
        <property type="entry name" value="PRK05986.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46638">
        <property type="entry name" value="CORRINOID ADENOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46638:SF1">
        <property type="entry name" value="CORRINOID ADENOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02572">
        <property type="entry name" value="CobA_CobO_BtuR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015617">
        <property type="entry name" value="Adensltrnsf_CobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="176" mass="19584" checksum="72B350F70A568AFE" modified="2018-07-18" version="1">MKDGLIIVYTGKGKGKTTAALGIALRAVGYEKRICMIQFIKGSWHYGEMDSSKRLEPEFEMVAVGKGFVGIIDDKSSKEEHKEIANEAIKISNEKIQSGKYDIVILDEINYAVNLGLIPVNDVLNLIKSKPQEVDLVLTGNYAKDEVINLADLVTEMREIKHPFQKGIKAKKGIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUQ5</accession>
    <name>A0A2S2KUQ5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glucose-1-phosphate thymidylyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_19690</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUQ5"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04189">
        <property type="entry name" value="G1P_TT_long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005908">
        <property type="entry name" value="G1P_thy_trans_l"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01208">
        <property type="entry name" value="rmlA_long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42883">
        <property type="entry name" value="GLUCOSE-1-PHOSPHATE THYMIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42883:SF2">
        <property type="entry name" value="THYMIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="1">
        <location>
            <begin position="26"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="2">
        <location>
            <begin position="294"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="375" mass="41567" checksum="E79AC90887DE66C3" modified="2018-07-18" version="1">MLKKINIKSIIFRLLKLEEKSSSRMKGIILHGGHGTRLRPLTHTGPKQLLPIANKPMSNYCVETLIQAGIHDIVFIVGGVGSEKVNEFYGNGEKFGAKFSYIEQDFPKGIAHAISLCQEFIDGEKFVVFLGDNIINKDISKFISDFKTSNSFASLLLCEVENPSQFGIAEIKNNKIINITEKPKNPSSNLAVTGIYCLTPHIFEVIKKLTPSWRNELEIADALQILIKEDKEVTFEMITDFWKDTGTPKDIIEANRKILENMEGFQKGEKEEGVKITGKVMIDEGVKIENGVKITGPVIIGKNCIIGENSEIKGNTSIGENSEIKGCVISDSIIMSNCVLEGKFEIKNSIIGSNSRIQKNQDKKFLLGEGSQISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUR9</accession>
    <name>A0A2S2KUR9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cobyrinate a,c-diamide synthase</fullName>
            <ecNumber evidence="7">6.3.5.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Cobyrinic acid a,c-diamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cobB</name>
        <name evidence="7" type="synonym">cbiA</name>
        <name evidence="10" type="ORF">NZNM25_20160</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the ATP-dependent amidation of the two carboxylate groups at positions a and c of cobyrinate, using either L-glutamine or ammonia as the nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>cob(II)yrinate + 2 L-glutamine + 2 ATP + 2 H2O = cob(II)yrinate a,c diamide + 2 L-glutamate + 2 ADP + 2 phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26289"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58537"/>
            <dbReference type="ChEBI" id="CHEBI:58894"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 10/10.</text>
    </comment>
    <comment type="domain">
        <text evidence="7">Comprises of two domains. The C-terminal domain contains the binding site for glutamine and catalyzes the hydrolysis of this substrate to glutamate and ammonia. The N-terminal domain is anticipated to bind ATP and cobyrinate and catalyzes the ultimate synthesis of the diamide product. The ammonia produced via the glutaminase domain is probably translocated to the adjacent domain via a molecular tunnel, where it reacts with an activated intermediate.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="7">The a and c carboxylates of cobyrinate are activated for nucleophilic attack via formation of a phosphorylated intermediate by ATP. CbiA catalyzes first the amidation of the c-carboxylate, and then that of the a-carboxylate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the CobB/CbiA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.11" evidence="7"/>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877825.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUR9"/>
    <dbReference type="GeneID" id="76210250"/>
    <dbReference type="OrthoDB" id="8896at2157"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00231"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042242">
        <property type="term" value="F:cobyrinic acid a,c-diamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05388">
        <property type="entry name" value="CobB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03130">
        <property type="entry name" value="GATase1_CobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00027">
        <property type="entry name" value="CobB_CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004484">
        <property type="entry name" value="CbiA/CobB_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00379">
        <property type="entry name" value="cobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002204">
        <property type="entry name" value="PRK01077.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43873">
        <property type="entry name" value="COBYRINATE A,C-DIAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43873:SF1">
        <property type="entry name" value="COBYRINATE A,C-DIAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51274">
        <property type="entry name" value="GATASE_COBBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="8">
        <location>
            <begin position="6"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="CobB/CobQ-like glutamine amidotransferase" evidence="9">
        <location>
            <begin position="245"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <feature type="site" description="Increases nucleophilicity of active site Cys" evidence="7">
        <location>
            <position position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH35225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="451" mass="50338" checksum="D48964B36F900E3F" modified="2018-07-18" version="1">MKIPRIVIAGATSGVGKTSITCSIIHSLQKEGYSVQPFKVGPDYIDPSYLSSVSKKETYNLDVWLMGKNQLLDSFVLNSNSSVSVIEGVMGYYDGFGGDTNYASTHHVASITKSPVILILDASKTSRSIAATALGFIKFHRNSGIVGIILNKIGSKKHELLCKSALEKTKIPIIGVIPKNPELNLESRHLGLISTLDDKSLKTKIQRISKIISQYVDVQQIIKISKSATPLEKKPKPIAKKTKTTIAVALDTSFNFYYQDNLKALQREGANLKFFSPVEDREIPKCDGLYIGGGFPEILGSSLEKNQIMKKTIKKLSEDNLPIYAECGGLMYLTKSILSNNKKHKMVGIFDAETKMTKKMRLNYTKGRICKKNLISEKLHNFQGHEFHYSQLDSISNDSKFAYTLEIGEGIQKHQDGMIEYNTLASYGHLYFDSSNYAQIFVKNCLEYSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNP6</accession>
    <name>A0A2S2KNP6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00610</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875939.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNP6"/>
    <dbReference type="GeneID" id="76209390"/>
    <dbReference type="OrthoDB" id="978at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="86" mass="10048" checksum="B932CA69C7979383" modified="2018-07-18" version="1">MGKTIFIKEIILILKEPRLCPTCEKEDKLEKDVVREERTNGKTILCSRCEALIVITNQHPRKVELSSTRDDIIMLKEPHLIRKVSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNQ9</accession>
    <name>A0A2S2KNQ9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_00470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829016.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNQ9"/>
    <dbReference type="GeneID" id="76209376"/>
    <dbReference type="OrthoDB" id="10951at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="128" mass="14056" checksum="794980C80405955A" modified="2018-07-18" version="1">MKQYTVIIAVGIGGIGIMMLIFGMDILKLSVSTQDYDIFVDPILDKQSMFVMGRVTIQNTGYLPLTNVHVNFGAGDTLELGTINVGEKIIVSPPSDNPMEFVMISADNDIFVNKVYREMPKMVGMMGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNU4</accession>
    <name>A0A2S2KNU4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rpl22</name>
        <name evidence="10" type="ORF">NZNM25_00250</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="7 9">This protein binds specifically to 23S rRNA. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7 9">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875907.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNU4"/>
    <dbReference type="GeneID" id="76209351"/>
    <dbReference type="OrthoDB" id="314984at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.10:FF:000015">
        <property type="entry name" value="50S ribosomal protein L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="entry name" value="Ribosomal protein L22/L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01331_A">
        <property type="entry name" value="Ribosomal_uL22_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_uL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057265">
        <property type="entry name" value="Ribosomal_uL22_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005721">
        <property type="entry name" value="Ribosomal_uL22_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_uL22_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003260">
        <property type="entry name" value="PRK04223.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01038">
        <property type="entry name" value="uL22_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593:SF10">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="Ribosomal protein L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7 9" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH33234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="152" mass="17407" checksum="E60F27936AA510DB" modified="2018-07-18" version="1">MGRFNYAFQNYDATKHVRSSLREKDISHKHAREVAVSIKGLSIEKARDYLIAVVNKKRAVAFRRYKNQVGHKSDPGMMSGRYPQKTAKEFIKVLDNLESNAEYKGMDLDRLRIINATVHKGVIVKRFIPRAMGRATPKNNVLTHVELVAQEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNV1</accession>
    <name>A0A2S2KNV1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">50S ribosomal protein L6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_00350</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875918.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNV1"/>
    <dbReference type="GeneID" id="76209362"/>
    <dbReference type="OrthoDB" id="7144at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000008">
        <property type="entry name" value="50S ribosomal protein L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="entry name" value="Ribosomal protein L6, alpha-beta domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_uL6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_uL6-like_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_uL6_a/b-dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004037">
        <property type="entry name" value="PRK05518.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF16">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="60S/50S RIBOSOMAL PROTEIN L6/L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="Ribosomal protein L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="6">
        <location>
            <begin position="99"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="185" mass="21013" checksum="A49125E1F99905CD" modified="2018-07-18" version="1">MSTKQMEKFQDEVIIPEGVKITLNKHMLSFVGPLGKTFKNFRTIPVNLEITESKVILNTIGSRNKDYAILHTARSIIRNICEGLVEGYTIKMKIVYAHFPITLKVEGKKILIENFQGERAPRITKIVGNTKVIPKGEDVILTGEVWTDITQTAANIEQKTKVKNKDHRVFLDGIYAFEKKKGLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNY6</accession>
    <name>A0A2S2KNY6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nitroreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_01330</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nitroreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876004.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNY6"/>
    <dbReference type="GeneID" id="76209462"/>
    <dbReference type="OrthoDB" id="287850at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02062">
        <property type="entry name" value="Nitro_FMN_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="entry name" value="NADH Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43673">
        <property type="entry name" value="NAD(P)H NITROREDUCTASE YDGI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43673:SF2">
        <property type="entry name" value="NITROREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="FMN-dependent nitroreductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="6">
        <location>
            <begin position="54"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Nitroreductase" evidence="6">
        <location>
            <begin position="116"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="215" mass="24093" checksum="2AFD2AAD8161F69F" modified="2018-07-18" version="1">MDSGKKDERIFPLGYDPQDIPVSDNPNIRNQLLDFILQSGPTEVVDTDLFAVMAKRRSTRKFSDKPVETTKIDKIIAAADTAPTAGNYQGFEIFYIKSPEKKKLLVEACNKQPYVDAPVVLIFCKNPSRVKFDFPEYVLAKFAIQDATLAAGYSQLASQALGLSSIWIGMFDEKKVMDVIGTDLIPSSVLCIGYPEQTKFPKPRRNLKDLVHVVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP04</accession>
    <name>A0A2S2KP04_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_01600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876029.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP04"/>
    <dbReference type="GeneID" id="76209490"/>
    <dbReference type="OrthoDB" id="385567at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="122" mass="14344" checksum="6C1983CBA5B068A5" modified="2018-07-18" version="1">MTDYRAIFVAIAMLVVFFVFVVPLLLESVSNMNNQEMVENLEDKLIEYCGLSNKVSKIYEVLESLNLDTLDERTKQIYWDLGNGNEVSKCDMLYFYQQLNEDNKKKLNWFEFACNVNDCLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP14</accession>
    <name>A0A2S2KP14_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01150</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875989.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP14"/>
    <dbReference type="GeneID" id="76209444"/>
    <dbReference type="OrthoDB" id="34756at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="219" mass="25169" checksum="550F560F5EA9C955" modified="2018-07-18" version="1">MDLVKFRNDVNQMTEKLSCGLKEKDVFKLNNVCQQLIEMYQKNLVKINHSILELICASNLISRGYTVKVEKDISDILVCDIYAKKGDGDTIIEIETGFTPPEHAMDTIDYFAARIMSKIARYSQYCSKFSLATPAVGNLPISKVFLLPPNARNENDVRKIQDLCDRYYKNPPIKYDDVLNARLHSIYLINIDKGFAKEVDPQGYVELTENLLRRSEIEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP27</accession>
    <name>A0A2S2KP27_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Universal stress protein UspA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_02210</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876088.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP27"/>
    <dbReference type="GeneID" id="76209555"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF25">
        <property type="entry name" value="USPA DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="5"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="143" mass="15303" checksum="BBD7B97B9BD4D749" modified="2018-07-18" version="1">MAVKTKKILVPLDGSKNSIRGLDMAIHIARQSHGIITALSVKSVPGIYAIHPLGFLDFNSMSEIKKTLDEAKVRAAKKGIQLTGKAIAGDPGFDIAKFANNKKNGIDLVVIGARGRSSAKEIFLGSVSNYVLHKSKKPVLIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP51</accession>
    <name>A0A2S2KP51_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01550</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146195959.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP51"/>
    <dbReference type="GeneID" id="76209485"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="79" mass="9249" checksum="E26F5988B6378C86" modified="2018-07-18" version="1">MMYSRPEILKPTKVNSENLRFPCIICDDENGNRKHKVFDNIDTLDRHTKALHSKHPCYKAVKNIIRNLRKAVDLGMVRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP54</accession>
    <name>A0A2S2KP54_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2299 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00390</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875922.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP54"/>
    <dbReference type="GeneID" id="76209367"/>
    <dbReference type="OrthoDB" id="4100at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018747">
        <property type="entry name" value="DUF2299"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10061">
        <property type="entry name" value="DUF2299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="16935" checksum="6E72362C83398605" modified="2018-07-18" version="1">MSASRLRDNVERWLIHEGLSFEEIKSAENSFQILAKHAGQADIPVEIFEPKGQPGILVIGAKVVMKNNQIARYLGFNAQEKENFEKKVTDFCHSIQAINKIITEDGKQKIGVYVVLDDKENINQQTVFDAIDSVSEKHEKTSRFLLKTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP72</accession>
    <name>A0A2S2KP72_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01120</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875986.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP72"/>
    <dbReference type="GeneID" id="76209441"/>
    <dbReference type="OrthoDB" id="2479at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="204" mass="23461" checksum="F540BB4A92099355" modified="2018-07-18" version="1">MRKLGTIDLEILDLAMKANGTFNENNLEKSELKRLGIGKILDSLASLKDRKFLSLNSDGSFSITNLAKEILWSNNTPTWAKILRLLQIKSCSIKQIVDILAIREDEILENLEKLRKSQFVLMSPQRQEDKIIKIYEIMYEGIEEIDKTEEHGFENTVFSGPTHEIEIISIIDEISKNIQDSKLEQSEKQSILEKLSKLKNKLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPA8</accession>
    <name>A0A2S2KPA8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Lysine--tRNA ligase</fullName>
            <ecNumber evidence="10">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Lysyl-tRNA synthetase</fullName>
            <shortName evidence="10">LysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 12" type="primary">lysS</name>
        <name evidence="12" type="ORF">NZNM25_03140</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>tRNA(Lys) + L-lysine + ATP = L-lysyl-tRNA(Lys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="10"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876167.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPA8"/>
    <dbReference type="GeneID" id="76209651"/>
    <dbReference type="OrthoDB" id="6838at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00177">
        <property type="entry name" value="Lys_tRNA_synth_class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002904">
        <property type="entry name" value="Lys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00467">
        <property type="entry name" value="lysS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37940">
        <property type="entry name" value="LYSINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37940:SF1">
        <property type="entry name" value="LYSINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01921">
        <property type="entry name" value="tRNA-synt_1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="An anticodon-binding domain of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class I anticodon-binding" evidence="11">
        <location>
            <begin position="437"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="10">
        <location>
            <begin position="42"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="10">
        <location>
            <begin position="297"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF19269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH33523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="528" mass="59995" checksum="66850338CDCF2ACD" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPE6</accession>
    <name>A0A2S2KPE6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_01290</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPE6"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="111" mass="12230" checksum="BBA08578915ED8F0" modified="2018-07-18" version="1">MSDMRFIIIGIILVFAGFLVLGIFGHNFQAATLESDEFGTCYEYFDDKPPVKINCSFKIFEQTIFFGVIIGLIGAGIISLIKGVKGDWDNNVKPEDMVGPGGRQTDDSDKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPI0</accession>
    <name>A0A2S2KPI0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein eS31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rps27ae</name>
        <name evidence="8" type="ORF">NZNM25_03640</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eukaryotic ribosomal protein eS31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876210.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPI0"/>
    <dbReference type="GeneID" id="76209705"/>
    <dbReference type="OrthoDB" id="25142at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00777">
        <property type="entry name" value="Ribosomal_eS31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002906">
        <property type="entry name" value="Ribosomal_eS31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022845">
        <property type="entry name" value="Ribosomal_eS31_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001669">
        <property type="entry name" value="PRK00432.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01599">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01402">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Small ribosomal subunit protein eS31" evidence="7">
        <location>
            <begin position="15"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="61" mass="6924" checksum="7C576643DAB4DD24" modified="2018-07-18" version="1">MPVEQKGKKGSSPNVYKYFKVDGDKVTRVRKICSRCGKGVFMSEHKNRHTCGKCGLTEFNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPI1</accession>
    <name>A0A2S2KPI1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_01860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876054.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPI1"/>
    <dbReference type="GeneID" id="76209517"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="272" mass="30942" checksum="241054C28C0744DE" modified="2018-07-18" version="1">MVGKNTTVITTVVGLFGVSIIAFLLLSTPSDQKSIPVEDDIRIVADTKYNHYTNGETINIWGKIMNSKGMPLSEYNHAITLQILDDGDMLKEIAQVTPSSDGMFEHMISTDSSIWENGKYQLKAFYDQNLATAYFEIDGHRPNYTYEYDSDPLGNYKTWCNETGGIYSTGDRIGCQFETKEKAYNARDLLHQMEERTIEGSLAQKLCRAYGKICDQFTRITMEYDMITGELYKLKALEGRETPYTEFRIIEGDVVQYRPAVKPAVNWITFDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPI8</accession>
    <name>A0A2S2KPI8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_03150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876168.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPI8"/>
    <dbReference type="GeneID" id="76209652"/>
    <dbReference type="OrthoDB" id="11865at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="65" mass="7232" checksum="5D4E8D4B86508DD4" modified="2018-07-18" version="1">MVHGEHNKTRNNGGFAMIIFGALLLFLAPNIYPEMPELGIVSLFGGMGIGGIGFYLKFFRNRTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPJ9</accession>
    <name>A0A2S2KPJ9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Delta-aminolevulinic acid dehydratase</fullName>
            <ecNumber evidence="3 13">4.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">hemB</name>
        <name evidence="15" type="ORF">NZNM25_03910</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 13">
            <text>2 5-aminolevulinate = porphobilinogen + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="4.2.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the ALAD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.24" evidence="3 13"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876236.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPJ9"/>
    <dbReference type="GeneID" id="76209733"/>
    <dbReference type="OrthoDB" id="8493at2157"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00318"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004655">
        <property type="term" value="F:porphobilinogen synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00384">
        <property type="entry name" value="ALAD_PBGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000019">
        <property type="entry name" value="Delta-aminolevulinic acid dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001731">
        <property type="entry name" value="ALAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030656">
        <property type="entry name" value="ALAD_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006762">
        <property type="entry name" value="PRK09283.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458:SF0">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00490">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001415">
        <property type="entry name" value="Porphbilin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00144">
        <property type="entry name" value="DALDHYDRTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01004">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00169">
        <property type="entry name" value="D_ALA_DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="6 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GBH33600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="326" mass="36024" checksum="87B990BFF5680363" modified="2018-07-18" version="1">MSFPARRLRRLRTSEKMRELIQQTTLSPKDFICPVFVQDELKTRVKVESMSEIERLPLKDVNDEVGTISDLGIPAIMLFGIPAQKDEAGTSAFDDNGIVQNAISQIRKNFGNKIVIMADVCLCQFTSTGHCGIIQGDKIDNDTSLDTLAKIAVSQAKAGVDTVSPSAMMDGQVAAIRRALDDEGFTDVSIMSHSAKHRSNFYSPFRDAAECAPKFGDRKTYQVPYTNAREAMMEVETDINEGVDIVMIKPALSYLDLIAETRKKFNIPVSAYSVSGEYALVKAASQLGYVNEKDITEEILYSIKRAGADMIVTYFAKSAARFLQES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPQ4</accession>
    <name>A0A2S2KPQ4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PRC-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_04240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876301.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPQ4"/>
    <dbReference type="GeneID" id="76209772"/>
    <dbReference type="OrthoDB" id="587at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.240">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC-barrel" evidence="1">
        <location>
            <begin position="15"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="150" mass="16945" checksum="90F19FE9CE9E17C0" modified="2018-07-18" version="1">MSVKLEGMPTNIATADSFTGKKVIDREGIRYGKVKHIHIHPDTLTVSGVTIHQGFNKDYFLSEDFIDKFSEETLLLSRPPVRTGVSVVDIDGHKIGKVKRLHRNPETSELESIEVTDGLMHSKILSKSEIWGIGEKIILRMSKEEFKKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPR7</accession>
    <name>A0A2S2KPR7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">tRNA-intron lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_04470</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876549.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPR7"/>
    <dbReference type="GeneID" id="76209798"/>
    <dbReference type="OrthoDB" id="46045at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22363">
        <property type="entry name" value="tRNA-intron_lyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006677">
        <property type="entry name" value="tRNA_intron_Endonuc_cat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006678">
        <property type="entry name" value="tRNA_intron_Endonuc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036740">
        <property type="entry name" value="tRNA_intron_Endonuc_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006676">
        <property type="entry name" value="tRNA_splic"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00324">
        <property type="entry name" value="endA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21227">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21227:SF0">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01974">
        <property type="entry name" value="tRNA_int_endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02778">
        <property type="entry name" value="tRNA_int_endo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55267">
        <property type="entry name" value="tRNA-intron endonuclease N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA intron endonuclease N-terminal" evidence="2">
        <location>
            <begin position="8"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA intron endonuclease catalytic" evidence="1">
        <location>
            <begin position="82"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="187" mass="22021" checksum="036997D9DA179F63" modified="2018-07-18" version="1">MDDTPIVNGEMISDQACISDKSLIHELELKGYGEIENEKLFLKQFETLYLLYTKRLILKKIKKQIDFDTFMNICQKTDSDILTKFLIYRDLRNRGYVVKDGFGFGSDFRVYERGHFGEKGAKYLVFGLNEGQQEKMGNLQKKIEDITHMGKEPIIAVIDRRGEVIYYKINRMNFYENKARLEESFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPT6</accession>
    <name>A0A2S2KPT6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">rRNA metabolism protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_04670</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SDO1/SBDS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876337.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPT6"/>
    <dbReference type="GeneID" id="76209819"/>
    <dbReference type="OrthoDB" id="84504at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1250.10">
        <property type="entry name" value="Ribosome maturation protein SBDS, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.900">
        <property type="entry name" value="SBDS protein C-terminal domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036786">
        <property type="entry name" value="Ribosome_mat_SBDS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002140">
        <property type="entry name" value="Sdo1/SBDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039100">
        <property type="entry name" value="Sdo1/SBDS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046928">
        <property type="entry name" value="SDO1/SBDS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018978">
        <property type="entry name" value="SDO1/SBDS_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037188">
        <property type="entry name" value="Sdo1/SBDS_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019783">
        <property type="entry name" value="SDO1/SBDS_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00291">
        <property type="entry name" value="RNA_SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927">
        <property type="entry name" value="RIBOSOME MATURATION PROTEIN SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927:SF4">
        <property type="entry name" value="RIBOSOME MATURATION PROTEIN SDO1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20268">
        <property type="entry name" value="SBDS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09377">
        <property type="entry name" value="SBDS_domain_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01172">
        <property type="entry name" value="SBDS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89895">
        <property type="entry name" value="FYSH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109728">
        <property type="entry name" value="Hypothetical protein AF0491, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS N-terminal" evidence="2">
        <location>
            <begin position="5"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS central" evidence="3">
        <location>
            <begin position="98"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS C-terminal" evidence="4">
        <location>
            <begin position="162"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="228" mass="25660" checksum="E707FB9D2129EA1C" modified="2018-07-18" version="1">MADYTLVRYSYEGEKFEIMVKPDPALDYRLGKKKDISSILVSDEIYTDASKGTRPTAEKLLHAFKTEDPAEIAEIILKKGDLNLTTEQRRTMIEEKRKQIVTFIAKTYVDPKTHLPHPPLRIEQAMKDGRVSIDPHKTIDEQVKDIVEKLRSIIALKSENLKLEITIPAQFASQSYAVLKSVGSLNKEEWQNNGSLKAILEIPAAARPNVIDRLGSITKGSATVEVMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPT8</accession>
    <name>A0A2S2KPT8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04500</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876321.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPT8"/>
    <dbReference type="GeneID" id="76209802"/>
    <dbReference type="OrthoDB" id="6375at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="143" mass="15987" checksum="B9A30D1CC809C380" modified="2018-07-18" version="1">MSDELKKLMERAHDGITTFDFDGKETPCIIIEEKKYDDMLSKIAGQSLSINTDLNILQDGLGNVFVEVVLTFSKGNIVETFLINAKTHLDFFESLASTAMFALSSPKSNYGKDNVFMIQLPRPEKAQDALEIIKNALVPKNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPU2</accession>
    <name>A0A2S2KPU2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase</fullName>
            <ecNumber evidence="2">2.5.1.147</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">NZNM25_02860</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>5-amino-6-(D-ribitylamino)uracil + L-tyrosine + S-adenosyl-L-methionine = 5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil + 2-iminoacetate + 5'-deoxyadenosine + L-methionine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55200"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:77846"/>
            <dbReference type="ChEBI" id="CHEBI:85936"/>
            <dbReference type="EC" id="2.5.1.147"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="10">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme F0 biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.147" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876141.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPU2"/>
    <dbReference type="GeneID" id="76209623"/>
    <dbReference type="OrthoDB" id="8186at2157"/>
    <dbReference type="UniPathway" id="UPA00072"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141093">
        <property type="term" value="F:5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044689">
        <property type="term" value="F:7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01612">
        <property type="entry name" value="FO_synth_sub2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045567">
        <property type="entry name" value="CofH/MnqC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019940">
        <property type="entry name" value="CofH_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034405">
        <property type="entry name" value="F420"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020050">
        <property type="entry name" value="FO_synthase_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00423">
        <property type="entry name" value="CofH family radical SAM protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03551">
        <property type="entry name" value="F420_cofH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005609">
        <property type="entry name" value="PRK07360.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076">
        <property type="entry name" value="FO SYNTHASE (COFH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076:SF1">
        <property type="entry name" value="LIPOYL SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19288">
        <property type="entry name" value="CofH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004762">
        <property type="entry name" value="CHP00423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01388">
        <property type="entry name" value="7_8-didemethyl-8-hydroxy-5-dea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00343">
        <property type="entry name" value="aminofutalosine_synthase_(mqnE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00342">
        <property type="entry name" value="cyclic_dehypoxanthine_futalosi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01064">
        <property type="entry name" value="F420__menaquinone_cofactor_bio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01389">
        <property type="entry name" value="menaquinone_synthsis_involved"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Radical SAM core" evidence="12">
        <location>
            <begin position="64"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>(3R)-3-methyl-D-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:64642"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>(3R)-3-methyl-D-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:64642"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR004762-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR004762-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH33495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="419" mass="47593" checksum="9D5F50C9226246C4" modified="2018-07-18" version="1">MTINIDALLKNADPLISEILNNALSDKEISAQDGLELYKSTGVDFHLVGLVADEIRRRRVGNIVSYVVNRNINFTNVCIKQCGFCAFSRDFREEEGYFLPTEEIVRRAMEARQLGATEVCIQAGLPPDMKGDLYESICREIKKEIPDIHIHGFSPEEILYGASRSEISIEEFLKRMKEAGVDTLPGTSAEILDQKLRDQISPGRISVENWEKVIKYAHKIGINTTSTMMFGHLETLEQRVAHIVKLRDIQKETGGFTEFVPLNFIHTEAPMYKHQLHEGIRRGGSGNDVLLTHAVARIMLNNSINNIQMSWVKEGQKMSQLLLMWGANDFGGTLINESISTSAGSEHGQLLKPKEIRRRIKEIGRIPAERNTNYKILKKFDAEEEVDEKLDRILDTSQFGSYVELIKINKFKYKNPRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPW9</accession>
    <name>A0A2S2KPW9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_02990</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146195968.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPW9"/>
    <dbReference type="GeneID" id="76209636"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="309" mass="35082" checksum="D8AD31EAB78A2724" modified="2018-07-18" version="1">MELEKNFQQEKINQKEVIKLPVSVIDEAANKEKMITHAVNVIGRSQDRLKVFESIYFHKSPVKTQDHIQNETGLKSLKRVLEVGNSLVSGGVIEQTEKNKRIAYGKIPFYKKYKTIIIQRVHKKQFNVPSELSSGNGNITVTVNLKNNKHIPKELTVDDIDSFSKVKKIGSIKTKRIPEEDIKQLLKAITKEDGKFVDWGGEINDINTTRLKINGKRINTSFALKGAGTPPTLTIKKMGKHGDQISRLFKSPSQAFFVMYDAQIDHSILDMMKSLAENKSNKENKMIYYGVIDGQDTARLFRAYSKSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPX0</accession>
    <name>A0A2S2KPX0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04940</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876360.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPX0"/>
    <dbReference type="GeneID" id="76209846"/>
    <dbReference type="OrthoDB" id="378560at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="117" mass="13577" checksum="A3D8673AF552DC8E" modified="2018-07-18" version="1">MKIILFDDHMRINLNILEMLGSAKTSFYIPYTNIVSAFSEPPHQIVCFKLAGTNFPKLIELGTFLYGTQKQFCYIKRRKNNFLVLVLKSNFYSKIILEITNSEEIAKRINEKLGIPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ05</accession>
    <name>A0A2S2KQ05_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">3-isopropylmalate dehydratase small subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_04750</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LeuD family. LeuD type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876344.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ05"/>
    <dbReference type="GeneID" id="76209827"/>
    <dbReference type="OrthoDB" id="6505at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01577">
        <property type="entry name" value="IPMI_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033940">
        <property type="entry name" value="IPMI_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050075">
        <property type="entry name" value="LeuD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011827">
        <property type="entry name" value="LeuD_type2/HacB/DmdB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02087">
        <property type="entry name" value="LEUD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43345:SF2">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43345">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT 2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="3">
        <location>
            <begin position="35"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="163" mass="17922" checksum="B67443355FB2C0D9" modified="2018-07-18" version="1">MKGNIIKYAQDNIDTDVIIPGQYLKVHDYAELATHAMEGLDPDFHSKVKEGDFILSGKNFGCGSSREHAPIALSHSGIKAVLALSFARIFYRNAVDGAFLLPIEIEQDAYDGIAEGDEVEINLSANEIKNITKNQTYTMKPFSEIIGKIIAAGGLFKYKPDQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ30</accession>
    <name>A0A2S2KQ30_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_05500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ30"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="49" mass="5513" checksum="272697D884BD518D" modified="2018-07-18" version="1">MGCGKSFEGKGEKFCDNVCRDSHIAKLEERMREAVKNDPSHTKELSKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ53</accession>
    <name>A0A2S2KQ53_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_04420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146195974.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ53"/>
    <dbReference type="GeneID" id="76209791"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.570">
        <property type="entry name" value="Particulate methane monooxygenase, b subunit. Chain: A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023301">
        <property type="entry name" value="NH3_CH4_mOase_suB_N"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="217" mass="24726" checksum="F1647E121BE0323D" modified="2018-07-18" version="1">MKTRLLIITILSITCVTSTVMEVYGHGVGPRFNSGFYRVEDDMYSKTTVSVGESFILNGTIVSLVERDFTGNMNIRFDYTNNDPWLVNLLKSNFSCLAKDTCAESIHVFPNQNHWYMDIQPTPDSYLLKGDEIIFYSIEITPLKGGTYHVHTDPDADIPSFRYIGPGETIVVQGSQDITDGELFEFYIPYTIGFVLITIGLIYGIVFIYRKIQRKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ86</accession>
    <name>A0A2S2KQ86_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ86"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="142" mass="16502" checksum="A815427CAB151ACB" modified="2018-07-18" version="1">MLVMISSDFKQSLAISRLCDDILESNERIFFVSSINYNGRVFESRSRNDSVLTKLSKSETEMFFMQRTLQTSLSKEFDDIVGPLNFITVQRETFLELIFPYTEGLILVVCDLDVVPNYLAKRILFVLRDFDWASKNSQIYNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQC2</accession>
    <name>A0A2S2KQC2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MIP18 family-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_06630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQC2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052339">
        <property type="entry name" value="Fe-S_Maturation_MIP18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42831">
        <property type="entry name" value="FE-S PROTEIN MATURATION AUXILIARY FACTOR YITW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42831:SF1">
        <property type="entry name" value="FE-S PROTEIN MATURATION AUXILIARY FACTOR YITW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIP18 family-like" evidence="1">
        <location>
            <begin position="22"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="118" mass="13294" checksum="B8F7812C508AC02E" modified="2018-07-18" version="1">MVIYSIDAFAHMSQDIKQLRVKIFDELSKIVDPEINTSIVELELIDEVDINNDNVKVDLHLTSPFCPAVFGFKICQDIHDNLLKVDGVDDVKVNVSNHFMAEQINNQVNNSPNPKKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQD1</accession>
    <name>A0A2S2KQD1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peptidase S8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_05220</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQD1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07474">
        <property type="entry name" value="Peptidases_S8_subtilisin_Vpr-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051048">
        <property type="entry name" value="Peptidase_S8/S53_subtilisin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034213">
        <property type="entry name" value="S8_Vpr-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43399:SF4">
        <property type="entry name" value="CELL WALL-ASSOCIATED PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43399">
        <property type="entry name" value="SUBTILISIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="636"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="10">
        <location>
            <begin position="34"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="11">
        <location>
            <begin position="227"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH33731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="656" mass="71497" checksum="0E3019851A0D5DB4" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQM2</accession>
    <name>A0A2S2KQM2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Glutamyl-tRNA reductase</fullName>
            <shortName evidence="8">GluTR</shortName>
            <ecNumber evidence="3 8">1.2.1.70</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hemA</name>
        <name evidence="11" type="ORF">NZNM25_06480</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>(S)-4-amino-5-oxopentanoate + tRNA(Glu) + NADP(+) = L-glutamyl-tRNA(Glu) + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12344"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="EC" id="1.2.1.70"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">Possesses an unusual extended V-shaped dimeric structure with each monomer consisting of three distinct domains arranged along a curved 'spinal' alpha-helix. The N-terminal catalytic domain specifically recognizes the glutamate moiety of the substrate. The second domain is the NADPH-binding domain, and the third C-terminal domain is responsible for dimerization.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glutamyl-tRNA reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.70" evidence="3 8"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQM2"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00316"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008883">
        <property type="term" value="F:glutamyl-tRNA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019353">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process from glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.30:FF:000001">
        <property type="entry name" value="Glutamyl-tRNA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000031">
        <property type="entry name" value="Glutamyl-tRNA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.30">
        <property type="entry name" value="Glutamyl-tRNA reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00087">
        <property type="entry name" value="Glu_tRNA_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000343">
        <property type="entry name" value="4pyrrol_synth_GluRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015895">
        <property type="entry name" value="4pyrrol_synth_GluRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036343">
        <property type="entry name" value="GluRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43013">
        <property type="entry name" value="GLUTAMYL-TRNA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43013:SF1">
        <property type="entry name" value="GLUTAMYL-TRNA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05201">
        <property type="entry name" value="GlutR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69742">
        <property type="entry name" value="Glutamyl tRNA-reductase catalytic, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0521">NADP</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 8" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamyl-tRNA reductase N-terminal" evidence="10">
        <location>
            <begin position="16"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Quinate/shikimate 5-dehydrogenase/glutamyl-tRNA reductase" evidence="9">
        <location>
            <begin position="197"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="211"/>
            <end position="216"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH33857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="361" mass="40008" checksum="88814A0C9C799C90" modified="2018-07-18" version="1">MNDLSKISFDVMNARVTFKNVPIHTLSKFSFKDVNAACVEFKKIPGVEECVIIQSATRVEIFTVSNVETDDSPDARRPEGKVLVLNQIKDTWISLSSLEQIDIDHFDQTLEVYKGDDVYLNLLRLTSGLDALVVGWQNVFDDVVNAITNAKNAGTSGPILNKLFESVIRLSTRMRDTTGISKDVVSLGDVAVKLVDEKAGLDSKKKVLLIGTGESAATLVKALDKKGISFDVTSRTLERATGFTTILGGNPIDFNDVLTGFDKYDIVFVATTSDYFLITYDRIRLVMEEKTKGTLILDLSEPRTVDEGITALPGIKLLFRDQIAEIYEESVRTRTGIVPAVEKIIEKELPVLSARMKRFDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQN6</accession>
    <name>A0A2S2KQN6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Succinate--CoA ligase [ADP-forming] subunit alpha</fullName>
            <ecNumber evidence="4">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Succinyl-CoA synthetase subunit alpha</fullName>
            <shortName evidence="4">SCS-alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">sucD</name>
        <name evidence="7" type="ORF">NZNM25_07730</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>GTP + succinate + CoA = succinyl-CoA + GDP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:22120"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>succinate + ATP + CoA = succinyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="4"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876630.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQN6"/>
    <dbReference type="GeneID" id="76208902"/>
    <dbReference type="OrthoDB" id="55711at2157"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009361">
        <property type="term" value="C:succinate-CoA ligase complex (ADP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004776">
        <property type="term" value="F:succinate-CoA ligase (GDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000006">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000277">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01988">
        <property type="entry name" value="Succ_CoA_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005810">
        <property type="entry name" value="CoA_lig_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117:SF2">
        <property type="entry name" value="SUCCINATE--COA LIGASE [ADP_GDP-FORMING] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117">
        <property type="entry name" value="SUCCINYL-COA LIGASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001553">
        <property type="entry name" value="SucCS_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01798">
        <property type="entry name" value="SCOASYNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="CoA-binding" evidence="6">
        <location>
            <begin position="16"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="4 5">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="28"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit beta</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001553-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="305" mass="32017" checksum="7CA9009FD0B1A919" modified="2018-07-18" version="1">MTDIFGLLKGKPGEPDYEKKGVIVQGITGSYGSLHAANMIAYGTNVVAGVTPGKGGQAWNDSVPIYNTMQEAVDKTNAKISIIFVPAKFFLGAAKEALEAGIKLLVAIPEHVPIRDTMETLELANKKGAVVIGPNTPGIMIPELIKIGIMPPMPFKAGKIAVLSKSGTLLYEISDALTNAGFGQSITIGIGGDPINGTRLIDAFEMVKDIPDLEGMVVVGEIGGDSEEILAQRIIDSGFNKPTVAYIAGRAAPKEKRMGHAGAIVMGTYGSAESKISMFNKANIPVAKRPAEVPVLLAGKMEKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQP1</accession>
    <name>A0A2S2KQP1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_05560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829041.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQP1"/>
    <dbReference type="GeneID" id="76209906"/>
    <dbReference type="OrthoDB" id="3147at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="58" mass="6083" checksum="07108DF90D87452D" modified="2018-07-18" version="1">MVIVWIGIAVGILIVAILIIKATKTNPREIAGMTMVCKKCGTETGGMACPKCTKTFGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQP8</accession>
    <name>A0A2S2KQP8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07700</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876628.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQP8"/>
    <dbReference type="GeneID" id="76208907"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="338" mass="40156" checksum="0AA95DC29D455464" modified="2018-07-18" version="1">MNIFFDQYKRFIERIGIQYPEPTSTAFRERKFDKVKWSKPEHRNFFTIIKILAERGPSTINEIVESDGLSQQFKPKNSRYITYRRIIVGDKKTNVTGLIEKGAVTPSKAEDKLHKKYELSHNGILYAIKLFMDLEIVYSGNYKNMLEMDSNVKWYDYSKQKEFPTTIMDIVAKNYSHRLPLIFGKWDYLKNNPRVNVYNLYDLTIIKPDSKMLLSDSISGNTKYSITFNTFDGDITLGFYTRQIESAYYSIQHFVKAIDDEDIKDFLDKMFYSYERLHRQNYYFSQAHYFLYKGQKEKAVKYMIKGVESNDMLSKQEKEKFKKKSLDRLDYLGITLCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQQ8</accession>
    <name>A0A2S2KQQ8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pyruvoyl-dependent arginine decarboxylase</fullName>
            <shortName evidence="6">PvlArgDC</shortName>
            <ecNumber evidence="6">4.1.1.19</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="6">Pyruvoyl-dependent arginine decarboxylase subunit beta</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="6">Pyruvoyl-dependent arginine decarboxylase subunit alpha</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="6" type="primary">pdaD</name>
        <name evidence="7" type="ORF">NZNM25_08110</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>L-arginine + H(+) = agmatine + CO2</text>
            <dbReference type="Rhea" id="RHEA:17641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:58145"/>
            <dbReference type="EC" id="4.1.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="6">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the PdaD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.19" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876661.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQQ8"/>
    <dbReference type="GeneID" id="76208863"/>
    <dbReference type="OrthoDB" id="30748at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008792">
        <property type="term" value="F:arginine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006527">
        <property type="term" value="P:L-arginine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.20.10">
        <property type="entry name" value="Pyruvoyl-Dependent Histidine Decarboxylase, subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01404">
        <property type="entry name" value="PvlArgDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016104">
        <property type="entry name" value="Pyr-dep_his/arg-deCO2ase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016105">
        <property type="entry name" value="Pyr-dep_his/arg-deCO2ase_sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002724">
        <property type="entry name" value="Pyruvoyl-dep_arg_deCO2ase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40438">
        <property type="entry name" value="PYRUVOYL-DEPENDENT ARGININE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40438:SF1">
        <property type="entry name" value="PYRUVOYL-DEPENDENT ARGININE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01862">
        <property type="entry name" value="PvlArgDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005216">
        <property type="entry name" value="Pyruvoyl-dep_arg_deCO2ase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01170">
        <property type="entry name" value="Pyruvoyl-dependent_arginine_de"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00055">
        <property type="entry name" value="Pyruvoyl-Dependent_Histidine/A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56271">
        <property type="entry name" value="Pyruvoyl-dependent histidine and arginine decarboxylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 6" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_5023397090" description="Pyruvoyl-dependent arginine decarboxylase subunit beta" evidence="6">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023397091" description="Pyruvoyl-dependent arginine decarboxylase subunit alpha" evidence="6">
        <location>
            <begin position="44"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic)" evidence="6">
        <location>
            <begin position="43"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser)" evidence="6">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="183" mass="20307" checksum="4E64EC77C0A52AF8" modified="2018-07-18" version="1">MLDLVAKKLFLTKGKGVHEDRLTSFEYALRDAGIAGTNLVLISSIFPPKAKLISRKEGLKQISPGQILFTIYSKNQTREPYRMCAASVGIALPKDNSRYGYLSEYESFGQNEAQAGDYAEDIAAQMLASSLGIPFDVDKNWDEKRQQWSISGKIYNTKNITQSTKGDKDGKWTTVFAAAVLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQR3</accession>
    <name>A0A2S2KQR3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aldo/keto reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_06880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876526.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQR3"/>
    <dbReference type="GeneID" id="76210049"/>
    <dbReference type="OrthoDB" id="7236at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19101">
        <property type="entry name" value="AKR_unchar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43147:SF2">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43147">
        <property type="entry name" value="PROTEIN TAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="1">
        <location>
            <begin position="16"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="328" mass="37330" checksum="5B9934776979EFD0" modified="2018-07-18" version="1">MTIEKITIGEDLEICRILNGMWQVAGGHGQIDKELAIADMIEYHKSGFTTWDLADIYGPAESLIGEFQEKIDKTQSQALTKFVPNPGPMSNSIVTYHIEQSLKKMKTECIDLLQFHWWDYNDSSYLDALNHLSKLQKDGKIKHLGLTNFDTKRVKIMIENDFQIVSNQVQYSILDQRPEKIMTPFFAKHGIKILTYGTLLGGFFSEKYLGLDEPHRGELTTSSLQKYKNMIDVWGGWNLFQELLCALDQISKKHGCSIANVATRFVLDRPQVTGVIIGARLGINNHRNDNSKVFDIKLDNDDISMINAITEKSNDLFEIIGDCGDEYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQR6</accession>
    <name>A0A2S2KQR6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07940</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876648.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQR6"/>
    <dbReference type="GeneID" id="76208882"/>
    <dbReference type="OrthoDB" id="10401at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="487" mass="53396" checksum="A468F05C21EBE21F" modified="2018-07-18" version="1">MRTLRTQLSALTVILFTLGFVPYLTSYAFAAEPEFSSFVADDPDDLDAILSNGDTLTITFSEATNATNNGAMTRAEIIANFTDGTGGVDWGDAYSGVWNGDSTVLTVTLTDVTNAILTLGATTIEGRSTTNISSAAEGTNAELLDATSVTATLSGDFGLFVAVSASNGSGCSGDCEEPTLGLDSKGERLVDNGFTYNGKPIDVERYFTPYPLVTVNTGEKNIAEFKIYDNGGPDNISHFEIAFGLAKGEIMSESRAVINWDKSFDGEKTLSVNDPEHVLGDIEVSTSEGNCSEESAQQCLLVKVIHTFRAPLDFNILGTNVWDTKRNAWQNYYNHGIEVVGPSMNPAKEYDGINKGQIYHLTETGKTTAVDEFGNSWSLVIDQWTMDYVPNKKIIDEISMAGIAREHAYFNMYKYGQYLLAEHELNQICPECLDDSYVEIDNIFAYDFQERVNKLDKPVVKQKLLLGSEMAQKTMNHILDPISHLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQS0</accession>
    <name>A0A2S2KQS0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cation transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_05860</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQS0"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="entry name" value="Cation efflux protein, cytoplasmic domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050291">
        <property type="entry name" value="CDF_Transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840:SF15">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="Cation efflux protein cytoplasmic domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="7">
        <location>
            <begin position="18"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein cytoplasmic" evidence="8">
        <location>
            <begin position="216"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein cytoplasmic" evidence="8">
        <location>
            <begin position="300"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein cytoplasmic" evidence="8">
        <location>
            <begin position="384"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="459" mass="51111" checksum="E2207F6E24167598" modified="2018-07-18" version="1">MIRIIKNKKMFVQRTKVLQISLLAIFSAFLVELIFGLISNSLALITDSIHALLDSVVTVVLLLAARMAMKPADAEHTYGHGKIESLGGMIGGIAIFLIACFFVYESINRLQSPPPTILPGLFAIVAGLYTIGIDIFRLILLRNSIKKIGGSTLKADFYHAFMDLGSTLVAIIGIVLVTYGLYFGDFVAALILGGFLAVLSIKLVYKTALDLTDIISPELVKKVKEIAASTEGVINADPVLMRRSGDTIFADITISLRGDTSFDKAHEISNNVEKNIKNKILNSTVTVHLEPNWQDVPLDSKILEIAKRVKGVKEVHNVNTHKTKGKIFSNLHVMVDREMNLLSAHKISEIIEQEIYENIPEIEHTTIHLEPFVKIPENLNLEDKITENQIKRILEKYSEIRKIGRIMSLNFENILKIDIDCSFDGELSIEKVHDLVSEIENKIRAEISDSVITIHPEPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQT0</accession>
    <name>A0A2S2KQT0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Haloacid dehalogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_05960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876447.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQT0"/>
    <dbReference type="GeneID" id="76209948"/>
    <dbReference type="OrthoDB" id="26985at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14820">
        <property type="entry name" value="TRAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002848">
        <property type="entry name" value="Translin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036081">
        <property type="entry name" value="Translin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10741">
        <property type="entry name" value="TRANSLIN AND TRANSLIN ASSOCIATED PROTEIN X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01997">
        <property type="entry name" value="Translin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74784">
        <property type="entry name" value="Translin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR602848-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="204" mass="23460" checksum="772CF6EC04FB2F10" modified="2018-07-18" version="1">MTLKNVKISLNKISKSLVDIQDSREFLLKNTREIIILCSKAIIAVHKGELSTGKNNLKQADILLKKYKKKSTGGLEKYLITPEQEFVEAASLIAIVEKKEIPSDKKLSVMPESYVLGLLDCVGELKRMVFDKIRIGKIDEATRIFDIMENLYLQLYTFSLYDKVVKEARRKIDVNRILVDDVRSAITEEKRRSELIKTLQKLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQU2</accession>
    <name>A0A2S2KQU2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_06920</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQU2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="74" mass="8569" checksum="F4A3C9FC3117BA78" modified="2018-07-18" version="1">MDVSTVKRKPHIPKKEKTRTITYRLSEKLVAELENEAMQKGISHNVLMNQIAEKYIQWDRFGDKIGMIPVPKGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQV1</accession>
    <name>A0A2S2KQV1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator AsnC/Lrp ligand binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQV1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="1">
        <location>
            <begin position="4"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="71" mass="8122" checksum="4689F4309B0D032D" modified="2018-07-18" version="1">MLISCEIGEEQLLYSQLKEIPEVKSCLITYGSYDIVAEFTTDTPAQMNEVITTKIRKLQKIRSTITLRVIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR00</accession>
    <name>A0A2S2KR00_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Succinate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_06660</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876505.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR00"/>
    <dbReference type="GeneID" id="76210025"/>
    <dbReference type="OrthoDB" id="5807at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase, transmembrane subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000701">
        <property type="entry name" value="SuccDH_FuR_B_TM-su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01127">
        <property type="entry name" value="Sdh_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="Fumarate reductase respiratory complex transmembrane subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="15881" checksum="400C2C3414EAE638" modified="2018-07-18" version="1">MTEDEHKEGIKGMANPSRYGIERVAYWLMRLSGLGLLAYFVAHIYETSNILRGQVGWDEFLELTQTTEGHIFLAIVIAMCVFHTVNGIRVMLGHGGIGVGKPARPDYPYDPASQNYRHKIGIYSAIVLAAIAMMYGLAVMFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR23</accession>
    <name>A0A2S2KR23_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07720</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876629.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR23"/>
    <dbReference type="GeneID" id="76208904"/>
    <dbReference type="OrthoDB" id="4971at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="82" mass="9694" checksum="497468AE80C79354" modified="2018-07-18" version="1">MSLQEFDALINRMKLAYDYADNLGQYVEAAKVLYQMNDQLPDDLQLYLEELDNPESAKSFIMKYNNELKSAIVSYRQRLMNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR31</accession>
    <name>A0A2S2KR31_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Type-5 uracil-DNA glycosylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">NZNM25_06960</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the uracil-DNA glycosylase (UDG) superfamily. Type 5 (UDGb) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876531.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR31"/>
    <dbReference type="GeneID" id="76210056"/>
    <dbReference type="OrthoDB" id="8612at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033958">
        <property type="term" value="F:DNA-deoxyinosine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10031">
        <property type="entry name" value="UDG-F5_TTUDGB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051536">
        <property type="entry name" value="UDG_Type-4/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044147">
        <property type="entry name" value="UdgB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33693">
        <property type="entry name" value="TYPE-5 URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33693:SF3">
        <property type="entry name" value="TYPE-5 URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00987">
        <property type="entry name" value="UreE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="10">
        <location>
            <begin position="46"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH33905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="219" mass="24721" checksum="91F6E918127EA790" modified="2018-07-18" version="1">MESIESLNKKIASCKKCPRLSKYIRDVAKNKVRRFKDDRYYGKPLSGFGDVNAKLLIVGLAPAAHGGNRTGRMFTGDSSGDWIAKVMYKHGFASIPTSQRIDDGLILKNAYITAAVRCAPPQNKPTQEEMNTCFGYLEKEKEILKNITTVLCLGKIAYDATCKLYKIKPEKFGHNKLFRYDKIRILTSYHPSKQNTQTGRLTWAQWSAVFAKAKKLTMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR51</accession>
    <name>A0A2S2KR51_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sulfate ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_09330</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876767.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR51"/>
    <dbReference type="GeneID" id="76208737"/>
    <dbReference type="OrthoDB" id="50379at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30151:SF0">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE PROTEIN MJ0413-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30151">
        <property type="entry name" value="ALKANE SULFONATE ABC TRANSPORTER-RELATED, MEMBRANE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="57"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="252" mass="27116" checksum="6383FF4526BE61E3" modified="2018-07-18" version="1">MAKNFTPHRIAFYIGIVAVWQIVAMTGVWPDNVFPSPYEVAEDLAYSASDGSLFYGIATSMWRLILGLAIAIAGGVVLGIFMARVEVINKTVGSLVLGLQSIPSIAWVPLAILWFGLTDGGIIFVTAIGAIFAVTINTYTGVKNIDPHFIEAARNMGAKGSQLITAVLIPAAFPYMITGFKQGWAFAWRGVIGAEILFSFLGLGFLLNAGRSLTDVSQVIAIMIIIMGIGLVIDGVVFTRLENKVMSRWGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR61</accession>
    <name>A0A2S2KR61_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Methyl-accepting transducer domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_07290</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR61"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.950">
        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033479">
        <property type="entry name" value="dCache_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051310">
        <property type="entry name" value="MCP_chemotaxis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029151">
        <property type="entry name" value="Sensor-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43531:SF11">
        <property type="entry name" value="METHYL-ACCEPTING CHEMOTAXIS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43531">
        <property type="entry name" value="PROTEIN ICFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02743">
        <property type="entry name" value="dCache_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103190">
        <property type="entry name" value="Sensory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Methyl-accepting transducer" evidence="8">
        <location>
            <begin position="29"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="8">
        <location>
            <begin position="387"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="721" mass="80360" checksum="F2AA3C56388E4CE2" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRA7</accession>
    <name>A0A2S2KRA7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08880</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_264953861.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRA7"/>
    <dbReference type="GeneID" id="76208782"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="519" mass="59140" checksum="310CAC39842B7D4F" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRB0</accession>
    <name>A0A2S2KRB0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">5-formyltetrahydrofolate cyclo-ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_07790</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5-formyltetrahydrofolate cyclo-ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876634.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRB0"/>
    <dbReference type="GeneID" id="76208896"/>
    <dbReference type="OrthoDB" id="18307at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030272">
        <property type="term" value="F:5-formyltetrahydrofolate cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009396">
        <property type="term" value="P:folic acid-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002698">
        <property type="entry name" value="FTHF_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02727">
        <property type="entry name" value="MTHFS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23407:SF1">
        <property type="entry name" value="5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23407">
        <property type="entry name" value="ATPASE INHIBITOR/5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01812">
        <property type="entry name" value="5-FTHF_cyc-lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006806">
        <property type="entry name" value="FTHF_cligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="186" mass="21410" checksum="AEFA3B09F9468D07" modified="2018-07-18" version="1">MDDNKKKALRNLLLEKRDNTSFDLLKIASEKMQKKIKKISAFKNAQKIGMYYPIQSEIFTQDMIQELISDGKEVFLPKVIGDEMEFRKIKDFSSLEKGRFDIMEPKDDCNADNSLDVIIIPAVGMSPSGVRLGYGHGFYDKFLAENKTTTIVLTLEKQIVKNIPKSEHDVIIDWIVTEDRVFQTQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRE3</accession>
    <name>A0A2S2KRE3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_09280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRE3"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="49" mass="5948" checksum="CF2D8575A9DF8065" modified="2018-07-18" version="1">MKPVKVKFNWEHPSIEENHGHTQSEWGKPILHNEKEPSVIYSYKMKWEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRE4</accession>
    <name>A0A2S2KRE4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_09750</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876804.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRE4"/>
    <dbReference type="GeneID" id="76208694"/>
    <dbReference type="OrthoDB" id="30774at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01209">
        <property type="entry name" value="Ubie_methyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="25432" checksum="4AB9AC763CE1C356" modified="2018-07-18" version="1">MGLGSYWGEVMDVLREIIPVYDKVNSIISLGKDAEHRNRGISGRVLPGNKILDAGSGFGNMSKTAMKLTDGKISITLYDPLVPMLKNTGKHFDKSPDMANGVFEHIPFRDEEFDAVLCGYSLRDAINLRIAISEIHRVLKNDGRFVIVDLGKPDEAFIRAGVSFYLRCILPILAFVAGGRLGLKFGTLYGTFKRWPANKKLEALLLEKFSRVEFEKDLMGGAIMVAAYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRE9</accession>
    <name>A0A2S2KRE9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_10140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876835.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRE9"/>
    <dbReference type="GeneID" id="76208653"/>
    <dbReference type="OrthoDB" id="381816at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="246" mass="26697" checksum="7AA68E767431439B" modified="2018-07-18" version="1">MKTKLLIVIAVFLVMVPWIRGLEFGMNDQVMADGITMSSIIVMILAIVFSLVSWILFSWASKNIPLIGIPLSIITGASLLIPFGQVLGPMAGIIVGVVAGFAAFMLQKKITYPAQNTSMIIAAVTIAATYLVLTLMILAIQTPSHVWNTGDGIGAWTGTAQGMEEKGFDNIFNNHIGFSYFLALIPSLIITGLIIQDKIAKSKLLIITGSILMVSALLYVFVMSASGQCYLPLTFCTNFYESNYWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRJ3</accession>
    <name>A0A2S2KRJ3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876705.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRJ3"/>
    <dbReference type="GeneID" id="76208812"/>
    <dbReference type="OrthoDB" id="373199at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="72" mass="8322" checksum="D017F7B65B5A427C" modified="2018-07-18" version="1">MNKDELESKCKGSSLTSEDIQQIFQNQEDATKYRQLKEKMKQVEDESYLLDRLDACDTSGAVEVLKEFLSDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRK8</accession>
    <name>A0A2S2KRK8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_10740</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876929.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRK8"/>
    <dbReference type="GeneID" id="76208585"/>
    <dbReference type="OrthoDB" id="7874at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="94" mass="10403" checksum="2AE598ABD0A99347" modified="2018-07-18" version="1">MDLKVHINNIHGSQMAAKITGKFTVDGNEFRFNAIAFGRIGGQNIGAKLSKNTEKELEKLGYDVEEVILVLQKNLLQGDLTLPEGLKKESFVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRQ4</accession>
    <name>A0A2S2KRQ4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829074.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRQ4"/>
    <dbReference type="GeneID" id="76208533"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="46" mass="5470" checksum="BE3A09D4AE85EC53" modified="2018-07-18" version="1">MKTEKEIEEYRKEIEKRLVECGSMDAMKYYQGVLRALDWTITGIDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRQ5</accession>
    <name>A0A2S2KRQ5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein SirB1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_10380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876857.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRQ5"/>
    <dbReference type="GeneID" id="76208627"/>
    <dbReference type="OrthoDB" id="8627at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032698">
        <property type="entry name" value="SirB1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31350:SF21">
        <property type="entry name" value="F-BOX ONLY PROTEIN 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31350">
        <property type="entry name" value="SI:DKEY-261L7.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13371">
        <property type="entry name" value="TPR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13369">
        <property type="entry name" value="Transglut_core2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein SirB1 N-terminal" evidence="1">
        <location>
            <begin position="43"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="272" mass="31566" checksum="C71FB9B21E1E1898" modified="2018-07-18" version="1">MEERFDPLVAEWFAFVKNPNFNLVEKCLKFAQILEYPELNVDEYIEKINRIGMSLKESINDVKNPTYLISMLNEHLFENLGFSGDDDDYYNPKNNFLNEVIDKKSGIPITLSILYAEVAKFVGLDLKIVGFPGHVLVKYNEEMILDPFYDGRLVDVDDLQEILDVNFGGELEFQPEYLDEVKPEQILVRMTRNLKNSYVQSFVYDKALRCVNMVLAIEPESAEDIRDKGILEERLLNPDTALKYLNKYLEINPNAEDVDFVLELIRSIKSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRT7</accession>
    <name>A0A2S2KRT7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">tRNA-dihydrouridine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">dusB</name>
        <name evidence="6" type="ORF">NZNM25_11810</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRT7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017150">
        <property type="term" value="F:tRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001269">
        <property type="entry name" value="DUS_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082:SF25">
        <property type="entry name" value="DUS-LIKE FMN-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082">
        <property type="entry name" value="TRNA-DIHYDROURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006621">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="5">
        <location>
            <begin position="1"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="307" mass="34575" checksum="88789159E3B51376" modified="2018-07-18" version="1">MAGVSDPALRLQCKKMGAGLVVTEFTNIHSIIAKEKQLKENMQTIQNFIEYSEEERPLSIQLFGSDLVALEKAAKIVEPYFDIIDYNMGCPAPHITQQMAGGALLQEVNLTQQIFSTLVNAVKKPVTLKIRSGVTNASQFLFRDIAEIAEDEGIQMITLHPRTVSQGYSGSADWKMIKELKEISNIPIVGNGDITTPEDAKNMIDETGCDYVMIGRGAMGNPFLFEQINDYFKTNSYHEYSFKDRLDSFFDYLHLTVRYKIKFANIKSQAMRFTKGMKGGSKLRSKITISKNIAELEKIMTDAYFES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRV4</accession>
    <name>A0A2S2KRV4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pyridoxal 5'-phosphate synthase subunit PdxT</fullName>
            <ecNumber evidence="7">4.3.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pdx2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Pyridoxal 5'-phosphate synthase glutaminase subunit</fullName>
            <ecNumber evidence="7">3.5.1.2</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pdxT</name>
        <name evidence="10" type="ORF">NZNM25_11350</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + phosphate + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the glutaminase PdxT/SNO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="7"/>
    <dbReference type="EC" id="3.5.1.2" evidence="7"/>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876979.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRV4"/>
    <dbReference type="GeneID" id="76208522"/>
    <dbReference type="OrthoDB" id="26717at2157"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903600">
        <property type="term" value="C:glutaminase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006543">
        <property type="term" value="P:L-glutamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008614">
        <property type="term" value="P:pyridoxine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01749">
        <property type="entry name" value="GATase1_PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000041">
        <property type="entry name" value="Glutamine amidotransferase subunit pdxT, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01615">
        <property type="entry name" value="PdxT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002161">
        <property type="entry name" value="PdxT/SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021196">
        <property type="entry name" value="PdxT/SNO_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03800">
        <property type="entry name" value="PLP_synth_Pdx2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01174">
        <property type="entry name" value="SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005639">
        <property type="entry name" value="Glut_amidoT_SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51274">
        <property type="entry name" value="GATASE_COBBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01236">
        <property type="entry name" value="PDXT_SNO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51130">
        <property type="entry name" value="PDXT_SNO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="7 8">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <begin position="54"/>
            <end position="56"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <begin position="147"/>
            <end position="148"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR005639-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR005639-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="205" mass="22037" checksum="0D0C2396ED3A758A" modified="2018-07-18" version="1">MSLNVGVLSIQGDVQENLLSVKAAIDELGIDGKVLGVKTPEEISKLDGLIIPGGESTTIGQLSLVNGSLKVLKEKIENGMPVLGICAGMIMLSNSASDRIIGETDQPLLNILDIKLERNSFGRQKESFEADVSLDSIGIPKFTGVFIRAPSVSDVGSDVEILSKFNDRIIAVKKGNVIGTSFHPELTKDFSLHKYFINLVKSLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRV8</accession>
    <name>A0A2S2KRV8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF35 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11930</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRV8"/>
    <dbReference type="OrthoDB" id="9573at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.30.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="102" mass="11842" checksum="B51A9BE9A0D71E8C" modified="2018-07-18" version="1">MNFESELHNGNFYIPECIKCLKVVWPPSEFCNHCFGEISLKKKAHEGKIIEFSRQDDMYFCLVEFDEEIKVMAKIPDTPKIGQTVKILKCGISNGNYFFQVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRW0</accession>
    <name>A0A2S2KRW0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 11 12">Adenine phosphoribosyltransferase</fullName>
            <shortName evidence="11">APRT</shortName>
            <ecNumber evidence="6 11 12">2.4.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 14" type="primary">apt</name>
        <name evidence="14" type="ORF">NZNM25_10720</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 11">Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>AMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:16609"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.4.2.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 11">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 11">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.7" evidence="6 11 12"/>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876928.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRW0"/>
    <dbReference type="GeneID" id="76208587"/>
    <dbReference type="OrthoDB" id="8323at2157"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00646"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002055">
        <property type="term" value="F:adenine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003999">
        <property type="term" value="F:adenine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006168">
        <property type="term" value="P:adenine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000021">
        <property type="entry name" value="Adenine phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00004">
        <property type="entry name" value="Aden_phosphoribosyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005764">
        <property type="entry name" value="Ade_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050054">
        <property type="entry name" value="UPRTase/APRTase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01090">
        <property type="entry name" value="apt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002634">
        <property type="entry name" value="PRK02304.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002636">
        <property type="entry name" value="PRK02304.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315:SF3">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 11" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="13">
        <location>
            <begin position="27"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="NCBIfam" id="TIGR01090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GBH34281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="170" mass="18860" checksum="6C3369D058BADCB6" modified="2018-07-18" version="1">MNLKDKIAEYPNFPKKGILFRDFSPILKDPSALTEIADEFSKYFHTKDIDVFAGIESRGFILACILASRYNKGMMMIRKAGKLPGKTVKLSYTIEYGKDTIEIQKDIIEKGQRVLICDDLLATGGTAKASAKLIEKVGGKVSGFAFIIELTELNGMKGISSYNCKSLVKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRW7</accession>
    <name>A0A2S2KRW7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Type II methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.113</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">N-4 cytosine-specific methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_09790</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-methyl-2'-deoxycytidine in DNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16857"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:13674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:137933"/>
            <dbReference type="EC" id="2.1.1.113"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the N(4)/N(6)-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.113" evidence="4"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829059.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRW7"/>
    <dbReference type="GeneID" id="76208690"/>
    <dbReference type="OrthoDB" id="38200at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015667">
        <property type="term" value="F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001091">
        <property type="entry name" value="RM_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370">
        <property type="entry name" value="RNA METHYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370:SF3">
        <property type="entry name" value="TRNA (GUANINE(10)-N2)-METHYLTRANSFERASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00508">
        <property type="entry name" value="S21N4MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0680">Restriction system</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA methylase N-4/N-6" evidence="5">
        <location>
            <begin position="26"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="258" mass="30525" checksum="43C930F3321B4F07" modified="2018-07-18" version="1">MKKIEINNIYNLNCIEGMNLIPKNKIDLVITDPPFAINFKANKANYNRTSSRVLTGYNEIKPGDYYDFTFAWMSEVFRILKDSGSMYVFSGWNNLKDILRALDDVGFTTVNHIIWKYQFGVVTKKKFVTSHYHCLYVCKDNKKRKFYPFSRFQKNDKTKDGRSLHYKDKEDVWDIKREYWTGDEKTPTKLPSEIIKKLLEYSSEKKDVVFDPFLGSGQVAVVSKSLNRRYLGFEIVSDYYKFAKKRLDKNMYRIKTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS27</accession>
    <name>A0A2S2KS27_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">HIT family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_12440</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877087.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS27"/>
    <dbReference type="GeneID" id="76209260"/>
    <dbReference type="OrthoDB" id="26806at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047627">
        <property type="term" value="F:adenylylsulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009150">
        <property type="term" value="P:purine ribonucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006790">
        <property type="term" value="P:sulfur compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47670">
        <property type="entry name" value="ADENYLYLSULFATASE HINT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47670:SF1">
        <property type="entry name" value="ADENYLYLSULFATASE HINT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="4">
        <location>
            <begin position="4"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="2 3">
        <location>
            <begin position="96"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="1">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="135" mass="15159" checksum="3B5D991821DC1E18" modified="2018-07-18" version="1">MNCIFCEILDGKRAGHLLYEDDSHIAFLDKYPIDVGHSLIIPRTHHERITDMDSEDVGKIFSLVPKIARAILDATGADAFSLGQNNGRAAKQIIPHVHIHIIPRYNSKGTVWTKRQISSDAELVELSKNIRSFIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS36</accession>
    <name>A0A2S2KS36_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877097.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS36"/>
    <dbReference type="GeneID" id="76209245"/>
    <dbReference type="OrthoDB" id="2132at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="90" mass="10364" checksum="15226392D56D046C" modified="2018-07-18" version="1">MTLVDKFQNKLRCSCNDVVLFDIIDDIECDWGNHVVIQCPNCEELFSIDNQCSAFQNISKLIDCNMGLYSKDELTNYLSNSHPTELFGHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS41</accession>
    <name>A0A2S2KS41_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ribonuclease</fullName>
            <ecNumber evidence="12">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">NZNM25_10660</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 12">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the RNase HII family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="12"/>
    <dbReference type="EMBL" id="BGKI01000005">
        <property type="protein sequence ID" value="GBH34275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876922.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS41"/>
    <dbReference type="GeneID" id="76208594"/>
    <dbReference type="OrthoDB" id="33866at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032299">
        <property type="term" value="C:ribonuclease H2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07180">
        <property type="entry name" value="RNase_HII_archaea_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.460">
        <property type="entry name" value="Ribonuclease hii. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004649">
        <property type="entry name" value="RNase_H2_suA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001352">
        <property type="entry name" value="RNase_HII/HIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024567">
        <property type="entry name" value="RNase_HII/HIII_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023160">
        <property type="entry name" value="RNase_HII_hlx-loop-hlx_cap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00729">
        <property type="entry name" value="ribonuclease HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954:SF23">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01351">
        <property type="entry name" value="RNase_HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51975">
        <property type="entry name" value="RNASE_H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 12" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="10 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-2" evidence="13">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GBH34275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="206" mass="22915" checksum="F6B557132AF16056" modified="2018-07-18" version="1">MQICGVDDAGRGSMLGPLVIAGISLDKKNLQKLSSLGVKDSKKLSPKLREQFYKKIIDIADDYYIVKISPRLIDASVKKHCLNGLEAKYMAKVVSKLNADISYVDSCDVNPARFGKEISKQSDNRKVKSYHHADSRFVIVSAASILAKVTRDKAIAKLRQNHDLGSGYPSDTVTVKFVTKYHVTYSVLPNFVRKSWKPVQKIVGNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS42</accession>
    <name>A0A2S2KS42_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">indole-3-glycerol-phosphate synthase</fullName>
            <ecNumber evidence="3">4.1.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_12910</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23476"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="EC" id="4.1.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.48" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877119.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS42"/>
    <dbReference type="GeneID" id="76209209"/>
    <dbReference type="OrthoDB" id="15223at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00043"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004425">
        <property type="term" value="F:indole-3-glycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00331">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000024">
        <property type="entry name" value="Indole-3-glycerol phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045186">
        <property type="entry name" value="Indole-3-glycerol_P_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013798">
        <property type="entry name" value="Indole-3-glycerol_P_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22854:SF2">
        <property type="entry name" value="INDOLE-3-GLYCEROL-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22854">
        <property type="entry name" value="TRYPTOPHAN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00218">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Indole-3-glycerol phosphate synthase" evidence="9">
        <location>
            <begin position="23"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="258" mass="28928" checksum="B23FC9FE08D35050" modified="2018-07-18" version="1">MAENILRKLVNNSQMAIDDGIYDIDAKLQKSSKDFVHIIKTNPHATLLTEIKFASPSLGKIRTTSDPVSIANQMIAGGAKALSILTQPHLFNGSPEFFMKVRQAFDVPLLMKDIMIDKIQIDAAKKIGADYMLVIQSLFDQKFLKDIDEFISYGHKQGLEILLEVHTKDEFENALKTDADIIGINNRNLDTLEIDIKTTEYVLKGYEKSRIILSESGIETPEDINYLKKCGADAFLIGSSIMKSDNIQEQVKKLVNAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS63</accession>
    <name>A0A2S2KS63_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11980</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877047.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS63"/>
    <dbReference type="GeneID" id="76209308"/>
    <dbReference type="OrthoDB" id="1792at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="76" mass="8675" checksum="809DCEC5D1889E06" modified="2018-07-18" version="1">MPTDKEIAIYALGKTEGVHSIAETLGKGLDDEKYIESWKKTMNMLGIDMSLKDLEKIYDDFAMKMEEIVKSNESKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSC1</accession>
    <name>A0A2S2KSC1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSC1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="116" mass="13267" checksum="92651DF549AADB05" modified="2018-07-18" version="1">MFSQEGGIKNLRHKFINNSYRKCHMSEDPLEEIIIQVINGAVSTIPAYLQDIKENKDILKVENPQEFVYGIVMGMALGMGGAILSSQKEIPTTEDQMKIRDIVYKHIPDIRERIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSE1</accession>
    <name>A0A2S2KSE1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSE1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="82" mass="9304" checksum="029EF74AC2262DCF" modified="2018-07-18" version="1">MTVFTMSGIKAYDIKARKKVLMKNPQPYLMRNGSWALKGTSAETGIKLFKIVGRAKPSIIQSRSEVFKNIFTSRQCECEKLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSE9</accession>
    <name>A0A2S2KSE9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11760</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSE9"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="75" mass="8917" checksum="E0367D07DF276D39" modified="2018-07-18" version="1">MKLSEDRFDNEKRKLTPDTGFNLVSIDYFAEPGNQLYLVEHFEMYQDALNAKKDRKNPEEYFVLYKGAGEEFLCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSF1</accession>
    <name>A0A2S2KSF1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Poly(3-hydroxyalkanoate) polymerase subunit PhaC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">PHB synthase subunit PhaC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">phbC</name>
        <name evidence="8" type="ORF">NZNM25_14010</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Biopolymer metabolism; poly-(R)-3-hydroxybutanoate biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877213.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSF1"/>
    <dbReference type="GeneID" id="76209098"/>
    <dbReference type="OrthoDB" id="202878at2157"/>
    <dbReference type="UniPathway" id="UPA00917"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042619">
        <property type="term" value="P:poly-hydroxybutyrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051321">
        <property type="entry name" value="PHA/PHB_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010125">
        <property type="entry name" value="PHA_synth_III_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01836">
        <property type="entry name" value="PHA_synth_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36837">
        <property type="entry name" value="POLY(3-HYDROXYALKANOATE) POLYMERASE SUBUNIT PHAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36837:SF2">
        <property type="entry name" value="POLY(3-HYDROXYALKANOATE) POLYMERASE SUBUNIT PHAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0583">PHB biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="7">
        <location>
            <begin position="81"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="354" mass="40861" checksum="9F59A2905C990823" modified="2018-07-18" version="1">MQSESKIDPKIIEEILKFSKNVINAPKLVSAPDEIVLEVTPHDIVQEMDKTRLIHYRSITDVQHKTPLLISYALINRYHILDIQPEKSWVRNLLQQGFDVYMLDWGTPTSMDKYLDFDDYVNGYLDSYVEYIKNESSNEKISLQGYCTGATIATAYTSLHPESVRNYIATAPVIDGWRDTTVISNLAKHMDVDKMVEIIGNMPPEFMYYCFSVLKPFEQGIEKYVKFFKNIDNKQFVDNFLRVEKWLGDTPPIPGELFKQWIKDIYQDNLLIQNKMYIQGQRIDLKKINMPVFTQVAVGDHLVSPECSMPLHYAIGSEDKTLRMYPTGHVGMIASSLSQKKVLPELGQWLAERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSG5</accession>
    <name>A0A2S2KSG5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_12820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877114.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSG5"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005819">
        <property type="entry name" value="H1/H5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00624">
        <property type="entry name" value="HISTONEH5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="8577" checksum="A11DFCC9A1A81A99" modified="2018-07-18" version="1">MVQTKAQRSAAAKKAAITRKRNAASKAAPKRKAAAKKAAPKRKAAAKKAAPKRKAAAKKAAPKRKAAAKKAAPKRKAAAKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSI6</accession>
    <name>A0A2S2KSI6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_14070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSI6"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="77"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="34264" checksum="D3255347BC8F3FF3" modified="2018-07-18" version="1">MKISGNVDVDPAIPTIIVQIITPSGNGLAHIDSIIPKADGTFTKTIHTGGPTWNENGKYVIKISYGGNLEKSIDYENTSSSSQSTVSQSNPSLPSPEQKLDIPSDDVSDVSFTENPKMRIFGFPAFDKSPQYYIDRYNTEPQYRSWFDSQFPFYEIDAVVGYSPTHIENFPSYDASPQYYIDRYNTEPQYRSWFDSQFPEKTIYDVLGFSTHIPDWIKTYAKNWATGEISDSEFMTGLDFMLQNKIIVIPNIDYAHSTFDNVPSWFRNISHWWANDLISQQEFINSIKFLIQNDIILLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSP0</accession>
    <name>A0A2S2KSP0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_14910</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877288.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSP0"/>
    <dbReference type="GeneID" id="76209004"/>
    <dbReference type="OrthoDB" id="2668at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="163" mass="18702" checksum="282F46060E640CAA" modified="2018-07-18" version="1">MITISPKRDFSKDFQRIKEISAKIDKKLSEVKPSDADKIDKLTLDELQDLDKIVKIADFMMCKYSDKKEMSSILRHFTSVISETAVSLEDLDDEISELIISAEDSINKVKDIHAHISNKSDFKKNYHDGPDFNEEETSGINLTNFVTEINTVEYQQNSQRHTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSQ2</accession>
    <name>A0A2S2KSQ2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator AsnC/Lrp ligand binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_12760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877329.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSQ2"/>
    <dbReference type="GeneID" id="76209225"/>
    <dbReference type="OrthoDB" id="8136at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="1">
        <location>
            <begin position="5"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="83" mass="9338" checksum="BBF6BA508E3AF4AF" modified="2018-07-18" version="1">MQAYVLINCNTGSETTLISELKELPEIIEINGVWGKYDIFIKISTADPNGVEQIVKRLRNHPDIVDTYTMHVLYGQGGTIDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSU3</accession>
    <name>A0A2S2KSU3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_14850</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877283.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSU3"/>
    <dbReference type="GeneID" id="76209010"/>
    <dbReference type="OrthoDB" id="9322at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="83" mass="9464" checksum="AC79D2B468C686CD" modified="2018-07-18" version="1">MNDEQQNSEIEKIANLMVHDGISPDEQDEAKLEKYKNMIKEDCTLDDESSMKLVYETLLYRKLKNSDSGDILEKGNDFGAGFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSW6</accession>
    <name>A0A2S2KSW6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome bc complex cytochrome b subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">NZNM25_15370</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877372.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSW6"/>
    <dbReference type="GeneID" id="76208451"/>
    <dbReference type="OrthoDB" id="55795at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.810.10:FF:000011">
        <property type="entry name" value="Cytochrome b/b6 domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.810.10">
        <property type="entry name" value="Cytochrome Bc1 Complex, Chain C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005798">
        <property type="entry name" value="Cyt_b/b6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036150">
        <property type="entry name" value="Cyt_b/b6_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005797">
        <property type="entry name" value="Cyt_b/b6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027387">
        <property type="entry name" value="Cytb/b6-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016174">
        <property type="entry name" value="Di-haem_cyt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19271">
        <property type="entry name" value="CYTOCHROME B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19271:SF16">
        <property type="entry name" value="CYTOCHROME B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00032">
        <property type="entry name" value="Cytochrom_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00033">
        <property type="entry name" value="Cytochrome_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81648">
        <property type="entry name" value="a domain/subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81342">
        <property type="entry name" value="Transmembrane di-heme cytochromes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51003">
        <property type="entry name" value="CYTB_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51002">
        <property type="entry name" value="CYTB_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="42"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="395"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="425"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b/b6 N-terminal region profile" evidence="12">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b/b6 C-terminal region profile" evidence="13">
        <location>
            <begin position="244"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="452"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="479"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GBH34746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="527" mass="58956" checksum="3669928FFDFA6BE5" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSX8</accession>
    <name>A0A2S2KSX8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Aspartate--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Aspartyl-tRNA synthetase</fullName>
            <shortName evidence="9">AspRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">aspS</name>
        <name evidence="11" type="ORF">NZNM25_14720</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the attachment of L-aspartate to tRNA(Asp) in a two-step reaction: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Asp) + L-aspartate + ATP = L-aspartyl-tRNA(Asp) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9660"/>
            <dbReference type="Rhea" id="RHEA-COMP:9678"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Binds 3 Mg(2+) cations per subunit. The strongest magnesium site (Mg1) is bound to the beta- and gamma-phosphates of ATP and four water molecules complete its coordination sphere.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.12" evidence="9"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877271.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSX8"/>
    <dbReference type="GeneID" id="76209023"/>
    <dbReference type="OrthoDB" id="5908at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000038">
        <property type="entry name" value="Aspartate--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02075">
        <property type="entry name" value="Asp_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004523">
        <property type="entry name" value="Asp-tRNA_synthase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00458">
        <property type="entry name" value="aspS_nondisc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003483">
        <property type="entry name" value="PRK05159.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450:SF1">
        <property type="entry name" value="ASPARTATE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450">
        <property type="entry name" value="ASPARTYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="10">
        <location>
            <begin position="134"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Aspartate" evidence="9">
        <location>
            <begin position="189"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="210"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="218"/>
            <end position="220"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="399"/>
            <end position="402"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="428" mass="48826" checksum="768C7E0117F6081C" modified="2018-07-18" version="1">MGFVKTHDIDELTSELIGNQVILGGWVEDFRKLGKMSFITLRDVTGISQIIVKGELNDNLGEINRQSVISVKGIVQETKARDFAFEIKAEEIEVLGKAVHPLPVDPIGRVESNIDTRLNHRALDMRNQKTASIFKLRHHVLQSLRKTLSGKKFIEITTPKIIGSASEGGANLFSLDYFGKTAYLAQSPQLYKEQMTIGLERVFEISNFYRAENSHTGRHLTEFTSIDIEAAFMDYNDVMDVLESLVLEVYKFTSENCKKEQEIIGHTIEVPKSPFERITYSQCVEELQKDGEKIEFGDDLLDSHLRIIGNNHPGFFFLTDWPMKLKPFYIREKDEDPTLSRSFDLQFGYLELSSGGTRLHNPDMLKARLKEQGLDPAQFQDHLKTFDWGMPPHSGWGMGLDRLMTTLIGIDNVREVVLYPRDPDRLNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT06</accession>
    <name>A0A2S2KT06_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Type IV pilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_15020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877297.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT06"/>
    <dbReference type="GeneID" id="76208994"/>
    <dbReference type="OrthoDB" id="4763at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="15309" checksum="90796096A7C7C39C" modified="2018-07-18" version="1">MNSLPIIALIGVAILVTSTIGFGASLTNTPTFNLIGASDNNAVTTARGNITALVWTEEVSTEGVIETDEITFAVGNEDGTEAHDFQICAVIEGPIGTYSPAAGSPPACTTTGSVAAYANSTGHTIDFVTPVNVTSIVDISFSIEELDQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT20</accession>
    <name>A0A2S2KT20_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">3,4-dihydroxy-2-butanone 4-phosphate synthase</fullName>
            <shortName evidence="6">DHBP synthase</shortName>
            <ecNumber evidence="6">4.1.99.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_16110</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>D-ribulose 5-phosphate = (2S)-2-hydroxy-3-oxobutyl phosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18457"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="4.1.99.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="6">Binds 2 divalent metal cations per subunit. Magnesium or manganese.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DHBP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.12" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877448.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT20"/>
    <dbReference type="GeneID" id="76208375"/>
    <dbReference type="OrthoDB" id="25735at2157"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00399"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008686">
        <property type="term" value="F:3,4-dihydroxy-2-butanone-4-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.870.10:FF:000012">
        <property type="entry name" value="3,4-dihydroxy-2-butanone 4-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.10">
        <property type="entry name" value="DHBP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000422">
        <property type="entry name" value="DHBP_synthase_RibB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00506">
        <property type="entry name" value="ribB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327:SF46">
        <property type="entry name" value="3,4-DIHYDROXY-2-BUTANONE 4-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327">
        <property type="entry name" value="GTP CYCLOHYDROLASE II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00926">
        <property type="entry name" value="DHBP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 6" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0686">Riboflavin biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="221" mass="24423" checksum="1E3BB52AAE1AEAF5" modified="2018-07-18" version="1">MSLESALQSLKRGEFVLLFDSAGRENEIDMVVAAEFVTPEHVARMRQYAGGLLCIAIDNNFAKSLELKYMHEILADSSISNKEMIMGLAPYGDHPTFSLSVNHYQTYTGITDKDRSLTIREMANIFNVENKQKKFASSFKTPGHVPLLIASKGLLAARQGHTEMSVYLAQVAGLTPVTAICEMMDAETYSALSVDKAEKFAKQNGIPLIDGKELLEYAKVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT48</accession>
    <name>A0A2S2KT48_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GTPase HflX</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">GTP-binding protein HflX</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hflX</name>
        <name evidence="9" type="ORF">NZNM25_16000</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">GTPase that associates with the 50S ribosomal subunit and may have a role during protein synthesis or ribosome biogenesis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer. Associates with the 50S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="5">May associate with membranes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. HflX GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877438.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT48"/>
    <dbReference type="GeneID" id="76208386"/>
    <dbReference type="OrthoDB" id="10150at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2860">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11060">
        <property type="entry name" value="GTPase HflX, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00900">
        <property type="entry name" value="GTPase_HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030394">
        <property type="entry name" value="G_HFLX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032305">
        <property type="entry name" value="GTP-bd_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016496">
        <property type="entry name" value="GTPase_HflX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025121">
        <property type="entry name" value="GTPase_HflX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042108">
        <property type="entry name" value="GTPase_HflX_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03156">
        <property type="entry name" value="GTP_HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10229">
        <property type="entry name" value="GTP-BINDING PROTEIN HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10229:SF8">
        <property type="entry name" value="GTPASE HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16360">
        <property type="entry name" value="GTP-bdg_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13167">
        <property type="entry name" value="GTP-bdg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006809">
        <property type="entry name" value="GTP-binding_hflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51705">
        <property type="entry name" value="G_HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hflx-type G" evidence="8">
        <location>
            <begin position="184"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="190"/>
            <end position="197"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="215"/>
            <end position="219"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="236"/>
            <end position="239"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="304"/>
            <end position="307"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="333"/>
            <end position="335"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006809-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR006809-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="373" mass="42498" checksum="9B4252F963E9E6FB" modified="2018-07-18" version="1">MKSAILITYDQEDSINEAKGLCDAAGYEVVHIIQQDYLQKPKYGISGGVLERLEEIADKLRPEVIIFDEILKPSQNYNLASALHREILDREGLILEIFESRASSAESKLQVKLAQLRYEMARAKEKVRLANMGEQPGFMGIGKFEVDVYYNDIKHRMQTLRSKLEKAGKQRELHRQGRKRLGFKTISLAGYTSAGKTTLFNKVTGESRAQSKELFTTLTTTTRRLTINQEPFLIADTVGFISKLPAYMIDAFKSTLEELTYTDIILLVIDISDSVFELKKKFSSCMRTLSELGVEQDKIIYVLNKDDLLKHDEINRKINILNLAENKKVISVSAKTGKNVNELKKLIKNITMSQNSYNFNRNSFEGVKETFDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT49</accession>
    <name>A0A2S2KT49_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cupin type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_14260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877233.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT49"/>
    <dbReference type="GeneID" id="76209069"/>
    <dbReference type="OrthoDB" id="190812at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02214">
        <property type="entry name" value="cupin_MJ1618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052044">
        <property type="entry name" value="PKS_Associated_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36114">
        <property type="entry name" value="16.7 KDA PROTEIN IN WHIE LOCUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36114:SF1">
        <property type="entry name" value="16.7 KDA PROTEIN IN WHIE LOCUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-2" evidence="1">
        <location>
            <begin position="40"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="120" mass="13578" checksum="6955F283E8376128" modified="2018-07-18" version="1">MSLRKNSEIQPIDGNEGTKIKQYFHPHNTLSGINYSVAQFTLKPGKSSKLHKLASSEIYYILDGNARLNVDEEAYELEKDDSAYVAPNSKQCIKNTGSENLRFLCIVEPAWKAENEEILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT65</accession>
    <name>A0A2S2KT65_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rieske domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_15380</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877373.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT65"/>
    <dbReference type="GeneID" id="76208450"/>
    <dbReference type="OrthoDB" id="5623at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014349">
        <property type="entry name" value="Rieske_Fe-S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019546">
        <property type="entry name" value="TAT_signal_bac_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01409">
        <property type="entry name" value="TAT_signal_seq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10134">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT RIESKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="6">
        <location>
            <begin position="92"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="201" mass="21978" checksum="9735C20D9C3B52BA" modified="2018-07-18" version="1">MSELGTKKSDGLSRRDFLKLMGAAGTGLAFAPFVPFGNFMPNPNQASLEKVPVILPDGTQANINTYPINHAEVITYPSTGDAALDAEAFRKWQFIRLPEELGGRKKDTSSLRAYSMICLHLWCLWKYWPDEGRKRGECPCHGSMYDPVTGTAFVGPASVQAAPSNTLAQLTLEIDSDGFVFILPPKFNANDNGVIGYGRFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT98</accession>
    <name>A0A2S2KT98_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sulfurtransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_16630</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877488.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT98"/>
    <dbReference type="GeneID" id="76208322"/>
    <dbReference type="OrthoDB" id="10492at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01448">
        <property type="entry name" value="TST_Repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01449">
        <property type="entry name" value="TST_Repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051126">
        <property type="entry name" value="Thiosulfate_sulfurtransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43855">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43855:SF1">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="13"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="149"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="258" mass="29308" checksum="C0547B7292FEB689" modified="2018-07-18" version="1">MLITTTDLNSIINDPDVIIADTRSFKEYSEEHIPGSVHLDLFAFHWIDTTKQGIENFNNQTKNLLSFLGVTPEKKIIFYDSVSGMLAARGVWMLMYFSHENTSMLDGGITKWKKENLPLETKSNGFKPSHFSGEINPEIISGFEYIRDNLKNVKILDARSPGEFDGSIVRAAQSGHIPNSINIDWNQNLNEDGTFKNDEQLLQIYNYPKDTEIITYCQGAYRAANSFLVLKKLGFKNVKVYLGSWGEWGNKLELPAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTA8</accession>
    <name>A0A2S2KTA8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable thymidylate kinase</fullName>
            <ecNumber evidence="7">2.7.4.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">dTMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tmk</name>
        <name evidence="9" type="ORF">NZNM25_16730</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>dTMP + ATP = dTDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:13517"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58369"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the thymidylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="7"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877498.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTA8"/>
    <dbReference type="GeneID" id="76208312"/>
    <dbReference type="OrthoDB" id="43083at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:dTMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006227">
        <property type="term" value="P:dUDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01672">
        <property type="entry name" value="TMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00041">
        <property type="entry name" value="DTMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344:SF1">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate kinase-like" evidence="8">
        <location>
            <begin position="5"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="193" mass="22524" checksum="6F30D20C7985B6CA" modified="2018-07-18" version="1">MIIVIEGGDQAGKLTQSTLLEKALKKRKIKTKLFHFPDYNTPIGQEIRKYLDGKRKFSPQVIHCLLSANRWEKLDEIISAQEKNSILIMNRYYHSNLIYGIANGLNPKWLENLDAGLPKADLVILLDVSQKESFDRQKTHRDKFEKNEEFLRKISKIYRTIAKKKNWKIIDASKSKQEVHEEIIKTFSKKIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTB2</accession>
    <name>A0A2S2KTB2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_15880</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877425.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTB2"/>
    <dbReference type="GeneID" id="76208399"/>
    <dbReference type="OrthoDB" id="4979at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="227" mass="27286" checksum="D3FAEA2BF7AC3C3A" modified="2018-07-18" version="1">MNIEEQIRDCEVCVKQIKQYDPDPFYVNHFFNGFINSVSNTYDGIFEEANRDFGLFISEKISLEKFHEKARKKNDQNAIKFFQWFLQKYEYEHGNPYPNFIKKICQCKIKSERIPKIKIMIRASDRYEDDINQQIKVNLSNEKLRSKEELDIEVKRQLPVFLEIINHKRNEKNEPKVGKNQINVSTFLDIENHKDIEIAYAAEIYIPVMRRLVEESRKKIKELTTWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTB8</accession>
    <name>A0A2S2KTB8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ADP-ribose pyrophosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">nudF</name>
        <name evidence="4" type="ORF">NZNM25_16850</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877508.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTB8"/>
    <dbReference type="GeneID" id="76208301"/>
    <dbReference type="OrthoDB" id="104705at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006753">
        <property type="term" value="P:nucleoside phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019693">
        <property type="term" value="P:ribose phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03424">
        <property type="entry name" value="NUDIX_ADPRase_Nudt5_UGPPase_Nudt14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839:SF18">
        <property type="entry name" value="NUDIX HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839">
        <property type="entry name" value="UDP/ADP-SUGAR PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="3">
        <location>
            <begin position="31"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="171" mass="19459" checksum="05BA2E921DEA09F8" modified="2018-07-18" version="1">MKKKKIYEGKILGLSVYDGKIEGRKVKREMIEHRGAAAMLAFDDDGKIILVKQHRFPHGYVLEIPAGTLEKKEEPIKCAYRELEEETGYRAKKMTSLITYYPSIGYNTEVIHCFVASGLKKIADLKLDEDEILSVTKMDLKKLLKLIKSGKIQDSKTICAVLTYAAKKKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTD8</accession>
    <name>A0A2S2KTD8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_17050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877523.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTD8"/>
    <dbReference type="GeneID" id="76208281"/>
    <dbReference type="OrthoDB" id="10027at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117070">
        <property type="entry name" value="LEA14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="164" mass="17817" checksum="B6DB191CB2D3A6B2" modified="2018-07-18" version="1">MALQSKTTVYAAVIGVLGLMGGIVYYASLDNVDLDQVEIELTNVELIDVSSINNQAKFEVTFLVKNPSDKTFTVSVIEYNLYGDETLLGSGIYSTADIALPGRALFSSGAEIPLKNTFVLSKDDVNTEIYEAIIDNKITSFSAEGSITTQSSWSEAQKEFKTGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTG1</accession>
    <name>A0A2S2KTG1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="1">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_17100</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="1"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877528.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTG1"/>
    <dbReference type="GeneID" id="76208274"/>
    <dbReference type="OrthoDB" id="12184at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00317">
        <property type="entry name" value="cyclophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF4">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE DOMAIN AND WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="158" mass="17455" checksum="DCBE042BAD368BD8" modified="2018-07-18" version="1">MSNVNIETNFGKIAFKLLPELAPETVRNFEKLAKDGFYDGTLFHRVIPGFMIQGGDPNTKTDNKGSWGMGGPGYNIKAEFNSRSHLRGIVSMARSQDPDSAGSQFFIVTTDSAFLDRQYTVFGEVTEGMDVADKIVNLQRDGNDCPLEKAQMVHVTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTJ1</accession>
    <name>A0A2S2KTJ1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF83 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_16680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877493.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTJ1"/>
    <dbReference type="GeneID" id="76208317"/>
    <dbReference type="OrthoDB" id="10444at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051827">
        <property type="entry name" value="Cas4_exonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022765">
        <property type="entry name" value="Dna2/Cas4_DUF83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36531">
        <property type="entry name" value="CRISPR-ASSOCIATED EXONUCLEASE CAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36531:SF2">
        <property type="entry name" value="CRISPR-ASSOCIATED EXONUCLEASE CAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01930">
        <property type="entry name" value="Cas_Cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF83" evidence="1">
        <location>
            <begin position="131"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="215" mass="25135" checksum="5829934CAE19427E" modified="2018-07-18" version="1">MMGDRDFRKIIQSAVKSIGKELEVQVDSKDIQTIHLHEVVQCLRRSYYDRVDPQEIERRGFNELLSGLLRKLEYGSEPKEFAIEDIRLKGQVDMIVDDTILLFRPAPVELESPQANDVLYLNACMWIYDKNDGVVVYISGDRRETTFSLTRNKKMFEEVIRRVRVLNDLLKEQKIPILEPSTECSECQYYERCFMKKKNSKQISLAEMLGMGDNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTQ3</accession>
    <name>A0A2S2KTQ3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_18410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877640.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTQ3"/>
    <dbReference type="GeneID" id="76210280"/>
    <dbReference type="OrthoDB" id="10755at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="292" mass="34223" checksum="8D450291492445AB" modified="2018-07-18" version="1">MVINFKIIFIGIIAAFITLVIFTQYQTEIPIEESNIHDIEFFNFNIDFKDFDMVELPIDSIFIIKAIKDDYILDKNIHKYLKLAFELDDENLSLYDELSNTDEKTVVIFPIFTSSAYNSPGFYDYYSDRCDVSCLTVPIKLILRTEMGGNGAQILKLLNYKFLSDIDVDKNPEILNHFDKVILLHSEYVTKKEFDAITSHPNVIYLYPNALYAEIEVNYDQNTATLIRGHGYPEKHIDNGFDWVFDNTRPYEFDRDCDNWEFYEIENGKMLNCFPEEQLYEDASLLKALKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTS6</accession>
    <name>A0A2S2KTS6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Geranylgeranylglyceryl phosphate synthase</fullName>
            <shortName evidence="9">GGGP synthase</shortName>
            <shortName evidence="9">GGGPS</shortName>
            <ecNumber evidence="9">2.5.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">(S)-3-O-geranylgeranylglyceryl phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Phosphoglycerol geranylgeranyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_17720</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P). This reaction is the first ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>sn-glycerol 1-phosphate + (2E,6E,10E)-geranylgeranyl diphosphate = sn-3-O-(geranylgeranyl)glycerol 1-phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23404"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57677"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="EC" id="2.5.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="9">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the GGGP/HepGP synthase family. Group II subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.41" evidence="9"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877578.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTS6"/>
    <dbReference type="GeneID" id="76208213"/>
    <dbReference type="OrthoDB" id="7409at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047294">
        <property type="term" value="F:phosphoglycerol geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02812">
        <property type="entry name" value="PcrB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.390:FF:000001">
        <property type="entry name" value="Heptaprenylglyceryl phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.390">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00112">
        <property type="entry name" value="GGGP_HepGP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038597">
        <property type="entry name" value="GGGP/HepGP_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008205">
        <property type="entry name" value="GGGP_HepGP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010946">
        <property type="entry name" value="GGGP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050064">
        <property type="entry name" value="IGPS_HisA/HisF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01769">
        <property type="entry name" value="GGGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01768">
        <property type="entry name" value="GGGP-family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003198">
        <property type="entry name" value="PRK04169.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235:SF22">
        <property type="entry name" value="GERANYLGERANYLGLYCERYL PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE SUBUNIT HISF/H IGP SYNTHASE SUBUNIT HISF/H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01884">
        <property type="entry name" value="PcrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 9" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="174"/>
            <end position="180"/>
        </location>
        <ligand>
            <name>sn-glycerol 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="205"/>
            <end position="206"/>
        </location>
        <ligand>
            <name>sn-glycerol 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="227"/>
            <end position="228"/>
        </location>
        <ligand>
            <name>sn-glycerol 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="250" mass="26413" checksum="AACA821D6FEDBF4D" modified="2018-07-18" version="1">MTKNQVETFLKSELKKKNALLFVLIDSEVSNLDESAKLAKDVEKIGASAILVGGSSATDQIEMAQVVKGIKKGIKIPIILFPGNVTGVVPDADAILFSSLMNSENPYFITQAQALGAPSVLKFGLEPLPTAYLVIGEGTSAWFVGAARGIPFEKPKIAAAYALAAQFLGMRFVYLEAGSGAKSNVTPEMVKTVRHAFNGFLIVGGGIKDVKTAESLVKAGADALVIGTFLEKGGSIKKLQEIAKVIQRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTU4</accession>
    <name>A0A2S2KTU4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH arsR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_18810</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877704.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTU4"/>
    <dbReference type="GeneID" id="76210108"/>
    <dbReference type="OrthoDB" id="35765at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38600">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38600:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="23"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="100" mass="11578" checksum="E54D298C545841D1" modified="2018-07-18" version="1">MANDPDAKRLLWFVFAGSRGGLNRLKIISKLKEKPFNTNQLAKEMNLDYKAIQHHIKVLEKNNMISKVGEKYGVAYFISNFLEVNMETFEEIEAKLDKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTW5</accession>
    <name>A0A2S2KTW5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dihydroorotase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_18600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877684.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTW5"/>
    <dbReference type="GeneID" id="76210087"/>
    <dbReference type="OrthoDB" id="8791at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004038">
        <property type="term" value="F:allantoinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006145">
        <property type="term" value="P:purine nucleobase catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050138">
        <property type="entry name" value="DHOase/Allantoinase_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43668">
        <property type="entry name" value="ALLANTOINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43668:SF2">
        <property type="entry name" value="ALLANTOINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="1">
        <location>
            <begin position="52"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="463" mass="51095" checksum="C6EA254960ADAC6F" modified="2018-07-18" version="1">MTYDTVITDTHIILPQGMVDKNIIIDEGKIIGFTHDTPACDEKINGNGLISVPGPIDPHVHYGVYSPINEAAKTESHAAAIGGITTMMRMLRLGDPFSKSLQAQLDAASKNHYVDYAIHASIFTQEQINEMNLCVNKGITSFKIYMNLGGEIGHVYMDMLPDSTHLVESEVNVTDKIVEQTVKTAASLGCPVLVHAEDYESCGCGIKTAKEKNQDGLHAWSESRSPEYEAKAIKTVSKFGRDYGCVIYFVHIGSERALAQIEEERKLGTKIYVETCPHYLTLSYEKQNGYLAKVMPPIRTEKDSKAVWNALSKNFINTIGTDHVANQLKLKLGGTDVWDALAGFPGIGTSLPILLNDGVNQNKITLEQFVKFTSQNAAQIFGMYPQKGTLEKNSDADITMIDLKKEKKVTSDLFGGFSDYIVYEGRNLKGWPVKTIVRGEIIAENFEVIGKIGHGKLVERKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU05</accession>
    <name>A0A2S2KU05_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed RNA polymerase subunit Rpo11</fullName>
            <ecNumber evidence="3">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">DNA-directed RNA polymerase subunit L</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpo11</name>
        <name evidence="3" type="synonym">rpoL</name>
        <name evidence="5" type="ORF">NZNM25_17290</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal Rpo11/eukaryotic RPB11/RPC19 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877542.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU05"/>
    <dbReference type="GeneID" id="76208256"/>
    <dbReference type="OrthoDB" id="6541at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00261">
        <property type="entry name" value="RNApol_arch_Rpo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009025">
        <property type="entry name" value="RBP11-like_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022905">
        <property type="entry name" value="Rpo11-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13656">
        <property type="entry name" value="RNA_pol_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RBP11-like dimerisation" evidence="4">
        <location>
            <begin position="13"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="92" mass="10255" checksum="FA108C8D7E5ECE2D" modified="2018-07-18" version="1">MNAQVISSEPKEISLSITETDIGILYIIQHELLKGSNIDFAGVIVKHPLTNECWMRINSSTKPLGEIKKATDSAIKMAEELKQLFNSKIKVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU29</accession>
    <name>A0A2S2KU29_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PseI/NeuA/B-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_19440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877754.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU29"/>
    <dbReference type="GeneID" id="76210174"/>
    <dbReference type="OrthoDB" id="71219at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047444">
        <property type="term" value="F:N-acylneuraminate-9-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051690">
        <property type="entry name" value="PseI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013132">
        <property type="entry name" value="PseI/NeuA/B-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42966">
        <property type="entry name" value="N-ACETYLNEURAMINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42966:SF1">
        <property type="entry name" value="SIALIC ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03102">
        <property type="entry name" value="NeuB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PseI/NeuA/B-like" evidence="1">
        <location>
            <begin position="24"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="243" mass="27662" checksum="64CCE10A7D28FD41" modified="2018-07-18" version="1">MKTTIAAEIGSNWEGSISIAKKIILECKKAGADAVKFQMWRAKDLYSEDHPNWNVIKKSELTFKKADKIKKISDDIGIEFMCSVFYPEGVEFLESLNVKRYKVASRTCLFKDPYSLETLKSKAITKKPIIISMGMGGNKKKIDKIFSKNKTLFCYCISEYPLEFNKINWNQATKFDGFSDHTEGITAPILFTILKKQQNTKQILIEKHTKLSNSKGPDASTSIDTKQLSELVSHIRILEKAKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU41</accession>
    <name>A0A2S2KU41_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NodB homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_19270</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU41"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10918">
        <property type="entry name" value="CE4_NodB_like_5s_6s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051398">
        <property type="entry name" value="Polysacch_Deacetylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34216">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34216:SF3">
        <property type="entry name" value="POLY-BETA-1,6-N-ACETYL-D-GLUCOSAMINE N-DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="NodB homology" evidence="3">
        <location>
            <begin position="146"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH35136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="333" mass="38393" checksum="7908F21951AE9531" modified="2018-07-18" version="1">MQSNNLEKINSSYVGLCYHYIRQSKQTDPFPRILGTNIDEFQNHLSMLQKNYQIISLEDAMNISYGGTGFHEKSSGVLITFDDGLSDHFTAAKILYELNISAIFFIPTCILQEKLPANPIIIHYTIAVFGVEKFLKEFRTILENNKLDKKLFDIQYSKDTDNVWDTISKIKSTFKYKLGFYNSRKILLDIYKNLFCTQYKNPLDIIHLTESQIRKILEMGHYIGTHTHSHISVAATELNLADFAKEIIFPKNYLEQKFNTQVNSFSYPFGGKDDCLSSSELIKKTNEYKLAFTVKEILNTKNTSPYELGRYQPTSSDTSFKLNNIIKEIIIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUA5</accession>
    <name>A0A2S2KUA5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein pelota homolog</fullName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pelA</name>
        <name evidence="11" type="ORF">NZNM25_19870</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">May function in recognizing stalled ribosomes, interact with stem-loop structures in stalled mRNA molecules, and effect endonucleolytic cleavage of the mRNA. May play a role in the release non-functional ribosomes and degradation of damaged mRNAs. Has endoribonuclease activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">The N-terminal domain has the RNA-binding Sm fold. It harbors the endoribonuclease activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the eukaryotic release factor 1 family. Pelota subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877794.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUA5"/>
    <dbReference type="GeneID" id="76210217"/>
    <dbReference type="OrthoDB" id="31300at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071025">
        <property type="term" value="P:RNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.870:FF:000008">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.870">
        <property type="entry name" value="Pelota, domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01853">
        <property type="entry name" value="PelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038069">
        <property type="entry name" value="Pelota/DOM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023521">
        <property type="entry name" value="Pelota_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058547">
        <property type="entry name" value="Pelota_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004405">
        <property type="entry name" value="TF_pelota"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00111">
        <property type="entry name" value="pelota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853">
        <property type="entry name" value="PELOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853:SF0">
        <property type="entry name" value="PROTEIN PELOTA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26356">
        <property type="entry name" value="Pelota_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159065">
        <property type="entry name" value="Dom34/Pelota N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="10">
        <location>
            <begin position="3"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH35196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="346" mass="38817" checksum="31B215AA32479FC1" modified="2018-07-18" version="1">MITKTIDENSISVMPEDSDDLLNLRRIIKKDDKVIGDTTRVLKQDKDYSRPDKGERIKVRIALTVEKISLDDVLDRLRVGGTIYESSNEAVPHGSHHSFILKINDGITISKKKWSPIEKKLLESNSQNGFVLVAIDTADCGIARLKGTHLEFMPNIYSGSGGKRYKTNFNIEKFFEQVQQAIFSISKKEDLIIIFGPGETKKRFANFIEKSQKFKIKVVEGIDSGGEDGIYTFTKSKAMQKIMSDSKLAKASTIIDEVMLLANKKSNKFTMGFEETFKTNQMGAVESLVFSDRIIQENDEQKIMDFLNDAENKGVKIYSVDSSTDIGLRVTGLGGIVSLLRYAIET</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUE0</accession>
    <name>A0A2S2KUE0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF354 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_20020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877806.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUE0"/>
    <dbReference type="GeneID" id="76210235"/>
    <dbReference type="OrthoDB" id="185087at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007152">
        <property type="entry name" value="DUF354"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39662:SF1">
        <property type="entry name" value="DUF354 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39662">
        <property type="entry name" value="DUF354 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04007">
        <property type="entry name" value="DUF354"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005357">
        <property type="entry name" value="UCP005357"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="342" mass="38817" checksum="8502719B78DCFBFB" modified="2018-07-18" version="1">MKIWIDILTPKQLLFSEPIIEKLGKKHNILCTSRNYNEVSKLAKIRDFDLVFIGKHGGSDKKSKLRASIDRMGELSRKIDNFSPDVVISFCSPEAARISFGLGIKHIAFCDSPHASAVMRLTLPLIQKLLIPSIIPKNEFSKYGIDKKDITSYNAIDAVVTIKRKINQQRSLPFKQTNRKNILIRAEEEEAAYASKSSKIIPIIKQIVKEHEKENIVILGRYTKQIQNIQKIIGKKAKVVKMTFDGKYLLSNTDIFIGSGGTMTAESALMGIPTISYNAVPNIIENFLVKKYLVKRETNPKKISRYIKNVFESSNAVNQKRAKRVVEQMEDPIEKLNKIINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUI9</accession>
    <name>A0A2S2KUI9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_19090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUI9"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="155" mass="17800" checksum="7B06A4E1EAF21C8B" modified="2018-07-18" version="1">MLFQEAYGHSMFNSAEQFEAGYRVQVATLPEFPQIGEPSQFLVKVTKGFEYEDVDRFTMGIRVFFNGQQVDAIPPTSIEGNHWDFDYVWRQQGNHIVKIDLYDMSENGVITYTFNMGTQSPFGMIFFGAIIVGALVFTGVMIYIYMPKIFKKSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUJ6</accession>
    <name>A0A2S2KUJ6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UDP-N-acetylglucosamine 4,6-dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">capD</name>
        <name evidence="3" type="ORF">NZNM25_19460</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the polysaccharide synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877756.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUJ6"/>
    <dbReference type="GeneID" id="76210176"/>
    <dbReference type="OrthoDB" id="4907at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05237">
        <property type="entry name" value="UDP_invert_4-6DH_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003869">
        <property type="entry name" value="Polysac_CapD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051203">
        <property type="entry name" value="Polysaccharide_Synthase-Rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43318">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE 4,6-DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43318:SF2">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE 4,6-DEHYDRATASE (INVERTING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02719">
        <property type="entry name" value="Polysacc_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polysaccharide biosynthesis protein CapD-like" evidence="2">
        <location>
            <begin position="7"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="334" mass="37933" checksum="321AC2E701BF76CA" modified="2018-07-18" version="1">MFEDKKILITGGTGSLGQALTKRLLDDDVKTIRILSRNEEKQVQMENSFNDNRLRFFLGDIVDYERLKRAFEDIDIVFHTAALKHVPKIEYNPFEAIKTNVIGSQNVINACLYTNVEKAIAIGTDKAVSPLNTYGATKLLMEKLFVTANNYINPEKHFTKFISVRYGNVFGSSGSVIPKFIEQIKKNQKITITDPKMTRFSITMNEALDFILNATISGEGSEIFVPKMKAYEIMDLVEVLQNLLGDTGQEIVGIRPGEKLHEILISEDEIRQTWEYNNMYLISNPLYSLFHGKSTDEIHKGITKLENLKNYSSDISDRISKSELQEMLTKSGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUU3</accession>
    <name>A0A2S2KUU3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sm-like ribonucleoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_20090</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUU3"/>
    <dbReference type="OrthoDB" id="371816at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01726">
        <property type="entry name" value="LSm6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000090">
        <property type="entry name" value="SnRNP Sm-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016487">
        <property type="entry name" value="Lsm6/sSmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021:SF0">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F SNRNP-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006609">
        <property type="entry name" value="snRNP_SmF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="2">
        <location>
            <begin position="9"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="81" mass="9260" checksum="11F61677416E932D" modified="2018-07-18" version="1">MSQSNSAKRPLTTLQKSTKKKVTVRLKNEVEYKGKMDNVDSYMNLIMTDAEELHDGKTIANYGRVIVRGNNVLFIKLENEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNQ2</accession>
    <name>A0A2S2KNQ2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNQ2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="64" mass="7513" checksum="2934161FBD92B723" modified="2018-07-18" version="1">MYYPRLSNPKAPNEHRVLVYACPDCTDDFEKPKMFSIRRNQVEDPLETVEIEITQLQKKSIGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNT2</accession>
    <name>A0A2S2KNT2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_00630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875941.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNT2"/>
    <dbReference type="GeneID" id="76209392"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="15"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="67" mass="8043" checksum="DB7E41812412A211" modified="2018-07-18" version="1">MGKLPEKFPEYSIMYKTLSKQIKALENIKEKAQEKEAKEINLKIQNYQSELLKIKKMFPDDFFDEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNW4</accession>
    <name>A0A2S2KNW4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sec-independent protein translocase protein TatC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">tatC</name>
        <name evidence="6" type="ORF">NZNM25_00550</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Forms a complex with TatA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TatC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875935.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNW4"/>
    <dbReference type="GeneID" id="76209384"/>
    <dbReference type="OrthoDB" id="15305at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033281">
        <property type="term" value="C:TAT protein transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009977">
        <property type="term" value="F:proton motive force dependent protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002033">
        <property type="entry name" value="TatC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00945">
        <property type="entry name" value="tatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30371">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30371:SF0">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATC, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01840">
        <property type="entry name" value="TATCFAMILY"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="211"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="263" mass="29376" checksum="B2C3C770B6269F53" modified="2018-07-18" version="1">MSELDGINQHLEELRKRLLRIVLVIGIITAFILTFHAEPIQISGITMYYPSPDPLNNIAAQITNHMKTNLVPEDVRLIQTAPGQAFFAQVYIAALAGIVVGMPVIVKELVGFIKPALKENEIHVSRNITIPALGLFITGCVFSYNLVIPYILDFLYRYGESAGLVTFLNVIEFVTFVLQFLLAFGFSFQLPLVMYAISVSGMVDSDFWRKNIRYAIVIITIFGAVITPDGSGVTMWFIAGPMIALYLIGMIVIERKERKKLNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP06</accession>
    <name>A0A2S2KP06_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3227 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_02010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876068.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP06"/>
    <dbReference type="GeneID" id="76209533"/>
    <dbReference type="OrthoDB" id="382870at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="13542" checksum="83A83CE53FDD1009" modified="2018-07-18" version="1">MFKSDAQNRSKKKQELPKNQMKKALISFVIEQSLIDEGGIKVFEKTMDLLRKKFGCDTSECLNHPDYLIQILNDLPKKAHEKIILAIEEKLKEFEYQEEIRKFTKNLYRIDKVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP21</accession>
    <name>A0A2S2KP21_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_01180</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875992.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP21"/>
    <dbReference type="GeneID" id="76209447"/>
    <dbReference type="OrthoDB" id="4020at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="26"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="79" mass="8859" checksum="FB53C314D1B590B1" modified="2018-07-18" version="1">MSANTIKKAKKLVESGGVVQIEDDLFQIKSSSDPEKSYFVTSDTCECPGFKNFYKFHHGKGLKANCSHLEAIRIFKQDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP31</accession>
    <name>A0A2S2KP31_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SRPBCC family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829020.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP31"/>
    <dbReference type="GeneID" id="76209457"/>
    <dbReference type="OrthoDB" id="10993at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07821">
        <property type="entry name" value="PYR_PYL_RCAR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019587">
        <property type="entry name" value="Polyketide_cyclase/dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39332">
        <property type="entry name" value="BLL4707 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39332:SF7">
        <property type="entry name" value="SRPBCC FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10604">
        <property type="entry name" value="Polyketide_cyc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="17542" checksum="F710E0823A5C8FCE" modified="2018-07-18" version="1">MVKTVKKSFHTGTVKKIITIKASKDKVWRKVSNIVGLPTWVVDVKKTIYLSKNKKGIGAIRLITFADENKIEEHIIAWKDKEYFTYIATEGLPLRAYVATISIKAKSTKLVELTWQSYLNSKKMSETQFLEFLAFMGSFYEASLENLKALLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP53</accession>
    <name>A0A2S2KP53_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polymerase nucleotidyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_02130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP53"/>
    <dbReference type="OrthoDB" id="2367at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="13"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="293" mass="33716" checksum="FB1EF879E9BF1170" modified="2018-07-18" version="1">MTIKNKKLEQFMKELVEQLTAKYPDEIVSFVLFGSATTGEWVRGKSDIDCIVIIKNKKLCKDIEKYLTDLLLVLDAKYDLKLSETCTAYKKTENPTLDLIFKTENKMMFGQPFYVVSQDQLDLKGFKIRQDIKIEIGTRTIASLGLFLQRIKHTGIILYGKDVRKDIPNTVPAIEKVKASFNAMLLLMMSFVIFPFSPKSAFSHAVKANFWACDDVLFALDKPLSTTKQEVCQILSIFGKSKIDSEHLIKSLEYKKTKDNMVMNKGFVIRYMLKSTKFVYGLYMITLQKLFRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP92</accession>
    <name>A0A2S2KP92_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_00790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875956.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP92"/>
    <dbReference type="GeneID" id="76209408"/>
    <dbReference type="OrthoDB" id="5022at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="74" mass="7947" checksum="018429EA004768EE" modified="2018-07-18" version="1">MKSGFAVIIIGIIMFVAGLVMFYSIELGQTNPVLRLVKNIGTFTGLLGMGVTLAGILLNIINKNQPPIQENSEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPA9</accession>
    <name>A0A2S2KPA9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2-phospho-L-lactate guanylyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_02600</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876120.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPA9"/>
    <dbReference type="GeneID" id="76209595"/>
    <dbReference type="OrthoDB" id="11179at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043814">
        <property type="term" value="F:phospholactate guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002835">
        <property type="entry name" value="CofC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03552">
        <property type="entry name" value="F420_cofC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40392">
        <property type="entry name" value="2-PHOSPHO-L-LACTATE GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40392:SF1">
        <property type="entry name" value="2-PHOSPHO-L-LACTATE GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01983">
        <property type="entry name" value="CofC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="212" mass="24467" checksum="C86E4691940D39DE" modified="2018-07-18" version="1">MKIAAIIPVKTFTKAKTRLDLSPEKIDNLCKIMLEEILHTISISPQIDKTIIVTKEEKAIEIGKKFNAVILVDEKEESVNSAVALADKYLLENNYDASIVFPQDIPYIKTQDIDFMLNYKAPPNFAIIIPSRRFDGTNALMRMPIDLMETHYDEDSYKIHMNTAKKHTLNVAMVFVKRIMWDVDNIEDLKFLLEQNEKPQIAEKIKKILEIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPC0</accession>
    <name>A0A2S2KPC0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">LTD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_02700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876128.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPC0"/>
    <dbReference type="GeneID" id="76209605"/>
    <dbReference type="OrthoDB" id="148134at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1260">
        <property type="entry name" value="Lamin Tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001322">
        <property type="entry name" value="Lamin_tail_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036415">
        <property type="entry name" value="Lamin_tail_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00932">
        <property type="entry name" value="LTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74853">
        <property type="entry name" value="Lamin A/C globular tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51841">
        <property type="entry name" value="LTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LTD" evidence="1">
        <location>
            <begin position="20"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="1095" mass="119926" checksum="B15848CF9D8523D8" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPD4</accession>
    <name>A0A2S2KPD4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MarR family transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03070</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146195971.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPD4"/>
    <dbReference type="GeneID" id="76209644"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="12200" checksum="E59AB7DE94CB0ACB" modified="2018-07-18" version="1">MSEEIVEKLSEIQKQNQIIISLLAKLVLKEEEVKEIIINRKTNPKKYIEGYNACDGKHTINEIIKIVGVKQQTLSPILQEWENKGILYSFEKKGLKFYNNYFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPM2</accession>
    <name>A0A2S2KPM2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_03970</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPM2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01610">
        <property type="entry name" value="PAP2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF13">
        <property type="entry name" value="AT30094P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="2">
        <location>
            <begin position="71"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="209" mass="23413" checksum="AFDBE2842CAE37F8" modified="2018-07-18" version="1">MLLTLLFLILTGLVYSGITEIFDQEVVLFFSEHVGDSTLDIIMQYITESGEVFWMLGFGILMLLIPKTRRIGITLMILIVISTLLTGYIKCGVDRDRPDFEYKGAPFPVPISKDTFALFCEGGFDASYPSGHAARSMIFAIILGYALSDRFPRGAYLMFLYPAMISISRIYVLQHYPMDVIGGIVIGVMLAGVMAKRTKLYKIFDKSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPR0</accession>
    <name>A0A2S2KPR0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Roadblock/LAMTOR2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_03120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPR0"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="21477" checksum="033EF1B6A2439720" modified="2018-07-18" version="1">MTIDYNELTKQVLNLYPQVKFAGVVNMKGEIVAGGHKENVEQLLVGDEINMSIHYALQKRDLYTNLAYKIGHETSSITEYEKAAIISVPINSNELFLVRTDNGTDYLKIVDHIHSKLDPQKYIRDEIKAIENEINELKNLKETKSKRKPARKSIVKKKVVKRKPARKTSKSKRKTSKSKRKTIKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPS1</accession>
    <name>A0A2S2KPS1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2070 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_02660</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876124.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPS1"/>
    <dbReference type="GeneID" id="76209601"/>
    <dbReference type="OrthoDB" id="8914at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019204">
        <property type="entry name" value="DUF2070_membrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09843">
        <property type="entry name" value="DUF2070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2070" evidence="2">
        <location>
            <begin position="12"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="582" mass="65577" checksum="96CDCA43BAD39685" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPS2</accession>
    <name>A0A2S2KPS2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chlorite dismutase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_03850</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876230.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPS2"/>
    <dbReference type="GeneID" id="76209727"/>
    <dbReference type="OrthoDB" id="8690at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1030">
        <property type="entry name" value="Apc35880, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010644">
        <property type="entry name" value="ChdC/CLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36843:SF1">
        <property type="entry name" value="COPROHEME DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36843">
        <property type="entry name" value="HEME-DEPENDENT PEROXIDASE YWFI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06778">
        <property type="entry name" value="Chlor_dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="26466" checksum="DF1E37673AB349B9" modified="2018-07-18" version="1">MSEEHSQYYFNFSFFKIDPKWRWMADLAKEESAKEVENVINNSGIMFRSYSNLGLRDDADFLFWFAAKSVEEIQTVIEKIYKTVFGKYILPSRTYLSCTRPSIYVQEQKTHGFVTGNEPKKHVIVYPFTKTREWYLLPKEKRQEIMNEHIEVSKKFPQVILNTTYSFGIHDEDFMLAFEVDNIRDFQDLIMDLRETQVSTYVKNDIPMIVCVKKDIVSLISSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPU5</accession>
    <name>A0A2S2KPU5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucosamine-phosphate N-acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Phosphoglucosamine acetylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Phosphoglucosamine transacetylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_03580</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>D-glucosamine 6-phosphate + acetyl-CoA = N-acetyl-D-glucosamine 6-phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57513"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="2.3.1.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I): step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetyltransferase family. GNA1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.4" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876206.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPU5"/>
    <dbReference type="GeneID" id="76209699"/>
    <dbReference type="OrthoDB" id="43754at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004343">
        <property type="term" value="F:glucosamine 6-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000105">
        <property type="entry name" value="Glucosamine 6-phosphate N-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039143">
        <property type="entry name" value="GNPNAT1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355">
        <property type="entry name" value="GLUCOSAMINE 6-PHOSPHATE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355:SF11">
        <property type="entry name" value="GLUCOSAMINE 6-PHOSPHATE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="9">
        <location>
            <begin position="4"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH33567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="145" mass="16515" checksum="B387975785DB00D8" modified="2018-07-18" version="1">MSQPIIRELRKEDILNGFLTTLDALRKASDIRTDKAMKIFEKINSNPDHIIAVAELDGKIVGTTTLLIEQKFIHDGGVVGHIEDVVVNKDYHGQKIGEKIMKYVLEIAKNRGCYKTILDCTDDVKPFYEKLGFKEIANELRFDHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPV9</accession>
    <name>A0A2S2KPV9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_05110</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876373.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPV9"/>
    <dbReference type="GeneID" id="76209862"/>
    <dbReference type="OrthoDB" id="8553at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002809">
        <property type="entry name" value="EMC3/TMCO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038978">
        <property type="entry name" value="MJ0935"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42198">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42198:SF1">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01956">
        <property type="entry name" value="EMC3_TMCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01415">
        <property type="entry name" value="DUF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="209" mass="23771" checksum="B93111939175E48B" modified="2018-07-18" version="1">MDFNFILLFLDSIHLQFDIFGGQSRQALGSADPIIKGLILSMFAVSGFGILLNLFNSAVRKKMVDQTKLKRIMKETRGWQKERMAAMRAKDQAKIAELGKKSAYMNKMSMEMMQMNMRPMMITFVPLILIFYFVLPQLFSYTVALSPIPLNVIPGDFFQLTCTAEHAAIEGDPCYGHENSLYLWAWYFLSSIAFSGIVMKLTKTSMDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPY4</accession>
    <name>A0A2S2KPY4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10 11">Phosphoenolpyruvate carboxykinase (ATP)</fullName>
            <shortName evidence="10">PCK</shortName>
            <shortName evidence="10">PEP carboxykinase</shortName>
            <shortName evidence="10">PEPCK</shortName>
            <ecNumber evidence="3 10 11">4.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">pckA1</name>
        <name evidence="10" type="synonym">pckA</name>
        <name evidence="12" type="ORF">NZNM25_05230</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>oxaloacetate + ATP = phosphoenolpyruvate + ADP + CO2</text>
            <dbReference type="Rhea" id="RHEA:18617"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.1.1.49"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="10">Binds 1 Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the phosphoenolpyruvate carboxykinase (ATP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.49" evidence="3 10 11"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPY4"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004612">
        <property type="term" value="F:phosphoenolpyruvate carboxykinase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00484">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.8.10:FF:000001">
        <property type="entry name" value="Phosphoenolpyruvate carboxykinase (ATP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.449.10">
        <property type="entry name" value="Phosphoenolpyruvate Carboxykinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.8.10">
        <property type="entry name" value="Phosphoenolpyruvate Carboxykinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00453">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001272">
        <property type="entry name" value="PEP_carboxykinase_ATP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013035">
        <property type="entry name" value="PEP_carboxykinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008210">
        <property type="entry name" value="PEP_carboxykinase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00224">
        <property type="entry name" value="pckA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006820">
        <property type="entry name" value="PRK09344.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006821">
        <property type="entry name" value="PRK09344.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30031:SF0">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE (ATP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30031">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01293">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006294">
        <property type="entry name" value="PEP_crbxkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68923">
        <property type="entry name" value="PEP carboxykinase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53795">
        <property type="entry name" value="PEP carboxykinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="4 10" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="205"/>
            <end position="213"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="binding site" evidence="10">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="408"/>
            <end position="409"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="414"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00453"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="NCBIfam" id="TIGR00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH33732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="499" mass="55397" checksum="9AC9B5A3C96A9EDB" modified="2018-07-18" version="1">MVRIAVEREEGVVNSTGSLSVNTGKYTGRSPDDRFIVFDDKTRDTIDWGKINHQFPSDKFEKLLEKMKKFVDDKELYVFDGFVGADPANRLPIRVINDHVWQSMFSRNLFIRPTKEELENHEPEFTILCINDFVAVPEVDGTRTDVFILIDLTRKIVLIGGTEYAGEMKKSMFGVMNFLLPERGIFPMHCSANIGKKGDTALFFGLSGTGKTTLSADPNRSLIGDDEHGWSDNGTFNFEGGCYAKCINLSKEAEPEIWNAIKPGAVLENVVLKDNVPDYDDNTLTENTRVAYPLDFIPGAVIPSVGGNPKVIVFLTADALGVLPPVSRLTREGAMFHFISGYTSKLAGTERGIKEPKSVFSECFGAPFMPRPASVYAKLLGEKIDKHNTVVYLVNTGWSGGPYGIGKRIKIKYSRAMVTAALSGALDTVEYRHDDLFNLDIPTEVDGVPPEILDSKNTWDDKDLYESSAKKLAEMFIENFKRFENVSPDIIAAGPKATQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPY8</accession>
    <name>A0A2S2KPY8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GtrA family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_03260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPY8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="12979" checksum="C4696B373379F600" modified="2018-07-18" version="1">MLSDYDNYLNTTITIVIGYIIYFGIFSSLFYWNNIERYRGMESNLIKKELFALITSFGLGEIIYLGIRWPTLYYFLELGIEPYIASVISEIIATACYMTSITVFLRKTKTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ38</accession>
    <name>A0A2S2KQ38_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_03590</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876207.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ38"/>
    <dbReference type="GeneID" id="76209700"/>
    <dbReference type="OrthoDB" id="11172at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="146" mass="16007" checksum="95CCA0E69D4CC9FE" modified="2018-07-18" version="1">MGLEKWLAITSVALFAMFAGEMISVYHFMTNVPEEIEFSITFEPDPKIIQFVSIGIAPAGVLAAVAFIMSKHYGSKTVGGLIIAGGIILFAGMTVSYSMLNSIDEKYLTDAVKLIPLLFMVLSAPVIIVGAYLIKHKKLRPKKEYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ45</accession>
    <name>A0A2S2KQ45_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Isopentenyl phosphate kinase</fullName>
            <ecNumber evidence="2">2.7.4.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">NZNM25_06010</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>isopentenyl phosphate + ATP = isopentenyl diphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:33963"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:65078"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.26"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the isopentenyl phosphate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.26" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876451.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ45"/>
    <dbReference type="GeneID" id="76209954"/>
    <dbReference type="OrthoDB" id="15328at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102043">
        <property type="term" value="F:isopentenyl phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024192">
        <property type="entry name" value="Fosfomycin_R_FomA-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040647">
        <property type="entry name" value="IPPK_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654">
        <property type="entry name" value="GLUTAMATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654:SF1">
        <property type="entry name" value="ISOPENTENYL PHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016496">
        <property type="entry name" value="Kin_FomA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/glutamate/uridylate kinase" evidence="12">
        <location>
            <begin position="2"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="5"/>
            <end position="9"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="11">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR016496-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR016496-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH33810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="247" mass="27991" checksum="2040A76BA63FD14B" modified="2018-07-18" version="1">MILIKFGGSIITNKEKPLSPRKKSIDNLSKSLKKIKEPIIIVHGGGSYGHYWSVKYDMHTKEKKYNPRGVAIVKNSMIELNKMILDSLLYFKLNPYCLPPTDFMSGNKPITKKIKEIEKIAKSGLIPVTYGDALWYGQKKTFILSGDKIMTHLAKILKPRLCIFALNEDGLYSDLKSKKLIYELQGELPSISENKMDVTGGMTRKVEEASKISKMGINVFFVNGNKPDRIVKAVKNRKFEGTLFRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ62</accession>
    <name>A0A2S2KQ62_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">MBL fold hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_05940</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876445.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ62"/>
    <dbReference type="GeneID" id="76209946"/>
    <dbReference type="OrthoDB" id="197151at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050313">
        <property type="term" value="F:sulfur dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070813">
        <property type="term" value="P:hydrogen sulfide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07723">
        <property type="entry name" value="hydroxyacylglutathione_hydrolase_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035680">
        <property type="entry name" value="Clx_II_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051682">
        <property type="entry name" value="Mito_Persulfide_Diox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43084">
        <property type="entry name" value="PERSULFIDE DIOXYGENASE ETHE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43084:SF1">
        <property type="entry name" value="PERSULFIDE DIOXYGENASE ETHE1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="6">
        <location>
            <begin position="12"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="202" mass="22703" checksum="FE0DF5D4092E7FF1" modified="2018-07-18" version="1">MKVHQIQVGNMQNFSYIIEDEDTGDAIIIDPSWDLVELEMIIKRNNLKVKYIVNTHHHFDHTLGNEAMAKSTKSPIIQHEFSELPHDISVKDGDFIEFGNSKLKVLHTPGHSKDSICLIGDGKIFSGDTLFVGNCGRIDLPGGSAQDLYHSLFDVLYSLDDNLVLYSGHNYGPSEVSTIGQEKVTNHVMQKRTEQQFIDMMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ66</accession>
    <name>A0A2S2KQ66_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Bifunctional 3-phenylpropionate/cinnamic acid dioxygenase ferredoxin subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hcaC_1</name>
        <name evidence="7" type="ORF">NZNM25_06070</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876457.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ66"/>
    <dbReference type="GeneID" id="76209960"/>
    <dbReference type="OrthoDB" id="6837at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03528">
        <property type="entry name" value="Rieske_RO_ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21496">
        <property type="entry name" value="FERREDOXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21496:SF0">
        <property type="entry name" value="RIESKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="7" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="6">
        <location>
            <begin position="4"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="102" mass="10997" checksum="54C698E3F304EA0E" modified="2018-07-18" version="1">MGKIIAGKTSDIPPGKMIKVSIDGRDILVANIDGEYCATDDSCTHSGSSLSEGKLEEGIITCGWHAAQFDCKTGKLVKFPAKIRDLTSYNVIVESDNIFVEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ77</accession>
    <name>A0A2S2KQ77_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TNase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_05550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829040.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ77"/>
    <dbReference type="GeneID" id="76209905"/>
    <dbReference type="OrthoDB" id="3327at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TNase-like" evidence="1">
        <location>
            <begin position="107"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="222" mass="24074" checksum="CD7C0D174F5FC30A" modified="2018-07-18" version="1">MMNKKIIFGIVLGVVAIAVFGHFASESNQNSATDSDEAAWQLLQKTYLDSECKEKYMGQPDAMQDCFDRVAEEQRLNPLNPELTSTAPPLQLSAKPVSDLDCLGTAQCFIGTVTEIIDGDTIKVDGQSVRFALSSAPEIKGYGGVDARNFIETICPVGSMAIVDEDDGQILGSYGRLVGMVTCNDVNLNSELLDSNLGYMEFRFCDSSEFGGESWAVKHGCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ99</accession>
    <name>A0A2S2KQ99_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5 6">Ornithine carbamoyltransferase</fullName>
            <shortName evidence="5">OTCase</shortName>
            <ecNumber evidence="2 5 6">2.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">argF</name>
        <name evidence="10" type="ORF">NZNM25_05180</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>carbamoyl phosphate + L-ornithine = L-citrulline + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.3" evidence="2 5 6"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876380.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ99"/>
    <dbReference type="GeneID" id="76209869"/>
    <dbReference type="OrthoDB" id="4696at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004585">
        <property type="term" value="F:ornithine carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019240">
        <property type="term" value="P:citrulline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000008">
        <property type="entry name" value="Ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01109">
        <property type="entry name" value="OTCase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002292">
        <property type="entry name" value="Orn/put_carbamltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024904">
        <property type="entry name" value="OTCase_ArgI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00658">
        <property type="entry name" value="orni_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001986">
        <property type="entry name" value="PRK00779.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753">
        <property type="entry name" value="ORNITHINE CARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753:SF3">
        <property type="entry name" value="ORNITHINE TRANSCARBAMYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00102">
        <property type="entry name" value="OTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase carbamoyl-P binding" evidence="9">
        <location>
            <begin position="6"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase Asp/Orn-binding" evidence="8">
        <location>
            <begin position="152"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="55"/>
            <end position="58"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="133"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="227"/>
            <end position="228"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="262"/>
            <end position="263"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="NCBIfam" id="TIGR00658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH33727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="304" mass="33817" checksum="72C786D641E47533" modified="2018-07-18" version="1">MKLKTKNLLTLAELTPKEFLGLIDDSIKLKKELKKGSNKPILKNKTLAMIFQKPSTRTRVSFETGMFQLGGHAINLSSNDMQLSRGESVEDTAKTLSRYVDCIMARVYSHDLIENLSEHSSVPVINGLSDSFHPCQILADFMTIKEKKKKLEGLKIAWIGDGNNVCNSMIYGAALSGIEMSIATPKEFAPDKDVVKKSKKSTNIELTSDPFVATKNSDVVVTDTHSSIHNNDPKRIKKFLPKYQVNSKLMKTAKNDAIFLHCLPAKREQEVTSSVIDGTQSVVWDEAENRLHTQKALLAALIRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQD6</accession>
    <name>A0A2S2KQD6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_05580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876414.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQD6"/>
    <dbReference type="GeneID" id="76209909"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="80" mass="9196" checksum="B94455D4404ABFFD" modified="2018-07-18" version="1">MRVYLGGTKPKRAGYSDNQKDRASTRGLQLLHSQHYQNANKQKTMKSQKEIHEFKKELIKTLRSGDSPYFGLSLESLVNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQE4</accession>
    <name>A0A2S2KQE4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PqqC-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_06250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876474.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQE4"/>
    <dbReference type="GeneID" id="76209981"/>
    <dbReference type="OrthoDB" id="9931at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039068">
        <property type="entry name" value="PqqC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40279:SF3">
        <property type="entry name" value="4-AMINOBENZOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40279">
        <property type="entry name" value="PQQC-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14518">
        <property type="entry name" value="Haem_oxygenas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01236">
        <property type="entry name" value="Haem_oxygenase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="210" mass="23806" checksum="28B07143189ADD16" modified="2018-07-18" version="1">MSIIKKIDEMIEERSLLKHPFYQMWSDGKLTQESLAGYSKEYFQLVKAVPNFMTPIIEKAPNSVTSELIENQQEESDHIKPWIAFAGELGISEDELISYSGLPKTQKAVSILSELMDSFEGGACAMYAFEKEIPKISQTKLDGLAEFYGMTNNDATEYFKLHTEADIRHAASWRNILEKSSTDSNNLIEIADKSISAQNMLLDSCYEEYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQH2</accession>
    <name>A0A2S2KQH2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CheW-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_07130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876579.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQH2"/>
    <dbReference type="GeneID" id="76208962"/>
    <dbReference type="OrthoDB" id="115049at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.180">
        <property type="entry name" value="CheA-289, Domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039315">
        <property type="entry name" value="CheW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036061">
        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002545">
        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22617:SF23">
        <property type="entry name" value="CHEMOTAXIS PROTEIN CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22617">
        <property type="entry name" value="CHEMOTAXIS SENSOR HISTIDINE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01584">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00260">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50341">
        <property type="entry name" value="CheW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
        <property type="entry name" value="CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheW-like" evidence="1">
        <location>
            <begin position="10"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="195" mass="21795" checksum="D26BE9D84D0E3AFB" modified="2018-07-18" version="1">MSTQTVLNETIQVVTFTISEKSSKKGNYAVPIDQVKEIKIVEAITKIPKSKPYVRGVMNLRGKIIPIIDVNKKIGLSNSNTTEIEKQRILVADVNDTLTGLLVDEVNEVLRISTKDVEESPQGAFEENYIKGIAKMNEKLIILVDVSKFLNETKEHIKNDTSMNAKSVEIESEDIVENNNDEIIPEIEAIINQDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQN1</accession>
    <name>A0A2S2KQN1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycosyltransferase RgtA/B/C/D-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_05460</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876558.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQN1"/>
    <dbReference type="GeneID" id="76209897"/>
    <dbReference type="OrthoDB" id="2698at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050297">
        <property type="entry name" value="LipidA_mod_glycosyltrf_83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038731">
        <property type="entry name" value="RgtA/B/C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33908">
        <property type="entry name" value="MANNOSYLTRANSFERASE YKCB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33908:SF11">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13231">
        <property type="entry name" value="PMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="114"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="246"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="278"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="301"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase RgtA/B/C/D-like" evidence="9">
        <location>
            <begin position="92"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH33755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="485" mass="55694" checksum="7F1A0A39BCF6EE8F" modified="2018-07-18" version="1">MNRKYVILIPLILATFIHLINPVGFPDIFFDEGIYMRRAMNMIETGNPQEGYLYDHPYFGQIVLAGILQIANYPPDTSTDPASLQNLYLVPRIFMGIIAVVSTLLVYQIAKEKFGRNAALLSSLLFAVMPYTWIFGRILLDSILLPFLLASILFAIRFKHGGNVWFVPISGIMLGLAIFTKVPVFVFIPLVIWLVYQKRKKFSDMLIWIVPVLLIPLLWPANSIFLDQFDLWVRDVLWQSQRSNSVFEIIGSFLIIDPVLFVIGIAGIIYAAVTKNKFVLFWFVPFVSFLSLIGFKQYFHWIPIIPIMCIAASVWLLEIPKKIKYLQSKIHYVIIGAILIFGFSSTILIITNDLSYNQFEALSYVIENHDGQSTILASPVYTWILYDVFDMDDIPRDYAMILFEPVKTRGITVVADSHFMLDRNRGTELVKAYNDTHSVQYFQGKAKDFDTRIYPYTNMRVNHEAFSIDIRAGDWNIAGNQKNQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQP9</accession>
    <name>A0A2S2KQP9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UBP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876655.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQP9"/>
    <dbReference type="GeneID" id="76208874"/>
    <dbReference type="OrthoDB" id="5776at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBP-type" evidence="1">
        <location>
            <begin position="3"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="84" mass="9738" checksum="1DCACCF9904C9F3C" modified="2018-07-18" version="1">MPKKCEHFLQEKDVSPNTISCEECEKEHLPVVAIRMCLTCGHVGCCDSSMGKHATKHFEETGHPVMKAIPNDIWKWCYIHKEYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQS3</accession>
    <name>A0A2S2KQS3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Roadblock/LAMTOR2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_06980</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876532.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQS3"/>
    <dbReference type="GeneID" id="76210058"/>
    <dbReference type="OrthoDB" id="498at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046600">
        <property type="entry name" value="DUF6659"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20364">
        <property type="entry name" value="DUF6659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="15324" checksum="C8A665812E8B25C3" modified="2018-07-18" version="1">MSDEQFSIEDVKNKIKDILAEPEIRYCGLIDCQGELIAGDFKEGVIPFENDARRRQTFQELAHRVANRKGFDANLGRVKYSSSRREKVVMMSFPFGRYILLVIAEPNVNIDRLAWKIIYKLEHQWSEFHGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQT4</accession>
    <name>A0A2S2KQT4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08070</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQT4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="68" mass="8223" checksum="18549A40770DA844" modified="2018-07-18" version="1">MLCVATREDLRNDILKATEEQQRLMELRKPLLGSKINEDQMNAFRMTTQIMKYEDFIRDTERQLRTMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQV5</accession>
    <name>A0A2S2KQV5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Polyprenyl synthetase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_06260</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866826.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQV5"/>
    <dbReference type="GeneID" id="76209983"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF85">
        <property type="entry name" value="SHORT CHAIN ISOPRENYL DIPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="292" mass="33307" checksum="798037410F6C6FA0" modified="2018-07-18" version="1">MTKFFDQAILDRKNIEINPLLETYGRYIQKIDEALDNELSLYSESEFIEPLKYSLDGGKRIRPIILTLAAESVGKIDENTLSASCAVEFLHMESIIHDDIIDNETMRRQKDPFHIKYGYNTSVLTGDFVLGLILAISSRLDNARITKDLATTAMLMSEGEMIENRLETSEDVTFDDYLKVIEYKTATAFEVAARTGAIIANGTEEQIEALTEYGKNIGIAYQIRDDLLDWKNEDKLFNLLIKKSSDPRDVFNKMEELLKEYSEKARAGLRKIPDNDAKMNLDNLIKFTSFKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQW4</accession>
    <name>A0A2S2KQW4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQW4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="148" mass="17483" checksum="842589342A8B2B76" modified="2018-07-18" version="1">MENMLLENTPDIVVILVPLIISITAIVISIYTAKKSEDVRLYSSLDNTYTQLMKVGVDHPDFRDPHKTNNYKKSFDGSRLYGYESYAFMSINMVATVYDRYKKIPRTWYNIIKIEGDLHKSWFYDNSQKFRDEFVDFIDQKIINSKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQW7</accession>
    <name>A0A2S2KQW7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclophilin TM1367-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876694.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQW7"/>
    <dbReference type="GeneID" id="76208824"/>
    <dbReference type="OrthoDB" id="31132at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025658">
        <property type="entry name" value="Cyclophilin_TM1367"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04126">
        <property type="entry name" value="Cyclophil_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclophilin TM1367-like" evidence="1">
        <location>
            <begin position="5"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="129" mass="14075" checksum="A985BC6E8A7E69F5" modified="2018-07-18" version="1">MKYEVEIKIPPSSSILLELDDVNSPKTVKDLIEKLPFTVDLNVWGDEIYTSKSPISQPEENAKSPVDLNDVAYWPTGKAICLFYGPTPIGSPGEITPASPVNILGKIISPDKSVLNSADGERVTFQLKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQW8</accession>
    <name>A0A2S2KQW8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Universal stress protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_08530</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876700.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQW8"/>
    <dbReference type="GeneID" id="76208818"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="3">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="54"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="155" mass="17435" checksum="1717F88C5FDDD872" modified="2018-07-18" version="1">MYKTILLPHAGTPAGDEALKHAIYVSGKSSRIIILHIVEAVQYPPSFALSSSERNSLLKSIDEVNEEMRQDMEKKMEKYIQQCKENNIKSKVQVVIGDASEIILDTIEKEKVDLIVMSKRRKLKGVKKLLSLGSVSRKIVENTSCPILLIDVENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQX2</accession>
    <name>A0A2S2KQX2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADH-quinone oxidoreductase subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nuoB</name>
        <name evidence="7" type="ORF">NZNM25_06360</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the complex I 20 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQX2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12280:FF:000002">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006138">
        <property type="entry name" value="NADH_UQ_OxRdtase_20Kd_su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01957">
        <property type="entry name" value="nuoB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005012">
        <property type="entry name" value="PRK06411.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11995">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11995:SF14">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56770">
        <property type="entry name" value="HydA/Nqo6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase-like 20kDa subunit" evidence="6">
        <location>
            <begin position="77"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="200" mass="22462" checksum="E19D31F3D256445F" modified="2018-07-18" version="1">MRYINQGEGEYGSFLYKLYLRGYGIRLLKDLVTPENANVFVGKLGDILEKAIDKPLGYAINWGRIWSLWPVHIETACCSVEFGAASSPRYDVERFGIIEAFGSLRQCDLVVVQGTITRKMAPRLRLVYDQMPEPKYVIAMGACAITGGLYFDSYNVLPGIDGVIPVDVYVPGCPPRPETLIQGCMLLQEKIKRMKARKFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR17</accession>
    <name>A0A2S2KR17_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxamine 5'-phosphate oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_06890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876527.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR17"/>
    <dbReference type="GeneID" id="76210050"/>
    <dbReference type="OrthoDB" id="11359at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40660">
        <property type="entry name" value="5'-PHOSPHATE OXIDASE PUTATIVE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40660:SF1">
        <property type="entry name" value="5'-PHOSPHATE OXIDASE PUTATIVE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="PNPOx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyridoxamine 5'-phosphate oxidase N-terminal" evidence="1">
        <location>
            <begin position="4"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="154" mass="17781" checksum="C3E5248C10063DD2" modified="2018-07-18" version="1">MRIPDTIKDFINTQGIFVVGTVSGTTIANVSPRIFFKVEENVIYWLDFFKHKSYKNFQINSWVTVAVFNKDDLEGYQLRGNVRFITDEPIRTEIKESIIKNILQKNSSPKIKSLSEKESTVIQFEPSVCYSLNPEQYSDLSIGSDIDSTFFYNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR33</accession>
    <name>A0A2S2KR33_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ORC1-type DNA replication protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_09040</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in regulation of DNA replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the CDC6/cdc18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876741.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR33"/>
    <dbReference type="GeneID" id="76208766"/>
    <dbReference type="OrthoDB" id="195574at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08768">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01407">
        <property type="entry name" value="ORC1_type_DNA_replic_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015163">
        <property type="entry name" value="Cdc6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055237">
        <property type="entry name" value="Cdc6_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014277">
        <property type="entry name" value="Orc1/Cdc6_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02928">
        <property type="entry name" value="orc1/cdc6 family replication initiation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF26">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22703">
        <property type="entry name" value="Cdc6_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09079">
        <property type="entry name" value="WHD_Cdc6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01074">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="57"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc6 C-terminal" evidence="7">
        <location>
            <begin position="309"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="69"/>
            <end position="73"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="430" mass="49153" checksum="86E1493B7819B77E" modified="2018-07-18" version="1">MSDHELDNIVKKVRKEKKAFSNKAALDTSNPPLEIVSRTDEIKEIVDYIMDYEQGQVVPLVSIYGRSGTGKSTLLKHVCNHLPKVKLCFVNLRKAKTVFGGVNIILNELGQQSLTSAQGMNVAMEKIQETLLNMMDLQKKKLFILALDEFDVLFYDKRGNPSDFVYKLVELIADLKQKGCLAMIFTISNNVLSDYDLDERIRSRIGNSEIFFKPYSKDEMLMILQQRAKVAFDKKINDEILEECAKISFLEHGDARRAVDLLRVSAEIAAKEKKELAVGHVKVALQKIQKDRVEEVIESLSQHSKLICLVMAAKTFFHDKDWHTTMSLYEKYKEYLDAEPVSYRRFSELLKDLENTGLLTSKTSSKGQRGYSSEFQLVMDPQIVGDIIDKDWWNKHVVEPKIALDRLNDAGIKKNSLLTGLTDWNMPYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR56</accession>
    <name>A0A2S2KR56_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">2-isopropylmalate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_09430</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the alpha-IPM synthase/homocitrate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876777.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR56"/>
    <dbReference type="GeneID" id="76208727"/>
    <dbReference type="OrthoDB" id="6555at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046912">
        <property type="term" value="F:acyltransferase activity, acyl groups converted into alkyl on transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054691">
        <property type="entry name" value="LeuA/HCS_post-cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42880">
        <property type="entry name" value="HOMOCITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42880:SF1">
        <property type="entry name" value="ISOPROPYLMALATE_HOMOCITRATE_CITRAMALATE SYNTHASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22617">
        <property type="entry name" value="HCS_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="3">
        <location>
            <begin position="21"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="388" mass="43001" checksum="511ADCE7B02600F8" modified="2018-07-18" version="1">MKDPNHYANLYNAYDKNPKKIRVLDSTLREGEQHPGVSFTNKQRIQIAWMLDYFGVDQIEISPVVSSDHKEATKTIIKQGLKADIVSHGRALKEDIDISLDCDAKWCAAYLGISDIHLKDKLRISREEALRRAVETVEYAKSHGLKIRFTVEDGSRAEPEFLIKVCKAIEEAGVDRISLPDTVGIMRPIGMYNFVKKVKDVVDVPLDAHVHNDIGFAVANAFSACDAGVDQIHTTIDGIGERTGIPPLAEVAVALTYLYKSPNDFRLDMLLDLSRLIEEYTTIKPYDSKPIVGSSAYKHKAGTHLAAILRNPAAYEPIPPRAVGNRRRIVFGELAGKTGAAYLMSLLGLDKNDENAKAVAAGLKGLRMGDLIEIPLEDRLEKKITNDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRD2</accession>
    <name>A0A2S2KRD2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nitroreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_10230</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nitroreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876843.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRD2"/>
    <dbReference type="GeneID" id="89862530"/>
    <dbReference type="OrthoDB" id="287850at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="entry name" value="NADH Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43673">
        <property type="entry name" value="NAD(P)H NITROREDUCTASE YDGI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43673:SF2">
        <property type="entry name" value="NITROREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="FMN-dependent nitroreductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Nitroreductase" evidence="7">
        <location>
            <begin position="161"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Nitroreductase" evidence="7">
        <location>
            <begin position="223"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="322" mass="36625" checksum="D25528A84C43D31C" modified="2018-07-18" version="1">MQFQTIEPSYTSKEGCRLVWKGVDEDDTDVVILNKKELEDLVEIFKKNSTGEVELEDQSSFIRVNSDVTQFTLTNHPLLEAQTTDLQQQVLEYAKVPHEPQYVYIGSKEFYPSVWIRKDDKVQEKFSKTNQKQSLIEAILSSEPEKIKQDLSPTDLFRVFATRRSTRKFDKTKVEDWKVDKILSAADVAPTAGNFQGFQVFLIKNKNTKEALVEAANKQPYVNAPVVLVFCTDPSRVKLKFPPEILEKFSLQDATIAAAFSLLAASGVGLSTIWIGMFDEEKVRKILGTDLRPSSILCIGYPDKKKPPKSRRNLKELIKVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRH1</accession>
    <name>A0A2S2KRH1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ribosomal RNA large subunit methyltransferase N</fullName>
            <ecNumber evidence="12">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">23S rRNA (adenine(2503)-C(2))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">23S rRNA m2A2503 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12 14" type="primary">rlmN</name>
        <name evidence="14" type="ORF">NZNM25_10570</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Specifically methylates position 2 of adenine 2503 in 23S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>adenosine(2503) in 23S rRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2-methyladenosine(2503) in 23S rRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42916"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10152"/>
            <dbReference type="Rhea" id="RHEA-COMP:10282"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74497"/>
            <dbReference type="EC" id="2.1.1.192"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="12">Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the radical SAM superfamily. RlmN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="12"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRH1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070040">
        <property type="term" value="F:rRNA (adenine(2503)-C2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000014">
        <property type="entry name" value="Probable dual-specificity RNA methyltransferase RlmN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01849">
        <property type="entry name" value="RNA_methyltr_RlmN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040072">
        <property type="entry name" value="Methyltransferase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048641">
        <property type="entry name" value="RlmN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027492">
        <property type="entry name" value="RNA_MTrfase_RlmN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004383">
        <property type="entry name" value="rRNA_lsu_MTrfase_RlmN/Cfr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00048">
        <property type="entry name" value="rRNA_mod_RlmN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30544">
        <property type="entry name" value="23S RRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30544:SF5">
        <property type="entry name" value="RADICAL SAM CORE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21016">
        <property type="entry name" value="RlmN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006004">
        <property type="entry name" value="CHP00048"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00275">
        <property type="entry name" value="adenosine_C2_methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01062">
        <property type="entry name" value="methyltransferase_(Class_A)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10 12" id="KW-0408">Iron</keyword>
    <keyword evidence="11 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12 14" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="7 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 12 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Radical SAM core" evidence="13">
        <location>
            <begin position="106"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="active site" description="S-methylcysteine intermediate" evidence="12">
        <location>
            <position position="343"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="170"/>
            <end position="171"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="225"/>
            <end position="227"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GBH34266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="352" mass="38960" checksum="0A24C8AF3B307443" modified="2018-07-18" version="1">MMTDLYRLLPEEMEQLVIDMGYPRYRADQILLPLYYKFPKDINDIPQLPKKLREELTEAGYTIGSAKEIHRIVSSDGETTKLLLNLTDNNSVETVLMQYPSSKIGGHPRSTICVSTQIGCAMGCVFCATGQMGFKTNLTAEHIVSQVIHFAEILEKRGEHVTNLVFMGMGEPMANYDEMIRAIRILTHDRGFGLGQRHITISTIGIVSGIDKLAEENLQIGLAISLHSPNNALRKELVPTAGPNSVKDIIEAGRRYFKKTGRRVTFEYALMDGINDSPEIATELASLLKGNGSHVNLIPINPTAGDFKRPSKQRVLEFERILSNAGVNCTVRVEKGTEISAACGQLRTDIIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRK6</accession>
    <name>A0A2S2KRK6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_10350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRK6"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="159" mass="17679" checksum="2299236F0940FF6B" modified="2018-07-18" version="1">MQDLSMLKSNDKILIIVALVGGMIALAVIIGFIGQGNVRHVTIFWQEKVEHTVVFEDIDGKAQIRGIKGVAGDINPTLLSRTSFAYVLTVINNGTTNHRLYIEGFNVQTDLLKPGQQDTITIYPDKEGEYLYYDKRQDLIPLGKIKIVTVVASDEFQGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRL6</accession>
    <name>A0A2S2KRL6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_10800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876934.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRL6"/>
    <dbReference type="GeneID" id="76208579"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="105" mass="11979" checksum="7E012A4B5D42DA54" modified="2018-07-18" version="1">MATKKRSATRKAKRSVRKAAKTTKRTAAKAKKTVRKAKKTVRKATRTVSKAKRRAQLVANKRKRDLQKANTKVKSMKKALRNAEKTARKQKSTYQKALKRATQTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRT0</accession>
    <name>A0A2S2KRT0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Riboflavin kinase</fullName>
            <ecNumber evidence="5">2.7.1.161</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">CTP-dependent riboflavin kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">CTP:riboflavin 5'-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Flavokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">NZNM25_09490</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>riboflavin + CTP = CDP + FMN + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25021"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.1.161"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the archaeal riboflavin kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.161" evidence="5"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876783.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRT0"/>
    <dbReference type="GeneID" id="76208721"/>
    <dbReference type="OrthoDB" id="30955at2157"/>
    <dbReference type="UniPathway" id="UPA00276">
        <property type="reaction ID" value="UER00929"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008531">
        <property type="term" value="F:riboflavin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009398">
        <property type="term" value="P:FMN biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.30">
        <property type="entry name" value="Riboflavin kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039063">
        <property type="entry name" value="RibK_CTP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023602">
        <property type="entry name" value="Riboflavin_kinase_CTP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023465">
        <property type="entry name" value="Riboflavin_kinase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40706">
        <property type="entry name" value="RIBOFLAVIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40706:SF1">
        <property type="entry name" value="RIBOFLAVIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01982">
        <property type="entry name" value="CTP-dep_RFKase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82114">
        <property type="entry name" value="Riboflavin kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="12 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Riboflavin kinase" evidence="18">
        <location>
            <begin position="102"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00033116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="GBH34158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="227" mass="25427" checksum="8D469F247303C859" modified="2018-07-18" version="1">MTELKIQHILTLSYLLSKGAKHNYVTITTSSLGKNIKKSQQAASKHLVELEQNNFIERIINGRNISVKITPKGFSEMVKLSTILRKSLDSSPSYVELTGTLISGMGEGAYYMGLKGYTTQFKSKIGYVPFPGTLNVRLDQKIHQEAIKQFETLNGVKIKSFSDGKRTYGWVKCFPAKLNNSINCELIILERTHHDDSVIELISKSCLRKTAKLKDGSKISIKIQINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRT8</accession>
    <name>A0A2S2KRT8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11540</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048115112.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRT8"/>
    <dbReference type="GeneID" id="24819644"/>
    <dbReference type="OrthoDB" id="6754at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="128" mass="14722" checksum="5DCD7D6CCC478DD1" modified="2018-07-18" version="1">MPKAGFKSITVSETVYDKFHQVYQNSKDDLTMKGVNSFSGYVTYMLEEMMQKDKTFARYAPKIEKISVDDDRVILKDNIKNRIAEVAVQKGELFCQLCDEKDCVHVGFVFSLPDVYEVLNSRGIKHPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRV1</accession>
    <name>A0A2S2KRV1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">NZNM25_10880</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829071.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRV1"/>
    <dbReference type="GeneID" id="76208571"/>
    <dbReference type="OrthoDB" id="31344at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05145">
        <property type="entry name" value="RIO1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051272">
        <property type="entry name" value="RIO-type_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45723">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RIO kinase" evidence="12">
        <location>
            <begin position="40"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH34297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="270" mass="30850" checksum="B7E433713E901962" modified="2018-07-18" version="1">MNSDDVMSDDLLFDDLSRKIESKVDKKLIFKSKRGGLKDGFKKGKVINEVLDKPTVMTLYKMITDHVIAYVNGAISAGKESVLFWGVDGNDVNVALKIYLVSTSNFKKREPYILGDPRFSHIKKGTKNLVYLWAKKEFRNLSQCYEAGIPVPRPLYLVKNILMMEFIGQDGAPSKLLLYSEVDEDDYHKAICIIKDLYNKAKLVHGDFSEYNIFKTENGLVVFDLGSGVDLRHPNAQEFLKRDINNITKFFHKRGISVEDPIKLFEEIVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRV9</accession>
    <name>A0A2S2KRV9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_09860</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_160049209.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRV9"/>
    <dbReference type="GeneID" id="76208681"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="57" mass="6271" checksum="046A539E2E35F359" modified="2018-07-18" version="1">MDKVQPTWTPDGKTHYALVFVDPKKSAGSWVFSGNALAIHSMNETPFTVSYAVVLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRW5</accession>
    <name>A0A2S2KRW5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">AsnC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877013.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRW5"/>
    <dbReference type="GeneID" id="76208471"/>
    <dbReference type="OrthoDB" id="8136at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF6">
        <property type="entry name" value="REGULATORY PROTEIN ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="1">
        <location>
            <begin position="6"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="79" mass="8693" checksum="2A5916793F81F18B" modified="2018-07-18" version="1">MATAYVLINCELGSEEAVISELKSIEGVKEVHGTFGAYDILAKVESGQVEALRETITWKIRKIPKIRSTLTLMGIEGQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRX6</accession>
    <name>A0A2S2KRX6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Resolvase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).</text>
            <dbReference type="EC" id="3.1.21.10"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRX6"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002732">
        <property type="entry name" value="Hjc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01870">
        <property type="entry name" value="Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="22132" checksum="877E8479002D2ABD" modified="2018-07-18" version="1">MSMRKKVAALTIIKQKTKKEKSKNNQNAARTRRQRGYQWEDAIVKRFNNTEKWKAFRLGSPSIALPDVLAVNTEASAIFTIEAKSGTSTSLPVPGDQIERCLSWIKTFDIYEKRNVLLAFKFLSKKRIDVGKYENRELREFFKIWDESIEITDCVCTYEGKFFAKTNGKRKELFLKECPMPFKTKQRTNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRY2</accession>
    <name>A0A2S2KRY2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_09990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_160049211.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRY2"/>
    <dbReference type="GeneID" id="76208668"/>
    <dbReference type="OrthoDB" id="4814at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="50" mass="5871" checksum="94FB8A421B6B7C69" modified="2018-07-18" version="1">MSQLLEQRQPEPKNILVERTPVKDGTKLSNNSLSWEDIRGLIWLETEDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRZ9</accession>
    <name>A0A2S2KRZ9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11120</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRZ9"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="196" mass="22924" checksum="566310200F70748B" modified="2018-07-18" version="1">MSALSSQSSIPWFDDFVGVAYRYYDLRMNVVPLFTDRKESASIWHDTIHWWVDPSIKLRFVEIGDNYWFIMGSDSQKPDTNLSFFKILPKSENYERFKKGHGGEAYVRLGVYAKKSLDDVKKDALCDCSHEAQDHDENDNDVCLYNNCDCKKFSSFQINLLKRKKTTTDIKFLEENDVKDDPLAWNCLNVNKYSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS22</accession>
    <name>A0A2S2KS22_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_10460</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS22"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="109" mass="12263" checksum="6BA6CA096B6635A0" modified="2018-07-18" version="1">MVIVVAIFSFALDYKINTIFPNAELEREEIKKMSCPEILQKDSSNRYWSSENREIGKAKAAGCSVPEESQSSGLSPYVEFCTPGGFAPVKKIENSTHIFNHDTCIWDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS24</accession>
    <name>A0A2S2KS24_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ArsR family transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS24"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="13499" checksum="E57A5EF3452434F5" modified="2018-07-18" version="1">MKYFFDIYMSRKITLPQLKKYDITQKVIEALADSESRAILFSIIKKGNTAAELSDKLKIPLSSVYKKLGDLEELTLIEVEKWLLSDKGRKYKVYRSRISKADISIRKPEPTLTLVAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSA5</accession>
    <name>A0A2S2KSA5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Circadian clock protein KaiC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_12220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877069.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSA5"/>
    <dbReference type="GeneID" id="76209282"/>
    <dbReference type="OrthoDB" id="27015at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051347">
        <property type="entry name" value="Circadian_clock_KaiC-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010624">
        <property type="entry name" value="KaiC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42926">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42926:SF1">
        <property type="entry name" value="CIRCADIAN CLOCK OSCILLATOR PROTEIN KAIC 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51146">
        <property type="entry name" value="KAIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KaiC" evidence="1">
        <location>
            <begin position="6"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="245" mass="27394" checksum="CB9EAB254AC10953" modified="2018-07-18" version="1">MDATYSKVKTGIPGLDSVISGGMKSGRSVTVSGPAGSGKTTFGLQFLHSGAKDFEEPGVYVTMAQNIREIKDDCKSYGWDFDNLISNDKILMVDARPFKIQEELIGKDDSLYRGEQLPFEHLTKLILSSIKRIEAKRVVIDSVTILSIQYSDKFSMRQGLQAMVQALENFGVTSLILSENSDHSEIPLEWFVTSGIIQLRHTRTGDTMERTIQITKMRGIKHSEQIHPIELDSDGMHLLHPRLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSB5</accession>
    <name>A0A2S2KSB5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Type II secretion system protein GspF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_13340</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877155.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSB5"/>
    <dbReference type="GeneID" id="76209164"/>
    <dbReference type="OrthoDB" id="2769at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.81.30">
        <property type="entry name" value="Type II secretion system (T2SS), domain F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056569">
        <property type="entry name" value="ArlJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042094">
        <property type="entry name" value="T2SS_GspF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402:SF1">
        <property type="entry name" value="TYPE II SECRETION SYSTEM PROTEIN GSPF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Type II secretion system protein GspF" evidence="7">
        <location>
            <begin position="95"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="305" mass="33378" checksum="2FEE456EFF3783C9" modified="2018-07-18" version="1">MESKQKRKIKRRRIAVIEPRKSILKNEILKTASFSIIAATSVISMSFYFSGFSESTTIRDIGLIFGILVGVIPLTIHQLKEVQRRDSIDRNLPVFLLALLSSVQSGANLIKAIEQAGERNLGALTPELRNLRANISWGTPIEDAFENFAERTGTRVARRVTVLLEMAMKIGGDVSENLEMIQKHVSDMQNIEKSRKSALQPYTYTIYISFAVFLAVAVLLTTSFFTEIEKVQDGLLESGSGTEGLFGSLANMDIAQLESGLFNMAIIEAVFGGLAAGKIGSGSYVAGTKHVVVMIIISVIAFNIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSC2</accession>
    <name>A0A2S2KSC2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_12580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_263970073.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSC2"/>
    <dbReference type="GeneID" id="76209244"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="44" mass="4937" checksum="2BF38A2721A8EDDD" modified="2018-07-18" version="1">MKQVGDEFNEGQAGKCPNCKHDIKKHSDKQLAECTLEYLKSGIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSD0</accession>
    <name>A0A2S2KSD0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ammonia monooxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876996.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSD0"/>
    <dbReference type="GeneID" id="76208496"/>
    <dbReference type="OrthoDB" id="7458at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1450.10">
        <property type="entry name" value="Ammonia/particulate methane monooxygenase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024656">
        <property type="entry name" value="AmoA_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037001">
        <property type="entry name" value="NH3/CH4_mOase_suA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12942">
        <property type="entry name" value="Archaeal_AmoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="24303" checksum="0BCA304AD559E605" modified="2018-07-18" version="1">MVWLRRCTHYLFIVVVAVNSTLLTINAGDYIFYTDWAWTSYTVFSISQTLMLIVGATYYLTFTGVPGTATYYALIMTVYTWIAKAAWFSLGYPYDFIVVPVWLPSAMLLDLVYWATKKNKHSLILFGGVLVGMSLPLFNMVNLITVADPLETAFKYPRPTLPPYMTPIEPQVGKFYNSPVALGAGAGAVLACTFAALGCKLNTWTYRWMAAWSKWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSD5</accession>
    <name>A0A2S2KSD5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">pyruvate, phosphate dikinase</fullName>
            <ecNumber evidence="3">2.7.9.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">NZNM25_13540</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.9.1" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877171.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSD5"/>
    <dbReference type="GeneID" id="76209145"/>
    <dbReference type="OrthoDB" id="23397at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050242">
        <property type="term" value="F:pyruvate, phosphate dikinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.10">
        <property type="entry name" value="Phosphohistidine domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.189.10">
        <property type="entry name" value="Pyruvate Phosphate Dikinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008279">
        <property type="entry name" value="PEP-util_enz_mobile_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018274">
        <property type="entry name" value="PEP_util_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000121">
        <property type="entry name" value="PEP_util_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023151">
        <property type="entry name" value="PEP_util_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036637">
        <property type="entry name" value="Phosphohistidine_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002192">
        <property type="entry name" value="PPDK_AMP/ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010121">
        <property type="entry name" value="Pyruvate_phosphate_dikinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004531">
        <property type="entry name" value="PRK05878.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01828">
        <property type="entry name" value="pyru_phos_dikin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22931">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE DIKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22931:SF9">
        <property type="entry name" value="PYRUVATE, PHOSPHATE DIKINASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00391">
        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02896">
        <property type="entry name" value="PEP-utilizers_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01326">
        <property type="entry name" value="PPDK_N"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000853">
        <property type="entry name" value="PPDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52009">
        <property type="entry name" value="Phosphohistidine domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00742">
        <property type="entry name" value="PEP_ENZYMES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00370">
        <property type="entry name" value="PEP_ENZYMES_PHOS_SITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate phosphate dikinase AMP/ATP-binding" evidence="12">
        <location>
            <begin position="16"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate phosphate dikinase AMP/ATP-binding" evidence="12">
        <location>
            <begin position="61"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate phosphate dikinase AMP/ATP-binding" evidence="12">
        <location>
            <begin position="300"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilising enzyme mobile" evidence="11">
        <location>
            <begin position="423"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilising enzyme C-terminal" evidence="13">
        <location>
            <begin position="520"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="751"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="775"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000853-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GBH34563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="889" mass="98847" checksum="F1EB498AC4D5B80C" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSD9</accession>
    <name>A0A2S2KSD9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor Pcc1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CTAG/PCC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877002.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSD9"/>
    <dbReference type="GeneID" id="76208486"/>
    <dbReference type="OrthoDB" id="85338at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015419">
        <property type="entry name" value="CTAG/Pcc1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011470">
        <property type="entry name" value="PRK14887.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09341">
        <property type="entry name" value="Pcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="80" mass="9090" checksum="042C863E7B39D717" modified="2018-07-18" version="1">MSLTCQVKVVLNNISKEKAETIKKALEPDNVNFPEGLSLYVENIDNKLVFNFESKKNMKQLIGTIDEVVEHIQVSLKVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSG9</accession>
    <name>A0A2S2KSG9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Bifunctional 3-phenylpropionate/cinnamic acid dioxygenase ferredoxin subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hcaC_2</name>
        <name evidence="7" type="ORF">NZNM25_13800</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877194.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSG9"/>
    <dbReference type="GeneID" id="76209119"/>
    <dbReference type="OrthoDB" id="6837at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21496">
        <property type="entry name" value="FERREDOXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21496:SF0">
        <property type="entry name" value="RIESKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="7" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="6">
        <location>
            <begin position="3"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="100" mass="11227" checksum="BBDE6209DD1FBD32" modified="2018-07-18" version="1">MTWKKIAEKGEVGAGKGKAFKIEGKQIAVFNQDGYHAIDDLCVHQDGSIAPGKLEGDIVECPLHFWHYNIKTGELTDYLKDVKLETYKVEARDDGIYVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSH1</accession>
    <name>A0A2S2KSH1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_13870</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in DNA damage repair.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nre family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSH1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02096">
        <property type="entry name" value="Nre"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033167">
        <property type="entry name" value="Nre"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006979">
        <property type="entry name" value="Nre_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006978">
        <property type="entry name" value="Nre_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38136">
        <property type="entry name" value="DNA REPAIR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38136:SF2">
        <property type="entry name" value="DNA REPAIR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04895">
        <property type="entry name" value="Nre_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04894">
        <property type="entry name" value="Nre_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Archaeal Nre N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Archaeal Nre C-terminal" evidence="3">
        <location>
            <begin position="265"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="373" mass="41571" checksum="EB84E2A423CA6054" modified="2018-07-18" version="1">MAKWHETLSKHGNLFSSDSISGTSPPSVFVGSYNYPKVFVGPMVPPAHGDTSILDSPERWKGKSLEDIVNFRLNLVRGIQKISVNKTEGRYIENLQEVVMSSQPIDSDLIFEKSTSSHISLDGESAPFGPTGIIHSAKFSGTTSEKSIEKTFYDKDMKAQDAVLNLYNSGIEISKIQKCFSIGMLGQKRKLVPTKWSITATDDIISKSLVDEILDYSIIDSCKIFSYEHLGNVFVIVLFPHRWIYEMIEAWYSNGILGFGSDYEDARGIDHPPAVAGAYFAAKLGVLEYLAKNKIQSGVVILREIRPEYAIPVGVWQVREGVREAMKQKSVLADNFDDAILLASNKMSISKSEWLANGNILKLIHQKTLSDFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSH6</accession>
    <name>A0A2S2KSH6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSH6"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="131" mass="13254" checksum="D23277573762B320" modified="2018-07-18" version="1">MTSMVSIPHVLSGISIVLLVVYGVDVMIGGGGAGEGFLPFDAMTRGIGFGMPPIIFSFIAFFISKKPPFKGLGIMLVITGVLIIIGGIISMASAEESENAARMAGEGGGLIAVGVIIAALGGIKIKKSLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSJ6</accession>
    <name>A0A2S2KSJ6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Mechanosensitive ion channel protein MscS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_13850</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877198.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSJ6"/>
    <dbReference type="GeneID" id="76209114"/>
    <dbReference type="OrthoDB" id="31543at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049278">
        <property type="entry name" value="MS_channel_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045275">
        <property type="entry name" value="MscS_archaea/bacteria_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011066">
        <property type="entry name" value="MscS_channel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221:SF20">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21082">
        <property type="entry name" value="MS_channel_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82689">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="84"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="8">
        <location>
            <begin position="105"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS C-terminal" evidence="9">
        <location>
            <begin position="181"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="274" mass="29957" checksum="E0DFC05EC3D415A5" modified="2018-07-18" version="1">MAFEFLQSLADVELVGGLTLLSLLVGGIIMGVGVIIARTVKLLFMKYYAPKLPQDSAKNFAKLIYFGIILLSFLVFTSNTGLDLSGLLVAGGIFGIVIGFATQSVVSNLISGVFLMIEKPVKQGDNIEIPGSDVSGTLLDISTFSVRVRKFDGTIIRVPNESFFTSNIRSLSSSPVRRTEAVVGIAYKEDIAGAISVLEKQIRKSMPFVLMLPKPEFRIKELADSSVNIEILVWHPRNDWNAVGPHLLKFAKSALDDAGIEIPFPQRVIWQAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSL5</accession>
    <name>A0A2S2KSL5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">yunE</name>
        <name evidence="6" type="ORF">NZNM25_14530</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877256.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSL5"/>
    <dbReference type="GeneID" id="76209042"/>
    <dbReference type="OrthoDB" id="57092at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051598">
        <property type="entry name" value="TSUP/Inactive_protease-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43701">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN MJ0441-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43701:SF2">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN YJNA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="227"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="254" mass="26580" checksum="9A1CD1BC4FC83684" modified="2018-07-18" version="1">MIDQFWLILLGFAAGILGSMIGLGGGIVIVPVLTFLGFPPTTAASNSLFAALSNAVASTISYSKQKRIEISLGLKFGLLSIPGTVLGAMISTDVAPDLFKILFGFVLIASAAYIFLRKQIEPKEKALSKQIMIFAVGASFFAGIISSFFGIGGGIIFVPLMVVGMGMAMKRAAPTSQLILLFASFSGVISHSILGHPDFMQAGFLAAGSFVGGLVGARLSIDIRERYLQIIVSVVVLIAAGKLFLDSLSGTFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSM0</accession>
    <name>A0A2S2KSM0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12390</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877320.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSM0"/>
    <dbReference type="GeneID" id="76209265"/>
    <dbReference type="OrthoDB" id="1479at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="116" mass="13020" checksum="BD6A08004776FCFE" modified="2018-07-18" version="1">MSKSNIIQLSEFDITQKIIESLTNVCTRAVLFSVKNKSKDATQIAEELKISLSTVYKTLSVLEDLALAEVDKYVISPEGKKIKLYRSRIGKVEITLDNLEPSLNLYPNTENPKSNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSM6</accession>
    <name>A0A2S2KSM6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSM6"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="67" mass="7681" checksum="41F5977A35752135" modified="2018-07-18" version="1">MYEYVMSGLDHLLAKSLNEIIEKNLGAKTVKKIDDRLFEKFGLSITQAIEEFDKLDLVLREFFGKGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSP5</accession>
    <name>A0A2S2KSP5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSP5"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="182" mass="21434" checksum="99BA15CC5CADB0EA" modified="2018-07-18" version="1">MWTNKQVWEFEQLNENLRFDVVFAEPKGYETNSWKSKLAFADSEIRDVTVRTFDNLIDGKELWIVNKDQGRLDDQYIPYLMAVMTDIMIEEEICLNFRFENNCLVVSIDSSADVIDCKEFFEEFYHLATGFNYDVDTGIPDDETEAEIYLTRLQEEFDEKMKERLGDKYLPGNNDPDTINAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSQ4</accession>
    <name>A0A2S2KSQ4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_13680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSQ4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Alba-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="80" mass="8647" checksum="BEF3ABCD8ED6AE96" modified="2018-07-18" version="1">MTYVTSAIIQLATMPLITIKARGMTIAHAVDVAQIVLKKTKPAFVIGNIKIGSESLVSQDGRHRDVSSIEISLKRAEASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSR2</accession>
    <name>A0A2S2KSR2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SHOCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12790</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877111.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSR2"/>
    <dbReference type="GeneID" id="76209222"/>
    <dbReference type="OrthoDB" id="2787at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="17768" checksum="A0709BA58AD15CC2" modified="2018-07-18" version="1">MVTENKKSESSDKMIKKKQEKVMDTIAKNYVQTLEHTKNLGKESLGKISEVGLTGTAFLKSKPYWESLKHNSQKIKEKTLEHGAEFKKNSPKFYKKISSGFFYFFESIIGRIKIGTQYGTPSLEILERLAKLKELGILTEEEFTNKKKKILDRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSV3</accession>
    <name>A0A2S2KSV3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Blue (type 1) copper domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_15200</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000008">
        <property type="protein sequence ID" value="GBH34729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSV3"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000923">
        <property type="entry name" value="BlueCu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052721">
        <property type="entry name" value="ET_Amicyanin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507:SF1">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00127">
        <property type="entry name" value="Copper-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Blue (type 1) copper" evidence="3">
        <location>
            <begin position="26"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="274" mass="31786" checksum="938B828FB51506E9" modified="2018-07-18" version="1">MILPHIVLSAYAENSWIISINPYDSQKGQELFKPKELPIFSGDRITWQNNDSTSHRIVSGVPQHPDYSGTFFSTDVISGGENSNSVSLDFIDYAGYYYFCEIHPWYTGKIFFEDRPNILFSTLDISYKILNQNILSIGGLVESDLGNTEYEMLIYDSKNNLIYQKIKLFESDASFNEVIDISDSLWNKDENYLLKLVYGVPSESTSMQIKISKNTTHDKSKYLEFCQDFKLEDSFLFEEIRLPNWFKKTLCWHGNDVTTEKEFVNSLNFFKNSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSX0</accession>
    <name>A0A2S2KSX0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_14520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877255.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSX0"/>
    <dbReference type="GeneID" id="76209043"/>
    <dbReference type="OrthoDB" id="4149at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="150" mass="17497" checksum="09D3F7C961E6DED6" modified="2018-07-18" version="1">MKKFSEFKVGDSFYSTCSISNKELEEYLNFSRVRNAFLEDGQKEKEKIVSGRAILSRMEGEFTRLSQIYGNHIIFVGTDGDPGWENRNTRFLKTLYTDQVLKLKFTISQKDDIDEEYGKIGIDYEGTNQEGETIVISKRNIYRIKKEPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSY6</accession>
    <name>A0A2S2KSY6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_13560</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866896.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSY6"/>
    <dbReference type="GeneID" id="76209143"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02205">
        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051462">
        <property type="entry name" value="CBS_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017158">
        <property type="entry name" value="Tscrpt-reg_CBS-contain_prd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48108">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48108:SF27">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, XRE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037253">
        <property type="entry name" value="HTH_CBS_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="3">
        <location>
            <begin position="14"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="77"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="191" mass="20817" checksum="8AA1656178ABD9E3" modified="2018-07-18" version="1">MIIQIGLLPRIDSIKQIRQKIGITQKKLATMTGVSTSMINQIESGRSQPSYDTAKKIFDSLAKLEGESSSHTAGDFCSKDIVKLKPSNTLHDAIKKMHQLSISQIPVFNNSEVVGVVSEDGIVKHLADVGESELKKAKLADTMDPVPPIVDFDTPANVLIPLIRYSKCILVSKKSKIVGIISASDTLKMME</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSZ9</accession>
    <name>A0A2S2KSZ9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_14920</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866903.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSZ9"/>
    <dbReference type="GeneID" id="76209003"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="187" mass="20854" checksum="AE5FBC013EF27C15" modified="2018-07-18" version="1">MMVKTSIETMECENISHSVTLVPRLGYSAHLLSEIIDFITKKLDDLKQSNCELVLFFDDTDKNHVSAIELERVLTFSSDVLLHIKKRIASVSGLSSIPDILPSSIPMIRTVSAQLFSVIPNCSQRLSELSVHLGSIVLDSAALTKARFDFSQSNNDASIFLDEVKLMVDSKLNKQYPNVDFFKLCDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT01</accession>
    <name>A0A2S2KT01_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acyl-CoA thioesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_15910</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877429.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT01"/>
    <dbReference type="GeneID" id="76208395"/>
    <dbReference type="OrthoDB" id="15030at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052816">
        <property type="term" value="F:long-chain fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03442">
        <property type="entry name" value="BFIT_BACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040170">
        <property type="entry name" value="Cytosol_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11049">
        <property type="entry name" value="ACYL COENZYME A THIOESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11049:SF24">
        <property type="entry name" value="CYTOSOLIC ACYL COENZYME A THIOESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="2">
        <location>
            <begin position="14"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="162" mass="17976" checksum="4DF935B6E4172380" modified="2018-07-18" version="1">MSSKNSQLREKSPSESHAEVIVRMFPSDANPAGNVFGGEILKHIDMVAGIVAQRHSQSNAVTVSMDSVNFLKPVFVGNVLKLNARINYVHNSSMEIEVKAEAEDIVTGIRTLTGTAFVTFVALDKNGKPTHVPKLALKTDEDRTKFEEGKLRMEKRLKKRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT47</accession>
    <name>A0A2S2KT47_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase</fullName>
            <ecNumber evidence="9">1.1.1.302</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">NZNM25_16070</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2,5-diamino-6-(1-D-ribitylamino)pyrimidin-4(3H)-one 5'-phosphate + NAD(+) = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27274"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58890"/>
            <dbReference type="ChEBI" id="CHEBI:59545"/>
            <dbReference type="EC" id="1.1.1.302"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2,5-diamino-6-(1-D-ribitylamino)pyrimidin-4(3H)-one 5'-phosphate + NADP(+) = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27278"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58890"/>
            <dbReference type="ChEBI" id="CHEBI:59545"/>
            <dbReference type="EC" id="1.1.1.302"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; riboflavin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HTP reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.302" evidence="9"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877444.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT47"/>
    <dbReference type="GeneID" id="76208379"/>
    <dbReference type="OrthoDB" id="10178at2157"/>
    <dbReference type="UniPathway" id="UPA00275"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="entry name" value="Dihydrofolate Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006401">
        <property type="entry name" value="Rib_reduct_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011549">
        <property type="entry name" value="RibD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050765">
        <property type="entry name" value="Riboflavin_Biosynth_HTPR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01508">
        <property type="entry name" value="rib_reduct_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00227">
        <property type="entry name" value="ribD_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38011:SF7">
        <property type="entry name" value="2,5-DIAMINO-6-RIBOSYLAMINO-4(3H)-PYRIMIDINONE 5'-PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38011">
        <property type="entry name" value="DIHYDROFOLATE REDUCTASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_8G06820)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="Dihydrofolate reductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0686">Riboflavin biosynthesis</keyword>
    <feature type="domain" description="Bacterial bifunctional deaminase-reductase C-terminal" evidence="10">
        <location>
            <begin position="6"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="NCBIfam" id="TIGR01508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="218" mass="23972" checksum="DCEB1EB091BD1DE6" modified="2018-07-18" version="1">MEKSKPYVILSAATSIDGKIATVTGDSKLSSKLDGIRLHKLRSKVDAILVGKNTVLLDDPMLTVRHTKGKNPIRIVLDSKGSISKNSKIIQTSKKIPTIVVVSNKITKSNLERLKKFPVEIIIAGKNSVDIKLLLKKLSYKKIKSVLVEGGGTTNWEFIKNGLFDELIITLSPFLIGGKDAVSLVEGDGFKKITNSPILRLKSTMRLKNHLVLNYVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT52</accession>
    <name>A0A2S2KT52_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_16410</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877469.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT52"/>
    <dbReference type="GeneID" id="76208344"/>
    <dbReference type="OrthoDB" id="5963at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="68" mass="8000" checksum="4E1E8D2891686032" modified="2018-07-18" version="1">MKDINDIMPKIPNMKWGALMNTPPTNDKVDEMNKIFPSNGKWHTVFEEKDLITIDGKEIRKKDPEKWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT62</accession>
    <name>A0A2S2KT62_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ribosomal RNA small subunit methyltransferase Nep1</fullName>
            <ecNumber evidence="10">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">16S rRNA (pseudouridine-N1-)-methyltransferase Nep1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">nep1</name>
        <name evidence="11" type="ORF">NZNM25_16230</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Methyltransferase involved in ribosomal biogenesis. Specifically catalyzes the N1-methylation of the pseudouridine corresponding to position 914 in M.jannaschii 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a pseudouridine in rRNA + S-adenosyl-L-methionine = an N(1)-methylpseudouridine in rRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46696"/>
            <dbReference type="Rhea" id="RHEA-COMP:11634"/>
            <dbReference type="Rhea" id="RHEA-COMP:13933"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2 10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="10"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877459.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT62"/>
    <dbReference type="GeneID" id="76208362"/>
    <dbReference type="OrthoDB" id="7612at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070037">
        <property type="term" value="F:rRNA (pseudouridine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18088">
        <property type="entry name" value="Nep1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.10:FF:000042">
        <property type="entry name" value="Ribosomal RNA small subunit methyltransferase Nep1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00554">
        <property type="entry name" value="NEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005304">
        <property type="entry name" value="Rbsml_bgen_MeTrfase_EMG1/NEP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023503">
        <property type="entry name" value="Ribosome_NEP1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003207">
        <property type="entry name" value="PRK04171.2-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636">
        <property type="entry name" value="NEP1/MRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636:SF5">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE NEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03587">
        <property type="entry name" value="EMG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 10" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8 10" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="7 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 10 11" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="198"/>
            <end position="203"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="10">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <feature type="site" description="Stabilizes Arg-xx" evidence="10">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="10">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="10">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="222" mass="25413" checksum="F9358FBF27C30F85" modified="2018-07-18" version="1">MISLILSESSLEIVPFELQDHPSIIFHARKLGKHSSEILLDNSWHFAAMKGIKNELKRGRPDLVHFSILEATTIPLYLKNKIKIYVHTLDDKVIYFGQNVHVPKSYHRFEGVIEKLYKEKKITANNETLLELKEKTFSELLDEINPSKVIGFSTRGKPSSYEKIATEISNNDCIIVGGFQKGHFSDFVENKITDLYSIGDESLEGHVVVARLLYECEKTIFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTB1</accession>
    <name>A0A2S2KTB1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">A-type ATP synthase subunit E</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">atpE</name>
        <name evidence="5" type="ORF">NZNM25_16600</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the V-ATPase E subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTB1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.30">
        <property type="entry name" value="ATP synthase, E subunit, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.620">
        <property type="entry name" value="F1F0 ATP synthase subunit B, membrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00311">
        <property type="entry name" value="ATP_synth_E_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038495">
        <property type="entry name" value="ATPase_E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002842">
        <property type="entry name" value="ATPase_V1_Esu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45715">
        <property type="entry name" value="ATPASE H+-TRANSPORTING V1 SUBUNIT E1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01991">
        <property type="entry name" value="vATP-synt_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160527">
        <property type="entry name" value="V-type ATPase subunit E-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="3 4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="199" mass="21702" checksum="F8DBBA3000FEB6BB" modified="2018-07-18" version="1">MTSNSALETTIDKILNNTEKDILSNIKSALVESQQKLDDSIPKLENEYDKIISDGKKEADKIEKQIIGSSDIEARNKQLMLLEEAVAKVFSKALDQIANTDKSGDYSNLIKTLLDESTQVLGTNEITVFTSAKDRDVVQSALSQFSGAELASDTINCLGGIVAKSKDGAMTFDNTIDARIERLKPLIRKDIASKFGVGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTI6</accession>
    <name>A0A2S2KTI6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_17300</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877543.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTI6"/>
    <dbReference type="GeneID" id="76208255"/>
    <dbReference type="OrthoDB" id="8704at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="178" mass="20604" checksum="7FF84AE1870AFC92" modified="2018-07-18" version="1">MGESRISDIKLVNSKDEYSSDENVVINVQFSVEGDLRNAFNEANWTKAYNNNDVSFKMKYGVKLTSGGFRKKELGRTIDTYRKASIFWTRNPKLVNPMKDRRIWVQVAKNFEPFIRLTEEEVRQELFDFDEKFVFKASDLGKGKHKISAEAFASWQKHDYTETGSIKNGSGEIEIIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTJ3</accession>
    <name>A0A2S2KTJ3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Adenosylcobinamide-phosphate guanylyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_15760</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877405.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTJ3"/>
    <dbReference type="GeneID" id="76208411"/>
    <dbReference type="OrthoDB" id="9782at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136:SF86">
        <property type="entry name" value="ADENOSYLCOBINAMIDE-PHOSPHATE GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136">
        <property type="entry name" value="MOLYBDENUM COFACTOR GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MobA-like NTP transferase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="194" mass="21735" checksum="CFAA116BC1E9E43F" modified="2018-07-18" version="1">MIGIVMAGGKGTRMNLDDEKLLLKYKKPIILHVIDSLNNSNLFSKILAITSSNSPKTKKLLQENNIEIFDTPGIGYVEDLNLVLKAIDDQVLVTSGDLPLLDGEIIKKIVNQYDSQKTWTSILVTKKFLSSLGLESDYPVNFSDQVCHYTGISLVNSKKITSLETLDENYIIFDDKRLAFNLNSKQDYDLLSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTK0</accession>
    <name>A0A2S2KTK0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AN1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_17640</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877570.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTK0"/>
    <dbReference type="GeneID" id="76208221"/>
    <dbReference type="OrthoDB" id="26567at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="AN1-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="73" mass="8595" checksum="7801AEA3CD128D25" modified="2018-07-18" version="1">MKSENCAYCGDLTDLPFQCNYCKDPFCSEHRLPEEHRCVKLNQIRAQRFGEKKVIRDGGPNKPNIFKRIFGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTK1</accession>
    <name>A0A2S2KTK1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_16780</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877503.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTK1"/>
    <dbReference type="GeneID" id="76208307"/>
    <dbReference type="OrthoDB" id="12193at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="127" mass="14371" checksum="910C764AE5C04F9E" modified="2018-07-18" version="1">MPGDEIEVPKELREFMLEGAEETFLGQKNGAKKQYRYGNLHIREYDEKFLLHTDKIDPRKDPIGHLVHDAPEVLVGLACAIFGSSQVTKKILNNNSKKKSFASGLLSSIIIGYLGYSATKKIKNYWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTN5</accession>
    <name>A0A2S2KTN5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Blue (type 1) copper domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_17800</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877585.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTN5"/>
    <dbReference type="GeneID" id="76210341"/>
    <dbReference type="OrthoDB" id="11443at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000923">
        <property type="entry name" value="BlueCu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052721">
        <property type="entry name" value="ET_Amicyanin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507:SF1">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00127">
        <property type="entry name" value="Copper-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Blue (type 1) copper" evidence="3">
        <location>
            <begin position="197"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="296" mass="32341" checksum="B0626D1657905BEF" modified="2018-07-18" version="1">MKAAMSLLALLVVSSGYFINESDAEVSQNQPYLLEGSGFAVTEESIKISEIDLGLSSQKQSGSTINFVTEDGFVTLDDEEFTISKLDGKFLREGQYIRINGNVESSRGLDTSISFFGRLVEESKDASVYGFTGRITTSDDSYKVLYTTKLSKLSKITPVSTELKSNALTIHILKGSAGLKTTDNITPGTTEINLRFFSNDRISVQPGTTITIVNDDVVSHSIQSGEENYGSRYDRYTADGRISTGEILPGKSTSITFNDAGFYRLYDPDYQWMGIVAYVFPNSDSLVLGQGKNSGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTP1</accession>
    <name>A0A2S2KTP1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_18030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTP1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="69" mass="7676" checksum="63C7932DDA91F5C3" modified="2018-07-18" version="1">MMKNKKSKAKLIILLGVIWVLITLPLPWIVNNSEVSESQFNTILGIIGIMSIPFIVLGVAWTLKPELTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTR7</accession>
    <name>A0A2S2KTR7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyridine nucleotide-disulfide oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_17620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877568.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTR7"/>
    <dbReference type="GeneID" id="76208223"/>
    <dbReference type="OrthoDB" id="38899at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052541">
        <property type="entry name" value="SQRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755:SF1">
        <property type="entry name" value="FAD-DEPENDENT PYRIDINE NUCLEOTIDE-DISULPHIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="6"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="191"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="382" mass="42236" checksum="99823158A8ABF56E" modified="2018-07-18" version="1">MFNIPHVVILGGGFGGLSAANELRNSLSSSQVQITVIDKKDWFMVGFAKLWIINGTRTFENSIGSLNKLLKKEINFIKDEILKIDLQNKNVKTISHNVSYDYLIISMGAILAPQKIPGLVENGLNLYDYNDLLEIRDRLESMESGKIAISIMGMPYKCPPAPFEASLLIDSMLRKRGIRDSIQIDFYSPAPIALPAAGPEVSKQILELVNYEKIIFHSSCKIKSIESKKLIFENSEADFDLLLAVPPHIAPKVIYDSGLATDPGFISIDRDCKTSFENVFAIGDVTNLTVSETMAVPKAGIFAEGEGITVAKNIISKIQSKEESELFDGKGGCFIESGRNTASIIEVDMFSHSKPSTNLTESTSDNLSKKIEFEKERLSKWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTU5</accession>
    <name>A0A2S2KTU5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fluoride-specific ion channel FluC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">crcB</name>
        <name evidence="8" type="synonym">fluC</name>
        <name evidence="9" type="ORF">NZNM25_17920</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Fluoride-specific ion channel. Important for reducing fluoride concentration in the cell, thus reducing its toxicity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>fluoride(in) = fluoride(out)</text>
            <dbReference type="Rhea" id="RHEA:76159"/>
            <dbReference type="ChEBI" id="CHEBI:17051"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:76160"/>
        </physiologicalReaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="8">Na(+) is not transported, but it plays an essential structural role and its presence is essential for fluoride channel function.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 8">Belongs to the fluoride channel Fluc/FEX (TC 1.A.43) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877596.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTU5"/>
    <dbReference type="GeneID" id="76210329"/>
    <dbReference type="OrthoDB" id="253428at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062054">
        <property type="term" value="F:fluoride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140114">
        <property type="term" value="P:cellular detoxification of fluoride"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00454">
        <property type="entry name" value="FluC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003691">
        <property type="entry name" value="FluC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00494">
        <property type="entry name" value="crcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259">
        <property type="entry name" value="FLUORIDE EXPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259:SF1">
        <property type="entry name" value="FLUORIDE EXPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02537">
        <property type="entry name" value="CRCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <note>structural</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH35001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="126" mass="13470" checksum="8F56256E3E6E8C92" modified="2018-07-18" version="1">MKGLEFVFLAVGSVFGAFLRYKITESPLLFNTLPVNVLIVNVIGAFILGAFVVFSEQWNLDGRYSLFAAVGFCGSLTTMSSFALDSSNLLENNHYGALVINIIANIGLSITALIGGKSLMSAIINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTV5</accession>
    <name>A0A2S2KTV5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3'(2'),5'-bisphosphate nucleotidase CysQ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cysQ</name>
        <name evidence="2" type="ORF">NZNM25_18020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877604.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTV5"/>
    <dbReference type="GeneID" id="76210319"/>
    <dbReference type="OrthoDB" id="58111at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01638">
        <property type="entry name" value="CysQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050725">
        <property type="entry name" value="CysQ/Inositol_MonoPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43028">
        <property type="entry name" value="3'(2'),5'-BISPHOSPHATE NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43028:SF5">
        <property type="entry name" value="3'(2'),5'-BISPHOSPHATE NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="271" mass="30128" checksum="A341535D23C5B306" modified="2018-07-18" version="1">MKDIPIIDKIPELEIAIKAVEDAGKAILEIYQGNFESFTKSDDSPITEADIKSNNIIKEILSQTKYPILSEEDKDDLSRLSKDMIWIVDPLDGTSDFIDKTGEFTVMIGLIKNKKPILGIIGWPTEKTIFVAQKGSGAFRYSNGEWKKITVTKVADISKCRTVGSRHHLSDKEKAFIKKLGIEDFTSIGSSLKVGKISSGEAEAYITTTNKMKEWDSAASYCIISEAGGKMTDMLGNDITYNNKEVNHQNGILVTNGLIHDKIIDEFKKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTZ3</accession>
    <name>A0A2S2KTZ3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DNA starvation/stationary phase protection protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_19040</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the Dps family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877722.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTZ3"/>
    <dbReference type="GeneID" id="76210131"/>
    <dbReference type="OrthoDB" id="8265at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016722">
        <property type="term" value="F:oxidoreductase activity, acting on metal ions"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01043">
        <property type="entry name" value="DPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002177">
        <property type="entry name" value="DPS_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023188">
        <property type="entry name" value="DPS_DNA-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42932:SF3">
        <property type="entry name" value="DNA PROTECTION DURING STARVATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42932">
        <property type="entry name" value="GENERAL STRESS PROTEIN 20U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005900">
        <property type="entry name" value="Dps"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01346">
        <property type="entry name" value="HELNAPAPROT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00818">
        <property type="entry name" value="DPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin/DPS" evidence="3">
        <location>
            <begin position="17"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH35113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="156" mass="17850" checksum="AC2C27A06AC518FD" modified="2018-07-18" version="1">METNIGISSDNAKNIVDILNRLLADEYVLYTKTRNYHWNVVGMQFNDLHKFFESQYGELDGIIDEVAERGRSLGGKTKATLSEFIGNSRIKEQPDTFPEAQTMLRNLLVDHESVIQTLRKDLETCTKLGDAGTSDFLTGLMEQHEKMAWMLRSFLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU06</accession>
    <name>A0A2S2KU06_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NDP-sugar dehydratase or epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_19410</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877752.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU06"/>
    <dbReference type="GeneID" id="76210171"/>
    <dbReference type="OrthoDB" id="4907at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="301" mass="33572" checksum="AE75C37CA5BE18E2" modified="2018-07-18" version="1">MRILVTGGAGFIGKHLVNYLLKKGNKVTIFDNFSNSNKKSIEILQNEDVKIIEGDIREIEDILNAAKDQNIIIHLAAKISVEESIMNPFETFNVNVDGTKNVLEACKKNKISKLIVASSAAVYGDSLSDIKLTENAKINPVSPYGESKARMEKEIIKFLTKNENIDCIILRFFNIFGIGQTSEYAGVITKFLERIKQNQALQVFGDGTQTRDFVSVNDVVSSIYDAIIFGKSGTYNIASGKTITINELAKLMISWSRKNLEIQYLPQKKGDIKFSQAGIGLAKKEIKYSPKFGLEEIKKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU38</accession>
    <name>A0A2S2KU38_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_18920</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866937.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU38"/>
    <dbReference type="GeneID" id="76210119"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050832">
        <property type="entry name" value="Bact_Acetyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43877">
        <property type="entry name" value="AMINOALKYLPHOSPHONATE N-ACETYLTRANSFERASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="4"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH35101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="150" mass="17339" checksum="35323C2A8ED00D20" modified="2018-07-18" version="1">MENVVIRESMAKDIPVILGLLYELGRPRPQKDSEIESFRKLVKQYIDDTDKKILVAEFDGVEIIGMVSIVLLSRLNQTSRELYIPELIVREKFQNQGIGKKLINSCISIAKEKKCHRIRLESGNKRKESHKFYKKLGFEQSGLSFTKNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU51</accession>
    <name>A0A2S2KU51_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_17960</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877599.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU51"/>
    <dbReference type="GeneID" id="76210325"/>
    <dbReference type="OrthoDB" id="2269at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="99" mass="11314" checksum="48818DE98B9E6970" modified="2018-07-18" version="1">MLELDKKVFGNITTKEIIGSDPPEIPNTKDNLEKELTTLLSKLESSSKDNLEKLLKEQKIAEDHINSRPGAMALAQNKIKLFNVYNEKYIQKIKEKLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU83</accession>
    <name>A0A2S2KU83_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Coenzyme Q-binding protein COQ10 START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_19080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU83"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005031">
        <property type="entry name" value="COQ10_START"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03364">
        <property type="entry name" value="Polyketide_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Coenzyme Q-binding protein COQ10 START" evidence="1">
        <location>
            <begin position="75"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="219" mass="24681" checksum="71D0A7FC09F3C546" modified="2018-07-18" version="1">MNVRKSSKPLNPYWQETQSLTLVTKSIDIKTPVENVFTYFARPEHVSDQIKNDTVGMTVVPMDIKEGMGVGTTFRIIGDFSGKRLEWDCETTEFIRNEKIAAKQIEGPFKRWQITNEFKALGNNLTRVTMSVDYEMPFGPLGAILDKAKFAKSAEKGMETALYNVRGLLEGNGSIPVYITLDAYQKLLAEKKKMNDVPVSTALTAIIEKYNEIEAKTQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU97</accession>
    <name>A0A2S2KU97_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GDP-mannose dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_20030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877807.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU97"/>
    <dbReference type="GeneID" id="76210237"/>
    <dbReference type="OrthoDB" id="24929at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028359">
        <property type="entry name" value="UDP_ManNAc/GlcNAc_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43491">
        <property type="entry name" value="UDP-N-ACETYL-D-MANNOSAMINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43491:SF1">
        <property type="entry name" value="UDP-N-ACETYL-D-MANNOSAMINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="245" mass="28066" checksum="8B4EF420F4A2B78D" modified="2018-07-18" version="1">MTDIVLGMGEVGETLFDLLVERNFECVGIDLEESKCKNYSENTVIKNPEYLHVCIPGELTEFVNITVNWVDKIEGLKGVLVHSTVKPGTTKNIQTKTTVPILFSPVRGVHRRFLDDIKKYTKFISSDHKQIDPKIKSDVEKRFEKIDWMSTTKTAELAKILVDTTYYGWLINYAQITKMICDKEGVDFDEMWKFADEIHANLGNRPKMYPGIIGGHCVIPNLSLVEYENLDIVKKVNELFEKSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUA0</accession>
    <name>A0A2S2KUA0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_18190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829113.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUA0"/>
    <dbReference type="GeneID" id="76210302"/>
    <dbReference type="OrthoDB" id="3008at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="50" mass="5972" checksum="ACAB9E6F70088864" modified="2018-07-18" version="1">MIESISEKENYLKDLVLKLLRERNLSNFDMLDSLFAELKQDLENQPKNMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUC5</accession>
    <name>A0A2S2KUC5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Small ribosomal subunit protein eS28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rps28e</name>
        <name evidence="6" type="ORF">NZNM25_18490</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eS28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877674.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUC5"/>
    <dbReference type="GeneID" id="76210074"/>
    <dbReference type="OrthoDB" id="7620at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000145">
        <property type="entry name" value="30S ribosomal protein S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00292">
        <property type="entry name" value="Ribosomal_eS28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000289">
        <property type="entry name" value="Ribosomal_eS28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028626">
        <property type="entry name" value="Ribosomal_eS28_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003080">
        <property type="entry name" value="PRK04007.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01200">
        <property type="entry name" value="Ribosomal_S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00961">
        <property type="entry name" value="RIBOSOMAL_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH35058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="70" mass="7777" checksum="DEBFED489B859D2F" modified="2018-07-18" version="1">MSSTTEEVVQAEIIQIVGRTGIAGEVIQVRVKVLTGKDKGRILTRNVKGSVRVSEILMLRETEREAKKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUF3</accession>
    <name>A0A2S2KUF3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UDP-glucose 4-epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_19780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877787.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUF3"/>
    <dbReference type="GeneID" id="76210208"/>
    <dbReference type="OrthoDB" id="358920at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050177">
        <property type="entry name" value="Lipid_A_modif_metabolic_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245">
        <property type="entry name" value="BIFUNCTIONAL POLYMYXIN RESISTANCE PROTEIN ARNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245:SF13">
        <property type="entry name" value="UDP-D-APIOSE_UDP-D-XYLOSE SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="275" mass="31843" checksum="38B6E6536013B312" modified="2018-07-18" version="1">MTKILVTGSKGFIGKQVVEHLKSSDIISDSIDSKRVDLKNIEEVMKLNKSDIVIHLGGKTIKGLEWNEYFYNNVIGTLNILEYCIKKNIKKMIFVSSYVYGNPKYSPIDEKHPILPHNAYTRSKYLAEQLCEFFAKNSDLNVIILRPFNIFGKTLPKGFLISNLLKSIKTNEKITIVNKNSRRDFLHVDDLTDVILKIKDYDCKFEIFNIGSGKSYSFNEIIEKIEKIKKIKLNVQYEEDKENFIEDIVADISKLKNKIKWNPMISFDEGLQKCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUF9</accession>
    <name>A0A2S2KUF9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VapC9 PIN-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_20220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877836.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUF9"/>
    <dbReference type="GeneID" id="76210256"/>
    <dbReference type="OrthoDB" id="11468at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041120">
        <property type="entry name" value="PIN_9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18477">
        <property type="entry name" value="PIN_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VapC9 PIN-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="122" mass="13573" checksum="C668DC4388A39C02" modified="2018-07-18" version="1">MVEVICDTNFLIHLATKRIKNIDNLDVEIGTISFVVPEVVLAELNKLQKIAEKKQDITMTLNFIKNLKTMPICGSFADKELLDYVKINHSIIGTMDKALKKQIKQAGGSVISLSSDKIVLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUH2</accession>
    <name>A0A2S2KUH2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Chaperone protein DnaJ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">dnaJ</name>
        <name evidence="12" type="ORF">NZNM25_19980</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877803.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUH2"/>
    <dbReference type="GeneID" id="76210231"/>
    <dbReference type="OrthoDB" id="8967at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000005">
        <property type="entry name" value="Chaperone protein dnaJ 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000031">
        <property type="entry name" value="Molecular chaperone DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000002">
        <property type="entry name" value="Molecular chaperone DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02349">
        <property type="entry name" value="DnaJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008035">
        <property type="entry name" value="PRK10767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096:SF10">
        <property type="entry name" value="CHAPERONE PROTEIN DNAJ A6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0346">Stress response</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="10">
        <location>
            <begin position="6"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="11">
        <location>
            <begin position="141"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="9">
        <location>
            <begin position="141"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH35207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="361" mass="39452" checksum="5E99920AD08DB5E8" modified="2018-07-18" version="1">MAAKRDYYEVLGVSKTSSTDEIKKQYRKLALKFHPDRNKSSDAGEHFKEISEAYAVLSDPAKKQLYDQHGHAGVDGRYSSEDIFQGARGDFSDIFGRGGGGFDSIFESIFGRGGGGFGFNQQRGSDILYETSVTLEDVLHGKKMEIDLQKQLQCDNCNGTGCKPGTNKKTCSSCNGQGQIRQTRNMGFASFVTAAPCSSCNGQGSIIEIPCDECKGQGKKKGNKKITFEIPPGIDSGDYTVPNEGNEVPGGSNGDLIVRVRVQPHSKFKRDGKDIFYDQDISMIDAALGCEIVVPTLDGTEKIKVDSGSQPNTIIKLKGKGVTHINSRGKGDQYVRIVVNIPQKLSRHQKKLLEEFQKDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUJ1</accession>
    <name>A0A2S2KUJ1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Translation initiation factor 2 subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">aIF2-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">eIF-2-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">NZNM25_20180</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-2-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877829.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUJ1"/>
    <dbReference type="GeneID" id="76210252"/>
    <dbReference type="OrthoDB" id="84794at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04452">
        <property type="entry name" value="S1_IF2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000015">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.190:FF:000006">
        <property type="entry name" value="Translation initiation factor 2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1130:FF:000002">
        <property type="entry name" value="Translation initiation factor 2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1130">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.190">
        <property type="entry name" value="Translation initiation factor 2, subunit 1, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044126">
        <property type="entry name" value="S1_IF2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024055">
        <property type="entry name" value="TIF2_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024054">
        <property type="entry name" value="TIF2_asu_middle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011488">
        <property type="entry name" value="TIF_2_asu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003064">
        <property type="entry name" value="PRK03987.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07541">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110993">
        <property type="entry name" value="eIF-2-alpha, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116742">
        <property type="entry name" value="eIF2alpha middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="10">
        <location>
            <begin position="12"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH35227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="265" mass="30070" checksum="288B5A20F7E420A4" modified="2018-07-18" version="1">MSTEIQEMPEQGEIVLATVTKVMDHGAYVTLDEYDDIQGFLHISEIAPGWIRSVSRFVKDGEKKVLLVKKVNSKRGDIDLSLKQVSKDQKKQKLKEVKKFEKGKTLLQNVQEKAKLTDEEIEKLEDSIYSRFDSVYDAFIEIGRNGIESVKDLKLAKKTATAIGEICSKIKLPSVEIRGIMEITSDKSDGVEIIKKILLDAIKKDPTIDITYLGAPKYRLSITAENFKSAEKSLKPIIEEIQDNIEKKKGAFKFTREESKKTREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNR6</accession>
    <name>A0A2S2KNR6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00910</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875967.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNR6"/>
    <dbReference type="GeneID" id="76209419"/>
    <dbReference type="OrthoDB" id="7195at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="96" mass="10694" checksum="A5809C4D671169F9" modified="2018-07-18" version="1">MGDGIGGAVIGILTNNAFELLVSHTRKDNQEEYGKTEKIMIAKVDDPEQPQYGETTKEDLERALKGKFVSCNVQYRDSKTDVLVCNVFVQKPPEGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNS2</accession>
    <name>A0A2S2KNS2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00670</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_263970043.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNS2"/>
    <dbReference type="GeneID" id="76209396"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="43" mass="5170" checksum="6190159117931C3C" modified="2018-07-18" version="1">MIQDKNNQRAIAEYLNRIHERRAKLEKSNEIGFNFIEIGDKHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNT4</accession>
    <name>A0A2S2KNT4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pentapeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00870</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875963.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNT4"/>
    <dbReference type="GeneID" id="76209415"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="372" mass="42998" checksum="67055C1B6F2BF29A" modified="2018-07-18" version="1">MINFNNDVDFSHAHFHGIQTSITHVQFNGTKTNFSHAKFDTESVSFLMTHFNGTNVDFSYTKFHGSEASFNSTKFHAQKINFAHSVFLSDKILFYEASFMGEINFGHVQFNNFTSFGDAIIDKNAEIEFNSDLSKVSFYNVKIGTNMKFGLYTKWNKQNKIYDEIRLEENDSNGLRLIAGIISQYQGLRDNYEYNFNYEDAGKFFIRESEVLRNYEDPNPFENKTTFSNKLKSAFSKSKKIEEKKSEEEILKIKKKSRFHRYVSIPFVYKHIADYGESYKKPLGILIIIFTYTMLSSAVLQHVENNLDILDAFAYGTSRALGAFVPYLDVADTTDKPWDYAVKAIALPLTGLSFIALKRRFERKYRNTVLGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP02</accession>
    <name>A0A2S2KP02_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829014.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP02"/>
    <dbReference type="GeneID" id="76209331"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="49" mass="6074" checksum="17949E557192ADB8" modified="2018-07-18" version="1">MMIRIEDKRHKELLKQKEELEKNRPHDITAMRGWKHSMSKILQELELFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP65</accession>
    <name>A0A2S2KP65_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carbon-nitrogen hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_02370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876099.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP65"/>
    <dbReference type="GeneID" id="76209571"/>
    <dbReference type="OrthoDB" id="41015at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07197">
        <property type="entry name" value="nitrilase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001110">
        <property type="entry name" value="UPF0012_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088:SF27">
        <property type="entry name" value="DEAMINATED GLUTATHIONE AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088">
        <property type="entry name" value="NITRILASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01227">
        <property type="entry name" value="UPF0012"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="244" mass="27788" checksum="089C37B595FA2A51" modified="2018-07-18" version="1">MVKLGLIQTTSYESNQKGIAQVSKTIKKLGQKETKIVCLPEQWLKNNIIKDFDSEFLEFKKIARDFSMTIIPGAFYEIRKNTSIIAPVIGPEGEFIGRQEKIHPFDYERDSVKPGNEAKIFKTSCKFGIIICYDMVFPNVANVLAKKGAQVLFSPSRIVRRGIAPWEMYVQVRALENRIPILAANVENQRFGGNSIIVDLTEDNKVVTTKIIKLKGASEKSKEFILEKYEKSRKSRFADSNKFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP89</accession>
    <name>A0A2S2KP89_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutamine synthetase</fullName>
            <ecNumber evidence="3">6.3.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glutamate--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">NZNM25_02940</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-glutamate + NH4(+) + ATP = L-glutamine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15 16">Belongs to the glutamine synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876149.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP89"/>
    <dbReference type="GeneID" id="76209631"/>
    <dbReference type="OrthoDB" id="36124at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006576">
        <property type="term" value="P:biogenic amine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019740">
        <property type="term" value="P:nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000005">
        <property type="entry name" value="Probable glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004809">
        <property type="entry name" value="Gln_synth_I"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00653">
        <property type="entry name" value="GlnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43407">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43407:SF1">
        <property type="entry name" value="LENGSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51986">
        <property type="entry name" value="GS_BETA_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 19" id="KW-0436">Ligase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS beta-grasp" evidence="17">
        <location>
            <begin position="13"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="GS catalytic" evidence="18">
        <location>
            <begin position="112"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="273"/>
            <end position="274"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="280"/>
            <end position="282"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="O-AMP-tyrosine" evidence="14">
        <location>
            <position position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="GBH33503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="481" mass="54007" checksum="0B5B0903E5679D41" modified="2018-07-18" version="1">MNADQVLQTIQDEKISFIDFWFVDIFGELHNVGMPSYAIDKDSFVNGLEKLDASSIVGFKSVNHSDMILLPDPSSFKILPNDYDPGNRKNARIFCDLYDGNSNKESRYNRDSRGIANKASEKLSDFGLTHTNWGPEIEFFVFDTINVYPSPYAATHSYGGSGYSIESKESPWAKGNVSTAIDLKEGYYPSQPKDTLEGFRKDICDDLYNYFGIKIEAEHHEVATSGQCEINLVYDEMIAMADNVIAVKNLVKVKAKRKNKVATFMPKPIFGDNASAMHTHQSLWNGKTNVMYDKDDEEAQMSQTGRYYIGGILNHASALCAISNPTTNSYKRLVPGFEAPVNVCWGLGNRSAAIRVPMYNRNQEKSKRLEYRVPDPTANIYLLEAALLLAGLDGIKNKIDPGNPIEENVYKLSAEKKREYKIGTLPVSLKDALDSLNSDSNFLEDVFTKDFLDAYSEIKYKEYTAFAQTPTAWEVSMYADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPC9</accession>
    <name>A0A2S2KPC9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methionine--tRNA ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_02800</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPC9"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02800">
        <property type="entry name" value="tRNA_bind_EcMetRS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004495">
        <property type="entry name" value="Met-tRNA-synth_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051270">
        <property type="entry name" value="Tyrosine-tRNA_ligase_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586">
        <property type="entry name" value="TRNA-AMINOACYLATION COFACTOR ARC1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586:SF37">
        <property type="entry name" value="TRNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="3">
        <location>
            <begin position="11"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="112" mass="12195" checksum="F730509CE04C7CCE" modified="2018-07-18" version="1">MSDMSNVSYDDFAKLDIRVAKILATEPIEGKSKIIKGLIDLGNDDKRDVIIGGAQYYQPEDLVGKTVIVIANLEPKKMAGVESNAMLLAADVDDKPFWLTVNEDVPLGSSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPD5</accession>
    <name>A0A2S2KPD5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA polymerase IV</fullName>
            <shortName evidence="15">Pol IV</shortName>
            <ecNumber evidence="15">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">dbh</name>
        <name evidence="18" type="ORF">NZNM25_02450</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 20">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the DNA polymerase type-Y family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="15"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876107.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPD5"/>
    <dbReference type="GeneID" id="76209579"/>
    <dbReference type="OrthoDB" id="372207at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03586">
        <property type="entry name" value="PolY_Pol_IV_kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.60:FF:000009">
        <property type="entry name" value="DNA polymerase IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.100">
        <property type="entry name" value="DNA polymerase, Y-family, little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01113">
        <property type="entry name" value="DNApol_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036775">
        <property type="entry name" value="DNA_pol_Y-fam_lit_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017961">
        <property type="entry name" value="DNA_pol_Y-fam_little_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050116">
        <property type="entry name" value="DNA_polymerase-Y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022880">
        <property type="entry name" value="DNApol_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053848">
        <property type="entry name" value="IMS_HHH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002677">
        <property type="entry name" value="PRK02406.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11076:SF33">
        <property type="entry name" value="DNA POLYMERASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11076">
        <property type="entry name" value="DNA REPAIR POLYMERASE UMUC / TRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11799">
        <property type="entry name" value="IMS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21999">
        <property type="entry name" value="IMS_HHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100879">
        <property type="entry name" value="Lesion bypass DNA polymerase (Y-family), little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 15" id="KW-0235">DNA replication</keyword>
    <keyword evidence="12 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 15" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="10 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 15" id="KW-0515">Mutator protein</keyword>
    <keyword evidence="6 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="UmuC" evidence="17">
        <location>
            <begin position="6"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="312"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Substrate discrimination" evidence="15">
        <location>
            <position position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="GBH33454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="364" mass="41424" checksum="3F1983713BE5C02E" modified="2018-07-18" version="1">METRVVFHIDFDYFYAQCEETRSPELKTKPVCVCVFSDRGGDSGAIATANYAARKYGVKSGMPISFAKKRLEGRNDAVFLPVDFDFYSEISEKAMKIMKFNADIFEYVGRDEAYLDVTKRVEGDFKKASHLAQQIKNSIREKIKLSCSIGISSNKLISKIASDYRKPDGLTVVTPDNMNEFLNPLKIRVIPGIGKKTEERFSEMGLETIQDLKKLDVFTLNKEFGRKNGAYIYNAVRGTYNEPVKEREASIQYSKISTLKKDSKDYQFLSENIIDLCKEVHGILLKNNRMFKSVGIHLIQSDLSSKSRSRMLKNPTSSLEELQKNADQLLREALENQTITIRRLGVKVTELSEVQGQSSITNYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPE4</accession>
    <name>A0A2S2KPE4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_02900</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876145.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPE4"/>
    <dbReference type="GeneID" id="76209627"/>
    <dbReference type="OrthoDB" id="10960at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3430.10:FF:000011">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029:SF21">
        <property type="entry name" value="AMMONIUM TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="356"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="7">
        <location>
            <begin position="9"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="435" mass="45990" checksum="A851EF9D3DDDA61D" modified="2018-07-18" version="1">MVLDSGDTAWMLVAGSLVLLMIPALGLFESGLLRKKNAASIFMQIFFGLALLSVMWFVFGFSLSFGDSTMGLVGNMDWVFLKGVPSDGPLEQYAPTIPGVLFVKFQLMFAAITPLLLTGTIAERMKFSSFIIFIAAWSMLIYYPLVHWVWGGGWLSQLGVVDFAGGIVIHTSVGMAALAAAIVLGKRRHYGPAIMIPHSIPLAVLGSSLLWLGWFGFNAGSALSASGGVAGNTVIVTHMASSVSALIWAGLSWVRTGKPSVVATINGAIAGLAGITPASGFVSAEHAFVIGIAIGVISYSGVVLFKEKLKIDDALDVSSVHGVAGIVGALSIGIFASTVINPGGVDGLLFGNPDQLWIQAVGVGVAAAMGFGGTWIILQVMKHLIGIRVSPEVEDVGLDISEHAESAYSDEEEFMLDMDTFTEELQEKDEIFRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPF4</accession>
    <name>A0A2S2KPF4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Inositol-3-phosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_02380</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the myo-inositol 1-phosphate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876100.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPF4"/>
    <dbReference type="GeneID" id="76209572"/>
    <dbReference type="OrthoDB" id="80661at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004512">
        <property type="term" value="F:inositol-3-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052199">
        <property type="entry name" value="MIPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002587">
        <property type="entry name" value="Myo-inos-1-P_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013021">
        <property type="entry name" value="Myo-inos-1-P_Synthase_GAPDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43125">
        <property type="entry name" value="INOSITOL-3-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43125:SF1">
        <property type="entry name" value="INOSITOL-3-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01658">
        <property type="entry name" value="Inos-1-P_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015578">
        <property type="entry name" value="Myoinos-ppht_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myo-inositol-1-phosphate synthase GAPDH-like" evidence="2">
        <location>
            <begin position="199"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="364" mass="40553" checksum="BF64B7FADB22D6F4" modified="2018-07-18" version="1">MADRIKVGLVGIGNCFAGLIQGIEYYRRNPSQEVTGIIHDKLAGYGIHDIDFVCGFDVGENKVGKLLNEAIYEYPNMVDWIPRDEMPKTDAKVHESPILDGVGLWVENRIKPIQSDKTPEQIADEVKKIIKDTGVEIIVSYLPVGSDKVTEFWAQICLDTGTAFVNCIPSFIASDEKWAKKFEEKNIPCIGDDIKGQVGATIVHRTLAKLCSDRGTKIEKTYQINVGGNTDFLNMKEQDRLASKRISKTESVQSQLKDRLDDDQIYVGPSDFIPFLGNTKLMFMRIEGRQWANIPYNMEVRLEVDDKANSAGIVIDAIRLARIALDRGVGGPIKPASAYLMKHPIEQTSDVKAKAECEKFVAGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPH0</accession>
    <name>A0A2S2KPH0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase</fullName>
            <ecNumber evidence="3">4.3.1.32</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_02850</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil + S-adenosyl-L-methionine = 7,8-didemethyl-8-hydroxy-5-deazariboflavin + 5'-deoxyadenosine + L-methionine + NH4(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55204"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:59904"/>
            <dbReference type="ChEBI" id="CHEBI:85936"/>
            <dbReference type="EC" id="4.3.1.32"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; coenzyme F0 biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.32" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876140.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPH0"/>
    <dbReference type="GeneID" id="76209622"/>
    <dbReference type="OrthoDB" id="35347at2157"/>
    <dbReference type="UniPathway" id="UPA00072"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044689">
        <property type="term" value="F:7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01611">
        <property type="entry name" value="FO_synth_sub1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019939">
        <property type="entry name" value="CofG_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034405">
        <property type="entry name" value="F420"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03550">
        <property type="entry name" value="F420_cofG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004884">
        <property type="entry name" value="PRK06245.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076:SF15">
        <property type="entry name" value="7,8-DIDEMETHYL-8-HYDROXY-5-DEAZARIBOFLAVIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076">
        <property type="entry name" value="FO SYNTHASE (COFH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00294">
        <property type="entry name" value="7_8-didemethyl-8-hydroxy-5-dea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01388">
        <property type="entry name" value="7_8-didemethyl-8-hydroxy-5-dea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01064">
        <property type="entry name" value="F420__menaquinone_cofactor_bio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="11">
        <location>
            <begin position="55"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH33494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="389" mass="44580" checksum="C52B8AF863A6D7BE" modified="2018-07-18" version="1">MNKLVLNSESLNKVLENKTISREDIFEIYQNSLKDPTELFSVSQNLREKFKKNTVTFSKKAFFNIINLCKDTCSYCTYKAEPGEEKLSLMSKQQILELLQLAKKYRCVETLFVTGEQPEQRYQEAREWLKENGFKSTVEYLIHSSELALEIGLFPHTNAGNLNYEEMKELKKTNVSMGVMLENVSERLTERGMPHYLAASKKPKARLQVLENSGKIGIPITTGILVGIGETIGEVIDSILAIRELHSKYGNIQEVILQNFQPKPDTVMKNKPSADERYFKTIVALSRIIMPEMNIQIPPNLSPKSYQSFLSAGINDWGGISPLTPDFVNPEFAWPEINKVSKESKDAGFDLKCRFPVYPEFFSFISKELRDKMAVIENEEGLVKEEYWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPH2</accession>
    <name>A0A2S2KPH2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876172.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPH2"/>
    <dbReference type="GeneID" id="76209658"/>
    <dbReference type="OrthoDB" id="1889at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="110" mass="12166" checksum="57E2150E85A4ED43" modified="2018-07-18" version="1">MTVIVTRKPGGITQLFNTETGRVTYKCKAECAYMLIEAFGGIVQFNQKGAMATVTGHITSLESCDVLKKGHPNYHDALNSIISAHKEFAQNISDSHQKEIKELENKLVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPI6</accession>
    <name>A0A2S2KPI6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876159.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPI6"/>
    <dbReference type="GeneID" id="76209642"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="67" mass="7899" checksum="3A8B9A8F75B767C1" modified="2018-07-18" version="1">MAKKEKSEKLLEEINKKLDIMIEIQILEKSEDDQLKILKRLGYSSKEASEFTGISYSSIRHRKGWKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPL6</accession>
    <name>A0A2S2KPL6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF167 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_03840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0235 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876229.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPL6"/>
    <dbReference type="GeneID" id="76209726"/>
    <dbReference type="OrthoDB" id="10336at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1200.10">
        <property type="entry name" value="YggU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003746">
        <property type="entry name" value="DUF167"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036591">
        <property type="entry name" value="YggU-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00251">
        <property type="entry name" value="DUF167 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02594">
        <property type="entry name" value="DUF167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01152">
        <property type="entry name" value="DUF167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69786">
        <property type="entry name" value="YggU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="70" mass="8004" checksum="0853D48859581438" modified="2018-07-18" version="1">MIYKVHVTFSKEFLEINENQIKIGIKSKPLKGEANKEIIKKIAKHFGISTSLVQIKSGHKSQEKIIEILQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPT1</accession>
    <name>A0A2S2KPT1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04810</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPT1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="122" mass="13326" checksum="6756D2A151F685E5" modified="2018-07-18" version="1">MTLKLKLHGKATLFMKKEFVVKSIDSAPDGAPYVVVSLSSIKDLKESAQNQQSPFGAPKVMGFTNMNDMMKDLNKMMSGMGGGMGMQGVTQLKLEMHEYKDMGLSVGDKVFLELSKEESLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPU3</accession>
    <name>A0A2S2KPU3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04770</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876346.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPU3"/>
    <dbReference type="GeneID" id="76209829"/>
    <dbReference type="OrthoDB" id="1779at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="69" mass="8211" checksum="9500EBC2EDA204F6" modified="2018-07-18" version="1">MADCVQTWRRQLRIQELANIARDKLESGTEITQVYEILDEIMVSKWRSIPTTRKQYLNSVKKVLENQNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPU6</accession>
    <name>A0A2S2KPU6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TATA-box-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Box A-binding protein</fullName>
            <shortName evidence="7">BAP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">TATA sequence-binding protein</fullName>
            <shortName evidence="7">TBP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">TATA-box factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tbp</name>
        <name evidence="9" type="ORF">NZNM25_02790</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7 8">General factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Binds specifically to the TATA box promoter element which lies close to the position of transcription initiation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the TBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876135.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPU6"/>
    <dbReference type="GeneID" id="56067307"/>
    <dbReference type="OrthoDB" id="350539at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000007">
        <property type="entry name" value="TATA-box-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000010">
        <property type="entry name" value="TATA-box-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00408">
        <property type="entry name" value="TATA_bind_prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030491">
        <property type="entry name" value="TBP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00686">
        <property type="entry name" value="TIFACTORIID"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00351">
        <property type="entry name" value="TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="repeat" description="1" evidence="7">
        <location>
            <begin position="10"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="repeat" description="2" evidence="7">
        <location>
            <begin position="101"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="186" mass="20694" checksum="FCE342993C784681" modified="2018-07-18" version="1">MPQTKPIVSVENVVASASVDQKMDLNEITKTFPDVEYHPDQFPGLVFRLKTPKTATLIFTSGKMVCTGSKSEEMARKAVKTVVQKLRKGGIKVKKDATVQIQNIVASINLGGKIHLEQAARTLPRSMYEPEQFPGLIHRMLDPKTVILLFSSGKLVCTGAKQEPDVYRSVNNLHALLEEKNLMIYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPU7</accession>
    <name>A0A2S2KPU7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_04830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPU7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="122" mass="14359" checksum="6FF90D0D138C180B" modified="2018-07-18" version="1">MVNLDFKKSAIKHPTINDDGLMQSDLLHVFFDVDTGCDYPDGDEWFIVELLFPHDVKIPDNLKGVDYFTTISVDEGKTFWHHRELIRFKYGKSKKLDDALEFLESKYKELHELLEPLQKDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPV7</accession>
    <name>A0A2S2KPV7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl13</name>
        <name evidence="5" type="ORF">NZNM25_04700</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876339.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPV7"/>
    <dbReference type="GeneID" id="76209822"/>
    <dbReference type="OrthoDB" id="7668at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_uL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_uL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005823">
        <property type="entry name" value="Ribosomal_uL13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005755">
        <property type="entry name" value="Ribosomal_uL13_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_uL13_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01077">
        <property type="entry name" value="L13_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002181">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="152" mass="17475" checksum="334FDCBB02B4638E" modified="2018-07-18" version="1">MAEQVIRTDRPIVVDGTNHIAGRLSSYVAKLLIKGNRVSIVNCEKIMMSGTRVNQIKEYREFLEINSVINPKHGPVHYRRPDTLIAKMIRQMLPFDRKPSGKESYQRLRTYIGSPKEIKSLEKIQFEKAKIKKTAANYTSVGELCRIIGWTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPX1</accession>
    <name>A0A2S2KPX1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_05070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPX1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="49" mass="5169" checksum="D4ECA5C0BA4BD030" modified="2018-07-18" version="1">MKTPQNSNWDSMVHAPFKKVVKYDLICMGIGVVIGMGAGAYLVASGLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPY5</accession>
    <name>A0A2S2KPY5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04550</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876326.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPY5"/>
    <dbReference type="GeneID" id="76209807"/>
    <dbReference type="OrthoDB" id="8651at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="103" mass="11931" checksum="CA11EEF17483B22C" modified="2018-07-18" version="1">MLLDLPVLEKGNFYFIKDGDSQFILEDKTKRGLTIKETSVDEKLNVTADKGMIHDMDGIGHWVVIRWYFSKDSYDLSQVLEHAQAMEKKYTELRELTCPDDDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ56</accession>
    <name>A0A2S2KQ56_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Magnesium transport protein CorA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">corA</name>
        <name evidence="9" type="ORF">NZNM25_06110</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876461.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ56"/>
    <dbReference type="GeneID" id="76209965"/>
    <dbReference type="OrthoDB" id="28779at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015087">
        <property type="term" value="F:cobalt ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12822">
        <property type="entry name" value="TmCorA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.20">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045861">
        <property type="entry name" value="CorA_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46494">
        <property type="entry name" value="CORA FAMILY METAL ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46494:SF1">
        <property type="entry name" value="CORA FAMILY METAL ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143865">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="341" mass="39264" checksum="E649B8BFE784794B" modified="2018-07-18" version="1">MKKGFITKKLRTGKRILEEDSSQRKIERIQGGKFTWLDLQNPDRGQMEKLAQEHNLNSLNVEDCLTKFELQKLDSYDNHFFVILHFPPLSQKAGISKNSQLSIFIGKDFLITVHQGDLNPLVELVELCMGDSDQERKNRLLEKSPGHLLHEIIDVLVDDLLHTSRKIIANLDEMEDRVFDERKSVARSISLLRREINRLRRIANPLKKFVLEIAKNIERYSDKEHEELTLYFDDVIDHIDKVIETLEESRETMEIYKDTDFVLSTEKTNKVLGVLTIIFTLAIPATVIGTFYGMNVNLPGGIGDDVMFLGPFTTFIIVILASAIPAILMFVYFRRLGWISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ60</accession>
    <name>A0A2S2KQ60_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">OsmC family peroxiredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_05350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876396.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ60"/>
    <dbReference type="GeneID" id="76209886"/>
    <dbReference type="OrthoDB" id="133354at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003718">
        <property type="entry name" value="OsmC/Ohr_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052924">
        <property type="entry name" value="OsmC/Ohr_hydroprdx_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036102">
        <property type="entry name" value="OsmC/Ohrsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35368">
        <property type="entry name" value="HYDROPEROXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35368:SF1">
        <property type="entry name" value="HYDROPEROXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02566">
        <property type="entry name" value="OsmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82784">
        <property type="entry name" value="OsmC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="175" mass="19432" checksum="D2F5A9EAB539DF76" modified="2018-07-18" version="1">MTKINNVDLDKVANTITMGKEDKKTLRKPVKMQGEWNLDSNSEFQFKTELPFEKGSQVIEIDSPSFLGGDGNRLGPMAYCIAGITSCFVGTFVGIAANQGIKLTKLKVNTECNINFAKTFDLSEEPITEGINFKIDAQSENADNQKLQEILNMAEERCPAMYSMTHKINIDAKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ63</accession>
    <name>A0A2S2KQ63_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Geranylgeranyl pyrophosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_06030</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876453.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ63"/>
    <dbReference type="GeneID" id="76209956"/>
    <dbReference type="OrthoDB" id="26738at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43281">
        <property type="entry name" value="FARNESYL DIPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43281:SF1">
        <property type="entry name" value="FARNESYL DIPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01017">
        <property type="entry name" value="Polyprenyl_Transferase_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="326" mass="35669" checksum="2022AE0309E841F7" modified="2018-07-18" version="1">MKKTKQIEKNAKTVNRYLNSKLKGNPKKLYDAAGHLIVNGGKRLRPYMVIRSCQILGGKSSNAMPAASAVEMVHNFTLVHDDIMDNDEMRHGVPTVHKKFGMPIAILAGDVLFSKAFQVITDSKLSPNATIQLVSRLAKACVDVCEGQLLDVKMAEEEKIPTEAEYITMVSKKTAALFDVSCAMGAICATSRIKDISNLSSFGRNLGIAFQITDDLIGVMGDPKLTKKPVGNDLREGKKSLPILMAIKSAKGKDKKIILKAFGNSKISKNDLNKAVDVIRSLNIEENVRKQALKYADMAEKSLSTYSNPAKAELISLLDFVVKRSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ74</accession>
    <name>A0A2S2KQ74_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor Pcc1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_04620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CTAG/PCC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876332.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ74"/>
    <dbReference type="GeneID" id="76209814"/>
    <dbReference type="OrthoDB" id="11290at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015419">
        <property type="entry name" value="CTAG/Pcc1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011470">
        <property type="entry name" value="PRK14887.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09341">
        <property type="entry name" value="Pcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="9163" checksum="0CBBFB122A79BEA5" modified="2018-07-18" version="1">MTLNFSAKITVDAKDKTKAIFDSVNVDNDFYPENPVKTKIKFDKKITITAESDQLTHLRANLNSTLRLIQASYDSIESVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ82</accession>
    <name>A0A2S2KQ82_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleotidyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_04720</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876341.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ82"/>
    <dbReference type="GeneID" id="76209824"/>
    <dbReference type="OrthoDB" id="15372at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="239" mass="26993" checksum="7D06DB8AD3589E1B" modified="2018-07-18" version="1">MKAIILAGGKGTRGKPYTEYFPKAMSPINGKPLIDYVVKYLKSFSFIKEIIIISDFTGLGGQIKNYYENQEGVTFVQDSQSGTGGDLLHIENRLKGESEFVLWFVDNLCAIDLKKMKEQFVDKKSTACIATRSKRKEETGFAVVKDGIIQEFKEKPVMKLQHSECLGIYMLGIDVIKRIKAKAKQKEINLSYDILQQLSKEGKISAYDIGDVEWIDAESPMVLERNGKTVKKIIKQMGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ83</accession>
    <name>A0A2S2KQ83_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transketolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_06140</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transketolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876563.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ83"/>
    <dbReference type="GeneID" id="76209968"/>
    <dbReference type="OrthoDB" id="6779at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07033">
        <property type="entry name" value="TPP_PYR_DXS_TK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000129">
        <property type="entry name" value="Transketolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051157">
        <property type="entry name" value="PDH/Transketolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43825">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43825:SF1">
        <property type="entry name" value="TRANSKETOLASE-LIKE PYRIMIDINE-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="4">
        <location>
            <begin position="7"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="324" mass="35088" checksum="D7997139AA2858B3" modified="2018-07-18" version="1">MNDPIMTDMRSAYSKALVQLGKENPNVVVLGADTTDSLKTSDFGKEFPDRFFNVGIAEANLVTISAGLAVSGKISFASTYAIFLPGRAVDQIRNNVAYPSPPGKKGLNVKLVSSHGGLSVGPDGGSHQQIEDIAIMRVIPNFRVLIPADTVAVSKLTQLMAREYGPFYMRMARSKTPLIHSESQEFHIGKGITLRDGSDCTIVACGITVRMALEAADTLQHDGISCRVLDMFSIKPLDNELLEKAARETGCMVTTEEHNIFAGMGSAVAESVTESYPVPIKRIGAQDMFGESARDDEVPLLLEKHGITSFNIAKQVKEIRSKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ87</accession>
    <name>A0A2S2KQ87_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_06100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876460.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ87"/>
    <dbReference type="GeneID" id="76209963"/>
    <dbReference type="OrthoDB" id="7017at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="115" mass="13251" checksum="CBB562339AFDF3E9" modified="2018-07-18" version="1">MEGRININVSAIDYDKTSKALTRQLSLLEEMVHGEEDFTMTDSEFAFGWHFFVLSVNKSLVQKLAEMMGSDFDKLKGKGIEKKFLTWLTKNIESKSPRFKIAIKEEMESSKFGIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ94</accession>
    <name>A0A2S2KQ94_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NADH-quinone oxidoreductase subunit NuoH</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_06330</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876481.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ94"/>
    <dbReference type="GeneID" id="76209990"/>
    <dbReference type="OrthoDB" id="15253at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01350">
        <property type="entry name" value="NDH1_NuoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001694">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018086">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004741">
        <property type="entry name" value="PRK06076.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11432">
        <property type="entry name" value="NADH DEHYDROGENASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11432:SF3">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00146">
        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00667">
        <property type="entry name" value="COMPLEX1_ND1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00668">
        <property type="entry name" value="COMPLEX1_ND1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="217"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="310"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="368"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="406"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="432" mass="46806" checksum="50DA47441CC54120" modified="2018-07-18" version="1">MSVIAPKFKLSEFIKSLLDNAFWILFLLVLVGIPAVFMVLFAIEMPVINGELLTPFLALTWIADPSRTLPIIKAFMATDIFRVMAFPGFGFAALLAAGTIFVERKMLAKLQLRVGPFYCGKVEGILQLMGDGLKLISKEIIIPAKADKPIFWAAPVMFVGAAAAFVALIPVAPGWVVADVDLGLLGVFAVIGFFPIITILSAWSANSKFPFIGGIRALFQMVSFEIPLILSLLGVVILTGTLNLSEIAASQSSFPWIVFLPVGAVVFFITMLAELERIPFDLPEAESEIVAGWLTELSGMMYGLVQLGTYLKLYAFAALFVVLFLGGWNGPMVVPPFPAEILTDGIVMGPITAKIPGLPVFTQEMLNGTLWFILKTVGVIFFILLPRGVFPRIRIDMLLSLGWTKLIGLAFVNIFIALGLLYAGVLGPGGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ98</accession>
    <name>A0A2S2KQ98_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_04920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866821.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ98"/>
    <dbReference type="GeneID" id="76209844"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="119" mass="12981" checksum="4EC6E762AF3C88DE" modified="2018-07-18" version="1">MVQIKQQEGRLSRRVLLSAMIAGIAVAVVFTVTPWNYVPTEVTENVTVIAVTEHGCVGESQYGMSVVVSDCSAQVGDTVSATFNVPSGLLNGYWDELERRQNPMVDIWDRNVDGSGFSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQA1</accession>
    <name>A0A2S2KQA1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PEFG-CTERM sorting domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_05750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQA1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027560">
        <property type="entry name" value="PEFG-CTERM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04296">
        <property type="entry name" value="PEFG-CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="278" mass="29446" checksum="D6A75D3911663466" modified="2018-07-18" version="1">MTAVGTLTMSSAYAVELPPACVGCAQDEAKASANKMLMAAIPISVWTDKTEYNHNEMIMVSGQVANIASGYPVTVTVVSPLNSIVTVNQITVAEDKSFETMLNTAGAMWKYDGTYTIKVNYGSVEKSNSAKVELTGGVVTTPTPSKCGVNEITASGQCIPFSISGGVVKSATINTDDKSIVINIDAKKDGTLTVTPSKKIQDGIFMVLVDGEQWDDVEIDKARNKVTVMFLAGAEKIEIIGTFVIPEFGTIAAMILAVAIISIIAVSAKSRLSIMPRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQE1</accession>
    <name>A0A2S2KQE1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_06600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQE1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052048">
        <property type="entry name" value="ST_Response_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228:SF1">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ARR22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="1">
        <location>
            <begin position="4"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="124" mass="13774" checksum="9AAA94B39D004FFE" modified="2018-07-18" version="1">MGLSALVVDDSKFMRESIRETLSYLKIGSIIEAKNGIEGVRAFIKHRPSLVTLDYEMPGLNGIETAIKIREIDKNVMMIIVTSIKSNMIAVKSGKIPNLGYVTKPIDPIMIKEALSKLQKQDVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQF5</accession>
    <name>A0A2S2KQF5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH:ubiquinone oxidoreductase 30kDa subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_06350</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 30 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876482.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQF5"/>
    <dbReference type="GeneID" id="76209992"/>
    <dbReference type="OrthoDB" id="43567at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.80">
        <property type="entry name" value="NADH:ubiquinone oxidoreductase, 30kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037232">
        <property type="entry name" value="NADH_quin_OxRdtase_su_C/D-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001268">
        <property type="entry name" value="NADH_UbQ_OxRdtase_30kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020396">
        <property type="entry name" value="NADH_UbQ_OxRdtase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10884:SF14">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10884">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00329">
        <property type="entry name" value="Complex1_30kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143243">
        <property type="entry name" value="Nqo5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00542">
        <property type="entry name" value="COMPLEX1_30K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase 30kDa subunit" evidence="4">
        <location>
            <begin position="63"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="195" mass="22214" checksum="64FCACE1A353AD56" modified="2018-07-18" version="1">MSSDLEKPSVDVKPEEKSTPPTKKPEEKPADLPAFEKGISEKIIEKFGDKVQVAFVKVNRVGINVSRENVHEVAEFIRDGLNYDHVESVSGVDYPQDKEIEVVYHIGSYTDSSLASQILVLSTRAQREENPIPGNDATKLPTLRDVFYSVEFHEREIFEMFGVYFEGHPDNRRLLLPEDWADLPPLRKDFAIKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQG7</accession>
    <name>A0A2S2KQG7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_06970</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_263970058.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQG7"/>
    <dbReference type="GeneID" id="76210057"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="44" mass="5461" checksum="F5A27B1A9397ADB7" modified="2018-07-18" version="1">MVNQIENRMLFHDEELDEQDLQKFFEKLDSTTVEQIKRLYEKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQH9</accession>
    <name>A0A2S2KQH9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_07310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876593.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQH9"/>
    <dbReference type="GeneID" id="76208944"/>
    <dbReference type="OrthoDB" id="4827at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="217" mass="24714" checksum="B8CC9CD99AE6C42B" modified="2018-07-18" version="1">MAVFKKNPPDYLLHPFLEFLIVTLSLVAILVPLRILSKVIFDDEWIGSIGVITVVFGVILFLSMKEKLGNFGQMFLRQITKNHTRKQKWIIFSQTGLFLSLGILTIFSIHAGNTEYHLLKEQVIAEFGNKGIMIDSDLNFDAINQISSQVSPEQQVGAIAALPLLTIQNFEIFSVVLAVTDQMMGGWVMYFWQIMVIETMEISIFLFITRKFTLKYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQK6</accession>
    <name>A0A2S2KQK6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">isopentenyl-diphosphate Delta-isomerase</fullName>
            <ecNumber evidence="3">5.3.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">NZNM25_06020</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IPP isomerase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.2" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876452.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQK6"/>
    <dbReference type="GeneID" id="76209955"/>
    <dbReference type="OrthoDB" id="4234at2157"/>
    <dbReference type="UniPathway" id="UPA00059">
        <property type="reaction ID" value="UER00104"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004452">
        <property type="term" value="F:isopentenyl-diphosphate delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050992">
        <property type="term" value="P:dimethylallyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009240">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02885">
        <property type="entry name" value="NUDIX_IPP_Isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00202">
        <property type="entry name" value="Idi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056375">
        <property type="entry name" value="Idi_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011876">
        <property type="entry name" value="IsopentenylPP_isomerase_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02150">
        <property type="entry name" value="IPP_isom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002995">
        <property type="entry name" value="PRK03759.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885:SF0">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018427">
        <property type="entry name" value="Isopntndiph_ism"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="10">
        <location>
            <begin position="30"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH33811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="216" mass="25007" checksum="A1D54AC7C94F7DB5" modified="2018-07-18" version="1">MTDEFVILVDENDNPIGSEEKVKCHLPNGKLHRAFTALLFDQDGRLVLTRRAKEKMLWPKDWDGTFASHPRESETYVSSGERRMPEELGITGTLDYLHKFEYHVPYKDIGSENEICGTLVGVIDKSTELKEVEGEIDEIKWISSKELLSELNTNPQNYCPWMLIALELLEKSDQSMLEKYANILTTWMNSEVHEGLQKAIETHLPKEKWRLVNEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQL5</accession>
    <name>A0A2S2KQL5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07530</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876613.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQL5"/>
    <dbReference type="GeneID" id="76208923"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="90" mass="10050" checksum="0562D4BB651431BE" modified="2018-07-18" version="1">MATWKLYVENNNSLNEKWIQKANESISNKSTKLDNFEVDDSQLSASLNYLEKCGYNEIKLCVVEKRIVLPPSNSSPCPNCKGSGHVKSGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQR7</accession>
    <name>A0A2S2KQR7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_05890</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829043.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQR7"/>
    <dbReference type="GeneID" id="76209941"/>
    <dbReference type="OrthoDB" id="4at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="50" mass="5840" checksum="8269A5C7180FEDBA" modified="2018-07-18" version="1">MQKLCNYDPSGKGEHCFCIDIDTQRGIKKCCKCTIWNVQGNDWTEDEDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQS8</accession>
    <name>A0A2S2KQS8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQS8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="133" mass="14358" checksum="3031C6D6141D0812" modified="2018-07-18" version="1">MGGMFSSVNFAINGGSVMPEVSILDDGLLPWLNEFVSANGGATFLIITSITLMASATKRIVGMNSGFLNRMVTFVGTFVFMTILVLSFTKSDPTSSGVFLYTFYHAGIVGGAFYAMNKLTKNQNMIEDFTNGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQT1</accession>
    <name>A0A2S2KQT1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ParB-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_07650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876625.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQT1"/>
    <dbReference type="GeneID" id="76208911"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1530.10">
        <property type="entry name" value="Conserved hypothetical protein from pyrococcus furiosus pfu- 392566-001, ParB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="ParB/Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB-like N-terminal" evidence="1">
        <location>
            <begin position="48"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="331" mass="38519" checksum="470CD349C3433EB9" modified="2018-07-18" version="1">MKSILKEIPVEAIQLDDENPRLRFSKIERGIKNWTQENMMEEIQDSPVFNRLFDSIHEYGVIDPIWVYDLANGSYQVIEGNMRVTVLRELIKKKIPSSGGINYKSVRAHVVPNSTSQVDLEVQKAILQTGKTPWGAFNEAAHIYNLFWKNKISIEDIASMLGKSRSYIDNEIDNFKFYLEFIEYGKKKGDYEVDPKKYSFFKDAPGTIKQKFFKSPVSRKNYFELITPNKNGITRIPSVSLKGGLRTFGKIVEDDAILKKFLNDARMTVDDAFLEFTEKNYLSKLPWIKKIPSVSKSMNKLNKFERKKIFENPESKKTLTDLYKELKKFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQW0</accession>
    <name>A0A2S2KQW0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08300</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876676.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQW0"/>
    <dbReference type="GeneID" id="76208843"/>
    <dbReference type="OrthoDB" id="11617at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="82" mass="9177" checksum="E197A8F299B955EC" modified="2018-07-18" version="1">MKEPHNYAKVGYSMIVVSASLAAVGLLALAIGSDVLFADNIQRENTHHFNECKLNDFKTEGCEKYWDRINNEISGIYVDLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQX0</accession>
    <name>A0A2S2KQX0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_07220</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876587.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQX0"/>
    <dbReference type="GeneID" id="76208953"/>
    <dbReference type="OrthoDB" id="9652at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00156">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050595">
        <property type="entry name" value="Bact_response_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591:SF3">
        <property type="entry name" value="RESPONSE REGULATORY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591">
        <property type="entry name" value="STRESS RESPONSE REGULATOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="4"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="122" mass="13726" checksum="51E2C91FB8855B1D" modified="2018-07-18" version="1">MAKKILVAEDNKFTATQYAKILESENHQVEIVKDGEECIQKYAKSINADSKKSTFDVLILDHSMPQRNGAEVAGDILSLNPEQKIILASAYALSTERNFSKLKDKVLFLQKPFQLSKILELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQY5</accession>
    <name>A0A2S2KQY5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_06590</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQY5"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052048">
        <property type="entry name" value="ST_Response_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228:SF1">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ARR22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="1">
        <location>
            <begin position="20"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="143" mass="16111" checksum="64302A34EB0CAAB3" modified="2018-07-18" version="1">MLIDVEKMSMKTMNPLRFCKILIVDDSAYMRTFIKRTLSDAQIGSRYYEANDGKEAISKYVAFKPDVTIMDIIMPNVDGVKASMAINHYDPNAKIIVISAKENKETVEDVVKKGGAKDYILKPCDSSAIVMSVSKQIVMNRHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQZ0</accession>
    <name>A0A2S2KQZ0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HTH arsR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_06560</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876496.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQZ0"/>
    <dbReference type="GeneID" id="76210013"/>
    <dbReference type="OrthoDB" id="11368at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051081">
        <property type="entry name" value="HTH_MetalResp_TranReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33154">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ARSR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33154:SF35">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ARSR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="HTH arsR-type" evidence="5">
        <location>
            <begin position="25"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="226" mass="24924" checksum="2ECFF48D7B53AA5D" modified="2018-07-18" version="1">MNSDDIPDLSDRVDILSTEDEKLKTIGEILSSDSSRNILKILFNESLTANQIAQRTEMSLPLVIHHLKKMQSAQVIKITNVGKNSKSHDMKYYTIDKFAIVILPTAMTQSAKKSKSLFNSFTRIHRLATLGGVSIAAWFSSQFLQNQTSIPVVQSKVSSKISESSESMMMRSIPEQSENIQDALPSSSSDPQIGVLLSVVVVLSVIVSGLIIELIIKQKLNAAKYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQZ5</accession>
    <name>A0A2S2KQZ5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08700</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876711.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQZ5"/>
    <dbReference type="GeneID" id="76208800"/>
    <dbReference type="OrthoDB" id="5997at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="60" mass="7050" checksum="96C354ED2E65478D" modified="2018-07-18" version="1">MSEHKYSKWTVEGDKKTKWICGCFQTSDNYFKFCDTHSITLQKAIQAQIDELDMTLIVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQZ6</accession>
    <name>A0A2S2KQZ6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Carboxyvinyl-carboxyphosphonate phosphorylmutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">bcpA</name>
        <name evidence="1" type="ORF">NZNM25_06690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQZ6"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016833">
        <property type="term" value="F:oxo-acid-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00377">
        <property type="entry name" value="ICL_PEPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000009">
        <property type="entry name" value="2-methylisocitrate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039556">
        <property type="entry name" value="ICL/PEPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018523">
        <property type="entry name" value="Isocitrate_lyase_ph_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42905:SF5">
        <property type="entry name" value="CARBOXYVINYL-CARBOXYPHOSPHONATE PHOSPHORYLMUTASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42905">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13714">
        <property type="entry name" value="PEP_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00161">
        <property type="entry name" value="ISOCITRATE_LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="288" mass="31672" checksum="F3773CF7132C8990" modified="2018-07-18" version="1">MKNLKSMFKSKKPLVIPGVYDAIGAKIVEKVGFDAMFQTGYGTSATLFGMPDYGFIGASETVDNARRICRAVSVPVIVDSDTGYGNALSVWKLVKELESSGASGIFLEDQRWPKRCGHMQGKEVIPQEEYTEKLGAAIDARESKDFIIVARTDARATEGLDAAIERGIQNKKTGADAVFIEAPKTLDEMKKIGKAIKAPLVANMIEGGATPLSSAEALNKMGFKIILYPLSVLFANTFATMNILQELKKSGSTTKFKQKVVNFDQFNDLVELPKFRKMEKKYGFSKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR01</accession>
    <name>A0A2S2KR01_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Double-stranded DNA repair protein Rad50</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_09010</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876739.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR01"/>
    <dbReference type="GeneID" id="76208768"/>
    <dbReference type="OrthoDB" id="25344at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.510">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114:SF2">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75712">
        <property type="entry name" value="Rad50 coiled-coil Zn hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad50/SbcC-type AAA" evidence="3">
        <location>
            <begin position="5"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="200"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="430"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="806" mass="92877" checksum="53F7CAFDD7FF6AA9" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR12</accession>
    <name>A0A2S2KR12_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_09110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876747.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR12"/>
    <dbReference type="GeneID" id="76208759"/>
    <dbReference type="OrthoDB" id="875at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046600">
        <property type="entry name" value="DUF6659"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037240">
        <property type="entry name" value="ORC1-binding_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20364">
        <property type="entry name" value="DUF6659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144005">
        <property type="entry name" value="ORC1-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="120" mass="14011" checksum="64311CA9FB177913" modified="2018-07-18" version="1">MNYEKFCSEILDADPKIRFATVYDEWAVRVAGGMREGIENLLSDHAEKELVNLSIMDWKARKDMSKWLGMTKYTLGEYDKVKRFSFYLGDDHLLLVSAEKDSDTNVVVDEVIKLYYKNQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR27</accession>
    <name>A0A2S2KR27_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cupin type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_07820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876637.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR27"/>
    <dbReference type="GeneID" id="76208893"/>
    <dbReference type="OrthoDB" id="190812at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02208">
        <property type="entry name" value="cupin_RmlC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-2" evidence="1">
        <location>
            <begin position="34"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="106" mass="12307" checksum="4A97936960E08ED8" modified="2018-07-18" version="1">MKLDYDLNSYIEKIKKSNSYFHTFINRESLAAGILVLQPNEEDTQEPHNSDEVYYVISGDGFLKIKNNDYKISKDKLFFVAKGVEHYFHGNTKELKVLYFFGGPDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR39</accession>
    <name>A0A2S2KR39_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Flagellar accessory protein FlaH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_07090</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876576.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR39"/>
    <dbReference type="GeneID" id="76208966"/>
    <dbReference type="OrthoDB" id="63735at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004723">
        <property type="entry name" value="PRK06067.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637:SF3">
        <property type="entry name" value="FLAGELLA-RELATED PROTEIN H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="22"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="233" mass="25355" checksum="3A923672457C6C8E" modified="2018-07-18" version="1">MLNIISTGNEEIDRQFGGGIPSPSLVFIEGAHGTGKSAISAQIMNGLLTSKKSLLCVIENTVKQHIQKMKSITFNFSKPFVRSQLIFVPMYIKDAKWTNNNTEKILPAIKEFILEKIDKVDGVIIDSISPMVNNSKNESILDFLSFCKEIVAMGKTIIITSHSSDFSQATNTAIIGTTDVYIKLGTVVVGDKEVKTLKIIKILGAADTPEAGFAFEVDLIFGIKIVPISMANA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR48</accession>
    <name>A0A2S2KR48_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">tRNA-t(6)A37 methylthiotransferase</fullName>
            <ecNumber evidence="11">2.8.4.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">NZNM25_09200</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:37075"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="Rhea" id="RHEA-COMP:11092"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:74420"/>
            <dbReference type="EC" id="2.8.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="11">Binds 1 or 2 [4Fe-4S] cluster. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the methylthiotransferase family. CDKAL1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.5" evidence="11"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876755.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR48"/>
    <dbReference type="GeneID" id="76208750"/>
    <dbReference type="OrthoDB" id="372134at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035598">
        <property type="term" value="F:tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.30.20:FF:000002">
        <property type="entry name" value="threonylcarbamoyladenosine tRNA methylthiotransferase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="entry name" value="Methylthiotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006466">
        <property type="entry name" value="MiaB-like_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01578">
        <property type="entry name" value="MiaB-like-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00089">
        <property type="entry name" value="MiaB/RimO family radical SAM methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11918">
        <property type="entry name" value="RADICAL SAM PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11918:SF45">
        <property type="entry name" value="THREONYLCARBAMOYLADENOSINE TRNA METHYLTHIOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 11 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="MTTase N-terminal" evidence="12">
        <location>
            <begin position="2"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="13">
        <location>
            <begin position="131"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GBH34129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="422" mass="46555" checksum="8ED4B0AB47B1FBB1" modified="2018-07-18" version="1">MAKIYVEAYGCSASFADSEMISGLIQNGGHTLVGSSSESDLNIVVTCSVKDSTANKMMHRIKSLKTKPLIVAGCLPKAEQSVVEKFSEKASLLGPNSLGKTIQIINSTLKGRKQIALEDSDLSKVGLPKVRLNPTVGIVEIASGCLSECTFCQTKLSKGDLSSYRLGDIVRQIQTEIKEGCKEVWLTSTDNGCYGFDIGTDLPSLIDAVVEIPNNFMVRVGMMNPMYMPRIKDKLIKSFDNNKVFKFLHIPVQSGSDKVLHDMKRGHTSGTFRDIVKKARERFEQFTISTDIIVGFPTETEEDFQETIALLDETKPDVVNLSRFSARPGTDAAELKQLDAAEVKRRTKIIFEQISKLSMESNKKWIGWKGRVLFDENTDEGIKGRNFAYKPVAVADKVDIGDSHIVEITDASVKRLVGKIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR79</accession>
    <name>A0A2S2KR79_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AF1548-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_09570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR79"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054374">
        <property type="entry name" value="AF1548-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22357">
        <property type="entry name" value="AF1548-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AF1548-like C-terminal" evidence="1">
        <location>
            <begin position="165"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="224" mass="26233" checksum="7CDB837D030D2079" modified="2018-07-18" version="1">MTSNDIYKIVLRVISEEKDLHALHQRYQLKDAIMRMGPAGFAFENYIASILEYYDYQVIRIRSKIRGKCATHEIDLIGMKNNQEFLIECKYHSLRGVYTGLKESLYTHARFLDMHPKFAGEIIFCNTQVSNHAKKYAKCIGQKIFSWRYPAANSLEKIIEKHNLYPITILNLTENELRIFSDCNMMVAKDLLRYDEAKIAKMTGISKKRINNMQKLVKQIFHPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR99</accession>
    <name>A0A2S2KR99_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1059 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_09600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876791.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR99"/>
    <dbReference type="GeneID" id="76208710"/>
    <dbReference type="OrthoDB" id="9023at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009409">
        <property type="entry name" value="DUF1059"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06348">
        <property type="entry name" value="DUF1059"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="61" mass="6951" checksum="736BD8657D4FBB0E" modified="2018-07-18" version="1">MVKSFYCEDMGKDCNWSAKANTTSELMKKIIEHADTKHGIKQIPVHRKAKLASAIKDDKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRC4</accession>
    <name>A0A2S2KRC4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_09080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876745.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRC4"/>
    <dbReference type="GeneID" id="76208762"/>
    <dbReference type="OrthoDB" id="9652at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052048">
        <property type="entry name" value="ST_Response_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228:SF1">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ARR22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="1">
        <location>
            <begin position="9"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="194" mass="21596" checksum="FA730657722A268A" modified="2018-07-18" version="1">MANPLRFSKILIVDDSEAFRLKVKSILIDAQIGYYFYEARDGKEAVEQYISKKPHVVIMDILMPNVDGLTAIAAIRKYDPNAKIIVASTKDNKELVDDAIKTGGAKDFIFKPFNSGVVVMAVSKQLLMKKETTKPANNSTKKPLMYNGVTYGSVNGISNDGEFVLSVNKDDEADKIVSQMQDIKDMGNYYEIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRC5</accession>
    <name>A0A2S2KRC5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Crotonase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">crt2</name>
        <name evidence="4" type="ORF">NZNM25_09550</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876788.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRC5"/>
    <dbReference type="GeneID" id="76208715"/>
    <dbReference type="OrthoDB" id="27846at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000009">
        <property type="entry name" value="Carnitinyl-CoA dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF54">
        <property type="entry name" value="ENOYL-COA HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="253" mass="27194" checksum="7886BF1D44F0A93D" modified="2018-07-18" version="1">MSLVTTSTSDGICTVKINRPDKLNAMNIDVAKELIKTFEELNHDDDVKVIILTGEGEKAFSAGADIEYMSKISADESVDYAKTGQLVTSTVELVKQPTIAAINGFALGGGCELAMSCDIRIAADTAKLGQPEVTIGIPPGWGGTQRLMRIVGIAKAKELVYTGKMIKADEAKEIGLVNHVVPLASLQEEALKMAQQIAGNSIMGVQMSKVAINKGRNADLDTGLAVELLAWRNCFTHPDREERMTAFVNKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRG3</accession>
    <name>A0A2S2KRG3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GIY-YIG nuclease family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRG3"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="170" mass="19427" checksum="FA7F7108D4688DBD" modified="2018-07-18" version="1">MKSSALSIQILQYEFLGPIPLDEWGPPMEKLVYIILSKDQDKFNILYVGDCEKTDDKSFFVQNPDFKCWVQKSGSEKSLYLAILPLFESSKERRQSVLNKIFSRYKPPCNSTEPPKAEPDYVVRKSEINSEDQKISCPCCGSEMKAEQILEKSTVYRCTSCGLSDTRLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRI5</accession>
    <name>A0A2S2KRI5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">S-methyl-5'-thioadenosine phosphorylase</fullName>
            <ecNumber evidence="5">2.4.2.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">5'-methylthioadenosine phosphorylase</fullName>
            <shortName evidence="5">MTA phosphorylase</shortName>
            <shortName evidence="5">MTAP</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mtnP</name>
        <name evidence="7" type="ORF">NZNM25_10730</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>S-methyl-5'-thioadenosine + phosphate = 5-(methylsulfanyl)-alpha-D-ribose 1-phosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:11852"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="EC" id="2.4.2.28"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 5">Homohexamer. Dimer of a homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.28" evidence="5"/>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877016.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRI5"/>
    <dbReference type="GeneID" id="76208586"/>
    <dbReference type="OrthoDB" id="7681at2157"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00873"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017061">
        <property type="term" value="F:S-methyl-5-thioadenosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09010">
        <property type="entry name" value="MTAP_SsMTAPII_like_MTIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1580:FF:000012">
        <property type="entry name" value="Probable 6-oxopurine nucleoside phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01963">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010044">
        <property type="entry name" value="MTAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018099">
        <property type="entry name" value="Purine_phosphorylase-2_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01694">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006334">
        <property type="entry name" value="PRK08564.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679">
        <property type="entry name" value="S-METHYL-5'-THIOADENOSINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679:SF3">
        <property type="entry name" value="S-METHYL-5'-THIOADENOSINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01240">
        <property type="entry name" value="PNP_MTAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3 5" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="6">
        <location>
            <begin position="7"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="55"/>
            <end position="56"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="88"/>
            <end position="89"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="210"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="5">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="5">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00063054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="263" mass="29222" checksum="88059C0EACBECD1F" modified="2018-07-18" version="1">MEKDVEIGIFGGTGIYDSGLLENPQEIDIDTPYGKPSDTITVGIFNGRKIAFLPRHGKKHTIPPHMINFKANIWAFKELGITRIIAPSAVGSLKEEVEPGHFALPSQFLDFTKSRNGSFSEEGKVIHISVADPFCPELQASILKVTDNEDLKMHKDCTYVCIEGPRFSTKAESKFYRTTGADIIGMTLVPECQLAREAQMCYASISTVTDYDVWAEKPVTAKEVLETLSKNVEKTKKVLTELIEQIPKTRNCSCAKALEEAEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRJ1</accession>
    <name>A0A2S2KRJ1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">HAMP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">NZNM25_08660</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRJ1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06225">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.500">
        <property type="entry name" value="HAMP domain in histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033479">
        <property type="entry name" value="dCache_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089:SF112">
        <property type="entry name" value="LYSOZYME-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089">
        <property type="entry name" value="METHYL-ACCEPTING CHEMOTAXIS PROTEIN MCPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02743">
        <property type="entry name" value="dCache_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158472">
        <property type="entry name" value="HAMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="10">
        <location>
            <begin position="320"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="9">
        <location>
            <begin position="399"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="581" mass="65204" checksum="A8E6FCD2D9580D05" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRN4</accession>
    <name>A0A2S2KRN4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription regulator AsnC/Lrp ligand binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_10180</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876839.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRN4"/>
    <dbReference type="GeneID" id="76208649"/>
    <dbReference type="OrthoDB" id="8136at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="2">
        <location>
            <begin position="6"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="123" mass="13880" checksum="B0DB8CD2F2ADABA1" modified="2018-07-18" version="1">MDKAFVLINCDPGKEQSILGQLRKINNVVDAQGTYGVYDIVARIESDSKSQLDNIISDQIRKLDHVNSTLTLIPIDEQNIIPDLIPDVVPDVIPEQKKPLDESSEDEEEDLDENDYDDETKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRN5</accession>
    <name>A0A2S2KRN5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">TatD family hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_11070</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. TatD-type hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRN5"/>
    <dbReference type="OrthoDB" id="26412at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01310">
        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46317">
        <property type="entry name" value="HYDROLASE OF PHP SUPERFAMILY-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46317:SF1">
        <property type="entry name" value="HYDROLASE, TATD FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005902">
        <property type="entry name" value="DNase_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR005902-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="272" mass="30929" checksum="8CA063E45250CC49" modified="2018-07-18" version="1">MSEKISLRNRFIQTILRIDKEMTWYIDSHIHLSDSTYASDIGFILEEMKYLKIKACCVSTNVENSLQTLKLAQKSNLVLPFIGIHPECANDDLEKMINLIENNFTHISGIGEIGLDPTYVHSPEEEKKQKHVFETLLSIAEKFNKPVSIHSRKSLDDVFEIMTSFSTKHALLHWFDGSKKQLTKAMDMGFFVSYGPVMVYANDKQTLVSVTDESKILVETDGPVKFSRCFEMKSGQVTFIPSVIFCASKILGKTFDEMSFLLEKNSNSFLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRP2</accession>
    <name>A0A2S2KRP2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">AsnC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_014963852.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRP2"/>
    <dbReference type="GeneID" id="64829065"/>
    <dbReference type="OrthoDB" id="8136at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF6">
        <property type="entry name" value="REGULATORY PROTEIN ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="1">
        <location>
            <begin position="6"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="79" mass="8824" checksum="FF93DCB88F11C45A" modified="2018-07-18" version="1">MATAYVLINCELGSEEAIIQQLKGLEGVKEVHGTFGAYDILAKIESDTVEKLRETITWKIRKIEKIRSTLTLMGIEGQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRW6</accession>
    <name>A0A2S2KRW6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Tryptophan--tRNA ligase</fullName>
            <ecNumber evidence="2 9">6.1.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">NZNM25_11450</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>tRNA(Trp) + L-tryptophan + ATP = L-tryptophyl-tRNA(Trp) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24080"/>
            <dbReference type="Rhea" id="RHEA-COMP:9671"/>
            <dbReference type="Rhea" id="RHEA-COMP:9705"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78535"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="2 9"/>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876986.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRW6"/>
    <dbReference type="GeneID" id="76208511"/>
    <dbReference type="OrthoDB" id="371821at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006436">
        <property type="term" value="P:tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00806">
        <property type="entry name" value="TrpRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.240.10:FF:000007">
        <property type="entry name" value="Tryptophan--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008927">
        <property type="entry name" value="PRK12285.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10055:SF1">
        <property type="entry name" value="TRYPTOPHAN--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10055">
        <property type="entry name" value="TRYPTOPHANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="NCBIfam" id="TIGR00233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="370" mass="42376" checksum="46A1239F4C70E7FF" modified="2018-07-18" version="1">MSADDFIVTPWHVEGDIDYDKLIKKFGTERISSELLGRIRTVVGEDHFMLRRGIFFSHREMNRILDDYQKGQKFFLYTGRGPSGHTHIGHLVPWVFSKWLQEKFDTNMYFQLTDDEKFFSKSNLTLEETNKFAYENALDFIALGFKSDKTKIIINTKNIQTLYPIAAQVAKKINFSNTKATFGFTNETNIGMIFYTALQSAPCFIENKPVLIPLGVDQDPHFRLTRDIAPKIGKPKPALIHNIMIPSLEGPGGKMSASDNNGTIYTTDSPNVVKKKINKHAFSGGQPDIEQHRKIGGNPDIDVAYQYLRIFFEPDDNKLKAIYEDYKSGKLLSGELKAILIEKINDFLSIHQEKREKAKDKIHEFLLENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS12</accession>
    <name>A0A2S2KS12_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 15">tRNA-splicing ligase RtcB</fullName>
            <ecNumber evidence="15">6.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">rtcB</name>
        <name evidence="17" type="ORF">NZNM25_11220</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential for tRNA splicing and maturation. Acts by directly joining spliced tRNA halves to mature-sized tRNAs. Joins RNA with 2',3'-cyclic-phosphate or 3'-phosphate ends to RNA with 5'-hydroxy ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP + H2O = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68080"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:68076"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14 15">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="14 15">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 15">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 15">Belongs to the RtcB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.-" evidence="15"/>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876967.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS12"/>
    <dbReference type="GeneID" id="76208535"/>
    <dbReference type="OrthoDB" id="9887at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003972">
        <property type="term" value="F:RNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170057">
        <property type="term" value="F:RNA ligase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1860.10:FF:000001">
        <property type="entry name" value="tRNA-splicing ligase RtcB homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1860.10">
        <property type="entry name" value="tRNA-splicing ligase RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001233">
        <property type="entry name" value="RtcB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036025">
        <property type="entry name" value="RtcB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118">
        <property type="entry name" value="RNA-SPLICING LIGASE RTCB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118:SF1">
        <property type="entry name" value="RNA-SPLICING LIGASE RTCB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01139">
        <property type="entry name" value="RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103365">
        <property type="entry name" value="Hypothetical protein PH1602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 15 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 14 15" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="active site" description="GMP-histidine intermediate" evidence="12">
        <location>
            <position position="405"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="204"/>
            <end position="208"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="330"/>
            <end position="331"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="379"/>
            <end position="382"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="405"/>
            <end position="408"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="481"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU371113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GBH34331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="482" mass="52814" checksum="550569F4ECE4BDF1" modified="2018-07-18" version="1">MSSTTPRKIGENQYQIDVDSNFGMKVPVRIYADEPLLQKMLSDRTIMQARNVASIPGIVGHSVVLPDGHEGYGFPVGGVAAMDAEEGMISPGGVGYDINCGVRLLRSNLDEQTVRSKLKELVNDLFSSIPSGVGSKGAVKLTHSELDEVLVKGVNWAIDHGYGSSNDSDVCEENGQIQNADPNKVSDKARKRGAPQLGSLGSGNHFLEVQKVSEIHDEEAANRMGIKEGTITVLIHCGSRGFGHQVCSDYLRVSEQAMQKYDIDLPDRELACVPNNSEEGESYRKAMFAALNFAWSNRQMLTHWTRNSFERVFHQTESDLDMKLVYDVAHNIAKVEKHNVDGKERKLVIHRKGATRAFPANRQEIPSKYRDLGQPVLVPGSMGTSSWILLGQPNSMNLSFGSTAHGAGRTMSRSKARRNYTENDVKKSLNDQGIFIKALTRDGVVEETPQAYKDVDAVVNVSHNLGIATKVAKLVPIGVIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS53</accession>
    <name>A0A2S2KS53_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12700</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877106.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS53"/>
    <dbReference type="GeneID" id="76209231"/>
    <dbReference type="OrthoDB" id="2694at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="140" mass="16141" checksum="3B8126346D620DA6" modified="2018-07-18" version="1">MPLDKMPDVEQYVPTHKSTVLHVKGKPVACIIDDISNELRFKSVSKNNSLKASLIGFLNKHDDLGLLLGFKLKIQTDLENFEYTVYPNDEFIETVIFDESVFIINKKLEPLFSLKKIVTDQFVKTKTEFDKFQSLINKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS57</accession>
    <name>A0A2S2KS57_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tryptophan synthase alpha chain</fullName>
            <ecNumber evidence="9">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">trpA</name>
        <name evidence="11" type="ORF">NZNM25_12930</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the TrpA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="9"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877121.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS57"/>
    <dbReference type="GeneID" id="76209207"/>
    <dbReference type="OrthoDB" id="25658at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04724">
        <property type="entry name" value="Tryptophan_synthase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000037">
        <property type="entry name" value="Tryptophan synthase alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00131">
        <property type="entry name" value="Trp_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018204">
        <property type="entry name" value="Trp_synthase_alpha_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002028">
        <property type="entry name" value="Trp_synthase_suA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00262">
        <property type="entry name" value="trpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43406:SF1">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE ALPHA CHAIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43406">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE, ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00290">
        <property type="entry name" value="Trp_syntA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00167">
        <property type="entry name" value="TRP_SYNTHASE_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="267" mass="29201" checksum="FC5CD427F394BBA2" modified="2018-07-18" version="1">MSKIKEKFAELEKKNQKALVSYIMAGFPDEKATISTVRGLVKGGADIIELGFPFSDPLADGPVIQNASTVSLQKGAKINKFFTLVKKIRKETNIPLVLMTYTNILYHKGYSKFIKDAKNAGIDGFILPDMSIEESKEYTHAAKNNADTVFLISPNTTKKRIEKISKASTGFLYLVAVYGTTGIKTGIKNYTLKAIKDVKKQTGGKIPIGVGFGVSTPDDVRKYIDAGADAVIVGSAYLKLIENTPQNKLESKIAAFTKKLKKQTIPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSA1</accession>
    <name>A0A2S2KSA1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_11260</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876969.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSA1"/>
    <dbReference type="GeneID" id="76208531"/>
    <dbReference type="OrthoDB" id="43333at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="13"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="77"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="153" mass="16644" checksum="C938C7483690138F" modified="2018-07-18" version="1">MTDAKTTTIADVMTKSVISVDASLTVNETAKMMEDSKVGAVIIMEDNTPIGIVTDRDFAVKVAAHAYQISTPIKQIMSSPLFSINSDESVRAAADLMHDRGIRKLPVIDDGNIVGIITASDIVNLLAVCVEEDIKDMYFHSVAKIYSNYSPYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSB6</accession>
    <name>A0A2S2KSB6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Methylmalonyl Co-A mutase-associated GTPase MeaB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_13610</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIMIBI class G3E GTPase family. ArgK/MeaB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSB6"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052040">
        <property type="entry name" value="GTPase/Isobutyryl-CoA_mutase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005129">
        <property type="entry name" value="GTPase_ArgK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00750">
        <property type="entry name" value="lao"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43087:SF1">
        <property type="entry name" value="LAO_AO TRANSPORT SYSTEM ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43087">
        <property type="entry name" value="LYSINE/ARGININE/ORNITHINE TRANSPORT SYSTEM KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03308">
        <property type="entry name" value="MeaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="305" mass="33108" checksum="23B48430C6ADB46A" modified="2018-07-18" version="1">MDMLADLKKGKRGAIAKAITIVENDQKEAKKLIKKIFKDTGDSIIIGITGPAGAGKSSLINKTTVEMKKLGTKPAVLAIDPTSHVTGGAILGDRVRMSESTDSGTYIRSIASRGATGAVSRSLRNSIRILEYAGFNPIIIESVGAGQTEVEISNIADITVVVFNPNTGDSIQTIKAGLTEIGDIYLINKSDLAGTNQLFDAVRDFIGDSVRNPIILKTSVKKNSGITDFAKTLKEMMSTKKKFKKEKQQEQLESELTDIVLNNIKEKIDHMLESDKSFSKYLKQLQSKSIDPFDAGDKITKSLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSC0</accession>
    <name>A0A2S2KSC0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11460</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876987.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSC0"/>
    <dbReference type="GeneID" id="76208510"/>
    <dbReference type="OrthoDB" id="10573at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="93" mass="10767" checksum="FADD27C268735958" modified="2018-07-18" version="1">MKISITFDDKYEVQKFGSLIFIKEGKETYITSILNIIKNELVILLKDKSAHSIMFKDEESVEKFADFIQSVLEKKHKIISTKIDGLTIEIVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSD4</accession>
    <name>A0A2S2KSD4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aminopeptidase</fullName>
            <ecNumber evidence="11">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">NZNM25_13630</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9 11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the peptidase M1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="11"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877177.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSD4"/>
    <dbReference type="GeneID" id="76209137"/>
    <dbReference type="OrthoDB" id="139771at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000002">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000006">
        <property type="entry name" value="Puromycin-sensitive aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF174">
        <property type="entry name" value="PUROMYCIN-SENSITIVE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11 15" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 11" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="14">
        <location>
            <begin position="5"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="12">
        <location>
            <begin position="217"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="13">
        <location>
            <begin position="506"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="290"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GBH34572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="833" mass="95466" checksum="B8A7CE9E5BA344B0" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSE0</accession>
    <name>A0A2S2KSE0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_12780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829081.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSE0"/>
    <dbReference type="GeneID" id="76209223"/>
    <dbReference type="OrthoDB" id="382717at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="57" mass="6328" checksum="0572BDA821518565" modified="2018-07-18" version="1">MAKNLIISTLVIIIAVLVGFIIMTNHDWFSQEWLIGLSVIVGGVIAEGIMLYLKIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSI1</accession>
    <name>A0A2S2KSI1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Aspartate aminotransferase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hemL</name>
        <name evidence="7" type="ORF">NZNM25_14130</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-4-amino-5-oxopentanoate = 5-aminolevulinate</text>
            <dbReference type="Rhea" id="RHEA:14265"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="5.4.3.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Porphyrin-containing compound metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829087.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSI1"/>
    <dbReference type="GeneID" id="76209082"/>
    <dbReference type="OrthoDB" id="6524at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042286">
        <property type="term" value="F:glutamate-1-semialdehyde 2,1-aminomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43713">
        <property type="entry name" value="GLUTAMATE-1-SEMIALDEHYDE 2,1-AMINOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43713:SF3">
        <property type="entry name" value="GLUTAMATE-1-SEMIALDEHYDE 2,1-AMINOMUTASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="435" mass="48129" checksum="AD66A3733E806862" modified="2018-07-18" version="1">MNLVLDYTREYKKKTPQSAKLFAKSAKLHVNGVSHNIRFYEPYPFVVKSSTGKNLVDVDSNKYTDYWMGHWSLILGHRQKNVKDALKKQIEKSWMYGTVNMQTILLSELISKAVPVAEKIRYVTSGTEATMYAVRLARSVTGKKIIAKIDGGWHGYTSDLLKSVNWPFTESESSGVVNEEKIVSIPYNDLEASLFILKKHSNDLAGVIIEPVLGGGGCIPAESDYLKGIQEFCKKNNSLFILDEIVTGFRFRYGCIYPTMKLDPDIVTLGKITGGGMAIGVMCGKKEIMEYADTTGKKKSERSYVGGGTFSANPTSMTAGFATLSILKNNKSIYSTINSIGDYARKELTKAFDGKGVVTGKGSLFMTHFVNNGTVEITNAAQAAKCDTRALHDYHFKMIAHDGIFFLPGKLGAISYTHTKQDIKKMVLATSDYLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSJ1</accession>
    <name>A0A2S2KSJ1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Poly(3-hydroxyalkanoate) polymerase subunit PhaE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_14000</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Biopolymer metabolism; poly-(R)-3-hydroxybutanoate biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSJ1"/>
    <dbReference type="OrthoDB" id="8049at2157"/>
    <dbReference type="UniPathway" id="UPA00917"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042619">
        <property type="term" value="P:poly-hydroxybutyrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010123">
        <property type="entry name" value="PHA_synth_III_E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09712">
        <property type="entry name" value="PHA_synth_III_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0583">PHB biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="168"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="199" mass="23317" checksum="097B079D570C0E97" modified="2018-07-18" version="1">MQPEQTKDKAEISKEMSDQFKHLSLFWTDIMHLMASKPQALTSIGPMRAFASNSKKISTELIEINEDLMEFNKYLTEYYKQLSEAWGVAQKKVNLKAPEVPQDIEQIEAFKRIWIDIFDNDFTELFDSAKFGENYGKLVSKELELTKHWNNISNVILQSVNLPSKEEIDEVYKEIHSLKKRVGRLELELKKREMKKNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSK0</accession>
    <name>A0A2S2KSK0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aminotransferase</fullName>
            <ecNumber evidence="7">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_12260</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="7"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877073.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSK0"/>
    <dbReference type="OrthoDB" id="372018at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050596">
        <property type="entry name" value="AspAT/PAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383:SF1">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="8">
        <location>
            <begin position="67"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="452" mass="51026" checksum="A65186CAFEBC9D5D" modified="2018-07-18" version="1">MKFVVDQQVKDIEMPENLKLNTFLQEFHSDCPHPECSFGFYGFAFGQSPFPVPKLIQDALVKNACKGAYAPISGIPELRDAISKYNKHYFGMDVSTERIHVGPGTKELIFNLLEVLHGTVILPTPAWLGYLPQIRYLKKNYHMLPTRSNKKISPTSLKKLALRLHDRQKILILNNPNNPTGLLYNKLELEEISDVCREQNITVISDEIYAQTTYDFSKFVSMGKIYPEGTFVTNGLSKSHAAGGYRLGYVIFPQDTADLSTQFKKILATEYTAVSTPIQHAAVAGFEISKEMEEYFEVTRSIHQIMGEYTYDGLSSIKGVTATKPDATFYLLADFNYYAADLEKSKILTSQKLSESLIIHPYHTAIVGGDSLVLERTDFSARIAYVDYDGSKVYQNYLDNKPKSNSEKTAFVEHNAPKVVAGIEMIKTFFDNIQTTFVRDLQSQKNSMMTPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSM4</accession>
    <name>A0A2S2KSM4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">3-hydroxyacyl-CoA dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_14340</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSM4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000009">
        <property type="entry name" value="Fatty oxidation complex, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075">
        <property type="entry name" value="3-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075:SF5">
        <property type="entry name" value="3-HYDROXYBUTYRYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase NAD binding" evidence="3">
        <location>
            <begin position="36"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="2">
        <location>
            <begin position="219"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="2">
        <location>
            <begin position="337"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="412" mass="46343" checksum="ABE915C7C43254E5" modified="2018-07-18" version="1">MRINFDSKNFELQNRTTIVKYIIKDLNFLESVSIKNITVLGSGVMGHGIAQVSATAGYNVVLRDIKQEFLDKAMEKIRWSLDKLVSKEKISKDEGDSIFARIKPIVDLAEAVKDAELVIEVVPEIMDLKKTVYAELDKVAAPEVIFASNTSTLPITEIANTTSRPDKFIGIHFFNPPQLMKLVEIIPGEKTSQKITDLTQEYVKSVNKIAVLCRKDVPGFIINRLFIPMVHEACFVKDRTGATLEEIDSAVKFKLRFPMGIFELADFTGMDVIHKATVEMHLRDKKVINPHHLVEKMFDEKKLGQKSGEGFYKYSDDKYERVALSEELAQKCNPIQLVANIINNAAWLVTNGASDIEEIEKAAQLGLGLKKPLFETAKEIGIKNIVDELNKLATEHGEFYKPDPLLVSMQTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSV7</accession>
    <name>A0A2S2KSV7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Exosome complex component Csl4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">csl4</name>
        <name evidence="4" type="ORF">NZNM25_15610</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Non-catalytic component of the exosome, which is a complex involved in RNA degradation. Increases the RNA binding and the efficiency of RNA degradation. Helpful for the interaction of the exosome with A-poor RNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the archaeal exosome complex. Forms a trimer of Rrp4 and/or Csl4 subunits. The trimer associates with an hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers. Interacts with DnaG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSL4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877391.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSV7"/>
    <dbReference type="GeneID" id="76208426"/>
    <dbReference type="OrthoDB" id="6768at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00975">
        <property type="entry name" value="Exosome_Csl4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039771">
        <property type="entry name" value="Csl4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025721">
        <property type="entry name" value="Exosome_cplx_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030850">
        <property type="entry name" value="Exosome_Csl4_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF034126">
        <property type="entry name" value="PRK09521.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12686">
        <property type="entry name" value="3'-5' EXORIBONUCLEASE CSL4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12686:SF8">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT CSL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14382">
        <property type="entry name" value="ECR1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110324">
        <property type="entry name" value="Ribosomal L27 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0271">Exosome</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Exosome complex component N-terminal" evidence="3">
        <location>
            <begin position="7"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="183" mass="20013" checksum="0AADAD06FB1319E8" modified="2018-07-18" version="1">MSENATFPGDKIASIEEYEAGNNTFDDGDMVRAATIGEKDIDKTTRIASIKHPKLLSIPQVDDIVIGTVAAVMSSMIAVSIDYINGRPTTSKVECVCSTRNLRIRNVALVNDIVALKILNHLNGTIHATISEPNLGVLFTKCRKCGGKVVPMRDAIKCTECAWIDERKLSSNFGNSDFIKLRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSZ8</accession>
    <name>A0A2S2KSZ8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glucose sorbosone dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_15550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877387.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSZ8"/>
    <dbReference type="GeneID" id="76208433"/>
    <dbReference type="OrthoDB" id="6744at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012938">
        <property type="entry name" value="Glc/Sorbosone_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH_b-prop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19328">
        <property type="entry name" value="HEDGEHOG-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19328:SF13">
        <property type="entry name" value="HIPL1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07995">
        <property type="entry name" value="GSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="Soluble quinoprotein glucose dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose/Sorbosone dehydrogenase" evidence="1">
        <location>
            <begin position="49"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="363" mass="40108" checksum="0802F74FA30DBBE3" modified="2018-07-18" version="1">MDKKIQIVAIVGAIIFSVLVLTSPSDPIPLPKPISNSQNDFVAILAEKLDKPRAIAVYENRIFVTEKDGLIRVVQDGNLLESPLAAFRPANVFDGGLLGIALHPNFPNNHFLYVFLTYEEDGELWNKIIRITESENKLQDAETILDKIPGSSFTNGGFIKFGPDEKLYVGTGTISDASHLPQDLNSLSGKILRLNDDGTIPDDNPFSDSPVYSLGHRNPQGMTWDDKGNLYVSEFGPEKNDEINMIKAGKNYGWPEQECSGNVNFEDAVLCYDPSIEPGGILFYTGDKLDFEFPFIMASMRSANLYQVDFEEGLSSQKSILSGIGRVRDVVEGDDGSLYVITSNTDGKGFPDRTDDKLLRILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT17</accession>
    <name>A0A2S2KT17_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 19 20">Adenosylcobinamide-GDP ribazoletransferase</fullName>
            <ecNumber evidence="5 19 20">2.7.8.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16 19">Cobalamin synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15 19">Cobalamin-5'-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">cobS</name>
        <name evidence="21" type="ORF">NZNM25_15770</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14 19">Joins adenosylcobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin). Also synthesizes adenosylcobalamin 5'-phosphate from adenosylcobinamide-GDP and alpha-ribazole 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 19">
            <text>alpha-ribazole + adenosylcob(III)inamide-GDP = adenosylcob(III)alamin + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16049"/>
            <dbReference type="ChEBI" id="CHEBI:10329"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="EC" id="2.7.8.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 19">
            <text>alpha-ribazole 5'-phosphate + adenosylcob(III)inamide-GDP = adenosylcob(III)alamin 5'-phosphate + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23560"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57918"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="ChEBI" id="CHEBI:60493"/>
            <dbReference type="EC" id="2.7.8.26"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 19">Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 7/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 19">Cell membrane</location>
            <topology evidence="2 19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the CobS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.26" evidence="5 19 20"/>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877422.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT17"/>
    <dbReference type="GeneID" id="76208410"/>
    <dbReference type="OrthoDB" id="11748at2157"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00238"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051073">
        <property type="term" value="F:adenosylcobinamide-GDP ribazoletransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008818">
        <property type="term" value="F:cobalamin 5'-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00719">
        <property type="entry name" value="CobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003805">
        <property type="entry name" value="CobS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00317">
        <property type="entry name" value="cobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34148">
        <property type="entry name" value="ADENOSYLCOBINAMIDE-GDP RIBAZOLETRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34148:SF1">
        <property type="entry name" value="ADENOSYLCOBINAMIDE-GDP RIBAZOLETRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02654">
        <property type="entry name" value="CobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 19" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 19" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="11 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 19 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="176"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="NCBIfam" id="TIGR00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="GBH34786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="241" mass="25350" checksum="D7DE78296D96C9F2" modified="2018-07-18" version="1">MLKEISSVFSFLTIFPSSNTTLENIAKYMYLFPIVGIAIGLLVGSFGFGLSFFLDPLLVSLLVVAFIAIVTGIHHADGLADFADGLMVKGSKDKKLKAMKDLSTGSAGIVGIVLYLIGLVITVSLTSGFDLFKAILISEILAKFSMVLLASLGNSASLGSNSPFVDVMKDKKKLTAAFIIMLIPVIAIGETTGLVMLGVTVTLVIFLLALSTRSFGGITGDVLGATNELTRLASLMVFVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT21</accession>
    <name>A0A2S2KT21_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Roadblock/LAMTOR2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13990</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877211.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT21"/>
    <dbReference type="GeneID" id="76209100"/>
    <dbReference type="OrthoDB" id="1734at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046600">
        <property type="entry name" value="DUF6659"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20364">
        <property type="entry name" value="DUF6659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="130" mass="15155" checksum="131B5F9C96CD8C57" modified="2018-07-18" version="1">MDNAEELEKICQKILKLDPMMRSARIINSRGHLVAGGMKKGIFSLEAQKQDEMMFMELALRVRMRREFDHEFGEVHFSMSYREKVIVMSFPLNNDDVLLLSCEKNTDFGKLPFKVLKIIKPLKKSPTKTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT50</accession>
    <name>A0A2S2KT50_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_16150</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT50"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="71" mass="8444" checksum="8B005FBD59EAA60B" modified="2018-07-18" version="1">MIDPTFGTFHIVLYCKIHKISMIKMSKRVTIMIDEDLDKKLRLRQAKLIQQEQTSYSYSKVLNESLRKVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT58</accession>
    <name>A0A2S2KT58_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Vitamin B12-dependent ribonucleotide reductase</fullName>
            <ecNumber evidence="12">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">NZNM25_16250</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + [thioredoxin]-disulfide + H2O = a ribonucleoside 5'-diphosphate + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ribonucleoside diphosphate reductase class-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="12"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877461.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT58"/>
    <dbReference type="GeneID" id="76208360"/>
    <dbReference type="OrthoDB" id="6188at2157"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02888">
        <property type="entry name" value="RNR_II_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.70.20:FF:000022">
        <property type="entry name" value="Vitamin B12-dependent ribonucleotide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050862">
        <property type="entry name" value="RdRp_reductase_class-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013509">
        <property type="entry name" value="RNR_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013344">
        <property type="entry name" value="RNR_NrdJ/NrdZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008926">
        <property type="entry name" value="RNR_R1-su_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02504">
        <property type="entry name" value="NrdJ_Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43371:SF1">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43371">
        <property type="entry name" value="VITAMIN B12-DEPENDENT RIBONUCLEOTIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00317">
        <property type="entry name" value="Ribonuc_red_lgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48168">
        <property type="entry name" value="R1 subunit of ribonucleotide reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 12" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="9 12" id="KW-0170">Cobalt</keyword>
    <keyword evidence="7" id="KW-0215">Deoxyribonucleotide synthesis</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="4 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-cone" evidence="13">
        <location>
            <begin position="11"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GBH34834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="883" mass="99450" checksum="751A17D37054242B" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT74</accession>
    <name>A0A2S2KT74_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_16270</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829102.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT74"/>
    <dbReference type="GeneID" id="76208358"/>
    <dbReference type="OrthoDB" id="3973at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="53" mass="5767" checksum="E87827666DEE41C4" modified="2018-07-18" version="1">MSVEITCGKCGEKITNMKMLKSLKDVLNPTNGKCPSCGQKLSTSEFSLDVQEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT76</accession>
    <name>A0A2S2KT76_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_14560</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT76"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="175" mass="19690" checksum="D261B43FBD5F4544" modified="2018-07-18" version="1">MIKTMAIILAAIMLASTGFIVSADAGTIVPGHEKQSKKDKDNNGIPDKGVTVNGHYTSVYAYDNLGDWYWDLGDGRIQGTVDSVEELDSSTLTVCDYVVNFRGNFENDPFMDSGWIQNHINCHGYDDNGHYNYLIVHETDPRYRDNPEWSIWGTWEYHVLTESGIGNLVRPMVPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT81</accession>
    <name>A0A2S2KT81_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_14590</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the PINc/VapC protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877352.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT81"/>
    <dbReference type="GeneID" id="76209036"/>
    <dbReference type="OrthoDB" id="7146at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00105">
        <property type="entry name" value="KH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09878">
        <property type="entry name" value="PIN_VapC_VirB11L-ATPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052041">
        <property type="entry name" value="Nucleic_acid_metab_PIN/TRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010335">
        <property type="entry name" value="PRK13764.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11603">
        <property type="entry name" value="AAA FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11603:SF147">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13638">
        <property type="entry name" value="PIN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PIN" evidence="5">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="259"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="481"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00046345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="605" mass="67458" checksum="0A03F840B85066CF" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT86</accession>
    <name>A0A2S2KT86_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Putative 3-methyladenine DNA glycosylase</fullName>
            <ecNumber evidence="5">3.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_14660</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the DNA glycosylase MPG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="5"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877265.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT86"/>
    <dbReference type="GeneID" id="76209029"/>
    <dbReference type="OrthoDB" id="31217at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003905">
        <property type="term" value="F:alkylbase DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00540">
        <property type="entry name" value="AAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.300.10:FF:000001">
        <property type="entry name" value="Putative 3-methyladenine DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.300.10">
        <property type="entry name" value="Methylpurine-DNA glycosylase (MPG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00527">
        <property type="entry name" value="3MGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003180">
        <property type="entry name" value="MPG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036995">
        <property type="entry name" value="MPG_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00567">
        <property type="entry name" value="3mg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002003">
        <property type="entry name" value="PRK00802.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10429">
        <property type="entry name" value="DNA-3-METHYLADENINE GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10429:SF0">
        <property type="entry name" value="DNA-3-METHYLADENINE GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02245">
        <property type="entry name" value="Pur_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="FMT C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4 5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="183" mass="20875" checksum="CDD4269ADDAE3075" modified="2018-07-18" version="1">MIPREFYSKDTITVARNILGKRIIRKIGRTELSGIITETEAYRHTDDPASHAFGRKTDRNKVMFEDVGHAYVYFTYGMHYCFNVVARHPKIKAGAVLIRAIKPEKGIEKMQKNRKIANTKDLTNGPAKLTQALEITKEHYGIDLTKDSRLHIAEGIKPKKIIASPRIGIKNATDKLWNFKIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT97</accession>
    <name>A0A2S2KT97_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Anhydromevalonate phosphate decarboxylase</fullName>
            <ecNumber evidence="6">4.1.1.126</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_15920</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the conversion of trans-anhydromevalonate 5-phosphate (tAHMP) into isopentenyl phosphate. Involved in the archaeal mevalonate (MVA) pathway, which provides fundamental precursors for isoprenoid biosynthesis, such as isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(2E)-3-methyl-5-phosphooxypent-2-enoate + H(+) = isopentenyl phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:78971"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:65078"/>
            <dbReference type="ChEBI" id="CHEBI:229665"/>
            <dbReference type="EC" id="4.1.1.126"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:78972"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>prenylated FMN</name>
            <dbReference type="ChEBI" id="CHEBI:87746"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UbiD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.126" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877430.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT97"/>
    <dbReference type="GeneID" id="76208394"/>
    <dbReference type="OrthoDB" id="8480at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1670.10:FF:000003">
        <property type="entry name" value="Phenolic acid decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1670.10">
        <property type="entry name" value="UbiD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002830">
        <property type="entry name" value="UbiD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049381">
        <property type="entry name" value="UbiD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049383">
        <property type="entry name" value="UbiD-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048304">
        <property type="entry name" value="UbiD_Rift_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00148">
        <property type="entry name" value="UbiD family decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30108">
        <property type="entry name" value="3-OCTAPRENYL-4-HYDROXYBENZOATE CARBOXY-LYASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30108:SF21">
        <property type="entry name" value="4-HYDROXYBENZOATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01977">
        <property type="entry name" value="UbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20696">
        <property type="entry name" value="UbiD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20695">
        <property type="entry name" value="UbiD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143968">
        <property type="entry name" value="UbiD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like N-terminal" evidence="10">
        <location>
            <begin position="8"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like Rift-related" evidence="9">
        <location>
            <begin position="101"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like C-terminal" evidence="11">
        <location>
            <begin position="291"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF20696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH34801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="443" mass="49865" checksum="DE57E40091D22E94" modified="2018-07-18" version="1">MSDLRNYISQIKKINDLKIIKRKVSTKFEIAGITAKVDGSHAVLFENIKESNFHLVANLVGTRKRFALAMGGTENNIHAKIISAIKNAKHPRIIPSGQFLENKSKNLFSMPIVTHFEKESGPFITSSVAYAKNPETGKQNSSFHRMMPIDKTHFTIRMVEGRHLHRCFIDAKEHGEDLKIAITVGVHPAILIAGAYQAEWGQDEIDIANSLLGGKLTLTKLPFSGLNVPSGSEIVMEGKILRDKTHPEWMVEMLQTYDHKRSQPVFELENLYFRTNPIFHDVLSGYSEHRLLMGMPIESKLNGELKKAFPQTLQVSMTNGGCNWLHAVVQIKKKSDSDPKKIIKKTFASHRSLKQVTVVDEDIDPTNAESVEYAMATRFQADKDLMILKKVRGSSLDPSSNQKKLQTAKMGIDATRPLSKRPEGFELAKIPKINKINLEKYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTA7</accession>
    <name>A0A2S2KTA7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor E</fullName>
            <shortName evidence="4">TFE</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">TFIIE subunit alpha homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Transcription initiation factor TFIIE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tfe</name>
        <name evidence="6" type="ORF">NZNM25_16020</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Transcription factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Facilitates transcription initiation by enhancing TATA-box recognition by TATA-box-binding protein (Tbp), and transcription factor B (Tfb) and RNA polymerase recruitment. Not absolutely required for transcription in vitro, but particularly important in cases where Tbp or Tfb function is not optimal. It dynamically alters the nucleic acid-binding properties of RNA polymerases by stabilizing the initiation complex and destabilizing elongation complexes. Seems to translocate with the RNA polymerase following initiation and acts by binding to the non template strand of the transcription bubble in elongation complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer. Interaction with RNA polymerase subunits RpoF and RpoE is necessary for Tfe stimulatory transcription activity. Able to interact with Tbp and RNA polymerase in the absence of DNA promoter. Interacts both with the preinitiation and elongation complexes.</text>
    </comment>
    <comment type="domain">
        <text evidence="4">The winged helix domain is involved in binding to DNA in the preinitiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TFE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877440.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTA7"/>
    <dbReference type="GeneID" id="76208384"/>
    <dbReference type="OrthoDB" id="5935at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000606">
        <property type="entry name" value="Transcription factor E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01909">
        <property type="entry name" value="TFE_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016481">
        <property type="entry name" value="TF_E_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039997">
        <property type="entry name" value="TFE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017919">
        <property type="entry name" value="TFIIE/TFIIEa_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002853">
        <property type="entry name" value="TFIIE_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024550">
        <property type="entry name" value="TFIIEa/SarR/Rpc3_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097:SF7">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02002">
        <property type="entry name" value="TFIIE_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006373">
        <property type="entry name" value="TF_E_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00531">
        <property type="entry name" value="TFIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51344">
        <property type="entry name" value="HTH_TFE_IIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH TFE/IIEalpha-type" evidence="5">
        <location>
            <begin position="5"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="171" mass="20041" checksum="C49EE2B8299B0EBD" modified="2018-07-18" version="1">MVDKYEDPFVRIASMIGGDEYLKVARSLLKAEDATDEEIASSTGLRINMVRKVLYDLFGKSLITGIRVKDERKGWFVYRWRTRREEVEHFIESQKKKIEERLQQRLDYENASDFYHCGNEDCPRVTFESALDGMFKCPSCGSVLNLKKNDKSKKAYSKKIDEIKKDMQQTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTD2</accession>
    <name>A0A2S2KTD2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_17210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877535.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTD2"/>
    <dbReference type="GeneID" id="76208264"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="11"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="62" mass="7167" checksum="859DE117082FDEBD" modified="2018-07-18" version="1">MGLFGSSSNQLKCKKCGTVLSDSERLKKHNEKAHNKKKEKCRICGTEFDTQEELRKHKKNCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTH7</accession>
    <name>A0A2S2KTH7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Sodium:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_17450</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTH7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562">
        <property type="entry name" value="NAPA-TYPE SODIUM/HYDROGEN ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562:SF3">
        <property type="entry name" value="SODIUM ION_PROTON EXCHANGER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="173"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="247"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="285"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="315"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="352"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="378"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="11">
        <location>
            <begin position="24"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH34954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="411" mass="43278" checksum="5D1A3F1B98EB988E" modified="2018-07-18" version="1">MTMVTEFDAIPTLVGILVLVIPSMLLGKICKHFGISEIIGFVIGGIILSPFALGGLIQFSDRPIVELNELMLSFWQIAGILILFSAGLHFPFSSLKQAGIQSLIVGTAGVIVPLILGYGISVLLGIDWIVAMVIGATLSATSIAAAVTILDELGKEKTQEGNILVNAAVFDDVLGLAILSSIISIVIAHSLPSVELVLFEISESLILWVVLLLGSVFLLPKIVHAVATMRPNSLESRGTKQATALGSAFGVAAIAASIGLNPIVGAFAAGMGLADSKLSNQVKEFIGRLQIIFAPFFFAIIGTHVDISNILNVDLVLFVIILIIAVLGKILGSGIPAVILLKNKNKGLRIGYGMIVRGEIAFITAGLGLSYGIISESIFSTLIFVILGTIIIGPILLRRSFEKNDEISNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTM1</accession>
    <name>A0A2S2KTM1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADH:ubiquinone reductase (non-electrogenic)</fullName>
            <ecNumber evidence="2">1.6.5.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">NZNM25_17840</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a quinone + NADH + H(+) = a quinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:46160"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.9" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877589.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTM1"/>
    <dbReference type="GeneID" id="76210337"/>
    <dbReference type="OrthoDB" id="6639at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) (non-electrogenic) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054585">
        <property type="entry name" value="NDH2-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706:SF47">
        <property type="entry name" value="EXTERNAL NADH-UBIQUINONE OXIDOREDUCTASE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22366">
        <property type="entry name" value="NDH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="9">
        <location>
            <begin position="7"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="External alternative NADH-ubiquinone oxidoreductase-like C-terminal" evidence="10">
        <location>
            <begin position="367"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="451" mass="51213" checksum="CB457982C77D673F" modified="2018-07-18" version="1">MARNKKKIVVLGGGFAGLECTLKLEKFFKNDLEIEIVLVSEDNFLLFTPMLPQVASGMIETRHIVMPIRTITKKAVFYEGRVKNIDPYGKIVNLWGSRDKRGISIHYDFLVVALGSETNFFGMSDLEKNAYQMKTLNDAVLVRNRIIDMLEQAENETNPILKKSFLTFVIVGGGFAGIETAGELMDFLLDVRKYYPNIKREDIRVIVLEALQNILPGFSESLAKFAQEKLSGRGIEIKLQTAVTSFNGDEVMIKRSDIDKDAIDDSMVSSIQSKTVIWTAGVTPVNTIKRSLFKTDKGKIIVDDNLEVVDFPGVFAIGDCALFLDPKTQRPFAPTAQIAEAQAKIAAKNLYSLIKNKEKTKFVYESKGQMAIIGKRTGIASFLGMNIHGIVAWLLWRNVYLSKIPTWDKRFRVFLDWTVDVFFDRDISRLKFMRREPEKEYKILDEVDDVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTP0</accession>
    <name>A0A2S2KTP0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ATPase AAA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_17870</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. CDC48 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877592.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTP0"/>
    <dbReference type="GeneID" id="76210334"/>
    <dbReference type="OrthoDB" id="77269at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000057">
        <property type="entry name" value="AAA family ATPase, CDC48 subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000018">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005938">
        <property type="entry name" value="AAA_ATPase_CDC48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01243">
        <property type="entry name" value="CDC48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="6">
        <location>
            <begin position="5"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="210"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="483"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="713" mass="78850" checksum="41055466D183AD82" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTS4</accession>
    <name>A0A2S2KTS4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable cobalt-precorrin-6B C(15)-methyltransferase (decarboxylating)</fullName>
            <ecNumber evidence="5">2.1.1.196</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cbiT</name>
        <name evidence="6" type="ORF">NZNM25_18340</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the methylation of C-15 in cobalt-precorrin-6B followed by the decarboxylation of C-12 to form cobalt-precorrin-7.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Co-precorrin-6B + S-adenosyl-L-methionine = Co-precorrin-7 + S-adenosyl-L-homocysteine + CO2</text>
            <dbReference type="Rhea" id="RHEA:36067"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:70791"/>
            <dbReference type="ChEBI" id="CHEBI:72780"/>
            <dbReference type="EC" id="2.1.1.196"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 8/10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the methyltransferase superfamily. Archaeal-type CbiT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.196" evidence="5"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877633.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTS4"/>
    <dbReference type="GeneID" id="76210287"/>
    <dbReference type="OrthoDB" id="6027at2157"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00229"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043776">
        <property type="term" value="F:cobalt-precorrin-6B C5-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019251">
        <property type="term" value="P:anaerobic cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00786">
        <property type="entry name" value="CbiT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023475">
        <property type="entry name" value="CbiT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014008">
        <property type="entry name" value="Cbl_synth_MTase_CbiT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050714">
        <property type="entry name" value="Cobalamin_biosynth_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02469">
        <property type="entry name" value="CbiT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43182">
        <property type="entry name" value="COBALT-PRECORRIN-6B C(15)-METHYLTRANSFERASE (DECARBOXYLATING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43182:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-7 C(5)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01135">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="2 5 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5 6" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="47"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH35043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="196" mass="21598" checksum="EAE591ED492B428D" modified="2018-07-18" version="1">MWNYKTPGIPDEYFERTENVPITKEEVRTIQISKARLKPGQTIYDIGCGSGSISVEAGFQIESTGKVLAIDHDENAIELTKKNMKKFGLSNISVIFGDAKEKIKDLEEADVIFIGGTGGDTRDIVELSENKLKSGGRIVIGIILIETLYSVLQILDKLQFESIDITQVTISKSRKTSTGTMMLARNPVTIISATRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTT7</accession>
    <name>A0A2S2KTT7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_18550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877679.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTT7"/>
    <dbReference type="GeneID" id="76210082"/>
    <dbReference type="OrthoDB" id="4958at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="317" mass="35967" checksum="E0F3518585CEB061" modified="2018-07-18" version="1">MQEIVLILSSLAGVATAAAVRKMPRDKNQLLSLGASSHIKSQINSLKIEKDILTKTISRLYQADTEFSKIQKDKLLLKYQHQLGIVLAKLEKLEQASKHPDLGPVGDGLITLMDQKLSKLDDRLYELSSKIISSKAETESTVKESKQDISKSFKNTFNFEVPKKTETKPIEIPATKSRQSFELTTLTNITRKEPKFPLFEKEEKQVVKPMPQPRVMQTELIKPKEEIVEEIIQPKPKVEDKIEIIQSVTPKPEVNFETKPDINKEVAKITEHKALPEPEKPKTTTLDDDFDDDTDDLDKIKGNIMRVLSKLDQAEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTU9</accession>
    <name>A0A2S2KTU9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA polymerase II small subunit</fullName>
            <shortName evidence="15">Pol II</shortName>
            <ecNumber evidence="15">2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Exodeoxyribonuclease small subunit</fullName>
            <ecNumber evidence="15">3.1.11.1</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">polB</name>
        <name evidence="17" type="ORF">NZNM25_17780</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 15">Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3' to 5' direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 15">Heterodimer of a large subunit and a small subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the DNA polymerase delta/II small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="15"/>
    <dbReference type="EC" id="3.1.11.1" evidence="15"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877583.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTU9"/>
    <dbReference type="GeneID" id="76208207"/>
    <dbReference type="OrthoDB" id="372039at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042575">
        <property type="term" value="C:DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.50:FF:000003">
        <property type="entry name" value="DNA polymerase II small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00325">
        <property type="entry name" value="DNApol_II_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024826">
        <property type="entry name" value="DNA_pol_delta/II_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011149">
        <property type="entry name" value="Pol2_small_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003118">
        <property type="entry name" value="PRK04036.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416:SF0">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000803">
        <property type="entry name" value="Arc_Pol2_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0235">DNA replication</keyword>
    <keyword evidence="11 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 15" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="9 15" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7 15" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="16">
        <location>
            <begin position="219"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GBH34987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="481" mass="54196" checksum="92FD5A4E8DF46ECF" modified="2018-07-18" version="1">MKKELSFVLNYALNRGFQIHPDAFKILENLDVKKLEKIIKEIVREKTRQKLFQINQDDLETYLGIKEDLTLQSEVKILSEPTSKITSGEGVKGYNALFSSRFNKLKRIISDRPESKMLKSAASVKTAKSDDDIYVCGLVSTRNSERNITKLILEDPSGSFEGIIFDNELQKTANALLMDQFVMARISLGKNSGYIIKDLILPDIPDQAKNKSESEAYAVFLSDLHIGSKYFMEDEFSEFVSWLSSPDPVARKIRFVLIGGDVVDGVGIYPNQDKELVCQTIQKQLKKVEELIDKIPKNIKIIIMPGNHDPGRRALPQPAIPKKYNSGLWERENVVMVGNPAFVSLNGVKIMMFHGQSIDDIVKTTPGLSYDKPTNVMRHLLRARHLSPIYGSQTPIAPEMEDLLVIEDIPDIFHVGHVHRAELDMYKGILLVNSGSWQKQTPFQASVGMTPNPGIAIMVNLKTFQVFHENYSSNSDNVLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTY9</accession>
    <name>A0A2S2KTY9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_19030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877721.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTY9"/>
    <dbReference type="GeneID" id="76210130"/>
    <dbReference type="OrthoDB" id="374997at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="65" mass="7642" checksum="24640B1B1DA2E3F9" modified="2018-07-18" version="1">MEDEVDFEKCADPLMIILNSLELIKLQYDGVMDDGIKAYLNRIDKSAQKIERLLQELKTKHKIYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTZ7</accession>
    <name>A0A2S2KTZ7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histidine phosphatase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_19150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877729.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTZ7"/>
    <dbReference type="GeneID" id="76210144"/>
    <dbReference type="OrthoDB" id="304253at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050275">
        <property type="entry name" value="PGM_Phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100">
        <property type="entry name" value="BROAD-SPECIFICITY PHOSPHATASE YOR283W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100:SF1">
        <property type="entry name" value="HISTIDINE PHOSPHATASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="8"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="207" mass="23260" checksum="4958DBFF223A510F" modified="2018-07-18" version="1">MGHIIFLRHGQAKNNVERILAGRTEGVPLTDVGIKQAEHTAELLKHMNVSAIYSSPIERAKHTAEIVGGHNSLDVTIDDRLIELDMGKFTGVPYDEIFTSHGNVFMKFYNGELEIAHNGVETFAEVKKRVLGIVDHVIEKHPDENVVLVTHMDPIKAMLSTIVDLSPTNLFELIIENASLNLFREKDRKFSLSGLNVMHPSRFDLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU39</accession>
    <name>A0A2S2KU39_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Serine O-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_19540</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877764.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU39"/>
    <dbReference type="GeneID" id="76210184"/>
    <dbReference type="OrthoDB" id="1475at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04647">
        <property type="entry name" value="LbH_MAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051159">
        <property type="entry name" value="Hexapeptide_acetyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416:SF23">
        <property type="entry name" value="ACETYLTRANSFERASE C18B11.09C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416">
        <property type="entry name" value="SIALIC ACID SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14602">
        <property type="entry name" value="Hexapep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="176" mass="19575" checksum="A841D8B841511B39" modified="2018-07-18" version="1">MINSKNFRDNEQELMDYYGYVGFGGRIKIRIKFLKSWIFHSLAYSSPLPSWSIKFQRMRGVKIGENCHISPYVLIDLLHPELIKIEDNVTISSNSMIFAHVNPSANDFLKKHGYPRTIKSVIVKKGAVISVGCIIIAGVTIGENAIVGAGSVVTQDVPDYCVVVGNPARVVKKIDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU59</accession>
    <name>A0A2S2KU59_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_19600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146196015.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU59"/>
    <dbReference type="GeneID" id="76210190"/>
    <dbReference type="OrthoDB" id="57427at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="106"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="308" mass="36524" checksum="917604472D475BA0" modified="2018-07-18" version="1">MNKNYEEKLLKFLKLFWLRPENGLLLAFKSGIMEDIKFESPSLDLSCGDGMYMFTHLGGTFSDNFDYFKSTQAEKFSHDSFVDIYDAFDPNYSPEIISPPKSQIDYGTDWKQTLLNKAATLNIYKNLILHDNNKKLPFEDNFFKTIYSNSIYWVETIDFLIADLYRILRPDGTLVLQIMTPYHHETLDKLDNLFSKEAIDILDRQRRDSYKGLKTYDEWIKLFKKAGFKIKETRNVYPNEFILDLWNIGLRPIAHLLIQMNDALSPEKRLQIKKPWVDIFFKLFKPLLFSQESFKFEKSPYLLFILEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUC0</accession>
    <name>A0A2S2KUC0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_20070</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="9">Belongs to the Lhr helicase family. Lhr-Core subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877816.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUC0"/>
    <dbReference type="GeneID" id="76210241"/>
    <dbReference type="OrthoDB" id="33870at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052511">
        <property type="entry name" value="ATP-dep_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017170">
        <property type="entry name" value="Lhr-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013701">
        <property type="entry name" value="Lhr-like_DEAD/DEAH_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045628">
        <property type="entry name" value="Lhr_WH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962:SF5">
        <property type="entry name" value="ATP-DEPENDENT HELICASE LHR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962">
        <property type="entry name" value="ATP-DEPENDENT HELICASE LHR-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08494">
        <property type="entry name" value="DEAD_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19306">
        <property type="entry name" value="WHD_Lhr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037307">
        <property type="entry name" value="Lhr-like_helic_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="34"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="239"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00093467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH35216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="913" mass="104037" checksum="5A65388E066FF57A" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUG7</accession>
    <name>A0A2S2KUG7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pyrroline-5-carboxylate reductase catalytic N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_19160</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUG7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070967">
        <property type="term" value="F:coenzyme F420 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008823">
        <property type="term" value="F:cupric reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000755">
        <property type="entry name" value="F420-dependent NADP reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010185">
        <property type="entry name" value="NpdG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051267">
        <property type="entry name" value="STEAP_metalloreductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01915">
        <property type="entry name" value="npdG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14239">
        <property type="entry name" value="DUDULIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14239:SF0">
        <property type="entry name" value="F420-DEPENDENT NADP REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="2">
        <location>
            <begin position="22"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="242" mass="26503" checksum="8C8556E1053FCCCD" modified="2018-07-18" version="1">MSQSERNSNKDNNKIVRRIIMKVGIIGGTGGMGKGFALRWSQNHDVIVGSRDAARAAESAVEYTNLAKEAFGEIKGSISGGDNVTVAKESDVLILSIPYENIDSVCSGILPEIRDSCVVVSPIVPMTKTDVGFECVSIKENKPFSYKLVLNHMKDKSKLVSAFHVISEKKLINPTLELDYDIFVCGDDESVKVVNTLIDEIKGLRSIYLGPIELSYLAEMSTPLLLNAMIRNKIKNPGIKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUK7</accession>
    <name>A0A2S2KUK7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">dTDP-glucose 4,6-dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_19560</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877766.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUK7"/>
    <dbReference type="GeneID" id="76210186"/>
    <dbReference type="OrthoDB" id="4907at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008460">
        <property type="term" value="F:dTDP-glucose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009225">
        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05246">
        <property type="entry name" value="dTDP_GD_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000304">
        <property type="entry name" value="UDP-glucose 4,6-dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005888">
        <property type="entry name" value="dTDP_Gluc_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01181">
        <property type="entry name" value="dTDP_gluc_dehyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="4">
        <location>
            <begin position="4"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH35165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="333" mass="38277" checksum="C542DA1D3622F0B9" modified="2018-07-18" version="1">MKILVCGGAGFIGSAFIRNHFNNHPKDEIINLDSLTIGSNLLNLKQIGQNQNYQFFKEDIKNQEIVNKLANDVDVIVNFAAESHVDRSIANPKPFIETNILGTYSLLEATKKYNKLFIHVSTDEVYGDAEDLDSFTEKSLIKPSNPYSATKASADHLVAAYHRTYNIKCITTRCTNNFGPFQFPEKLIPKTIIRAKKNLKVPLYGDGNQIRSWIYVYDHVQAIDDLILRGVHGQEYNITAWNEISNKIIVEKILKFLGKSDDLIEHVKDRPGHDRRYSIDAAKIQNEIGWKPKHEFEQALNETVEWYEQNQEWWEPLVDDNSLHSQPWTLTCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNN5</accession>
    <name>A0A2S2KNN5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00070</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875891.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNN5"/>
    <dbReference type="GeneID" id="76209333"/>
    <dbReference type="OrthoDB" id="12086at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="182" mass="20941" checksum="B1C7A882B5EBD6B3" modified="2018-07-18" version="1">MVEDGNPIKEYIFEYINNSQIIPKLISEKKFDQIIEDVTENCYDKIVSMGEKEEAVGILATGLLHYLLTNALITSQRKINYKGIEIDIVIPDIKTLEKDQKKTLLICILKSSDVNTINEKISLLEKIQHEKENIWLVLSKNIPIKKRSFILSKKNNSFSKIIFEIAQFSNVGGINKFKILRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNP5</accession>
    <name>A0A2S2KNP5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00540</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875934.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNP5"/>
    <dbReference type="GeneID" id="76209383"/>
    <dbReference type="OrthoDB" id="9852at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="155" mass="17961" checksum="3AD3090E24C9AFB5" modified="2018-07-18" version="1">MAKGRMRYWKITPEELGGYAYDENNLLNWEIKCVREPDDEAKFIGVFMYRHGTAYDYESVRGICYYHNNIDRKEIPSITNFLQGKFNGKEMEKGDRIFLKDSKEIFSAKDISELAKEMESKFNTKAIISLEFEGITAEKLKEAGLPEAKLLPIPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNP8</accession>
    <name>A0A2S2KNP8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876243.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNP8"/>
    <dbReference type="GeneID" id="76209346"/>
    <dbReference type="OrthoDB" id="4144at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18086">
        <property type="entry name" value="HsC9orf114-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003750">
        <property type="entry name" value="Put_MeTrfase-C9orf114-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150">
        <property type="entry name" value="CLASS IV SAM-BINDING METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150:SF13">
        <property type="entry name" value="METHYLTRANSFERASE C9ORF114-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02598">
        <property type="entry name" value="Methyltrn_RNA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="270" mass="30175" checksum="DFDD8EBFAF639B69" modified="2018-07-18" version="1">MKLSVAIPESALSDESLKIDKTRKISVLARACAIFKIDTIYVYQEGNNRTDGSLMVMILKYLETPQFLRRRLFPKINDLKFAGVLQPLKIPSHITPVNSKKINVGDIREGIVISVKGKRFVDVGINQLIPFFGKTSIGKRVTVQFKEGFPKLSVKEIGKSEVKTYWGYSVKERANLFSVLTEWKGNVIITSRKGKTATKEQISKYIKSEQPTLIVFGSPEKGIHEILGGKMKNVQNAKSLNFFPNQATETIRLEEALLGTLAIINAQSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNT6</accession>
    <name>A0A2S2KNT6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875998.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNT6"/>
    <dbReference type="GeneID" id="76209453"/>
    <dbReference type="OrthoDB" id="6843at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="103" mass="11860" checksum="BCE7365CCB141F8D" modified="2018-07-18" version="1">MSLEEIRKKVIFHNSVDVWIAACKEKNVPWTNPDNYKKFIAHLLKNNLNLKAFNLCAHEAGATEDEKTKFTETLAETKEDPNSLTYTIKLNDSALDIIRSYNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNX4</accession>
    <name>A0A2S2KNX4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01300</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876001.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNX4"/>
    <dbReference type="GeneID" id="76209459"/>
    <dbReference type="OrthoDB" id="4731at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="125" mass="14146" checksum="7586477DF7610742" modified="2018-07-18" version="1">MSRSRTITITVKKKTGDAFDAILQAPPKMMPDATINNDGWWLFTGPHGKSQLKFNENKSLGILDYQFIDEESTWNVPMRVVSNGDFSDVVITLNKPDELTDAQFDQRISEIGDMVVSMKNIIESY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNZ9</accession>
    <name>A0A2S2KNZ9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01530</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNZ9"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="81" mass="9681" checksum="CC27557074C95FB3" modified="2018-07-18" version="1">MARYPNVYCIQCGSNKKMVYDAVISTKNRHNPGMEIQVYWCMRCDIILRIQKSQVHNKINSIRVLMFKDRYAKDNKYGENF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP07</accession>
    <name>A0A2S2KP07_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zinc-ribbon domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_01050</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875979.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP07"/>
    <dbReference type="GeneID" id="76209434"/>
    <dbReference type="OrthoDB" id="3095at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="Zn_Ribbon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc-ribbon" evidence="2">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="233" mass="25747" checksum="26CEFD4B369B9482" modified="2018-07-18" version="1">MDSSDIVDDVNALLKLGVGDAYRLEHIKQGYIENKTIWVTDQNYLQRMREKYLIKQQPDKNADEGESIDADPKNKETIHCWKCGKKTPLEANFCMVCGSSLFEVGSNHNQKEKTADSMNQTKGKSLKLPIIIGIPVLILIVLGGAYSQGFFDNAFERYDTADSKKDDLTSKTTIPKEPFVNAVTDSKCGEGTVFDAETNSCVLDNGISPDRVNSKCGEGTVFDAETNSCVLDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP13</accession>
    <name>A0A2S2KP13_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoribosylformylglycinamidine synthase subunit PurQ</fullName>
            <shortName evidence="8">FGAM synthase</shortName>
            <ecNumber evidence="8">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Formylglycinamide ribonucleotide amidotransferase subunit I</fullName>
            <shortName evidence="8">FGAR amidotransferase I</shortName>
            <shortName evidence="8">FGAR-AT I</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Glutaminase PurQ</fullName>
            <ecNumber evidence="8">3.5.1.2</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Phosphoribosylformylglycinamidine synthase subunit I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">purQ</name>
        <name evidence="9" type="ORF">NZNM25_00520</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="8"/>
    <dbReference type="EC" id="3.5.1.2" evidence="8"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875932.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP13"/>
    <dbReference type="GeneID" id="76209381"/>
    <dbReference type="OrthoDB" id="6486at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01740">
        <property type="entry name" value="GATase1_FGAR_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00421">
        <property type="entry name" value="PurQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010075">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurQ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01737">
        <property type="entry name" value="FGAM_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002957">
        <property type="entry name" value="PRK03619.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47552">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47552:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13507">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001586">
        <property type="entry name" value="FGAM_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01211">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="226" mass="25151" checksum="2C31C43FB431228E" modified="2018-07-18" version="1">MKVGVVVFPGSNCDRDMYHVLTDVFNLDAQYFWHEKPLPKNIDAVILPGGFSYGDRLRAGVIAAHSPIIKDVKKLAEKGIPILGVCNGFQILVESGLLPGVLLKNKSLNFMCEWTSLIVENNTTPFTNQFKLHQKIPIPIANGEGRYYADDDVLKQLKKKNQIVFRYSDVVNGSTDRIAGVCNEDENVVGMMPHPERAVEPEINPIDNKPSSLIFESLLVKMGVNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP18</accession>
    <name>A0A2S2KP18_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01870</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876055.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP18"/>
    <dbReference type="GeneID" id="76209518"/>
    <dbReference type="OrthoDB" id="12289at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="262" mass="30020" checksum="BFBF9D6B00155DBA" modified="2018-07-18" version="1">MKTIFFIIAVIVILFGFNNVYGLPEYEYNIGNLEWIDRCHLTGSSSYVIMVTDDDMNTNPGKIDRFDIEVWSDSENRVINVPVIETNNDAGIFEGTVFFRTTDDVLGHRVQAFTGDTVFAKYIDHTVLNDSGKTEIIATFTMSELSVLEWRGGIHPWKLVYDPCTMEHLEKNKDRLDQFNIVYPAPLKQIKSGLHSFEVKCKDSLELIFRYHDSSPACVKPESATKLIARTGWMENLTAFVYPTSTHVFHIVPCEYSIIYHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP55</accession>
    <name>A0A2S2KP55_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0033 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_02270</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfur carrier protein TusA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876093.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP55"/>
    <dbReference type="GeneID" id="76209561"/>
    <dbReference type="OrthoDB" id="45650at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00291">
        <property type="entry name" value="SirA_YedF_YeeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="entry name" value="TusA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33279">
        <property type="entry name" value="SULFUR CARRIER PROTEIN YEDF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33279:SF6">
        <property type="entry name" value="SULFUR CARRIER PROTEIN YEDF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SirA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01148">
        <property type="entry name" value="UPF0033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0033" evidence="2">
        <location>
            <begin position="9"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="78" mass="8719" checksum="52E4465B158154D5" modified="2018-07-18" version="1">MSESPAKKLDATGLFCPEPVFRTKIEIERMQVGETLTVSADDPAAEDDISRWVTRNGHELLDLSKDGDVITFQIKKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP76</accession>
    <name>A0A2S2KP76_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00860</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP76"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="48" mass="5749" checksum="DA6E2EB5A7302BAB" modified="2018-07-18" version="1">MSKCLYKNSQFETICTNETDLDSKLCIFHDLKYYQEHPDEISDAFQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPB8</accession>
    <name>A0A2S2KPB8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Sodium:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_03110</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876165.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPB8"/>
    <dbReference type="GeneID" id="76209648"/>
    <dbReference type="OrthoDB" id="12029at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562">
        <property type="entry name" value="NAPA-TYPE SODIUM/HYDROGEN ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562:SF3">
        <property type="entry name" value="SODIUM ION_PROTON EXCHANGER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="160"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="240"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="309"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="11">
        <location>
            <begin position="19"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH33520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="413" mass="43441" checksum="6C583F7B5CF6BE64" modified="2018-07-18" version="1">MVEAHLIETIIGIGILLFAAKLMAELFLRLKLPIVLGELLAGMIVGPFALGAFFVVDGKQLLQINDEIRILGEMGAIVILFMAGLEMTPKEFLKGGKASFTVGTLGVVVPFFAGLVVFQMFGFDALQSMLIATALTATSIAISIQVLSEFGKIKTPEARLIIGAAVVDDILAIAVLSVVSSIAGSDGGIDNIDISEIVITILQVLGFFAIMLIVAVVVIPKIITPRLWKAKGSVEGIATASFFGAAALAGSIGLSPIVGAFAVGMALSTTKVFEKVENYIGKIGLIFAPLFFAIIGAQVDLRAVDLNILILSGVVIVVAIVTKLFGCGLPAMMFLKSKRQGMRVGIGMISRGEVGLIVAGVGVTAGILTSEVYSTIIIMVAVTTIITPIWLKMEYRKEQKSGSAPSEQNIEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPD0</accession>
    <name>A0A2S2KPD0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Universal stress protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_02180</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876085.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPD0"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006276">
        <property type="entry name" value="UspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="143" mass="15996" checksum="3073045F87C63E36" modified="2018-07-18" version="1">MFQNILVPFDLSDQSTRAFKMALDVAKKYQSKVTLLTCIEGDAWHHKYYDSRADNELIKKQSKVAKKYVEKLESLAEKNNVVAKSVIITSKSVVNDIVTFAKTRKHDLIVIGSHGRTGFDKVLLGSVANGVSQKTRCPVLIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPD2</accession>
    <name>A0A2S2KPD2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_03340</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876182.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPD2"/>
    <dbReference type="GeneID" id="76209672"/>
    <dbReference type="OrthoDB" id="198277at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="21"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="127" mass="14896" checksum="0D07A020B12CEB58" modified="2018-07-18" version="1">MKRNDDEFKITLSTVNSIFDDVEHKILSPLSCLREFDNHWMLEFDLPLVNKKDIKVTFDGNYINVEAKLKEKYSEEKLGRITKFEYFKKSILLPGRIDSKKTIAKFQKGRLEIKISKKVVGNTIKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPD6</accession>
    <name>A0A2S2KPD6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875993.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPD6"/>
    <dbReference type="GeneID" id="76209448"/>
    <dbReference type="OrthoDB" id="1707at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="97" mass="11517" checksum="EB3B7748BF16FADE" modified="2018-07-18" version="1">MVKKKSFDPDKLVLTKHEILEFCDRVLKKWEKNPVKNRNNILAMSAVKTSVIWTDDESLKAIWSEIFAWIFELLYENAMSQDNVEWGNVMKKLKNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPG7</accession>
    <name>A0A2S2KPG7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_02580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876118.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPG7"/>
    <dbReference type="GeneID" id="76209593"/>
    <dbReference type="OrthoDB" id="11492at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="452" mass="48713" checksum="72859B3C2BA7C174" modified="2018-07-18" version="1">MSKAVPLTVLLAILVVVGTGSTAWAAQLDARINPNSDSSPFSMHYLKTVFIEYPNGGNLFDELQGKEWTVSGSADSSNPGVQELMAKLNQKLSNDGSHAKVSDLNVSYEFLLKGRAINTSIDYKVLLEGTLSDYVITKDAQRTLVDLGWRGMTVNDEVNIDGVEINIPINLLRDKEPEVYAVFAGTEADQVLHRPIINADFILEQPLTNWHFLFDPTGINVDAGTFGLDEEIAGFVVSSWTMGESSIREGRQVERVFTADVVADQKYSVRSVQSADQANLATIGFGALDVLDGLEIAGVTPKPPEGYATTSTGDFPIMIVYGMAGMAAIAGIAFFIVSNRSLKNEKVGQQGIDPTRLVGYQTSASSGGYQTNRGEAQLKDETDYQKTRSVYDNAQQQSPPPQASSTEEAACGCAASAEMGSECDCEMHGSCLCDASCKCNAEICKEQVRSMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPH9</accession>
    <name>A0A2S2KPH9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cold shock-like protein CspLB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cspLB</name>
        <name evidence="4" type="ORF">NZNM25_03710</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876217.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPH9"/>
    <dbReference type="GeneID" id="76209713"/>
    <dbReference type="OrthoDB" id="202422at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012156">
        <property type="entry name" value="Cold_shock_CspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050181">
        <property type="entry name" value="Cold_shock_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019844">
        <property type="entry name" value="CSD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11544">
        <property type="entry name" value="COLD SHOCK DOMAIN CONTAINING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002599">
        <property type="entry name" value="Cold_shock_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00352">
        <property type="entry name" value="CSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="3">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="63" mass="6991" checksum="0C93E631A809D01B" modified="2018-07-18" version="1">MEQGTVKWFNRTKGFGFIEREGGDDLFVHKSDVDGFINEGDKVEFEVGEGQKGPAAQKVKKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPL8</accession>
    <name>A0A2S2KPL8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03700</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876216.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPL8"/>
    <dbReference type="GeneID" id="76209711"/>
    <dbReference type="OrthoDB" id="8197at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="203" mass="23560" checksum="B296012259327A3D" modified="2018-07-18" version="1">MSKLALSYSGYVCAPYLHTHESVELKETWIKSKNIEKLFFVTGTFSTESKPYFSDSTNHYLLAKFKDSSHISKDLLQHNQDKTSFVFNIQDDLFQREVQGETNFVTIYYLEYGEDGEDFQEIANLLLKREKIEKAGFGNMNLFCLTPSKFTFPYSEHVVVIEVASEKSHQSVKKYCEQTRRDVNRKGMTMTNLLSLSILDQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPM1</accession>
    <name>A0A2S2KPM1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_02090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876077.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPM1"/>
    <dbReference type="GeneID" id="76209543"/>
    <dbReference type="OrthoDB" id="3317at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="493" mass="55491" checksum="C45E9D32B7E8A0CF" modified="2018-07-18" version="1">MKTKIILYEKILILIIGGSIALFGMFTTLPVYALEFEPLIAKNTDDTIVSTGIVGQPIIFETIITNNEEDADHFEIAFTTRTINDKILDHQKQSIPLKHGASTTVSYQFVPPLEGNYVSGIFYGEKPFSDIITFPALDDNKTYPKKTVRIYSDLSDDCLVACTDPSVMNVDVGTVVELSNITPDVRYISTGTYMEDKDGISWSSNNRFHSIIIPDRKSSFLFSQPGEYQLFLAEHRTNDVIGTIHVMSDQFREADKTFNILNKIMNDEDFGIPISSLHINPKNSVITVGIDDKHNPLFTLDVYKTMLYKQVGNVYLDIISNHDSFKTKLCDVNDAIAVRAMLDRDPVVQQFLQSYPSATFEHFKTPDEPGNPRTYSEFHHGMFLLRVLVLTYDQNDVCYPVYGYVIGYDDQSSEPKKGLFENMYPKSAGLSKPLSEIKKLSKPHNQIKSGISFDDVQCIDDLILVITSDGSSACVKPETKTKLIERGWAKNAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPN6</accession>
    <name>A0A2S2KPN6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8 13">Glutamyl-tRNA reductase</fullName>
            <shortName evidence="8">GluTR</shortName>
            <ecNumber evidence="3 8 13">1.2.1.70</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hemA</name>
        <name evidence="17" type="ORF">NZNM25_03900</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8 13">
            <text>(S)-4-amino-5-oxopentanoate + tRNA(Glu) + NADP(+) = L-glutamyl-tRNA(Glu) + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12344"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="EC" id="1.2.1.70"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8 13">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">Possesses an unusual extended V-shaped dimeric structure with each monomer consisting of three distinct domains arranged along a curved 'spinal' alpha-helix. The N-terminal catalytic domain specifically recognizes the glutamate moiety of the substrate. The second domain is the NADPH-binding domain, and the third C-terminal domain is responsible for dimerization.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 13">Belongs to the glutamyl-tRNA reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.70" evidence="3 8 13"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876235.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPN6"/>
    <dbReference type="GeneID" id="76209732"/>
    <dbReference type="OrthoDB" id="4562at2157"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00316"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008883">
        <property type="term" value="F:glutamyl-tRNA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019353">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process from glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05213">
        <property type="entry name" value="NAD_bind_Glutamyl_tRNA_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.30:FF:000001">
        <property type="entry name" value="Glutamyl-tRNA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000031">
        <property type="entry name" value="Glutamyl-tRNA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.30">
        <property type="entry name" value="Glutamyl-tRNA reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00087">
        <property type="entry name" value="Glu_tRNA_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000343">
        <property type="entry name" value="4pyrrol_synth_GluRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015896">
        <property type="entry name" value="4pyrrol_synth_GluRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015895">
        <property type="entry name" value="4pyrrol_synth_GluRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018214">
        <property type="entry name" value="GluRdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036453">
        <property type="entry name" value="GluRdtase_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036343">
        <property type="entry name" value="GluRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01035">
        <property type="entry name" value="hemA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43013">
        <property type="entry name" value="GLUTAMYL-TRNA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43013:SF1">
        <property type="entry name" value="GLUTAMYL-TRNA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00745">
        <property type="entry name" value="GlutR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05201">
        <property type="entry name" value="GlutR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000445">
        <property type="entry name" value="4pyrrol_synth_GluRdtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69742">
        <property type="entry name" value="Glutamyl tRNA-reductase catalytic, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69075">
        <property type="entry name" value="Glutamyl tRNA-reductase dimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00747">
        <property type="entry name" value="GLUTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 11" id="KW-0521">NADP</keyword>
    <keyword evidence="5 8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 8 13" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamyl-tRNA reductase N-terminal" evidence="16">
        <location>
            <begin position="10"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Quinate/shikimate 5-dehydrogenase/glutamyl-tRNA reductase" evidence="15">
        <location>
            <begin position="169"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrapyrrole biosynthesis glutamyl-tRNA reductase dimerisation" evidence="14">
        <location>
            <begin position="317"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8 9">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <begin position="49"/>
            <end position="52"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <begin position="112"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <begin position="187"/>
            <end position="192"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for activity" evidence="8 12">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF05201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GBH33599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="421" mass="47374" checksum="4C6FFC2469D11B79" modified="2018-07-18" version="1">MNQNIINARVTFRNSPIHILERFTIKNLESAYEQFKKHSGLDECVIIQTCNRIELFGKSKTYDIDKIKKTWATITGIEEEAFDENMEYVENKEALHHLLKLTSGLDSMVLGEEQILGQIKNSITSARETKASGQHLNTLFDKAIRIGTRIRNTSGIGAGGISVGSMAVKLAEENIDELKTKKVLLIGTGEVSTLVAKSLQRRGYTFDVTSRTLGRSEAFCETMGGNPIKFEEVLLGFYNYDVIFVATTAPYFLVTNERITEAMKNKKGGIMILDLSNPRTVDEKVATIGGIKLMNLDQIAEMVEKNMNARLNKVKTVENIINEEVSVLEASMKRLDAEPLVKDVFKNIENLREKEFQKALQMLDEKDEKKIKIIEELTKAVVESIVAIPMNNIRKASEQGKPDVLELASKLFDYKKQNQAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPQ0</accession>
    <name>A0A2S2KPQ0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146195969.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPQ0"/>
    <dbReference type="GeneID" id="76209639"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="72" mass="8171" checksum="106B8239D37AFA86" modified="2018-07-18" version="1">MTNEELNGISMRLDAILSVLMEQTVVQEKTAKDKISLLNDFDFDTHEIARILHTSNSNVAKELSLLKKRGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPQ2</accession>
    <name>A0A2S2KPQ2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Deoxyhypusine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_02390</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the deoxyhypusine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876101.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPQ2"/>
    <dbReference type="GeneID" id="76209573"/>
    <dbReference type="OrthoDB" id="17730at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034038">
        <property type="term" value="F:deoxyhypusine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.910.10:FF:000007">
        <property type="entry name" value="Probable deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.910.10">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002773">
        <property type="entry name" value="Deoxyhypusine_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036982">
        <property type="entry name" value="Deoxyhypusine_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002294">
        <property type="entry name" value="PRK01221.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703:SF0">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01916">
        <property type="entry name" value="DS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0386">Hypusine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="313" mass="35008" checksum="71A807B641FB061F" modified="2018-07-18" version="1">MIEPGRPVKDIEIDSDTSIENIFEELSQSGGFESVNLSDGLEILTEMISDKQCLRFVSFVGAVVSTGLRGIIKNMIKNKWFDVAITTCGALDHDIARHFSHYKEGSFTMDDMELSNQNIHRLGNVLVPMDSYGPLIEEKMQGFLEEEYQKGVKEMTTAEICKMIGKHLGEDSFLYWAHKNNVSVVVPGIMDGAVGSQIWLFAQKHSDFKLNMTGDADLLSGFIFKAEKSGAFMIGGGISKHHTLWWNQYREGLDYAFYITTAQEFDGSLSGALVREAISWGKVTQKARQSTLHAEVTTILPFIYAALIKKLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPW2</accession>
    <name>A0A2S2KPW2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SMC-Scp complex subunit ScpB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_03780</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876223.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPW2"/>
    <dbReference type="GeneID" id="76209720"/>
    <dbReference type="OrthoDB" id="8628at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051304">
        <property type="term" value="P:chromosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005234">
        <property type="entry name" value="ScpB_csome_segregation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34298">
        <property type="entry name" value="SEGREGATION AND CONDENSATION PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34298:SF2">
        <property type="entry name" value="SEGREGATION AND CONDENSATION PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04079">
        <property type="entry name" value="SMC_ScpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="21567" checksum="C976870B3938A46C" modified="2018-07-18" version="1">MTKIEDMNEATARIEAALYSSGRPLRVEDIIKASGTESRTKTLELLDTIMKKTKNTFKALEVVILPDGSYVMQLKPEYSSTVKRYASKPVLPNATLKTLSYIAYMQPISSKQLVETRGSGVYSHLKELRQLDYINHQNVGRLKIYSTTEKFQKYFGIQGDVEDLKQRLFSKVRKTASMSHANPTPQIVQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPY6</accession>
    <name>A0A2S2KPY6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_05000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876365.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPY6"/>
    <dbReference type="GeneID" id="76209852"/>
    <dbReference type="OrthoDB" id="386310at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="209" mass="23018" checksum="D6038114406D65E6" modified="2018-07-18" version="1">MINKKTITSHLAIITSLILLSSVLFGNNVSFGDTAQETTKAQITKQQAQLKAESEFSNSVAVAKSQYVDAKIAAKDNPEKLRQAKISYNKAIQIAKEILYQKLTLANEQYKQSITKNSPVKSTPTSPETKSGTSQAKLAYAKTIKDAKNSLDKNLTAIKSNYKKALDSAKNEFDENKAENNYKLATKKAMDEYNMAISKAKIALDKSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ10</accession>
    <name>A0A2S2KQ10_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">thioredoxin-dependent peroxiredoxin</fullName>
            <ecNumber evidence="2">1.11.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Thioredoxin peroxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">bcp</name>
        <name evidence="13" type="ORF">NZNM25_03390</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peroxiredoxin family. BCP/PrxQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876187.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ10"/>
    <dbReference type="GeneID" id="76209678"/>
    <dbReference type="OrthoDB" id="145578at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03017">
        <property type="entry name" value="PRX_BCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000326">
        <property type="entry name" value="Bacterioferritin comigratory protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050924">
        <property type="entry name" value="Peroxiredoxin_BCP/PrxQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006960">
        <property type="entry name" value="PRK09437.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801:SF4">
        <property type="entry name" value="AHPC_TSA FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801">
        <property type="entry name" value="THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="12">
        <location>
            <begin position="2"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="11">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH33548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="152" mass="17439" checksum="65EA646E8086E2EB" modified="2018-07-18" version="1">MISEGDTVPKFEISDANGKKIKSSDFKGKKHVIYFYPKDFTPGCTTEADEFSRDYKKFQKAGIEIIGISPDDVESHKKFCDKMSIKYPLLADVDKEVSKMFGVWDKKKFMGREYMGVIRSTFLVDEKGKIFKIYPKVKPAGHSKEVLDDFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ16</accession>
    <name>A0A2S2KQ16_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_05440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ16"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="106" mass="12514" checksum="A2A36148A3D611D5" modified="2018-07-18" version="1">MTKLVFCMDCGECLGEDRPNHAKHHLKKHPNHRKYLTIPIIDPLLLPNPDEWFRKHENRLSQRFYGQPKSNTEKTTDDETEPDLTPENQKFSLKSSDFGKRHRGFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ41</accession>
    <name>A0A2S2KQ41_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_05830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876436.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ41"/>
    <dbReference type="GeneID" id="76209935"/>
    <dbReference type="OrthoDB" id="55755at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="107" mass="12135" checksum="359C50639929FCE3" modified="2018-07-18" version="1">MHTEYEEFENIEDMFMYMASAAPPMKNTMPINSYKGYVMAFIPLSPATGETYLMIYTSGSLDPGIYEFDISSKSYKKVEGIERADKVYFICLTPKRNTLADAAINTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ64</accession>
    <name>A0A2S2KQ64_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chemotaxis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_04520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829034.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ64"/>
    <dbReference type="GeneID" id="76209804"/>
    <dbReference type="OrthoDB" id="11404at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="505" mass="56492" checksum="8BBCA628E7E6FC7A" modified="2018-07-18" version="1">MATKKKTSAQPKKVVKKVSKEPAPSAVLKKVASVSDSNKALQKEIKVMTKIFGENQKVLLSMKGMIDTLTSTLEHIQKQSKQINIIEEDTQKLYAGLNQVRTQSNLVNKINDQTAKLQDEINKISEIQKTTKPQELSQQVQDSMNSIKNNSQMIIKIAQRIDEVRDDLRKVSGKADSFLEISSEMNKLKNNLDEISDKTAKLDTGNQIIQSLKQEFERIADSAISTTDLNSELEAIKVTIDSISSKASKIDSLGGIIDGLKQQFETISSNAKSAENLSLQTIRDLGGKIDRIETEVGGLSKRADSTAFVGEGLKSVQEDISNFKQTVFDKTNSIEQKISSASDILKRQDASTVEFHKKSDKLFEEIQSIKSATNKSTSDSSKEMMALLKLSEYQSNVRMNTESKYGELKDIEKMAAQTADIVNLFDRISIESGEKIPLPQEVRQWAVSKILDCADKWEVRFSDVYSILTNSIGRDMLKETIRVQQVRDIYGIRAVDEIRKDLNIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ68</accession>
    <name>A0A2S2KQ68_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">precorrin-2 dehydrogenase</fullName>
            <ecNumber evidence="2">1.3.1.76</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_03890</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>precorrin-2 + NAD(+) = sirohydrochlorin + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15613"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58351"/>
            <dbReference type="ChEBI" id="CHEBI:58827"/>
            <dbReference type="EC" id="1.3.1.76"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.76" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876234.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ68"/>
    <dbReference type="GeneID" id="76209731"/>
    <dbReference type="OrthoDB" id="10510at2157"/>
    <dbReference type="UniPathway" id="UPA00262">
        <property type="reaction ID" value="UER00222"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043115">
        <property type="term" value="F:precorrin-2 dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.110">
        <property type="entry name" value="Siroheme synthase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028161">
        <property type="entry name" value="Met8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006367">
        <property type="entry name" value="Sirohaem_synthase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01470">
        <property type="entry name" value="cysG_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35330">
        <property type="entry name" value="SIROHEME BIOSYNTHESIS PROTEIN MET8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35330:SF1">
        <property type="entry name" value="SIROHEME BIOSYNTHESIS PROTEIN MET8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13241">
        <property type="entry name" value="NAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75615">
        <property type="entry name" value="Siroheme synthase middle domains-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="217" mass="24639" checksum="BB2CF8CE1AC08D16" modified="2018-07-18" version="1">MIVDLNIQNKRIIVIGGGNEAEKRVNSLLKQNCNILVISDTVNSKIRKLAKDNKIELKKQKIQDTKFISTLNPHMIITTTNDKKVNQKIVNEAKKKKIIVYSSDNPDESDFSNPAIIDFDKMVQIAIFTGGQSPAMSKKIKGKLEKALKNIITKEDISHIKIQKIVRKIAKESISAPTQRKECLHKIMSDNEIDQLIKDGQVKKAEKRAITILRNWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ79</accession>
    <name>A0A2S2KQ79_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">Mevalonate kinase</fullName>
            <shortName evidence="14">MK</shortName>
            <shortName evidence="14">MVK</shortName>
            <ecNumber evidence="3 14">2.7.1.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">mvk</name>
        <name evidence="17" type="ORF">NZNM25_06000</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the phosphorylation of (R)-mevalonate (MVA) to (R)-mevalonate 5-phosphate (MVAP). Functions in the mevalonate (MVA) pathway leading to isopentenyl diphosphate (IPP), a key precursor for the biosynthesis of isoprenoid compounds such as archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(R)-mevalonate + ATP = (R)-5-phosphomevalonate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17065"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13 14">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the GHMP kinase family. Mevalonate kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.36" evidence="3 14"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876450.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ79"/>
    <dbReference type="GeneID" id="76209953"/>
    <dbReference type="OrthoDB" id="19001at2157"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00098"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004496">
        <property type="term" value="F:mevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00217">
        <property type="entry name" value="Mevalonate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006205">
        <property type="entry name" value="Mev_gal_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022937">
        <property type="entry name" value="Mevalonate_kinase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00549">
        <property type="entry name" value="mevalon_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290:SF2">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 14" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="8 14 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11 14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="15">
        <location>
            <begin position="77"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="16">
        <location>
            <begin position="222"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GBH33809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="313" mass="34492" checksum="04D921D3338FD498" modified="2018-07-18" version="1">MKSKASAPGKVILFGEHFVVYGVKAILCAIDKRITVTAEKTKERKISIKSNIGKLELEPNKLISEINSPLKPFYYLADKIIKNYETGIKITVESDIPLGVGLGSSSACCVAGAAAISRLFSYATKEEILKLAIEAEKTIFENTSGADCTVCTYGGIMEYDKKNGFTKIKSKPNFHLVIANSNIEHSTETIVAGVREFKENNEEEFSRLCKEESKLIEDVLNELKTNNIIEIGKKVIQNQEYLEKIGISNNKLKEMVKIGHSESFGAKITGAGGGGCIFALTDDSNLNNTIKQFKNNKYDCFAVKIDFKGLDTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQA3</accession>
    <name>A0A2S2KQA3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NADH-quinone oxidoreductase subunit A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nuoA</name>
        <name evidence="8" type="ORF">NZNM25_06370</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I subunit 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876566.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQA3"/>
    <dbReference type="GeneID" id="56066762"/>
    <dbReference type="OrthoDB" id="9823at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030964">
        <property type="term" value="C:NADH dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1610">
        <property type="entry name" value="NADH:ubiquinone/plastoquinone oxidoreductase, chain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000440">
        <property type="entry name" value="NADH_UbQ/plastoQ_OxRdtase_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038430">
        <property type="entry name" value="NDAH_ubi_oxred_su3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11058">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11058:SF9">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00507">
        <property type="entry name" value="Oxidored_q4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="110" mass="12473" checksum="44BCFB34598B51AF" modified="2018-07-18" version="1">MFGFAVAAMAPALIISRMISPRKRSNPVKFLPMECGQVPTGEGRTHFMMQYYPYILMFVVFDVMAIFLYAWGSALLELPKSATLPMMGFLAIMFGAMAFALYQSGRRRIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQF3</accession>
    <name>A0A2S2KQF3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">4Fe-4S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_06840</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876522.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQF3"/>
    <dbReference type="GeneID" id="76210045"/>
    <dbReference type="OrthoDB" id="23833at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050572">
        <property type="entry name" value="Fe-S_Ferredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687">
        <property type="entry name" value="ADENYLYLSULFATE REDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687:SF6">
        <property type="entry name" value="L-ASPARTATE SEMIALDEHYDE SULFURTRANSFERASE IRON-SULFUR SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="67"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="127"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="182" mass="20151" checksum="ED34039DB142DF5C" modified="2018-07-18" version="1">MAELQIPEDFCHNDVKPTGKTSHADGENFHWIWGKGRTDGAAFSNEDVKAAYEARGEKQVPLGIHGTTVAVDWDSCVAAGACMSVCPVQTFQWYRTEKDIPADKCLGETFEGTGKTEQDERLDYTDKSQPIREHDCTVCMACQEICPEGAIRIESANQEWHEKAAGTYVLMKSGSENPHAHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQL1</accession>
    <name>A0A2S2KQL1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methylated-DNA-[protein]-cysteine S-methyltransferase DNA binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_05260</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:53428"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:13555"/>
            <dbReference type="Rhea" id="RHEA-COMP:13556"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:137386"/>
            <dbReference type="ChEBI" id="CHEBI:137387"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = S-methyl-L-cysteinyl-[protein] + a 2'-deoxyguanosine in DNA</text>
            <dbReference type="Rhea" id="RHEA:24000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:11367"/>
            <dbReference type="Rhea" id="RHEA-COMP:11368"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:85445"/>
            <dbReference type="ChEBI" id="CHEBI:85448"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876387.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQL1"/>
    <dbReference type="GeneID" id="76209877"/>
    <dbReference type="OrthoDB" id="372118at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003908">
        <property type="term" value="F:methylated-DNA-[protein]-cysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000787">
        <property type="entry name" value="Methylated-DNA--protein-cysteine methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001497">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00589">
        <property type="entry name" value="ogt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10815">
        <property type="entry name" value="METHYLATED-DNA--PROTEIN-CYSTEINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10815:SF13">
        <property type="entry name" value="METHYLATED-DNA--PROTEIN-CYSTEINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="Methylated DNA-protein cysteine methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00374">
        <property type="entry name" value="MGMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methylated-DNA-[protein]-cysteine S-methyltransferase DNA binding" evidence="7">
        <location>
            <begin position="3"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="99" mass="11134" checksum="0F99117ED8C09E15" modified="2018-07-18" version="1">MNLEQKIYKKLLEVPKGQITTYGELAKAVGLKNGQRVVGKIMNKNPYPVIIPCHRVVMSTGKIGGYAYGEHIKTKMLIDEGVEIKNGKILNLENTVYRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQM6</accession>
    <name>A0A2S2KQM6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Appr-1-p processing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_07540</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876614.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQM6"/>
    <dbReference type="GeneID" id="76208922"/>
    <dbReference type="OrthoDB" id="15450at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140291">
        <property type="term" value="P:peptidyl-glutamate ADP-deribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02901">
        <property type="entry name" value="Macro_Poa1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050892">
        <property type="entry name" value="ADP-ribose_metab_enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029494">
        <property type="entry name" value="DarT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12521:SF0">
        <property type="entry name" value="ADP-RIBOSE GLYCOHYDROLASE OARD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12521">
        <property type="entry name" value="PROTEIN C6ORF130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14487">
        <property type="entry name" value="DarT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52018">
        <property type="entry name" value="DART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-1277">Toxin-antitoxin system</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DarT" evidence="6">
        <location>
            <begin position="13"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS52018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="392" mass="45318" checksum="A9BFA71255FD5299" modified="2018-07-18" version="1">MDEFKIYTSDISRYLFYITHIDNIPSMLQNGILSHNLIEQENLDYTPIYDREIVSNRKEKMVNGKSLWHFANLYFQPRNPMLYRVTMEKSPDVIAVVAVDKKILDTSNAFITDGNAASEPTKFYPNTNFKIIEKQISRITDLQWWTESNATKRQIMAECLVPERIPPEFIRAIYVSNHELADKIRQSVSSSVSVIPEPSMFFQPVRKIPLTNNLSLVEGDLFFSKMQTLTVSVNCIGIMGKGLASRAKYQFPDVYVYYQDQCKRKTLRMGKPVLYQREGPYHQQIADDPSSLGNRTDTWFLLFATKQHWRDNSDINGIEKGLQWLLDNYERVGIKSLAIPALGCGLGRLRWEDVGPILCKYLSKMDIPVWVYLPAEKQLSNDLLTKEFLLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQM8</accession>
    <name>A0A2S2KQM8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ABM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_07810</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866840.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQM8"/>
    <dbReference type="GeneID" id="76208894"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007138">
        <property type="entry name" value="ABM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03992">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51725">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABM" evidence="1">
        <location>
            <begin position="18"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="110" mass="12788" checksum="959C56622EAF46EA" modified="2018-07-18" version="1">MKILEKKFNAYISSQNMFVMMADVNLKDGAEEDFKKWFSESNKVLANFPGFVSRRFLKANDGSYRIIVEHESKDTFIKMHNSPEHDKLHPEGHSYMSAPPERKTFSIIAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQP2</accession>
    <name>A0A2S2KQP2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator PadR N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_06680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866829.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQP2"/>
    <dbReference type="GeneID" id="76210027"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43252">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR YQJI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43252:SF5">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, PADR-LIKE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator PadR N-terminal" evidence="1">
        <location>
            <begin position="23"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="169" mass="19433" checksum="543EEE2E081D2091" modified="2018-07-18" version="1">MMISEWFQRVGSSVPRGFSRYFILELLKKKEITGKEIIDYAVEQSNGIWKPSPGLIYPLLGRLLDEELIDETKDGKYKLTKKGLDTAADVDKINDIVKKQLEVLFRLGNVGRFVALDLLEKISTMGSILSSNLTNMTDDETQKYKKFLQDELKKIEEKRSKKGKEIKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQQ3</accession>
    <name>A0A2S2KQQ3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07350</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQQ3"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="64" mass="7389" checksum="FB24A4A4F5E60229" modified="2018-07-18" version="1">MQKFFVSNNFDVNYSDLLVSYKIDVTRLSSSRVKNAIESQTGTIFQNNLTPFRIILSRTKKSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQQ6</accession>
    <name>A0A2S2KQQ6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">mRNA 3-end processing factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07840</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876639.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQQ6"/>
    <dbReference type="GeneID" id="76208891"/>
    <dbReference type="OrthoDB" id="40950at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050698">
        <property type="entry name" value="MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203:SF37">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="320" mass="35906" checksum="4128C92856F46782" modified="2018-07-18" version="1">MTKNGILCEVNEKRVYLDPKNSDVSGINFVSHAHSDHLPTKNGGMILSSIETNEIANLRGFKMKNHVESLENFSLINSGHILGAKGLLFDDIFYTGDICTRDRGFLKGAEIPKCKTLITECTFGLAEFVFPKMEQIQKHVNELISELYGKGIPVILMGYQLGKAQTITQLFGHWGPLYLHDSVKDMNLLHQKFGISLKDGMGHSEAEKNGLLEKKPWVMVAPMMSSKNKFVQEMKSKYGAVTIGFSGWAQSSRFSFGRRTDYSIPMSDHCDFNELVDMVIKSEAEQVYTIHGFVDEFAQHLKKIGISAQPLLENSLDNFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQS4</accession>
    <name>A0A2S2KQS4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ferredoxin--NADP(+) reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_06720</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876510.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQS4"/>
    <dbReference type="GeneID" id="76210032"/>
    <dbReference type="OrthoDB" id="35401at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051930">
        <property type="entry name" value="FNR_type-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47878">
        <property type="entry name" value="OXIDOREDUCTASE FAD/NAD(P)-BINDING DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47878:SF2">
        <property type="entry name" value="OXIDOREDUCTASE FAD_NAD(P)-BINDING DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="1">
        <location>
            <begin position="2"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="281" mass="31926" checksum="69C14474628B4546" modified="2018-07-18" version="1">MVVDNKATVTYVQLLKEDLVIIRLVPKEGPVPDYQAGQFITLGLPNPAEGGKIVRRAYSIASHPENRDYIELVIRWVRKPLPGRLTTQIFNTKEGDEILWLKPTGRALLINEELPNGEKDNRRIICIGGGTGLAPFVSYAQHLHDIGDKREIIVLHGASYVDELSYKDLLTGLENESIARGKDEWNFKYRAAISRPQEWFNRSWAGQVGRVETFLRPRDNGMSPLEELIGDKITKENTMFYVCGWQGTIDGVMDFLKPKGFVTEHDKREDGSFEVKYESYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQT2</accession>
    <name>A0A2S2KQT2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_07080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876575.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQT2"/>
    <dbReference type="GeneID" id="76208967"/>
    <dbReference type="OrthoDB" id="9682at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="170" mass="18309" checksum="417F73715E5BE905" modified="2018-07-18" version="1">MGLSNAISGGIIMFGITFVIFTFGGLTDKAASFSDASSETSDWESKLVKTSIDVSIPNDPGTDSTFSFDITNTNLEKLWEFEKFDIIITYNSAGIIQTETLTYDSTCAPNIGEWCISTWTNDIIDPKILNNGETITVDVEISNSIQSGSNLIVIVATQNGVVATKTRSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQX9</accession>
    <name>A0A2S2KQX9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PEFG-CTERM sorting domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08810</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQX9"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027560">
        <property type="entry name" value="PEFG-CTERM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04296">
        <property type="entry name" value="PEFG-CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="181" mass="20524" checksum="94C4BF7F955BF338" modified="2018-07-18" version="1">MIVPTNYAFAEHIFNPEAYAQYLDISQLESEKITFDFDGKSYDIYYGFHGSLDAMGSDDPLPTLHSMNINEERKSIEIIMEDVPEKTDFWIRTPQDVLYAEGEKFTVLVDGVDTGYDLMKFPNDYVVGFILSENTTHIEVIGTRVIPEFGGLAILILAISVTGIIYVIRKNPVLNGWTRIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQY2</accession>
    <name>A0A2S2KQY2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876701.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQY2"/>
    <dbReference type="GeneID" id="76208817"/>
    <dbReference type="OrthoDB" id="9797at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="128" mass="14963" checksum="EC272533A9CF7DA0" modified="2018-07-18" version="1">MIFPLLFKKGLSFVTFTLILIFFFGLVNIEHSALGISKPMFAITEQTIMIFDIIFWAIVCLLTVELVVAYLEIRNAKHFVKKYWLEIIMLVLMPIFVGFKILKITIKIIKQIKIMKTGFKLFKKIKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQY3</accession>
    <name>A0A2S2KQY3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD-binding FR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_06460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876490.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQY3"/>
    <dbReference type="GeneID" id="76210003"/>
    <dbReference type="OrthoDB" id="35401at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051930">
        <property type="entry name" value="FNR_type-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47878">
        <property type="entry name" value="OXIDOREDUCTASE FAD/NAD(P)-BINDING DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47878:SF2">
        <property type="entry name" value="OXIDOREDUCTASE FAD_NAD(P)-BINDING DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="1">
        <location>
            <begin position="2"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="280" mass="31896" checksum="993E14974E990493" modified="2018-07-18" version="1">MVTEMKAKVVYSELLKEDLVIIRLVPENGMPQYKTGQFLTIGLPIPSEKKIVRRAYSIASHAENRDYFEFVIRWVRKPLPGRVTTELFYLSVGDEVYLGEPTGAALQISDKLPNGQKDNRRVICVGGGTGLAPFIAFAKHFHDTNDKREVIVLHGASYVDELSYKRLLTDLELESEKRGRDKWNFRYRAAISRPKEYFNRSWNGHVGRVESFFQPNKKTGLSPVEEMVGEEITPENTIIYICGYQGTIDGVMEYLGPKGFVTEHEKKPDGSFAIKYESYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQY9</accession>
    <name>A0A2S2KQY9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_263970063.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQY9"/>
    <dbReference type="GeneID" id="76208807"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="1">
        <location>
            <begin position="3"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="63" mass="7056" checksum="4E601941DF705C6C" modified="2018-07-18" version="1">MIFTNILIAYDMSSFSNRAFKIALEIAKTNGSKITLLTIIPGEYSAIMGYSKINPQTIQKQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQZ9</accession>
    <name>A0A2S2KQZ9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Iron-sulfur cluster carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_08740</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds and transfers iron-sulfur (Fe-S) clusters to target apoproteins. Can hydrolyze ATP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876713.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQZ9"/>
    <dbReference type="GeneID" id="76208796"/>
    <dbReference type="OrthoDB" id="8297at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.130:FF:000020">
        <property type="entry name" value="Iron-sulfur cluster carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000304">
        <property type="entry name" value="Iron-sulfur cluster carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044304">
        <property type="entry name" value="NUBPL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961:SF2">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="6 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIP18 family-like" evidence="8">
        <location>
            <begin position="5"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="105"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="370" mass="39315" checksum="B4EDD8C986DE6859" modified="2018-07-18" version="1">MVGIDQVLEKLSTVIDPDLKKDIVSMGMIKDLELNDGDLKFTLELTTPACPFNVEIEDDVRKVIGEITDLKNFDLKVTAKVMEGRSLDADTGMATVKNIIGVASGKGGVGKSTVSLNLALALSESGAKVGLLDADIYGPSIPLMLGMKDGFMEVEDNKLQPADSNGLKVVSFGFFADQSNQAAIYRGPIISGILKQFLVDTNWSDLDYLIVDLPPGTGDIPLTLAQTIPITGILVVTTPQDVASNVAVKAVSMFEKLNVPIIGVVENMSYFVCPNCNDKHYIFGEGGAKKISEQFNMPFLGEIPLNSGIMAGSDLGKPIMITSPDSPSALAFRKSAKNIAAQCSILAAKLQDEMASESSSEESSPEASTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR06</accession>
    <name>A0A2S2KR06_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Trm112 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_06760</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876514.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR06"/>
    <dbReference type="GeneID" id="76210036"/>
    <dbReference type="OrthoDB" id="6467at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005651">
        <property type="entry name" value="Trm112-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03966">
        <property type="entry name" value="Trm112p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158997">
        <property type="entry name" value="Trm112p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="89" mass="10509" checksum="95D91FCECC310A81" modified="2018-07-18" version="1">MNKTMMDILACPIDKNHPLELFEIEEKDNVISEGALFCEKCSRFYPIIEGIPIMLPDELRDKKQEMDFLKNYKKELPEKIITQGNPWHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR13</accession>
    <name>A0A2S2KR13_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876731.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR13"/>
    <dbReference type="GeneID" id="76208776"/>
    <dbReference type="OrthoDB" id="375060at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="172" mass="19839" checksum="F28127C9F449428E" modified="2018-07-18" version="1">MNTRFQKQSREKKIIFFIVGLFVLNAFGMVPLGIIGAYTTPPMTITTTETLEDGAIQEKTSKKYYHEGLYETLQIHIYEFFRYSLPLTMTGYFLVDLPEFSYDYVGVIVWVLSLVSIPSVIKFTAQFPVWRRFIYVYLIAVQIAGMWALGRVNFECISFIDYPIRPGPPCGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR41</accession>
    <name>A0A2S2KR41_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_09100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR41"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050553">
        <property type="entry name" value="Thioredoxin_ResA/DsbE_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42852:SF13">
        <property type="entry name" value="PROTEIN DIPZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42852">
        <property type="entry name" value="THIOL:DISULFIDE INTERCHANGE PROTEIN DSBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="11"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="228" mass="25775" checksum="EFEDFF9069CA0894" modified="2018-07-18" version="1">MEKIQQSTMSAVIGEKVPNFGVSEWVQGAPTNFDQEKDHIVILEVFQVNCPGCFMHALPEAIEIYNKYKDEGVRVLGLATAFEDFDKNTLDNLKMLAETGEVIGETKNALSMYGQLKDGKLSYKIPFPLGMDNLTKTSGEISQEKIMQFIYPQIPDFDSQSEDYKKQIIQRVKDYMKSKEYSAETFEKFALQGTPSTIIVDRKGILRNVSFGQTGHIEPLIQKLLAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR42</accession>
    <name>A0A2S2KR42_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit Rpo7</fullName>
            <ecNumber evidence="4">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpo7</name>
        <name evidence="4" type="synonym">rpoE</name>
        <name evidence="6" type="ORF">NZNM25_08750</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the RNA polymerase complex. Forms a stalk with Rpo4 that extends from the main structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4">Forms 2 domains with an elongated structure; Rpo4 packs into the hinge region between the 2 domains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="4"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR42"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04331">
        <property type="entry name" value="RNAP_E_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04460">
        <property type="entry name" value="S1_RpoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00865">
        <property type="entry name" value="RNApol_arch_Rpo7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004519">
        <property type="entry name" value="RNAP_E/RPC8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046399">
        <property type="entry name" value="RNApol_Rpo7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005576">
        <property type="entry name" value="Rpb7-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006333">
        <property type="entry name" value="PRK08563.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00448">
        <property type="entry name" value="rpoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF4">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03876">
        <property type="entry name" value="SHS2_Rpb7-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="N-terminal, heterodimerisation domain of RBP7 (RpoE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 6" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S1 motif" evidence="5">
        <location>
            <begin position="66"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="182" mass="19589" checksum="33D04F8E63F4E635" modified="2018-07-18" version="1">MFGTTLKKAAVNILKEKYESMINADLGYIIMILDAKVDEMGKMIAGDGGTFHKVQFEALTFYPKLQEIVQGEIVDITDFGAFVRIGPTDALLHLSQVMDDYLKSDVKSGMILANQSGRTLKVGSTLRARITAVSLGKAAAMGKIGITCRQPFLGADDWIAEEIKKAGGGSDKPAKEAEVEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR52</accession>
    <name>A0A2S2KR52_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_09510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876785.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR52"/>
    <dbReference type="GeneID" id="76208719"/>
    <dbReference type="OrthoDB" id="52656at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.300.12:FF:000007">
        <property type="entry name" value="Iron-sulfur cluster assembly accessory protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016092">
        <property type="entry name" value="ATAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000361">
        <property type="entry name" value="ATAP_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017870">
        <property type="entry name" value="FeS_cluster_insertion_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00049">
        <property type="entry name" value="iron-sulfur cluster assembly accessory protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010147">
        <property type="entry name" value="PRK13623.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43011">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY 2 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43011:SF1">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY 2 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01521">
        <property type="entry name" value="Fe-S_biosyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01152">
        <property type="entry name" value="HESB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core" evidence="1">
        <location>
            <begin position="10"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="116" mass="12673" checksum="8EFDD89BE00EFDC4" modified="2018-07-18" version="1">MATEQTQKMITVTAKAAEKIKEFMKEEAESPEYLRVYVQGGGCSGLSYGMGFEKAPEEDDIVMEENGVKLVVDSYSVDHLQGANVDYIESLMGSGFKINNPNVTKSCSCGHSFSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR57</accession>
    <name>A0A2S2KR57_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Universal stress protein UspA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_07260</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876589.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR57"/>
    <dbReference type="GeneID" id="76208949"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF25">
        <property type="entry name" value="USPA DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="16"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="158" mass="17996" checksum="EBA73880E1509A2C" modified="2018-07-18" version="1">MQVNTPQQEKFSKAIFRKILVPFDNSKMSDRAFWHAINLNFKHKSEIIILSVFHSEQYSSSFLNYNTHQTIIEQKKLNEVKIKHKKLKEISEECGIPCRTFVATSSSITQTVMSYVYSTKADLVIMGTRGNGSDRKLMLGSVSLEISQNAPIPVLLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR83</accession>
    <name>A0A2S2KR83_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_09670</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866848.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR83"/>
    <dbReference type="GeneID" id="76208703"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="83" mass="9579" checksum="254EFA34699D7D86" modified="2018-07-18" version="1">MEARKIPDREEAILLDMTFCCKGICENLELTPVPTSLRYRLGHKRCTMCSIFVETEKLSCPCCGVRLRTKSRNKKHSEEELSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR93</accession>
    <name>A0A2S2KR93_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07660</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876626.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR93"/>
    <dbReference type="GeneID" id="76208910"/>
    <dbReference type="OrthoDB" id="3379at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="339" mass="40436" checksum="2C7658301F1DBD3C" modified="2018-07-18" version="1">MKDKTCDTKPTFQQNFPYHNDHAIEQSEPYANNFDNDDELYNDWELDDYHLLTNWETAEHNGWRLPATKEPHYWCAQWNHKGCIHSEDHQTHDGKIFIRRYQLSCFRPACKTCWEKWIGRQSNVATRKIERYSKKENISPIHIVLSVSNWDYELDYKQMKQKARKILNEIKIRGGSIIFHPFRFNKKIRCWYFSPHFHVVGFGFIPKGSLVDAYHQNGWFVKYLGVRKSVFATFYYLLSHCGIRHRTRATTWFGDLSYSKLEKEILPDFSKCPLCGRKLTEIYYKGWDPPIESNSFFEGFLDSEGWCVVITEKHIEPCNFEYAPTRELNNLLQSLEQAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRA2</accession>
    <name>A0A2S2KRA2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA protection during starvation protein</fullName>
            <ecNumber evidence="3">1.16.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_07760</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Protects DNA from oxidative damage by sequestering intracellular Fe2+ ion and storing it in the form of Fe3+ oxyhydroxide mineral. One hydrogen peroxide oxidizes two Fe2+ ions, which prevents hydroxyl radical production by the Fenton reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2 Fe(2+) + H2O2 + 2 H(+) = 2 Fe(3+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:48712"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homododecamer. The 12 identical subunits form a hollow sphere into which the mineral iron core of up to 300 Fe(3+) can be deposited.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Dps family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.16.-.-" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876633.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRA2"/>
    <dbReference type="GeneID" id="76208899"/>
    <dbReference type="OrthoDB" id="5651at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009295">
        <property type="term" value="C:nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01052">
        <property type="entry name" value="DPSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053475">
        <property type="entry name" value="DPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014490">
        <property type="entry name" value="Dps-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033921">
        <property type="entry name" value="DPSL_diiron-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040955">
        <property type="entry name" value="Arch_DPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009990">
        <property type="entry name" value="PRK13456.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30295">
        <property type="entry name" value="BACTERIOFERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30295:SF1">
        <property type="entry name" value="DNA PROTECTION DURING STARVATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018063">
        <property type="entry name" value="Ferrtn_UCP018063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="1 3" id="KW-0409">Iron storage</keyword>
    <keyword evidence="3 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin/DPS" evidence="6">
        <location>
            <begin position="28"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR018063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR018063-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="195" mass="21984" checksum="CA4A0BDDFB05CC64" modified="2018-07-18" version="1">MSESNTPNVVGINVLKQNGLDVDELLKELIKNAAVEFTAYYYFTNLRAHCTGLEGEGLKGIIEDARLEDLSHFESCLERIYQLGGALPNDATEFIKMSGCEFLQLPANPTDHKAILEKCLKAEQGAIVNWNKICLMTIGKDPATYDIAKDILAEEIEHESWFLELIYGRPSGHMRRKYSGERPHTRKHSRALDMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRB7</accession>
    <name>A0A2S2KRB7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histidine kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_07860</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866841.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRB7"/>
    <dbReference type="GeneID" id="76208890"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00075">
        <property type="entry name" value="HATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065:SF10">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065">
        <property type="entry name" value="SENSOR HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="144"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="348" mass="38949" checksum="09507012A34D80C6" modified="2018-07-18" version="1">MHREIKEDSELDPMNAPQRTVFFKKSKPAETIKIDFKSNEKSKKIDSLLEKAPEKNLELNHESLEENENLDESDKKLLTLKKEIETAQKFHQLTKQKLTAKSASLRKTKSTLTQTQKKLDEALSSKFTAVDNNKLEMIGKMSSKMAHDMRNPLTILQSQIELMKVKQKIHEDTVLSNSILSMENALSHITNQINDVLNFIKTPEIRLITCDLKEIVKSSIGEVRFPPDVELHSSLNSCVLQCDVVKIRGIVTNIIQNAVQAIGLKGKVSVSLEVDGEFATIKISDSGPGIPKENIEQIFEPMFTTKDDGTGLGLASCKQYLEMHKGTIDVSNNPTTFTITLPKTAESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRC0</accession>
    <name>A0A2S2KRC0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Agmatinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_10210</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="4">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the arginase family. Agmatinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876841.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRC0"/>
    <dbReference type="GeneID" id="76208646"/>
    <dbReference type="OrthoDB" id="7186at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033389">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via agmatine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005925">
        <property type="entry name" value="Agmatinase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01230">
        <property type="entry name" value="agmatinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="ARGINASE/AGMATINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358:SF26">
        <property type="entry name" value="GUANIDINO ACID HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036979">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR036979-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="282" mass="31058" checksum="5931A65623520495" modified="2018-07-18" version="1">MEKICWANTDNFDESEFVVLGIPDESQSHALRKGTEEAPSKIRQISNLRDSYVRNGTVSLGRPFQGNEKKVHDIGNINRTDIEKIYDKISTSSKIPISIGGDHSITSQIINSMANTYGKISLVYFDAHPDFVSSAANYYGSVITDVLSRIEIESSIQIGIRTPEQEELDNIKKYNLQVITPFDIAEQGIAKVANTILDKLGDKVYVSFDMDCIDPAHAPGVSVPVPVGLNSNDAIYLLQKIAKRGIIGMDIMEVCPSFDIKDRTSHLASRIISEVLYSSGGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRM7</accession>
    <name>A0A2S2KRM7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_10940</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876944.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRM7"/>
    <dbReference type="GeneID" id="76208564"/>
    <dbReference type="OrthoDB" id="25379at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004081">
        <property type="term" value="F:bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006167">
        <property type="term" value="P:AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006754">
        <property type="term" value="P:ATP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03428">
        <property type="entry name" value="NUDIX_Ap4A_Nudt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051325">
        <property type="entry name" value="Nudix_hydrolase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003565">
        <property type="entry name" value="Tetra_PHTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21340:SF0">
        <property type="entry name" value="BIS(5'-NUCLEOSYL)-TETRAPHOSPHATASE [ASYMMETRICAL]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21340">
        <property type="entry name" value="DIADENOSINE 5,5-P1,P4-TETRAPHOSPHATE PYROPHOSPHOHYDROLASE MUTT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="6">
        <location>
            <begin position="2"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="139" mass="16545" checksum="89FF87B0F005D0CE" modified="2018-07-18" version="1">MIEETSAGIVIFRKEDSKNLFLLLHYPSGHWDFVKGKMEVNETTHQTAIRETREETGITDIRFIENFEEWIEYNFQYQGELIHKKVVFFLAETKTEKVEISHEHQNYTWMDYNTAMEKTTFDNAKTVLTRAQMLLSKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRN7</accession>
    <name>A0A2S2KRN7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">6-pyruvoyl tetrahydropterin synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_11310</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876974.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRN7"/>
    <dbReference type="GeneID" id="76208526"/>
    <dbReference type="OrthoDB" id="6529at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.10">
        <property type="entry name" value="6-pyruvoyl tetrahydropterin synthase/QueD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007115">
        <property type="entry name" value="6-PTP_synth/QueD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038418">
        <property type="entry name" value="6-PTP_synth/QueD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589:SF7">
        <property type="entry name" value="6-PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589">
        <property type="entry name" value="PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01242">
        <property type="entry name" value="PTPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="271" mass="31100" checksum="F0F799AE5A207EC8" modified="2018-07-18" version="1">MTASPTILDSDFRYIDKKGNLLKTRTELTVAQMLTFLEEEYQYNHKIILKNGIEVSVDFKTEKGLIEVIDTDEDIEKYKQIKEDFPQEKVMAIGHAKYVAQIKELQDVVFYDKTPQTGSIFLEDASFSFDYAHILPLVEKCSILHGHTSSVMVELVGQMKDNLLLDFGEAKKIIKEVVNVFDHKFFINRKYLKKEDDSHYHIQFEGPKGMFELQVPKNTTYLLEGEATVENLSSEIIKLLAPKMPSNVEAVGVYIYEGYNKGSHIISNISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRQ6</accession>
    <name>A0A2S2KRQ6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Type 2 DNA topoisomerase 6 subunit A</fullName>
            <ecNumber evidence="9">5.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Type II DNA topoisomerase VI subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">top6A</name>
        <name evidence="14" type="ORF">NZNM25_10850</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Relaxes both positive and negative superturns and exhibits a strong decatenase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9 10">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer. Heterotetramer of two Top6A and two Top6B chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9 10">Belongs to the TOP6A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="9"/>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_370684784.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRQ6"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00223">
        <property type="entry name" value="TOPRIM_TopoIIB_SPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00132">
        <property type="entry name" value="Top6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002815">
        <property type="entry name" value="Spo11/TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013049">
        <property type="entry name" value="Spo11/TopoVI_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036078">
        <property type="entry name" value="Spo11/TopoVI_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004085">
        <property type="entry name" value="TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034136">
        <property type="entry name" value="TOPRIM_Topo6A/Spo11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003335">
        <property type="entry name" value="PRK04342.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848:SF0">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21180">
        <property type="entry name" value="TOP6A-Spo11_Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04406">
        <property type="entry name" value="TP6A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01550">
        <property type="entry name" value="TOP6AFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01552">
        <property type="entry name" value="TPISMRASE6A"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56726">
        <property type="entry name" value="DNA topoisomerase IV, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52041">
        <property type="entry name" value="TOPO_IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 9 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 10" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Spo11/DNA topoisomerase VI subunit A N-terminal" evidence="12">
        <location>
            <begin position="84"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Topoisomerase 6 subunit A/Spo11 TOPRIM" evidence="13">
        <location>
            <begin position="202"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="9 10">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF21180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GBH34294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="372" mass="42496" checksum="839CC1CB630B59A6" modified="2018-07-18" version="1">MKTNKEKNKIKDRSKKADEKQKNILEMLKSHGAKIYEDLENGQFPKFSIPSRSVSNIVYDKNLRQYILGNNSAIRSSRNSSQLRSFTQLMWLAFFANRLTQEKKSSTLRDVYYSSQAFAIEFEDQSESDNIIVDLEAVTSKPREDFHIFPEERSSIFGDLNIEYTIPGYEGKTMNLSNHPDGYSIGPSLTTAELVDTSAEIVIAIEKGGLFTRFVEEQIDKKFKSIIINTGGQAPRSTRTLLKRLHEEMGLPVIVLTDGDVYGEHIAMVIKSGSANAAHLRELTVPDAKWVGVWATDIEKYKLPTIPMTESDIKRCYDLQKDPRYEDGIWKKELDVFLRLKRKAELEAFSKYGLTNITEKYLPQKLELAKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRR2</accession>
    <name>A0A2S2KRR2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_09290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876763.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRR2"/>
    <dbReference type="GeneID" id="76208741"/>
    <dbReference type="OrthoDB" id="2005at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="110" mass="12515" checksum="EAB6F235CE6E8815" modified="2018-07-18" version="1">MSEVEIHYNDFLSSLGQLITNVDVNLMMKVMYLERKLPDVDPKVELHIDYKSGVDLMKKQEQMRGKYGFPTQTGDHALTVVGRMNIDLIEEISKDQDIEHVSGNATPASY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRT9</accession>
    <name>A0A2S2KRT9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_10780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRT9"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="9"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="218" mass="25548" checksum="F4D88C555A49971E" modified="2018-07-18" version="1">MNSGKKHTVIVRQMEEKDIPQIVELQKIAFPSIASKGIYWKPKHLKMHLQVFPEGQFVAEYKEKIVGSCSSLIITLHPEYKEHTWMSVCGNSFFENHDPRGDTLYGADISSHPDYRRLGIATKLYNARKNLATKLNLRRIIAGGRILNYCEHAKNMSPLEYVQKVKLHEIKEPVLLFQFRNKFKFIKILPNYMKDPRSLNYATFIEWKNPNFIEKNDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRU8</accession>
    <name>A0A2S2KRU8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11910</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRU8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="67" mass="8134" checksum="E79C6FE212C9ACC2" modified="2018-07-18" version="1">MLTEIIIEKLFEQRDFYLNTIKHLEFQLIMEPTPDEILQNEKLKIITIKELKKIESEIRLILSEKTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRX9</accession>
    <name>A0A2S2KRX9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_10920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876943.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRX9"/>
    <dbReference type="GeneID" id="76208566"/>
    <dbReference type="OrthoDB" id="31282at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007254">
        <property type="entry name" value="DUF373"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38815:SF1">
        <property type="entry name" value="DUF373 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38815">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED, DUF373 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04123">
        <property type="entry name" value="DUF373"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="280"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="347"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="381" mass="41397" checksum="BD86AEA0F84EDA8E" modified="2018-07-18" version="1">MSQQSDKVEKDVNASAANRLLVICIDRDNDVGEKARIATPVIGRDACIEAAQRLALEDPEDADSNSIFAAIKTYEDLISKGYQVEVITVAGVKDRGVQADEKILAETRKVLENFSANGAVIVSDGEDDESVIPVIQNVLPVVSVQRVVMKVSRSVEYSYAVFGKYLKMLAYDSKYSKFFLGVPGILLLIGGIATAFGYTAEIFAVLVSILGGAFLIRAFDIDRALSNWSKPTPMGFIRMFTMVAGILLILSSVPAGINSVDSELIEADTQLISKITDKMIIGQFVVGALPILWIGMGAIFAGTLLSNWIGGIPRQISDSLRIIVLISLYPTVAQFTNIMIYEESSFTLIPPLLGGLAATLISATILFKKYRKHKNQEMISD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS28</accession>
    <name>A0A2S2KS28_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ammonia monooxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048097271.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS28"/>
    <dbReference type="GeneID" id="24819638"/>
    <dbReference type="OrthoDB" id="5728at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22188">
        <property type="entry name" value="arch-AMO_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.50">
        <property type="entry name" value="Particulate methane monooxygenase subunit c2. Chain: C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006980">
        <property type="entry name" value="NH3_CH4_mOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023349">
        <property type="entry name" value="NH3_CH4_mOase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04896">
        <property type="entry name" value="AmoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="21090" checksum="11E72DB5014DBF54" modified="2018-07-18" version="1">MAQMPALIPKEVEIQRLKKIWLIVIAMGSTAASVEVDNFVDGSLHQTSIRDSAFTPAHWWLYSHFITLPLGWGAAAIYDRKVPVLRGPNNSMNTGLKMTILGYLATMFTIGVNEMWHFWFVEEIFAVPNHWMFNMGVVVAFMGALAYVVRVYARLVELGAETPGENPYVAEMYKMALEGKLYSRSIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS73</accession>
    <name>A0A2S2KS73_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DOT1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11920</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877044.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS73"/>
    <dbReference type="GeneID" id="76209314"/>
    <dbReference type="OrthoDB" id="7386at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="275" mass="31241" checksum="A178B7F4A387E304" modified="2018-07-18" version="1">MLNQIVKNLILIKQDFVKNYSGSAHIQEVIPFQPSKEFPIAEDHLNLLHYFAKQNPIYFNSFEQQIAGISCTVYEGDINEHWLNSIKHGTSCQPFYPTWIMSAYIMAFFAKQLGYSELVDIGSGDGRIAFCGTFLKLISHSIEIDDVLVKLQDTLCQSTHQNFHPKCEDALEFDYSKIKLIHPVFFIGGLPQMGGDVLATNIIDKINSMPDLKNNTCIVFAGTHSKRHLSGNLENGGWSTLIEKHNLDVIDTISLPTVWTFDQEIETPYIYTKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS78</accession>
    <name>A0A2S2KS78_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877137.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS78"/>
    <dbReference type="GeneID" id="76209186"/>
    <dbReference type="OrthoDB" id="17596at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="70" mass="7921" checksum="DF978D29011A1D8B" modified="2018-07-18" version="1">MGIVSKGAKCNVEGCDKDGARSLNTTKVENAGLRVNSTGKKTVLCKEHYKEWKKESKDDRDLERARFDKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS93</accession>
    <name>A0A2S2KS93_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">NZNM25_12750</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877109.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS93"/>
    <dbReference type="GeneID" id="76209226"/>
    <dbReference type="OrthoDB" id="12029at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562">
        <property type="entry name" value="NAPA-TYPE SODIUM/HYDROGEN ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562:SF3">
        <property type="entry name" value="SODIUM ION_PROTON EXCHANGER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="10" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="298"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="358"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="12">
        <location>
            <begin position="477"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH34484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="535" mass="59201" checksum="3C1B43848CB40560" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSE6</accession>
    <name>A0A2S2KSE6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Exosome protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877005.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSE6"/>
    <dbReference type="GeneID" id="76208483"/>
    <dbReference type="OrthoDB" id="10874at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002739">
        <property type="entry name" value="PAB1135-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38816:SF1">
        <property type="entry name" value="EXOSOME SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38816">
        <property type="entry name" value="EXOSOME SUBUNIT, DUF54 FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01877">
        <property type="entry name" value="RNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="16573" checksum="5E6C189DB8039F94" modified="2018-07-18" version="1">MIDKIEVTIDVIVHATEDISKIFQAFELLNLKKEDFTIHETTGYFENPITMLNAKIVKKEAENFMKKLLGLLSHQQINELIEEIEERTVDSRFHMRLDKQELIKGNVVIREKDTIKIKIHTPIYNKKETVKTFTEIFQIAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSF0</accession>
    <name>A0A2S2KSF0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">anthranilate synthase</fullName>
            <ecNumber evidence="2">4.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pabA</name>
        <name evidence="8" type="ORF">NZNM25_12880</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877116.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSF0"/>
    <dbReference type="GeneID" id="76209212"/>
    <dbReference type="OrthoDB" id="3321at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000003">
        <property type="entry name" value="Anthranilate synthase component II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050472">
        <property type="entry name" value="Anth_synth/Amidotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418:SF4">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="7">
        <location>
            <begin position="4"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="201" mass="22236" checksum="D5E45433923082D6" modified="2018-07-18" version="1">MKFLIIDNYDSFVYNIAQYLGELGVDCEVIRNDKITLDQIKQNNYDGIIISPGPGTPEDKKYFGVCSDVIKNIGPTTPILGVCLGHQGIIHAFGGKVTNAGCVRHGKTSPVKHTNSGLFLNVKNPFRATRYHSLVGDKTKIPDDLEVTATADDDGEVMAITHKKYLIQGVQFHPESIMTENGKKILENFISQVKEKKSRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSG1</accession>
    <name>A0A2S2KSG1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RDD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_14110</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSG1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051791">
        <property type="entry name" value="Pra-immunoreactive"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010432">
        <property type="entry name" value="RDD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36115:SF4">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36115">
        <property type="entry name" value="PROLINE-RICH ANTIGEN HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06271">
        <property type="entry name" value="RDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="RDD" evidence="7">
        <location>
            <begin position="19"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="165" mass="19085" checksum="110D879928D37BB8" modified="2018-07-18" version="1">MNIESMDESNAEGSKIIIAKWTDRFFAWLIDFIIVSSISTVLIFVSFGSLDYEFEENSFWMENIQYIPTSILFFVYWTVLEYKIGQTVGKKIMNLKTVNIIGERPSLKGILISSFGKSFLLPIDVVLGWFLTNEKRQRIFNKLGDTLVVKIKDVDDVSDIKYLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSI5</accession>
    <name>A0A2S2KSI5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">30S ribosomal protein S26e</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_13020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSI5"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1740.20:FF:000004">
        <property type="entry name" value="30S ribosomal protein S26e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1740.20">
        <property type="entry name" value="Ribosomal protein S26e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000892">
        <property type="entry name" value="Ribosomal_eS26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047864">
        <property type="entry name" value="Ribosomal_eS26_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038551">
        <property type="entry name" value="Ribosomal_eS26_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006816">
        <property type="entry name" value="PRK09335.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12538">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12538:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01283">
        <property type="entry name" value="Ribosomal_S26e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00733">
        <property type="entry name" value="RIBOSOMAL_S26E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="99" mass="11075" checksum="0FC053FB3A1C7F40" modified="2018-07-18" version="1">MFILMPLKRASRGRTKGGKGSSGTVQCTNCGQTVPKDKAKKVTSRLNLVEHTLAKELRAQGAYIASPTVLKWYCISCAIHFKILKIRSEDSRRKRGKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSI8</accession>
    <name>A0A2S2KSI8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit Rpo12</fullName>
            <ecNumber evidence="8">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit P</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rpo12</name>
        <name evidence="8" type="synonym">rpoP</name>
        <name evidence="9" type="ORF">NZNM25_14410</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the archaeal Rpo12/eukaryotic RPC10 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="8"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSI8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.30">
        <property type="entry name" value="RNA polymerase ii, chain L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00615">
        <property type="entry name" value="RNApol_arch_Rpo12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006591">
        <property type="entry name" value="RNAP_P/RPABC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023464">
        <property type="entry name" value="Rpo12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00659">
        <property type="entry name" value="RPOLCX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="RNA polymerase subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 8" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 8" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="65" mass="7397" checksum="EFFEF057FB094E7D" modified="2018-07-18" version="1">MVENFDDDEIEETPVETFDVNYSCLRCGTIVSNTELSRLPEIKCICGFRVFTKVRPPVVKTVKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSN4</accession>
    <name>A0A2S2KSN4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DEAD/DEAH box helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_13480</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_370684789.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSN4"/>
    <dbReference type="GeneID" id="76209151"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12089">
        <property type="entry name" value="Hef_ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1320.20">
        <property type="entry name" value="hef helicase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041755">
        <property type="entry name" value="Hef_ID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14025">
        <property type="entry name" value="FANCONI ANEMIA GROUP M FANCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14025:SF20">
        <property type="entry name" value="FANCONI ANEMIA GROUP M PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="15"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="328"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="493" mass="55507" checksum="2FE8854732A7705D" modified="2018-07-18" version="1">MQKNSIEKRDYQVNLANQAIEENCIVVLPTGLGKTAIALQVIAEYLSRGSGGILFLAPTRVLVNQHFEFLKDNLTLDDISLITGEDPISKRTKLWNNSVICATPEIARNDLVRGIITPDQFSLVIFDEVHRTAGDYAYSGIAESFENSTARILGMTATLPSEKEKATEILTKLRISSVAERREDSPDVKPYTQETHTEWINVELPPELKSIQKLLKLALDERYETLRKNGIRMAEQQSLSVLLRIRQFVLNQNRRSAKPLFTGIRIHYALNILEAHGITPFLKFCERVQAKKGVGIKELFEVDPNFTRAIHLAKEAQSRGIEHSKIPKLKEILESVPGKALIFTSYRDSVDLIFNKLTEMGISAGILIGKAGDAGLKQKKQIETVQKFRDGIFQVLVATRVGEEGLDIAEVNQVIFYDNVPSSIRFIQRRGRTGRKDIGKLVVLIAKNTIDETYYWIGKRKMTAAKSMGDKMTKLLEKNHEIESQKTGLDAFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSN5</accession>
    <name>A0A2S2KSN5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 15">Fructose-1,6-bisphosphate aldolase/phosphatase</fullName>
            <shortName evidence="15">FBP A/P</shortName>
            <shortName evidence="15">FBP aldolase/phosphatase</shortName>
            <ecNumber evidence="6 15">3.1.3.11</ecNumber>
            <ecNumber evidence="15">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">fbp</name>
        <name evidence="17" type="ORF">NZNM25_14440</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes two subsequent steps in gluconeogenesis: the aldol condensation of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GA3P) to fructose-1,6-bisphosphate (FBP), and the dephosphorylation of FBP to fructose-6-phosphate (F6P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 15">Homooctamer; dimer of tetramers.</text>
    </comment>
    <comment type="domain">
        <text evidence="15">Consists of a single catalytic domain, but remodels its active-site architecture via a large structural change to exhibit dual activities.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the FBP aldolase/phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="6 15"/>
    <dbReference type="EC" id="4.1.2.13" evidence="15"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877247.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSN5"/>
    <dbReference type="GeneID" id="76209051"/>
    <dbReference type="OrthoDB" id="5829at2157"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02067">
        <property type="entry name" value="FBP_aldolase_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002803">
        <property type="entry name" value="FBPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036076">
        <property type="entry name" value="FBPase_V_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041126">
        <property type="entry name" value="FBP_aldo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38341">
        <property type="entry name" value="FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE/PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38341:SF1">
        <property type="entry name" value="FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01950">
        <property type="entry name" value="FBPase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015647">
        <property type="entry name" value="FBPtase_archl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111249">
        <property type="entry name" value="Sulfolobus fructose-1,6-bisphosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 15" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 15" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="10 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="11 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 15" id="KW-0704">Schiff base</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="358"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for FBP phosphatase activity" evidence="15">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor; for FBP aldolase activity" evidence="15">
        <location>
            <position position="225"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with DHAP; for FBP aldolase activity" evidence="15">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="101"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="238"/>
            <end position="239"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GBH34653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="378" mass="41411" checksum="199D1C39BBEE54FC" modified="2018-07-18" version="1">MKITVSVIKADVGGVGGHTKPSDGLIEAIRNTVKNSGDLLIDHYIGYCGDDTHIVMTHTHGVDNEKIHKLAWDAFMAGTQVAKEEGLYGAGQDLLKDSFSGNVKGMGPGVAEMEFEERPNEAFTVFAADKTEPGAFNYPIYRMFVDALSNTGLIVNKSLAAGVKMHIMDVEKAQIAELELWQDKPTIEAALMYPGRYVVDSVYTKDGEPILDASTDRLHNIAGTYVGKDDPICLVRTQKNFPATEEVGSVFNNPHYVAGNTRGSHNMPLMPVKLNSAATINFCIPIVQALVFSMHNGKLTGPFDGFSTPDWDYIREIATKKAIAIRSQGFIHPATLVPSELEYAEGYRARMDVLEAKMKPMEEASSSGEKKENYEDPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSR0</accession>
    <name>A0A2S2KSR0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_14740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877273.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSR0"/>
    <dbReference type="GeneID" id="76209021"/>
    <dbReference type="OrthoDB" id="1345at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="91" mass="10608" checksum="D665F1DF4B5ABF27" modified="2018-07-18" version="1">MVDDRTRCKECYIKKRHLGWKKLIRKNKVVLIFVAILWLYAVFPGPFIPGLDQTFYTISLVAALLIMIPIGLALFFWSLNPPKSDVKKRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSR3</accession>
    <name>A0A2S2KSR3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S24/S26A/S26B/S26C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_15030</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866906.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSR3"/>
    <dbReference type="GeneID" id="76208993"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015927">
        <property type="entry name" value="Peptidase_S24_S26A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001733">
        <property type="entry name" value="Peptidase_S26B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02228">
        <property type="entry name" value="sigpep_I_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806:SF6">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00717">
        <property type="entry name" value="Peptidase_S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S24/S26A/S26B/S26C" evidence="7">
        <location>
            <begin position="29"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="274" mass="30963" checksum="BB1F197960F33701" modified="2018-07-18" version="1">MIQKKIKILVTLLFIPILIYFWPVQLYGDTTYIMLVGNSMYPVIESGTFVVLKQEQQYFLGDIIGFVNEDNKNVVHRIVEQTDEGFITKGDNNKKNDPGVIPVDKVVGRSIFVIPYVGYTSLFLQTPIGMSIFGIWALIMFNKNKSKTNNKKSSESFIIFKIGMISVVINYVLTQVTLGFDIRLSKTMNIPFSNIFEPTIANSVSFGMLTMAIIMLYLFSRKIQDNKSDEIKPLKLIFALGGIMILVLQIISAINTIPILITNISENQLIPPIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSY2</accession>
    <name>A0A2S2KSY2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcriptional regulator MntR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mntR_2</name>
        <name evidence="7" type="ORF">NZNM25_14580</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DtxR/MntR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877260.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSY2"/>
    <dbReference type="GeneID" id="76209037"/>
    <dbReference type="OrthoDB" id="24735at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000189">
        <property type="entry name" value="HTH-type transcriptional regulator MntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.60.10:FF:000005">
        <property type="entry name" value="Transcriptional regulator MntR protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.10">
        <property type="entry name" value="Iron dependent repressor, metal binding and dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050536">
        <property type="entry name" value="DtxR_MntR_Metal-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001367">
        <property type="entry name" value="Fe_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036421">
        <property type="entry name" value="Fe_dep_repressor_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022687">
        <property type="entry name" value="HTH_DTXR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022689">
        <property type="entry name" value="Iron_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238">
        <property type="entry name" value="IRON (METAL) DEPENDENT REPRESSOR, DTXR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238:SF7">
        <property type="entry name" value="IRON-DEPENDENT TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02742">
        <property type="entry name" value="Fe_dep_repr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01325">
        <property type="entry name" value="Fe_dep_repress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00529">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47979">
        <property type="entry name" value="Iron-dependent repressor protein, dimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50944">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH dtxR-type" evidence="6">
        <location>
            <begin position="24"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="154" mass="17631" checksum="9B9DCF86E5EF528E" modified="2018-07-18" version="1">MKSKNSKRLDSIKAAHQTERTRSSARMEDYLEIISELVELKGYATTLDISRYMNVSAPSVTKMLQRLDEGGFLEYEKYHGINLTAKGSQIAEGIRQNHGILLEFFEILGVGYDTANQDTEGIEHHLNPKTIRQLRKFITFLKANPKIIENFKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSZ6</accession>
    <name>A0A2S2KSZ6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">2-(3-amino-3-carboxypropyl)histidine synthase</fullName>
            <ecNumber evidence="3 10">2.5.1.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">NZNM25_15570</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the first step of diphthamide biosynthesis, i.e. the transfer of the 3-amino-3-carboxypropyl group from S-adenosyl-L-methionine (SAM) to the C2 position of the imidazole ring of the target histidine residue in translation elongation factor 2 (EF-2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-histidyl-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36783"/>
            <dbReference type="Rhea" id="RHEA-COMP:9748"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="EC" id="2.5.1.108"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DPH1/DPH2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.108" evidence="3 10"/>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877388.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSZ6"/>
    <dbReference type="GeneID" id="76208430"/>
    <dbReference type="OrthoDB" id="314at2157"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11850">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042264">
        <property type="entry name" value="DPH1/DPH2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035435">
        <property type="entry name" value="DPH1/DPH2_euk_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022428">
        <property type="entry name" value="Dph2_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03682">
        <property type="entry name" value="arCOG04112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762:SF1">
        <property type="entry name" value="2-(3-AMINO-3-CARBOXYPROPYL)HISTIDINE SYNTHASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004967">
        <property type="entry name" value="DPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR004967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="331" mass="36830" checksum="104678613EB06F83" modified="2018-07-18" version="1">MIIIDEERIFKEIEKKNPSSVSLNGPDGILPQVQETAMRISEKFGIPAYVLADTTWGTCDLNTNGSKVLGAEIQFNIGHTINTESLEKNLVLIDAYDDVEFDSVAKKCPEILKGKTISLVTDSQHLHQVDKVEKILTENGIKVKIGKGKGQLNDGQVFGCEFYPASELKKEVDAYVFLGQSNFHAAGIALSTNLPTYILDPYFNEVREITEFAQKLKKKATLAIYKAAEAKTFGVIVGLKEGQLSKVFALKIKKELEKEGKKVQLFGLTDITNDRLQNLKGIDAFVQVACPRISTDNQFDKPVLSSPQANALLKILRNESIEEYLEIPHWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT11</accession>
    <name>A0A2S2KT11_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_15670</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877396.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT11"/>
    <dbReference type="GeneID" id="76208420"/>
    <dbReference type="OrthoDB" id="31397at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="191" mass="22096" checksum="FD9E56201E1FF69B" modified="2018-07-18" version="1">MKFSEEQVKEIVAMKESLVEQIDKHQESIEMLEKNITVLDLFLKDSSFTKASQLEMKKGPETHSKPEIIENPVENSIPIKRVNDGKIIANAYVTPEQLSIVLDNEIMINADTPPFKSFFLDRIIGEMKKKDFQEAENGKIQKESIIDYIINKNGSDIREIIIKNYRQKERVNELINTAGWSLTRMLENIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT80</accession>
    <name>A0A2S2KT80_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_16430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877470.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT80"/>
    <dbReference type="GeneID" id="76208342"/>
    <dbReference type="OrthoDB" id="12184at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049886">
        <property type="entry name" value="CFI_box_CTERM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041770">
        <property type="entry name" value="CFI_box_CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="272" mass="30088" checksum="FFF3A20C7A4964FB" modified="2018-07-18" version="1">MKFLFVLLLIPLLIIPAFAESQILPTEKGTLDVKLTYDEIDPNIQTKINIDFINPQTQKIQEHIDYTVSVSKDGETVFGPIPLTHTSIGSVKIPIEFNLGEGVYTMSFNIEGILFQPIPAEAVSFDIVVGEAQAQPMIPPENGVNGENGGCLIATATYGSEMAPQVQQLRELRDNTILSTESGIVFMTGFNQFYYSFSPAIADFERENPIFKEAVKLALTPMLTSLSLLNHVSIDSEEEMLGYGISIVLLNIVMYAGIPVFGILKLYQFRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT95</accession>
    <name>A0A2S2KT95_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_16470</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC-3 integral membrane protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT95"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="entry name" value="ABC transporter involved in vitamin B12 uptake, BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001626">
        <property type="entry name" value="ABC_TroCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30477">
        <property type="entry name" value="ABC-TRANSPORTER METAL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30477:SF0">
        <property type="entry name" value="METAL TRANSPORT SYSTEM MEMBRANE PROTEIN TM_0125-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00950">
        <property type="entry name" value="ABC-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="ABC transporter involved in vitamin B12 uptake, BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="272" mass="28680" checksum="A978EFE17AA1BD78" modified="2018-07-18" version="1">MSLEILTFSFMHRALISGIAIAILCSVVGLFLVLRRYSLFGDAIAHSSFGGIALGLLAGIYPLWTAYGVSIASALVITKIKNRYNISGDASIAVLLSSGIAVGLVIIGLSGGFTIDIFSFLFGSILLVSVNDTVLILALTGGILIVILLLYRQILYSTFNEEQAKVSGIPVEKINYLIVFMAGITVVTSIQLVGVLLISALFVIPNVTAIMYGKGFKQTAIISMSFSIFSVVVGILLSYIFDITPAGTIVLLAISLLAGTMGIKSSGLLSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTA4</accession>
    <name>A0A2S2KTA4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ammonium transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">amt-1</name>
        <name evidence="10" type="ORF">NZNM25_16640</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877489.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTA4"/>
    <dbReference type="GeneID" id="76208321"/>
    <dbReference type="OrthoDB" id="10960at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029:SF10">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="263"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="301"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="364"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="419"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="463"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="9">
        <location>
            <begin position="73"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="519" mass="54300" checksum="3C7E272A50719492" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTB0</accession>
    <name>A0A2S2KTB0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF922 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_16750</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146196007.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTB0"/>
    <dbReference type="GeneID" id="76208310"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="212" mass="24274" checksum="63A65CB50F51FCBD" modified="2018-07-18" version="1">MTTVKHWYVYMAAAVTVAFLVGFFWDQTPIPPNQIPWNSNTSLEWNDFQGTPPQTTPYSAATGYYLGYKDLVWRQYFVDNVCTFVISSVDVDAYVDRTLSWVKDGSQSSELLSHEQGHADILHVKALVLGKALWALVGMPHQCPDNDPAKVQQEVEDMVNSIYNPIFDSHDRLQDEYDSDTDHGRNPDEQRRWNQRLSDIFMPPPPNPIIPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTC2</accession>
    <name>A0A2S2KTC2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_15980</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTC2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21149">
        <property type="entry name" value="PUA_archaeosine_TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.90">
        <property type="entry name" value="ArcTGT, C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029402">
        <property type="entry name" value="TGT_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038250">
        <property type="entry name" value="TGT_C2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14810">
        <property type="entry name" value="TGT_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88802">
        <property type="entry name" value="Pre-PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA-guanine transglycosylase patch-forming" evidence="2">
        <location>
            <begin position="16"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="80"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="159" mass="17727" checksum="5ED117BA6B9FD3EB" modified="2018-07-18" version="1">MHMDQVLKLQYSLDALFGNGVSKYLPKNIEMIFSRKTGRIRTVLHEGKLLCTLRIDGGLAISPYFAQILLKSKTFKENCVEINKDAAPFVMDGKSVFCKHVVWCGNKVRISADTPVLFKGRVIAVGKAVLSHEMISDFNRGVAIKVRDSLKSPKEAIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTI4</accession>
    <name>A0A2S2KTI4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_15590</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877390.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTI4"/>
    <dbReference type="GeneID" id="76208428"/>
    <dbReference type="OrthoDB" id="8709at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08266">
        <property type="entry name" value="Zn_ADH_like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051603">
        <property type="entry name" value="Zinc-ADH_QOR/CCCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44154">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44154:SF1">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="2">
        <location>
            <begin position="17"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="359" mass="39341" checksum="F886CE152FCA5C9A" modified="2018-07-18" version="1">MKALVYDEYTTDDDFSKILKIKDLPLPKPKSNEVVFKVNAAALNYDDIWGMRGKPLAIPLPHISGTDAAGEVIAVGKDVKNVKVGDRIVSHGNMSCRVCKACTDGREYDCRKRAIWGFETGPLWGGYCEYTHLPEVNVVKIPDGVSYDEAAAASMTLLTSWHMLVGRAKIQPGQIVLVMGGSSGVGNYGIQIAKLFGCIVIATASPDKLDKLLELGADFAVDHRKEDWHKEVRSIAKKIPKQYGDVPGVDVIFEHIGGTHWNKELTLLNYGGTIVTTGATTGYDAKTDLRHIFFKGINILGSTQGTRSELEQGLYWMSQGKIKSVIDSVFPLEEAAEAHRKMLTGKGLFGKIIMRPNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTK8</accession>
    <name>A0A2S2KTK8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SpoVT-AbrB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_17750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877580.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTK8"/>
    <dbReference type="GeneID" id="76208210"/>
    <dbReference type="OrthoDB" id="30861at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01439">
        <property type="entry name" value="lp_hng_hel_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="10"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="53" mass="6052" checksum="EDA74BD6257789B3" modified="2018-07-18" version="1">MSVQDNEVLVKITSAGTISIPKQFRKYMDIQKGEYVKVILGKDRLLIRKVTIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTK9</accession>
    <name>A0A2S2KTK9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_17730</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877665.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTK9"/>
    <dbReference type="GeneID" id="76208212"/>
    <dbReference type="OrthoDB" id="9328at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="120" mass="13903" checksum="2EEF6B4CF568381D" modified="2018-07-18" version="1">MTELERSNFNNIIRKIIKKSLFTERQIEIILNQKNLLESDFSITKGAYYRQVGQSREKLIGLFYSIILLRGLGILLPDDIDVISKLSEQISVINDSDIFPEREDEVISVIDRLVRQACNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTV0</accession>
    <name>A0A2S2KTV0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_18630</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146196010.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTV0"/>
    <dbReference type="GeneID" id="76210091"/>
    <dbReference type="OrthoDB" id="387555at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="236" mass="26491" checksum="E84B2278CF4A4565" modified="2018-07-18" version="1">MTTKTIKTILFASLIAAMILPFSGMNYAVAEEVDKEYVEKIANKATALMEQLETEQNPNKIDRMQKQLERLLVILNSVGLYTEEQFEVIKEERQYDTPEETSESIQSFCPSCDDAVKVKVGFDYKLWGFYGTANGNWDTITSADPNGFSQALVGSWGADYAHTWYQYYLANGANGANVNFTPYITNSNDGLVHNYGTFQQTVSSTTIQQDYPSPVAYTPIYGNDKVKLVAVLQSIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU18</accession>
    <name>A0A2S2KU18_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA 2',3'-cyclic phosphodiesterase</fullName>
            <shortName evidence="2">RNA 2',3'-CPDase</shortName>
            <ecNumber evidence="2">3.1.4.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_17560</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + H2O = a 3'-end 2'-phospho-ribonucleotide-RNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11828"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:17353"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:173113"/>
            <dbReference type="EC" id="3.1.4.58"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 2H phosphoesterase superfamily. ThpR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.58" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU18"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004113">
        <property type="term" value="F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008664">
        <property type="term" value="F:RNA 2',3'-cyclic 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01940">
        <property type="entry name" value="RNA_CPDase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019510">
        <property type="entry name" value="AKAP7-like_phosphoesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004175">
        <property type="entry name" value="RNA_CPDase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02258">
        <property type="entry name" value="2_5_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35561">
        <property type="entry name" value="RNA 2',3'-CYCLIC PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35561:SF1">
        <property type="entry name" value="RNA 2',3'-CYCLIC PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10469">
        <property type="entry name" value="AKAP7_NLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="LigT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="A-kinase anchor protein 7-like phosphoesterase" evidence="3">
        <location>
            <begin position="27"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HXTX 1" evidence="2">
        <location>
            <begin position="61"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HXTX 2" evidence="2">
        <location>
            <begin position="146"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="203" mass="23134" checksum="1B3D54DCACD8184D" modified="2018-07-18" version="1">MSEKRLVYEVTGNYYKDISKNVVMRTFVAIEISNNKVIDSIKNIQNKIKIDAKPIDVKNLHFTLQFLGEISDETSKKIIHALNTIEFSNFNINLIGIGAFPKPKFPRVVWIGTDENGGNMLTQLAKKVEKVLEPLGFSPDKPFKPHITVFRIKKKIGDITKELDSQKMIDFGIQEITSFKLKKSELTFSGPIYSDLEEIKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU21</accession>
    <name>A0A2S2KU21_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">sulfopyruvate decarboxylase</fullName>
            <ecNumber evidence="4">4.1.1.79</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_19340</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>3-sulfopyruvate + H(+) = sulfoacetaldehyde + CO2</text>
            <dbReference type="Rhea" id="RHEA:20948"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57940"/>
            <dbReference type="ChEBI" id="CHEBI:58246"/>
            <dbReference type="EC" id="4.1.1.79"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.79" evidence="4"/>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877746.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU21"/>
    <dbReference type="GeneID" id="76210164"/>
    <dbReference type="OrthoDB" id="77140at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050545">
        <property type="term" value="F:sulfopyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019295">
        <property type="term" value="P:coenzyme M biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051818">
        <property type="entry name" value="TPP_dependent_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42818:SF1">
        <property type="entry name" value="SULFOPYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42818">
        <property type="entry name" value="SULFOPYRUVATE DECARBOXYLASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0174">Coenzyme M biosynthesis</keyword>
    <keyword evidence="2" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="6">
        <location>
            <begin position="40"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH35143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="183" mass="20286" checksum="30740BF96240D9FE" modified="2018-07-18" version="1">MIRKEAVRLIAKEIGDQPIISSNGFMSRDVFETNEKENNFYMIGSMGLASSIGLGIALKKPKQKIHVFDGDGNILMNLGSLVTIGSLKPKNLVHFIFDNKSHESTGGQPTSSDKIELKKIAQAAKFKTFEIINKKQIKKIMSKVKKESGPIFVIVRIDKGGEKSIRVGIAPPNIKNRFMKSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU24</accession>
    <name>A0A2S2KU24_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ribose-phosphate pyrophosphokinase</fullName>
            <ecNumber evidence="1">2.7.6.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">NZNM25_17490</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the ribose-phosphate pyrophosphokinase family. Class III (archaeal) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="1"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_370684797.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU24"/>
    <dbReference type="GeneID" id="76208236"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000074">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000014">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF32">
        <property type="entry name" value="RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="15">
        <location>
            <begin position="6"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="14">
        <location>
            <begin position="153"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GBH34958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="292" mass="32040" checksum="353D76A166FDAD10" modified="2018-07-18" version="1">MLSNISIISGKSSENLARKLSKKIKANLVRSEIRVFADGESKITLNGKLSKKKSVVVQSIYPPVDTNLIQALSLISKAREVSSQVIAVIPYMGYARQDREFLPGEIVTMKVIGKLFKGAGASEVIAVDIHSLIGFKYFTIKTKNLTAIPDLVQYFKKLSLKNPLIVSPDQGGKKRAQEFAQEFGSEYIALEKKRDRKTGKVKIQTKNVNVLGRDLILVDDMISTGGSIVKATQFLKKQKCNRVYVACTHALLMNDARNKIMKAGVTKIVSANTIPGKTSIVDVSNTIAKAII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU28</accession>
    <name>A0A2S2KU28_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_19450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_264953692.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU28"/>
    <dbReference type="GeneID" id="76210175"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="116" mass="13984" checksum="12C5FC42F8718F44" modified="2018-07-18" version="1">MKERDPKYNISHKKMPNYSQHEKFVLSKPYDKWYIILKSSKEIGSIYLTKENEIGIFIKKNMQKKGIGFNVLKTLMEKNPRKRYLANINPMNKKSIRFFKNNGFKLIQYTFELEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU33</accession>
    <name>A0A2S2KU33_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Spore coat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_19430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DegT/DnrJ/EryC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU33"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00616">
        <property type="entry name" value="AHBA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000653">
        <property type="entry name" value="DegT/StrS_aminotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30244:SF34">
        <property type="entry name" value="DTDP-4-AMINO-4,6-DIDEOXYGALACTOSE TRANSAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30244">
        <property type="entry name" value="TRANSAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01041">
        <property type="entry name" value="DegT_DnrJ_EryC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000390">
        <property type="entry name" value="PLP_StrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0167">Capsid protein</keyword>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0946">Virion</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="374" mass="42598" checksum="E451B0B2CF46AF80" modified="2018-07-18" version="1">MNSILKDKNKRKKDMQKIKLFDPHVSNSEEIAVNKVLKSHFWASGSGVGLVKKFEEEFSKYIDTKDCVAVNSGTAALNLALSIFDVKNKEVILPSLSFVSTANAVIENGGIPKFADIDEKTLCIDTEKISKLISKKTRLILPVHFAGLAVNLNDISSICKKYGLDSVEDAAHASGTKYKNKKIGSHGDLVCFSFHPVKNLAMPTGGLIAINRNNHKKISKILKEKRWCGISNRKNTDYDVKEIGWNYYMNEFSAAIGLEQLKKLDRMNDFRRKVAKKYSDEIELEHKMPFDNNCSYHFYWIRVKNREKFRKKLFEKGIETGIHYKPIHTFSFYKSKIKLPVTEKISNEIVSLPTHPNLTGKDVEKIINTVNKSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU43</accession>
    <name>A0A2S2KU43_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Long-chain-fatty-acid--CoA ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">fadD</name>
        <name evidence="5" type="ORF">NZNM25_19530</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877763.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU43"/>
    <dbReference type="GeneID" id="76210183"/>
    <dbReference type="OrthoDB" id="35688at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031956">
        <property type="term" value="F:medium-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04433">
        <property type="entry name" value="AFD_class_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201">
        <property type="entry name" value="ACYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201:SF5">
        <property type="entry name" value="MEDIUM-CHAIN ACYL-COA LIGASE ACSF2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="8"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="4">
        <location>
            <begin position="397"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH35162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="479" mass="54053" checksum="2F6EAA226A3DA5DB" modified="2018-07-18" version="1">MNIINFLKKNVEKYPTKIAIIDEDASITFQKLYEEVQKFSTSISFLNEKDVISLISDNSISFVISYLGIINAGKLVHLIPPEIFESNLSTQLEASNSGAIICSKLNKKSFLENQSIKIPIYGIEEVLSNQEKISDIKKNNDLAYLIYTSGTTSDPKGVAVSHKMIDFTTKNIVKVLGYSDSDIDLLPLPLHHSFGLGCVHTSLYTGSTLVLLKNASNLEFMFETLKKFKVTTFAAIPATLTKILKFDRNLLESYFSNIRLIITNSTSIPKNTVQNFKHILVNGNLATYYGLTEASRSSFMIFDEKNDRDESVGKAAPKVEIKIVNDEKNESNLGEIWIKGDNVIKNYWRNYEADKNLIDGWLRTGDIGYFDEEKYLFLKGRNDDIINIGGEKVSPLEIEESVKEILGVDDVAAFGIMHEIFGQTIKINVVKAKDSDLSKSQILSHCIKNLEKYKIPSKIDFVERIPKTDYGKVKRFMLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU49</accession>
    <name>A0A2S2KU49_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_19020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877720.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU49"/>
    <dbReference type="GeneID" id="76210129"/>
    <dbReference type="OrthoDB" id="2830at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050595">
        <property type="entry name" value="Bact_response_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591:SF3">
        <property type="entry name" value="RESPONSE REGULATORY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591">
        <property type="entry name" value="STRESS RESPONSE REGULATOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="4"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="121" mass="13670" checksum="D0BE731BF36F7E98" modified="2018-07-18" version="1">MNNSVVVVDDNDDLLDSLTDFLQMKGFNILGCGHNGLEAVQLYEEYRPDVVLLDLSMPNYDGIYGLENIKKINPDAKVIILTGHYTEKNRQKIKPFNVSKIMEKPCSLIKLDQVLRSLSIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU62</accession>
    <name>A0A2S2KU62_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Universal stress protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">yjaB</name>
        <name evidence="3" type="ORF">NZNM25_18880</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877710.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU62"/>
    <dbReference type="GeneID" id="76210115"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF25">
        <property type="entry name" value="USPA DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="6"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="141" mass="15570" checksum="219001B7554753FD" modified="2018-07-18" version="1">MIKKQIKKILVALDGSQNSQRALSMAISLARNCDTKLVGMNVISNIPKKYHHLSYPEKPVLLAADNMMESAKILCAKNGILFERKIDFGDPGSKIVKFAESLNFDIIILGTRGMSGIKEKFFGSVSNYVVHKSSVPVMIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUC2</accession>
    <name>A0A2S2KUC2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2357 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_18660</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877691.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUC2"/>
    <dbReference type="GeneID" id="76210094"/>
    <dbReference type="OrthoDB" id="12014at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="519" mass="62240" checksum="507774341192C452" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUD6</accession>
    <name>A0A2S2KUD6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">STI1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_18590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUD6"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="201" mass="23820" checksum="3CF631B6593D64CE" modified="2018-07-18" version="1">MMNQLMQNMVANEKLMYHWHEMMQKNPSILMHTMSDWVSQMKEEPELMKNMLGPMTSEPQLREKMIESMKSHSKMENSLKMHSEWMDSVHHEMMNSGMHSTCSWCPNYQMHTSSPSMGFENSDRMMDVIHAIWVNSGMSNDMHRLMIQNPTHLAQMSYQLMDPMLDAIMDDEDLRQQMIKLMLENPEFMNSIRHENPETSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUE8</accession>
    <name>A0A2S2KUE8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_18960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUE8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF110">
        <property type="entry name" value="RHODANESE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="1">
        <location>
            <begin position="43"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="188" mass="22214" checksum="D0E486259F54735C" modified="2018-07-18" version="1">MNEFMSYNKEFWDKYADENEAKFNEEFAKYTRDLVTSLNCTSVLEIGCGTGIDLRLFPKSFQIHGVDLNDNALKMAKSKISFASFKKASITDLPFEDSSIDFVFTHQLLNYLDDDTLDKGIAEMYRVARKYIMNCEKFEETEKQIDENQKFRNVYKRWLNYNVRVVSNVDMHEDIEPDKSRFTLLRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUF7</accession>
    <name>A0A2S2KUF7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hydantoinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hyuA</name>
        <name evidence="3" type="ORF">NZNM25_19060</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877723.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUF7"/>
    <dbReference type="GeneID" id="76210133"/>
    <dbReference type="OrthoDB" id="8261at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF23">
        <property type="entry name" value="HYPOTHETICAL 5-OXOPROLINASE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase/oxoprolinase N-terminal" evidence="2">
        <location>
            <begin position="8"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase A/oxoprolinase" evidence="1">
        <location>
            <begin position="197"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="706" mass="76562" checksum="9DC43C2E9A41C43E" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNS3</accession>
    <name>A0A2S2KNS3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875978.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNS3"/>
    <dbReference type="GeneID" id="76209433"/>
    <dbReference type="OrthoDB" id="6023at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="83" mass="9836" checksum="1B2B20D0D7BA4158" modified="2018-07-18" version="1">MDFMLEEELIDLMTFCLQNPKSSEIPEKHKRITEIGHELYADGGLDALENFFFVLKNRIVEEIEKDPSPMRSLWNGLTDEWQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNW0</accession>
    <name>A0A2S2KNW0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875977.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNW0"/>
    <dbReference type="GeneID" id="76209431"/>
    <dbReference type="OrthoDB" id="11516at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="141" mass="15840" checksum="2FDD86F38CFD870F" modified="2018-07-18" version="1">MIVRILSALVMIMFLVTFSSAFALNELERATMNDPRLENAFGQPVDDNVNVNQQIQISADITNNQEKSQNFVYLVQIKNENDFVVSLGWISGQLTPNQKLNPSLSWTPNDSGEFVAEIFVWEGLVNHSALANYSEINIFVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP25</accession>
    <name>A0A2S2KP25_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Thiosulfate sulfurtransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_01830</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876051.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP25"/>
    <dbReference type="GeneID" id="76209514"/>
    <dbReference type="OrthoDB" id="9977at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01448">
        <property type="entry name" value="TST_Repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051126">
        <property type="entry name" value="Thiosulfate_sulfurtransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001307">
        <property type="entry name" value="Thiosulphate_STrfase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43855">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43855:SF1">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00380">
        <property type="entry name" value="RHODANESE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="19"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="149"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="265" mass="29927" checksum="F315153A0705CBDD" modified="2018-07-18" version="1">MTESGKITTDIDSLRSEIRDKSVRVIDVRREGDYKQDHIPTAVNLPLSNLLSDDSPERVLKLVNSMGIDDETPVVVYDDTFGALASRVAWTLEYLGHSDVTLLETTYSHWKSLGLENDSQTPEIQNKEHSMHLRPEILATSDYLESVKTRDDVILIDNRERLNFLEQHIPGAISLPYRTLASNDKILRSKDDMKRLLDNRGITGDSEIITYCGSVGTLSGLAYYALKSAGLGNAKLYVRSFKEWKSLQKPTEKQEDASYWDLSAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP28</accession>
    <name>A0A2S2KP28_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dimethylhistidine N-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_02340</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876097.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP28"/>
    <dbReference type="GeneID" id="76209568"/>
    <dbReference type="OrthoDB" id="120600at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035094">
        <property type="entry name" value="EgtD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051128">
        <property type="entry name" value="EgtD_Methyltrsf_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019257">
        <property type="entry name" value="MeTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017804">
        <property type="entry name" value="MeTrfase_EgtD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03438">
        <property type="entry name" value="egtD_ergothio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43397">
        <property type="entry name" value="ERGOTHIONEINE BIOSYNTHESIS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43397:SF1">
        <property type="entry name" value="ERGOTHIONEINE BIOSYNTHESIS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10017">
        <property type="entry name" value="Methyltransf_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018005">
        <property type="entry name" value="UCP018005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine-specific methyltransferase SAM-dependent" evidence="3">
        <location>
            <begin position="35"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="340" mass="39612" checksum="8D1F36ACABFCF634" modified="2018-07-18" version="1">MSNTLQKNLNYKKYVIDNRLQYFKPHASRIEKTFAEEISFSLNRHCKFISPKFFYDKKGSDLFEKICMLPEYYPTKTEITILKKLQNELPSYLDKNFRVVELGSGASVKTRIILDIFSQIYDVVEYFPIDISEILAESSESLLKDYDHLHITGIIDTYEGGLEFLKNYDEKKNLILFLGSSFGNFSPVDGHEFLQKINSTMKKGDLFLIGLDLVKDRQILESAYDDSQGITAEFNLNVLSRINDELDADFNLNNFSHHSLYNEHDQRIEMYLKSLVNQSVVISKSDLLLNLEKDELIHTEYSHKYKLSDIHKLLDDVGFDIKHTWLDDKNYFSLTLASKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP48</accession>
    <name>A0A2S2KP48_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH hxlR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_02000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876067.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP48"/>
    <dbReference type="GeneID" id="76209532"/>
    <dbReference type="OrthoDB" id="376044at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002577">
        <property type="entry name" value="HTH_HxlR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01638">
        <property type="entry name" value="HxlR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51118">
        <property type="entry name" value="HTH_HXLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH hxlR-type" evidence="1">
        <location>
            <begin position="4"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="99" mass="11382" checksum="550F0C2FCA253ABE" modified="2018-07-18" version="1">MDNMDFMIFLRMLSKKGFLETLWHITEKKQVHYNELQKYLMKKNIVSSQASVTIILNGLTNLGLLDRIVVDGKPPRTSYSVNKLGKSVLTQLNNLKNTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP50</accession>
    <name>A0A2S2KP50_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Translocase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_00560</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875936.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP50"/>
    <dbReference type="GeneID" id="76209385"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.3310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003369">
        <property type="entry name" value="TatA/B/E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42982">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42982:SF1">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02416">
        <property type="entry name" value="TatA_B_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="68" mass="7594" checksum="087878B7A9ED1557" modified="2018-07-18" version="1">MLGMGLANFIAGQEWIFIIIIAVVFIFGAKKIPELAKTFGKAKGEFEKGKIEGEKELKDFKDKETKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP74</accession>
    <name>A0A2S2KP74_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_00590</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP74"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="53" mass="6093" checksum="774535BF546A46CA" modified="2018-07-18" version="1">MFVLDMLYQFELQMAGVILLTAMAVGGISLWLKRRKLNKELQDSTEADNTQNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP87</accession>
    <name>A0A2S2KP87_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_01950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_264953648.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP87"/>
    <dbReference type="GeneID" id="76209527"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049886">
        <property type="entry name" value="CFI_box_CTERM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041770">
        <property type="entry name" value="CFI_box_CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="316"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="346"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="374" mass="41424" checksum="9974973EBF9EEFBF" modified="2018-07-18" version="1">MSDKSQESYAVILGTVIVIIFTVSLTENLQFADAISVSADKLIYDIEVGDTLLLYWSVRNTDPYPIDIEFYATGPGSELLVFEQFGHLDVKERKSFEILASIPADYEDNVEYRPVLHVLQRSQDPDSEESVDGASVAKVNVVMKTVPIIRIGENPVYNPPVIENLEKPESDPALEKAKSPEVIEKAETIEEKLARIQAFNQANTQKDTSQTTTIENPVVQDDNSRYEPEPKMDPEPVIDTIVDKNEMVNGGGCLIATAAYGTEMAPQVQFLREIRDNTVMSTSFGAAFMTGFNTLYYSFSPTIADLERENPVFQEAVRAFITPMISTLTIMMLAEDGSEIEVLGLGISVITLNLGMYIVAPAVVGFTINKRLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPC1</accession>
    <name>A0A2S2KPC1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Thymidylate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876254.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPC1"/>
    <dbReference type="GeneID" id="76209492"/>
    <dbReference type="OrthoDB" id="8310at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050797">
        <property type="term" value="F:thymidylate synthase (FAD) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20175">
        <property type="entry name" value="ThyX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.170">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003669">
        <property type="entry name" value="Thymidylate_synthase_ThyX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036098">
        <property type="entry name" value="Thymidylate_synthase_ThyX_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34934">
        <property type="entry name" value="FLAVIN-DEPENDENT THYMIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34934:SF1">
        <property type="entry name" value="FLAVIN-DEPENDENT THYMIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02511">
        <property type="entry name" value="Thy1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69796">
        <property type="entry name" value="Thymidylate synthase-complementing protein Thy1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51331">
        <property type="entry name" value="THYX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="538" mass="63395" checksum="640A8837DDF88918" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPD7</accession>
    <name>A0A2S2KPD7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01460</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876015.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPD7"/>
    <dbReference type="GeneID" id="76209476"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="307" mass="34205" checksum="052D0C691E8021E7" modified="2018-07-18" version="1">MNISIPTDSVILKKLALSKQIFQRGLIHSQSETNVDKLMAVILFDLSTETVLNAIITSIDSSKTPSDGFPSLLNQVESMLTSATLGGIPDRANILRVHSIRNDAQHDARYPNNSEVSDCQTYTRDFLKKIVEQVWGLNFEQISLADLIQNEKIKNILKDADLALERKEIQTAINESVMGLEKTLSIVGGSLVGGSLTYLFDQIVTTSSFDGMKGNDEITRSFKKIQETLRFVSLGLDYSKYLKFKSITGQPLFTLGNDKPKDFFDQKKDPALNDAEFVVAFAIDSVLLIEEKVGDIDKPFGKDPVWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPF5</accession>
    <name>A0A2S2KPF5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mut7-C RNAse domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_01660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876035.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPF5"/>
    <dbReference type="GeneID" id="76209496"/>
    <dbReference type="OrthoDB" id="1266at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002782">
        <property type="entry name" value="Mut7-C_RNAse_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39081">
        <property type="entry name" value="MUT7-C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39081:SF1">
        <property type="entry name" value="MUT7-C RNASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01927">
        <property type="entry name" value="Mut7-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mut7-C RNAse" evidence="1">
        <location>
            <begin position="3"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="156" mass="18470" checksum="E8BB41EDF9B17599" modified="2018-07-18" version="1">MLFLVDAMLGNIARKLRLFGYDTEYFSDIDDHELLEKAKNENRTIISKDECLIRRAKKKGIQFIGITTEDEIEQFKEILKTTNLEINKISGDLARCTKCNFQTFQIKKSEIQNKIPKRILDYHDKFWKCGVCDKIYWEGTHIEKLQEFVQKLKCLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPG8</accession>
    <name>A0A2S2KPG8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_02120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146195965.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPG8"/>
    <dbReference type="GeneID" id="76209546"/>
    <dbReference type="OrthoDB" id="375341at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="176" mass="20059" checksum="6061163634D1E6E9" modified="2018-07-18" version="1">METLSKIAIYMGMGIACSIFVMFTISIMPHVVSQIENNWEDVLPGKSDEELKKMFYETDSYKEFNKKYPDNGEYFRSYGDGRGSLEVTAMNFESYNTLRLELEYDKRTDSVREDITCNNQIDNRNLHIRGTLAAQFIEKISCLEGSGIIDAPSNLIDEFGNPVPINISPRVTVDID</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPH6</accession>
    <name>A0A2S2KPH6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_02950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876150.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPH6"/>
    <dbReference type="GeneID" id="76209632"/>
    <dbReference type="OrthoDB" id="378401at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="11"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="63" mass="7641" checksum="0D330FFE82095632" modified="2018-07-18" version="1">MTQSEYDQKDLNNYEKLQNEYKKLLTEYDELKSDNPQNTELDEKVKELTEKHKEIQDLSSKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPH8</accession>
    <name>A0A2S2KPH8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_02220</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876089.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPH8"/>
    <dbReference type="GeneID" id="76209556"/>
    <dbReference type="OrthoDB" id="65817at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="10"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="74"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="144" mass="15990" checksum="FE073AB1C8DB2A52" modified="2018-07-18" version="1">MGSTFVNQFMNKNVLTADKSTSLQEAAQSMTKLNVGCVIVTENAKPIGIITERDFVTKVAAEGRPLFTEISEVMSSPLITIEPGETVWEASEMMREELIHKLPVKENDKIVGIITTTDIVKISSVGSDSEMRRICDQILLRMRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPI3</accession>
    <name>A0A2S2KPI3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleotidyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_03570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876205.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPI3"/>
    <dbReference type="GeneID" id="76209698"/>
    <dbReference type="OrthoDB" id="15372at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="233" mass="26617" checksum="95E3E8EC3E89218D" modified="2018-07-18" version="1">MKAVILAGGLGTRLQPYTTFLPKPMLPLGEKPILEHLVDWTRKNGVKSVVLCVSYLRKTIEDYFEDGKRFGVNIEYAISDKPLATAGQLKTAEEFIDDTFVCIYGDSIFNFSLRNMIKQHQKKKSFVTMSLNEYKTNLPYGVIETTKNGKVLSWNEKPEIKANVNMGCYVMEPEILKFIPKNKPFGMDDVIKKAMDRKKLVNSFLTKNGFTDIGNKTSYKKAYQEYIQKLGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPJ2</accession>
    <name>A0A2S2KPJ2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF1512 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_02780</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876134.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPJ2"/>
    <dbReference type="GeneID" id="76209615"/>
    <dbReference type="OrthoDB" id="15121at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009995">
        <property type="entry name" value="DUF1512"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056461">
        <property type="entry name" value="DUF1512_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056460">
        <property type="entry name" value="DUF1512_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07431">
        <property type="entry name" value="DUF1512"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23542">
        <property type="entry name" value="DUF1512_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016495">
        <property type="entry name" value="UCP016495"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1512" evidence="2">
        <location>
            <begin position="24"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1512" evidence="3">
        <location>
            <begin position="204"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="376" mass="42073" checksum="2507D163169784FD" modified="2018-07-18" version="1">MSLVNFTSFDFDQIFAMGDDTNPLMMLIWILPIFFFVFYGQRIQLYVTSGEIKKGIAKLDGFRKESRDELINYVKITLKPVDDPTTKIDRFIDYFTIMPVDMDPNGIVDKVRHIVRSREDYTREHVRSISPQMNDIELTRVQTLLEIASSLQMIYKVINHMYLTAKKQNNYPLILPLQMILPFIMEQSEAMKEAIPVFKKGQPVGDGIGPMVVGMMMLKHQKETVAFQTVLAKSEFNGRKLFLLKAEGPGSTVGRPADGLEKIISENKIDAIIMIDAALKMEGEDSASVAQGFGAAIGGMGIEKFEIEAIATSKNIPIFSIVVKQSVKEAITLMTKDIAYKADDVCSQVHEMIQENTMEGQSIVIIGVGNTVGVPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPL2</accession>
    <name>A0A2S2KPL2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Galactose-1-phosphate uridylyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_03600</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPL2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008108">
        <property type="term" value="F:UDP-glucose:hexose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053177">
        <property type="entry name" value="ADP-glucose_phosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001937">
        <property type="entry name" value="GalP_UDPtransf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005849">
        <property type="entry name" value="GalP_Utransf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42763">
        <property type="entry name" value="ADP-GLUCOSE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42763:SF2">
        <property type="entry name" value="ADP-GLUCOSE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01087">
        <property type="entry name" value="GalP_UDP_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000808">
        <property type="entry name" value="GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2 6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Galactose-1-phosphate uridyl transferase N-terminal" evidence="5">
        <location>
            <begin position="98"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-UMP-histidine intermediate" evidence="4">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="355" mass="40182" checksum="AC20C6FB3F8CF0A9" modified="2018-07-18" version="1">MSSLSKRFNRHLLSVTNMGDMRKDYVSERFMIVTKKDDKIVDPKKSPFAPGNESMTNPSVLSLVAKDGMLQRLQDNEDEYVEGWAIRVFESKNPIVSIDTENSYSDRPFYSEPAYGYHYIVVASPKEKDTFATIDPEQWSNVLVVVQDRLRWLYTQKGVTYVSIYADHGDLAGSTNPHPHLNLITFSTIPPVIESEAEASHKILNEKGVCPVCQTVNEEISGPRQVLQTEGFIAFCPWSPSYPYEFWISPKKHTTSFSKITQKEINDLSLILRATLGGLSKTVKNVSYNLVFHLSPEKKNSRQIHWHIEVYPITKSWSGLERGYGIFLNDVSPEQAAEKLGASCRKELANLVGIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPN4</accession>
    <name>A0A2S2KPN4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fe-S cluster assembly protein SufB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_04040</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron-sulfur cluster assembly SufBD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPN4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055346">
        <property type="entry name" value="Fe-S_cluster_assembly_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010231">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045595">
        <property type="entry name" value="SufBD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01980">
        <property type="entry name" value="sufB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508">
        <property type="entry name" value="FES CLUSTER ASSEMBLY PROTEIN SUF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508:SF1">
        <property type="entry name" value="UPF0051 PROTEIN ABCI8, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19295">
        <property type="entry name" value="SufBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="Stabilizer of iron transporter SufD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD N-terminal" evidence="3">
        <location>
            <begin position="136"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD core" evidence="2">
        <location>
            <begin position="206"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00043967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="469" mass="52774" checksum="A4EDAE0ADFB3EB9E" modified="2018-07-18" version="1">MMGMATENLDMDYSKYDFKDSTELYVHLSKKGLSKETVISISKMKGEPEWMLDFRLRSYEIFMKKPMPTWGGDLSVIDFQNIYYYAKASDKVEKNWDDVPDSVKKTFDKLGIPEAEKKFLAGVGAQYESEVVYHSLREDLAKQGVLFLDTDAALKEYPEIFKKYFGKIIPPEDNKFAALNSAVWSGGSFIYIPPGVKVDMPLQAYFRINAENIGQFERTLIIADEGSEVHYIEGCTAPVYSSESLHSAVVELVAHKDAKLRYTTIQNWSSDVYNLVTKRAYAYEGATVEWIDGNIGSKLTMKYPGIYLMGERAYGETLSIAFAGKGQHQDTGAKMVHLAPNTTSKITSKSVSRLDGRSTYRGLLNVAKGATNVKATVRCDALLLDDTSKTDTYPYMEINQEDATITHEATVGKIGDEQIFYLMTRGFTEEEALSLIVNGFMEPFTKELPMEYAVELNRLIKLEMDDSVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPT7</accession>
    <name>A0A2S2KPT7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">3-isopropylmalate dehydratase</fullName>
            <ecNumber evidence="6">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Alpha-IPM isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">leuD</name>
        <name evidence="14" type="ORF">NZNM25_04730</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the LeuD family. LeuD type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876342.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPT7"/>
    <dbReference type="GeneID" id="76209825"/>
    <dbReference type="OrthoDB" id="6505at2157"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009316">
        <property type="term" value="C:3-isopropylmalate dehydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01577">
        <property type="entry name" value="IPMI_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000003">
        <property type="entry name" value="3-isopropylmalate dehydratase small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01031">
        <property type="entry name" value="LeuD_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004431">
        <property type="entry name" value="3-IsopropMal_deHydase_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033940">
        <property type="entry name" value="IPMI_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050075">
        <property type="entry name" value="LeuD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00171">
        <property type="entry name" value="leuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002458">
        <property type="entry name" value="PRK01641.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43345:SF5">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43345">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT 2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="13">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GBH33682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="194" mass="22000" checksum="69670D7DEEC04176" modified="2018-07-18" version="1">MEPFQNISGIVTPLDKVNVDTDQIVPKQFLKLVQKSGFGKFLFFNWRYDENGNKKSDFVLNESKYDGSKILVTGDNFGCGSSREHAVWALKDYGFSVIIAPSFADIFFSNCFKNGILPISLDEKTVDSLQNEKDPISVDLKNQTIKTSSQEIHFDIDSHLKKILLEGLDDIAQTFQYEDKITEFEKNSKIPSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPU8</accession>
    <name>A0A2S2KPU8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphate import ATP-binding protein PstB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pstB</name>
        <name evidence="5" type="ORF">NZNM25_04150</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPU8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03260">
        <property type="entry name" value="ABC_PstB_phosphate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005670">
        <property type="entry name" value="PstB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00972">
        <property type="entry name" value="3a0107s01c2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423:SF1">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="30"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="277" mass="31126" checksum="B7D78F6D4D37267A" modified="2018-07-18" version="1">MTALSIKSANTEEQIPSSSSSVVDSEKYKMIAENVTISYDGIPAVKNVTMKFKEKSVTALIGPSGCGKTTFLRCLNRMHDMTKNAKVEGKVMIDNIDLYDKSIDPIYHRRKVGMVFQKPNPFPTMSIYDNVTAGLKLNGVRDKKILNEIVEDSLKMAYLWDEVKNDLNKSAIELSGGQQQRLCIARALAIQPEVLLMDEPASALDPIATQKIEETITELKKEYTIIIVTHNMQQAVRVSDYTGFMYLGDLIEFRETKKLFTDPKNDLTAKYVKGHFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPW1</accession>
    <name>A0A2S2KPW1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4760 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_04970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876362.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPW1"/>
    <dbReference type="OrthoDB" id="4367at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="20465" checksum="235553B93F83FBA1" modified="2018-07-18" version="1">MPNSNKWRMAVDLSTLPVIISSITATIFAITLIITWKNIKENTRATHSQLLRSFHEDLLNRLNKNTILKTTEDCQRYANDYLNTVDEIAYLELNRKIPSHIATYLKRFFAYGLMIVDWYNEMVGEDFRETAKKNWPNYIEYCKKYDIVSNPDDKLPKVMLDYNKLRENESQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPW6</accession>
    <name>A0A2S2KPW6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04350</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146195973.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPW6"/>
    <dbReference type="GeneID" id="76209784"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="99" mass="11741" checksum="17D2024455F2BA14" modified="2018-07-18" version="1">MKSMSISPTEDHVMKRDYKIKLSGDDFVLVGMNYNYFMIPRLAYPKDNLEKLKQQILDDQKMTDELKTLWDDGMQDKSLEKISQILEKYFGKKAEQQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPX4</accession>
    <name>A0A2S2KPX4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_05310</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829038.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPX4"/>
    <dbReference type="GeneID" id="76209882"/>
    <dbReference type="OrthoDB" id="6762at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154">
        <property type="entry name" value="LEUCINE-RESPONSIVE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR AZLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="143" mass="16049" checksum="2D49B6DBC72D7425" modified="2018-07-18" version="1">MKLLFELTRDGSISVPTLSKKLGINASVLYSRIKRLMKKKLIKKFTVVIDDSLLGIGVKASVGINRDPKQKDAIHKLFMEIPEVVSISEVTGRFDIIIKVYAKDLEALHTIVIEKIGKVPGIQNSETFVELQKTDKDPVYLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ00</accession>
    <name>A0A2S2KQ00_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_05370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146195977.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ00"/>
    <dbReference type="GeneID" id="76209889"/>
    <dbReference type="OrthoDB" id="2906at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="96" mass="11277" checksum="59E9D4FF2AE4FC77" modified="2018-07-18" version="1">MKFIFDIVNWLSVHSDIREEIKNLEDNILRLEDNIAEFLSMKYDEGVKKLLHSLESDLKYLSILANGAPIDKNEDRKIMDFLRTHYARLQKLSVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ08</accession>
    <name>A0A2S2KQ08_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Histidine kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_05340</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876395.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ08"/>
    <dbReference type="OrthoDB" id="8127at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3090">
        <property type="entry name" value="ORF SCO4226, nickel-binding ferredoxin-like monomer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042557">
        <property type="entry name" value="SCO4226"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065:SF10">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065">
        <property type="entry name" value="SENSOR HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="97"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="298" mass="33654" checksum="51672C6842C20758" modified="2018-07-18" version="1">MPIFLDMHELGDYTKEQLVAGLEEDADEFGVLVHQMLFNEKENILHCICTAPDVAAIEKHHEKFNVKCKKIIPINQIKTDKVIKEEKLKTIGELSSRLSHDIRNPLTILQTSLDVLRSNNPETYEKNISRFNTMYDAIKRIDHQISDVLGFLGTRKLNFTETDLDDILNSSIEGMKIPTQIVVEMPQNKISLNCDFESMRTVFSNIILNAIQAMNDTGKIIIKALKKEDSISISFENSGPAIPEKIISKIFDTLFTTKQEGTGLGLVSCKNIVEQHGGKIEIKNNPTTFTVILPQKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ20</accession>
    <name>A0A2S2KQ20_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rps7</name>
        <name evidence="10" type="ORF">NZNM25_05630</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876419.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ20"/>
    <dbReference type="GeneID" id="76209915"/>
    <dbReference type="OrthoDB" id="45346at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14867">
        <property type="entry name" value="uS7_Eukaryote"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00480_A">
        <property type="entry name" value="Ribosomal_uS7_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_uS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026018">
        <property type="entry name" value="Ribosomal_uS7_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_uS7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_uS7_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005716">
        <property type="entry name" value="Ribosomal_uS7_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003106">
        <property type="entry name" value="PRK04027.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01028">
        <property type="entry name" value="uS7_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="Ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS7" evidence="9">
        <location>
            <begin position="33"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH33772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="199" mass="22312" checksum="A2C38A9A088119D3" modified="2018-07-18" version="1">MAETKNLLLFRKWDLSNIEIKDPGLKTAISLRKQILPYTYGRSALKRFNKADVNIVERLINKTMHFGKKYAKNTGRMTGKKSKLLNTVKTAFDIIALKTGENPVEVLVRAIENSAPNEDTTRIVYGGTVYHVSVDVAPIRRVDLALKFISDAIKEATFSNPKPIEEHMAEHLILAAANDPNAPSVKKKNELERIAQASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ32</accession>
    <name>A0A2S2KQ32_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_05050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ32"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="182" mass="21168" checksum="21616342794C456B" modified="2018-07-18" version="1">MVKKQLSTEQKKKTETMSFRLDSQILDKLRAESESQGVSLNILANKIFARYVDWGMFEPKVGMVPIAKPIVTALFQKLSEQETVDLAKQIGKSIVSDIATFMKGSLDLDSFVSWFITRMKISDFELNHTIQNSTHSYIIKHDLGYNWSLYHKTVLELIFNDAFEKRIDFKIKDRMMELTFEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ35</accession>
    <name>A0A2S2KQ35_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PEFG-CTERM sorting domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_05730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876428.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ35"/>
    <dbReference type="GeneID" id="76209925"/>
    <dbReference type="OrthoDB" id="11870at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027560">
        <property type="entry name" value="PEFG-CTERM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04296">
        <property type="entry name" value="PEFG-CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="28925" checksum="A00BE77CDDAB90F4" modified="2018-07-18" version="1">MKAHLTVFTLSAILIVSIGMAPAFGQIQNSIVVTTDKSSYSEGETIIVTGEVRDLYSGTPVSLIVKNDNAIVSLAQLTIGADKKFRTELTAGGTMKTAGTYTIEVTYGTQNRIATTTFEFGGSTSTPPKNTGVTDTTVSIEGSSDLIGYTITGGKLLGIMPDVEAKSLIVSIDATSDGSLTLTIPRSILDATMNGEDDEFFVLIDGEEVNFEETTSATERILTIAFPVGAEEIEIIGTFVIPEFGTIAAMILAVAIISIIAVSAKSRLSIMPRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ36</accession>
    <name>A0A2S2KQ36_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transport permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_04220</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876300.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ36"/>
    <dbReference type="GeneID" id="76209770"/>
    <dbReference type="OrthoDB" id="147058at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047817">
        <property type="entry name" value="ABC2_TM_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051784">
        <property type="entry name" value="Nod_factor_ABC_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43229">
        <property type="entry name" value="NODULATION PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43229:SF2">
        <property type="entry name" value="NODULATION PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006648">
        <property type="entry name" value="DrrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00164">
        <property type="entry name" value="ABC2TRNSPORT"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="229"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-2" evidence="6">
        <location>
            <begin position="24"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="261" mass="28485" checksum="88DF6EE2E70A5196" modified="2018-07-18" version="1">MNTLMYDTYTIFWRELKRYKKSRSGVLIRLIQPAIWIIVIGNTFSGTQPLIQSVGFEGEYIEFMAPGVIILTAIFTSIFGGVNTLWDRRYGFMNKALTSPISRSAIALGKMSAISLIAAMQASLILGIALAIGVTFPNPIMIAPIMAIVILFSLGFSGISVIVAATAKSQETFWGVINFLGMPLFMLSPALFPLELLPNWLAMIAKLNPVTYTVLLVRELMTGISEGGISIALSLGVISVFVLVMVGLASYVFTREVNKPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ93</accession>
    <name>A0A2S2KQ93_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Orotate phosphoribosyltransferase</fullName>
            <shortName evidence="7">OPRT</shortName>
            <shortName evidence="7">OPRTase</shortName>
            <ecNumber evidence="2 7">2.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pyrE</name>
        <name evidence="9" type="ORF">NZNM25_06240</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10380"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="2 7"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876473.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ93"/>
    <dbReference type="GeneID" id="76209979"/>
    <dbReference type="OrthoDB" id="9089at2157"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000058">
        <property type="entry name" value="Orotidine-5-phosphate decarboxylase/orotate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004467">
        <property type="entry name" value="Or_phspho_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00336">
        <property type="entry name" value="pyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278">
        <property type="entry name" value="OROTATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278:SF9">
        <property type="entry name" value="URIDINE 5'-MONOPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 7" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="8">
        <location>
            <begin position="57"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <begin position="121"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="201" mass="22461" checksum="F707EE3129B10D38" modified="2018-07-18" version="1">MEFVKEFATFLYQKGIIKFGDFTLASGKKSSYYVDLRLVPSYPHEFRKMVKYLENEIAQKIGLENFDSFVSVPTGGLVITSALAIETVKPLIYVRSKPKDYGTSKAVEGKIHDGMKVIMIDDVATTGGSVVNAIKSLKEVNISIKDAYVIVNRMEGADEALADLEVNMHSILNILQITETLHKQNLVDESILEKVKKQINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQB5</accession>
    <name>A0A2S2KQB5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription initiation factor IIB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tfb_3</name>
        <name evidence="7" type="ORF">NZNM25_04360</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TFIIB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876310.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQB5"/>
    <dbReference type="GeneID" id="76209785"/>
    <dbReference type="OrthoDB" id="7429at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="125"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="219"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="309" mass="34282" checksum="E1619106D2253188" modified="2018-07-18" version="1">MPILQKITKENVFCNYCHASKLVTDEVTGEIACSNCGYVILENTEERGREHRLISDTGDTARTGPGLTLKRHDQGLNTVIGIQNKDAVGKPLSSKTTQMFDRLRKWDSRSQTKNSADRNLRFALQEFDKVQSKLGLSDTVIERASLFYRKAIERNLIRGRTVKSIAAACLYASCRDLGHNRTLIEIANQFLINRKEIARAYRILFRELGFVVSIADPIKSITKIASKMNVSEKTIRKAVQILDAAQDARITAGKNPEIIAATVIYAASIITGESMSQTAIAEAAGTSTVSIRNRIQEFKAKLGLFSTIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQE5</accession>
    <name>A0A2S2KQE5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_05680</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876424.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQE5"/>
    <dbReference type="GeneID" id="76209920"/>
    <dbReference type="OrthoDB" id="12300at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="390" mass="42961" checksum="B1EFA2F1A4E6B811" modified="2018-07-18" version="1">MLLFLISLILLPAFGATIPDYDNPYSPIFTDKSSYTWTDKVKMTIIAPSWNTDNDLIDSIGDTKDHPIKISTREHSLEPYKFTETDVNSGIFTAEVILTGFFHDADGDGDFDTNPRTMGNGPTSGFLEVERDSAITISFEFADGVVLTESAPVTWNVGTIQFMQEIFLTTDSVNIRMVDPDMNLNPEALDHIPIQVISDSDVAGIEVDGIETSESSGMFVATVSLTQSSTSGNRLYAIPGDKISAKYDDYTLPKPYSISDNLSLQTFAIVDSAVPPIERIDHLSITFSDSLGNPLQSFTENHQIQIVGTIANPNEFDQKFVYLFQVKNEQNIVESISWVQGELSSAQSLDVSQSWTPKTSGNYKIETFVWNSIAEPTGLAPIMSTLITVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQF9</accession>
    <name>A0A2S2KQF9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Histidine--tRNA ligase</fullName>
            <ecNumber evidence="2 10">6.1.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">NZNM25_06870</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 15">
            <text>tRNA(His) + L-histidine + ATP = L-histidyl-tRNA(His) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="2 10"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQF9"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000065">
        <property type="entry name" value="Histidine--tRNA ligase protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015807">
        <property type="entry name" value="His-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00442">
        <property type="entry name" value="hisS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707:SF1">
        <property type="entry name" value="HISTIDINE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707">
        <property type="entry name" value="HISTIDYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="12">
        <location>
            <begin position="51"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="70"/>
            <end position="72"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="262"/>
            <end position="263"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="NCBIfam" id="TIGR00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001549-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH33896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="421" mass="47676" checksum="6190D3757E6CEE14" modified="2018-07-18" version="1">MKDFEGAENANIEHVRYHFKQLSNLYGFSFMDPSPIELLSTLETKSGPGIRDEIYYFKDKGDREVALRFDFTMGLTRYAASQKSMKLPAKLSSFGGVFRYDEPQKGRYRYFHQWDIEIYGKPSIESEAEIIELTSRLFDSLLLREITIDINHRNLVESYINKIFNSNDPELVADILRAVDKIAKKSKDEILKEFQAKGYQTEKIEKILEFSQIKGTISEVEKIFDTTQLGSWNELKQLFDSLENRGVSNVRINFGIVRGLDYYSGTVFEVFDKNSTLGALAGGGRYDTLTKAFGRDDIGATGVAGGVERIILTMQEQKIIPKILQKRVAVLYINEEMQKVAYSIASLLRLNNIPTDIDLAGRNLKKQMDIASNAQLSIIVGPDELEEGNVVLKDMVNGTEGTISLEKLTEDPKSILNLEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQH8</accession>
    <name>A0A2S2KQH8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Snf7 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04990</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876364.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQH8"/>
    <dbReference type="GeneID" id="76209851"/>
    <dbReference type="OrthoDB" id="3538at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="23791" checksum="A525CB10B5CD4CD7" modified="2018-07-18" version="1">MPTFQDNWNKPPTVGLNQHILNTVKKEPPLKPRIQQSVGKLNQTISKMDFMFKKLQEKDKKIFKRIVESQQRHDKYTSKVLANELIEIRKNEHVLTNIKLALEQIQIRLSTMNELGDAMVSIGPAIAVLRSMGPALSKFMPQAGAEFEAMSGLLGEITSDSFGGDFELSDTSNEETDAILKEAAVIAGNRVGEKFPSSPATTYSQMESRLNQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQI9</accession>
    <name>A0A2S2KQI9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Flagellar protein FlaI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_07100</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876577.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQI9"/>
    <dbReference type="GeneID" id="76208965"/>
    <dbReference type="OrthoDB" id="33500at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01130">
        <property type="entry name" value="VirB11-like_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050921">
        <property type="entry name" value="T4SS_GSP_E_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486:SF14">
        <property type="entry name" value="FLAGELLA ACCESSORY PROTEIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486">
        <property type="entry name" value="TWITCHING MOTILITY PROTEIN PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bacterial type II secretion system protein E" evidence="2">
        <location>
            <begin position="230"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="556" mass="62096" checksum="2072F2482D364087" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQJ7</accession>
    <name>A0A2S2KQJ7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MFS transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_07510</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQJ7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17370">
        <property type="entry name" value="MFS_MJ1317_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23518">
        <property type="entry name" value="C-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23518:SF2">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="303"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="1"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="360" mass="39322" checksum="7CC2EAD74D4AA893" modified="2018-07-18" version="1">MFIIDELGAGKAVLGIVEGMGESVGYGTRTISGTISDKIGKRKSLILLGYGLSTLAKPFFAISTSVAHIVGIRVTDRIGKGIRTSPRDALLSDSIEPTNIGKAFGLHRTMDQAGAIIGPLIAFGLLYYFEVRDIFWFSLIPGGIALFILARHVSERKITPKNQSVFSNFRDVLHGKFLLYLIIIMVFSIGAFNFSFVLVESSELGLEKNFVPLVYVVINVAHTLIGMPSGILSDKIGAEKVLLISMGLFLITSSIGYFETEFVLVGFAMAAIFGLYHGMTITAQRTVVSRYVSEQLRGTAFGVYYLFVGASFLVANITFGFLWDVFDSQAAFGYSITTSIIGMVLLLIFIYKNKQKTSDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQP5</accession>
    <name>A0A2S2KQP5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Succinate--CoA ligase [ADP-forming] subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">sucC</name>
        <name evidence="8" type="ORF">NZNM25_07740</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876631.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQP5"/>
    <dbReference type="GeneID" id="76208901"/>
    <dbReference type="OrthoDB" id="146449at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042709">
        <property type="term" value="C:succinate-CoA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006104">
        <property type="term" value="P:succinyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000055">
        <property type="entry name" value="Succinate-CoA ligase, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013650">
        <property type="entry name" value="ATP-grasp_succ-CoA_synth-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005809">
        <property type="entry name" value="Succ_CoA_ligase-like_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815:SF10">
        <property type="entry name" value="SUCCINATE--COA LIGASE [GDP-FORMING] SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815">
        <property type="entry name" value="SUCCINYL-COA SYNTHETASE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08442">
        <property type="entry name" value="ATP-grasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001554">
        <property type="entry name" value="SucCS_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="7">
        <location>
            <begin position="9"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="368" mass="40338" checksum="929D69EA34D44E8E" modified="2018-07-18" version="1">MQLLEFQAKELFREYGINLLPSISSTNIEEGRKHAKELGYPFVIKIQVPVGGRGKAGGIQKCQNDDEFELKYPQVMDLTIKGEKARAILLEKMADIKKELYLSLFLNRSKRCYTIIASSEGGVEIESVKNQIIKEVGLGDVSDEIAREVAKEIGLQGNTAEQFVDTLKKLSKLTIEKEAELVEINPLAIMQDDSIMALDGKFVTDDNSNFRHPELQKYQEKTEIEEQAEKSGFSLVELEGDIAVVGNGAGLVMSTLDMLSDNGGKPACFLDVGGGATTESVYEALTLISKLSRVKGILVNLYGGIVKTTVVAEAFLKAYEDNLIDLPVFARLKGTESDKAKEMLQGSRTNIFDSVEGAINAAVMGIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQP7</accession>
    <name>A0A2S2KQP7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Small nuclear ribonucleoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_05690</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876425.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQP7"/>
    <dbReference type="GeneID" id="76209921"/>
    <dbReference type="OrthoDB" id="371816at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044641">
        <property type="entry name" value="Lsm7/SmG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553:SF5">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="3">
        <location>
            <begin position="4"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="76" mass="8672" checksum="7873255CF98E0E9E" modified="2018-07-18" version="1">MADEITNLMNNNKDKVVLLRLRNARTVQGVLKDFDIHMNLTLDDAEDVTEEKHEKIGKVLLRGDNILLVSLPEEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQQ4</accession>
    <name>A0A2S2KQQ4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chromosome partitioning protein ParB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">parB</name>
        <name evidence="2" type="ORF">NZNM25_06520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876494.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQQ4"/>
    <dbReference type="GeneID" id="76210010"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1530.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050336">
        <property type="entry name" value="Chromosome_partition/occlusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004437">
        <property type="entry name" value="ParB/RepB/Spo0J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00180">
        <property type="entry name" value="parB_part"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33375">
        <property type="entry name" value="CHROMOSOME-PARTITIONING PROTEIN PARB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33375:SF1">
        <property type="entry name" value="CHROMOSOME-PARTITIONING PROTEIN PARB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="ParB/Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB-like N-terminal" evidence="1">
        <location>
            <begin position="14"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="273" mass="31181" checksum="72CFF08C4F24B026" modified="2018-07-18" version="1">MLRRYKPKISYRLREIPIKQIKIWKDAQARKLDRENISELAKSIKNEGLLNPPLVQKENKNTYLLMSGQRRLAAMKRLGAKKIPVHLLSKKTEYDLENAKAASVVENIHRNDMNHREIADACKFLTEQVGKSSAAKSMGMSLATLNKYLGFAGVPEKLKLLVPSLVSRDEITRLYFIVPNVAKAEKIVQRISGLDHILRKKYLIALSHTPKSSHQKLLKRAKSLRVKQNISLKLSKTNAKKLAIESNKKDIQPDELAGKIISDYLKRKKSRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQQ9</accession>
    <name>A0A2S2KQQ9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PEFG-CTERM sorting domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_05760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876430.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQQ9"/>
    <dbReference type="GeneID" id="76209928"/>
    <dbReference type="OrthoDB" id="11241at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027560">
        <property type="entry name" value="PEFG-CTERM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04296">
        <property type="entry name" value="PEFG-CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="266" mass="29262" checksum="908589B97F2F1073" modified="2018-07-18" version="1">MEFRIFYILATLLIISTSTVFAQESIITIQTDDNHYDEGDTIVISGNVNTVIGDTPVLIQIVQEGAIIEIAQIIVAQDGTFTKTIIAEGGVWKKGGEYTIRAFYQEHIAESSFSFTPKSEASETTTNFEVDAGSRGTFDVEYTIRGGTVKNMVVDSEIFALIVQVDSIDEGLITLNLPREFIGAEKQDGKDDTFIILIDGIEVAYQESVVGSESRVITINFEQGDSDIEIIGTYVVPEFSTIAVMILIVGIMTTIVMTQKKFQLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQT9</accession>
    <name>A0A2S2KQT9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQT9"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="399"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="423" mass="47300" checksum="F10B67B1C7E6B99A" modified="2018-07-18" version="1">MRILSNRYTAFSIKNIPILGLENPLMSKFYNFPIIGNTMQKWFCLFVIFASVGVATNYAYSQEMSLATFQETAQVIIDKSISQNVTASITLQSTSIQEIKIPAELEQRIRENGRISVVTVTNQNQCVLGVNNESCILINVKRNPDDKNFAQIQNSTLQIAGQFIDEINEVFDTKAKLHSTFIHSSDDASVALETSGVISGRGTISAVYTMPMEDTDSMYEKISAILIPKVIRDSGGFYDVARNLSTQENSKMTFSIISSDNKSLLQLRLSVNYPNAAPEINEISPLEFLKTDELKRSDYFSLGFYPLNSIFQVVILSDEETKVSDVKGDITPTQVIGDERIPTDITKKGWVFDPQEGERIQGKYIFGKDTAITKKELEFSLGGEEFKSKKPEKLEVDESVIVVIIITIVAIAASVFYLKGYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQU4</accession>
    <name>A0A2S2KQU4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07750</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876632.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQU4"/>
    <dbReference type="GeneID" id="76208900"/>
    <dbReference type="OrthoDB" id="7214at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="109" mass="12834" checksum="11F913E8F4399621" modified="2018-07-18" version="1">MVKQISLDAWQIQQLSDLLEKGSSIVEKTNRPIVLYRQTLEEEEESYEEIVCTLTKGYVIEQMVTSGGVLVPSFHQQFVFTIEEYPEELLRKSKDRFLEMIDFLEEQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQU7</accession>
    <name>A0A2S2KQU7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876668.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQU7"/>
    <dbReference type="GeneID" id="76208853"/>
    <dbReference type="OrthoDB" id="2079at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="83" mass="9039" checksum="F542B9433D3259EB" modified="2018-07-18" version="1">MAKIKVRSGRGTRIVEVDDSANKWTGSDFKKSQEARKEASKDKYVSVGRGTGTIKFGDIPTTTTQSTKGMWVSSGRGTGKRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQW6</accession>
    <name>A0A2S2KQW6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Lipoyl-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_06390</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876484.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQW6"/>
    <dbReference type="GeneID" id="76209996"/>
    <dbReference type="OrthoDB" id="6555at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000003">
        <property type="entry name" value="Acetyl-CoA carboxylase biotin carboxyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050709">
        <property type="entry name" value="Biotin_Carboxyl_Carrier/Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45266">
        <property type="entry name" value="OXALOACETATE DECARBOXYLASE ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45266:SF3">
        <property type="entry name" value="OXALOACETATE DECARBOXYLASE ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0092">Biotin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="2">
        <location>
            <begin position="94"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="170" mass="18684" checksum="B396CEE344C6DF47" modified="2018-07-18" version="1">MDYKITDIEKSFEGKILQNLGNNDYVIKVNDNEHQLKIISMNSSGIEFILDQKYHNAKYLEQSTNEMNIVVDNVPIIINRHSHFDEVVYKHSGGAGAGNAQLSLKSQIPGKVVSIAVSEGDSVKKGDVICTLESMKMQVSVKAHKDGVVKSLKIKETATVAKGDPIADIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQZ2</accession>
    <name>A0A2S2KQZ2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_07420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876603.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQZ2"/>
    <dbReference type="GeneID" id="76208934"/>
    <dbReference type="OrthoDB" id="4921at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="139" mass="16730" checksum="E0E3C595F501C473" modified="2018-07-18" version="1">MNEINEEQENATQKVLELLEEWGSESRFIWFREIHRITEIDKGLLRNILNELKKSKEVKDMHGTNNRIFFCLKKYYVKCRDVEFRFKGKEIMLRDGSKTKIIQGSTNATERTRKRLEKQKNKRRAKAFTKAKNKRPHKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR04</accession>
    <name>A0A2S2KR04_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146195985.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR04"/>
    <dbReference type="GeneID" id="76208787"/>
    <dbReference type="OrthoDB" id="387075at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="104" mass="11864" checksum="C54F127070A13C6F" modified="2018-07-18" version="1">MKTRFLIIISLVIMGTIPFGTFPKSFSACIFDEDAGRYLEPCRGLPMPPPLSLKQQIADNVSMFEIKCPNPEHVLTQRPNSSLACVFPETQEKLNWQIVYYIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR08</accession>
    <name>A0A2S2KR08_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08840</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876722.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR08"/>
    <dbReference type="GeneID" id="76208786"/>
    <dbReference type="OrthoDB" id="3294at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="362" mass="41129" checksum="9FEF2013EA885F03" modified="2018-07-18" version="1">MKTRFLTLIVGLVIASSISFVVLWNALEECDEQCELEKRRIEAGRPNIGVLELPKKQEPVEDITVMRPNSMEFFYYPNPEDTENRDVFQKFILIRLPEALGGTVDDVSAFRAYSALSVGVHCQIKYWPDDGRQRMEDPCWGSMYRPTDGAMMYPYPVMNDSPLGLPYLDLSIDENGSLYVEPPVWTMQENGVIGVGRIMSMQEIRQGSQILIDSYKKTNPNHPMIPVDFAGLVLAEIHPDHNNVNARYTDFASSSYQNISFEVRNVSAQDQKSFLNVAKPNSEFWQINDTIIRIGGSAMDKNSSLPESFRDYNVEFILDGFTFVITGPDLNSIKTSIVSNYFPENNYDDLFLVSSTVMENEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR26</accession>
    <name>A0A2S2KR26_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glucose/Sorbosone dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_06990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876533.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR26"/>
    <dbReference type="GeneID" id="76210059"/>
    <dbReference type="OrthoDB" id="6744at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012938">
        <property type="entry name" value="Glc/Sorbosone_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH_b-prop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19328">
        <property type="entry name" value="HEDGEHOG-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19328:SF13">
        <property type="entry name" value="HIPL1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07995">
        <property type="entry name" value="GSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="Soluble quinoprotein glucose dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose/Sorbosone dehydrogenase" evidence="1">
        <location>
            <begin position="35"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="491" mass="55271" checksum="0F3ECE6869FE0B48" modified="2018-07-18" version="1">MKEIIFAVILFSIPVSFAQEYPEIGVKVETVAENLTIPWSIDWLPDGTILFTERNGHLRVIQNGELLEEPLLSLGVGGVEGGMLGVAVDPDFEENNFIYLYYTYNEFLSTINKLVRFQLVDGKLTEDKMLLDGIPGGPFHDGGRIQFGPDGKLYITTGEAGDPKLAQDLNSLGGKILRINSDGTIPEDNPWENSSIYSIGHRNPQGIDWDEFGNLVATEHGPSGWRGVAHDEINLIFPGMNYGWPDIIGDETADGLQNPILHTGDDTWAPSGSEFYYGDKIPQWTGKYFVATLRGSHLHMIDFDLQEKKVLSHEKLFQDEFGRLRDVQTGPDGFLYILTSNQDGRGFPNANDDKILRIIPINSINNFEECVAFGNPVMESYPRQCRTEDGKHFVEVISEIPDWVRKTAKWWSLNQIADEEYASGLQYLIEKNIIIVPAGTLAEGSSEQQLPSWLRKDAGWWSQGLISDEEYFKSIQWMINNNFIKTELGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR76</accession>
    <name>A0A2S2KR76_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hemerythrin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876704.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR76"/>
    <dbReference type="GeneID" id="76208813"/>
    <dbReference type="OrthoDB" id="10087at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.520">
        <property type="entry name" value="nmb1532 protein domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012312">
        <property type="entry name" value="Hemerythrin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39966">
        <property type="entry name" value="BLL2471 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39966:SF1">
        <property type="entry name" value="HEMERYTHRIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01814">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hemerythrin-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="181" mass="21496" checksum="8FD2D8A2BEF0C5D0" modified="2018-07-18" version="1">MSATNQLRTDHDQVKRLEKIVFKCSEELKKGTDIPFLDLSKIMIVISEFIDTIHHSREEDSYFPCVSNHDSLKDEIHRFLVEHEFGRNVARLISRHLKLWKEGSDEREPVSRYLNAYGVFLKDHLHKENKFFDTAEATVLTKEEETNMYEEYKSVFAIVKKVEDMIKEIDYLESRPWNLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR87</accession>
    <name>A0A2S2KR87_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH arsR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_09730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR87"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38600">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38600:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="47"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="131" mass="15709" checksum="FFD798C4AA57CC81" modified="2018-07-18" version="1">MFYYMSNQLLEFKEIIRSKHVSNTRKPDKQTRKLLLYLFTSTRGGFTRLRIIVHLLERPYNTHQLAQTLDLDYKAVQHHMKILEKNNMVSKIGEKYGAIFHLSNFLELNIHTLEEAIDKLDRKMNQKKIYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR97</accession>
    <name>A0A2S2KR97_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glyoxalase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_09910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876818.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR97"/>
    <dbReference type="GeneID" id="76208676"/>
    <dbReference type="OrthoDB" id="144489at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07246">
        <property type="entry name" value="VOC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34109">
        <property type="entry name" value="BNAUNNG04460D PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34109:SF1">
        <property type="entry name" value="VOC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="8"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="156" mass="17166" checksum="92F92679E79B20F7" modified="2018-07-18" version="1">MTYACPKGFSSVTPHLVIKDCANALDFYKKALGAQEIYRSKMPDGRIMHAMIQIGNSFVMMADEFPDMGAVGPNTLGGTSTSLHIYTDDADKLFKQATDAGATPIMPIADMFWGDRYGQIQDPYGHRWAIATHTKDVSPEEMEKAAKEMFSKGNSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR98</accession>
    <name>A0A2S2KR98_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein eL31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl31e</name>
        <name evidence="7" type="ORF">NZNM25_09250</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876760.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR98"/>
    <dbReference type="OrthoDB" id="10127at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00410">
        <property type="entry name" value="Ribosomal_eL31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000054">
        <property type="entry name" value="Ribosomal_eL31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023621">
        <property type="entry name" value="Ribosomal_eL31_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002258">
        <property type="entry name" value="PRK01192.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956:SF0">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01198">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01380">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54575">
        <property type="entry name" value="Ribosomal protein L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="78"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="88"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="167" mass="19151" checksum="473F5993FD687042" modified="2018-07-18" version="1">MSQELERVYTINLGKALLSQSQHRAVRAINMIKEFARHHMKVEDIKIEEELAHQIWARGIRSPPRKVRVRMSKTDEGFVLVSQYTDEVESKVTPEKETKKVSDKAEPTKEAPKKEPSDVESKVTPEKETKKVSDKAEPTKEAPKKEPTKEAPKKEPTKEAPKKEPKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRD7</accession>
    <name>A0A2S2KRD7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07990</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRD7"/>
    <dbReference type="OrthoDB" id="9999at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="133" mass="14907" checksum="B119B3FD51028B73" modified="2018-07-18" version="1">MASIIAVLSIGTIPVDISAETNQNRVTVTPINEEISLQKTVATMNIPEDNKLPWGFVKGAPSEYVERYPVIIQFYSGEDPVHFAQVDVKGDGSFEYKFRVRNMDSSTGEFVNIFEGEYTVKIFRVIPNSNDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRF5</accession>
    <name>A0A2S2KRF5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_09120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876748.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRF5"/>
    <dbReference type="GeneID" id="76208758"/>
    <dbReference type="OrthoDB" id="12229at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="184" mass="20315" checksum="C53CD07A35AFC457" modified="2018-07-18" version="1">MKTLFLLSLVLLFAISPAYGQSLSDATGLVTRLDVKTGGHTFEVETVSNFNIPDFNFDKDEKKLSLFINSGLERNLGELLIPRALLGGNFTFYLNEQPYFPKVHSNEKISFVTLNFTGSGENKLEIFGTTYLDGLTAKDEITPKELPPLQKGETLPDSLGWLVLVGFLAVVSAIVIVKIKKQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRH2</accession>
    <name>A0A2S2KRH2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_09580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_263970066.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRH2"/>
    <dbReference type="GeneID" id="76208711"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="44" mass="5525" checksum="ADDBACE203F04906" modified="2018-07-18" version="1">MSENNEETEKKWKKLEQDEMREEDHQIELMEGYEQDEEYEQSND</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRI3</accession>
    <name>A0A2S2KRI3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper-containing nitrite reductase</fullName>
            <ecNumber evidence="3">1.7.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">NZNM25_08560</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>nitric oxide + Fe(III)-[cytochrome c] + H2O = Fe(II)-[cytochrome c] + nitrite + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16301"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.7.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>Cu(+)</name>
            <dbReference type="ChEBI" id="CHEBI:49552"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotrimer.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.7.2.1" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRI3"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050421">
        <property type="term" value="F:nitrite reductase (NO-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd11024">
        <property type="entry name" value="CuRO_1_2DMCO_NIR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001287">
        <property type="entry name" value="NO2-reductase_Cu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF394">
        <property type="entry name" value="FI03373P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00695">
        <property type="entry name" value="CUNO2RDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 10" id="KW-0186">Copper</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="5"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="13">
        <location>
            <begin position="54"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="12">
        <location>
            <begin position="193"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="type 2 copper site" evidence="10">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601287-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GBH34065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="399" mass="45109" checksum="D0744BFD17C4B276" modified="2018-07-18" version="1">MDRTIVMLVGVISLGVTLGIFFLDAELPLQSFADKNSVHNSPITRTYTVIAEDTTLEIAPGTRVEAWTYNGTIPGPTLRATEGDRVIINFINNGKLPHTMHFHGDHNEKNDGVFQEVLPGESYTYDFTAEPAGLFMYHCHVMPVSEHIRNGLYGAFIVDPKEGLEPAREYVLVKGEYDLEDQETWTPDYVFFNGYADQYWNNPLPVKTGEKVRLYYVDMGAIAAFGFHIHGTIFDTIISGIWENEPIKTQTWEVSPGNAAIFEATWKEPGRYLFHLHGVPEEKGTMAYFDVRDASSDAVDGVDVARTKSVDMWEWQKQIAINLQQPDTNGKITKTSDSSLDDIFGEPVNICFKINQYALPNTVVMDDSVYRKAKDLQFKFTKLDQSLIKDLEYTVFILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRI8</accession>
    <name>A0A2S2KRI8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator AsnC/Lrp ligand binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876696.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRI8"/>
    <dbReference type="GeneID" id="76208822"/>
    <dbReference type="OrthoDB" id="8136at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF6">
        <property type="entry name" value="REGULATORY PROTEIN ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="1">
        <location>
            <begin position="16"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="1">
        <location>
            <begin position="103"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="187" mass="20894" checksum="6E8DE92D6C959D52" modified="2018-07-18" version="1">MAKAYILMNCDLGSEIDVISSLKNIEGIKEAHGTLGLYDIVAKIELGSEKKIREVVTGIIRKMPKIHSTLTLTRSESEGLFQTSEKLLGAILGQNIAQAYVVIHCKNGEEYTILKNICHIPEVKEADVVFGLYDVICKIQASDNKTLENVITKAIRALHDIESSMTLNIINEQEQRTEILDRHMMVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRK1</accession>
    <name>A0A2S2KRK1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Endonuclease GajA/Old nuclease/RecF-like AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_10700</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876927.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRK1"/>
    <dbReference type="GeneID" id="76208589"/>
    <dbReference type="OrthoDB" id="25344at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041685">
        <property type="entry name" value="AAA_GajA/Old/RecF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051396">
        <property type="entry name" value="Bact_Antivir_Def_Nuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43581:SF3">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43581">
        <property type="entry name" value="ATP/GTP PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13175">
        <property type="entry name" value="AAA_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease GajA/Old nuclease/RecF-like AAA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="48"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="697" mass="80940" checksum="93805CF4A9AB49A0" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRP8</accession>
    <name>A0A2S2KRP8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Histidine kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_09090</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRP8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065:SF10">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065">
        <property type="entry name" value="SENSOR HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Histidine kinase" evidence="9">
        <location>
            <begin position="169"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="19"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="372" mass="42038" checksum="8A0DE9708280D2AB" modified="2018-07-18" version="1">MLILYTSPMRKYLVRKNFDENNTDPMNAPNRTVFYERERKSYEHNTQKSSSESEEEIETTASLIQTKKMDLEEGQPLLNSEAKRIFQDSLTALNAADKQRNDIIYMQLESEKKISQELNRKLHANLSKIASAEVELIKRKNQLEDELHEKTKQLVNSERMAAIGELSGRLAHELRTPLTVIKGSVGVLKLRKGTEIDNFVMERLALMEESVSRMNHQVEQVLNYVQKIPLDKKDVSLKQVLQKSLSLIQRNPNITILTPKNDITFNCDSIKMEIIFGNLLLNAVQAIGTESGQIAIELGETEDSIKITVQDSGSGVPDEVGEKIFEPLVSLRQDGTGLGLSSVKNVVEQHNGKISFTNNPTKFTVVFPKVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRQ3</accession>
    <name>A0A2S2KRQ3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phenylalanine--tRNA ligase</fullName>
            <ecNumber evidence="4">6.1.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">NZNM25_11430</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="4"/>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876984.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRQ3"/>
    <dbReference type="GeneID" id="76208513"/>
    <dbReference type="OrthoDB" id="10073at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.40.10:FF:000003">
        <property type="entry name" value="Phenylalanine--tRNA ligase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="entry name" value="Phenylalanyl-trna Synthetase, Chain B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004531">
        <property type="entry name" value="Phe-tRNA-synth_IIc_bsu_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00471">
        <property type="entry name" value="pheT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947:SF0">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE BETA CHAIN AND LEUCINE-RICH REPEAT-CONTAINING PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B5" evidence="13">
        <location>
            <begin position="269"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GBH34352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="543" mass="59447" checksum="94B54ABF179E28DA" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRV6</accession>
    <name>A0A2S2KRV6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Geranylgeranyl reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_11700</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877006.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRV6"/>
    <dbReference type="GeneID" id="76208482"/>
    <dbReference type="OrthoDB" id="6062at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000546">
        <property type="entry name" value="Geranylgeranyl hydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050407">
        <property type="entry name" value="Geranylgeranyl_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054715">
        <property type="entry name" value="GGR_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685:SF18">
        <property type="entry name" value="DIGERANYLGERANYLGLYCEROPHOSPHOLIPID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685">
        <property type="entry name" value="GERANYLGERANYL DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12831">
        <property type="entry name" value="FAD_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22578">
        <property type="entry name" value="GGR_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Digeranylgeranylglycerophospholipid reductase catalytic" evidence="8">
        <location>
            <begin position="210"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="485" mass="54250" checksum="65855AD2499D86D4" modified="2018-07-18" version="1">MVDYDVIIAGGGLAGTITAQAISHYSNQNAKILVVDRNTEFLPGRKSLAGWVCGDACSKEAVDYMSDRIKVKWTKPEIEHDVKGVMAFSPDKETAIPFDGAGYMLNRQKLPEIQNERCRKMGIDFQYEINLTGLVYDGQQVVGVQGIDNKTKQPFKKTAKIVIDATGVTSMLRNGLQNSTKVEKRIDRRDLESTGRYIMYFEPGQKDLSEFDPNYCIIHLDQDIAPGGYGWVFPKAENKVNIGLGVEKSLLDKRNKRLGKKDNVESLMKEYLARNIAIKNPKLSEDPQDINNSSGVFQVSVRRQNDCMVSGGYMMVGDSAWMPKPIDAGGIGPALIAGTILGNNVAQALQANDVSEASLWQYNLDYIKEYGYKTAGLELFRRLVQQMTNEQISYGMKHFLGNLDVEAISKGEHPDFTGLGKIGMIIRGAMNKTVADGLRYTSKQNQWLVEHYHNYPKDPSGFDEWNKTLHQRLDEAFTKVEAFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRY5</accession>
    <name>A0A2S2KRY5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12310</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877078.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRY5"/>
    <dbReference type="GeneID" id="76209273"/>
    <dbReference type="OrthoDB" id="15210at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036113">
        <property type="entry name" value="Asp/Glu-ADT_sf_sub_c"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141000">
        <property type="entry name" value="Glu-tRNAGln amidotransferase C subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="89" mass="10439" checksum="5F78D1C58C90D267" modified="2018-07-18" version="1">MGIEEEIEHVSKLMKIDIDDHKEHVEKVRMMISYFDILDSAGVESEEISVHEISLSNLRDDEYIPFNEKLIEQLKHYKGTYVRAPKMSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS00</accession>
    <name>A0A2S2KS00_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TATA-box-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Box A-binding protein</fullName>
            <shortName evidence="7">BAP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">TATA sequence-binding protein</fullName>
            <shortName evidence="7">TBP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">TATA-box factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tbp</name>
        <name evidence="9" type="ORF">NZNM25_11750</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7 8">General factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Binds specifically to the TATA box promoter element which lies close to the position of transcription initiation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the TBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048115071.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS00"/>
    <dbReference type="GeneID" id="24819621"/>
    <dbReference type="OrthoDB" id="350539at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000007">
        <property type="entry name" value="TATA-box-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000010">
        <property type="entry name" value="TATA-box-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00408">
        <property type="entry name" value="TATA_bind_prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030491">
        <property type="entry name" value="TBP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00686">
        <property type="entry name" value="TIFACTORIID"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00351">
        <property type="entry name" value="TFIID"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="repeat" description="1" evidence="7">
        <location>
            <begin position="10"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="repeat" description="2" evidence="7">
        <location>
            <begin position="101"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="187" mass="20882" checksum="866AC0B38C2CE125" modified="2018-07-18" version="1">MPQTKPIVSVENVVASASVDQKIDLNDITEKFPDTEYHPEQFPGLVFRLKSPRTATLIFRTGKMVCTGAKSEEMAIKAVNTVVQKLRKGKIKIKKDAVITVQNIVAAINLGGKIHLEKAARTLPRSMYEPEQFPGLIHRMLDPKTVILLFASGKLVCTGAKKESDVYRSVHNLHALLEEKNLMIYDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS38</accession>
    <name>A0A2S2KS38_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_12150</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877317.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS38"/>
    <dbReference type="GeneID" id="76209289"/>
    <dbReference type="OrthoDB" id="66915at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="60"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="94"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="128"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="196"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="264"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="371" mass="42029" checksum="DBBFD2E2661BDBE0" modified="2018-07-18" version="1">MIKKYLFVLFCIGFFIPIVCAQTVDEATLLENTKKFLMDKQYDDAIIELDKILAVQPENVNALIEKGHVRLIQGKYVKAFQNFEKALQFEPKNILALSGQGTAQYHLKQYEDALDSFNSVLKVDKDNTVALVGSGNILLKEQQYDNAILFFNIVLEREPNNISAMIAKASALAGINQYDKSLELLDNILQLDSDNILALNEKGQVYLEIEKYALARDVFTDLMEIDENDIYALLGMAELNFLENKNIKAEQLYDKVLLLDPDNVEGLLGKANVLESRGQYDDALRYFDEALEVDPDNLEASNGKGSILEHEVNASLDLILIITIIGSVTSVFSFIIVMIKIKENKKLQERVLIADEQIEDMVDKFMKRQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS43</accession>
    <name>A0A2S2KS43_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877011.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS43"/>
    <dbReference type="GeneID" id="76208474"/>
    <dbReference type="OrthoDB" id="11979at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="230" mass="25349" checksum="D326F381BFA19FDC" modified="2018-07-18" version="1">MNDSLLDDVKALLDKDFGDDRILKQIYRACENNEVISNYERTYVQKLAEKHLGKRPEILRTPTIEQKPITPDVAIPQTQSIQKMQTFQQEPPRKSYLKSKNSKIMLGITGLALIIIIGVAASFSFDMSPKVETPTTTVPVTLSIQSDLSSYNKKDLISISGVSNTSGTVNLSIENPNNELVWAEQVSLKSNGAYSTLAIAGGQGWENSGTYTIKVDNGKETKSITFAFKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS48</accession>
    <name>A0A2S2KS48_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Intracellular proteinase inhibitor BsuPI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_10690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876926.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS48"/>
    <dbReference type="GeneID" id="76208590"/>
    <dbReference type="OrthoDB" id="11885at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="16712" checksum="E77C02AFFCC86296" modified="2018-07-18" version="1">MVSKKIFIVFVLPVIFSIVFGSAVMSDILQKPDRELHLLPMSTGGLSIQNSSIEIIGLSKQYSTSEPVEIQIKVRDSSFDCGDLYVTIYSSTNDVVTQGGYFEQCFEKGSNMIPTDDEFSKSIETPGSYEIVAEMVSKQLKSASARGSFTVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS68</accession>
    <name>A0A2S2KS68_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Archaetidylinositol phosphate synthase</fullName>
            <shortName evidence="2">AIP synthase</shortName>
            <ecNumber evidence="2">2.7.8.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_13030</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the formation of archaetidylinositol phosphate (AIP) from CDP-archaeol (CDP-ArOH or CDP-2,3-bis-(O-phytanyl)-sn-glycerol) and 1L-myo-inositol 1-phosphate (IP or 1D-myo-inositol 3-phosphate). AIP is a precursor of archaetidyl-myo-inositol (AI), an ether-type inositol phospholipid ubiquitously distributed in archaea membranes and essential for glycolipid biosynthesis in archaea.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>CDP-2,3-bis-O-(phytanyl)-sn-glycerol + 1D-myo-inositol 3-phosphate = saturated 1-archaetidyl-1D-myo-inositol 3-phosphate + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36823"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:74004"/>
            <dbReference type="ChEBI" id="CHEBI:74006"/>
            <dbReference type="EC" id="2.7.8.39"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 Mg(2+) or Mn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.39" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877129.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS68"/>
    <dbReference type="GeneID" id="76209196"/>
    <dbReference type="OrthoDB" id="9904at2157"/>
    <dbReference type="UniPathway" id="UPA00085"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02242">
        <property type="entry name" value="AIP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044270">
        <property type="entry name" value="AIP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2 3" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="150"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="190" mass="20307" checksum="00F570396041C0DA" modified="2018-07-18" version="1">MLNNLRETLRPTLEKIGKGFASTGLSPNFWTVIGLAFALISAIVYGIGIEYGLILGGVLLLVSGFFDMVDGQVARVTGKTSKKGSYLDSMFDKISEVAIFLGILIGGYAEPYLVLLAITLSLLVSYARAKSDAINIKLQGIGIGERAERLLVISIVGIIGFMDIAVVIVVIIAGITLIQRMIVTAKNIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS70</accession>
    <name>A0A2S2KS70_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF424 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_10890</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876940.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS70"/>
    <dbReference type="GeneID" id="76208570"/>
    <dbReference type="OrthoDB" id="18015at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1860.10">
        <property type="entry name" value="uncharacterized conserved protein from methanopyrus kandleri domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007355">
        <property type="entry name" value="DUF424"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04242">
        <property type="entry name" value="DUF424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="98" mass="11029" checksum="AA55BDCC5916B060" modified="2018-07-18" version="1">MQFSVRVTEYQKNMMLNICDAELLGKKIIQDELNIHISESYYGQRFVEKDEAINLLKQSSIINMVGKETISLSINLGVGSENGVKTISGVPFLIVFNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS79</accession>
    <name>A0A2S2KS79_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Iron transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_10990</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876948.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS79"/>
    <dbReference type="GeneID" id="76208559"/>
    <dbReference type="OrthoDB" id="6721at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3880.10">
        <property type="entry name" value="Fe(II) trafficking protein YggX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007457">
        <property type="entry name" value="Fe_traffick_prot_YggX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036766">
        <property type="entry name" value="Fe_traffick_prot_YggX_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04362">
        <property type="entry name" value="Iron_traffic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111148">
        <property type="entry name" value="YggX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="93" mass="10876" checksum="F666F0358A712656" modified="2018-07-18" version="1">MSRICTKCKNPIPDTEQLGVVAEKYPTCNKCWAEWKEYQIMVMNEMKLDMSMLDHRKLLKKHEKIFVGVLSPEGEVIDYTNEDNRKPDEPTNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS90</accession>
    <name>A0A2S2KS90_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13070</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877133.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS90"/>
    <dbReference type="GeneID" id="76209192"/>
    <dbReference type="OrthoDB" id="9885at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="152" mass="18002" checksum="4FF790972272530F" modified="2018-07-18" version="1">MITQNSTKQESTNETPKVSVCDIMKNNTSMMIHKMETEVPTYMQLYSDVYTKYLEMFDNLFGTCYISEKQFFDKLGLDQSTLKTINNFWKTMSENYNTQIDISTNFLKSYVQMHISAIESYEEYMKILMDSYSKTLFEINKFTKFSNVNKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSA7</accession>
    <name>A0A2S2KSA7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA polymerase</fullName>
            <ecNumber evidence="7">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_13510</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="7"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877168.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSA7"/>
    <dbReference type="GeneID" id="76209148"/>
    <dbReference type="OrthoDB" id="323192at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05783">
        <property type="entry name" value="DNA_polB_B1_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.690:FF:000011">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000208">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1390">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050240">
        <property type="entry name" value="DNA_pol_type-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004417">
        <property type="entry name" value="PRK05761.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322:SF20">
        <property type="entry name" value="DNA POLYMERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322">
        <property type="entry name" value="DNA POLYMERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="9">
        <location>
            <begin position="118"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="8">
        <location>
            <begin position="426"/>
            <end position="817"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="852" mass="98281" checksum="FDA0498A954F5317" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSB4</accession>
    <name>A0A2S2KSB4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aspartate--tRNA(Asp/Asn) ligase</fullName>
            <ecNumber evidence="11">6.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Aspartyl-tRNA synthetase</fullName>
            <shortName evidence="11">AspRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Non-discriminating aspartyl-tRNA synthetase</fullName>
            <shortName evidence="11">ND-AspRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">aspS</name>
        <name evidence="13" type="ORF">NZNM25_12320</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Asx) + L-aspartate + ATP = L-aspartyl-tRNA(Asx) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18349"/>
            <dbReference type="Rhea" id="RHEA-COMP:9710"/>
            <dbReference type="Rhea" id="RHEA-COMP:9711"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 3 Mg(2+) cations per subunit. The strongest magnesium site (Mg1) is bound to the beta- and gamma-phosphates of ATP and four water molecules complete its coordination sphere.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.23" evidence="11"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877079.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSB4"/>
    <dbReference type="GeneID" id="76209272"/>
    <dbReference type="OrthoDB" id="5908at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050560">
        <property type="term" value="F:aspartate-tRNA(Asn) ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000038">
        <property type="entry name" value="Aspartate--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000324">
        <property type="entry name" value="Aspartate--tRNA(Asp/Asn) ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02075">
        <property type="entry name" value="Asp_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004523">
        <property type="entry name" value="Asp-tRNA_synthase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00458">
        <property type="entry name" value="aspS_nondisc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003483">
        <property type="entry name" value="PRK05159.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450:SF1">
        <property type="entry name" value="ASPARTATE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450">
        <property type="entry name" value="ASPARTYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="12">
        <location>
            <begin position="145"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Aspartate" evidence="11">
        <location>
            <begin position="200"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="221"/>
            <end position="223"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="229"/>
            <end position="231"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="366"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="407"/>
            <end position="410"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for tRNA non-discrimination" evidence="11">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH34441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="436" mass="49335" checksum="CBFAD5A9E60258BB" modified="2018-07-18" version="1">MIETELGTLRRSHYSDEILPSMDGSQVTIMGWVVTIRGHGNISFATIRDKNGDISVVAKKGDCSDEIRATISSLKSHSSIAVTGNVKSSEKAPSGFEIIPTELRVFSNVEKIPPFEPTAKTVKNIDTRLEVRPIDLRRISLQHIFKTRSLVLKSIRDYFNEQKFIEINTPKMIATATEGGAALFPIFYYNKEAFLAQSPQLYKEQLTMSFEKVFEIAPIFRAEPSRTNRHLAEAISIDLEEAFVDYNDVMNRIEEIIKISIMSVNKYVDENAGVEFTIPSMPETIPRYSYDELVDRMQKAGAKTEWGDDLYPSNLKKIGLDGFYFIKDWPLAPKPFYVKDSKSNPKVSESFDLMFGDLELSSGSTRIEKREELAERMKNKGMNTDAFEYHLGAFDYGVPPHAGCGIGLERLIMALTGTENIRDVTFYPRDVDRLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSB8</accession>
    <name>A0A2S2KSB8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11390</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876982.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSB8"/>
    <dbReference type="GeneID" id="76208517"/>
    <dbReference type="OrthoDB" id="5769at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="210" mass="23463" checksum="462C73013E5F533B" modified="2018-07-18" version="1">MVNKGFPKFGMSQAGAFVAALKNYNLPDFILVLIAKECKSDLLERGRIDDRLQSMNDDALELLHKVFVGCEEDNAGKYAQYRFYAYVSSMYHKCEVLVNETIPGASGKNHKIPVAVKNNGMYISVAYNKATGNPVNKKETIRFYDMVDDIKKGDHGTMLADAIYGSSVGFRGDALVELENLSKSRTNDPENKLDFKTANFENNIYSVTKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSE8</accession>
    <name>A0A2S2KSE8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator TrmB N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_13350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877156.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSE8"/>
    <dbReference type="GeneID" id="76209163"/>
    <dbReference type="OrthoDB" id="30795at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051797">
        <property type="entry name" value="TrmB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="1">
        <location>
            <begin position="14"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="285" mass="32023" checksum="771CF700F038DE45" modified="2018-07-18" version="1">MSYQDQVSRIATELEEILDLDDLEAKVYLNLLRAGPITASALAKELDIDRARMYRTVDRLVNRNIISTTLSSPKLCIASDPHDALKIALSKKEDEVNKIKKLGEAIIDKINEEITSNQINTVPTFRVVQGRQNIYADIAQLIENSVDTIYIATTLDDVSRMYHSTIPEKISICEKNGGKVRLLVDMNDPKLAPFVKRFNATETKIGKMPSKGRIVVQKDKKMIMSDHAASSQYSGSDSDFSLCTNSNEMVDNIFTLCSFLWDSSKPLKTIDVKNFIKKTSEDQSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSF2</accession>
    <name>A0A2S2KSF2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12720</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866885.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSF2"/>
    <dbReference type="GeneID" id="76209229"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="67" mass="8115" checksum="FBFEF7C4DB4FF483" modified="2018-07-18" version="1">MMSNLDESIGKILLELQKDDPDTFKKLMKRAQEENPELFEEDINNENKEIEEYNYKVDEYNKKIDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSF7</accession>
    <name>A0A2S2KSF7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">P-II family nitrogen regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11790</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSF7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30115">
        <property type="entry name" value="NITROGEN REGULATORY PROTEIN P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30115:SF11">
        <property type="entry name" value="NITROGEN REGULATORY PROTEIN P-II HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00340">
        <property type="entry name" value="PIIGLNB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00938">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="11873" checksum="3C9EDA96BDF9BC40" modified="2018-07-18" version="1">MKMKRIEATIQATKIGAVTEAINDLVGGFTILEGKGRGSGIRQQVRSGRGTGSITKDYNSVATVSTIVDDTDVEKVSNAITDAAFTGKGGDGIIVVSNVENVTNIATKKSGSEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSH2</accession>
    <name>A0A2S2KSH2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Malate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_13080</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the malic enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSH2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004470">
        <property type="term" value="F:malic enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="entry name" value="Malic enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051674">
        <property type="entry name" value="Malate_Decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015884">
        <property type="entry name" value="Malic_enzyme_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43237">
        <property type="entry name" value="NADP-DEPENDENT MALIC ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43237:SF4">
        <property type="entry name" value="NADP-DEPENDENT MALIC ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00331">
        <property type="entry name" value="MALIC_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="7">
        <location>
            <begin position="14"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme NAD-binding" evidence="6">
        <location>
            <begin position="160"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="383" mass="41806" checksum="A1BE231ACFB295C4" modified="2018-07-18" version="1">MLSKNSLLLHEKLKGKILVDSKISNFKSKDLKLIYTPGVASVCDEIIKNPNSKFTLTSKGNNIAIVTDGTRLLGLGNVGADAALPVMEGKSVIYREYGKISAFPICLNTTDKKKIIDTILAIEPVFGAINLEDIESPKVLEIYKELEKRLAIPVFHDDGQGTAIVTLAGLINAVKVVKKNFSEIKIVLVGAGSAGYYIGKLLHFAGCKNIILLDSGGVVSQNRKSNMTRYKKEIAKLTNPKEKGNLSDVIKNADVFIGVSGIKDLLKPEMIKTMNKNPIIFALTNPNPEITPSIAKNAGAKIIATGSFTYKNKINNAIVFPYLMRAILDLKIQKITMNILYRTALAIAKTIPDKKLSSINIIPTIENKALQKNIMNSLKNLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSI9</accession>
    <name>A0A2S2KSI9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ATP--cob(I)alamin adenosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_12160</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877063.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSI9"/>
    <dbReference type="GeneID" id="76209288"/>
    <dbReference type="OrthoDB" id="4665at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008817">
        <property type="term" value="F:corrinoid adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1200.10:FF:000003">
        <property type="entry name" value="ATP:cob(I)alamin adenosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1200.10">
        <property type="entry name" value="Cobalamin adenosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016030">
        <property type="entry name" value="CblAdoTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036451">
        <property type="entry name" value="CblAdoTrfase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029499">
        <property type="entry name" value="PduO-typ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00636">
        <property type="entry name" value="PduO_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12213">
        <property type="entry name" value="CORRINOID ADENOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12213:SF0">
        <property type="entry name" value="CORRINOID ADENOSYLTRANSFERASE MMAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01923">
        <property type="entry name" value="Cob_adeno_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89028">
        <property type="entry name" value="Cobalamin adenosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cobalamin adenosyltransferase-like" evidence="6">
        <location>
            <begin position="3"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="178" mass="19958" checksum="04D1F7499BC61372" modified="2018-07-18" version="1">MKIYTKTGDDGNTGLQGNFRISKSHPRIIAYGAVDEVNAALGIVLTNILDDDIVKLLTKIQNDLFLVGADLSNPNLNDVKNRVTLEMIQNLEHSIDKFESELPPLTNFILPGGSIAGAQLHYVRTIVRRAETQIVQLSEKDEINSNCIIYLNRLSDLLFVMGRLINKRKGKDDILWKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSN3</accession>
    <name>A0A2S2KSN3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_14500</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877253.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSN3"/>
    <dbReference type="GeneID" id="76209045"/>
    <dbReference type="OrthoDB" id="9034at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="78" mass="8946" checksum="6EADDE83782BF1D7" modified="2018-07-18" version="1">MHDTEPDTFVYQTWPEKFSGMLKAIGIDSEPKEIGTDDIEKGDYYSRYFAHTARMITNRGCLDVKNSNIDTIQIIQKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSP9</accession>
    <name>A0A2S2KSP9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877176.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSP9"/>
    <dbReference type="GeneID" id="76209138"/>
    <dbReference type="OrthoDB" id="11747at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="135" mass="15273" checksum="267FBF949554E656" modified="2018-07-18" version="1">MARKLGPPIFLGIFTILAIVFLVGGGTDDKDVLRQTFQIDAVYYDTGHVEISYHDKSDKTNSVVMEILGMDESFQKTFSDSEFIEIVPFPNIPKYGWSVHPIVLEIDHEELGHVQLKTEIHLIDETAPPVIYSRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSS0</accession>
    <name>A0A2S2KSS0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ATPase AAA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_14900</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877287.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSS0"/>
    <dbReference type="GeneID" id="76209005"/>
    <dbReference type="OrthoDB" id="77269at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19509">
        <property type="entry name" value="RecA-like_VPS4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001054">
        <property type="entry name" value="ATPase, AAA family, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF83">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04212">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00745">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIT" evidence="8">
        <location>
            <begin position="5"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="148"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="85"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="397" mass="44936" checksum="C7B74E817703002F" modified="2018-07-18" version="1">MSLAPQELENTASKYASEAIKFDSQGARGMAITHYQHAIDSLVKLLQLYPSSKLNQIYKDRCNSYHNRINALQQAHGVEPAVDPKASEEEQKKSVQRQEDENDFEDLLMKEKPNVTWDQVIGLDDAKSALRESIVYPTKRPDLFPLGWPKGMLLYGPPGTGKTMLAAATANEMDGYFINVDASSMMSKWLGEAEKNVSKLFAMARQYAEKEGKPVILFVDEVDSLLGSRNSEVGGEVRTKNQFLTEMDGVNGKGKDLMLYVIGATNKPWSLDWPFLRRFQKRIYVSLPTQEARENLFAQYTAPLSKNYKVNNSELAKLFDGYSASDIKDVCQAAQIKTVHEIFNAPDYHEPVEGEEPVQPRELTTSDFKDIMARRKPSVSLEMIRAYHKWSEEFQAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSU2</accession>
    <name>A0A2S2KSU2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">MBL fold metallo-hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_15330</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877368.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSU2"/>
    <dbReference type="GeneID" id="76208456"/>
    <dbReference type="OrthoDB" id="40950at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16295">
        <property type="entry name" value="TTHA0252-CPSF-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050698">
        <property type="entry name" value="MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203:SF52">
        <property type="entry name" value="MRNA 3-END PROCESSING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16661">
        <property type="entry name" value="Lactamase_B_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="2">
        <location>
            <begin position="13"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-Casp" evidence="3">
        <location>
            <begin position="229"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="421" mass="46993" checksum="11111988D9E92C32" modified="2018-07-18" version="1">MNVKVLGAANEVGRSGFLVNCNGTNLLLDYGVLFGRRGTPPQYPLHVKPKDLDAIIISHAHLDHSGNVPSLFVSGNTDVYATPPTFDLSKLLIEDMLKIEKNSHPFDLPEVNNMMKNAKEIGFKQKITKGNATFELRESGHVIGGSTVLVESEKRRLFYTGDIKTHGSRMLREMDLDIGEIDMLITESTYAKTEQKPRKESESELIEFANEVMDRKGILFIPSFSVERSQEIACVLRSSNFKHKIIMDGMALKVNEIMFRYPEYLRDPKIFSDAIKSATAITEHADRKRAMEEPCVVISPAGMLVGGNAVYYLQQLSFDSKNGIALVSYQGEGTPGRKLLETGKVSTRGKDLNVEAEVRQFEFSGHADRKELFDMIKKIKGNPKVLTVHGDSESCDIFAQEIHEKFGLEAFSPAVNEEITV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSV2</accession>
    <name>A0A2S2KSV2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endoribonuclease L-PSP/chorismate mutase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_14950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877291.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSV2"/>
    <dbReference type="GeneID" id="76209000"/>
    <dbReference type="OrthoDB" id="371655at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02199">
        <property type="entry name" value="YjgF_YER057c_UK114_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013813">
        <property type="entry name" value="Endoribo_LPSP/chorism_mut-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43760:SF1">
        <property type="entry name" value="ENDORIBONUCLEASE L-PSP_CHORISMATE MUTASE-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43760">
        <property type="entry name" value="ENDORIBONUCLEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14588">
        <property type="entry name" value="YjgF_endoribonc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endoribonuclease L-PSP/chorismate mutase-like" evidence="1">
        <location>
            <begin position="5"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="151" mass="16529" checksum="2859339A1AF35A78" modified="2018-07-18" version="1">MIEEKLDELGIKLPNPPIPAGSYVPAIRTGNLLFISGQIPMEDGKVIFTGKVSDENLETAQKSARMCAINILTQIKRELGDLNKVSKIVRLSGFVNSVPEFSQQPKVINPASDLFFEIFGEKGKHSRIAVGVSCLPLNSMTEIDAVVEFLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSX1</accession>
    <name>A0A2S2KSX1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase M10 metallopeptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_15150</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877309.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSX1"/>
    <dbReference type="GeneID" id="76208981"/>
    <dbReference type="OrthoDB" id="9634at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M10 metallopeptidase" evidence="6">
        <location>
            <begin position="218"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="282" mass="31270" checksum="05B7100DCB744001" modified="2018-07-18" version="1">MLGISYVLKNKKLIHFTKKNLTHKTKVSKIKHQKSILVLQIGILIFFGGYFTGLPSSSGENEELLNYVGFFNSEHTVENLRGDSISLSKYWRLSQGSSLSVNILNPVSISNESIETIKESILSKEKINLEDSLLHKGPQNSFSNYYVGWAGALENTPETKIPIPKNFEVLNSQKSNGDIVVILSNIKDRSGNTGYTKTILEGEEIVKAFITIYDVDSLSNTQLGTIMRHEFGHALGLGHSTAPEDLMAPTIDMTYPYISDCNIIAVADLYNENSDGTTICEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSY3</accession>
    <name>A0A2S2KSY3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSY3"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="51" mass="5961" checksum="3174E40BFF8A66E5" modified="2018-07-18" version="1">MLKYAGIYQRESRQSMFKKIIEKLKFGSSKAKVTEDVNAFKESEKKQDSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSZ2</accession>
    <name>A0A2S2KSZ2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Roadblock/LC7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_14680</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877267.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSZ2"/>
    <dbReference type="GeneID" id="76209027"/>
    <dbReference type="OrthoDB" id="8019at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14679" checksum="084E39AD1A97116D" modified="2018-07-18" version="1">MAKKDFETICNTIATISPYIRFVGVIGESGELLAYKRRPDLIPLLDEKNTQYQFSHIAMKTDLEGFFDKNLGEIEFSWEERKKVQTISFAIKKIRVWMSIDKKVIRSEVLRIIDSCLPIVKKYSNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT19</accession>
    <name>A0A2S2KT19_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Transcription initiation factor IIB</fullName>
            <shortName evidence="7">TFIIB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">tfb_8</name>
        <name evidence="7" type="synonym">tfb</name>
        <name evidence="11" type="ORF">NZNM25_13960</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the TFIIB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877209.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT19"/>
    <dbReference type="GeneID" id="76209103"/>
    <dbReference type="OrthoDB" id="7429at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20549">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20550">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000023">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00383">
        <property type="entry name" value="TF2B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023484">
        <property type="entry name" value="TFIIB_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="10">
        <location>
            <begin position="8"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="repeat" description="1" evidence="7">
        <location>
            <begin position="124"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="repeat" description="2" evidence="7">
        <location>
            <begin position="218"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="112"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="303" mass="33077" checksum="C691AA5815425212" modified="2018-07-18" version="1">MLKNTMISGPKCPSCGNKKMVTDQDTGELFCSKCGIVVTDKIADTGAEWRSFSNDETNRTRVGAGTSLTMHDMGLSTVIGAANKDSTGKPLSASVKSSIERLRTWDSRTQAHSSADRNLRQALNEMDKLKDKLALTDAVVEKAAYIYRKAMERKLVRGRSIQGLVAACLYASCRNTETPRTLDDIAKGINIRRKDVARCYRLIFRELELKMPVVDPVKGVSRIASIAELSEKSKRKAIEILNQAKKIGMVAGKDPMGIAAAALYLACISTGEVKSQKDISVASGVTEVTIRNRCAGLRKMLND</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT38</accession>
    <name>A0A2S2KT38_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phasin family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_15900</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877427.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT38"/>
    <dbReference type="GeneID" id="76208397"/>
    <dbReference type="OrthoDB" id="2022at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="137" mass="15535" checksum="0DBBE9F76C69B992" modified="2018-07-18" version="1">MSTQTQSNNVYSMYKQNVQKYFENISKIIPQYFQAMTQLQEECMKTCEKTINASVSVQQEFAKKTGIISDVPDATKTAIIDTNKQIVQASTVNNQLVKTTIDATVQNIKTFNDNINAFAELNKNTIQSWINPITQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTC7</accession>
    <name>A0A2S2KTC7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribonuclease P protein component 4</fullName>
            <shortName evidence="8">RNase P component 4</shortName>
            <ecNumber evidence="8">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Rpp21</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rnp4</name>
        <name evidence="9" type="ORF">NZNM25_16220</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the eukaryotic/archaeal RNase P protein component 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="8"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTC7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00757">
        <property type="entry name" value="RNase_P_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016432">
        <property type="entry name" value="RNP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007175">
        <property type="entry name" value="Rpr2/Snm1/Rpp21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04032">
        <property type="entry name" value="Rpr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004878">
        <property type="entry name" value="RNase_P_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="7 8" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="109" mass="12465" checksum="AF1130F7215BFB3E" modified="2018-07-18" version="1">MKSAVRKIAIERMQILIEKAILNAKINPDLSQRQAFLARRISTRHKIRMPYALRMVFCKKCKSFIAPGLNSRIRIGRTSVKSIRISCNLCGHTYRKVIPPSATIKKNSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTC8</accession>
    <name>A0A2S2KTC8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_16950</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTC8"/>
    <dbReference type="OrthoDB" id="372227at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:CCA tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059101">
        <property type="entry name" value="NFACT-R_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020536">
        <property type="entry name" value="ThiI_AANH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11933:SF6">
        <property type="entry name" value="THIL AANH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11933">
        <property type="entry name" value="TRNA 5-METHYLAMINOMETHYL-2-THIOURIDYLATE -METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18297">
        <property type="entry name" value="NFACT-R_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02568">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thil AANH" evidence="3">
        <location>
            <begin position="23"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="NFACT protein RNA binding" evidence="4">
        <location>
            <begin position="250"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF18297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="352" mass="39308" checksum="AB8DCC81E151EFED" modified="2018-07-18" version="1">MYDVKFVYGKLSLIAVTEEKEKKKVVALLSGGLDSQLAVRMMQEQGFDVSAVAIKTPFCDFDCGRGCGFEIRERADDLNVNLKTVYLGDEYIEMLKHPKHGIGAGFNPCVDCRTMMFDAAKKYMEEIGAEFIISGEVLGQRPMSQHAPALRIIEKESGLIGKIVRPLSAGLLPETDPEKEGLIKRENLGMIKGRTRRNQLEMAKKYGIENPPNAGGGCLLTEPQFGIKAKDLFEHVETPTINDIDLLKIGRHFRLDEETKFVVGRNKDENEMIKAIALPGDILLEAKDHLGPISILRGKNAKDHVEFAASVTLRYSDAPKDQDCIVTVKNENLTEEINSQCAEEQSYIKFRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTP5</accession>
    <name>A0A2S2KTP5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Universal stress protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_17420</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877550.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTP5"/>
    <dbReference type="GeneID" id="76208243"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF25">
        <property type="entry name" value="USPA DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="4"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="140" mass="14903" checksum="241BFF71F79AEF69" modified="2018-07-18" version="1">MAKFNKILVPLDGSANSIRGLDRAIDIAKGGNAEITGFYVFHLPLTAGIKYTQKMKDDAQKKAVKAIGPAMNRAQKAGATFKYKTGGGHTGSEIVKFAQNGKFDMIVIGARGMGGAKEAFLGSTSNYVMHKTKIPVLVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTQ0</accession>
    <name>A0A2S2KTQ0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_17970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTQ0"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="301" mass="33269" checksum="99E60B1C1E285CB1" modified="2018-07-18" version="1">MVPDIDAETITRNLEGGMDIQITYPEEIVVGREGVISILVKNNGWENKENISFGISLSAIPGLITEPSDKITLEKLAQGGSYGENISLQIPSDASLGVHYLNLKYSHVLVANNETPKPPFFHDIAIPLTIKKDPSVTIHTKMPESIFSNAEFPIEIEVLSKDIDITNVGIKIIPPKDIEFRGETLHTFSNIQKNMPIAVTSQIITPVKEVNTEYKLPFQIIVTYTDDIGLDKEDSQTVSVVLRPRTFMELTTDGGIWIGSFFIAPYVSLGTIIGIPAGAIISLLIRKKQNSSKKRTKKKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTT4</accession>
    <name>A0A2S2KTT4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_18440</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866934.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTT4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd05819">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001258">
        <property type="entry name" value="NHL_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050952">
        <property type="entry name" value="TRIM-NHL_E3_ligases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24104">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NHLRC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24104:SF25">
        <property type="entry name" value="PROTEIN LIN-41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17170">
        <property type="entry name" value="DUF5128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01436">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51125">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="368"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="468"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="579"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="545"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="565"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH35053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="867" mass="94671" checksum="80ACF7A43E5219D7" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTV1</accession>
    <name>A0A2S2KTV1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PhoU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_16860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTV1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930:SF5">
        <property type="entry name" value="PHOSPHATE UPTAKE REGULATOR, PHOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PHOSPHATE-SPECIFIC TRANSPORT SYSTEM ACCESSORY PROTEIN PHOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109755">
        <property type="entry name" value="PhoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PhoU" evidence="1">
        <location>
            <begin position="122"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="310" mass="35220" checksum="056182B7ED2D2987" modified="2018-07-18" version="1">MSLPKEWIDANNLDKSSQVEIETGHDSISISLNKESRTTKELVISYPLPKEENIVADITGAYLLGYDVFKINSKSSIPGEDREKIRNSMRRLVGMEIIEEDASHINMQFLLDATTLDPEKILKRMSSIALGMYDDASKGLISDDKSNLETLSKRDVEVNRQYFLLVRLIRSALVDKKLANAFNLENIDVLDYRVAANLLENAGDSIVELSNFIYNSTLSQEDSKKIFDMVKDFSILAEKSIDAFTKPDRLLAIEAISLHKKYEQNLSKLRIALGNKKQIPIEFLDLVYMYEKIAQSWADVADLVKPIYNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTY2</accession>
    <name>A0A2S2KTY2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_18800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877703.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTY2"/>
    <dbReference type="GeneID" id="76210107"/>
    <dbReference type="OrthoDB" id="1506at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058349">
        <property type="entry name" value="DUF8036"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26119">
        <property type="entry name" value="DUF8036"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="94" mass="10209" checksum="EBFFF585FBD52941" modified="2018-07-18" version="1">METTSLILSIVSIANMGILGILIAIFGNMYSKTRAQLPLGMIVVAGMLFLHNVIGSLAYFSMEEIFSHDIFPYMLGVGIAELAGLLIFLKITLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU44</accession>
    <name>A0A2S2KU44_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Thermosome subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_17690</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877575.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU44"/>
    <dbReference type="GeneID" id="76208216"/>
    <dbReference type="OrthoDB" id="9362at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03343">
        <property type="entry name" value="cpn60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012714">
        <property type="entry name" value="Thermosome_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02339">
        <property type="entry name" value="thermosome_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="529"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="531"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="570" mass="60911" checksum="F888B03F2CBB1122" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU55</accession>
    <name>A0A2S2KU55_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_19910</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877797.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU55"/>
    <dbReference type="GeneID" id="76210222"/>
    <dbReference type="OrthoDB" id="11787at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="110" mass="12986" checksum="F4751E1A0773309D" modified="2018-07-18" version="1">MEGIISFGNKRNYEDFKKQIPSSVLPLFDSIREFCFSLGENVVEDIRMHRVVFCKSITFRWFADVEPQKEGVIIKIQKNRKEPIHIIQISKEQKISEFGNLIKEAFEKIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUG9</accession>
    <name>A0A2S2KUG9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">AmmeMemoRadiSam system radical SAM enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_18890</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877711.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUG9"/>
    <dbReference type="GeneID" id="76210116"/>
    <dbReference type="OrthoDB" id="5682at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027596">
        <property type="entry name" value="AmmeMemoSam_rS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034457">
        <property type="entry name" value="Organic_radical-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016431">
        <property type="entry name" value="Pyrv-formate_lyase-activ_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04337">
        <property type="entry name" value="AmmeMemoSam_rS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352:SF5">
        <property type="entry name" value="PYRUVATE FORMATE-LYASE 1-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352">
        <property type="entry name" value="PYRUVATE FORMATE-LYASE-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004869">
        <property type="entry name" value="PflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01101">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="72"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR004869-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH35098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="356" mass="40611" checksum="8D3B3EAF633F2134" modified="2018-07-18" version="1">MTTILGKEAELYEKLPNDKVKCTACARYCEIGKGQIGLCGIRGNENGKLHLYAYGKVISGHVDPIEKKPLIHYFPGSKVYSIATTGCNWLCRYCQNSDISQRRKVEGIDMTPQDVANTAVKYGAHGIAYTYNEPSIFIEFARDCGIAARKKGLFNVFVSNGYDTPESVSMMNEFLDGITVDFKGSAEKEFTRKFIGIPDPQPIFDTLLEIRDKTKIHIEITDLIVPKVGDDLTHAKKLSKFIYDEFGPEMPIHFLRFHPDYKMMEYPSTPVETLEKHYQIAKDQGLKYVYLGNVPGHKWEHTYCSECNKIVVNRYGFSIRGWHLDKNNCCQFCGNKIPIEGKLQKGYKEDRFQFVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUJ9</accession>
    <name>A0A2S2KUJ9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aminotransferase</fullName>
            <ecNumber evidence="5">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_19190</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="5"/>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877732.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUJ9"/>
    <dbReference type="GeneID" id="76210148"/>
    <dbReference type="OrthoDB" id="372018at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051926">
        <property type="entry name" value="Ala_Aminotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006230">
        <property type="entry name" value="PRK08363.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43488">
        <property type="entry name" value="GLUTAMATE-PYRUVATE AMINOTRANSFERASE ALAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43488:SF2">
        <property type="entry name" value="GLUTAMATE-PYRUVATE AMINOTRANSFERASE ALAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="6">
        <location>
            <begin position="34"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH35128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="391" mass="43390" checksum="EDA311799A92BE6B" modified="2018-07-18" version="1">MKVSKKVVGVEYAIRDIVVAARKVQQKGMNVDYLNIGDPVQFGFQPPDNVKQALIDAINRGENYYSTSEGLLELREEIAKKENTKGLSISSDEILITNGVSEGLDMVISSIVEEGDEVLLPGPYYPPYASYVRLHGGVPIEFAVDLENSTPDIEDIKSKITSKTVAICLISPNNPTGVVFNEKSLKELVNIANQNNLYIICDEIYDQIIFDDEFVGIGKVAGDSPVIVLNGFSKVHLMSGWRIGYIAFNQSSKLDNLREHLPKLARVRIATSLPVQHAALESLRGPQNYISEFVSEIKKRRDLVVKRLNEMPGLSCPNPKGAFYAFPKIEDNRFGTDKEFVTKLLETKGVLTVHGSGFGEQYGSGHFRLVYLPSLEILDSAMNKIQDFVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUL9</accession>
    <name>A0A2S2KUL9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_19660</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877776.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUL9"/>
    <dbReference type="GeneID" id="76210196"/>
    <dbReference type="OrthoDB" id="4539at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13692">
        <property type="entry name" value="Glyco_trans_1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="326" mass="37769" checksum="D86A4E0405462742" modified="2018-07-18" version="1">MTKVSMITEHKIDGINASGNRAHWEIKALKRKGFLDIELIDDFDETKVSKLSNNLVHAQQHSGRFLENIPYIVDAHGLEYVYSAHMSYGYPFHSWRRWAFKAKSYHYKKLETKIFQNSKHIICAGENIYEKVKNIQNSTIVRNAVFPESYIPTNCKSLKIALVGPFLPGKLNYFGFDMIKFIVKKFQNIDFVFIGPADKYFRNGLKFKNTTFTGKVNNYIETLRTCSVLLAPYPDYAYYLGSKTKFVEAAACQMPIITTPVGNIDFQNDYVCIGKTKEDLVNQIYYLEDESVRLDLGKKLRNEIFKKYNAEIEIQKVIKLYDELTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNR0</accession>
    <name>A0A2S2KNR0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00810</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866792.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNR0"/>
    <dbReference type="GeneID" id="76209410"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="81" mass="9531" checksum="28865C17B331E5A0" modified="2018-07-18" version="1">MRLAFVTLEEQITRIMKNFESASSEEILEILNKIMPEFKSELTSEYLQGKIQKITQIDDESEKIKQCKALTPYLDWYLQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNR8</accession>
    <name>A0A2S2KNR8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Dehypoxanthine futalosine cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_00940</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875970.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNR8"/>
    <dbReference type="GeneID" id="76209422"/>
    <dbReference type="OrthoDB" id="8186at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044689">
        <property type="term" value="F:7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045567">
        <property type="entry name" value="CofH/MnqC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034405">
        <property type="entry name" value="F420"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020050">
        <property type="entry name" value="FO_synthase_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00423">
        <property type="entry name" value="CofH family radical SAM protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076">
        <property type="entry name" value="FO SYNTHASE (COFH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076:SF14">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19288">
        <property type="entry name" value="CofH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004762">
        <property type="entry name" value="CHP00423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00343">
        <property type="entry name" value="aminofutalosine_synthase_(mqnE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00342">
        <property type="entry name" value="cyclic_dehypoxanthine_futalosi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01064">
        <property type="entry name" value="F420__menaquinone_cofactor_bio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01389">
        <property type="entry name" value="menaquinone_synthsis_involved"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="8">
        <location>
            <begin position="54"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR004762-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR004762-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="365" mass="40596" checksum="C40977ED92975E3E" modified="2018-07-18" version="1">MLGQLVGDSQALDRAIAGEELSYKDGLELMNYDNQHILAAVADNSRKKLVGDTVTFAASYYMNYTNVCAASCQMCAFYRKDGADDAYTLTSQEIEQRVGIAEQMGATEVHIVGGFHPTLPLEYYEDMMKVIKKNHPQLNIKALTAAEIFFLSKLTKNSTKEILSRLKDAGLDSMPGGGAELFHPDIRDKIVRGKCTGQQWLDVIEEAHNMGIQSNVTMLYGHIEKPEHIIDHLIKIRELQKKTKGFITLIPLKFSLDNTELEQQHLVNNECSSLYDLKIIALSRLMLANVLNNISVYWVAYGKKLAQVALSNGGSDLVGTAFSEEIYRAAGKPTASSIEELATMVKEIGRSPAQRNTHFGILREF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNX7</accession>
    <name>A0A2S2KNX7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_00680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875945.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNX7"/>
    <dbReference type="GeneID" id="76209397"/>
    <dbReference type="OrthoDB" id="11629at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="274" mass="29442" checksum="07DBE9D1B0B25E8B" modified="2018-07-18" version="1">MNERLESVSKGKKLIILGFITIIALFILYGRYQDPELLTPSAIDSIQRIAYGFYITLVAAFGAIAFGLYRYHKGKVMANQKNLSTIIALTTWNSKSRKIFVVTFIGYGMFFSLASGTLVYQPEVNFAIHYGATIPSGFIAPCCDGPGYMPKIIVYLTEHVGLQIIPINLVLQVIVSYLVGLNAAIAVSAYGISKKGRGASTIGAATGLFIACPTCAGTFLSIFIGTASGIALSIALTQMQTLFIAISIPVLIITPFVMAKKLQNSDGSCKIDPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNZ7</accession>
    <name>A0A2S2KNZ7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_01910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876059.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNZ7"/>
    <dbReference type="GeneID" id="76209522"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="155" mass="17741" checksum="26B64B4CAFEEBFB9" modified="2018-07-18" version="1">MHIEKLGKVGIGVGIAVGIMMLFMLGMSIPDQVGRFIQDYDVSRFEENPSELQTVNAEFLMEKFSQTEDYAIFVQRNPEYVEDLELYRDGGHLRLTAFNTTTNNVLELRLSWDNDDFIKMDKDAYCRIGNSLDRAEITARESAVSHFLKTTDCVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP19</accession>
    <name>A0A2S2KP19_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_02240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876090.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP19"/>
    <dbReference type="GeneID" id="76209558"/>
    <dbReference type="OrthoDB" id="8405at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="71" mass="8756" checksum="5ED9E933E4409A9E" modified="2018-07-18" version="1">MKNAECKKCLNKFQQKDIYTIQQFQYRKIPTYKWSLNFFKKLEINEWDSFCEKCITEYAEKSKEGWDKLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP24</accession>
    <name>A0A2S2KP24_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Adenosylmethionine-8-amino-7-oxononanoate aminotransferase</fullName>
            <ecNumber evidence="7">2.6.1.62</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">7,8-diamino-pelargonic acid aminotransferase</fullName>
            <shortName evidence="7">DAPA AT</shortName>
            <shortName evidence="7">DAPA aminotransferase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">7,8-diaminononanoate synthase</fullName>
            <shortName evidence="7">DANS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Diaminopelargonic acid synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">bioA</name>
        <name evidence="9" type="ORF">NZNM25_00090</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only aminotransferase known to utilize SAM as an amino donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(8S)-8-amino-7-oxononanoate + S-adenosyl-L-methionine = S-adenosyl-4-methylsulfanyl-2-oxobutanoate + (7R,8S)-7,8-diammoniononanoate</text>
            <dbReference type="Rhea" id="RHEA:16861"/>
            <dbReference type="ChEBI" id="CHEBI:16490"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:149468"/>
            <dbReference type="ChEBI" id="CHEBI:149469"/>
            <dbReference type="EC" id="2.6.1.62"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; biotin biosynthesis; 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.62" evidence="7"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_264953971.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP24"/>
    <dbReference type="GeneID" id="76209335"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00160"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004015">
        <property type="term" value="F:adenosylmethionine-8-amino-7-oxononanoate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00834">
        <property type="entry name" value="BioA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005815">
        <property type="entry name" value="BioA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00508">
        <property type="entry name" value="bioA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42684">
        <property type="entry name" value="ADENOSYLMETHIONINE-8-AMINO-7-OXONONANOATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42684:SF3">
        <property type="entry name" value="ADENOSYLMETHIONINE-8-AMINO-7-OXONONANOATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5 7" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7 9" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="92"/>
            <end position="93"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="295"/>
            <end position="296"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Participates in the substrate recognition with KAPA and in a stacking interaction with the adenine ring of SAM" evidence="7">
        <location>
            <position position="7"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="427" mass="48655" checksum="340EAF26A5C8DAB5" modified="2018-07-18" version="1">MKEWSLFDEITSAKGVWLRDSKGNKTLDAVASMWCNVWGHSNQELINAITNQSKKLQHSSMFNLTNEPAEKLAEYLVKISPKMHKVFYSDNGSSAMEIAIKMALQYWKNIGEKNKTKIATLENGYHGDTFGAMSVGYVPEFFGKFKKQLFTTLQFPVPNKYRVPKGFTFLDYQNHCLEKIENEFAKRNNIAAFIMESGAQVAGGVIIYPKGFQNKINKLCKEYDVIFVLDEIATGFGRLGSMFQFQEQKNTPDIVAYGKMLTGGYLTMAATLTSKKVYDSFLGEFNDWKHLFHGHTYTGNPIAAAVALENLKMYKKYKLIEKIQSTSKIFEKYYQKILEIDIVGDIRHKGMLMGIELVTDKKKKTPIHPKKSINKIFFEEGKRHGIYLRTLGNIVMIVPPLAITKKELELLLEKTILTIKAARAQVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP37</accession>
    <name>A0A2S2KP37_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_02310</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876095.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP37"/>
    <dbReference type="GeneID" id="76209565"/>
    <dbReference type="OrthoDB" id="31361at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="94" mass="10778" checksum="E116B74177E134BC" modified="2018-07-18" version="1">MAELEHKYEVDISEKEGRQKLPQDVKDELKASGMMDEKGKLKLKGVSPKMLKKMKQEFVDCPVLKEEIQFIQCFVCPNFQSRVMGKVLCKGDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP40</accession>
    <name>A0A2S2KP40_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_02440</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876106.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP40"/>
    <dbReference type="GeneID" id="76209578"/>
    <dbReference type="OrthoDB" id="9320at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="82" mass="9693" checksum="3A5D877A49B67634" modified="2018-07-18" version="1">MDYPVSADENGINLKPEKMEKEKLYHCIFKNKAMLVFKDSQDVLNCYEIEHEDLVEKIRKASNEDQLEKILEDYLDGQNLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP41</accession>
    <name>A0A2S2KP41_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_01450</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876014.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP41"/>
    <dbReference type="GeneID" id="76209475"/>
    <dbReference type="OrthoDB" id="3363at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="508"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="570" mass="66407" checksum="8B34DDD7948DAEB8" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP43</accession>
    <name>A0A2S2KP43_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rps17</name>
        <name evidence="7" type="ORF">NZNM25_00290</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875911.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP43"/>
    <dbReference type="GeneID" id="76209355"/>
    <dbReference type="OrthoDB" id="10698at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00364">
        <property type="entry name" value="Ribosomal_uS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01345_A">
        <property type="entry name" value="Ribosomal_uS17_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028333">
        <property type="entry name" value="Ribosomal_uS17_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019978">
        <property type="entry name" value="Ribosomal_uS17_archaeal"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006345">
        <property type="entry name" value="PRK08572.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03630">
        <property type="entry name" value="uS17_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744:SF9">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="107" mass="11885" checksum="A4BE80C2219D53EB" modified="2018-07-18" version="1">MTRNIGLKVKEPKGECADKHCPFHGNLSIRGKLFDGKVTGSKARQTITLQKDAPIYFSKFKRYARGTSTIHAHVPECINVETGNHVLTAECRPISKSVSYVVVEVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP49</accession>
    <name>A0A2S2KP49_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Lysine transporter LysE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_02410</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876103.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP49"/>
    <dbReference type="GeneID" id="76209575"/>
    <dbReference type="OrthoDB" id="121309at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001123">
        <property type="entry name" value="LeuE-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38825">
        <property type="entry name" value="LYSINE EXPORTER PROTEIN (LYSE/YGGA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38825:SF1">
        <property type="entry name" value="TRANSPORTER, LYSE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01810">
        <property type="entry name" value="LysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="206" mass="22883" checksum="A80789A872737A45" modified="2018-07-18" version="1">MEQFFEFALSVIVISASGVMAPGPLFAANVSYGLREGARSGIKMAVGHTIVEFPLIVLLGVGVFSFEIFPEFRTIISILGAITLFVFAIIQIKTVFTRKEIAKIKTKQGPLITGILLSALNPFFIIWWFTIGFKLISDAMLIWAFAGILILFALHIWMDFAWLGAISFFTSKSSKILSNRNYKMLMISLSAALIYFGISFLSDIVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP69</accession>
    <name>A0A2S2KP69_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_02740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876131.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP69"/>
    <dbReference type="GeneID" id="76209611"/>
    <dbReference type="OrthoDB" id="9652at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00156">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051797">
        <property type="entry name" value="TrmB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="1">
        <location>
            <begin position="292"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="404" mass="46053" checksum="3DC818B5E95B848C" modified="2018-07-18" version="1">MQVQDISLEEELNQKEERDEISILRDFGLEEDEAQTYVGLAQLGSVKASEISAFTKIDRVRTYKILENLKNLGFATSTLSSPIKFSANDPEMILKDIILKQKQKVEHLEKNSSQFLKILSRLKLHEPQIGLPKLTIVSNRNNIYDQMTKIIEETKDRLYIVVTLSDIIRMYYTSIPEAIKKATKQNTKIKLMTGPELSTKLEYIQRLGINKFRIVTLPSPGRLLCSKAQVLMSGNTSSQVNKNTNEESVMVTNSNDIIKNMQSLCEFLWESGEDVSIEDNSKKEKKHEKESTILVVDDDPDAVNIFADYLEIKGVSTVETCTDGKKAIEAFKKIRPDAVFLDIMMPDFDGFYVLEQIRKIDPKAKIIMVTADKSSATNKKLHKVKPTDVIYKPYDIEQITRCLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPK6</accession>
    <name>A0A2S2KPK6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_03830</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876228.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPK6"/>
    <dbReference type="GeneID" id="76209725"/>
    <dbReference type="OrthoDB" id="18492at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03217">
        <property type="entry name" value="ABC_FeS_Assembly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010230">
        <property type="entry name" value="FeS-cluster_ATPase_SufC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01978">
        <property type="entry name" value="sufC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43204">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43204:SF1">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="4"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="256" mass="28182" checksum="6084EF19657675A3" modified="2018-07-18" version="1">MAVLEIKDLHVSREGKEILKGVNLKTGPGEVHAIMGPNGSGKSTLAYTLLAHPKYEVTKGDILLDGESILELSADERAKKGLFLGFQYPTEVSGVGFSHFLRTAYNSLSKALEGDDREVFITVREFQKYLKENLEKVGLKEEFLSRYLNEGFSGGEKKRAEVLQMAVLKPKISILDEPDSGLDIDAVQAVAQAISKVSGKDATVIVITHYARILKFLDKLDFVHVFARGQVLKTGDATLADKLEAEGYDWVLEQPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPQ7</accession>
    <name>A0A2S2KPQ7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPQ7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="91" mass="10473" checksum="F635079BF527CF08" modified="2018-07-18" version="1">MCGKNKRMWNKKTKMKYQCTKCSFQWEGTSYTFDNVRVHEKTHLPKNENISVRCKVCNTLKITKGLINSNGGWECKMCGNLINAQGHVITS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPQ8</accession>
    <name>A0A2S2KPQ8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_02560</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866802.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPQ8"/>
    <dbReference type="GeneID" id="76209590"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="985" mass="112372" checksum="919F3A68F04A40BE" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPS4</accession>
    <name>A0A2S2KPS4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03220</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_263970049.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPS4"/>
    <dbReference type="GeneID" id="76209660"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="43" mass="4985" checksum="B054D0C0005C116C" modified="2018-07-18" version="1">MMYNSRLFVCVKKLTKTRGLGEINVKNVPPEALICIVFNPFWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPS7</accession>
    <name>A0A2S2KPS7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL43</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl37ae</name>
        <name evidence="5" type="ORF">NZNM25_04630</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eukaryotic ribosomal protein eL43 family. Putative zinc-binding subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876333.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPS7"/>
    <dbReference type="GeneID" id="76209815"/>
    <dbReference type="OrthoDB" id="372011at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00327">
        <property type="entry name" value="Ribosomal_eL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050522">
        <property type="entry name" value="Ribosomal_protein_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="1 4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="zinc finger region" description="C4-type" evidence="4">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="70" mass="7884" checksum="BA5C9E2400A28578" modified="2018-07-18" version="1">MAKTKKSLKGLGARYGIKLRKQYTKIHLQLKEKRTCPECGSKTFGRDAVGIWSCKKCSYKVAGTAYDIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPT3</accession>
    <name>A0A2S2KPT3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 6">Shikimate dehydrogenase (NADP(+))</fullName>
            <shortName evidence="6">SDH</shortName>
            <ecNumber evidence="1 6">1.1.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 10" type="primary">aroE</name>
        <name evidence="10" type="ORF">NZNM25_03480</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>shikimate + NADP(+) = 3-dehydroshikimate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17737"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the shikimate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.25" evidence="1 6"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876196.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPT3"/>
    <dbReference type="GeneID" id="76209688"/>
    <dbReference type="OrthoDB" id="8744at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00087"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019632">
        <property type="term" value="P:shikimate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01065">
        <property type="entry name" value="NAD_bind_Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000086">
        <property type="entry name" value="Quinate/shikimate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00222">
        <property type="entry name" value="Shikimate_DH_AroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041121">
        <property type="entry name" value="SDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011342">
        <property type="entry name" value="Shikimate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022893">
        <property type="entry name" value="Shikimate_DH_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00507">
        <property type="entry name" value="aroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089:SF1">
        <property type="entry name" value="BIFUNCTIONAL 3-DEHYDROQUINATE DEHYDRATASE_SHIKIMATE DEHYDROGENASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089">
        <property type="entry name" value="SHIKIMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18317">
        <property type="entry name" value="SDH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="3 6" id="KW-0521">NADP</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Shikimate dehydrogenase substrate binding N-terminal" evidence="8">
        <location>
            <begin position="7"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Quinate/shikimate 5-dehydrogenase/glutamyl-tRNA reductase" evidence="7">
        <location>
            <begin position="113"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="SDH C-terminal" evidence="9">
        <location>
            <begin position="240"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="15"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="126"/>
            <end position="130"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH33557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="273" mass="30206" checksum="C0BE21D14EC8230E" modified="2018-07-18" version="1">MAKSFAVIGDPIDHSLSPNIHNAAFRELNLDCSYIAYRIPKGELEDGIEGLKKIKIDGFNVTIPHKVEMMKYLNKMDESCSLIGAVNTVTNNDGILKGYNTDMDGFLEPFKKKKLNIENTKVLLLGAGGAARAIVAAFAKEKAKSITIANRTLENARNLSEFAKKIGLDVNVIRIEDVKDSAKNYDIIVNATSVGLKNEPTIISLDNINEKTIVYDIVYMPINTDFIKKAKEKNAVIIFGYEMLLGQATRAFEIWHGMKAPYNAMKKALLGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPT5</accession>
    <name>A0A2S2KPT5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 7">16S rRNA aminocarboxypropyltransferase</fullName>
            <ecNumber evidence="7">2.5.1.157</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_02690</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Aminocarboxypropyltransferase that catalyzes the aminocarboxypropyl transfer on pseudouridine corresponding to position 914 in M.jannaschii 16S rRNA. It constitutes the last step in biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>an N(1)-methylpseudouridine in rRNA + S-adenosyl-L-methionine = N(1)-methyl-N(3)-[(3S)-3-amino-3-carboxypropyl]pseudouridine in rRNA + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63296"/>
            <dbReference type="Rhea" id="RHEA-COMP:11634"/>
            <dbReference type="Rhea" id="RHEA-COMP:16310"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
            <dbReference type="ChEBI" id="CHEBI:146234"/>
            <dbReference type="EC" id="2.5.1.157"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TDD superfamily. TSR3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.157" evidence="7"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876127.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPT5"/>
    <dbReference type="GeneID" id="76209604"/>
    <dbReference type="OrthoDB" id="7441at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106388">
        <property type="term" value="F:rRNA small subunit aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01116">
        <property type="entry name" value="TSR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022968">
        <property type="entry name" value="Tsr3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007177">
        <property type="entry name" value="Tsr3_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002621">
        <property type="entry name" value="PRK02287.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426:SF0">
        <property type="entry name" value="18S RRNA AMINOCARBOXYPROPYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN TSR3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="Fer4_RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04034">
        <property type="entry name" value="Ribo_biogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNase L inhibitor RLI-like possible metal-binding" evidence="9">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="16S/18S rRNA aminocarboxypropyltransferase Tsr3 C-terminal" evidence="8">
        <location>
            <begin position="38"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH33478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="163" mass="18583" checksum="5336C15931B7FE4A" modified="2018-07-18" version="1">MKLQVLMFYQDDPKKCTAAKMVKFGLAQNITKIGAKGLVLDPFSEKTLLPKDKFLINSIVGIDCSWNLAEHAFSKKFNGIKRKLPPLLAGNPVNYSKLNKLTTVEALSATLIILGFKEQGLELLDKFKWGHTFYELNQNLFEEYARLENEQQIQLILKDYGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPV5</accession>
    <name>A0A2S2KPV5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04930</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876359.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPV5"/>
    <dbReference type="GeneID" id="76209845"/>
    <dbReference type="OrthoDB" id="9879at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="175" mass="20122" checksum="A78D42D5516B54BD" modified="2018-07-18" version="1">MSSKKTSSISFRIDEEYEKVLRTIAKENKISLNTLANQIFGNYVEFEIYAKKFGTLRMSTDTLRRILNNIDEKTLIDIATRGGAQEAKEFILFKWKKITLDSVTDFIKIYFDLCGYGRCSLEKTASNIVFSVHHDLKEKGSLFLKQFLESLIKTTLEKDCDTTITEDTITISFQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPZ6</accession>
    <name>A0A2S2KPZ6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytidylate kinase</fullName>
            <shortName evidence="6">CK</shortName>
            <ecNumber evidence="6">2.7.4.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Cytidine monophosphate kinase</fullName>
            <shortName evidence="6">CMP kinase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cmk</name>
        <name evidence="7" type="ORF">NZNM25_05100</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>CMP + ATP = CDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:11600"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>dCMP + ATP = dCDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:25094"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57566"/>
            <dbReference type="ChEBI" id="CHEBI:58593"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the cytidylate kinase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.25" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876372.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPZ6"/>
    <dbReference type="GeneID" id="76209861"/>
    <dbReference type="OrthoDB" id="31096at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036430">
        <property type="term" value="F:CMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036431">
        <property type="term" value="F:dCMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006220">
        <property type="term" value="P:pyrimidine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00239">
        <property type="entry name" value="Cytidyl_kinase_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011892">
        <property type="entry name" value="Cyt_kin_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13189">
        <property type="entry name" value="Cytidylate_kin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01100">
        <property type="entry name" value="SHIKIMTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="186" mass="21276" checksum="5B3814649C8E66B3" modified="2018-07-18" version="1">MTKSIVISGPPAVGKTTVAKGLAEEFQLEYLSGGDVLKEMAMDEGFDSDGDDWWDTEEGMRFLNKREQDSKYDRALDEKLTLLFKKGGVVITSYTLPWLVTDGIKIWLEGSHESSTKRMQTRDNMSPQEAYEITKERFDKNKALYKKLYDFDFGEDKSVFDLIINTDHLTAQQVIDVAKETVRKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ15</accession>
    <name>A0A2S2KQ15_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">2-oxoacid:ferredoxin oxidoreductase subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_04850</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876354.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ15"/>
    <dbReference type="GeneID" id="76209837"/>
    <dbReference type="OrthoDB" id="31112at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016903">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000022">
        <property type="entry name" value="2-oxoglutarate ferredoxin oxidoreductase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022367">
        <property type="entry name" value="2-oxoacid/accept_OxRdtase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053400">
        <property type="entry name" value="2-oxoacid_Fdx_oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050722">
        <property type="entry name" value="Pyruvate:ferred/Flavod_OxRd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03710">
        <property type="entry name" value="OAFO_sf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041170">
        <property type="entry name" value="Oxoac_fdxalpha_Archa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154:SF16">
        <property type="entry name" value="PYRUVATE FLAVODOXIN_FERREDOXIN OXIDOREDUCTASE DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154">
        <property type="entry name" value="PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate/ketoisovalerate oxidoreductase catalytic" evidence="3">
        <location>
            <begin position="15"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate flavodoxin/ferredoxin oxidoreductase pyrimidine binding" evidence="4">
        <location>
            <begin position="254"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate:ferredoxin oxidoreductase core" evidence="5">
        <location>
            <begin position="521"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="636" mass="70660" checksum="B462038B0414760E" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ23</accession>
    <name>A0A2S2KQ23_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_05540</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829039.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ23"/>
    <dbReference type="GeneID" id="76209904"/>
    <dbReference type="OrthoDB" id="1036at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="58" mass="6550" checksum="CF1AB8839BFEB128" modified="2018-07-18" version="1">MGFKDWFVDDKTGNYSVKVQCTGCQRITVVKIPSGKTFEKWSEKIKCGQCNGSWIKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ39</accession>
    <name>A0A2S2KQ39_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL30-like ferredoxin-like fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_05150</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876377.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ39"/>
    <dbReference type="GeneID" id="76209866"/>
    <dbReference type="OrthoDB" id="6379at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.15.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004711">
        <property type="entry name" value="PRK06049.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="4">
        <location>
            <begin position="5"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="155" mass="17431" checksum="D53019395A929A27" modified="2018-07-18" version="1">MANAYLVVRIKGQADCPYWATHTMTLLKLDKKYRATILPAKENTLGMLRKVQHYVSWVELDASLAKELIDKKARKGGYQKITAEDLKELGFANSDELGTALAEGKATLSKLKPLKPWFALAPPRLGFKRSTKRLYGNKGILGQNKELDAIVRRMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ73</accession>
    <name>A0A2S2KQ73_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Probable transaldolase</fullName>
            <ecNumber evidence="5 12">2.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">tal</name>
        <name evidence="13" type="ORF">NZNM25_06130</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-erythrose 4-phosphate + beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:17053"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Carbohydrate degradation; pentose phosphate pathway; D-glyceraldehyde 3-phosphate and beta-D-fructose 6-phosphate from D-ribose 5-phosphate and D-xylulose 5-phosphate (non-oxidative stage): step 2/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the transaldolase family. Type 3B subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.2" evidence="5 12"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876463.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ73"/>
    <dbReference type="GeneID" id="76209967"/>
    <dbReference type="OrthoDB" id="6661at2157"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00414"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016832">
        <property type="term" value="F:aldehyde-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004801">
        <property type="term" value="F:transaldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00956">
        <property type="entry name" value="Transaldolase_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000018">
        <property type="entry name" value="Probable transaldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00494">
        <property type="entry name" value="Transaldolase_3b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001585">
        <property type="entry name" value="TAL/FSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022999">
        <property type="entry name" value="Transaldolase_3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004731">
        <property type="entry name" value="Transaldolase_3B/F6P_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018225">
        <property type="entry name" value="Transaldolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033919">
        <property type="entry name" value="TSA/FSA_arc/bac"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00875">
        <property type="entry name" value="fsa_talC_mipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683:SF40">
        <property type="entry name" value="FRUCTOSE-6-PHOSPHATE ALDOLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683">
        <property type="entry name" value="TRANSALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00923">
        <property type="entry name" value="TAL_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01054">
        <property type="entry name" value="TRANSALDOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00958">
        <property type="entry name" value="TRANSALDOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 12" id="KW-0570">Pentose shunt</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0704">Schiff base</keyword>
    <keyword evidence="7 12" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="12">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH33822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="222" mass="24094" checksum="6A545DF79F1231EA" modified="2018-07-18" version="1">MKIFLDTANLDSIKKFNDMGLLDGITTNPSLMSKEGGNPKNAMEEITKIIKGDVSLEVVSTEYSGMIEEGKRLREYGNNVVVKVPMTPDGLKACKSLSSEGIPVNVTLIFSANQALLAAKSGAKYVSPFIGRLDDVGQDGMKLIADIKEIFKNYPTFKTQILVASVRHPVHVVDAAKIGADVVTLPPAVLDKMLQHPLTKIGLENFLADWEKLKAGNPDVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ88</accession>
    <name>A0A2S2KQ88_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Methylmalonyl-CoA carboxyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_06410</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AccD/PCCB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876486.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ88"/>
    <dbReference type="GeneID" id="76209998"/>
    <dbReference type="OrthoDB" id="30579at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004658">
        <property type="term" value="F:propionyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000017">
        <property type="entry name" value="Propionyl-CoA carboxylase subunit beta 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000016">
        <property type="entry name" value="Propionyl-CoA carboxylase, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051047">
        <property type="entry name" value="AccD/PCCB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43842">
        <property type="entry name" value="PROPIONYL-COA CARBOXYLASE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43842:SF2">
        <property type="entry name" value="PROPIONYL-COA CARBOXYLASE BETA CHAIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="4">
        <location>
            <begin position="2"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="5">
        <location>
            <begin position="262"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="441"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="515" mass="56687" checksum="E10F7BFEC54D9E75" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQA2</accession>
    <name>A0A2S2KQA2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ATPase AAA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_06430</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876488.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQA2"/>
    <dbReference type="GeneID" id="76210000"/>
    <dbReference type="OrthoDB" id="8658at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389:SF6">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="34"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="385" mass="43422" checksum="463EF0F414502B02" modified="2018-07-18" version="1">MWSEKYRPQNISDMVGNEESRTAIMEWFAKWKKGTKPLLLVGPPGIGKTTIAYLVAKQFGYDMIGLNASDVRSKSRINEILTPVLGNVSVLGTPMIFVDEVDGIHGRGDYGGASALVDILKEPTVPIILAANNDTSDKMKSIKKVVKVISFKKIPPRLLRVYLENILKKENAKLSPGSLIKVIDKSKGDIRSMINFAQSLVTGFNPQTETTFENINVEDGVNAFFKANSIEEARVVLYSMQIDPREKINAFYSSIVSSTLDNSSLANYLETISNADMLFGKIMKTQNWRLLRYLNDILIKLYQNDDRIRYAQYNLSWPLLNRIRWDGAKIKSLSSIMAKKLHMSSSACVTLCLPYVLFCIKNKTLELELEETFGDIIEKEIELIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQD9</accession>
    <name>A0A2S2KQD9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA gyrase inhibitor YacG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_06910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQD9"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="53" mass="6006" checksum="EF15747E2A67F679" modified="2018-07-18" version="1">MGKFCKKCGRLMKNIDATHCSDECLLSDIRNSKPADESSKGIESWNDESDPWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQF7</accession>
    <name>A0A2S2KQF7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">3-isopropylmalate dehydratase large subunit</fullName>
            <ecNumber evidence="6">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Alpha-IPM isomerase</fullName>
            <shortName evidence="6">IPMI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">leuC_2</name>
        <name evidence="6" type="synonym">leuC</name>
        <name evidence="8" type="ORF">NZNM25_04760</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876345.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQF7"/>
    <dbReference type="GeneID" id="76209828"/>
    <dbReference type="OrthoDB" id="255at2157"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01583">
        <property type="entry name" value="IPMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01027">
        <property type="entry name" value="LeuC_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011826">
        <property type="entry name" value="HAcnase/IPMdehydase_lsu_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006251">
        <property type="entry name" value="Homoacnase/IPMdehydase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050067">
        <property type="entry name" value="IPM_dehydratase_rel_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033941">
        <property type="entry name" value="IPMI_cat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01343">
        <property type="entry name" value="hacA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02086">
        <property type="entry name" value="IPMI_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001614">
        <property type="entry name" value="PRK00402.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822:SF2">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="3 6" id="KW-0408">Iron</keyword>
    <keyword evidence="4 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="7">
        <location>
            <begin position="20"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="7">
        <location>
            <begin position="294"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="366"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="423" mass="45609" checksum="EBFE7261D46A365B" modified="2018-07-18" version="1">MNIAEKILARASGKSSVAPDDVVFANVDKVMVHDVSGPGVIKVFDKLKKQGISVDKLWDPTKVWVAEDHFVPSAEKVSAENIAKLSNFTKQYGIEKHFKYGMGQYGICHTISHEEAMVMPGDVYVGGDSHTNTTGALGAFACGLGHTDVAYVLLNGKIWFKVPETMYFKLNGKLPDHIMAKDLILKIIGDIGTDGGAYRTMQFGGSGIDAMSVESRLTMCNMTTEAGAKNGIVEPDQKVVDYLSSRGATNFNLIKGDDDAEYSKIFEYEASEMEPTIAKPFSPENVSIVRESPNVELDKSYIGSCTGAKYEDLEAAARILKGRTVKIRTEILPAAISIYKRAMENGLLKIFLDAGATVGPPTCGACCGAHMGVLAKDEICISTTNRNFPGRMGHVESQTYLASPLVAAASAVTGKITDPRDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQH1</accession>
    <name>A0A2S2KQH1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_07210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876586.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQH1"/>
    <dbReference type="GeneID" id="76208954"/>
    <dbReference type="OrthoDB" id="10607at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="215" mass="23839" checksum="A3FBB150E87DA40D" modified="2018-07-18" version="1">MRFTSSGNYDEFENDSREEAQLLEKFNDYANLEKKISKRAFLEISDESKPGKFGKKFIISAMVQGAIVTGLTIALFLNQLFVFSGNTQNMFDFAFTDNLGIFFFGFVLQIGLTAGLATIGIFYNHIETNLHNGFTKFNTLLSWIHLFGTNVFGSMITISLIFAGIATSPLYQTELSYLLSSIPALVYISAICLAVVMGIGILGTSFSFIKKNTDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQJ3</accession>
    <name>A0A2S2KQJ3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribosome biogenesis/translation initiation ATPase RLI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_06180</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876467.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQJ3"/>
    <dbReference type="GeneID" id="76209973"/>
    <dbReference type="OrthoDB" id="30658at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000152">
        <property type="entry name" value="ATP-binding cassette, sub-family E, member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001546">
        <property type="entry name" value="RNase L inhibitor homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013283">
        <property type="entry name" value="RLI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009945">
        <property type="entry name" value="PRK13409.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19248">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="Fer4_RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01868">
        <property type="entry name" value="ABCEFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="4"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="43"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="67"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="332"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="595" mass="66387" checksum="4C4DE88D707FBFCB" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQK0</accession>
    <name>A0A2S2KQK0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07270</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQK0"/>
    <dbReference type="OrthoDB" id="5029at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="143" mass="16663" checksum="236D3491BFE6E923" modified="2018-07-18" version="1">MYKNLETKSENIDNLFGEFIKPVSHKDKINFNKISINELINQSVENAINDVVSNTINTKIIITESENIREIAEKCKKFFQDSDFGTFEYIQRAGRSNFKVVHKSGINGTKFLKKFFEKMFTIHLKNYSIHTISNENSLCVIFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQN3</accession>
    <name>A0A2S2KQN3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein-glutamate methylesterase/protein-glutamine glutaminase</fullName>
            <ecNumber evidence="5">3.1.1.61</ecNumber>
            <ecNumber evidence="5">3.5.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cheB</name>
        <name evidence="8" type="ORF">NZNM25_07150</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in chemotaxis. Part of a chemotaxis signal transduction system that modulates chemotaxis in response to various stimuli. Catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins or MCP) by CheR. Also mediates the irreversible deamidation of specific glutamine residues to glutamic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-glutaminyl-[protein] + H2O = L-glutamyl-[protein] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16441"/>
            <dbReference type="Rhea" id="RHEA-COMP:10207"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="EC" id="3.5.1.44"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>[protein]-L-glutamate 5-O-methyl ester + H2O = L-glutamyl-[protein] + methanol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23236"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:10311"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:82795"/>
            <dbReference type="EC" id="3.1.1.61"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="5">Contains a C-terminal catalytic domain, and an N-terminal region which modulates catalytic activity.</text>
    </comment>
    <comment type="PTM">
        <text evidence="5">Phosphorylated by CheA. Phosphorylation of the N-terminal regulatory domain activates the methylesterase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CheB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.61" evidence="5"/>
    <dbReference type="EC" id="3.5.1.44" evidence="5"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876581.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQN3"/>
    <dbReference type="GeneID" id="76208960"/>
    <dbReference type="OrthoDB" id="2857at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008984">
        <property type="term" value="F:protein-glutamate methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050568">
        <property type="term" value="F:protein-glutamine glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16432">
        <property type="entry name" value="CheB_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17541">
        <property type="entry name" value="REC_CheB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.180">
        <property type="entry name" value="Methylesterase CheB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00099">
        <property type="entry name" value="CheB_chemtxs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008248">
        <property type="entry name" value="CheB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035909">
        <property type="entry name" value="CheB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000673">
        <property type="entry name" value="Sig_transdc_resp-reg_Me-estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001965">
        <property type="entry name" value="PRK00742.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42872">
        <property type="entry name" value="PROTEIN-GLUTAMATE METHYLESTERASE/PROTEIN-GLUTAMINE GLUTAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42872:SF6">
        <property type="entry name" value="PROTEIN-GLUTAMATE METHYLESTERASE_PROTEIN-GLUTAMINE GLUTAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01339">
        <property type="entry name" value="CheB_methylest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000876">
        <property type="entry name" value="RR_chemtxs_CheB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52738">
        <property type="entry name" value="Methylesterase CheB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50122">
        <property type="entry name" value="CHEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="10"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="CheB-type methylesterase" evidence="7">
        <location>
            <begin position="159"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="198"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="291"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="350" mass="38675" checksum="8D2E8A9009338E6C" modified="2018-07-18" version="1">MLKEETIPISVGIVNDSNLMRKYISDLINVDEIEIKWTAVNGEDALAKISKRKPDVILLDLEMPTMDGMTFIENMEKMKMMIPTIIVSSFSQDGSKVVLDALENGAVDFVTIPLKDIDDKKLQEELLTKIKISAKSDPIQLITEKIYSLKPSKKIFTKSNSSERLIVIGSSTGGPGMVQKVLSKLPANIAAGILVVQHMPKEFTKQFANRLSEKTGFIVREANEGDEIRDHEILVAPGDYHMIVLPNRRIHLDSSEKRFGVRPSVNMTMVSAAEVYGSNVIGVVLTGMGHDGGFGMKTIKKRGGHTIVQNKETCVIFGMPKAVIDLDAADHVVGINEIPIKIYEEMQNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQQ0</accession>
    <name>A0A2S2KQQ0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosomal protein eL8/eL30/eS12/Gadd45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_05660</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876422.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQQ0"/>
    <dbReference type="GeneID" id="76209918"/>
    <dbReference type="OrthoDB" id="10759at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039109">
        <property type="entry name" value="Ribosomal_eL30-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11449">
        <property type="entry name" value="RIBOSOMAL PROTEIN L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="3">
        <location>
            <begin position="5"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="107" mass="11891" checksum="63693CA0E2DF8A59" modified="2018-07-18" version="1">MSKILEKSLKDAHKENQLTMGTKQVLNSIKNSKLIVLSQSVNKEIIEKIESDAKKEKVPLVNFQGTSVALGRLCGLQFRISTISFTSITDANIKSILKDTEAEKKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQU3</accession>
    <name>A0A2S2KQU3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQU3"/>
    <dbReference type="OrthoDB" id="5725at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="96" mass="11151" checksum="EFC799E1DD7E939A" modified="2018-07-18" version="1">MQKIDYEGTVWVLNYNNPQPLLDHAIHMLERHGVKREDMIITETPTAKVGAIVVEIWPYELVIGRVRTTRNDSFISGTEFTIELKLDEDGNYIDYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQX4</accession>
    <name>A0A2S2KQX4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protoheme IX farnesyltransferase</fullName>
            <ecNumber evidence="11">2.5.1.141</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Heme B farnesyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Heme O synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ctaB</name>
        <name evidence="12" type="ORF">NZNM25_08440</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>heme b + (2E,6E)-farnesyl diphosphate + H2O = Fe(II)-heme o + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:28070"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.141"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Porphyrin-containing compound metabolism; heme O biosynthesis; heme O from protoheme: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cell membrane</location>
            <topology evidence="2 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="11">Carbon 2 of the heme B porphyrin ring is defined according to the Fischer nomenclature.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the UbiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the UbiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.141" evidence="11"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876691.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQX4"/>
    <dbReference type="GeneID" id="76208827"/>
    <dbReference type="OrthoDB" id="131615at2157"/>
    <dbReference type="UniPathway" id="UPA00834">
        <property type="reaction ID" value="UER00712"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008495">
        <property type="term" value="F:protoheme IX farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048034">
        <property type="term" value="P:heme O biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13957">
        <property type="entry name" value="PT_UbiA_Cox10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.140:FF:000006">
        <property type="entry name" value="Protoheme IX farnesyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00154">
        <property type="entry name" value="CyoE_CtaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006369">
        <property type="entry name" value="Protohaem_IX_farnesylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030470">
        <property type="entry name" value="UbiA_prenylTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01473">
        <property type="entry name" value="cyoE_ctaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003349">
        <property type="entry name" value="PRK04375.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448:SF2">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00943">
        <property type="entry name" value="UBIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 11" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH34053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="295" mass="32047" checksum="F5503F1718807C8F" modified="2018-07-18" version="1">MGFNEILEISKPRIVVLLVITAVTSMYAASKLVPGAPELDYWSYLHIIIAGALASAGSSALNHYYDKDIDPKMTRTSTRPIPSGRMTASKVLLYGIAVSCVSVIYGYFALNAVSAFFIAVGIFSYVIIYTVWLKRRNTSNIVIGGIAGSAASWAGWAAATGSMDLLGFLVGFLVFVWTPSHFWCLAMKIKDEYAQAGVPMLPVVIGMQKTSKFILGNTLILVPYSLILSLIPDGMGIVYTIIAIVSGGLMLVYHYKLTKNPTSDFAWKAYKVTAPYLTIIFVAVALDAAFHIPLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQX5</accession>
    <name>A0A2S2KQX5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_06490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876492.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQX5"/>
    <dbReference type="GeneID" id="76210006"/>
    <dbReference type="OrthoDB" id="10149at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="29"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="253" mass="27667" checksum="A872272760DEEB4A" modified="2018-07-18" version="1">MSEDKKESVDSSSDETSVKDTAVGVKIAEEAAAKAVESAEAAVEKVEEAKAAGETKAAEEAIAKAEAAAKAVESAEAAVKEALAKAEAAKAEAEAAAEEEYKAIAAEVKADAAKKSDYTFKRQGEEIFRREMGEPEFFLNKKDRFPRPILSQEEQESLTRQAEIPQDQTPVYVPEHVLSPEFGGVSNYERGVDSFLEESKIKLEKLKNNPDASEKELKDAENEIVYLESLHENFYIGMNVFRTAKGGRDKIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR14</accession>
    <name>A0A2S2KR14_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_07880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876643.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR14"/>
    <dbReference type="GeneID" id="76208888"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="224" mass="25913" checksum="9690290F19BA1427" modified="2018-07-18" version="1">MNPNLKNTLYAILSIMIIVPAILLIHDYIYFQEPLSRVSLYPDDPKCDKDCIEKVSDGYKCVEIKSDEFVCRLERGRHNFSDSAVFYSSAGPISYGEIVSFPEGKPDVYWFNVENMMILNKNSKTIQVDFYESKDNTSISNVIYTATLAPGDDYVSCVSPWKPGHLVRYIDLFEYENKTYAEFWGLHPFTPPELFPCDVQKILEYSLKTDYDISLPEYEEFGFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR25</accession>
    <name>A0A2S2KR25_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L15e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_09130</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR25"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1120.10">
        <property type="entry name" value="Ribosomal protein l15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024794">
        <property type="entry name" value="Rbsml_eL15_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000439">
        <property type="entry name" value="Ribosomal_eL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003269">
        <property type="entry name" value="PRK04243.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847">
        <property type="entry name" value="RIBOSOMAL PROTEIN L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00827">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01384">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="167" mass="19693" checksum="47FF1766FDD33491" modified="2018-07-18" version="1">MNNRFTVINGHFSMPDMPSHQDKTWIRLWNENAPELRERIVGWRKQNAITRIDKPSRIERARRLGYKAKQGIIVVRMRVGTGGMRKQRPTGGRRPKHLGVTRIKADDDMKTVANRRVLERYPNMKLLGSYFIYKDGMHYWFEVILADPQHPRIAQDKELNRQISQTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR67</accession>
    <name>A0A2S2KR67_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Blue (type 1) copper domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">NZNM25_07360</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="7">Binds 1 copper ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR67"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002386">
        <property type="entry name" value="Amicyanin/Pseudoazurin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000923">
        <property type="entry name" value="BlueCu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052721">
        <property type="entry name" value="ET_Amicyanin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507:SF1">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00127">
        <property type="entry name" value="Copper-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00155">
        <property type="entry name" value="AMICYANIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0186">Copper</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0574">Periplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Blue (type 1) copper" evidence="10">
        <location>
            <begin position="129"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="71"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602386-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH33945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="210" mass="22064" checksum="4F235B07D3652277" modified="2018-07-18" version="1">MPGIIMAGIDKAAIAFSIAIVAIGVGVAFSLGAAQDAVPIVSAPSMSTKTIEPKTQADPFADLAEKVKQEAPKVEEKKMEKPMEKSVEMEEKVEMEEIPKDETTMEKPAGPTTHTVNIPVGTSVPGCEESNTCFTPANITINAGDTVNWVNTDSAAHTVTGGSPADGPSGVFDSSLIMAGAEFAFTFNDSGNYDYFCMVHPWMVGSVSVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR86</accession>
    <name>A0A2S2KR86_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">AAA family ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_08420</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. CDC48 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876689.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR86"/>
    <dbReference type="GeneID" id="76208829"/>
    <dbReference type="OrthoDB" id="77269at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000007">
        <property type="entry name" value="AAA family ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000057">
        <property type="entry name" value="AAA family ATPase, CDC48 subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000189">
        <property type="entry name" value="AAA family ATPase, CDC48 subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001025">
        <property type="entry name" value="ATPase family, AAA domain-containing 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005938">
        <property type="entry name" value="AAA_ATPase_CDC48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01243">
        <property type="entry name" value="CDC48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="8">
        <location>
            <begin position="9"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48" evidence="7">
        <location>
            <begin position="109"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="213"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="486"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="722" mass="80265" checksum="DAF7EDB61867CC30" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRA0</accession>
    <name>A0A2S2KRA0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">MarR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829056.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRA0"/>
    <dbReference type="GeneID" id="76208809"/>
    <dbReference type="OrthoDB" id="7940at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052552">
        <property type="entry name" value="YeaO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36849:SF1">
        <property type="entry name" value="CYTOPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36849">
        <property type="entry name" value="CYTOPLASMIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22752">
        <property type="entry name" value="DUF488-N3i"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="120" mass="14383" checksum="81D3C06C780628E3" modified="2018-07-18" version="1">MAKVMIKIKRIYEDYSKTDGYRILVDRLWPRGITKERAKIDLWIKEITPSNDLRKWYHDNIEKREQFQKKYLVEIEKNSDSFNEIKKIINDKKTVTLLSASKDPKPIHAIVLQKTLTYKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRB8</accession>
    <name>A0A2S2KRB8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Roadblock/LAMTOR2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_10030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866853.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRB8"/>
    <dbReference type="GeneID" id="76208664"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046600">
        <property type="entry name" value="DUF6659"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20364">
        <property type="entry name" value="DUF6659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="14922" checksum="8D8B4F4AFF804036" modified="2018-07-18" version="1">MELKQICVIDDVCNQVRQIPGVRFVGIINKKGRKIAGGFSNKVSPLEKDEQKIEMLFMEIALDLSMRKDFDNSLGSINAIVSYRDKANIITIPYQENLILLSTEPELDTSKMIRIVQEKITFGKYIEVISQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRC3</accession>
    <name>A0A2S2KRC3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_10130</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutaredoxin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioredoxin family. DsbA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876834.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRC3"/>
    <dbReference type="GeneID" id="76208654"/>
    <dbReference type="OrthoDB" id="15256at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13887:SF14">
        <property type="entry name" value="DISULFIDE BOND FORMATION PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13887">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13462">
        <property type="entry name" value="Thioredoxin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="43"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="234" mass="25818" checksum="60B452BC68A63CBF" modified="2018-07-18" version="1">MGKRNKKTRQPGKSTTKFVMIGIICAVIAAVVGIAYVNLDAIKADNPNAVKLSLDTRSGSPVLGDMSAPVTIIEFGDYQCPFCKRWNESTKPAIEKDLIESGEASLIYIDFPIIGPDSLTIHAGSYCAQEQDLYWKYHDFVYANQGHENDGWANSENLKKLVSEIDGLDTNLFTQCLDSGKYEKRVEDNKKIASESGVRSTPTFIIIGPENAEMITGAQPYTTFKGIIDEMYGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRD0</accession>
    <name>A0A2S2KRD0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_10170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146195993.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRD0"/>
    <dbReference type="GeneID" id="76208650"/>
    <dbReference type="OrthoDB" id="6288at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="23211" checksum="97955FA25B60F66F" modified="2018-07-18" version="1">MKKYVFLFFLFVIISFPLFHSEVFGHGNPGIDRAPPIDFESRNVTVEARMNPSDMTVGDFSNAFMKITFLDVDTGKAFRQVTYSVDVYKKGELLARNNFYAEDGTTTIDIRPNSDCDKTPMWKCTKYYGIEHPIAGALYTFGQDNPVIDGPIFTNGGLYHIKVSVISADSVRSNLLHPLEFDLYVTIAQEQVFYITVPDHLIAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRG0</accession>
    <name>A0A2S2KRG0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Dimethyladenosine transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_10530</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876866.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRG0"/>
    <dbReference type="GeneID" id="76208609"/>
    <dbReference type="OrthoDB" id="9883at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000179">
        <property type="term" value="F:rRNA (adenine-N6,N6-)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727:SF7">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA adenine methylase transferase N-terminal" evidence="6">
        <location>
            <begin position="17"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="235" mass="26439" checksum="11BA694F9CD5D1CC" modified="2018-07-18" version="1">MIKRKRFGQHFLNSSSIAKSIVSEAKITKNDTVFELGTGLGILTPLLCEKANKVISMDVDKQLIEDAKSDFSKIHNLILKSGDGFKNNAPFTIFVSNLPYSKSKEAIEWLAESSFSHGVIMVQKEFAEKLLAKSSKKRKAISVIADHAFEISILSNVSKNNFSPPPKVDSVILKIVKKNIMKKDLIQTINKIFSYRRKTVKNILKQFNKETVIDKRVDDLSGDEIINLANQILEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRH6</accession>
    <name>A0A2S2KRH6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_10400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876858.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRH6"/>
    <dbReference type="GeneID" id="76208624"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="252" mass="28139" checksum="E5A5A515AA068B86" modified="2018-07-18" version="1">MLENDNGSFLKKNYPLVLIAVGFFLVLFPFNLFGTDKLGPISLVLYSLFAVLIMGFVLYYKQRSKYSAKTRKIVKLTALAIPLFFVASVIFGVALGIGNILYVSSLEPTKNLDRDLFEKEILDWTNTHRNQYGVGSVVLDPTLNDLAEIRSTNLSVAYPAYIEAYSDIDINVIAEREEIECIVNGTSVPIHDYTILVPSKSYSEMEKVVDLIMTYMVDDEGYGNIIFGSNITKTGIDTFVVGGDLFVVQNFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRI1</accession>
    <name>A0A2S2KRI1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_10500</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclic nucleotide phosphodiesterase class-III family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876918.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRI1"/>
    <dbReference type="GeneID" id="76208612"/>
    <dbReference type="OrthoDB" id="7513at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07400">
        <property type="entry name" value="MPP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050884">
        <property type="entry name" value="CNP_phosphodiesterase-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42988:SF2">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE CBUA0032-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42988">
        <property type="entry name" value="PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="248" mass="28225" checksum="743AB1B9B70A588A" modified="2018-07-18" version="1">MKIVQISDLHVGSQFLQDKFDTLVAEVNELNPEVIVITGDLTNEGLMKEYAQCKSMLEKFNTKKIIAISGNHDYRNTGYLLFKKFFPFESVNELNDDVVLVTVGTARPDRNEGEVGYRQNLWLERTMKKYKDKVKIVAMHHHLIAIPDTGSDQLTVVDAGDVLRTVLDTHVDIVLCGHKHRPWAWNFRTLTVVNAGTATSERVRGFFENTYNILTISDKKVQVDLKIVGGKRLPIDEIVNNYSQFADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRI7</accession>
    <name>A0A2S2KRI7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_10540</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876867.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRI7"/>
    <dbReference type="GeneID" id="76208608"/>
    <dbReference type="OrthoDB" id="7902at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.280">
        <property type="entry name" value="AF1531-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007003">
        <property type="entry name" value="DUF655"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40734:SF1">
        <property type="entry name" value="DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40734">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04919">
        <property type="entry name" value="DUF655"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160975">
        <property type="entry name" value="AF1531-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="188" mass="21489" checksum="EFACC5CF61156380" modified="2018-07-18" version="1">MYRAQSPPRKYEEHAYVLDFNPRGKSSTVRGREGIIVTAIGEDRLTLLEILGIPNSVFEIGEKIYIGKDGRTKVLSVLGKMDYDKISSSAQSELPAVVEKIVTNNESKFVEYLNKAQPLTPRIHALELIPGIGKTYMKTMLEEREKKKFESYDDLQERVGLKEPVKHISQRIMDEITGESRMNLFVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRJ7</accession>
    <name>A0A2S2KRJ7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ATP-dependent helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_10250</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829060.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRJ7"/>
    <dbReference type="GeneID" id="76208640"/>
    <dbReference type="OrthoDB" id="33870at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018973">
        <property type="entry name" value="MZB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47957">
        <property type="entry name" value="ATP-DEPENDENT HELICASE HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47957:SF3">
        <property type="entry name" value="ATP-DEPENDENT HELICASE HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09369">
        <property type="entry name" value="MZB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="3">
        <location>
            <begin position="197"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="4">
        <location>
            <begin position="409"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="880" mass="100118" checksum="98AD27660586224F" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRK2</accession>
    <name>A0A2S2KRK2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876950.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRK2"/>
    <dbReference type="GeneID" id="76208557"/>
    <dbReference type="OrthoDB" id="11405at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="213" mass="23716" checksum="7616131384B134D7" modified="2018-07-18" version="1">MEFLLENILNLVGFLVGLVIGIMSIIGFRNTGSPTLFRLSIAFLSISLGFFVIWMGYIIEDFVIKSGTIERWVQTLGIAIQTVGYFFIALSHSIKSFFPKSSYFRSVGILPLFLVSSVQLEHIFRSVSFILLAYGAIETMLSYFENKNKGAISVAVGLGLLALGEFLGWYSFVFPESILYTASMTIKIGGLVALFIPISKVPLTRIKFDDDFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRK7</accession>
    <name>A0A2S2KRK7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_09880</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876815.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRK7"/>
    <dbReference type="GeneID" id="76208679"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="108" mass="12339" checksum="3B935049B0C03B07" modified="2018-07-18" version="1">MKTDSRIIISIDKQFYSDDDEIRIHVWFNHAFYSPATLTVLSPTGRKIDSSGLKTDVNVTETFAFTCGGPFMFENGYHVIRVECENVVTEAMFEYYNSKNRFGSGFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRQ0</accession>
    <name>A0A2S2KRQ0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_09260</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRQ0"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010945">
        <property type="term" value="F:coenzyme A diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03426">
        <property type="entry name" value="NUDIX_CoAse_Nudt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045121">
        <property type="entry name" value="CoAse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992:SF11">
        <property type="entry name" value="MITOCHONDRIAL COENZYME A DIPHOSPHATASE NUDT8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992">
        <property type="entry name" value="NUDIX HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Nudix hydrolase" evidence="8">
        <location>
            <begin position="73"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="232" mass="26302" checksum="39022849A05E610A" modified="2018-07-18" version="1">MNFEIDSFVSTSGNIFGGFASYLIVMFSFLLHLVKTFEYQNPYEIVGKIEMNLDELKSVLSKPVNLKIDYDERYSLASVLVVIHGKEPQIVMTEKPKHMKFHAGEISFPGGKLEDADSDLLDTALRETSEEIGLHLSREQVIGQLEPVVTLNSGFLILPFISVINEIPPLLANPEVEKIFHIPLKSFLKTMSKDPDPSHNIIQEMYTFEYQNQIVWGASARILKQIRNLVGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRQ9</accession>
    <name>A0A2S2KRQ9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Argininosuccinate synthase</fullName>
            <ecNumber evidence="3 9">6.3.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Citrulline--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">argG</name>
        <name evidence="12" type="ORF">NZNM25_09360</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-citrulline + L-aspartate + ATP = 2-(N(omega)-L-arginino)succinate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10932"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 9">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the argininosuccinate synthase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.5" evidence="3 9"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876770.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRQ9"/>
    <dbReference type="GeneID" id="76208734"/>
    <dbReference type="OrthoDB" id="5877at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00113"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004055">
        <property type="term" value="F:argininosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000053">
        <property type="term" value="P:argininosuccinate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000050">
        <property type="term" value="P:urea cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01999">
        <property type="entry name" value="ASS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000019">
        <property type="entry name" value="Argininosuccinate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1260.10:FF:000007">
        <property type="entry name" value="Argininosuccinate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1260.10">
        <property type="entry name" value="Argininosuccinate synthetase, chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.470">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00005">
        <property type="entry name" value="Arg_succ_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048268">
        <property type="entry name" value="Arginosuc_syn_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048267">
        <property type="entry name" value="Arginosuc_syn_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001518">
        <property type="entry name" value="Arginosuc_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018223">
        <property type="entry name" value="Arginosuc_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023434">
        <property type="entry name" value="Arginosuc_synth_type_1_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024074">
        <property type="entry name" value="AS_cat/multimer_dom_body"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00032">
        <property type="entry name" value="argG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001770">
        <property type="entry name" value="PRK00509.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010392">
        <property type="entry name" value="PRK13820.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587">
        <property type="entry name" value="ARGININOSUCCINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587:SF2">
        <property type="entry name" value="ARGININOSUCCINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20979">
        <property type="entry name" value="Arginosuc_syn_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00764">
        <property type="entry name" value="Arginosuc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69864">
        <property type="entry name" value="Argininosuccinate synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00564">
        <property type="entry name" value="ARGININOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 9" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arginosuccinate synthase-like N-terminal" evidence="10">
        <location>
            <begin position="6"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Arginosuccinate synthase C-terminal" evidence="11">
        <location>
            <begin position="171"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="8"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF20979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH34145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="399" mass="44814" checksum="FD25066EC78DB1D4" modified="2018-07-18" version="1">MTQKGILAFSGGLDTSVVVKYLQEEHDMDVITVTVDVGQGDDAKKIAAKAKKLGVKKHYNIDARKEFVNKYIFRSIKANALYQKKYCLATALARPLIAEKVLEIAKKEKVTSLAHGCSGKGNDQVRFDITLRSGSDLPIIAPIRDKNLDRETELKFAKKHGIEIDAVAKRFSIDQNLWGRAIEGGVLEDPYNEPPDDAFIWVKTKNLPEKASYLEIKFESGIPVAVDGKKLESQKLIEYINKKAGNAGVGIVDHIEDRVVGIKSREVYETPAATCLIEAHSDLEKMVHTKHENKFKSLIDDEWAYLVYSGLWQDPLRRDLDGFIEESQKPVSGTVKLKMYKGSLRVVGRKSDFSLYSHEIATYGTESTFDQRLAKGFVELWGMQSTEANKLQKKRSTKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRY0</accession>
    <name>A0A2S2KRY0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_10060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRY0"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="61" mass="7001" checksum="ACC5326102E964DF" modified="2018-07-18" version="1">MQAATQCKAVITYKSFALIGRDAKNTLPIREEITYDAIGLSCLIRDEVLENYTYQTKRRCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRZ5</accession>
    <name>A0A2S2KRZ5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">5'-deoxynucleotidase</fullName>
            <ecNumber evidence="5">3.1.3.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_12170</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-phosphate + H2O = a 2'-deoxyribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:36167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18274"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:65317"/>
            <dbReference type="EC" id="3.1.3.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.89" evidence="5"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877064.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRZ5"/>
    <dbReference type="GeneID" id="76209287"/>
    <dbReference type="OrthoDB" id="46088at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002953">
        <property type="term" value="F:5'-deoxynucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3210.10:FF:000035">
        <property type="entry name" value="HD family hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039356">
        <property type="entry name" value="YfbR/HDDC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845:SF13">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13023">
        <property type="entry name" value="HD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD/PDEase" evidence="8">
        <location>
            <begin position="28"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="177" mass="20582" checksum="B69756EDE4869D08" modified="2018-07-18" version="1">MVVDFLKTAANLKKISRQGWIDKLSIDDSESVADHTYSMAIMGMVLSDLKNYDTEKILRMVLLHDLAESRIGDYTPDQLSKENKKELENNAFNEIIEKLPENIQSNYSEIWKEYQDGTSKESMLVHQIDRLEMTLQAKIYQKEGYSIDKLNSFFEYAKKDIVDPSLKELFTKIVDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS02</accession>
    <name>A0A2S2KS02_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_10260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876846.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS02"/>
    <dbReference type="GeneID" id="76208639"/>
    <dbReference type="OrthoDB" id="2830at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052048">
        <property type="entry name" value="ST_Response_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228:SF1">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ARR22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="1">
        <location>
            <begin position="4"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="131" mass="14771" checksum="97F0AFBF7815FB8D" modified="2018-07-18" version="1">MKPKVIIIDDDMESAEILSDLLSLRSIEVLGMGYDGQDAVKLYQKYSPDVVIMDYWMPDFDGLYGLENIRNLDSNAKVIILTGSPDNECNDDLFELKPSAIIQKPFDTNKLVELIDKISLGDIIQLDNKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS09</accession>
    <name>A0A2S2KS09_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Threonine synthase</fullName>
            <ecNumber evidence="5">4.2.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_11850</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the threonine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.1" evidence="5"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877037.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS09"/>
    <dbReference type="GeneID" id="76209321"/>
    <dbReference type="OrthoDB" id="6371at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004795">
        <property type="term" value="F:threonine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01563">
        <property type="entry name" value="Thr-synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000013">
        <property type="entry name" value="Threonine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF6">
        <property type="entry name" value="L-THREONINE DEHYDRATASE CATABOLIC TDCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="7">
        <location>
            <begin position="117"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="NCBIfam" id="TIGR00260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604450-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="445" mass="49120" checksum="14878C1ABB04DADE" modified="2018-07-18" version="1">MQGDAYLKCINPKCGLEYPVESTNVQCTNGHLLDVKYKKIPPTDLKEVFYKRRNSENSIFNESGVWRFRELLNFCQIDTENFEECSKYLVSLDGAEGRQSKPYQMSKAADFVGISTRNLWLQPEGYNPSGSFKDNGMATAVTHAKMVGAKKIVCASTGNTSASAGMFAANEGIDCDVYIPAGQIAPGKLSQAYQFGAQILEVNGNFDDALKQSLEDAQNHEGYTVNSVNPFRIEGQKTIPFRALEYLNWEVPDWIVYPGGALGNTSSCGKALMELYEWGWIKKIPRIAVINSDGASTLSDLYNGKFEDEEIRWNKGNPNTELISRYYDHLDKQGIKPKTKATAIQIGRPANILKGLRALEFTNGVVTTVSDSEMLDGMSVVGLNGFDCEMASGASVVGIKKLIAQEIIKKDDVVVGILTGRQKDAMLPVEYHQNPNNIFANPPKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS26</accession>
    <name>A0A2S2KS26_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 15 16">Dihydroxy-acid dehydratase</fullName>
            <shortName evidence="15">DAD</shortName>
            <ecNumber evidence="14 15 16">4.2.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ilvD</name>
        <name evidence="19" type="ORF">NZNM25_12470</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Functions in the biosynthesis of branched-chain amino acids. Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo-3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3-dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:24809"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:24810"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-oxopentanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:27694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="15">Binds 1 [2Fe-2S] cluster per subunit. This cluster acts as a Lewis acid cofactor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13 15">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12 15">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the IlvD/Edd family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.9" evidence="14 15 16"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877089.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS26"/>
    <dbReference type="GeneID" id="76209257"/>
    <dbReference type="OrthoDB" id="8674at2157"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00057"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00061"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004160">
        <property type="term" value="F:dihydroxy-acid dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.80:FF:000001">
        <property type="entry name" value="Dihydroxy-acid dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.80">
        <property type="entry name" value="IlvD/EDD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00012">
        <property type="entry name" value="IlvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050165">
        <property type="entry name" value="DHAD_IlvD/Edd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042096">
        <property type="entry name" value="Dihydro-acid_dehy_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004404">
        <property type="entry name" value="DihydroxyA_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020558">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056740">
        <property type="entry name" value="ILV_EDD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000581">
        <property type="entry name" value="ILV_EDD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037237">
        <property type="entry name" value="IlvD/EDD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00110">
        <property type="entry name" value="ilvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002068">
        <property type="entry name" value="PRK00911.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21000">
        <property type="entry name" value="DIHYDROXY-ACID DEHYDRATASE DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21000:SF5">
        <property type="entry name" value="DIHYDROXY-ACID DEHYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24877">
        <property type="entry name" value="ILV_EDD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00920">
        <property type="entry name" value="ILVD_EDD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143975">
        <property type="entry name" value="IlvD/EDD N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00886">
        <property type="entry name" value="ILVD_EDD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00887">
        <property type="entry name" value="ILVD_EDD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3 15" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 15" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7 15" id="KW-0408">Iron</keyword>
    <keyword evidence="8 15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroxy-acid/6-phosphogluconate dehydratase N-terminal" evidence="17">
        <location>
            <begin position="33"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Dihydroxy-acid/6-phosphogluconate dehydratase C-terminal" evidence="18">
        <location>
            <begin position="363"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="470"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="15">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="15">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="NCBIfam" id="TIGR00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF24877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="GBH34456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="557" mass="59382" checksum="8724469CEE743DD9" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS59</accession>
    <name>A0A2S2KS59_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877136.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS59"/>
    <dbReference type="GeneID" id="76209188"/>
    <dbReference type="OrthoDB" id="10488at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="111" mass="12647" checksum="2FB1164C36A8A6E6" modified="2018-07-18" version="1">MKIIDLHDPKRVNRPPDDVEILLSAGNFVHDEFLISKVELRLYNERLDSKLGVFSLITSFVETNKGSVEMIYDEGFRGENPLKRATEFLISNLGISGLILRSIISLREKIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS77</accession>
    <name>A0A2S2KS77_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">30S ribosomal protein S17e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_11060</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876955.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS77"/>
    <dbReference type="GeneID" id="76208552"/>
    <dbReference type="OrthoDB" id="52479at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.20">
        <property type="entry name" value="Ribosomal protein S17e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001210">
        <property type="entry name" value="Ribosomal_eS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018273">
        <property type="entry name" value="Ribosomal_eS17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036401">
        <property type="entry name" value="Ribosomal_eS17_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00833">
        <property type="entry name" value="Ribosomal_S17e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116820">
        <property type="entry name" value="Rps17e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00712">
        <property type="entry name" value="RIBOSOMAL_S17E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="61"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="81"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="98" mass="11488" checksum="3507FD34F8DB0456" modified="2018-07-18" version="1">MDRIKRLSYEVLVDHKSKFGEDFANNKKALDQISIIRSKGLKNEVAGYITKFIKKEIREEKFKQSRIESSRVEEQEDEEIQSQAPETVTEVTPEEKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSA9</accession>
    <name>A0A2S2KSA9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Prealbumin-like fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_13270</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146196000.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSA9"/>
    <dbReference type="GeneID" id="76209171"/>
    <dbReference type="OrthoDB" id="379933at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055890">
        <property type="entry name" value="DUF7467"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045826">
        <property type="entry name" value="SpaA_PFL_dom_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24269">
        <property type="entry name" value="DUF7467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19403">
        <property type="entry name" value="SpaA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7467" evidence="2">
        <location>
            <begin position="93"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="SpaA-like prealbumin fold" evidence="1">
        <location>
            <begin position="210"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="463" mass="50497" checksum="F9DF94745DB147EA" modified="2018-07-18" version="1">MTKLKTTLTISTIAAILIIGGFVSETPNAFSTYGGNHDNHDNYHYDDCDDRHHDKNRHDDCDNEPKDPCDCEKPDTLKFIFSTPSGEIDSEFKIEVYKKLNDIGNKDKKLMSIVGVTNLGDYQIESSNFGKDKLNSNTVIAIYKIVENQDDVLVSSMEIHTSCSQPLYKGLTVINSGYAVEITDGLKNSETSISESDPLTCEDDPVKMGSITIRKAITNDNGGDAAPADFTIMLTNVETNEEIILNHDQDESSDPLVNLNKVPVGTYKISESIADTVTATYTTVLITGDNQCPTMVEEEFTIKKGKSITCTIYNDDNGDGSGGPGGIVFQNNSMEINIEETAIDTLDSCDHYVNGIKTDPCIQIIGDSGNIGIVDSELSSTNTIVLFSVVEKTIDPDEGATNADCRLERIIAHDSTSDFLRDNDGNLPMNPTDNLMVFLGCSGIDSGKVYKINYVMIDPTMGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSC3</accession>
    <name>A0A2S2KSC3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_13440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877161.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSC3"/>
    <dbReference type="GeneID" id="76209155"/>
    <dbReference type="OrthoDB" id="11507at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="258" mass="29439" checksum="BCCA71A7D0DBBB1E" modified="2018-07-18" version="1">MAEPNTVPSKLRTNINYKVIAGIIFAVIVFHVYVNYFSNPEDSDSIISIFSFMNPLAVSIASFIVAIKYIDAGIYRKANISLGFAYLMVFLGELTYLIYDLFLDIDPYPSIADVFFFLQYPLTLTYVILNIRFFSSFSNKYKAWVILLPSIILTIYSINALNEYGEAGFDFYYGLIFVSSAGTLLAFSILGATIFREGLLGKAWMILVIGILTITIGDIWYYYLELIDEYDLSDPVNLFWYGGAWIIIYALYKHKKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSF5</accession>
    <name>A0A2S2KSF5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cysteine--tRNA ligase</fullName>
            <ecNumber evidence="12">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cysteinyl-tRNA synthetase</fullName>
            <shortName evidence="12">CysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12 15" type="primary">cysS</name>
        <name evidence="15" type="ORF">NZNM25_13830</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>tRNA(Cys) + L-cysteine + ATP = L-cysteinyl-tRNA(Cys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17773"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="12"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877196.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSF5"/>
    <dbReference type="GeneID" id="76209116"/>
    <dbReference type="OrthoDB" id="9445at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1910:FF:000026">
        <property type="entry name" value="Cysteine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000130">
        <property type="entry name" value="Cysteine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1910">
        <property type="entry name" value="Cysteine-tRNA ligase, C-terminal anti-codon recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015273">
        <property type="entry name" value="Cys-tRNA-synt_Ia_DALR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890:SF3">
        <property type="entry name" value="CYSTEINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="CYSTEINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09190">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00840">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 12 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Cysteinyl-tRNA synthetase class Ia DALR" evidence="14">
        <location>
            <begin position="342"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="403"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="12">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="12">
        <location>
            <begin position="261"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GBH34592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="462" mass="53348" checksum="3D9B39B7168569A9" modified="2018-07-18" version="1">MKIHNTLLNSEQELDISKKVKIYLCGVTVYDESHIGHARTIIIFDVLRKFLESKNVEIEFVQNFTDVDDKIINRANSENTTAEAISTKYIENYFKDFDGLNVKRATNYPKATEHIDDIIKFIEKLIEKKIAYTTKNGVYFSVSKFPEYGKLSKKKIDELQSGSRIEVDDAKNDPLDFAVWKFSSTEPVWDSPWGKGRPGWHIECSAMSIKYLGENFDIHGGGRDLIFPHHENEIAQSESCTGLPFAKIWMHVGMVTINGEKMSKSLGNIKSIKHVLENWGSNIIRLFCLSGHYSKPIDYSEELLKENVTKWRQVETSYYELIHAHGESDEKIDSIIYKTTTDFDDALNDDLNTHLALSAFFQLVKETNRLAAEEKLGSKDAQIIKKELERMMKILGLSIPEMTESEKQEINNLISNREKFRKDKQFEEADKIRDKLNDMNIELIDHKGKTVWMKKELILNDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSG6</accession>
    <name>A0A2S2KSG6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Bifunctional ligase/repressor BirA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">birA</name>
        <name evidence="3" type="ORF">NZNM25_13930</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877206.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSG6"/>
    <dbReference type="GeneID" id="76209106"/>
    <dbReference type="OrthoDB" id="46252at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin--[biotin carboxyl-carrier protein] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16442">
        <property type="entry name" value="BPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00978">
        <property type="entry name" value="Bifunct_BirA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030855">
        <property type="entry name" value="Bifunct_BirA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004408">
        <property type="entry name" value="Biotin_CoA_COase_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003142">
        <property type="entry name" value="BPL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013196">
        <property type="entry name" value="HTH_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00121">
        <property type="entry name" value="birA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835">
        <property type="entry name" value="BIOTIN PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835:SF5">
        <property type="entry name" value="BIOTIN--PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02237">
        <property type="entry name" value="BPL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08279">
        <property type="entry name" value="HTH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="2">
        <location>
            <begin position="76"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="335" mass="37242" checksum="FE017B1AFEBF7B9A" modified="2018-07-18" version="1">MIYNSFDSPGLVKVLTFLQTHNTEYLSGQDLSDVLRISRVAVWKHIKKIQELGYTVESKQKLGYKLTSNSDALLPWEITAGLKTKKIGKKAYYFDSIDSTQNQALKMSEESVNDGTIIIAAKQTGGKGRLGRKWISPKGGIWFSIILQPKFDISITTLFPIASSLALSYAIEKTFKISPELKWPNDLTIKGKKVAGMLVDVSLESNKIESLVLGVGINFDVDTKQIEKTLKGTSNFYGVASLNEQKMKVKPKVLIQTFLVELEKIYELLNSKQTKNIIAEWTKRSSTIGKKVELNTVEGKIKGDAIKIDDDGALIVSEKNKISKIIAGDIIHLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSI0</accession>
    <name>A0A2S2KSI0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribbon-helix-helix protein, CopG family</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13650</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSI0"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="22678" checksum="790EF4EB224459A0" modified="2018-07-18" version="1">MDSNTIKSQQHPTKKEKTRSVTYRLPVKLVEEIEAEAMNRNISHNVMARQILEKYIRWDRFTEKIGIIPVPKKILDILGTEMSSKDINEIINVIKPVIKDTVLFIKGGYDLKRCIETLEDYMRASGMKSDHRIEGASHHFVIQHELGSNWSRFTEQLLGEIFHEFLPDTRMKSQTTESTVVATIKLGEDFMEHDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSI2</accession>
    <name>A0A2S2KSI2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13900</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877203.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSI2"/>
    <dbReference type="GeneID" id="76209109"/>
    <dbReference type="OrthoDB" id="26482at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="120" mass="13989" checksum="CBB7BC9526C02BE7" modified="2018-07-18" version="1">MTFENFYHDLLDLAKKYEKQNIPLKIEPDLENNIVKIFGEKITSLTRAQNGLHDVAELAYATAEHHPYWNILYNSSEIANSVLDKWQSSLSEDDLSDIDWALKEMEKTLEKIKNQKHSHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSP4</accession>
    <name>A0A2S2KSP4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_13580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_263970075.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSP4"/>
    <dbReference type="GeneID" id="76209141"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="44" mass="5272" checksum="072304DA3A941236" modified="2018-07-18" version="1">MTKSKNKNKTQINKNNKTTPDNLKKKQYLESYEELKKSIENDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KST4</accession>
    <name>A0A2S2KST4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">4Fe-4S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_15310</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007403030.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KST4"/>
    <dbReference type="GeneID" id="56064006"/>
    <dbReference type="OrthoDB" id="5583at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050572">
        <property type="entry name" value="Fe-S_Ferredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687">
        <property type="entry name" value="ADENYLYLSULFATE REDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687:SF6">
        <property type="entry name" value="L-ASPARTATE SEMIALDEHYDE SULFURTRANSFERASE IRON-SULFUR SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="5"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="44"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="100" mass="11039" checksum="735775CCD21D310A" modified="2018-07-18" version="1">MPIAILPDIDEQRCIGCALCVEICTTLGPDVLRVKPVEGWKRGKAFVFYPERCISDGACIGVCPTKSIFWMRPMNYTAGQPVPLHKNGIFIKGWAEDAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSV1</accession>
    <name>A0A2S2KSV1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_14180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877225.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSV1"/>
    <dbReference type="GeneID" id="76209077"/>
    <dbReference type="OrthoDB" id="12071at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="129" mass="13932" checksum="18A99AB3B935EB73" modified="2018-07-18" version="1">MNKLNILFFAAAVTTAIAGIIHVVLGIPSNNLNSQVLFVVGGATQIFWAVPMIRKWGTVWYGIGLGGTLMFIAIWVITRIPDNFITGRGGRISDNGILVEAFQIAFVGIVIAILVLDRKSFSKKEGNAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSV4</accession>
    <name>A0A2S2KSV4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed RNA polymerase subunit Rpo6</fullName>
            <ecNumber evidence="3">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">DNA-directed RNA polymerase subunit K</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpo6</name>
        <name evidence="3" type="synonym">rpoK</name>
        <name evidence="5" type="ORF">NZNM25_13160</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal Rpo6/eukaryotic RPB6 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSV4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00192">
        <property type="entry name" value="RNApol_arch_Rpo6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020708">
        <property type="entry name" value="DNA-dir_RNA_polK_14-18kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006110">
        <property type="entry name" value="Pol_omega/Rpo6/RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036161">
        <property type="entry name" value="RPB6/omega-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006111">
        <property type="entry name" value="Rpo6/Rpb6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002207">
        <property type="entry name" value="PRK01099.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002208">
        <property type="entry name" value="PRK01099.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47227">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47227:SF5">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01192">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01409">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63562">
        <property type="entry name" value="RPB6/omega subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01111">
        <property type="entry name" value="RNA_POL_K_14KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="208" mass="23854" checksum="825151A7134A2B73" modified="2018-07-18" version="1">MPRISKISCFFETEGELNSYIKMIRLYNVLRKFCYLSDVEEAPLLESPESEEPFLESTESEEIEVSPEENTGLNKALDTYRKLIEKKGGLEPLSEKEQENLEKRIKEIESREVVETVEEHEATEIPCEKGKITIGPPTLTRFEKARIMGARALQLSLGAPPFIPIPKTARISLDIAMEELEQRVIPITIRRVLPNGDFQNIPIDYFEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSX6</accession>
    <name>A0A2S2KSX6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_14620</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877353.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSX6"/>
    <dbReference type="GeneID" id="76209033"/>
    <dbReference type="OrthoDB" id="342253at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009927">
        <property type="term" value="F:histidine phosphotransfer kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06225">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00075">
        <property type="entry name" value="HATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000006">
        <property type="entry name" value="Sensor histidine kinase WalK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047:SF72">
        <property type="entry name" value="OSMOSENSING HISTIDINE PROTEIN KINASE SLN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158472">
        <property type="entry name" value="HAMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="9">
        <location>
            <begin position="337"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="404"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="632" mass="70603" checksum="F30E9BA61748D253" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT25</accession>
    <name>A0A2S2KT25_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_14980</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866905.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT25"/>
    <dbReference type="GeneID" id="76208997"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052968">
        <property type="entry name" value="Nucleotide_metab_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42146">
        <property type="entry name" value="3',5'-CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42146:SF1">
        <property type="entry name" value="OLIGORIBONUCLEASE NRNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDH" evidence="1">
        <location>
            <begin position="48"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="2">
        <location>
            <begin position="284"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="368" mass="40615" checksum="C78F9D0914832200" modified="2018-07-18" version="1">MTVSKTKKHTTKKIVKKTVTKKVSKKINKKPTKKITATRTVKPQRTKVICISHKEDCDGISSAALIRQAFGGDAILVDYPGQMEALNQVVLDEKLKSLFICDLGLSKKTQDEFIDIMTTLRKNKVSVTYIDHHDIDPNVLKALNKIKVKIIHDTNECTAVLVYNAFKSKLNDHASFVAACAAITDYMETRPIGAKLLQIYDRQFALISATVLTYNIVGHQKEPDYLLYLVEELADSKFPHAIPNTFEFAQIQVEKLSQMIAKVKQGLKTMKNLGYMEILDAGASGAVNFVMGLSGKNVGVAYKERVDHGIYAVSVRGSKDCKVHLGKIVNILATNLGGSGGGHDKACGAVIPKPKIKKFITELNKQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT29</accession>
    <name>A0A2S2KT29_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Small ribosomal subunit protein eS19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rps19e</name>
        <name evidence="6" type="ORF">NZNM25_16210</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May be involved in maturation of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eS19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877457.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT29"/>
    <dbReference type="GeneID" id="76208365"/>
    <dbReference type="OrthoDB" id="371836at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000449">
        <property type="entry name" value="30S ribosomal protein S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01474">
        <property type="entry name" value="Ribosomal_eS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001266">
        <property type="entry name" value="Ribosomal_eS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027548">
        <property type="entry name" value="Ribosomal_eS19_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006811">
        <property type="entry name" value="PRK09333.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01090">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01413">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="150" mass="16910" checksum="5B3FE9D290E38005" modified="2018-07-18" version="1">MAKVYDVPADVLIKRLANILKNEDIPAPSWIPFVKTGAHADRPPQERDWWYTRCASIMRKIYFHGPIGINELRKDYGGGKPSGYGAAHHKDAGGAIIRNAIQGLEKLGYLEKVEKKGRIISKNGMQKLDRLSTEILKELIVENPQLKVYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT31</accession>
    <name>A0A2S2KT31_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_14090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829086.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT31"/>
    <dbReference type="GeneID" id="76209090"/>
    <dbReference type="OrthoDB" id="3221at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="41751" checksum="BD06A5DE69CD02F5" modified="2018-07-18" version="1">MKTVIWGILGICIIFSFNAQSAFAHTPDFEITTTKDILKFCEFFYEEYQLLGIYDLTLQHPQFPNLRACAILYNHVAWNSTHEARNVVLIAEIEKYLGDSSYIKDRHIVISDIIPDWAKREARLWVNGENQDVGFAYVVRTMLEAGILKLDFIEKNCDGDEICMKEGDFIKYSHFDKFGNISTIKHTVKSIINEEITLTVEKTSDEGKIKEEITLEKNGLIKNDECCKYYEFVIPTQISLGDKISNNLKIVSETTHIFENQMKQSWYATDTTGQNTKVIDKNTGLVFLYEHHETTVLSVGEKTKITDTNFFDSKYNVEEHQIKIPDWLKKTTAWLLDKRIPEEEYLRAIENLISRNIIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT46</accession>
    <name>A0A2S2KT46_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tetratricopeptide repeat protein 21A/21B N-terminal ARM repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_15180</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877312.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT46"/>
    <dbReference type="GeneID" id="76208978"/>
    <dbReference type="OrthoDB" id="115601at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056833">
        <property type="entry name" value="ARM_TT21_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047558">
        <property type="entry name" value="TPR_END_plus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF8">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25062">
        <property type="entry name" value="ARM_TT21_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13174">
        <property type="entry name" value="TPR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="137"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="204"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Tetratricopeptide repeat protein 21A/21B N-terminal ARM repeat" evidence="4">
        <location>
            <begin position="234"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="238"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="272"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="475"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="600" mass="68262" checksum="14A1ED2EB40A9FDC" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT60</accession>
    <name>A0A2S2KT60_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_16170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877453.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT60"/>
    <dbReference type="GeneID" id="76208369"/>
    <dbReference type="OrthoDB" id="2961at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="203" mass="23209" checksum="A4966E07148885A1" modified="2018-07-18" version="1">MGGIDRLISNALLSEIKKKMDLELLHKVERELFLEHGMSIKLSIEHFQKFTSVLKKNSSLDVSKFERDCLDKIIRIKESNEKYFVTIINPILSDSILELFGEYDSRCIITTLLDKEYTIPQILKESKAPKTSGYRKIENMMINGLIVETGKVLSESKKISKLQCVFQEINLDIKKGKTVVNGIIPKKIMEKSSSVKSILQTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT82</accession>
    <name>A0A2S2KT82_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_16450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877471.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT82"/>
    <dbReference type="GeneID" id="76208340"/>
    <dbReference type="OrthoDB" id="11724at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="212" mass="22599" checksum="9605A116EBB7BE22" modified="2018-07-18" version="1">MISVKLVIFGFIGLLFSIGMIDASFAHTTVEVEQYHIEAGWGIEPPVVGIRNDIVFKITEPGETEGSYKGVTNVFRNVEATAMYGGAAKKIDINSDPRPGYYFSPIIPTKTGGYIMNLQGDIRGTIIDIKIPVEDVEPTAVLDFPPTSNEGTADITALKNALLSLQQDVSKLQSGETSVSSDGGAAYDFAIFGLSIAAAAIVLAIIALVKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT83</accession>
    <name>A0A2S2KT83_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_16370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877466.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT83"/>
    <dbReference type="GeneID" id="76208348"/>
    <dbReference type="OrthoDB" id="11784at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="421" mass="48406" checksum="1CAF2D7D40A8516E" modified="2018-07-18" version="1">MKNQHPDLKISSTLSNSIFCISTIVCFCLVLFSPVAHADVFIPDNEYHGYYDFEGIYTVVGNVKNQNDFALIPTITISVIDNETKITKTLNHVPIPPMVDIPFKLKFPEIHGQNPVVLKAELKYVKTDKPPIPIQIIYDKTLITYDDGHVTGRIKNIGNQTVYNPKIFAIVHGYEKYILDVAQNIDRIEKIEPGEILNFTIYPDPSVSEPVRFYSCFAPVDTTVIPLTTKKNNGDFDFRYDSGAWYYAAKFDEKGTTLNIKGYNSYPLETYANFEFPPISGNEKFTVTLNDESIEFIQSVDEMGFWHVAFKVESRSQGVLKISGFEKGLPPELPKIPQWIKVNTEWWITNQISDLEFLEGIDFLFEKQIISVPERDVIAESQWKIPEWVKISAEWWYEEKISDDDFLNTIENLVKRKIIVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT87</accession>
    <name>A0A2S2KT87_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_15820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT87"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="74" mass="8290" checksum="EFD04B87CD3D2F45" modified="2018-07-18" version="1">MLIPVNAFTLYPSSASFTSIVKRSINFLLTNLLIREYTTDEFTLIFFDNLGTEIDASLDNSDKICKSKSSTFAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT93</accession>
    <name>A0A2S2KT93_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Aspartate carbamoyltransferase regulatory chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pyrI</name>
        <name evidence="10" type="ORF">NZNM25_16540</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Involved in allosteric regulation of aspartate carbamoyltransferase.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Contains catalytic and regulatory chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the PyrI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877480.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT93"/>
    <dbReference type="GeneID" id="76208331"/>
    <dbReference type="OrthoDB" id="7000at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009347">
        <property type="term" value="C:aspartate carbamoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.20">
        <property type="entry name" value="Aspartate carbamoyltransferase regulatory subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.140">
        <property type="entry name" value="Aspartate carbamoyltransferase regulatory subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00002">
        <property type="entry name" value="Asp_carb_tr_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020545">
        <property type="entry name" value="Asp_carbamoyltransf_reg_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002801">
        <property type="entry name" value="Asp_carbamoylTrfase_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020542">
        <property type="entry name" value="Asp_carbamoyltrfase_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036792">
        <property type="entry name" value="Asp_carbatrfase_reg_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036793">
        <property type="entry name" value="Asp_carbatrfase_reg_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00240">
        <property type="entry name" value="ATCase_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35805">
        <property type="entry name" value="ASPARTATE CARBAMOYLTRANSFERASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35805:SF1">
        <property type="entry name" value="ASPARTATE CARBAMOYLTRANSFERASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01948">
        <property type="entry name" value="PyrI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02748">
        <property type="entry name" value="PyrI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57825">
        <property type="entry name" value="Aspartate carbamoyltransferase, Regulatory-chain, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54893">
        <property type="entry name" value="Aspartate carbamoyltransferase, Regulatory-chain, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aspartate carbamoyltransferase regulatory subunit N-terminal" evidence="8">
        <location>
            <begin position="6"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartate carbamoyltransferase regulatory subunit C-terminal" evidence="9">
        <location>
            <begin position="102"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="153" mass="17441" checksum="120B37AE16D8FF8B" modified="2018-07-18" version="1">MEQSELMVRRIKEGTVIDHIDGGKGLQVLSALRIDGRDGELITIALNVPSGKFKKKDIIKIENKFLKDDDTNKLAVIAPKATINMIKDFKLVEKRRVSLPNEIDRIFRCSNPDCITNSTEHIESIMDVIDKEGRVLKCRYCARVLDVNKLKYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTC4</accession>
    <name>A0A2S2KTC4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_14990</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTC4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="63" mass="7318" checksum="4B23A8E3A64C65ED" modified="2018-07-18" version="1">MTPKERKDLKKLDDLVLNASSDELKKIQEIDHQTQLEGLSFFEVYVDSSSLIHKNIKKSSRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTF2</accession>
    <name>A0A2S2KTF2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nucleotide-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_16280</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877462.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTF2"/>
    <dbReference type="GeneID" id="76208357"/>
    <dbReference type="OrthoDB" id="9573at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.30.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002878">
        <property type="entry name" value="ChsH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022002">
        <property type="entry name" value="ChsH2_Znr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052513">
        <property type="entry name" value="Thioester_dehydratase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075:SF5">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01796">
        <property type="entry name" value="OB_ChsH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12172">
        <property type="entry name" value="zf-ChsH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ChsH2 rubredoxin-like zinc ribbon" evidence="2">
        <location>
            <begin position="11"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="ChsH2 C-terminal OB-fold" evidence="1">
        <location>
            <begin position="48"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="13301" checksum="1B94F47F9C54184C" modified="2018-07-18" version="1">MTIEEQLIEYAKQGKLLTHKCTKCGYLHLSTAYYCLKCGSQGFEDVILDGVGSIATYTIITVAPAGFEKYTPYAFVVLQLDNTDLRISGFMAGIATPDDLPIGTRAHITGFEDGCGIIIKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTK2</accession>
    <name>A0A2S2KTK2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Probable cobyric acid synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cobQ</name>
        <name evidence="9" type="ORF">NZNM25_15790</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the CobB/CobQ family. CobQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877407.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTK2"/>
    <dbReference type="GeneID" id="76208408"/>
    <dbReference type="OrthoDB" id="53136at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05389">
        <property type="entry name" value="CobQ_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00028">
        <property type="entry name" value="CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047045">
        <property type="entry name" value="CobQ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004459">
        <property type="entry name" value="CobQ_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001989">
        <property type="entry name" value="PRK00784.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21343:SF1">
        <property type="entry name" value="COBYRIC ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21343">
        <property type="entry name" value="DETHIOBIOTIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="8">
        <location>
            <begin position="4"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="279" mass="31031" checksum="34EC71A6ECFFD234" modified="2018-07-18" version="1">MKSLMIQGTSSGAGKTTLVAALCRIFSDKGYNVAPFKSQNMSNFGYVTPTFEISRAQAIQAIGARCTITPDLNPILLKPIGNYNSIVYLHGKRYKKMHAKDYYEKFVNTKGFNIASRSLKTLQKNFDLVILEGAGSPAEINLQKFDIANMRIAERAKASVLLVSDIDKGGSFASIVGTMALIEKKYQQLVKGFIFNKFRGDIDVLKPGFQKLKKLTTIPVVGTVPLISLDLPEEDSLHAKPKDITWNKKNLLKIDNELNRLAKTVKSNIDIKSIERMLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTM2</accession>
    <name>A0A2S2KTM2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="1">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_17830</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="1"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877588.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTM2"/>
    <dbReference type="GeneID" id="76210338"/>
    <dbReference type="OrthoDB" id="12184at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00317">
        <property type="entry name" value="cyclophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.10">
        <property type="entry name" value="Mog1/PsbP, alpha/beta/alpha sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049886">
        <property type="entry name" value="CFI_box_CTERM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002683">
        <property type="entry name" value="PsbP_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041770">
        <property type="entry name" value="CFI_box_CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF4">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE DOMAIN AND WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01789">
        <property type="entry name" value="PsbP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0697">Rotamase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="484"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="5">
        <location>
            <begin position="32"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="515" mass="57376" checksum="D164E0E6F25003CF" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTM4</accession>
    <name>A0A2S2KTM4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine hydroxymethyltransferase</fullName>
            <shortName evidence="9">SHMT</shortName>
            <shortName evidence="9">Serine methylase</shortName>
            <ecNumber evidence="9">2.1.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">glyA</name>
        <name evidence="12" type="ORF">NZNM25_17700</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the reversible interconversion of serine and glycine with a modified folate serving as the one-carbon carrier. Also exhibits a pteridine-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SHMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.-" evidence="9"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877576.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTM4"/>
    <dbReference type="GeneID" id="76208215"/>
    <dbReference type="OrthoDB" id="5821at2157"/>
    <dbReference type="UniPathway" id="UPA00288">
        <property type="reaction ID" value="UER01023"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000101">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000114">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF35">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5 9" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8 9 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="11">
        <location>
            <begin position="12"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="123"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <feature type="site" description="Plays an important role in substrate specificity" evidence="9">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9 10">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH34979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="440" mass="48884" checksum="AADB0A86FC71D3F9" modified="2018-07-18" version="1">MAKSQNREAYDKIFTKLKEHHKWFENSIPLIASENIPSPAVREAIISDFGNRYAEGWPGERVYAGCVYIDEVEFECMKLAKKLFKAKFADVRPISGVVANLAIYSAFTNPGDIMLAPSIPAGGHISHGKKEHFGTAGLVHGLEIEFYPFDAEEMTIDVDKTKQKIMELKKDSRLPKMAMFGGSLFLFPHPVKELSDFLKSYDIHINYDAAHVAGLIAGGKFQDPLREGADTMTMSTHKTLFGPQGGLVLGSEKHEEVIKKATFPGLTSSHHIHHMAGKAVTFAEALEFGKDYASAVIKNAKSLADALSDMGFKVLGENKGFTKSHQIAVNVLDYSDGGKVESDLEKANIIVNRQLIPGDIKAGRNYFHPGGIRLGVSEITRLGMKKNEMEEIASLIKNIVIEKKDPKKILSKVKSFRKNFQKVKFCFDNKLGAYEYVKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTP4</accession>
    <name>A0A2S2KTP4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ATP-cone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_16260</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTP4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031250">
        <property type="term" value="C:anaerobic ribonucleoside-triphosphate reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008998">
        <property type="term" value="F:ribonucleoside-triphosphate reductase (thioredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009265">
        <property type="term" value="P:2'-deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055771">
        <property type="entry name" value="DUF7347"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012833">
        <property type="entry name" value="NrdD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21075">
        <property type="entry name" value="ANAEROBIC RIBONUCLEOSIDE-TRIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21075:SF0">
        <property type="entry name" value="ANAEROBIC RIBONUCLEOSIDE-TRIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24038">
        <property type="entry name" value="DUF7347"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13597">
        <property type="entry name" value="NRDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-cone" evidence="4">
        <location>
            <begin position="156"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="736" mass="81485" checksum="4B518799F3CFF2D7" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTP8</accession>
    <name>A0A2S2KTP8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acetyl-CoA acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_16290</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877463.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTP8"/>
    <dbReference type="GeneID" id="76208356"/>
    <dbReference type="OrthoDB" id="167534at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00829">
        <property type="entry name" value="SCP-x_thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055140">
        <property type="entry name" value="Thiolase_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004720">
        <property type="entry name" value="PRK06064.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870:SF6">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22691">
        <property type="entry name" value="Thiolase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="2">
        <location>
            <begin position="8"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="3">
        <location>
            <begin position="250"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="387" mass="40801" checksum="7E404D38DA82E6CA" modified="2018-07-18" version="1">MTFVEKVCVLGAGSTKYGKLSESIADITTQASVAAIESAGISPKDIKASYISNVFGVADKQVHIGPVLMSRLGIPDKPSLTIESACGSGSVSFREAYANVAAGFYDCLVVTGVEKVTHTGTEWTTTYFAYCSDFFYEGQAGASFPGLFASMARAYLTEFNATEEDFAAVAVKNHDNGFLNPKAHMQKKITIDDVMNSAVVASPLKLYDCCPFSDGASSVILCSEKFAKEHGGDYIEVIGSGRGGSPAALQGREHMTTIPSTKIAAADAYKMAGITTKDIDFAEVHDCFTIAEVVDTEDLGFFEKGQGIQAVREGRTKLNSDISINPSGGLKSKGHPIGATGIGQVVEVFDQLTGKAGERTVKDAKIGLTHNFGATGASCAVHIFQSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTQ8</accession>
    <name>A0A2S2KTQ8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_16390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877650.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTQ8"/>
    <dbReference type="GeneID" id="76208346"/>
    <dbReference type="OrthoDB" id="3154at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="130" mass="14341" checksum="BFE30446E9FB2072" modified="2018-07-18" version="1">MFRPERIVERKSTLFSIVVTGVIAILALPIIIPHLLHGYHLVHIFLHVGGITLAVFISVLAGAAYYRLRTKRLLLSAAAFTNFIAAEVVLLFDATWPNIYNLGGMSFPEVGHLLTFITLGLLALGVFRND</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTT6</accession>
    <name>A0A2S2KTT6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_17820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877587.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTT6"/>
    <dbReference type="GeneID" id="76210339"/>
    <dbReference type="OrthoDB" id="8874at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="163" mass="19744" checksum="7B57D5C2727BDF07" modified="2018-07-18" version="1">MQPDEKIQAHLVSVWRESKKFFSIGGKEGMLILTDKHLMFIHKTESKMNWWKAITQRQVVNFIKSKNTMIRHDGYDEKELMNDLEDERNIELSFDDIYNISYEEKDWGTPLLIEYEKDGKQEKFQYSIAQDWVKYPVKEPMKYMKVDWKPFVQYIKDGQKFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTW0</accession>
    <name>A0A2S2KTW0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cell division protein SepF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_16960</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877515.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTW0"/>
    <dbReference type="GeneID" id="76208290"/>
    <dbReference type="OrthoDB" id="6148at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.150">
        <property type="entry name" value="SepF-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007561">
        <property type="entry name" value="Cell_div_SepF/SepF-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038594">
        <property type="entry name" value="SepF-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04472">
        <property type="entry name" value="SepF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="92" mass="10602" checksum="6F8D16DD31E1D7AC" modified="2018-07-18" version="1">MQKQESPTYLKAITIRDISDVHSIKEDIKKEMILILRVTPLAQKDVEQLRKVVEELYSIAKAADADIARLGEERIIVAPSNIKIWKPEYDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTW4</accession>
    <name>A0A2S2KTW4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_18120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877613.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTW4"/>
    <dbReference type="GeneID" id="76210309"/>
    <dbReference type="OrthoDB" id="9963at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="64" mass="6878" checksum="AD74A4CB8BFD6AEF" modified="2018-07-18" version="1">MASKSITVGVGIPMIIVGALMAWLWAPLEGVFQNQVELIGSTIGILGVVFFISGLFYTKQPVMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTY8</accession>
    <name>A0A2S2KTY8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_18180</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTY8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010340">
        <property type="entry name" value="PRK13768.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF8">
        <property type="entry name" value="GPN-LOOP GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="11"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH35027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="263" mass="28817" checksum="518E6DDE58F50DDC" modified="2018-07-18" version="1">MKYKQIKNQTGLKTIFISGTAGSGKSLLTSKLHDYYTKNGAFVAVLNLDPGVENIPYTCDVDVRDYVDIVSIMHQYDLGPNGAMIMANDLIASKIDDIQNEVNRINPDYLIVDTPGQIELFAYRSSGRFLVENISSDEKTNIFLFDGALVTTPVNFVSIALLATSIRLRLNLSTINVVTKIDLIQDKLKEILQWSTNLNTLENAIAKEADGDTYSLTTNILRGLNLGGFAQGLIPISNVTGEGLVNLQGALSRILNLGEEVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTZ0</accession>
    <name>A0A2S2KTZ0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Aspartokinase</fullName>
            <ecNumber evidence="13">2.7.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">NZNM25_17260</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>L-aspartate + ATP = 4-phospho-L-aspartate + ADP</text>
            <dbReference type="Rhea" id="RHEA:23776"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 14">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the aspartokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.4" evidence="13"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877539.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTZ0"/>
    <dbReference type="GeneID" id="76208259"/>
    <dbReference type="OrthoDB" id="8904at2157"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00015"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00461"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00462"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004072">
        <property type="term" value="F:aspartate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009090">
        <property type="term" value="P:homoserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04924">
        <property type="entry name" value="ACT_AK-Arch_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04921">
        <property type="entry name" value="ACT_AKi-HSDH-ThrA-like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2130.10:FF:000013">
        <property type="entry name" value="Aspartokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000017">
        <property type="entry name" value="Bifunctional aspartokinase/homoserine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000072">
        <property type="entry name" value="Probable aspartokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2130.10">
        <property type="entry name" value="VC0802-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054352">
        <property type="entry name" value="ACT_Aspartokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005260">
        <property type="entry name" value="Asp_kin_monofn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001341">
        <property type="entry name" value="Asp_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018042">
        <property type="entry name" value="Aspartate_kinase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00656">
        <property type="entry name" value="asp_kin_monofn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00657">
        <property type="entry name" value="asp_kinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004938">
        <property type="entry name" value="PRK06291.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21499">
        <property type="entry name" value="ASPARTATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21499:SF59">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22468">
        <property type="entry name" value="ACT_9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000726">
        <property type="entry name" value="Asp_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00324">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 13 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0791">Threonine biosynthesis</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="15">
        <location>
            <begin position="326"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GBH34935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="467" mass="50448" checksum="5C4B93D4345A0315" modified="2018-07-18" version="1">MRLVIKYGGTSISASKDIQAIAKHINSLSKKHQIVVVCSATSGTTDDLIEISESIKKENKSKAEQLASKITNRHKQLAKQTIKKPDLQKKLLRKLDEDFTELLSLIDGMVLLGEVTSRSMDYLISFGERLSIKLVSSAINDSGKKSIPLTGKEVGIITDSNFGESKPLMDTTRLRVSKTIDDLFSKKTIPVVGGFAGADQHGHVTTFGRGGSDYSATTIGSCIKADEIWLMSDVDGLMTADPKIVKNAKLLKEVSYIEAIEMAMFGAKQIHPRTFEPLLSKKIPMKIRNSFNTKNEGTLVTVSPSESSKNTVKCVSNVQNNGLIDIRGGSMVGTPGTAAKIFETLAKSGINVMMISQNPSESSITIVVKNTDLDKAVSALEMELLGKIIKKLEITTNVAIIALIGSGMRGTVGVASRVFGAIEKNKINISMITQGSSELNLAFVVKNSDTNTAVRALHDAFELDKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU02</accession>
    <name>A0A2S2KU02_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cytochrome oxidase subunit II copper A binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_19130</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome c oxidase subunit 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877727.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU02"/>
    <dbReference type="GeneID" id="76210142"/>
    <dbReference type="OrthoDB" id="3372at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045187">
        <property type="entry name" value="CcO_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002429">
        <property type="entry name" value="CcO_II-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001505">
        <property type="entry name" value="Copper_CuA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22888:SF9">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22888">
        <property type="entry name" value="CYTOCHROME C OXIDASE, SUBUNIT II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00116">
        <property type="entry name" value="COX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00078">
        <property type="entry name" value="COX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50857">
        <property type="entry name" value="COX2_CUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0186">Copper</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytochrome oxidase subunit II copper A binding" evidence="8">
        <location>
            <begin position="39"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH35122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="143" mass="16413" checksum="F9C818995F9CD799" modified="2018-07-18" version="1">MGGHSNWPEWVYIGVVTALMVWVGAEAWEAERLVEHVPADAEIIKVTGQQWFWSFEHEDGTKEIGELHVEKGKAYKFEIESKDVNHSFNIHDYVVLMDAIPGRVNTVWFAPTDVGEHDIQCREYCGLIHYNMRGKLIVEEPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU12</accession>
    <name>A0A2S2KU12_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome oxidase assembly protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">NZNM25_19100</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="11">Porphyrin-containing compound metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877725.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU12"/>
    <dbReference type="GeneID" id="76210139"/>
    <dbReference type="OrthoDB" id="336837at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003780">
        <property type="entry name" value="COX15/CtaA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050450">
        <property type="entry name" value="COX15/CtaA_HemeA_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35457">
        <property type="entry name" value="HEME A SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35457:SF1">
        <property type="entry name" value="HEME A SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02628">
        <property type="entry name" value="COX15-CtaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="111"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH35119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="147" mass="15843" checksum="8465ABB9A51B4E4E" modified="2018-07-18" version="1">MAIQYLALTTLVVLYSLMFIGGYISAAGLGLTCPEWPLCPNGVMPSEEYLIEWIHRTTAATTGLLVISTMIASLINKNSNLKIKITSSLATGLVITQITLGALVIDTKLHAVLVAIHLGIGIWLFAMVLLTTLFAFRISRITVKPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU45</accession>
    <name>A0A2S2KU45_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TrmB family transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_19810</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transcriptional regulator TrmB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877789.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU45"/>
    <dbReference type="GeneID" id="76210211"/>
    <dbReference type="OrthoDB" id="30795at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09124">
        <property type="entry name" value="PLDc_like_TrmB_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051797">
        <property type="entry name" value="TrmB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021586">
        <property type="entry name" value="Tscrpt_reg_TrmB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11495">
        <property type="entry name" value="Regulator_TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="2">
        <location>
            <begin position="11"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription regulator TrmB C-terminal" evidence="3">
        <location>
            <begin position="118"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH35190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="255" mass="27932" checksum="95664A28B483B492" modified="2018-07-18" version="1">MSISDKTRKALEKIGLTSYEIRTFSALLKAGELTASDLSQKSGVPYSKIYEVLGTLEDKGWIGSDDSRPTKYFAKSPSTGLETTKQKMETDFSQNQSVILNELVPLYEKSGTSERPDIWVLSGAINIAAKILEMVDTCRNEVMIALPEAGVELVKQALPKLRSLHDKGVEITILTSDKMDKESIKAIKRVATVKIKKGLFGGGIISDKRYVVILLGPEIGGANTSDVVAIWADHAGLAGFARQYFEYLLKDSKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU57</accession>
    <name>A0A2S2KU57_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acylneuraminate cytidylyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">neuA</name>
        <name evidence="1" type="ORF">NZNM25_19740</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU57"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008781">
        <property type="term" value="F:N-acylneuraminate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02513">
        <property type="entry name" value="CMP-NeuAc_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050793">
        <property type="entry name" value="CMP-NeuNAc_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003329">
        <property type="entry name" value="Cytidylyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21485">
        <property type="entry name" value="HAD SUPERFAMILY MEMBERS CMAS AND KDSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21485:SF6">
        <property type="entry name" value="N-ACYLNEURAMINATE CYTIDYLYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02348">
        <property type="entry name" value="CTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="28842" checksum="FF2E0FA6A00DCB72" modified="2018-07-18" version="1">MISLTSNLFNTQKFSEIEILKTICFIGARGGSKGVPRKNIRLLNKKPLIAYAIESALESRLFNHVIVSTEDPEIAKISKKYGAEVPFKRPKNLASDSTGFADVMLHGIKQLRQLDYQFDVLVNRDCTVPFIRISDMKKAIDLLKNKKPNAVYGVYRQHFNPYFNMMEKDLSGFLRLCKKLKNRPKSRQEAPIVYQLNGLFVYDVAQFLKYKNPILPKSLPLEIPLECGHMIDTEIEFRLAEMMIKQKLIPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUB7</accession>
    <name>A0A2S2KUB7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-synthesizing GTPase</fullName>
            <ecNumber evidence="2">3.6.5.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_20230</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EIF2G subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877838.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUB7"/>
    <dbReference type="GeneID" id="76210257"/>
    <dbReference type="OrthoDB" id="7798at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01888">
        <property type="entry name" value="eIF2_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03688">
        <property type="entry name" value="eIF2_gamma_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15490">
        <property type="entry name" value="eIF2_gamma_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000009">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000065">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000075">
        <property type="entry name" value="Translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050543">
        <property type="entry name" value="eIF2G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015256">
        <property type="entry name" value="eIF2g_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044127">
        <property type="entry name" value="eIF2g_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044128">
        <property type="entry name" value="eIF2g_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022424">
        <property type="entry name" value="TIF2_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03680">
        <property type="entry name" value="eif2g_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003077">
        <property type="entry name" value="PRK04000.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09173">
        <property type="entry name" value="eIF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 12" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="18"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH35232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="420" mass="45474" checksum="757C33DFB28F2CC8" modified="2018-07-18" version="1">MHWRETLPDWYIKKYGYQPCVNIGTAGHVDHGKTTLIQALTGSWTSVHSQELKRGITIRVGYSDAAFYKCKSCEEPLGFSTTPKCNNCGKESELSRVVSFVDSPGHESLMANMLSGSALMDGALLLVAANEKVPKPQTKEHLLALQTLGIQQIVVVQNKVDLLPYQEVMANYQDITKFVKGTFAAKAPIIPISAQSGLNIDALIGSIESTIKTPERDEKKDTVMHVLRSFDVNKPGIKLKDIKGGVIGGSLTQGVFKIGDEIEIKPGIMNEKKKTYEPLLTEITSLGTAAGIVESVKPGGLVAIGTKLDPSMTRSDSFIGSVIGKPGTLPENSTELKLEVNLFDVAVGITEDTKVQPIQAGELLRLNIGTAPVLGKVTKIKSKNIEIELRRPACIFEGGNVAISRRIDERWRLIGAGIIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUI2</accession>
    <name>A0A2S2KUI2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RecA family profile 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_20080</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877893.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUI2"/>
    <dbReference type="GeneID" id="76210242"/>
    <dbReference type="OrthoDB" id="17644at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942:SF30">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN DMC1_LIM15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942">
        <property type="entry name" value="RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="4">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH35217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="219" mass="24943" checksum="50B54950D726B9D0" modified="2018-07-18" version="1">MISTGLQKLDEFLSGGISGGIIVDIFGGNGTGKTQFLLQLCMNSIKNGGNVLYLDTTGGFRPERILEIQKQSEIEFDFLDKITVSRIRNTSEQIKSINNFKKNHFSLIVIDNITDLFSYEYKNEESIFEKNSLFMKYMNQLSKFAINNKIPIVITNMIRILEGQEVENMKSAIDPFTHIKIHLSKKSSKFEGQIYSAFDKQYFSYSINTLGLTNNSEDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNS0</accession>
    <name>A0A2S2KNS0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00430</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNS0"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="61" mass="7160" checksum="727970F692C5CC71" modified="2018-07-18" version="1">MFLKNLTKNKMHSKIVVNKSSNAIIYSSDFSMYLQEKTTKKAKKLKMFAKAIDPLWYVKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNU6</accession>
    <name>A0A2S2KNU6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribonuclease P protein component 1</fullName>
            <shortName evidence="6">RNase P component 1</shortName>
            <ecNumber evidence="6">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Rpp29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rnp1</name>
        <name evidence="7" type="ORF">NZNM25_00280</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eukaryotic/archaeal RNase P protein component 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875910.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNU6"/>
    <dbReference type="GeneID" id="76209354"/>
    <dbReference type="OrthoDB" id="39019at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.210">
        <property type="entry name" value="Ribonuclease P/MRP, subunit p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00754">
        <property type="entry name" value="RNase_P_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036980">
        <property type="entry name" value="RNase_P/MRP_Rpp29_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023538">
        <property type="entry name" value="RNP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023534">
        <property type="entry name" value="Rof/RNase_P-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002730">
        <property type="entry name" value="Rpp29/RNP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01868">
        <property type="entry name" value="RNase_P-MRP_p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00538">
        <property type="entry name" value="POP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101744">
        <property type="entry name" value="Rof/RNase P subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="88" mass="9860" checksum="3404D2948912B853" modified="2018-07-18" version="1">MITADNITSHEFIGLHTEIVKSTNPQVIGLNGRIINETKSMFTINTEKGIKSIAKSTNNWKFSIENKDVIVEGSRITKRPFDRIGDKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNX5</accession>
    <name>A0A2S2KNX5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01740</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876043.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNX5"/>
    <dbReference type="GeneID" id="76209506"/>
    <dbReference type="OrthoDB" id="2626at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="203" mass="23684" checksum="4A8C6682A2FA82A2" modified="2018-07-18" version="1">MKEISISSLLKNMNQENNYEDDLWKTCLDEKGRRYRRKDISNSLKKLAVLGFIKKIRISGTDTYYNKIYYSNSDDYVGFVNNIMFSNESKIKESLKKLESKKIFVDISKDLNSYKLSDQSKVNYEKLLEAFSNLTELASAIQLVKDTSTDEDLKKKLNTCHLEIKETLKETGEKIIRDRKSNEIIVLQRRFSGRIPNPGFLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNY7</accession>
    <name>A0A2S2KNY7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_01470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876016.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNY7"/>
    <dbReference type="GeneID" id="76209477"/>
    <dbReference type="OrthoDB" id="386731at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="351" mass="41330" checksum="B6B5B3F506517E02" modified="2018-07-18" version="1">MARREVLMTGLSLFLAIVVGILMDWDFKKIDSNESLISYFLIGGIVTSIIYGFKPRAEKYFEKDESKTRRIEKIITETSADKRKDLDEYSGHLKKDFLSLIESEMENTNTISYYDAIKNLKTHKKMLLQHLYTFEKGVFISPPIYTRYFEARETDGKLEHIWSELSNMSTRFYTIAIKEFKSKEFWTIDTEKLLGMIGIPKVTHLTKNVNLDNIHPKSPTEIYKGSFTYEEDQNSYTKKYFNIKYNGEIVSKSESKKIAKKLVKMLHQQCIYSSKKMLNILDMKKENEKFIKPAVHTMFKEIAESVRVGKPKLGVCDACIGFYPFERKMHYQQYLDEFNSTPWSWSDDHWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNZ8</accession>
    <name>A0A2S2KNZ8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_00950</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829018.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNZ8"/>
    <dbReference type="GeneID" id="76209423"/>
    <dbReference type="OrthoDB" id="6452at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="13"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="57" mass="6755" checksum="B0A2AD3F0B656AD9" modified="2018-07-18" version="1">MVLPLVDEHKPKCYLCHEGFENIEKLREHQKLKHKEFSESHENNIKRQPAPGDVTVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP11</accession>
    <name>A0A2S2KP11_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875900.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP11"/>
    <dbReference type="GeneID" id="76209342"/>
    <dbReference type="OrthoDB" id="8448at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="113" mass="13256" checksum="3BA0844412C34F54" modified="2018-07-18" version="1">MIKTTIELPISDNLSDAELKKIKEYFKEMPVKEILTGLKFAKNRWSAKDAGTLKVGRKSIIQKEVHSVTAEQAQWRLKNWKMMIANYRRRGYSYPTISRIKKILIQKSKKKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP16</accession>
    <name>A0A2S2KP16_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fido domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_02110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876079.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP16"/>
    <dbReference type="GeneID" id="76209545"/>
    <dbReference type="OrthoDB" id="123270at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1870">
        <property type="entry name" value="Fic/DOC protein, Fido domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003812">
        <property type="entry name" value="Fido"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036597">
        <property type="entry name" value="Fido-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053737">
        <property type="entry name" value="Type_II_TA_Toxin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02661">
        <property type="entry name" value="Fic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140931">
        <property type="entry name" value="Fic-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51459">
        <property type="entry name" value="FIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fido" evidence="1">
        <location>
            <begin position="7"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="140" mass="16161" checksum="0153504E0DD5E808" modified="2018-07-18" version="1">MVNYEIFSPDAIKNISLINKHVVEKRPESFGVDDPSIITKIFERVNQYFHIPDKKIRVIKRASHLLAGIVFEQPFKNGNKSTAFIVTQHYLKINGFVFSVDISDDELIQLLESVSPLFLNEDQYNIAFSTVEEFLIRITN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP32</accession>
    <name>A0A2S2KP32_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01350</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876006.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP32"/>
    <dbReference type="GeneID" id="76209464"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="206" mass="22805" checksum="BE68D44A2E541591" modified="2018-07-18" version="1">MINKKISLMAVIAIAAFASIVYGVSENSPVIERTGYTQFADRTFDQRVEINALAVVGEIVDSEIVMVEETVTGTDCDECEEYVIETAVKPQYKVTVKVTEVLKDNGILNGNDLVTFYDKSVTGLGKVDGHTTQFISRYSVDYHVGDKGIFIISNDRGLNMMGFTHYYPLKAGQTTMTSEFDKMTEKAPIDVDSARNIAKMIAEKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP36</accession>
    <name>A0A2S2KP36_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fibronectin type III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_02070</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866810.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP36"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="11341" checksum="04D2F03155158EFD" modified="2018-07-18" version="1">MCVAVSGLFGTAYAQPLDDINATVLEYDDSLASVHLTWNHDDSISLYEIGCVSCIPNFSENTTYDEIVLSNITSLGNGIAILYVIAYHDDSDMITAKQVMLELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP42</accession>
    <name>A0A2S2KP42_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875959.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP42"/>
    <dbReference type="GeneID" id="76209411"/>
    <dbReference type="OrthoDB" id="4385at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="75" mass="8839" checksum="9928BB97585B3527" modified="2018-07-18" version="1">MGLLYTKFYLDFAENEWKQISNNPIVFQTIKDGVSIDIEDDSHKSYKLNFKEGGKLNMFRVTGKFRLTWNDDDVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP64</accession>
    <name>A0A2S2KP64_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator AsnC/Lrp ligand binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_00490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP64"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="1">
        <location>
            <begin position="9"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="84" mass="9526" checksum="4F5194B36AF1BB44" modified="2018-07-18" version="1">MNKLETALVLVKSEISHEMDVMRQLLKIDQVKEAKGTFGLYDIFVKIEANSSLEIADIITKQIRKITHVISTTTLSVIPEQNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP88</accession>
    <name>A0A2S2KP88_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Proteasome assembly chaperone family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_02400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876102.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP88"/>
    <dbReference type="GeneID" id="76209574"/>
    <dbReference type="OrthoDB" id="35908at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610:SF3">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159659">
        <property type="entry name" value="Cgl1923-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="207"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="26062" checksum="AE2B04B09E597D01" modified="2018-07-18" version="1">MLPEIKIREIIPINLEGGYLIDGFPSVGFSSAIASESMINTSNFELAGTIDSESFPPISIIKNKIPNFPTRIFVNESLKVGIFLSYFTLEESLHRVTANAMLEWAKKHKIELIVSSVAIKNPKGNEEMIGIGSTESAREKIKDAGLKVLEHGTVPGIPGILLNEGSMKKQDVIVIIFHTDGQGPDFKSSANLCLAMSKLIPGASCNIPSLQKEAEKAEEMIKEAQEESKHLKDYMYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP99</accession>
    <name>A0A2S2KP99_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_02500</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876113.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP99"/>
    <dbReference type="GeneID" id="76209584"/>
    <dbReference type="OrthoDB" id="6846at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="128" mass="14857" checksum="12A54ABE2DAEAA14" modified="2018-07-18" version="1">MRDSETFGVEKGHGKEIVSWLNNQAKIQGVKLEARLYGYDISTKNFGDFELFSWIGDVQIARKMIIKASKRFKVKVIEGGYKPKEKLLRLKKFDFAKVKKGDKTIGLLEFASSRFGNNQWEIQNEERH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPA2</accession>
    <name>A0A2S2KPA2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Protein translation factor SUI1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_02630</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the SUI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876122.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPA2"/>
    <dbReference type="GeneID" id="76209598"/>
    <dbReference type="OrthoDB" id="11182at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11567">
        <property type="entry name" value="YciH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.780.10:FF:000017">
        <property type="entry name" value="Protein translation factor SUI1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00604">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050318">
        <property type="entry name" value="DENR/SUI1_TIF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022851">
        <property type="entry name" value="SUI1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005872">
        <property type="entry name" value="SUI1_arc_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002096">
        <property type="entry name" value="PRK00939.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789:SF0">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037511">
        <property type="entry name" value="Transl_init_SUI1_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 5" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="SUI1" evidence="6">
        <location>
            <begin position="27"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR037511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="102" mass="11258" checksum="30D08B2931AAD632" modified="2018-07-18" version="1">MAVICNTCGLPEDLCACGELAKDSTKIIIRLETRRFKKKGTMIEGLDPKLNNLETVAKELKNKYACGGTAKEGYIFLQGDHRDTIKDTLINLGFAADTIELH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPA5</accession>
    <name>A0A2S2KPA5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_02770</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPA5"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="60" mass="6885" checksum="6CD5D33231EF0018" modified="2018-07-18" version="1">MMFSKKKITLDYTVEKCSNCGMQRKRKFSDGDVLFAECSKCDSCGEMCLIEKIFGETLEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPA7</accession>
    <name>A0A2S2KPA7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_02150</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPA7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="440" mass="50848" checksum="9AE2DB3CEF0CB4AD" modified="2018-07-18" version="1">MISYGMKYFVIFLIFVGFVGLAHAQSDDYSPSGRDYVPYTQNYNVRFTSDGYIDYDLLIAKIMPEIFQKKLRIEYDVYISTQDIVLERGPQIAMYTPNSYNCGYVIDYTDNQVYWLESAINSTDIQYTKIFTETPTPKSVHGLEIEESNFGWCFGPLKQQVAAIFLEEKSYLTDDQEAIVSAAVKHELRGNPNLNDQEFTVGKFNFDYGENVLSFCGEFQKDRHGLKYFGGSMRNNILGDFHLDDKLSPLCAISDNAKTHSIKVNKKSDVPPELQTWKNLRMETVFLKPNSVEKLLERNYMYPETMHYNLFEYSTPFKINLIEFMPEDSNTIWNFEPSTDDTFVKIRVPKNGGNYYMSYGPNEWNAGNLTGVIILVNGVEVNYNTQLFIRAPDAPYPQHHTDYVFKVPKGSSSIELILFIENYVPENVDDDEYQPYTPQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPB5</accession>
    <name>A0A2S2KPB5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_02730</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_264953652.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPB5"/>
    <dbReference type="GeneID" id="76209610"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06225">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00075">
        <property type="entry name" value="HATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024478">
        <property type="entry name" value="HlyB_4HB_MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065:SF10">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065">
        <property type="entry name" value="SENSOR HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12729">
        <property type="entry name" value="4HB_MCP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158472">
        <property type="entry name" value="HAMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="11">
        <location>
            <begin position="216"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="10">
        <location>
            <begin position="284"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH33482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="507" mass="57479" checksum="6D0CF59C75487740" modified="2018-07-18" version="1">MVLEIIVLFALVGMISFVSIMNTKQLQDTFSSINSETLPILDTLKNMRYASTQLSAITMEIVLIEDETRNVQENEYRELEEILEINFYRIEQAKSLFNDAFSKYSNIMSKNYPDEINHTEELAEKWNEMLFVSNKMIQTKTSGISGITILNLNKDFDSSFDAMNAEIDRAITLTSGNIDQRQIYIDSLVSEVTWSIVVALNMFVLTALIIRFLILRSISNPLNKIRQVTHSIAKGDYVMYPVKGNDEISELGKDVNIMSKDLAELHKKIVEKERLSSIGSLATRFAHDIRNPLSVIKNSFEIIEIKTKNTLDDGMIKNFERIGRAVERITHQTDDVLDFVNVTELKREKQSLLSILKSTIKNIKIPYGVKIILPENDAVILCDPYKLEIIFTNLINNACYAINNDGQIAFRIVENDDDVLIEIEDSGKGIPEGEIDKIFEPLFTTKQTGTGLGLSSCQSIVEAHEGKISVRNNPTTFSILLPKNSRNHQIPIKKEYAKLKEGHIATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPE5</accession>
    <name>A0A2S2KPE5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PHP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_02280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPE5"/>
    <dbReference type="OrthoDB" id="50465at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07432">
        <property type="entry name" value="PHP_HisPPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052018">
        <property type="entry name" value="PHP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42924">
        <property type="entry name" value="EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42924:SF3">
        <property type="entry name" value="POLYMERASE_HISTIDINOL PHOSPHATASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13263">
        <property type="entry name" value="PHP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="304" mass="34710" checksum="A231A2BFD03B5ABA" modified="2018-07-18" version="1">MPINAELHCHNSFSNFHVGDKEPPYDCNISIRDQLERSYNLGLNAIFVTNHNTLDGYHQLLEYKNDHEKFKNIDVFPAEEITIDNGAHVLAYGLYDEIPVGLSLDDVIDKVRDQGGVSSAPHPFSLLDALRDNAKKCDMVEVFNSNNIDILSNARATQFALDNDMIQVAGSDSHVISTLGRCVNVIDSENNLDDILQAMKKGKISISQTGYALQSETLDHLKYKINNSKDYLIDYISEHYPNSKWLLTLLLRIYDSNQNSHMWSLFYNIAIYLMRRISQKINFQNHDPGFMKDRNLATMFKMAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPJ4</accession>
    <name>A0A2S2KPJ4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_02420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_263970046.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPJ4"/>
    <dbReference type="GeneID" id="76209576"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="43" mass="4973" checksum="97BF4505186144FD" modified="2018-07-18" version="1">MQILNEINTFEKSLDPEICEMIVEKINLFNDSCEPKIEILDCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPJ5</accession>
    <name>A0A2S2KPJ5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_02060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPJ5"/>
    <dbReference type="OrthoDB" id="3014at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="342"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="404"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="474" mass="55079" checksum="20B054632EDF534F" modified="2018-07-18" version="1">MFGVAILGICVVFAFLASPIYAQEPVETSNQTTLSGDLSNNPVAQDILKKIEQTKKWIRDLEQKDYEKLEAQKELEAKRAQSLQILNQDLADWEKLWDYYSSYNAFDRFIDKIPDSQVQDVFWDQFEFKEQKVEAGRDAFKQVIADGGSRRDALQAYLAAAETKRIELIETNSQFNVNHNLAYYSQQILFDKEGQFIDSPITGEQLRKYYEDYRTNPAYLQANPNDVVSWEDLGKTTPDTECREGNVVVYRFHAEDYVCVSISTAEMWVRHGMGEITGNTQNRFSQEPITPLTKCNDGFSVIFNLETRKYSCVPDDTAKEWMEQGLAEFHNVDDYVATSIENKDKSVRVEEVNLQIREMKQELEEKQMELKKEYDKKYDDAISQSKIDEKNATVDYNQRPGMSKEELSIKIGKIRENLESEKESILEKKIDDLKNLEKEFKDRMKELVSLYDDDPYVEVRLNMGHTGYEAVSRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPJ6</accession>
    <name>A0A2S2KPJ6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_01790</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876047.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPJ6"/>
    <dbReference type="GeneID" id="76209510"/>
    <dbReference type="OrthoDB" id="372125at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051493">
        <property type="term" value="P:regulation of cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01896">
        <property type="entry name" value="DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01666">
        <property type="entry name" value="TGS_DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000003">
        <property type="entry name" value="Developmentally-regulated GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002634">
        <property type="entry name" value="Small GTP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045001">
        <property type="entry name" value="DRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031662">
        <property type="entry name" value="GTP-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006074">
        <property type="entry name" value="GTP1-OBG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43127">
        <property type="entry name" value="DEVELOPMENTALLY-REGULATED GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16897">
        <property type="entry name" value="MMR_HSR1_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00905">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="8">
        <location>
            <begin position="64"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="9">
        <location>
            <begin position="290"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH33388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="367" mass="40764" checksum="F495C4756E23AAC2" modified="2018-07-18" version="1">MGIPEKIKAIQDEMAKTQINKATEHHIGLLKAKIAKLKREQEADITKKSGMKQDGFDVRRSGDATVVFIGLPSVGKSTLLNKMTGAKSAVGAFQFTTLTVVPGMMEYRGAKIQVLDLPGIIKGASTGKGLGKRILSVARTADLVLLVLDVFQPFHEDVLINELGNIGIRLNQLPPNITIEKAAMGGIAIAQQVKLTKITEKHLKDILHLYGIVSARVVVREDLTSEQLSDHIAGNISYSKALTILNKIDLVDKEFLKDLKTKIKSDVIEVSANSDINVGLLKEKIYEKLKFIRIYMRPKGGETDFKEPLIAREGDTVEDICNKLHRRLKREFRYGLVWGKSVKFGGQRVGLNHILLDEDILTIIKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPM9</accession>
    <name>A0A2S2KPM9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876162.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPM9"/>
    <dbReference type="GeneID" id="76209645"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="61" mass="7124" checksum="07E5A1794B477547" modified="2018-07-18" version="1">MVITKISECLVCNTLKNNLTEHHVKECNGEIMMVCNDCHKVITWYQDQAIPKFKEQLEKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPT2</accession>
    <name>A0A2S2KPT2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Methionine aminopeptidase</fullName>
            <shortName evidence="8">MAP</shortName>
            <shortName evidence="8">MetAP</shortName>
            <ecNumber evidence="8 9">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">map</name>
        <name evidence="11" type="ORF">NZNM25_02760</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="8">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase M24A family. Methionine aminopeptidase archaeal type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="8 9"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPT2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004239">
        <property type="term" value="F:initiator methionyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01975">
        <property type="entry name" value="MetAP_2_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050247">
        <property type="entry name" value="Met_Aminopeptidase_Type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028595">
        <property type="entry name" value="MetAP_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002468">
        <property type="entry name" value="Pept_M24A_MAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018349">
        <property type="entry name" value="Pept_M24A_MAP2_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00501">
        <property type="entry name" value="met_pdase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777:SF2">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01202">
        <property type="entry name" value="MAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="10">
        <location>
            <begin position="10"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH33485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="302" mass="33571" checksum="732A9DF274B2C822" modified="2018-07-18" version="1">MSKNVQLEDYLKAGKIAAEVREKVRIKDWIGKSVYEICEEVEGEIKKRGAKCAFPVNASINEIAAHYTAEPNDPITIKDTDMVKIDLGAQINGYIADTAVTVCYDAQFDGLVQAAEEALGNAMSMIKAGVKASDIGRTIETTIKQRGYKPIANLSGHSLEQYTIHAGKSIPNIWSIGGFSLSENSAYACEPFVTTEQGGGFVRNGQIKNIFAINSRKKTKDAEADRLLDFIWKNFNMLPFALRWITKEWEEKQARELLNVLIKKKAVQAYPILIEVNEQRVAQAEHTFIPLEDGVTVTTRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPU0</accession>
    <name>A0A2S2KPU0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04910</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPU0"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="522" mass="58783" checksum="50E5885665D79CCA" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPX5</accession>
    <name>A0A2S2KPX5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Potassium transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_04450</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TrkH potassium transport family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876317.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPX5"/>
    <dbReference type="GeneID" id="76209795"/>
    <dbReference type="OrthoDB" id="111943at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32024">
        <property type="entry name" value="TRK SYSTEM POTASSIUM UPTAKE PROTEIN TRKG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32024:SF2">
        <property type="entry name" value="TRK SYSTEM POTASSIUM UPTAKE PROTEIN TRKG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="281"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="383"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="416"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="470"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="519"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="578"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="11">
        <location>
            <begin position="21"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="11">
        <location>
            <begin position="87"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH33654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="604" mass="66700" checksum="ADE6DAFAFF4D2F36" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ46</accession>
    <name>A0A2S2KQ46_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA pseudouridine(55) synthase</fullName>
            <ecNumber evidence="1">5.4.99.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_03660</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="1"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876212.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ46"/>
    <dbReference type="OrthoDB" id="10348at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160148">
        <property type="term" value="F:tRNA pseudouridine(55) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039894">
        <property type="entry name" value="Pus10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048741">
        <property type="entry name" value="Pus10-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055174">
        <property type="entry name" value="Pus10_THUMP_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01213">
        <property type="entry name" value="pseudo_Pus10arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21568">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE PUS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21568:SF0">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE PUS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21238">
        <property type="entry name" value="Pus10_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22023">
        <property type="entry name" value="Pus10_THUMP_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pus10 THUMP" evidence="5">
        <location>
            <begin position="71"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Pus10-like C-terminal" evidence="4">
        <location>
            <begin position="160"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF21238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="380" mass="43401" checksum="546A5F9109BA4B79" modified="2018-07-18" version="1">MSDYQKIIPIANKILKKYSLCDNCLGRLFSKQLRLSSNKLLGKKLASNQNLSKCYICKNLFENLNYFLKLMLDASINYSYRTFGVGVMLKPSIVDRDDSIRSQYKLKGIDSIKTDITRELGKSFSRKTKKTFDTQNPEIIFTVNLKDNSIQLRSKPITFSGRYVKTMRNIPQKQRSCENCSGKGCRMCDFHGISEFQSVEGFISKLLFKKIGCTTAKFTWIGGEDKSSLVLGTGRPFFVKLKNPVKRNLRLTSFSTDGLKISKIKLVNESPKNPLKFNSLIQLKISTASFLDPKNLQKLKDLRKHPVVVYDKSGKRSEKQIFFVKYKKNSKNTFTLIMKTEGGLPVRRFIDGDDVSPGISQILNTSCKCHEFDFIDIEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ51</accession>
    <name>A0A2S2KQ51_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit Rpo3</fullName>
            <ecNumber evidence="4">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpo3</name>
        <name evidence="4" type="synonym">rpoD</name>
        <name evidence="6" type="ORF">NZNM25_04680</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="4"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829036.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ51"/>
    <dbReference type="GeneID" id="76209820"/>
    <dbReference type="OrthoDB" id="84933at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="entry name" value="DNA-directed RNA polymerase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00320">
        <property type="entry name" value="RNApol_arch_Rpo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001514">
        <property type="entry name" value="DNA-dir_RNA_pol_30-40kDasu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022842">
        <property type="entry name" value="RNAP_Rpo3/Rpb3/RPAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050518">
        <property type="entry name" value="Rpo3/RPB3_RNA_Pol_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001988">
        <property type="entry name" value="PRK00783.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800:SF2">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00446">
        <property type="entry name" value="RNA_POL_D_30KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 4" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RpoA/D/Rpb3-type" evidence="5">
        <location>
            <begin position="22"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="221" mass="24269" checksum="AB500CDF760C484D" modified="2018-07-18" version="1">MDFEDFVQDLSSLEVISKDNHKIAIKLKGIPLQYANALRRVCLNGVPVFAIDTVDIIENSSVLPDEGLAHRLGLIPLKTDLNRFNEPSKCDCKSETGCSNCRVMLVLDSGDSDVTRTILSNELSSEDETVKAVSDKIPIVQLAPGQRIKIECYARLGRGTEHAKWNSANISTLIDTDKENEKVLTVESTGALDPEQIVLAGVEEVSNKLGQFKETIGQIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ71</accession>
    <name>A0A2S2KQ71_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein uS13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rps13</name>
        <name evidence="7" type="ORF">NZNM25_05900</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 5">Part of the 30S ribosomal subunit. Forms a loose heterodimer with protein S19. Forms two bridges to the 50S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876441.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ71"/>
    <dbReference type="GeneID" id="76209942"/>
    <dbReference type="OrthoDB" id="372127at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.50:FF:000001">
        <property type="entry name" value="30S ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.910.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="entry name" value="30s ribosomal protein s13, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01315">
        <property type="entry name" value="Ribosomal_uS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="Rbsml_uS13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_uS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018269">
        <property type="entry name" value="Ribosomal_uS13_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003140">
        <property type="entry name" value="PRK04053.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S13/40S RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00646">
        <property type="entry name" value="RIBOSOMAL_S13_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="131"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="148"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00063089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="181" mass="19417" checksum="5D1F7E592B8319BA" modified="2018-07-18" version="1">MSTQEYRHIVRIVGNDIPGERKMLVGLTQIKGIGYNFATAILDTLKINTNSNIGNLTEENVQAIEKLITDPVGGNFPTWFLNRRKDIETGANLHLLTSDIPFTLRNDIERERITASWRGYRHLSGLKVRGQRTRTSGRKGGAVGVAKGGMAAPAKKGSAGAPAAVAAPTAEAAAPAPEKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ75</accession>
    <name>A0A2S2KQ75_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03960</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876278.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ75"/>
    <dbReference type="GeneID" id="76209742"/>
    <dbReference type="OrthoDB" id="6947at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="103" mass="12026" checksum="547D419427C503B2" modified="2018-07-18" version="1">MDFHPKDLPISKTYDLKDEKNAMEAVEDMIRIGFKKRKEGFKVLMPKESKIAKRIGYTVTTGVTHGLRQKNEIRDIRYWTYHHDDEHYAIVLISNSALADLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQB9</accession>
    <name>A0A2S2KQB9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HEAT repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_06710</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876509.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQB9"/>
    <dbReference type="GeneID" id="76210030"/>
    <dbReference type="OrthoDB" id="12160at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026003">
        <property type="entry name" value="Cohesin_HEAT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12765">
        <property type="entry name" value="Cohesin_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="177" mass="20010" checksum="8667F031767CAEDB" modified="2018-07-18" version="1">MENISKVLESGNSQEKIKILETLDKTDNPTILEKIISKLDDEDIQVRGEAFGSLVLNKNKISNFLIKSLNSTSKNIRGFALLVLANRNEIEAIPEIVKRVKDESSMVRSCAIGALRHLKAKEDKEIFLQSLLDSNLEVRKSALQAIIDLKISIPEDKRNEINKLKDLEMEKLISKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQC9</accession>
    <name>A0A2S2KQC9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mannose-6-phosphate isomerase type II C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_05480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876407.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQC9"/>
    <dbReference type="GeneID" id="76209899"/>
    <dbReference type="OrthoDB" id="5825at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004475">
        <property type="term" value="F:mannose-1-phosphate guanylyltransferase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005976">
        <property type="term" value="P:polysaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02213">
        <property type="entry name" value="cupin_PMI_typeII_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001538">
        <property type="entry name" value="Man6P_isomerase-2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051161">
        <property type="entry name" value="Mannose-6P_isomerase_type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46390">
        <property type="entry name" value="MANNOSE-1-PHOSPHATE GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46390:SF1">
        <property type="entry name" value="MANNOSE-1-PHOSPHATE GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01050">
        <property type="entry name" value="MannoseP_isomer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mannose-6-phosphate isomerase type II C-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="115" mass="13495" checksum="0A12A794EB247651" modified="2018-07-18" version="1">MRHKISKVVKVNKPWGFFEKLTDNETTTVKIITILAGNRTSLQFHEYRSEKWYVLDGQGYAVLDERQKIRAGDTIHIPTKAIHRLEAITDMKILEISFGLFQESDIKRLEDDYVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQF1</accession>
    <name>A0A2S2KQF1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA/RNA-binding protein Alba</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">albA</name>
        <name evidence="7" type="ORF">NZNM25_06700</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds double-stranded DNA tightly but without sequence specificity. Involved in DNA compaction.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="5">Acetylated. Acetylation at Lys-13 decreases DNA-binding affinity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the histone-like Alba family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876508.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQF1"/>
    <dbReference type="GeneID" id="76210029"/>
    <dbReference type="OrthoDB" id="10360at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01122">
        <property type="entry name" value="AlbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013795">
        <property type="entry name" value="DNA/RNA-bd_Alba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028732">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2 5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="3 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="4 5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Alba-like" evidence="6">
        <location>
            <begin position="8"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine" evidence="5">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="95" mass="10291" checksum="9E33CD28C35307B8" modified="2018-07-18" version="1">MSNEARDTIFIGKKPLMAYVTSTLIQLANLPSVNIKARGLSIGRAVDVAQIIARKTENAGYSIGEIKIGSESLESQDGRTRNVSTIEIEVKRNTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQL2</accession>
    <name>A0A2S2KQL2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Peroxiredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_06380</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876483.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQL2"/>
    <dbReference type="GeneID" id="76209995"/>
    <dbReference type="OrthoDB" id="6924at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050455">
        <property type="entry name" value="Tpx_Peroxidase_subfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43110">
        <property type="entry name" value="THIOL PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43110:SF1">
        <property type="entry name" value="THIOL PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="3"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="3">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="154" mass="17294" checksum="D7A45C7B47AAD170" modified="2018-07-18" version="1">MSLNIGDIAPNFELPDTELKLRTLDDFKGGKIVLSFIVAASSPVCETELCNFRDSWKEISDLGAKVVAISNDGPFANKAFAEKNHFNFPLLGDYSSKTIRDYGILMPDLLHIKGYNAAKRSVFIVNEDGKIGYKWVSEDPLKEPNYEEIKNFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQL8</accession>
    <name>A0A2S2KQL8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_07710</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQL8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="3">
        <location>
            <begin position="121"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="333"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="378" mass="45075" checksum="672EB660B0E21114" modified="2018-07-18" version="1">MFLDSVKNKETVRRYRNLLYTFLKLIPNQIYHDVLGEVPPDREKETLAKYFVDVARKDSEIAANIIAAYIKEDKKRIESGEISPQTIPNHIKPIKVLLDSNRIAIHWKSLTKLYPRITRGSNDRAYTREEIQKMLEVSKDITDKVIILMYSSGGFRLEAWDYFTWKDLVFFKNNDGSYKGAALIVYRNDPEQYWTFITPESCKALEIYREKWKSEIGQYPKPEEPLIRTVKFPMIRRLNQKGVRKRVEKVVREIGLRPPLPPGKRRHEVKLDHGFRKFFNTNLRRAKVDFLDKEDMMGHKIGLERHYERYQEEDFERFPEYEKAIPFLTISDEERAKLENIKLRQEKSELEKKNAELEAIKVKLDELWADKQRMENNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQM9</accession>
    <name>A0A2S2KQM9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_05490</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876408.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQM9"/>
    <dbReference type="GeneID" id="76209900"/>
    <dbReference type="OrthoDB" id="8852at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="115" mass="13693" checksum="1DC46CA70618FBD8" modified="2018-07-18" version="1">MEYEKLCRDVLDIDKKIRFAAIYNSKIEQIAGGMRKGVENLTPESITKLSVEQSFMRWHTRLEMNEWIGMPKYALAEYDRIKRFTFHINHEKLLLISTELDISNDFLIEKVRKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQN5</accession>
    <name>A0A2S2KQN5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ParB-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_07640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876624.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQN5"/>
    <dbReference type="GeneID" id="76208912"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16387">
        <property type="entry name" value="ParB_N_Srx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1530.10">
        <property type="entry name" value="Conserved hypothetical protein from pyrococcus furiosus pfu- 392566-001, ParB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="ParB/Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB-like N-terminal" evidence="1">
        <location>
            <begin position="49"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="341" mass="39920" checksum="AE98524BCCF046B4" modified="2018-07-18" version="1">MVKKTSEDYFEKTIPIKDITLDLENPRYHEKLIQTGKKKWTDEMLQEIIEDEDISDILPSIKASGVREPIWIFEKGNGKYEVLEGSRRLVVLRGLVRDKVDPPNSNISYDKVKCHIYPKNTDPRFIDAQRVILQTGKKKWGPFNEASAIYKLVHTDRYTTEEVAKMMRSSVGSVNKELENYKHYVDFTKFIKKDKVQGIEDPRKYLYFQRASAYVRDRFFGTLKEKEKFFKLITPNKHGETRIRSVSLKGGLYDFNKFAQNEIILQKFLKNDRMTIEDALDEYHGTSINDLMPWIKKLKDVAKGINNIDDDDLEKLKKETAIMNQIKRVYLGSKSIIESTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQN9</accession>
    <name>A0A2S2KQN9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07910</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876646.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQN9"/>
    <dbReference type="GeneID" id="76208885"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="195" mass="22632" checksum="A7D7B3F7E14C61C9" modified="2018-07-18" version="1">MKTRLLTVIIVMVVGSSVMVFADVVLQLKYQINNDLNPDNYVCWNSEHLLVERPNGKFACITPYMMIKLNWQPYKHEYYIFDVLKDNSVYAVFALDSNAVVSEIRYDVDLHSLIVDMPRNNSGKLFIEIPRDLLDAKLVRCDEVSEDPSDWDFFVLVDGEEVAFEEVFTTDDYRALEILYKKNSKQIEIVDACLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQQ1</accession>
    <name>A0A2S2KQQ1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 8">Isoleucine--tRNA ligase</fullName>
            <ecNumber evidence="1 8">6.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_07770</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 14">
            <text>tRNA(Ile) + L-isoleucine + ATP = L-isoleucyl-tRNA(Ile) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:9666"/>
            <dbReference type="Rhea" id="RHEA-COMP:9695"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="1 8"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQQ1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023586">
        <property type="entry name" value="Ile-tRNA-ligase_type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780:SF1">
        <property type="entry name" value="ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780">
        <property type="entry name" value="SOLEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19302">
        <property type="entry name" value="DUF5915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 9 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="10">
        <location>
            <begin position="35"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="11">
        <location>
            <begin position="697"/>
            <end position="845"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="NCBIfam" id="TIGR00392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH33986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="1073" mass="124301" checksum="08BC1C930CC4AF13" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR64</accession>
    <name>A0A2S2KR64_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876733.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR64"/>
    <dbReference type="GeneID" id="76208774"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="177" mass="19762" checksum="3F5AF073FABAEE40" modified="2018-07-18" version="1">MKNKIILHEKILILIISVIMASHIVVQVIVPTINSQVAEIEPIPMVTLTNETLHNAKNPNNGPQPQINSVERTMVVPERPTVLMMLGATLNHLAFALFVAFAGIGIALPWIGIIMLQFPSGPNAIPLFFFWFLSMASIPLVWWFSKSIHLLRRMLYLYMIPVLVAGIGITYFTNFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR82</accession>
    <name>A0A2S2KR82_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_07560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876616.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR82"/>
    <dbReference type="GeneID" id="76208920"/>
    <dbReference type="OrthoDB" id="377321at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="1">
        <location>
            <begin position="38"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="233" mass="27052" checksum="70E967B261C83948" modified="2018-07-18" version="1">MLTGNWPINYTFEDRYELTGITDFVLDYANNSKKKLIILDVGCSSGIAMKYVQDYLKQKNIETFTIGIDISKNVVKDASKNLDKFINEDLLNVNDFVKKADVVICSKAAIFVTGEIRYKIIRKCSDFLKNDGILITDVDCFEKSKLSDDLRDFLCEFPTWSCFKNGIGKFSKEFRRRQNIPYKKKMKKMSKDEGSKYADEILSAWNSFSFFKKLDWKISIIHRRFASSIVHGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR94</accession>
    <name>A0A2S2KR94_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH HARE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_07590</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876619.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR94"/>
    <dbReference type="GeneID" id="76208917"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1250">
        <property type="entry name" value="RNA polymerase, subunit delta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007759">
        <property type="entry name" value="Asxl_HARE-HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038087">
        <property type="entry name" value="RNAP_delta_N_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05066">
        <property type="entry name" value="HARE-HTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51913">
        <property type="entry name" value="HTH_HARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="HTH HARE-type" evidence="2">
        <location>
            <begin position="19"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="82" mass="9308" checksum="4E2A7139D7AABE1B" modified="2018-07-18" version="1">MKKSKNLKIVGVRMSDANDSIHGIVVRILKQSGKPMRVKDITEKVLKIKKIHSKTPMNSVVNKLQTSKHVIRIGHGLYSYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRA4</accession>
    <name>A0A2S2KRA4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_10010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRA4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050595">
        <property type="entry name" value="Bact_response_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591:SF3">
        <property type="entry name" value="RESPONSE REGULATORY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591">
        <property type="entry name" value="STRESS RESPONSE REGULATOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="9"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="124" mass="14069" checksum="D7469AE816BE5173" modified="2018-07-18" version="1">MTYFMSKKTILLVDDDIDLLENTAYMIKSMGFDVVTAEDGQDAVLKYKDINPDLTIMDVKMPKMDGFDAFFKIKQFDSEAKVVLITAFAVDEKKHLKAKSMSLVTTLNKPYSFEQLEEVVTAHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRE1</accession>
    <name>A0A2S2KRE1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_10410</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876859.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRE1"/>
    <dbReference type="GeneID" id="76208623"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="418" mass="47948" checksum="C46CB2862C9B7B5C" modified="2018-07-18" version="1">MKTRFLMFIGMIVISAGFLNTVQGFSDDGFDTGRFSDFHYGEKFYQYQYQPFGALVGDSSSRCHFTSDFILSENSLSESGHAKYKFPNDMIWPGGYESSTFFIINSPSFNFPADNNFEKIIPSQAKDGSVILEFDLVPGLNTFLANSTAFWDSQRSQMIDCPNPFDFEKQDAKYYDFVYPLKVQQNRAMTFDLDKNDFLCKSEFVLIQKYDDSPACVNKQTAVKLTNKEQRSGWNASEQLWKYLSSKMNELYSDNNCPKYCLILDEKYAVDNVGQTLGKTIGLPKFLPDAYEYFQFHHHETYSVIQISPVPISLNTSWNDFYWQDNGIFLRFSETPVTVDANASVEHWAKNHNAKKISLDSGELIYLKKRELGFNEQIGGIYPTLSEAQFVRDGLVISVAGYVSDENMENIVRSFYTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRJ0</accession>
    <name>A0A2S2KRJ0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chemotaxis methyl-accepting receptor HlyB-like 4HB MCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_10910</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876942.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRJ0"/>
    <dbReference type="GeneID" id="76208567"/>
    <dbReference type="OrthoDB" id="4905at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="18729" checksum="D9A484BDF90FB097" modified="2018-07-18" version="1">MASKKGIVITVIILAAITGASFLLWLVPPEGETTFVVSDYEGYLNGVKNIHEVLQESIEIEYQNLRNGEISPRDYIVVAEVTSSQVTSQISEFVTSKPPEIWQDSYISYMNAMKKFKSYIDETKVLANLIEKGSTDAEIDEVIEKIESLKKESLDYARNSEEQRPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRJ2</accession>
    <name>A0A2S2KRJ2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_10830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876936.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRJ2"/>
    <dbReference type="GeneID" id="76208576"/>
    <dbReference type="OrthoDB" id="10181at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="341" mass="36581" checksum="0D08C43157D0BD30" modified="2018-07-18" version="1">MKTSFLAIFVVAVLLTGTIAGPIGLIQSADALKSKGNSASAINSKKVCGDRLCSEPPKNEVKSSDKTKEEIKSEKKTESATKAQEAPKKDAKSETMEKAQEIATLKVPKTVTGVITSVQDPGQGHENHQLAILLPPSDKVYRGYVSFTATENVQLVALHGPLKAGMDKGQAIWTTDGKTKFGLTFVDKESSTGVWQFTGNAVALHTKNTEPFTVSYSITYTEQIVDGQKVFRSTMTSVQDPGIGHENHQLALLLAPRDKAYSGYLTYDASEPVQIVTLIGPLSQGELRGLPSWTPDGETFFALSIVPTKASGSVQFSGNAIALHTTNTEPFTVSYSLVLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRK0</accession>
    <name>A0A2S2KRK0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tritrans,polycis-undecaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific)</fullName>
            <ecNumber evidence="3">2.5.1.89</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Undecaprenyl diphosphate synthase</fullName>
            <shortName evidence="3">UDS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Undecaprenyl pyrophosphate synthase</fullName>
            <shortName evidence="3">UPP synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">uppS</name>
        <name evidence="4" type="ORF">NZNM25_10930</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with geranylgeranyl diphosphate (GGPP) to yield (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30E,34E,38E)-undecaprenyl diphosphate (tritrans,heptacis-UPP). It is probably the precursor of glycosyl carrier lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>geranylgeranyl diphosphate + 7 isopentenyl diphosphate = tri-trans,hepta-cis-undecaprenyl diphosphate + 7 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:27622"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:60388"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="2.5.1.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="3">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.89" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866873.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRK0"/>
    <dbReference type="GeneID" id="76208565"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016094">
        <property type="term" value="P:polyprenol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1180.10:FF:000005">
        <property type="entry name" value="Alkyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291:SF43">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT DHDDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="3">
        <location>
            <position position="34"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="35"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="79"/>
            <end position="81"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="212"/>
            <end position="214"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="257" mass="30298" checksum="69E171C05346D9E7" modified="2018-07-18" version="1">MVFQLSGLYKIYGRRLEREIQNGDIPNHVALILDGNRRWAKRHLTMPKKGHWKGADAVENLLDWCEEFDIKIVTLYALSAENLNRKDDELEYLYELIRMRLEKLYNDPRIHRCKMRVKGIGRIELLPDSIKEILGRLDEATKNYHNHFLNIALAYGGQYELVDAVKKIGEKIKDGSLNVEDINKKEIEANLYTSHLPQSSPDMILRTSGEKRLSGFLMWQSAYSELVFMDIFWPEFRKIDLMRAIRTFQERKRRLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRL1</accession>
    <name>A0A2S2KRL1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">L-threonylcarbamoyladenylate synthase</fullName>
            <ecNumber evidence="3">2.7.7.87</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">L-threonylcarbamoyladenylate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">NZNM25_11030</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonine + hydrogencarbonate + ATP = L-threonylcarbamoyladenylate + diphosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:36407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="EC" id="2.7.7.87"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SUA5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.87" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876952.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRL1"/>
    <dbReference type="GeneID" id="76208555"/>
    <dbReference type="OrthoDB" id="39992at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061710">
        <property type="term" value="F:L-threonylcarbamoyladenylate synthase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.10">
        <property type="entry name" value="DHBP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="Sua5-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050156">
        <property type="entry name" value="TC-AMP_synthase_SUA5"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00057">
        <property type="entry name" value="L-threonylcarbamoyladenylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490">
        <property type="entry name" value="SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490:SF16">
        <property type="entry name" value="THREONYLCARBAMOYL-AMP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="YrdC-like" evidence="12">
        <location>
            <begin position="7"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH34312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="198" mass="22214" checksum="5776F611BFE5A259" modified="2018-07-18" version="1">MKVKCDQDGIKRAVEIINRGGIIVYPTDTVYGIGCDPYNGEAVKKIYDIKSRNISKSLPVLTYSINTAKKIVFLDEFTEKIVEKFWPGPLTIVSKLIDEDLKKTLSLNDKIAIRVPKHDCILKILKECKFLVGTSANISGHPSFTNPDECFRSIENYDIFVDGGTITSKAESTIIEIENEKIKIIREGSLSKEDILEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRP6</accession>
    <name>A0A2S2KRP6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Abortive phage infection protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876959.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRP6"/>
    <dbReference type="GeneID" id="76208548"/>
    <dbReference type="OrthoDB" id="7920at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018891">
        <property type="entry name" value="AIPR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10592">
        <property type="entry name" value="AIPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Abortive phage infection protein C-terminal" evidence="1">
        <location>
            <begin position="227"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="583" mass="67214" checksum="78E1611190A0FAB8" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRR9</accession>
    <name>A0A2S2KRR9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Band 7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_10320</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRR9"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03401">
        <property type="entry name" value="SPFH_prohibitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000163">
        <property type="entry name" value="Prohibitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222">
        <property type="entry name" value="PROHIBITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222:SF0">
        <property type="entry name" value="PROHIBITIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00679">
        <property type="entry name" value="PROHIBITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="2">
        <location>
            <begin position="23"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="178"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="275" mass="30446" checksum="F5E53279ACC0C49F" modified="2018-07-18" version="1">MSAARGVAVGIILLIIIGVVISASVKIVDSGHRGVLLHWNAVDLTQPPLEEGLHFVVPFQDEVVNIEVRTLKYASDARSASRDLQTVETTVTVNYHPNREAVHRLYKDIGLDYENRVIQPAIEETVKQVTAKYNAEELITKRPLVKQDIEAAITERLNQFEVVTEVISITDFEFSPLFAQAIESKVEAEQNALRAENDLRRIEVEAKQREANAIGLANANIAEAKGEAEAIAIINRALAENPNYLDWLKTQAWDGKLPLVVGEGGTPFIQIPIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRT6</accession>
    <name>A0A2S2KRT6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876961.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRT6"/>
    <dbReference type="GeneID" id="76208543"/>
    <dbReference type="OrthoDB" id="3057at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="62" mass="6965" checksum="C5E1F41E850C8DBE" modified="2018-07-18" version="1">MVKILEMIGLVLVAIMSLGFVGVFEPYGIKNGTSELFWGCAGGTLLIIIYRKMKRKQDEKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRW3</accession>
    <name>A0A2S2KRW3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">NZNM25_11840</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877036.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRW3"/>
    <dbReference type="GeneID" id="76209322"/>
    <dbReference type="OrthoDB" id="9671at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000002">
        <property type="entry name" value="Magnesium/cobalt efflux protein CorC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005170">
        <property type="entry name" value="Transptr-assoc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22777">
        <property type="entry name" value="HEMOLYSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22777:SF17">
        <property type="entry name" value="UPF0053 PROTEIN SLL0260"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03471">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01091">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="10">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="9">
        <location>
            <begin position="205"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="9">
        <location>
            <begin position="270"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="417" mass="46461" checksum="A83C2F13F30DCDD9" modified="2018-07-18" version="1">MVELWMELSALAILIGLSGFFSGLEVALVGTRHSKVNQLRKQKVKGSEALYKLKHNPGWMMSSVNLGNNLVNVGSSAFATSVAIRIFGNDGLAIAVGIMTFLILVFGEITPKTYCNANATKIALRFAPVLLAFSYAFWPVVKLFEIITKTMVRITGSSYHAPPITEEEIKGIIEQGLADDALEKDESDLVHSALEFDDTVIRTVMTPRTKMTALPAKMLLFEALPLINQNAHSRIPIYGETNDDIIGFIHVRDVLKEVELDNKMKTLEQIAREPVFVSQEKMVSSLLKEMKGRKTHMAIVIDEHGGVEGLVTLEDLIEEIIGDIEDETDISPGVDHQSIDKDTIITSGEIEIERVNDIFKSDLPEGDDYSTLNGLLHERLQDIPQEGDKLELENLRIIVEKVSKNIPVKIRIERMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRX4</accession>
    <name>A0A2S2KRX4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">3-ketoacyl-CoA thiolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_11940</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877046.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRX4"/>
    <dbReference type="GeneID" id="76209312"/>
    <dbReference type="OrthoDB" id="167534at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00829">
        <property type="entry name" value="SCP-x_thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055140">
        <property type="entry name" value="Thiolase_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870:SF6">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22691">
        <property type="entry name" value="Thiolase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="2">
        <location>
            <begin position="4"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="3">
        <location>
            <begin position="226"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="369" mass="40346" checksum="9B417ED873B5249A" modified="2018-07-18" version="1">MKKVGIISYGITPFSKEDQKIESVLLKSVKNLFENNPNINRNDIDAVLVSTNNNSKYLSPILSEMIEIHPKIAHSIESLCNSGTNSIVSAYSYISAGLADMVLISGAERYDSPGQILEWDNSRGEYKHPIFWASIFTKSYKREFSISDEELAVVPVKNHKQAKENPNAMSNKNFSIQDVINSKKLTDDIRLLDCSRPCTGSASVILASEEIVRKNTDAPVWITGIGQKTTSASFTKNTSFSFMESTKLAVESALKMSNKTISDIDVAEIHDAFSVCEPMALESLGFANLGKGIDMIKELHETNNFKINPRGGLIGSGHPLGATGIAQTIEIIQQIQSNAMNRQVDNAKIGLVHNMSAAATSSTVLVLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS06</accession>
    <name>A0A2S2KS06_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877067.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS06"/>
    <dbReference type="GeneID" id="76209284"/>
    <dbReference type="OrthoDB" id="8516at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="115" mass="12549" checksum="D9F743870A14A58C" modified="2018-07-18" version="1">MQYFQALKLGQKRVAEARAYLNTLTDGRAMPALALASTDSNIWQPVGEENLYAFVDESAGFVLTDNSGYILALVDKTGSSKTIVQGVTPKQKENLEKVFKAANIPKFEGKVILPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS20</accession>
    <name>A0A2S2KS20_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_12710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877107.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS20"/>
    <dbReference type="GeneID" id="76209230"/>
    <dbReference type="OrthoDB" id="3122at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="141" mass="17292" checksum="C04C637094D3A4D9" modified="2018-07-18" version="1">MQAKISKELKDLERKLDKQRKEKVEQIIKEKLDQKKLDYDIITLILEIFDKSKFRWHKEHFDIFDSKSNSFRGKELPSNNRECVMLGLRLGTIRSKINYNLRDRQIKEEERQSIDDLVWDFVWYQWKEARMLYDYSTNGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS56</accession>
    <name>A0A2S2KS56_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase</fullName>
            <ecNumber evidence="10">6.3.4.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate--formate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 13" type="primary">purP</name>
        <name evidence="13" type="ORF">NZNM25_12350</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the ATP- and formate-dependent formylation of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (AICAR) to 5-formaminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (FAICAR) in the absence of folates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + formate + ATP = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24836"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (formate route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.23" evidence="10"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877082.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS56"/>
    <dbReference type="GeneID" id="76209269"/>
    <dbReference type="OrthoDB" id="98133at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00134"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01163">
        <property type="entry name" value="IMP_biosynth_PurP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023656">
        <property type="entry name" value="IMP_biosynth_PurP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009720">
        <property type="entry name" value="IMP_biosynth_PurP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010672">
        <property type="entry name" value="IMP_biosynth_PurP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38147:SF2">
        <property type="entry name" value="5-FORMAMINOIMIDAZOLE-4-CARBOXAMIDE-1-(BETA)-D-RIBOFURANOSYL 5'-MONOPHOSPHATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38147">
        <property type="entry name" value="5-FORMAMINOIMIDAZOLE-4-CARBOXAMIDE-1-(BETA)-D-RIBOFURANOSYL 5'-MONOPHOSPHATE SYNTHETASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06849">
        <property type="entry name" value="DUF1246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06973">
        <property type="entry name" value="DUF1297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004602">
        <property type="entry name" value="ATPgrasp_PurP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMP biosynthesis enzyme PurP N-terminal" evidence="11">
        <location>
            <begin position="4"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="IMP biosynthesis enzyme PurP C-terminal" evidence="12">
        <location>
            <begin position="156"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</name>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</name>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</name>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF06973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH34444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="341" mass="38563" checksum="A954EDA21FFD8A41" modified="2018-07-18" version="1">MVAIATLGSHCALQVLKGAKDEGIKSILICEKKREKLYRRFPFIDELIIVDSFKEVLDQKCQLILEENKAILVPHGTLIAQMNSEEIESIKTPVFGNKWILRWESDREMKEKLMVEAKLPVPKPVSNPKDINKLVIVKRQGAAGGKGYFMAANEEDYNIKKNQLIADGIISKEETLYIQEYAAGVLAYLQFFYSPLNEELEFFGVDQRHESDIEGISRIPSEQQLKSKKVPSFNVIGNSPLVLRESLLEEVYNMGESFVDASKKMVSPGMNGPFCIEGVYDENAKFTSFEFSARIVAGTNIYMDGSPYYSLLFNESMSMGRRIAREIKIATETNQLDKITT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS58</accession>
    <name>A0A2S2KS58_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pentapeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11880</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877040.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS58"/>
    <dbReference type="GeneID" id="76209318"/>
    <dbReference type="OrthoDB" id="4892at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.80">
        <property type="entry name" value="E3 ubiquitin-protein ligase SopA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051082">
        <property type="entry name" value="Pentapeptide-BTB/POZ_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14136">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN KCTD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14136:SF17">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN KCTD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141571">
        <property type="entry name" value="Pentapeptide repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="164" mass="18431" checksum="121F879705624AB4" modified="2018-07-18" version="1">MDSDVFRKRNLTKINFDYEELIGRNLANTNMSGVNLKNRDIHDADLSCTDLRGANLSYSILFYVKLRGADMRNADLSNAKLWDTEMYGVDLRGADINGADLFYADLRNADLSDANLSGINLRHTNFEGAILTSADFTGANYDLHTLDTLSKNAKNTLKKKGKRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS74</accession>
    <name>A0A2S2KS74_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_12080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877057.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS74"/>
    <dbReference type="GeneID" id="76209297"/>
    <dbReference type="OrthoDB" id="358887at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052164">
        <property type="entry name" value="Anthracycline_SecMetBiosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33993">
        <property type="entry name" value="GLYOXALASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="5"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="137" mass="15071" checksum="7A831919E1F84C36" modified="2018-07-18" version="1">MNIKKVGNVILAVKDIDKSIQFYHELIGLPIKNQRRSWVDLGTSGAMLSLHPASLTAQHVGSSIDNGITIGFLVGDVQSAIDELKEKGVRIHRDIVERDAGKNAVILDPDDYLISLFEPNFEDKDQQTGGYHGFTPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS91</accession>
    <name>A0A2S2KS91_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13000</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS91"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="194" mass="20075" checksum="B421BC3C3B6A7E59" modified="2018-07-18" version="1">MFVNMNGCPGSMINHYDVLAARLPIGTNLKISKITETASQEKFLGEIMNSTAITAIIGIAILTTSTLAFGATISTPLSFNLIGASDNVEVPAPSVNITSIDFIQEVAGNGVIETDAINFSVGNEDPLNSHSYEVCLVIEGPIGIFNPSAGGTPACTTTSSIPANGIITNQLISTTNATAISDLLNISVSVEELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSF6</accession>
    <name>A0A2S2KSF6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation elongation factor EFTu-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_13740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877188.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSF6"/>
    <dbReference type="GeneID" id="76209125"/>
    <dbReference type="OrthoDB" id="30874at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001514">
        <property type="term" value="P:selenocysteine incorporation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF11">
        <property type="entry name" value="SELENOCYSTEINE-SPECIFIC ELONGATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation elongation factor EFTu-like" evidence="1">
        <location>
            <begin position="154"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="306" mass="33750" checksum="82252B67356F4CFD" modified="2018-07-18" version="1">MVLSVNFVVLGKQDIAAEFGKKGTVTDLSLYDRKESDVIKTWVTPNGFPEKIQPLFQAINLAEYVIFHVDKLDKFTGEQIIALDSLKKEKGILSHTFDVDESKLNAMIKGTVVENYAKIDPEKIKEEMDKLEPVSNDDPSEMVIDHCFDVKGVGTVILGKVTHGKIKQYANLKLYPAGINVLIKSIQMHDDPVEESVCPARVGLAVKGVKPDEVGRGDVITNEGAVDVKTEIELNFKKNPFYKNDISENQGCLVNVGLQIKAAKFMSLSPLKLTFEKPIACKKGQIAVILKPESPTIRILGSGIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSI7</accession>
    <name>A0A2S2KSI7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_13180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877142.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSI7"/>
    <dbReference type="GeneID" id="76209181"/>
    <dbReference type="OrthoDB" id="42697at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40730:SF5">
        <property type="entry name" value="HTH CRO_C1-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40730">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="22"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="138" mass="15188" checksum="B65AABA960015EF2" modified="2018-07-18" version="1">MLLPAEIESKTLIPALRAILAKKLAEDHNIREDEISKMLGVTQAAISNYIRGTRGDPSLIAKLLAEKQVAIMIDDLSDNLSSDLAYTPSSLSKFIGLCNYIKSSLLICEIHHNLESNIDEQVCKECENMLLKGPGSVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSJ3</accession>
    <name>A0A2S2KSJ3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Macrolide ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_14170</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877224.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSJ3"/>
    <dbReference type="GeneID" id="76209078"/>
    <dbReference type="OrthoDB" id="31298at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098796">
        <property type="term" value="C:membrane protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000032">
        <property type="entry name" value="Export ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42798:SF6">
        <property type="entry name" value="CELL DIVISION ATP-BINDING PROTEIN FTSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42798">
        <property type="entry name" value="LIPOPROTEIN-RELEASING SYSTEM ATP-BINDING PROTEIN LOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="15"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="238" mass="25996" checksum="961750A6ACB9D5CB" modified="2018-07-18" version="1">MSSMSQTVKQVHGILDVQNVSKVYQTAAGKVVALKNVSFSIPKNQFVSIVGPSGSGKSTLLNMIGALDKPSRGTVLMDGIDIFKLPDNKLAKIRNTKIGFVFQSFNLINRSTVQKNIEMPGIISGMDNATRTKRAQSILKKLGILKKANHKPNNLSGGEQQRVAIGRALINNPAIILADEPTGNLDTATGEDIFQLLTELTKKYSRTVVMITHNVDLAKRTDRSIYMKDGEIEKDVCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSL4</accession>
    <name>A0A2S2KSL4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SHOCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_14240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877231.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSL4"/>
    <dbReference type="GeneID" id="76209071"/>
    <dbReference type="OrthoDB" id="3293at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018649">
        <property type="entry name" value="SHOCT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09851">
        <property type="entry name" value="SHOCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SHOCT" evidence="1">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="127" mass="14302" checksum="5E2520FE4027BC4F" modified="2018-07-18" version="1">MGTSKKSKTSKKGGYIDKFLRRADKAIQEGIKKADDVLDEAVELGEITAKQASKASKEFSEKARKESEILQKKSIERINEGILSAKKMTSSSEEDLRLLEKLGKLRKSGVLTEKEFQEKKKKILSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSL7</accession>
    <name>A0A2S2KSL7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Universal stress protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">uspA</name>
        <name evidence="3" type="ORF">NZNM25_14050</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877217.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSL7"/>
    <dbReference type="GeneID" id="76209094"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF25">
        <property type="entry name" value="USPA DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="7"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="143" mass="15735" checksum="DB526249D7E76BB0" modified="2018-07-18" version="1">MIQKKISKILVPLDGSKNSIRGLEMAITLARQSGAIITGVYSIYAPPHSEFRGIGSVEKALNREVKKIMEDAKVLAAQNGIVFKEKIMRGETGYNIIKLAHGKEKFDIIVIGSRGRSSTKEMFFGSTSNYVIHTSKIPVVIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSM2</accession>
    <name>A0A2S2KSM2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transcription elongation factor NusA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_14710</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877270.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSM2"/>
    <dbReference type="GeneID" id="76209024"/>
    <dbReference type="OrthoDB" id="4111at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="170" mass="19297" checksum="66B64B69C9D04482" modified="2018-07-18" version="1">MILPICGFDAKNSVLCPKCEGKVEAGELTQADIDASIILAKTAKLNKAIENFTLYSCKEFDGNFVLSLAKNDIMIIRQSRTLYRLLQDQFKGKIWLVEADENDKRFIEDLFFPTKILSINAVWAPGGVQKTKAIVSGKWTPKFPIDTEKVIQIVRNARNLDIEIEFEDKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSQ3</accession>
    <name>A0A2S2KSQ3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Dipeptide/oligopeptide/nickel ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_14700</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877269.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSQ3"/>
    <dbReference type="GeneID" id="76209025"/>
    <dbReference type="OrthoDB" id="18209at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000016">
        <property type="entry name" value="Oligopeptide ABC transporter ATP-binding component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43067:SF3">
        <property type="entry name" value="MALTOSE ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43067">
        <property type="entry name" value="OLIGOPEPTIDE/DIPEPTIDE ABC TRANSPORTER, ATPASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="311" mass="34448" checksum="521A1F56A1F03AEC" modified="2018-07-18" version="1">MVFLDIRGLSVKYPSSSGPVYAVDDVDIQLEDGQSIGIAGESACGKSTLGLSIIRMLSSGTIQGNIFFENDSILDMSESDFNENYRWKKISMVFQGAMNALDPVFTINEQFHEILKQHHFEGNSKQLILDAINSVSLDENVLKKYPHELSGGMKQRVVIAMALLLKPKFVIADEPTTALDMLIQAQIIALLKTLKKEGMSFLLITHDLAVLSEIADKIGIMYGGQIVEFGTSEEIYKNPQHPYTQGLLESIPTLKGGSPKYIKGSPPSLLDAPTACRFLERCPLAIEKCKKLPPKLATKTGYVRCWLYEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSR6</accession>
    <name>A0A2S2KSR6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">NH(3)-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="8 10">6.3.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nadE</name>
        <name evidence="12" type="ORF">NZNM25_13820</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>deamido-NAD(+) + NH4(+) + ATP = AMP + diphosphate + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21188"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the NAD synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.5" evidence="8 10"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877195.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSR6"/>
    <dbReference type="GeneID" id="76209117"/>
    <dbReference type="OrthoDB" id="39312at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00333"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008795">
        <property type="term" value="F:NAD+ synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000106">
        <property type="entry name" value="Glutamine-dependent NAD(+) synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00193">
        <property type="entry name" value="NadE_ammonia_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022926">
        <property type="entry name" value="NH(3)-dep_NAD(+)_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010587">
        <property type="entry name" value="PRK13980.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090:SF9">
        <property type="entry name" value="GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8 9" id="KW-0520">NAD</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD/GMP synthase" evidence="11">
        <location>
            <begin position="18"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <begin position="252"/>
            <end position="253"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH34591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="263" mass="29857" checksum="215BD1380D215A16" modified="2018-07-18" version="1">MNQEIINEIKNKDYALITQTLEKFLSDQIEKNHVNGLILGLSGGIDSAVLAYLCKRQLKEKTIALIMPDTLITPKSETEDALKMIALTGIEYKLIDIKPIVNEYSMYVEPNNLAKGNLRARVRANILYYYANSKNYLVLGSSDKSEYLLGYFTKYGDGASDITPIISLYKLQVREIAKYLGVPENVIAKRSSPHLWKEHEAEEELGASYEEIDSILYCLFDKKKSIGETAELTQIDKSLVEKIYSQNKNSEHKRLPAPKPDRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KST2</accession>
    <name>A0A2S2KST2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_14750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KST2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="281" mass="32765" checksum="DC84B544C677F80C" modified="2018-07-18" version="1">MNTKFFILIIFIAIVASFVGFQFFLDENYMSIQTQDEIQKTKLDIKSLFNIYGYEDNFRIKDGKKIWNDPKFELNPENNDLYLELNHSSIENAVVIYPIFTASAYSEPGFYTFFRGECGQECLTDEIDSEYSLDFVASGNGYQVLNLLGYPIITDIDVDQNPAILEKYDKVILLHNEYVTKKEFDAITNHPKVLYLYPNALYAEVEADYEKNTISLIRGHNFPEPEIVNGFDWEFDNSNLEYDMDCKDMGFDRIANGWMLNCYPENPIHQSKVLLQMIKDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KST3</accession>
    <name>A0A2S2KST3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phasin family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_14020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877214.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KST3"/>
    <dbReference type="GeneID" id="76209097"/>
    <dbReference type="OrthoDB" id="7075at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="15923" checksum="13A9E65ABC45C32B" modified="2018-07-18" version="1">MSKNETTGNSKDLFSAYQENIDKIFNGIKQSVPQYHQSITNVQQEYLQAYENIVDSTITLQKEYVKKAGIAANIPETTLKVIHDTTEEFVKATSIQNQVALATIDATQQNIKTFNDNAKSFADLNRNILQSWISVFTTKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KST6</accession>
    <name>A0A2S2KST6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Non-canonical purine NTP pyrophosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_15230</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877361.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KST6"/>
    <dbReference type="GeneID" id="76208465"/>
    <dbReference type="OrthoDB" id="372108at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047429">
        <property type="term" value="F:nucleoside triphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009143">
        <property type="term" value="P:nucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00515">
        <property type="entry name" value="HAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002637">
        <property type="entry name" value="RdgB/HAM1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00042">
        <property type="entry name" value="RdgB/HAM1 family non-canonical purine NTP pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067:SF9">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE/HAM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01725">
        <property type="entry name" value="Ham1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="182" mass="20816" checksum="6593510800152426" modified="2018-07-18" version="1">MQKSFDLFFVSSNDHKYLEARKILHGFGIALGFLKSDLEEVQSNTLNEIALRKAKNAFSKFKKPIIIEDDGLFIDSLKGFPGPYSSYVFKTIGNDGILNLLKNNRKAKFVSIITYCDESVLQSFDGKLDGTISRSQKGKGWGYDPVFIPKNSKKTFAEMNDKNNFSHRYKALKKFSNWYLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSU5</accession>
    <name>A0A2S2KSU5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1059 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_15100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877304.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSU5"/>
    <dbReference type="GeneID" id="76208986"/>
    <dbReference type="OrthoDB" id="9023at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009409">
        <property type="entry name" value="DUF1059"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06348">
        <property type="entry name" value="DUF1059"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="54" mass="6269" checksum="016FBD60AE8CC8A3" modified="2018-07-18" version="1">MVKISCNNYGFDCSFEINGNTNEVIEKYQKHSIDEHGIEYSTEGINQLLLRMKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSW9</accession>
    <name>A0A2S2KSW9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-(apurinic or apyrimidinic site) lyase</fullName>
            <ecNumber evidence="2">4.2.99.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">NZNM25_15340</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-1 OGG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877369.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSW9"/>
    <dbReference type="GeneID" id="76208455"/>
    <dbReference type="OrthoDB" id="14922at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008534">
        <property type="term" value="F:oxidized purine nucleobase lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012904">
        <property type="entry name" value="OGG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052054">
        <property type="entry name" value="Oxidative_DNA_repair_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10242">
        <property type="entry name" value="8-OXOGUANINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10242:SF2">
        <property type="entry name" value="N-GLYCOSYLASE_DNA LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07934">
        <property type="entry name" value="OGG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="10">
        <location>
            <begin position="108"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="287" mass="33863" checksum="26944318209EED1D" modified="2018-07-18" version="1">MKKLLYNMQKYSTINVENSINSGQVFLWKKNENYWYGVNGQDVLRVDEKGIIDSFHNSRTDFFRKKDNTESIIKSISKDKTVKNAVKKYPGLRILRQDPFQCLISFIVSSNSNIQKIKTSLENLSKKFGTKVEYENQEFFLFPKPEKLAKASINEIKSCGVGYRDKFIKEAARMILLEKIDLELLKKCKYEECKQSICTIPGVGNKVADCVMLFSLEKLEAFPLDRWMIRILEKYYFDKFQLDTKTVTEKQYDTLHEKIVNHFGPFAGYSQQFLFKMERENYQKKWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSZ1</accession>
    <name>A0A2S2KSZ1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_14820</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877280.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSZ1"/>
    <dbReference type="GeneID" id="76209013"/>
    <dbReference type="OrthoDB" id="73567at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030554">
        <property type="term" value="F:adenyl nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043168">
        <property type="term" value="F:anion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08235">
        <property type="entry name" value="iditol_2_DH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050129">
        <property type="entry name" value="Zn_alcohol_dh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401:SF2">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="5">
        <location>
            <begin position="7"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="342" mass="37787" checksum="43E558F8DFA6CBF0" modified="2018-07-18" version="1">MKTASVKEPSVITVDETERPSLGNGDVLVQMNACGICGSDLEKVFGQYGQPSMRLGHEPSGIILDVGSDVVEFKKGDRVFTHHHVPCYDCHLCNHGNETMCPKYYETNLFPCGLSEEYVVPEWNVSHGGILKISDSISFEEAAMIEPLACCVRAWSKFHYHEHDSTAIFGVGPTGMMHVMLAHVKKFSKIFCFDVNGFRLDFAKKFNITDSINSMDENRKQKILDGTGGRGVDVAIVATSSLKALEDAIDMVRKGGTVMMFGVPSKDSQLNLDMSKIYSKEITLVTSYAASDKDTKEALELIESSKIDVKQLITHTYSIVDSQKAFDHAHTGENAMKIIITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT05</accession>
    <name>A0A2S2KT05_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dihydropteroate synthase</fullName>
            <ecNumber evidence="4">2.5.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_15650</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(7,8-dihydropterin-6-yl)methyl diphosphate + 4-aminobenzoate = 7,8-dihydropteroate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19949"/>
            <dbReference type="ChEBI" id="CHEBI:17836"/>
            <dbReference type="ChEBI" id="CHEBI:17839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="EC" id="2.5.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.15" evidence="4"/>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_370684794.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT05"/>
    <dbReference type="GeneID" id="76208422"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004156">
        <property type="term" value="F:dihydropteroate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="entry name" value="Dihydropteroate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045031">
        <property type="entry name" value="DHP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006390">
        <property type="entry name" value="DHP_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01496">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941">
        <property type="entry name" value="FOLATE SYNTHESIS PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941:SF1">
        <property type="entry name" value="FOLIC ACID SYNTHESIS PROTEIN FOL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="Dihydropteroate synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pterin-binding" evidence="9">
        <location>
            <begin position="26"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="299" mass="32658" checksum="5524395C5F277D26" modified="2018-07-18" version="1">MTQIALDLINHVAKLANVGVGGKNPVRIMGILNTSPESFYKKSIHTSTIKIKNYIKEMENAGADFIDVGGMSTAPYLPTMISEKTESKRVLSAIKIIQNVSNLPISVDTCRAKVAKDALENGVEIINDISGLKYDDEMKKVVSKFSPSLILCAYDSKTVLGNPITSTKKLLRESLKIAKSSHISSEKIVLDPAIGFFRKTGQGQFFTKIKSDWVERDLSIIQNLNLIKQSFPILISVSNKSFLGRILGKENPADRLFGSIAAETISVVNGVDVIRTHNVQATKDAITVASNLSKQHKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT64</accession>
    <name>A0A2S2KT64_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycoside hydrolase family 42 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_16240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877460.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT64"/>
    <dbReference type="GeneID" id="76208361"/>
    <dbReference type="OrthoDB" id="8819at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="369" mass="41204" checksum="F119139B44E62168" modified="2018-07-18" version="1">MNKKILGIIAGIVVIAIVSTVLVMPNSEINTRTNEKIGLVINSPSQSVTLQNLDEIYSEAASSGIGRSNVYLFWNLVEPVRDKFDWQQSDVLMSFNKKNNLKVTLYFSVINGETLGPFPSWIGKPPIISIGEDRVVSVLDAILSRYDIIDTVILAGETESQFRFNEQNIPVYKDLFNGVYEKTKEKHPDVKIGNAFALHHVINKNLQYIVTDLAVGDFIAFSYSPVDTLNDIIKTPEQAIGDLEQVFDLAGDKKVGIFELSWSTADFVGGSDSSQTEFLEKSFDFYAANESELEFFTWYRQYDKPEGTCTFEEQEVGEDTLTVGGSGFGSSEHVIERLNQYVCSAGLINSDGTPKSSWIEFKKQVDMTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT75</accession>
    <name>A0A2S2KT75_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Imidazole glycerol phosphate synthase subunit HisH</fullName>
            <ecNumber evidence="10">4.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">IGP synthase glutaminase subunit</fullName>
            <ecNumber evidence="10">3.5.1.2</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">IGP synthase subunit HisH</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">ImGP synthase subunit HisH</fullName>
            <shortName evidence="10">IGPS subunit HisH</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">hisH</name>
        <name evidence="13" type="ORF">NZNM25_15480</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the synthesis of IGP and AICAR. The resulting ammonia molecule is channeled to the active site of HisF.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 15">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 10">Heterodimer of HisH and HisF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.10" evidence="10"/>
    <dbReference type="EC" id="3.5.1.2" evidence="10"/>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877381.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT75"/>
    <dbReference type="GeneID" id="76208440"/>
    <dbReference type="OrthoDB" id="33401at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01748">
        <property type="entry name" value="GATase1_IGP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00278">
        <property type="entry name" value="HisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010139">
        <property type="entry name" value="Imidazole-glycPsynth_HisH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01855">
        <property type="entry name" value="IMP_synth_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42701">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE SUBUNIT HISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42701:SF1">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE SUBUNIT HISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000495">
        <property type="entry name" value="Amidotransf_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="6 10" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="12">
        <location>
            <begin position="6"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10 11">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="active site" evidence="10 11">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <feature type="active site" evidence="10 11">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000495-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH34757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="201" mass="22137" checksum="1B7BED94FAC54023" modified="2018-07-18" version="1">MVSVAIFDYGAGNIFSLKNSLEKAGASVDVITNFDEPNKYSGLLLPGVGNFDPAINSIRNYSKTEFRDYVGDMPVLGICLGMEMFFEKSEEGKESGLNVIDGEVIVLPPTLKVPHMGWNDLEIKKPGKILEGVDDGSWVYFVHSYRVKPSSDDVITAVSDYGIKVPAVVEKDNFIGTQFHPEKSSTVGKMMIKNFLDVCKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTA1</accession>
    <name>A0A2S2KTA1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Acetolactate synthase</fullName>
            <ecNumber evidence="10">2.2.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">NZNM25_14790</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>2 pyruvate + H(+) = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="10">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the TPP enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="10"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTA1"/>
    <dbReference type="OrthoDB" id="6837at2157"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02015">
        <property type="entry name" value="TPP_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000008">
        <property type="entry name" value="Acetolactate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000007">
        <property type="entry name" value="Acetolactate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012846">
        <property type="entry name" value="Acetolactate_synth_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039368">
        <property type="entry name" value="AHAS_TPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000399">
        <property type="entry name" value="TPP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00118">
        <property type="entry name" value="acolac_lg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968:SF13">
        <property type="entry name" value="ACETOLACTATE SYNTHASE CATALYTIC SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="THIAMINE PYROPHOSPHATE ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00187">
        <property type="entry name" value="TPP_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="13">
        <location>
            <begin position="12"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="11">
        <location>
            <begin position="201"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="12">
        <location>
            <begin position="398"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GBH34688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="570" mass="61727" checksum="7737FED7B6415D6C" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTD1</accession>
    <name>A0A2S2KTD1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histidine kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_15090</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTD1"/>
    <dbReference type="OrthoDB" id="8127at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00075">
        <property type="entry name" value="HATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065:SF10">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065">
        <property type="entry name" value="SENSOR HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="151"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="24"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="358" mass="41086" checksum="960E1F742DE64179" modified="2018-07-18" version="1">MLVDNPMERDPVLRDLTEISVNALKDLELARKELKENDLRMQEMNKLANERVNEMSRVNQQLQDKISFVETMTKSIQEKNEHLEKELKITETEKINYSKLNSLLKEDLTKVIKKEKELSVKQIFLERKVKEQAENLLKGEKMSIIGTFTSRLAHDIRNPLSKLKMSHDILCNSPNMPVLEKIKHQQRIESAISNMTHIIEDVLEFVRMSELNMHETSLKTIIKSSIEGIHIPPSVKLEIKGDDRMALCDSRKLEAVFINLITNAIESIRGKGFVTIFIKDSKENIEICFEDSGSGVIPRLQEKIFEPMFTTKQHGTGLGLAICKMIVEQHGGRLIYKNTPSTFSVFVPKINNSKMVTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTF4</accession>
    <name>A0A2S2KTF4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Adenylate kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_15290</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877366.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTF4"/>
    <dbReference type="GeneID" id="76208460"/>
    <dbReference type="OrthoDB" id="8730at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020618">
        <property type="entry name" value="Adenyl_kinase_AK6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595:SF0">
        <property type="entry name" value="ADENYLATE KINASE ISOENZYME 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595">
        <property type="entry name" value="POS9-ACTIVATING FACTOR FAP7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13238">
        <property type="entry name" value="AAA_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="182" mass="20681" checksum="2DDB1440BEFAD3C3" modified="2018-07-18" version="1">MSLVITGNPGVGKHTIAEKIAQRLGLVIFDINKIAKDAGLFEENQDTNDVDTEILEKIIAEKISENNLIIGHLAPYVLEKNQVKKMIILRRSPYDLIKVYKERRYPDEKIRENTGSEILGIISHDAISKFEEKTIQIDITGKTIQEAIEKVMVMISGNEGDEKVDWLDLVTKNNDLQKFFVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTG5</accession>
    <name>A0A2S2KTG5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Imidazole glycerol phosphate synthase subunit HisF</fullName>
            <ecNumber evidence="11">4.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">IGP synthase cyclase subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">IGP synthase subunit HisF</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">ImGP synthase subunit HisF</fullName>
            <shortName evidence="11">IGPS subunit HisF</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">hisF</name>
        <name evidence="13" type="ORF">NZNM25_15460</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 11">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 11">Heterodimer of HisH and HisF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11 12">Belongs to the HisA/HisF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.10" evidence="11"/>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877379.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTG5"/>
    <dbReference type="GeneID" id="76208442"/>
    <dbReference type="OrthoDB" id="6261at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04731">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000006">
        <property type="entry name" value="Imidazole glycerol phosphate synthase subunit HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01013">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004651">
        <property type="entry name" value="HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050064">
        <property type="entry name" value="IGPS_HisA/HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00735">
        <property type="entry name" value="hisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235:SF2">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE HISHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE SUBUNIT HISF/H IGP SYNTHASE SUBUNIT HISF/H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11 12" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="11">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH34755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="267" mass="29140" checksum="7DA8FC5D47015F8F" modified="2018-07-18" version="1">MTLTKRIIPCLDVKNGRVVKGLHFESIKDAGDPVELARKYSDEGADELVFLDITASDEQRETIKDLVRRVASVIDIPFTVGGGVKSLDDARNILLNGADKVGINTGAIKNPQIITELMELFGRQCVVVAIDAKRNYDLKDGVTVFSENGQSFWYEVFIYGGKKETGIDVIQWAKKVDELGAGEILLTSIDRDGTKDGYDILLTTSIVDSVSIPVIASGGCGKPSDMVDVFEKSNVDAALAASIFHYETHGVKGVKTYLKEKEIPVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTI7</accession>
    <name>A0A2S2KTI7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Recombinase RecA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_17370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866929.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTI7"/>
    <dbReference type="GeneID" id="76208248"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="203" mass="23314" checksum="C6231A500E608A17" modified="2018-07-18" version="1">MMVLDKSSELILDNSFEHIKNEFESNIPSLVLCSEPFHKAEFLYRLMNSIEILTIYVDLDLLYTGYVESGMIKKKNNVIIFHPEKENWEKKLVEIISKVSKEKFLVVIDSFNGVYNMFDDLESARFVNSCIMLLSSLANQTNSSVIVTGMARRKENLGWVISPGGKQIIKSKKTGVYFIKKNPNYMIFTMEGSNSKAFKIEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTK5</accession>
    <name>A0A2S2KTK5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">tRNA (guanine(10)-N(2))-dimethyltransferase</fullName>
            <ecNumber evidence="12">2.1.1.213</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">tRNA:G10 dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">NZNM25_17500</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the adenosylmethionine-dependent methylation of the exocyclic amino group (N(2)) of guanosine at position 10 of various tRNAs. Acts via a two-step process that leads to the formation of either N(2)-monomethyl (m(2)G) or N(2)-dimethylguanosine (m(2)(2)G).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>guanosine(10) in tRNA + 2 S-adenosyl-L-methionine = N(2)-dimethylguanosine(10) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43124"/>
            <dbReference type="Rhea" id="RHEA-COMP:10355"/>
            <dbReference type="Rhea" id="RHEA-COMP:10358"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
            <dbReference type="EC" id="2.1.1.213"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the methyltransferase superfamily. Trm-G10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.213" evidence="12"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877558.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTK5"/>
    <dbReference type="GeneID" id="76208235"/>
    <dbReference type="OrthoDB" id="7080at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160101">
        <property type="term" value="F:tRNA (guanine(10)-N2)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000251">
        <property type="entry name" value="Putative RNA methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RlmKL-like_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053943">
        <property type="entry name" value="RlmKL-like_Mtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001091">
        <property type="entry name" value="RM_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14911">
        <property type="entry name" value="THUMP DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14911:SF13">
        <property type="entry name" value="TRNA (GUANINE(6)-N2)-METHYLTRANSFERASE THUMP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00508">
        <property type="entry name" value="S21N4MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01261">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Ribosomal RNA large subunit methyltransferase K/L-like methyltransferase" evidence="14">
        <location>
            <begin position="155"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GBH34959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="315" mass="36285" checksum="061DCBC5651986F7" modified="2018-07-18" version="1">MPENFFILSKDHLELATDEIIALSKMYDRFSKIKIISNLIIIQSKTNWEKITKRASFVKISGQILRKMSGLFLDEENLGVLKNAKTFVCRIINLSLNQFNISELERSMGDMISKFSNAKVKLEDPDITVYLIFTDKENFFGFSKRIKEENKPKKVTKYPHELDWKLTRAMINLAGMKEGETVCDPFCGTGTTLLEAESMGINAIGLDFDEKMCAISKENLKVNGYKSRILNTDFQELVEISDEFDGIVTDLPYGRSSKTSENPEVILKKFFSILPKRKKVAVMYKKELDNKLKLKGLKKYEIYRHKSLTRTILIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTL0</accession>
    <name>A0A2S2KTL0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_15890</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877426.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTL0"/>
    <dbReference type="GeneID" id="76208398"/>
    <dbReference type="OrthoDB" id="1486at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="221" mass="25577" checksum="3B26ED819250EFF2" modified="2018-07-18" version="1">MEITQNECFIELSSFDDLARYVCAFQEYPRRVYSQEFKGSRIISSSLTLANTLLIFYAPLPKFGRYVSYRVNGDKEICDVVESTKNISNYAPIVHMESEISPLPAESKKISDVFHPIQVRDLGSLARLTYNPEFPDESNLTLYALQHKESWVIGYITSLEMDEVYYNFNYVKLYSEPSKHFIKYQGNLGKEPEFSNSIEHGFSYLPIIKIKKNPHIFGFST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTM7</accession>
    <name>A0A2S2KTM7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_17950</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877598.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTM7"/>
    <dbReference type="GeneID" id="76210326"/>
    <dbReference type="OrthoDB" id="5605at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="69" mass="7949" checksum="DB3394E64A08B9A1" modified="2018-07-18" version="1">MAYCIGECKKYKALKPLQIGRYAAGQKRCNYCEIFVEIDGIICPCCKSQLRCLPRSRKGKEKYLAQIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTP2</accession>
    <name>A0A2S2KTP2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3-oxoacyl-[acyl-carrier-protein] reductase FabG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">fabG</name>
        <name evidence="2" type="ORF">NZNM25_18040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877606.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTP2"/>
    <dbReference type="GeneID" id="76210317"/>
    <dbReference type="OrthoDB" id="24596at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005559">
        <property type="entry name" value="PRK07231.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43943">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE (SDR FAMILY) MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43943:SF2">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="247" mass="26758" checksum="F92D0A613FE05B98" modified="2018-07-18" version="1">MKLSGKVAIVTGGSRGIGFATARILSENGATVVLTAKNQERLEKASSEIPNSLGIAADIKNKNDVKNVVSKTVAKFGQIDILVNNAGIFPKIKELHMIDEDEWNEILDVNLTGQFRFTKEAIPYLQKTSGSIINISSDAGLKAYQGFNADAYSATKAALILLTKCWALEYAKDKIRVNCICPGVVDTDMTKPFLKTQKDKEFMDNEHPIGRIGQPEEVGKAVLYFASDDASWTTGAILAVDGGESIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTR3</accession>
    <name>A0A2S2KTR3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_18240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877623.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTR3"/>
    <dbReference type="GeneID" id="76210297"/>
    <dbReference type="OrthoDB" id="46222at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="410" mass="47171" checksum="37F8057DF2798923" modified="2018-07-18" version="1">MLKIGEFIYPWGSGHYSRMMRLNEVLGDHIKEEFEVHFSSKDHVYEKLLKKFPEQKEKIHEILMPTPIDGKFGPSVTMSLMNLLLPISKNPPLVRQIANYLREERKLYNKERFDLVINDGDMGSNIIAKNRNIPSLFITNQFRPKLYNSRAYLYPSLIFVAKQIAKATKILVADSPPPYTMCEYNLNLTDDVKEKVVYVGQFTSKQIKNEEKTDLEKLVENNEFGYWMRTGNKSTNDGTGQRYEKVFHQDNMRNEKRVISHARNDPNIDSVIGKNGNKYSVSEALDKKVDWIQIDIGFLSEQEKDTILNLCKYAVVNGSHTVMGEIMGGKSKPIIGIPIYDEHTNNILWAQEKNLGILATKTTQVIDAIYKIKNNYEEFEGNLDEFSKKFVPNGAENSAKIAAEILEEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTT2</accession>
    <name>A0A2S2KTT2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_16660</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877491.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTT2"/>
    <dbReference type="GeneID" id="76208319"/>
    <dbReference type="OrthoDB" id="4272at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.50">
        <property type="entry name" value="ATP synthase (C/AC39) subunit, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036079">
        <property type="entry name" value="ATPase_csu/dsu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002843">
        <property type="entry name" value="ATPase_V0-cplx_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050873">
        <property type="entry name" value="V-ATPase_V0D/AC39_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044911">
        <property type="entry name" value="V-type_ATPase_csu/dsu_dom_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38682">
        <property type="entry name" value="V-TYPE ATP SYNTHASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38682:SF1">
        <property type="entry name" value="V-TYPE ATP SYNTHASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01992">
        <property type="entry name" value="vATP-synt_AC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103486">
        <property type="entry name" value="V-type ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="38693" checksum="24D3E6894BDD1129" modified="2018-07-18" version="1">MGGSKNVYASVKAYSKRGKLLTKADFQTLAESRDLEELMTRIKNTVYADSVADVQKPYTSQKIESALRSKLADIHYSIAKTSGNSGVLDAYYMKFIIANLKLILKGKVLGKPQEEIETHVNLHAEELIKQRDIVIKALVSKDLEEAVASLNSVKFGEEIAKAAALYNEKKNVQVFDTYFDKILYQHLAGAMKNYSDKEATKLVSMDIDFYNILSVIRGKFWGLKEEQIQDLVISTSPPARELLGRMMAAATARDAFNELSSTKYKDLVPQVENELDAIAEFERAFEMAIYSASLRSFTKMFSFATIVGITKLTSFEIRNLAAIAFAIEQKIPTDITMSKLILEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTT8</accession>
    <name>A0A2S2KTT8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Water stress and hypersensitive response domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_16690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877494.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTT8"/>
    <dbReference type="GeneID" id="76208316"/>
    <dbReference type="OrthoDB" id="11878at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117070">
        <property type="entry name" value="LEA14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="185" mass="20375" checksum="905AB7499A55B256" modified="2018-07-18" version="1">MNPKIFVGVAVAALALILGGILLVGPTMVISSQDDSLDTTPIVQQVKPLEIELEDISVAKISERSATIDIAFKISNPNPRSVIVQTMDYQLFETGYSDFEQISGGEIGSRPEGMVEFGSNYYTLLGENSIILRDTVVLRNTGNTPDLWKAFEDGTNTWRVSGDVFYNLSSMTSGQENELHFEFTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTU7</accession>
    <name>A0A2S2KTU7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_18650</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877690.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTU7"/>
    <dbReference type="GeneID" id="76210093"/>
    <dbReference type="OrthoDB" id="9837at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052934">
        <property type="entry name" value="Methyl-DNA_Rec/Restrict_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37291">
        <property type="entry name" value="5-METHYLCYTOSINE-SPECIFIC RESTRICTION ENZYME B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37291:SF1">
        <property type="entry name" value="TYPE IV METHYL-DIRECTED RESTRICTION ENZYME ECOKMCRB SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="375"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="760" mass="88330" checksum="D5571CB824F6159B" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTW9</accession>
    <name>A0A2S2KTW9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glycerol-1-phosphate dehydrogenase [NAD(P)+]</fullName>
            <shortName evidence="11">G1P dehydrogenase</shortName>
            <shortName evidence="11">G1PDH</shortName>
            <ecNumber evidence="11">1.1.1.261</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Enantiomeric glycerophosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">sn-glycerol-1-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">egsA</name>
        <name evidence="15" type="ORF">NZNM25_16990</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol 1-phosphate (G1P). The G1P thus generated is used as the glycerophosphate backbone of phospholipids in the cellular membranes of Archaea.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>sn-glycerol 1-phosphate + NAD(+) = dihydroxyacetone phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.261"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>sn-glycerol 1-phosphate + NADP(+) = dihydroxyacetone phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21416"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.261"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11 12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the glycerol-1-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.261" evidence="11"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTW9"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106357">
        <property type="term" value="F:glycerol-1-phosphate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106358">
        <property type="term" value="F:glycerol-1-phosphate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08173">
        <property type="entry name" value="Gro1PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00497_A">
        <property type="entry name" value="G1P_dehydrogenase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023002">
        <property type="entry name" value="G1P_dehydrogenase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032837">
        <property type="entry name" value="G1PDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016205">
        <property type="entry name" value="Glycerol_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43616">
        <property type="entry name" value="GLYCEROL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43616:SF5">
        <property type="entry name" value="GLYCEROL DEHYDROGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13685">
        <property type="entry name" value="Fe-ADH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000112">
        <property type="entry name" value="Glycerol_dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11 14" id="KW-0520">NAD</keyword>
    <keyword evidence="5 11" id="KW-0521">NADP</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9 11" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11 12" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="11 14">
        <location>
            <begin position="91"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 14">
        <location>
            <begin position="113"/>
            <end position="116"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>glycerol</name>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 14">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>glycerol</name>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>glycerol</name>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>glycerol</name>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000112-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000112-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000112-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GBH34908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="342" mass="37117" checksum="90B94DB453590840" modified="2018-07-18" version="1">MELPRQIVVGEKNISEFGGFLHNLTKPKKVSLISGIHVKKILKEKIEKSLKSKKIQFVWHLSKDNQISSLNKIEKDVKKDHSDIVAGIGGGRSVDAAKMVSFNLDIPFVSVPTAASHDGMASPFVSVKTDKPHSIVASAPLGVFVDIDIIKKAPTRLLSSGCGDLIANIIAVKDWQLGHEKTGEYYGRYSADLALMSANIVIENSRKYAKNGLDARVIVEALISAGVASCIAGSSRPCSGAEHLFSHALDKIAPGKGLHGEKCGLGSIMISKLQGQDWRKIAKALKDLGAPITARQIGLKEEQIIDALIIAQELRPERYTILKEIEMTERKAMNLAKSTKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTY4</accession>
    <name>A0A2S2KTY4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fibronectin-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_18320</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTY4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.310.10">
        <property type="entry name" value="ibrinogen binding protein from staphylococcus aureus domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051608">
        <property type="entry name" value="RQC_Subunit_NEMF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041120">
        <property type="entry name" value="RqcH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239">
        <property type="entry name" value="NUCLEAR EXPORT MEDIATOR FACTOR NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239:SF6">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05833">
        <property type="entry name" value="NFACT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NFACT RNA-binding" evidence="2">
        <location>
            <begin position="385"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="343"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="600" mass="67550" checksum="2D6845B07D763228" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTZ4</accession>
    <name>A0A2S2KTZ4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_18870</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877709.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTZ4"/>
    <dbReference type="GeneID" id="76210114"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="6"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="142" mass="15751" checksum="153559BE452486CA" modified="2018-07-18" version="1">MKKSLYMNILVPLDGSKYSEKALIHACEMAKNYQSRLILVYVVEKSIPINLLDRKEYLEILRKFGNKVLTKGKETATKRGVDSKIVMKEGNVSSEIIKLAKKEQCNMIIVGSKGLGSAARLFLGSVSNKLANNSTCSILIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU14</accession>
    <name>A0A2S2KU14_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Blue (type 1) copper domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_17390</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877547.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU14"/>
    <dbReference type="GeneID" id="76208246"/>
    <dbReference type="OrthoDB" id="11836at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000923">
        <property type="entry name" value="BlueCu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052721">
        <property type="entry name" value="ET_Amicyanin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507:SF1">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00127">
        <property type="entry name" value="Copper-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Blue (type 1) copper" evidence="4">
        <location>
            <begin position="43"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="330" mass="37457" checksum="562C2127E8AF27EC" modified="2018-07-18" version="1">MKFLIFSIILGILVSTSTVFADEYVIDIPLGAYNPELNTPAEVWYDPPQLYATVGDTITWYNDDKEGHTVTSGEGSGRFGWMSDNFGTPNGIFDSGRFMPGESWSYKFEESGTFSYYCTIHPWMEGVLIIEKAIPNYPHDATGAKIEFPLLQYTPDLGIEVNLSWDPPVIKTHEKVQFVYQFYDPQTNSNLAEMKYNFVIFQNGKEIFRDEGLSQIGGDYRNFVFSDSGSIIIRIEGIHTPSIFAEESVTVFGNIESKEQRSVDFTTAVYDNPEKTTHETYHTKPAQRLTTYYELMLVIILVPGILFIGAMIWLKKKPNTRQENSGAVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU50</accession>
    <name>A0A2S2KU50_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_19500</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877760.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU50"/>
    <dbReference type="GeneID" id="76210180"/>
    <dbReference type="OrthoDB" id="46222at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04186">
        <property type="entry name" value="GT_2_like_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43179:SF12">
        <property type="entry name" value="GALACTOFURANOSYLTRANSFERASE GLFT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43179">
        <property type="entry name" value="RHAMNOSYLTRANSFERASE WBBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="5">
        <location>
            <begin position="18"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH35159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="356" mass="41271" checksum="B969EED3DE02F686" modified="2018-07-18" version="1">MIEINKDKHTESHNPLVSIIILNYNAGNLLVDCINSIQKSTYTNFEIILVDNVSKDNSHIICKEKFPTIKLIENSENLGYCEGNNVGLRKANGEFMMVLNPDTIVDSQWLEELLNAYQTNGEGIYQPKFLAITDHSMLLSTGNMIQIFGFGFSRSKGNMDKKQFENIEKIDYVSGTCLFTSKKVLKKIGLFDPFLFAFHDDLELCWRAALIKINSFYVPKSIVYHPIEGTSFKWSPLKFKLMERNRKYCLLTLYDRKTLLKMIPGLFLVDIAVFFFYLKKGLVKMKISADVEILQNFKIINQKYSQNQKIKIISDKEIIQHLKNKVDVPNWIVDTKTNHFFNSFLNRISKITRMII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU76</accession>
    <name>A0A2S2KU76_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_18980</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU76"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="65" mass="7466" checksum="5175CE7B650899E8" modified="2018-07-18" version="1">MLGFVAIALLVIGLVGQAFEMRKIRLTTYKDEDLGSTNIFMNKKNLKWYIVLGIGIVLWYVAERM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU95</accession>
    <name>A0A2S2KU95_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sirohydrochlorin cobaltochelatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_20140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877822.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU95"/>
    <dbReference type="GeneID" id="76210248"/>
    <dbReference type="OrthoDB" id="11653at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03416">
        <property type="entry name" value="CbiX_SirB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1400">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002762">
        <property type="entry name" value="CbiX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050963">
        <property type="entry name" value="Sirohydro_Cobaltochel/CbiX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33542">
        <property type="entry name" value="SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33542:SF3">
        <property type="entry name" value="SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01903">
        <property type="entry name" value="CbiX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53800">
        <property type="entry name" value="Chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="28727" checksum="F5359B48F9D5E613" modified="2018-07-18" version="1">MKRGLLLIDRGSREREASEELEAICQGIKKRGDYVFTDFCFLEVEPPYIEDGISKCLKEDIDSLTIVPYFLYPGKKVKNAVTDVMKFQKDTNVKFVVTKPMSMHKTLVDIVENRISTTLEENNVELSKKEVDVMIIGHGSKDPNAQMSLNYIVNELKDLYRNVSRCWLEIEQPDIVEGIKKCEKDNPKVLIIVFYFLHEGAHVKTDINNDLIPALENSKMKKTFITKHLGSDQKMIDLILERAKEVENAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUB5</accession>
    <name>A0A2S2KUB5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nucleotidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_19970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877802.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUB5"/>
    <dbReference type="GeneID" id="76210230"/>
    <dbReference type="OrthoDB" id="6862at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009264">
        <property type="term" value="P:deoxyribonucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010708">
        <property type="entry name" value="5'(3')-deoxyribonucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052419">
        <property type="entry name" value="5_3-deoxyribonucleotidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35134">
        <property type="entry name" value="NUCLEOTIDASE YQFW-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35134:SF2">
        <property type="entry name" value="NUCLEOTIDASE YQFW-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06941">
        <property type="entry name" value="NT5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="6"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR610708-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="179" mass="20808" checksum="D7998059171E29C5" modified="2018-07-18" version="1">MKIALDVDGVLADVIVPWLNYNNTIRPKISKNDITDWDFWKNHEINPYDFYAELSSCWKNWNSIPPTEENLSSVTKNLLEIGQVDIVTARERSTDSFVKNWLNHHNISFDNYVSVIDGPMKADLDYDVFIDDSPLNALKFLENKKQVILYSQPWNKHISENEIHRISILSEAVEKIKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUC1</accession>
    <name>A0A2S2KUC1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HTH dtxR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_19820</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DtxR/MntR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877790.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUC1"/>
    <dbReference type="GeneID" id="76210212"/>
    <dbReference type="OrthoDB" id="24735at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:001014">
        <property type="entry name" value="Iron (Metal) dependent repressor, DtxR family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050536">
        <property type="entry name" value="DtxR_MntR_Metal-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001367">
        <property type="entry name" value="Fe_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036421">
        <property type="entry name" value="Fe_dep_repressor_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022687">
        <property type="entry name" value="HTH_DTXR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022689">
        <property type="entry name" value="Iron_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238">
        <property type="entry name" value="IRON (METAL) DEPENDENT REPRESSOR, DTXR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238:SF7">
        <property type="entry name" value="IRON-DEPENDENT TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02742">
        <property type="entry name" value="Fe_dep_repr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01325">
        <property type="entry name" value="Fe_dep_repress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00529">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47979">
        <property type="entry name" value="Iron-dependent repressor protein, dimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50944">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH dtxR-type" evidence="5">
        <location>
            <begin position="22"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH35191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="151" mass="17126" checksum="BF172588E3640FB5" modified="2018-07-18" version="1">MDALSDDEETLFVGTAEAEHVEMYLKAIWHIKERGEDVKISTIAKMLNVRQPSVVQMLKKLNSKNLVNYNKAGVKLTEDGERIGASMMRNSRLLEVLMDSALKVEIDEEMVCGIEHHMKKQFTDALCTMLNHPRKCPHDHEIPMGECCKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUE6</accession>
    <name>A0A2S2KUE6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_18690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877694.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUE6"/>
    <dbReference type="GeneID" id="76210096"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="449" mass="53137" checksum="D36BB059505BBC27" modified="2018-07-18" version="1">MYSDLSTKIPLTHSRKSRVIISKADIRNEILKILKKPHTPTELSKHFHQTREAFSRNHLYPMRNEGLIVKVAGSNYYQLTGQKNTIVTIQKQLRSEYEIFKTELFKNWTRNNHSKFEYKNQTRFARIVLGFVTPKFKIHPDNITKENWKEIIPNMVNALIDVANYDVVDGEPSWSNRQAIRHALKYGLGIEISKDEGIKLRISGQKPKEKSSDLHITPEQIVQAKKLLLKHYSIEDYLKFGVKTWTFVRPSTVYLIELAKMDFIDQIVEYVEGTDGVKLTDEKVISYAKFRGDKIYTYVRRICHIEVHENKTHDDFDKYILDPEFVEPLEQFYNTRVSQGKKYLFWEDNDTKFEFKTYDKILTSSVARDNKFFKKILSMIGFKKSDFGLYFRANYGFRHFGIQMWLIATDYNYNLVAEMSHEGTETLKKWYGKRTQKDFQRKIKGIMMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUL1</accession>
    <name>A0A2S2KUL1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">6-hydroxymethylpterin diphosphokinase MptE-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_19290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866939.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUL1"/>
    <dbReference type="GeneID" id="76210159"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002826">
        <property type="entry name" value="MptE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41786:SF1">
        <property type="entry name" value="6-HYDROXYMETHYLPTERIN DIPHOSPHOKINASE MPTE-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41786">
        <property type="entry name" value="MOTILITY ACCESSORY FACTOR MAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01973">
        <property type="entry name" value="MptE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-hydroxymethylpterin diphosphokinase MptE-like" evidence="1">
        <location>
            <begin position="33"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="329" mass="37365" checksum="462C2B95B91E46E0" modified="2018-07-18" version="1">MSRNLSSSKELQKSIEDIMKKKQYENFNEWVTNFASNLPDIWNEKSAKELNSLANQSEKHSAIVIGRGPSINKHEHLKLLAASDYQGTIVCCDGKLADALNAGVTPDKFPKYFVATIDPYPTIKKHYEHEIIDSYGSKIKGIFTIITNPNVVERARQSGIQIHWIHSLFDYNEGIKSFNNLSALMIRAKNNLHGLPGIQTGGNVGTASWFIAWQILKCTTVALIGINHGWEEEDSWETIIYHNNKDEISKGVDRNSPSFNRLFKKIYNPDFNCNCILDPLFQFYSEALKEFIARSPEWVTTINATEGGSIFGERILSMKFSNFLEKYNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUN7</accession>
    <name>A0A2S2KUN7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_19860</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877793.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUN7"/>
    <dbReference type="GeneID" id="76210216"/>
    <dbReference type="OrthoDB" id="10095at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="138" mass="15474" checksum="B40C46F461BE6CAC" modified="2018-07-18" version="1">MVAIDTPASLESFRRFIISSTCKSYAPRSYLEDSEVFAEREDNLGAIYVEAADKVTLKKIRDITFVNARDILGIIYNSKSGNTSLKWRQLRRNNGKVSGEASANSLTNLAESGVLTLDWVENYLKKKTEETKTNEVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNP7</accession>
    <name>A0A2S2KNP7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl29</name>
        <name evidence="5" type="ORF">NZNM25_00270</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875909.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNP7"/>
    <dbReference type="GeneID" id="76209353"/>
    <dbReference type="OrthoDB" id="11736at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_uL29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_uL29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036049">
        <property type="entry name" value="Ribosomal_uL29_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46561">
        <property type="entry name" value="Ribosomal protein L29 (L29p)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="68" mass="7949" checksum="1BB9552932DC5CFB" modified="2018-07-18" version="1">MTRISMKTIKQLNEKDLKSKIQEARSELAKLRVDASKGTLRKESGKLKPLRHDIARMLTRINEMKKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNV2</accession>
    <name>A0A2S2KNV2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_01070</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNV2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050595">
        <property type="entry name" value="Bact_response_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591:SF3">
        <property type="entry name" value="RESPONSE REGULATORY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591">
        <property type="entry name" value="STRESS RESPONSE REGULATOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="99" mass="11045" checksum="715B0C0741FBE257" modified="2018-07-18" version="1">MIGLDVVVTGNNGKDAVVMYEKHKPDLIFVNLIMPEFDGFYAIKNILKNNPDAKIVVVTGDLMAGESYLMNTLKVTAVIYKPFDITIIKRMLTDVFFSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNW3</accession>
    <name>A0A2S2KNW3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7482 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_01100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875984.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNW3"/>
    <dbReference type="GeneID" id="76209439"/>
    <dbReference type="OrthoDB" id="10704at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055905">
        <property type="entry name" value="DUF7482"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24298">
        <property type="entry name" value="DUF7482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7482" evidence="1">
        <location>
            <begin position="137"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="435" mass="46907" checksum="65430443F2498607" modified="2018-07-18" version="1">MNKNLRYIALLAILPLFTTGMATDYFSDADAAKGAGVGTSKYGSSTGICGLQLCSEIPGGKAAWEAGQEKVIPVAPVEESDMEDSMKDDSMMEESDMEDSMKDDSMMEETESDLGTVLRLSRANVPATIPLHQGYYNGEDVYFIITDSSEPTHAEIITKNQGWKVELAPLLKNAPEEALSKTYMFTNGIIGDGVHGFQGEVFTSTPAQADVYSALTSHVHVTWNDGATPRILDSDAMIMDAADNHEITLTPVNVVLNMPQIIWPDGQMMVKEDETLTDTTPYGGGQVLDIDTEEMIVTFIAHRGWGPDGRTIYYIVTDATPSDPAEMMGVVSSPTSASLIANSAAVDLYQFKNGLAGTGPLGFQPGIATGAPGDSNYSPMWRIFVIGWSDPANAQLLETVDDVNAYKEAGLIDIGIARPMDSDHIVNCPFIDPFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNY3</accession>
    <name>A0A2S2KNY3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Ferredoxin--nitrite reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_01840</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876052.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNY3"/>
    <dbReference type="GeneID" id="76209515"/>
    <dbReference type="OrthoDB" id="15347at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.480.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="entry name" value="Sulfite Reductase Hemoprotein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051329">
        <property type="entry name" value="NIR_SIR_4Fe-4S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005117">
        <property type="entry name" value="NiRdtase/SiRdtase_haem-b_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036136">
        <property type="entry name" value="Nit/Sulf_reduc_fer-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006067">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006066">
        <property type="entry name" value="NO2/SO3_Rdtase_FeS/sirohaem_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32439:SF9">
        <property type="entry name" value="BLR3264 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32439">
        <property type="entry name" value="FERREDOXIN--NITRITE REDUCTASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01077">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03460">
        <property type="entry name" value="NIR_SIR_ferr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00397">
        <property type="entry name" value="SIROHAEM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="Nitrite and sulphite reductase 4Fe-4S domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55124">
        <property type="entry name" value="Nitrite/Sulfite reductase N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitrite/Sulfite reductase ferredoxin-like" evidence="8">
        <location>
            <begin position="63"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Nitrite/sulphite reductase 4Fe-4S" evidence="7">
        <location>
            <begin position="132"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Nitrite/Sulfite reductase ferredoxin-like" evidence="8">
        <location>
            <begin position="336"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="Nitrite/sulphite reductase 4Fe-4S" evidence="7">
        <location>
            <begin position="413"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="602" mass="67607" checksum="00D32E26322ECD76" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNY9</accession>
    <name>A0A2S2KNY9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Collagen-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_01810</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNY9"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030020">
        <property type="term" value="F:extracellular matrix structural constituent conferring tensile strength"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF1095">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="135"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="286"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="377"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="436"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="451"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="480"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="493"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="543" mass="53965" checksum="6873E52C1F108D8A" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP17</accession>
    <name>A0A2S2KP17_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01730</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876041.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP17"/>
    <dbReference type="GeneID" id="76209504"/>
    <dbReference type="OrthoDB" id="1510at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="170" mass="19505" checksum="794F62AA2C1BC74E" modified="2018-07-18" version="1">MAKTSEEMKRQVEEFIKITNIKYEDQTEKVREKSKIVEWQFHIGANVIVSKNANRDDRIQVNVNMRFPPEDAKLLVLKNPSFSKAIMEISEICTICGVGHQWMKDKEDIIGLAIFAHVDEHVLDRVSFHNTWDNVARVSGHIQKILRANFSGFPTPNSASDETIDKSMYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP20</accession>
    <name>A0A2S2KP20_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rps3</name>
        <name evidence="10" type="ORF">NZNM25_00260</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds the lower part of the 30S subunit head.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875908.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP20"/>
    <dbReference type="GeneID" id="76209352"/>
    <dbReference type="OrthoDB" id="9126at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02411">
        <property type="entry name" value="KH-II_30S_S3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.20:FF:000001">
        <property type="entry name" value="30S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="entry name" value="Ribosomal protein S3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01309_A">
        <property type="entry name" value="Ribosomal_uS3_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057258">
        <property type="entry name" value="Ribosomal_uS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027488">
        <property type="entry name" value="Ribosomal_uS3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_uS3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005703">
        <property type="entry name" value="Ribosomal_uS3_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003219">
        <property type="entry name" value="PRK04191.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01008">
        <property type="entry name" value="uS3_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760:SF32">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="Ribosomal protein S3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="KH type-2" evidence="9">
        <location>
            <begin position="17"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH33235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="251" mass="28136" checksum="14F56DEF83118E54" modified="2018-07-18" version="1">MSAVKNVIKDNYNMMLLKDYLREAIKDAGFSHAEISKTPVGTRVALHVTRPGIVIGRKGSGIRTLTDKLASDFGLKNPQISVVEIEKPELSPSVMCNRMAAHLERGTAFRRATMWTLKQIMENGAMGVQITISGKLRGDRSAFEKHTAGILPRAGHHAEVIVAEDIAHVKTAMGLIGIRIRIARKDKLIPEFEMKTQKAMKIKQVKDEDTGKMRDETIAETKARTESEQIALEEERMKELETLEEEEARFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP23</accession>
    <name>A0A2S2KP23_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_01250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP23"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="131" mass="14262" checksum="35F7F1A1A8715893" modified="2018-07-18" version="1">MKILCGVNRKQLTKLYNLFQGTFIGLFNYKTYLLFLGASLAISIGLQMVLPFPYGLGAALAIFIVFPLFLRKRYMSRMRGYGGDSGTGGGFFGMGSQGGSGGVRYVCLVCNNKHKGGTCPRCGSKMQRADF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP47</accession>
    <name>A0A2S2KP47_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_02540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP47"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="74" mass="8715" checksum="5DD69A546270A099" modified="2018-07-18" version="1">MQVKFMPEFICPDCGKRLFHENETTLKIEETIHSKFCRKTEGETHSFMHRDPAHMQTFDSERENDTSGTPVLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP56</accession>
    <name>A0A2S2KP56_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_00660</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TrkH potassium transport family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875944.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP56"/>
    <dbReference type="GeneID" id="76209395"/>
    <dbReference type="OrthoDB" id="111943at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32024">
        <property type="entry name" value="TRK SYSTEM POTASSIUM UPTAKE PROTEIN TRKG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32024:SF2">
        <property type="entry name" value="TRK SYSTEM POTASSIUM UPTAKE PROTEIN TRKG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="384"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="417"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="470"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="519"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="552"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="579"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="11">
        <location>
            <begin position="21"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="11">
        <location>
            <begin position="87"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH33275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="604" mass="66407" checksum="74B348CC1840A0A9" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP75</accession>
    <name>A0A2S2KP75_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_02470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876109.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP75"/>
    <dbReference type="GeneID" id="76209581"/>
    <dbReference type="OrthoDB" id="12097at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="160" mass="17158" checksum="D54B35CE177705CC" modified="2018-07-18" version="1">MIQTTKLVFSNFKYVLLASVIFVFMVIGLLILSEYIFLEPYIISHLPVGTELGFVLILMLSALSGLVIPMNIFRINILKSSKQKMGGGIIGSIVGAIAGACSCGPIGFAIISTFGSVGATATAFLTNYDVPIRIIAIVILTVTYFTTVKSLKIECALNTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPA0</accession>
    <name>A0A2S2KPA0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Nitrogen regulatory protein P-II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glnB_1</name>
        <name evidence="1" type="ORF">NZNM25_02910</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876146.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPA0"/>
    <dbReference type="GeneID" id="76209628"/>
    <dbReference type="OrthoDB" id="10960at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30115">
        <property type="entry name" value="NITROGEN REGULATORY PROTEIN P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30115:SF11">
        <property type="entry name" value="NITROGEN REGULATORY PROTEIN P-II HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00340">
        <property type="entry name" value="PIIGLNB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00938">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="109" mass="11790" checksum="B5C8A92B5989888E" modified="2018-07-18" version="1">MLKIEVILGENDVMAISEGLKKIGIGGLTVSKVRGRGKRPGPEIHASKGSEIFTPQFNDKYRIEAIIADAREDEIIGIIKENGRVGKIFVSQILRAVDIATGDEGESTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPB2</accession>
    <name>A0A2S2KPB2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01980</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876065.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPB2"/>
    <dbReference type="GeneID" id="76209530"/>
    <dbReference type="OrthoDB" id="10032at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="93" mass="10849" checksum="1D64E355EC30B3A0" modified="2018-07-18" version="1">MISVKKSFINYAVEQTLVDIGGEPMMMLVSEKLHSKYDCNLENSYKTPRYINDVLIDLYGKSSHNIIDKITETLDEFSHTQEIEKFLLQLNEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPE2</accession>
    <name>A0A2S2KPE2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_01820</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPE2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047558">
        <property type="entry name" value="TPR_END_plus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13431">
        <property type="entry name" value="TPR_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="13"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="47"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="81"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="359" mass="41585" checksum="0177CF9B7AAE1F16" modified="2018-07-18" version="1">MEETSLVDSDYNRKKLFKKGINLMADEKLEDAIVVFEQALRIEPNNIETLMKLGYARFHLEDYGEALKIYDKILDVDVTNPEAWNLKGLVHYEQKNFSKALDSVDKAIETDPTYAMAWYNKACFLSLLNQVPESLEALKRSIEIDVKNARRSIRDKDFSNVRIEEGFKRIQEVVVLESVRQGYHTLGSIVWTTFLDKADAETALRKLLEKGLIVKNEKRDGLSRIPIYDLAPNVAEKLGKEKKGLFGITRKTLPKPVKNLKELSQAIQSVRESIEEEDLEKTIEIFDTFIDPAKSGEQMIENFFDEHREIRLWKIRLKDRGEEYLIENKEKMLVLFGNIEATITKKLRNEISYSADKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPG9</accession>
    <name>A0A2S2KPG9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Phosphoglycerate mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_03610</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the BPG-independent phosphoglycerate mutase family. A-PGAM subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829026.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPG9"/>
    <dbReference type="GeneID" id="76209702"/>
    <dbReference type="OrthoDB" id="52918at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004619">
        <property type="term" value="F:phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16011">
        <property type="entry name" value="iPGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2130">
        <property type="entry name" value="Metalloenzyme domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004456">
        <property type="entry name" value="Pglycerate_mutase_ApgM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042253">
        <property type="entry name" value="Pglycerate_mutase_ApgM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00306">
        <property type="entry name" value="apgM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209">
        <property type="entry name" value="COFACTOR-INDEPENDENT PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209:SF0">
        <property type="entry name" value="METALLOENZYME DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10143">
        <property type="entry name" value="PhosphMutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006392">
        <property type="entry name" value="IPGAM_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloenzyme" evidence="6">
        <location>
            <begin position="10"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="428" mass="46754" checksum="C04B0AD794EF5D4B" modified="2018-07-18" version="1">MDNSDIHMIYVLLDGVGDLPHPDLQGKTPLEAANTPILDKIARNGCIGEVISVGKGIAPESDIAVFNMLGYKFNHAEYAGRGVIEAIGIGIDFKNGDLALRGNYSTLNDEQVIIDRRAGRNIEKEDAEGIAKEIEEKIKFSSPGISVVVSPTIGHRVTVRIRADSQKLSSKITNTDPAYSNIGGMGVAKAVGDFLKIEKCLPLEDDEDSKFTAKIVNEFSEQSIRIMKESQINKKRKELGKKQLSCILLRDAGNKYPDVTPINEKYSMQFSCIVDMPVELGISDVLKMKAFEAGGLTDYEEKARVAAKAMETQNSIYVHLKGPDEFGHDGDAIGKMRNIEEIDQRFFKTLVENIDSNKVAIIISADHSTPCINKGHSDDPVPVLVSADFIQNDGTTRMTEGQAKKGSIGLLQGAEVVTKSLELIKSQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPM4</accession>
    <name>A0A2S2KPM4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cysteine synthase B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_02260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876092.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPM4"/>
    <dbReference type="GeneID" id="76209560"/>
    <dbReference type="OrthoDB" id="10138at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000094">
        <property type="entry name" value="Cysteine synthase protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="1">
        <location>
            <begin position="12"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="330" mass="36143" checksum="F23F6AC4E2D4A721" modified="2018-07-18" version="1">MTTVTDTDVLSRVGNTPLVKLNSLSHNNTEYFAKLEGHNPFGSVKDRAAYWMIKDGEERGILTKGKSIIIEPTSGNTGIALTGIANVLGYKVEIVIPEKASNETKDIIRNLGAKVFETSDDLCPKVGAGTDQSIALATSIASSRPDTYYSPNQYANEANFKGHYVGTGPEIWKQTAGKVTHFFTGVGTGGTITGIGTFLKEKNPNVKIIGCQPQQNHLIQGWRNFEESAKPDLFLKRENVVDDWVSVDNKEAFSVVKEVLEKDRLLISPSSAAVYACMKKYPVEDNACVVGIFADDGRKFKSVYASQNVMSEEEFENALNEAQHMSVLAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPM5</accession>
    <name>A0A2S2KPM5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">6-bladed beta-propeller</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_04210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876299.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPM5"/>
    <dbReference type="GeneID" id="76209769"/>
    <dbReference type="OrthoDB" id="295891at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.150.10.10">
        <property type="entry name" value="Serralysin-like metalloprotease, C-terminal"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001343">
        <property type="entry name" value="Hemolysn_Ca-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001258">
        <property type="entry name" value="NHL_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011049">
        <property type="entry name" value="Serralysin-like_metalloprot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050952">
        <property type="entry name" value="TRIM-NHL_E3_ligases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24104">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NHLRC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24104:SF25">
        <property type="entry name" value="PROTEIN LIN-41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00353">
        <property type="entry name" value="HemolysinCabind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01436">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00313">
        <property type="entry name" value="CABNDNGRPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51120">
        <property type="entry name" value="beta-Roll"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51125">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="520" mass="56273" checksum="32D2F9C4105C5DAD" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPW3</accession>
    <name>A0A2S2KPW3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPW3"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="78" mass="9031" checksum="E63D802EC6AE5636" modified="2018-07-18" version="1">MERMTKQNERKNKTELELLNDINLKLDKLIGVLAIQSIKDTDDKIHLLKNLDFKSDEVGPLVGIKGTSVRDREGWKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ06</accession>
    <name>A0A2S2KQ06_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7 8">Prefoldin subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">GimC subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pfdA</name>
        <name evidence="10" type="ORF">NZNM25_05200</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 7">Molecular chaperone capable of stabilizing a range of proteins. Seems to fulfill an ATP-independent, HSP70-like function in archaeal de novo protein folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7">Heterohexamer of two alpha and four beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the prefoldin alpha subunit family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876382.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ06"/>
    <dbReference type="GeneID" id="76209871"/>
    <dbReference type="OrthoDB" id="10045at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23160">
        <property type="entry name" value="Prefoldin_alpha_GimC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00308">
        <property type="entry name" value="PfdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011599">
        <property type="entry name" value="PFD_alpha_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00293">
        <property type="entry name" value="prefoldin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12674">
        <property type="entry name" value="PREFOLDIN SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12674:SF2">
        <property type="entry name" value="PREFOLDIN SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="93"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="NCBIfam" id="TIGR00293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH33729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="141" mass="15660" checksum="D120D58CD0BA3C30" modified="2018-07-18" version="1">MSEEQAEQLMQQMQMLETYFSDLSQREATFFSILREATAAIESIQSLGKNPESETLVPIGMGTYIPTKISSNSKIVLNIGAGVAVEKDFPSAINYLEARIKEIEIALQDTAAKKQDAANRLEQGRAQMNQMMQAMQQQKPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ17</accession>
    <name>A0A2S2KQ17_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative ferredoxin subunit of phenylpropionate dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_04020</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876539.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ17"/>
    <dbReference type="GeneID" id="76209748"/>
    <dbReference type="OrthoDB" id="6837at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03528">
        <property type="entry name" value="Rieske_RO_ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21496:SF23">
        <property type="entry name" value="3-PHENYLPROPIONATE_CINNAMIC ACID DIOXYGENASE FERREDOXIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21496">
        <property type="entry name" value="FERREDOXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="6" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="5">
        <location>
            <begin position="5"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="102" mass="11290" checksum="F6F86F12DF8DFFA8" modified="2018-07-18" version="1">MSEWVKACNLEQVKEGQLFGFVHGDKKLLLANLNGKIHATDLICTHADADLSTGFLSDEGVRCPLHLSVFNLDNGKPQNLPAEIPLKVYNVKIDANEIYVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ22</accession>
    <name>A0A2S2KQ22_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PurE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_05810</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876435.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ22"/>
    <dbReference type="GeneID" id="76209933"/>
    <dbReference type="OrthoDB" id="372165at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039476">
        <property type="entry name" value="P2CMN_synthase_LarB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033503">
        <property type="entry name" value="LarB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43064">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43064:SF1">
        <property type="entry name" value="SLL1489 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52255">
        <property type="entry name" value="N5-CAIR mutase (phosphoribosylaminoimidazole carboxylase, PurE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurE" evidence="1">
        <location>
            <begin position="121"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="257" mass="27578" checksum="28B3D0B12F4ACD23" modified="2018-07-18" version="1">MEINEILESLSAGKISINNAKKLLSLYSIEEVEGFAKIDINRRKRRGIPEVIFAETKELDEIKKIIKKTLEKTNSVIISRIKKNDYPEIISFSKRLKVIIKTGKNASTLLLLKKAIKSHGGKVGILTAGTSDIGVAEESRLMCEAMNCKCITGYDVGVAGIQRIFPILKKMINEEVDCIIVAAGMEGALATLVSSMVDIPVIGIPTSVGYGYGEKGIAALASMLQSCSLGLSVVNIDNGIAAGGIAANIANRAIRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ25</accession>
    <name>A0A2S2KQ25_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04950</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_160049190.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ25"/>
    <dbReference type="GeneID" id="76209847"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="53" mass="6041" checksum="C625C8E12EF20187" modified="2018-07-18" version="1">MNYYVIFSISDPNNYETIFHPNASKIGIGITMSAEKFYVLQYVCGVEDENNSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ54</accession>
    <name>A0A2S2KQ54_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0284 protein NZNM25_05930</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_05930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0284 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876444.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ54"/>
    <dbReference type="GeneID" id="76209945"/>
    <dbReference type="OrthoDB" id="9136at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008939">
        <property type="term" value="F:nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02439">
        <property type="entry name" value="DMB-PRT_CobT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01086">
        <property type="entry name" value="UPF0284"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003200">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002805">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036087">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00303">
        <property type="entry name" value="nicotinate mononucleotide-dependent phosphoribosyltransferase CobT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003372">
        <property type="entry name" value="PRK04447.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38811">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38811:SF1">
        <property type="entry name" value="UPF0284 PROTEIN SLL1500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02277">
        <property type="entry name" value="DBI_PRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52733">
        <property type="entry name" value="Nicotinate mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase (CobT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="354" mass="37771" checksum="9E8B95582EF2AF23" modified="2018-07-18" version="1">MENFELHGNIDRANNFFETMNSGRFLFSFVISYTETCEIPGITFAGADMNSIQFTPPADAEYLHYGYCKTIDKIPMTPDGKPTPGLLTKTALESASIPHLTINAGSKIVPQLPFVEAGLSFGKNISICEAMSDSQVSHAVDYGRIVGRSLASLTDCLVIGESIPGGTTTALAVLRGLGYDAKVSSSIPNNPVDLKNQIVDSALERVNSVHPYSIVAKVGDPMIPFVAGMLSSASDVSKVMLAGGTQMAAVLAFASKIGFNEENSVIGTTSYITHDESANFINLVREIADIPAISVNPNLENSRYSGLKAFSEGFAKEGVGAGGSIISSMIKTGNDSIKYLDLVEKEYHRLFTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQB8</accession>
    <name>A0A2S2KQB8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04390</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876311.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQB8"/>
    <dbReference type="GeneID" id="76209788"/>
    <dbReference type="OrthoDB" id="3038at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="96" mass="10971" checksum="57B6A0D9D63C6212" modified="2018-07-18" version="1">MALVSNTLIKILDEINNELINQISLAKNADAKIDTRLLFHHSKTLNDIIMTSVLSNENKLMLQKFSSTLVQDNTTVKSLRYEKQDLERVLSNLKDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQD0</accession>
    <name>A0A2S2KQD0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription initiation factor IIB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tfb_4</name>
        <name evidence="9" type="ORF">NZNM25_06500</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TFIIB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQD0"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20550">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000023">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="8">
        <location>
            <begin position="12"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="304" mass="33919" checksum="6346E54873B8359C" modified="2018-07-18" version="1">MQSIFDFIMSLQETKCPRCGKNTVITDVDSQEVFCSKCGVVLDEKVNDSRSERIFTNSTVNRSHTGDKTTLTRHDRGLSTMINPFDKDSSGNPLSASMKSSLKRLRKWDNRSHMKNNDERNLQQALSELLKLKEKLSLPDAVVEKASYIYRKALEKKLVRGRSIAALIAAALYAACRESETPRTLQEIASSMGIKRKELTASYRLIFKELELKIPVADSVSCIAKIASNAELSEKTKRHAMKILKKAEKQNALAGKHPMGLAASALYLASIDLEENRTQKDIADAAGITEVTIRNRCKGLRTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQE0</accession>
    <name>A0A2S2KQE0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Large ribosomal subunit protein uL1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">rpl1</name>
        <name evidence="13" type="ORF">NZNM25_05320</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Binds directly to 23S rRNA. Probably involved in E site tRNA release.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Probably involved in E site tRNA release. Binds directly to 23S rRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Protein L1 is also a translational repressor protein, it controls the translation of its operon by binding to its mRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 11">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11 12">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQE0"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.790:FF:000005">
        <property type="entry name" value="50S ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01318_A">
        <property type="entry name" value="Ribosomal_uL1_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_uL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023669">
        <property type="entry name" value="Ribosomal_uL1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023673">
        <property type="entry name" value="Ribosomal_uL1_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003244">
        <property type="entry name" value="PRK04203.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL1M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01199">
        <property type="entry name" value="RIBOSOMAL_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0678">Repressor</keyword>
    <keyword evidence="9 11 12" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8 11 12" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="7 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 11" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="4 11" id="KW-0820">tRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH33741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="223" mass="24704" checksum="68425F5214A529C7" modified="2018-07-18" version="1">MFVMITETQLADMIKQAKAATKPKKFTQSIELIANFKDIDVKKGFALNEVVQLPKTTSPATVCIMATGEMGQKAKQANADSVIGTEELDKFASNKRESRKFINKYDFFLADTKVMPTVGKTLGQLLGPRGKMPTPVPFDAPIDAFLSRFRSSIKVRTRASLSVSCKIGDETMDDGDLAINAHTVLNAIEKKLPNGEKNMKRIMIKTTMGKPVKQIQEVRKKYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQG2</accession>
    <name>A0A2S2KQG2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876536.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQG2"/>
    <dbReference type="GeneID" id="76210063"/>
    <dbReference type="OrthoDB" id="381931at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="64" mass="7801" checksum="B33D558021230434" modified="2018-07-18" version="1">MKNNKIKDLYSKIFLYVRGVMEQKEFHLMVDMLKTAIKQEREFTKDELKKFDDLFEKIIDVKVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQI4</accession>
    <name>A0A2S2KQI4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0147 protein NZNM25_06080</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_06080</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0147 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876458.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQI4"/>
    <dbReference type="GeneID" id="76209961"/>
    <dbReference type="OrthoDB" id="65304at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.50">
        <property type="entry name" value="Ta0600-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00342">
        <property type="entry name" value="UPF0147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023130">
        <property type="entry name" value="Ta0600-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005354">
        <property type="entry name" value="UPF0147"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003319">
        <property type="entry name" value="PRK04330.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03685">
        <property type="entry name" value="UPF0147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158436">
        <property type="entry name" value="Ta0600-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="89" mass="9971" checksum="7C61A460782428D7" modified="2018-07-18" version="1">MADNAQNKESMKEAIDTLNQIVSSNSTPKTIKKSITDLIVDLNNEEYSLSVRAANTISLLDDVTQDPNMPSYVRTQLWQAVSKLESIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQK4</accession>
    <name>A0A2S2KQK4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">4Fe-4S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_06850</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876523.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQK4"/>
    <dbReference type="GeneID" id="76210046"/>
    <dbReference type="OrthoDB" id="23833at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050572">
        <property type="entry name" value="Fe-S_Ferredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687">
        <property type="entry name" value="ADENYLYLSULFATE REDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687:SF6">
        <property type="entry name" value="L-ASPARTATE SEMIALDEHYDE SULFURTRANSFERASE IRON-SULFUR SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="68"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="128"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="169" mass="18326" checksum="08AE19729EB6B2FA" modified="2018-07-18" version="1">MPIAENFPEGLKPLGKIKLDDGNFHIMWGPGKEKNTDGSQAEVFADADVVAAYAARGEEQVPLGVSGTMVAVDWDSCVADGACIEACPVQVFQWYRTEKDIPAKDVVGQTFNGTGSNVKDERKDLTDKADPIREHDCIWCMACVSVCPPQAIKVDQSNVEKHESAAKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQN2</accession>
    <name>A0A2S2KQN2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_06580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876497.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQN2"/>
    <dbReference type="GeneID" id="76210015"/>
    <dbReference type="OrthoDB" id="2830at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041213">
        <property type="entry name" value="NitrOD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052048">
        <property type="entry name" value="ST_Response_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228:SF1">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ARR22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18549">
        <property type="entry name" value="NitrOD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="1">
        <location>
            <begin position="3"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="200" mass="22569" checksum="1CDF8B2749ED0838" modified="2018-07-18" version="1">MVSCIVIDDDQNTVDLFCELLSMIKVDVLARGYDGRDAVKLYEKHVPDIVFTDLQMPAYDGLYAVENIKDKYPDAKIIMVTGDINATESELLESLNVHVIDKPFDIHTIKQTVTDVLLDEDSKSSIKIQYMFKEDDSVYSCVVTYEQYKNLKILPIIQECKIIGPGQNSLESYTASMQKALDLACKNNTTNIRKLSEIVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQQ5</accession>
    <name>A0A2S2KQQ5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thrombospondin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_07930</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866842.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQQ5"/>
    <dbReference type="GeneID" id="76208883"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1080.10">
        <property type="entry name" value="TSP type-3 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003367">
        <property type="entry name" value="Thrombospondin_3-like_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028974">
        <property type="entry name" value="TSP_type-3_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10199">
        <property type="entry name" value="THROMBOSPONDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10199:SF100">
        <property type="entry name" value="THROMBOSPONDIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02412">
        <property type="entry name" value="TSP_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103647">
        <property type="entry name" value="TSP type-3 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="488" mass="55232" checksum="2A8AF00B02219F9A" modified="2018-07-18" version="1">MAIFVIDKKIKFLFVLLIISLGVSFLAWSEYAIFADSDNDGTSDSFDNCPLNPNMDQSDFDLDKSGDVCDTDDDNDGVKDNLDQFDTDPLEWADFDFDNLGANQDSDDDNDGLTDMEDSFPILVSQKLVEENLSEIESCAILETGTSKLLCYSQFFQSLVVKEENNVDTLELALSLTQLGAVDDCHFISHEIGHAAYAENSNIFENLSGVDGSVCRGGFYHGVMAAYFHELQENNKDMGEYKTICNDFIGKPEYTKCVHGLGHGITHYFINDLNSAINACDQMSFYQSSICVGGVFMQYTDDELTRSTSIKQDIQNICPKSDLRIFDYQQCRDNLGLSIAFHTDHDLEEGSKLCDMIIDDMGKQYCHRGLEREINDAKEYKVYDPTKGVRELMQPVWIKENDSNKWIVDFRSPSKISNVVYDETTKMMQFSFDAPYRIIIYMSTDLLPENPVVMINGQQNDFEIQHGLYDNHSMIQIMPKNSGVVLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQS1</accession>
    <name>A0A2S2KQS1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_07550</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQS1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="PIN" evidence="2">
        <location>
            <begin position="74"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="377" mass="42867" checksum="0E2720E5FEF53F8A" modified="2018-07-18" version="1">MLMFVKYYLRRKIKCSYDVSNILNIDEHISKVETFLDNATKGLDSDTLLWLKQQFEENMGNFIENCSGGLKVTMVLDANIAISTIQRYDSNKNSILHHLKDNPMFTIIAPPLIEEEVIKFIDSPEQAKSRTKLRKEWKRLKKTIQIKEVTDKRILEAALKITSRRDPKDSQYVALYIETNSNAILTKDDDFNDFSVRKFDITNLDKVVGTFHRGVYSFFIFHDAFPLVFELSGKIIAAILKNAISFLALILSLVTNAVTKSFEKLIDLFSKMSKGTQQAIGALLLIGSIVSIIILVLREDVREKASKKLSSLTKKAKEIIEKILEWITRSFEILRTYAGKAAPYGITSLEIIAEIQEHIDAISDELKNISTDPASYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQS2</accession>
    <name>A0A2S2KQS2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CbtB-domain containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_07970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQS2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012667">
        <property type="entry name" value="CbtB_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09489">
        <property type="entry name" value="CbtB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="72" mass="8065" checksum="A73DF139CA23EB20" modified="2018-07-18" version="1">MLSLPTEIMVSKSKVPVFAIMLLAMIFVVGLFVVGYDQGHIFSVVLGEQAYEDLYIHELTHDMRHAAGFPCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR28</accession>
    <name>A0A2S2KR28_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cheY-4</name>
        <name evidence="3" type="ORF">NZNM25_09070</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876744.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR28"/>
    <dbReference type="GeneID" id="76208763"/>
    <dbReference type="OrthoDB" id="2830at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050595">
        <property type="entry name" value="Bact_response_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591:SF3">
        <property type="entry name" value="RESPONSE REGULATORY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591">
        <property type="entry name" value="STRESS RESPONSE REGULATOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="6"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="129" mass="14260" checksum="83A21E7C61650788" modified="2018-07-18" version="1">MGINPRVVVIDDSKSNLDVFCEFLTLNNVDVVGQGKNGVEAVQLFEQHDPDVMLVDLAMPVYDGYFALNNILAVDKNAKVVIITALEGKKDTPKLLKLGAVDVLQKPIDLNRVVDVINNIFKEKKLLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR46</accession>
    <name>A0A2S2KR46_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histidine kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_08020</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR46"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065:SF10">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065">
        <property type="entry name" value="SENSOR HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="304"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="348"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="547" mass="61487" checksum="A6BFA508CE04E77F" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR55</accession>
    <name>A0A2S2KR55_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08380</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876684.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR55"/>
    <dbReference type="GeneID" id="76208834"/>
    <dbReference type="OrthoDB" id="382440at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="231" mass="25855" checksum="16613D3E21D9D908" modified="2018-07-18" version="1">MKTRYKIILVAVCAYFGVFFGPVITSNVYCDFVSQEMCTSRITGVVLPPLNLIMPSLPKDNCVVDNDGVMEPCYNDAGLLEWPFPPRMEVHTERECNKICADVEKLPPCTSDRTACFNQDAHLCDPSGWECGDNEGIFEEIIEYNSDATSYDLQMMFDEKESAVEYFVVNGNTDHVEIDIPTDLIDGVFMIHVNGENIDDKRVSIDGNKVIVNYGQNIESVKLFGSYDLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR89</accession>
    <name>A0A2S2KR89_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Roadblock/LC7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08680</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR89"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="132" mass="15072" checksum="BDE3A03DAB6A4CFE" modified="2018-07-18" version="1">MTCDITNVDFQNICKSISALEHDIYFVGLINDNGRLVEYVKKQNILIDDLEKDGLIMLCMQTRLYASMQSDHNKHFGRFGYSIIEREKTTLLTIPTVYGTVLVITSNQVDSKTLAKQISQVAYRDCFAKILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRE0</accession>
    <name>A0A2S2KRE0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_09020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876740.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRE0"/>
    <dbReference type="GeneID" id="76208767"/>
    <dbReference type="OrthoDB" id="142096at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054162">
        <property type="entry name" value="DUF6293_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046260">
        <property type="entry name" value="HFX_2341-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19810">
        <property type="entry name" value="HFX_2341_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22665">
        <property type="entry name" value="WHD_DUF6293"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HFX-2341-like N-terminal" evidence="1">
        <location>
            <begin position="8"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6293" evidence="2">
        <location>
            <begin position="150"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="247" mass="28775" checksum="E62A6A08E247C199" modified="2018-07-18" version="1">MSKHAQLRIHIAPLGFESDRIFLPAIEMKADKVWILIHNNPKKTKAKSYLDDIKKRLKKERIECVTKELDRLDLFSNIKTIRNIVDDEGEDNTYYINVSSGSKIQAVACTMACMMFNKKNDLVPYYAQPENYFEYDGEQISTGLDRIIDMPQYDIKTPDKKFIQTLRIIKENKNRIDKTNLAIAAEKENIITVGSEENFEQARFTSLNKNIIQPLKNDWGFIREEKIGRTRWVEITPEGKNVLEILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRE5</accession>
    <name>A0A2S2KRE5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_10270</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876847.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRE5"/>
    <dbReference type="GeneID" id="76208638"/>
    <dbReference type="OrthoDB" id="2219at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13671">
        <property type="entry name" value="AAA_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="15598" checksum="B21160711CCA0120" modified="2018-07-18" version="1">MIGVALSGKTTYIKANFDHKRISLSFFDNNRKKELEYIEECLNRGKSIVIDDTNLTEDIRKRHIDLAKKYNAKVRGVFMNTSRGLLEKRQKSRRDPFPLSAIYKQLNELETPVMGEGFDELIIKKDYERPRET</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRF4</accession>
    <name>A0A2S2KRF4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Roadblock/LC7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876667.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRF4"/>
    <dbReference type="GeneID" id="76208854"/>
    <dbReference type="OrthoDB" id="8852at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103196">
        <property type="entry name" value="Roadblock/LC7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="12933" checksum="DC80AF2F3E22B409" modified="2018-07-18" version="1">MEIEAAVEKLLKLSPSVRVVTICDLKGKLVYSARSKKVSILVSKKQSLASLKAAAYDWNQRKKLLRTLGACKYVVAEYDKVKRIVIPAGKNHILYVTTTAAFDHNKVVRKVRSFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRF6</accession>
    <name>A0A2S2KRF6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">L-glutamate gamma-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="2">1.2.1.88</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">putC</name>
        <name evidence="7" type="ORF">NZNM25_10200</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-glutamate 5-semialdehyde + NAD(+) + H2O = L-glutamate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:30235"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="EC" id="1.2.1.88"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.88" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876840.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRF6"/>
    <dbReference type="GeneID" id="76208647"/>
    <dbReference type="OrthoDB" id="6342at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003842">
        <property type="term" value="F:L-glutamate gamma-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:L-proline catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000005">
        <property type="entry name" value="1-pyrroline-5-carboxylate dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050485">
        <property type="entry name" value="Proline_metab_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42862">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42862:SF1">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE 2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="6">
        <location>
            <begin position="56"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="523" mass="57501" checksum="5EB03AF728E4181A" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRF7</accession>
    <name>A0A2S2KRF7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08260</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876673.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRF7"/>
    <dbReference type="GeneID" id="76208847"/>
    <dbReference type="OrthoDB" id="3099at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="77" mass="9154" checksum="E014F2CC3476D9C6" modified="2018-07-18" version="1">MNEITDEDRERVKLLQQITSSKNEFKKLSLEQLQRLQELIEKKDYSHDKKAHKSKVKLLGKINVRIYELTEGRGIWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRG6</accession>
    <name>A0A2S2KRG6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_10470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876863.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRG6"/>
    <dbReference type="GeneID" id="76208617"/>
    <dbReference type="OrthoDB" id="11839at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF205">
        <property type="entry name" value="ZINC TRANSPORTER ZUPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="309"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="340"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="373"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="392" mass="42196" checksum="5DA391497B5FBEE0" modified="2018-07-18" version="1">MELDEKKSHAKVIASGIIPFAFIILMMAYIFGPGSDLLDLGIPLPEITIEKVDFVDSEIQATVRNTGPIPVEVAMADVNDRIQPAAVEPDRFLERYEVVLVRIPFEWNEAEPYIIGITVEDGTRFEKKIDAAAPALEPTVELAVFFAIIGTYVGIIPVMIGLLWLPFIKKIKKSKYHFFLALTAGLLLFLGIDSIEEAIQVSDESLADSFNGTLLVATITVLSFLGLYYTGEKLVKRSETSRLTKPVAIALMIAIGIGLHNFGEGLAIGAAVGLGSIAFSTFLIVGFALHNTTEGIAIASPMSREKTVIWKLAAMGMIAGSPAIFGAWVGGFVYSPFSSVIFLSIGAGAIFQVIITILKWIRVEGDKNLSSAAVASGFAIGMLIMYATSILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRJ8</accession>
    <name>A0A2S2KRJ8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dihydroorotate dehydrogenase</fullName>
            <shortName evidence="9">DHOD</shortName>
            <shortName evidence="9">DHODase</shortName>
            <shortName evidence="9">DHOdehase</shortName>
            <ecNumber evidence="9">1.3.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pyrD</name>
        <name evidence="11" type="ORF">NZNM25_10640</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the conversion of dihydroorotate to orotate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(S)-dihydroorotate + A = orotate + AH2</text>
            <dbReference type="Rhea" id="RHEA:18073"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="9">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Pyrimidine metabolism; UMP biosynthesis via de novo pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.-.-" evidence="9"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876875.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRJ8"/>
    <dbReference type="GeneID" id="76208597"/>
    <dbReference type="OrthoDB" id="36608at2157"/>
    <dbReference type="UniPathway" id="UPA00070"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004152">
        <property type="term" value="F:dihydroorotate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04740">
        <property type="entry name" value="DHOD_1B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000027">
        <property type="entry name" value="Dihydropyrimidine dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00224">
        <property type="entry name" value="DHO_dh_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050074">
        <property type="entry name" value="DHO_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033888">
        <property type="entry name" value="DHOD_1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024920">
        <property type="entry name" value="Dihydroorotate_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012135">
        <property type="entry name" value="Dihydroorotate_DH_1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049622">
        <property type="entry name" value="Dihydroorotate_DH_I"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005574">
        <property type="entry name" value="PRK07259.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01037">
        <property type="entry name" value="pyrD_sub1_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109:SF1">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (FUMARATE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000164">
        <property type="entry name" value="DHO_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00912">
        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 9" id="KW-0288">FMN</keyword>
    <keyword evidence="8 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroorotate dehydrogenase catalytic" evidence="10">
        <location>
            <begin position="5"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="46"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="71"/>
            <end position="75"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="190"/>
            <end position="191"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="241"/>
            <end position="242"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="263"/>
            <end position="264"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="303" mass="32430" checksum="A713B9F3411DD95E" modified="2018-07-18" version="1">MELSLATSIGQIQLDRPVMLASGILGISLDVFNRLYRSGVGAVVTKSLSTEPWEGYPNPTIFSVKGGGWINAVGLSNPGATNFAKMIEPNQDVPIVVSLVGSIPEDFEMMIKQFENCKVTAYELNLSCPHVAKVGLEVGDDPELVKKIVTTAKNTTDVPIIAKVGLGTTHYLNTVSAAIDSGIDAITAINTVRAMAIDVETQRPILSNKFGGLSGTPIKPIALRCVYEISSKHDIPIIGCGGISTWEDAVEFFLAGASAIQLGSAVGDNWIDVFNDINNGILQYMKRKNYSSIKEMVGLAKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRN1</accession>
    <name>A0A2S2KRN1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Helicase HerA central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_08990</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 8">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HerA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876737.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRN1"/>
    <dbReference type="GeneID" id="76208770"/>
    <dbReference type="OrthoDB" id="107033at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008571">
        <property type="entry name" value="HerA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957:SF1">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase HerA central" evidence="5">
        <location>
            <begin position="145"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="527" mass="58792" checksum="6C86F52EDB965F0D" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRQ7</accession>
    <name>A0A2S2KRQ7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Aspartate ammonia-lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ansB</name>
        <name evidence="4" type="ORF">NZNM25_09190</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876754.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRQ7"/>
    <dbReference type="GeneID" id="76208751"/>
    <dbReference type="OrthoDB" id="26319at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.10:FF:000001">
        <property type="entry name" value="Fumarate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000001">
        <property type="entry name" value="Fumarate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051546">
        <property type="entry name" value="Aspartate_Ammonia-Lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018951">
        <property type="entry name" value="Fumarase_C_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008909">
        <property type="entry name" value="PRK12273.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42696">
        <property type="entry name" value="ASPARTATE AMMONIA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42696:SF2">
        <property type="entry name" value="ASPARTATE AMMONIA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10415">
        <property type="entry name" value="FumaraseC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarate lyase N-terminal" evidence="2">
        <location>
            <begin position="11"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarase C C-terminal" evidence="3">
        <location>
            <begin position="406"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="453" mass="49047" checksum="C61D9A1187B49DCC" modified="2018-07-18" version="1">MKFRLDQDSLGKIKIPSDAYYGAFTGRAIKQYHVTGNKSHENLIKSFVMIKRSAAVANMKTKAIDAKRGKAIVSACDKIISGKYIDQFVVDMINSGAGTAFNMNSNEVIANVALEVLHKKKGQYEFLHPNDHVNMSQSSNDTYPTAMHVAILMNLKDTIPAIDVLIKSLSKKAKQFSSFKKIGRTHLMDALPVTLGSEFGAYVTSITKARNEIVSSQKELQNVALGGTAVGSGANTPKGYRKIAISELSKISKLALKPERDMQHGLQSKFAVANASSALRNLALEIGKLANDIRLMASGPIAGLAELGIPAVHAGSSIMPGKVNPSLAECMNMICFNIIGNDTAVSYAAQSGQFELNVMLPGMLKCMLESTDMLKNFLPIFSANLIDGLTANKEKLRADIENSPVIVTLLTPKIGYLKSAELFKESLKTGKTIRELVVSKKLMSNKEIDSLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRS8</accession>
    <name>A0A2S2KRS8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Putative [LysW]-L-2-aminoadipate/[LysW]-L-glutamate phosphate reductase</fullName>
            <ecNumber evidence="7">1.2.1.103</ecNumber>
            <ecNumber evidence="7">1.2.1.106</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">lysY</name>
        <name evidence="9" type="ORF">NZNM25_09390</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in both the arginine and lysine biosynthetic pathways.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[amino-group carrier protein]-C-terminal-N-(1-carboxy-5-oxopentan-1-yl)-L-glutamine + phosphate + NADP(+) = [amino-group carrier protein]-C-terminal-N-(1-carboxy-5-phosphooxy-5-oxopentan-1-yl)-L-glutamine + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9712"/>
            <dbReference type="Rhea" id="RHEA-COMP:9714"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78499"/>
            <dbReference type="ChEBI" id="CHEBI:78501"/>
            <dbReference type="EC" id="1.2.1.103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[amino-group carrier protein]-C-terminal-gamma-(L-glutamyl-5-semialdehyde)-L-glutamate + phosphate + NADP(+) = [amino-group carrier protein]-C-terminal-gamma-(5-phospho-L-glutamyl)-L-glutamate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52668"/>
            <dbReference type="Rhea" id="RHEA-COMP:13313"/>
            <dbReference type="Rhea" id="RHEA-COMP:13327"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:136717"/>
            <dbReference type="ChEBI" id="CHEBI:136761"/>
            <dbReference type="EC" id="1.2.1.106"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-arginine biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (Thermus route): step 3/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NAGSA dehydrogenase family. Type 1 subfamily. LysY sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.103" evidence="7"/>
    <dbReference type="EC" id="1.2.1.106" evidence="7"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876773.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRS8"/>
    <dbReference type="GeneID" id="76208731"/>
    <dbReference type="OrthoDB" id="372053at2157"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00037"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043870">
        <property type="term" value="F:N-acetyl-gamma-aminoadipyl-phosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003942">
        <property type="term" value="F:N-acetyl-gamma-glutamyl-phosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070401">
        <property type="term" value="F:NADP+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23939">
        <property type="entry name" value="AGPR_1_C_LysY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17895">
        <property type="entry name" value="AGPR_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00150">
        <property type="entry name" value="ArgC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02083">
        <property type="entry name" value="LysY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050085">
        <property type="entry name" value="AGPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058924">
        <property type="entry name" value="AGPR_dimerisation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000706">
        <property type="entry name" value="AGPR_type-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037535">
        <property type="entry name" value="LysY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01850">
        <property type="entry name" value="argC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32338:SF11">
        <property type="entry name" value="[LYSW]-L-2-AMINOADIPATE_[LYSW]-L-GLUTAMATE PHOSPHATE REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32338">
        <property type="entry name" value="N-ACETYL-GAMMA-GLUTAMYL-PHOSPHATE REDUCTASE, CHLOROPLASTIC-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22698">
        <property type="entry name" value="Semialdhyde_dhC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 7" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0521">NADP</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Semialdehyde dehydrogenase NAD-binding" evidence="8">
        <location>
            <begin position="2"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="9"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="348" mass="38650" checksum="11F91B3CA4D61B7B" modified="2018-07-18" version="1">MKVGVVGASGYVGGETLRLLVNHPDVEIAMVTSRQHVGEYLHRVQPSLKGFTDLMFSELDYDKLTDQCDLVFTAVPHGTATEIVKALYDRGIKVIDLSADYRLHNQDAYDKWYGWEHPHPDYLAKSVFGVPELHREAIKKAQLVSCPGCMAVTSMLALAPLIRNDIIDTEHIVVDSKIGSSGAGSGSGTAHAMRAGVIRPYKPAKHRHTGEIEQELSEIAGHKIRVSMSPHAVDVVRGILCTNHTFLKKDIEEKELWKLYRQAYGEERFIRLIRDKKGLYKFPDPKFLVGSNFCDIGFDLDEDNNRLIALSASDNLMKGAAGSAIQNMNVMCGFDEMDGLRNTPLTPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRY8</accession>
    <name>A0A2S2KRY8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Recombinase RecJ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_11650</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877001.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRY8"/>
    <dbReference type="GeneID" id="76208487"/>
    <dbReference type="OrthoDB" id="36101at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1640.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048515">
        <property type="entry name" value="DHH_CID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051673">
        <property type="entry name" value="SSDNA_exonuclease_RecJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30255">
        <property type="entry name" value="SINGLE-STRANDED-DNA-SPECIFIC EXONUCLEASE RECJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30255:SF2">
        <property type="entry name" value="SINGLE-STRANDED-DNA-SPECIFIC EXONUCLEASE RECJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21763">
        <property type="entry name" value="DHH_CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDH" evidence="1">
        <location>
            <begin position="26"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="DHH-CID" evidence="3">
        <location>
            <begin position="197"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="2">
        <location>
            <begin position="389"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="473" mass="52214" checksum="7089AA972A0F61CE" modified="2018-07-18" version="1">MTKSLDESLSYFKDKISDCIKSKKSIFVTTHIDCDGLTSGSIIAKALIREGANCTVRTSKEFSKNVVESFKKDSRDFHVVTDLGGGFANELDSTLGDNWIVLDHHQIPDKEIDNQNVINAWKYGIDGGVEICAGGMAYLASIALDEKNSDLSAIAVVSALGDRQDQGERKSFTGKNFEISNTAKELGLVDIDLDLLLVGRETRPLPDALAFTSQPFIEGLTWNRDACLSLLNSSGIKLKEGGRWRVPAELNEEEKRQVIESITKFTSGKNATEIMSELIGYTYTFPREDNRSFLRDGREFSTMLNSCGRINRSGVGMAICMGDRNKILREGESILTDYRKMIREYMSILSNERWRISESETCVMVNGEDIVPETMTGTISSLIAGSPKNSGKIVILRTKGEENTIKFSSRKSFSCNSDINLSEIMRTGAEKFDGIGGGHNAAAGAKITKDKLDEFLNYLEVNVVNMPSKSSTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS23</accession>
    <name>A0A2S2KS23_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12630</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877101.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS23"/>
    <dbReference type="GeneID" id="76209239"/>
    <dbReference type="OrthoDB" id="4303at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="157" mass="18503" checksum="E75EB80AA9713BBA" modified="2018-07-18" version="1">MSYEINWHKNFYGISWAYEKDRLVVSTVFEDKKEAISIAKEVEDWSDKFTRLTIIEKDDDEFAICCYQDPQISKSATHVGLFRTGMRQSGGYEQAKPMIEEKSPLLQIAYAADVRDMNTYEQISRLVPMRKCRIISEKDLKKQEYYYEKNASIQNQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS83</accession>
    <name>A0A2S2KS83_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Roadblock/LAMTOR2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12650</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866884.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS83"/>
    <dbReference type="GeneID" id="76209237"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="130" mass="14396" checksum="E9FC10D4F8854182" modified="2018-07-18" version="1">MNFENLLQRIMDSDINIRHSIITDIEGNILATSHRDGITNYLSPEETAASLKRAATAWKGRKALSPKIGDGLYAVAAFEKITRMTFPLGNENLIFVSMGSDSVRTDMHAGGQQQIVQHVLNILSRDPTKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS88</accession>
    <name>A0A2S2KS88_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Agmatinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">speB</name>
        <name evidence="6" type="ORF">NZNM25_13040</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="4">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the arginase family. Agmatinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877130.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS88"/>
    <dbReference type="GeneID" id="76209195"/>
    <dbReference type="OrthoDB" id="7186at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033389">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via agmatine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11593">
        <property type="entry name" value="Agmatinase-like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005925">
        <property type="entry name" value="Agmatinase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01230">
        <property type="entry name" value="agmatinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="ARGINASE/AGMATINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358:SF26">
        <property type="entry name" value="GUANIDINO ACID HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036979">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR036979-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="290" mass="32388" checksum="642D89FE2A4F7941" modified="2018-07-18" version="1">MSFFDLYMNRNPLITSSDDDTEPVATVFGVPFDSTHSYKPGCRFGPDAIRDSFNNIEIFHPDLGVNLETVNIEDLGNTRHTVVASEMIDMVKKITTELVAKQRQLFILGGEHSITYGTYTSFPKETGYIVFDAHYDLRDEFADIKLSHASYLRRVVEERGSENILHVGARAFVKEELEFLKENNIKTISDREIRDGKGPQLLKDHVSTFDTIYSSFDLDVLDPAFAPGVGNPEAVGITSRELFDMIHSFSETKVIGVDVVELNPYHDNGSTASLAAKIISTLIAMNLSPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSE3</accession>
    <name>A0A2S2KSE3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">dCMP deaminase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_13500</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSE3"/>
    <dbReference type="OrthoDB" id="7284at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004132">
        <property type="term" value="F:dCMP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006220">
        <property type="term" value="P:pyrimidine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01286">
        <property type="entry name" value="deoxycytidylate_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016473">
        <property type="entry name" value="dCMP_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015517">
        <property type="entry name" value="dCMP_deaminase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035105">
        <property type="entry name" value="Deoxycytidylate_deaminase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11086:SF18">
        <property type="entry name" value="DEOXYCYTIDYLATE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11086">
        <property type="entry name" value="DEOXYCYTIDYLATE DEAMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006019">
        <property type="entry name" value="dCMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="3">
        <location>
            <begin position="9"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="175" mass="19518" checksum="C55E2A6E93982BEE" modified="2018-07-18" version="1">MNNTFDRPGWDEYFMLQAELAKLRSNCMTRQVGAVIVRNHRQIATGYNGTPPGIKNCFEGGCKRCTLRMEGKIESGASLDRCLCNHAEANAIMHCAILGIEAGIEGAVLYTTFVPCLECTKMAITIGIRKFVCLDSYPETDYDLLKEAGVEVIQLEKNKIVKWASQLVNKYEKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSG0</accession>
    <name>A0A2S2KSG0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12980</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877125.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSG0"/>
    <dbReference type="GeneID" id="76209201"/>
    <dbReference type="OrthoDB" id="3123at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="69" mass="7964" checksum="F31C7FD37382C1F2" modified="2018-07-18" version="1">MDISSKKLPMILIVILLGILIVQFVSNDSDKKFIDVETCEIWVEDSLTKKPRYLGEYDSKCLDFKNLNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSH9</accession>
    <name>A0A2S2KSH9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Roadblock/LAMTOR2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877055.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSH9"/>
    <dbReference type="GeneID" id="76209299"/>
    <dbReference type="OrthoDB" id="7817at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="128" mass="14580" checksum="21CF1A433E9AEE15" modified="2018-07-18" version="1">MAKTPADKWKDAIVKYRKQCQNILKLSDSIRYAGVINAYGRTLTGIIKPDLKPLLKSEQVKNEFFIISTLMSLRKETIGTVGILEHVILQHQNVTIVILQKKDVTFYVSIDRKEKGIEPIISSIKKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSI3</accession>
    <name>A0A2S2KSI3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator AsnC/Lrp ligand binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_12090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877058.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSI3"/>
    <dbReference type="GeneID" id="76209296"/>
    <dbReference type="OrthoDB" id="9416at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="1">
        <location>
            <begin position="15"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="72" mass="7897" checksum="FB345A3A708CD498" modified="2018-07-18" version="1">MVRAIILVKSPKKLIAARLRKISSVSDSFPTSGQFDAVAIIDVKQLSQIKDVATLIQKISGVERTETMVEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSK2</accession>
    <name>A0A2S2KSK2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_14270</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSK2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001080">
        <property type="entry name" value="3Fe4S_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43948">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43948:SF10">
        <property type="entry name" value="MRJ, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13370">
        <property type="entry name" value="Fer4_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00352">
        <property type="entry name" value="3FE4SFRDOXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="18"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="164"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="71"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU368020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="238" mass="27602" checksum="CE9C3CEF726219BD" modified="2018-07-18" version="1">MQLFLSYDKSLSSVNTYQAIKVLNVNQDSSQDEIKAAYRKLALEWHPDKNKNAVSDTEFKKITEAYNFLKKNHNHQNSSSQKKHAEKPNTKTRYKKKPQWGAPDDGGIPEQDWSKYTREFEEGDPDFWKEYERKFWEEYNARVRPDGRNGEYEKAQEPKKQPNLFVDVDKSRCIGCCSCEMIAPDVFEINKESRSNPKSSVINQKGAGVNKIMNAAETCPTKAIIVENIDSNERLYPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSL2</accession>
    <name>A0A2S2KSL2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxamine 5'-phosphate oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_13380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877158.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSL2"/>
    <dbReference type="GeneID" id="76209160"/>
    <dbReference type="OrthoDB" id="4669at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="PNPOx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyridoxamine 5'-phosphate oxidase N-terminal" evidence="1">
        <location>
            <begin position="11"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="132" mass="15268" checksum="D8F658CD9FE0C849" modified="2018-07-18" version="1">MIEFSKNEIVFLESLEEARIATSHNDIPHVKPVSYVLKDNVILVATDYNTRTFSNIKSNPNTGIVVDIYKSGNHKAVCIQGKTQIIESGPEFKKYYDVFYKKFEWVRRDPWTENEAPFLKIIPSNKVSWGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSL3</accession>
    <name>A0A2S2KSL3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HPt domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_14610</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877262.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSL3"/>
    <dbReference type="GeneID" id="76209034"/>
    <dbReference type="OrthoDB" id="11252at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="entry name" value="HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008207">
        <property type="entry name" value="Sig_transdc_His_kin_Hpt_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="Histidine-containing phosphotransfer domain, HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50894">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HPt" evidence="2">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphohistidine" evidence="1">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="119" mass="13158" checksum="4765F86CAAB11F4C" modified="2018-07-18" version="1">MSDEFIKIATAEINEEISAISSILNSCTANEDVFQNSKELQSHTHKIKGLAPMMGQEELGSICSMLDAVLKQINDGKKIEGIYDIFTESLPFMKNSMSEPGYDMTSIIDKVTNFSSNVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSP1</accession>
    <name>A0A2S2KSP1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Schlafen AlbA-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_14350</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877240.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSP1"/>
    <dbReference type="GeneID" id="76209060"/>
    <dbReference type="OrthoDB" id="114576at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.30">
        <property type="entry name" value="Schlafen, AAA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007421">
        <property type="entry name" value="Schlafen_AlbA_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038461">
        <property type="entry name" value="Schlafen_AlbA_2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30595">
        <property type="entry name" value="GLPR-RELATED TRANSCRIPTIONAL REPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30595:SF6">
        <property type="entry name" value="SCHLAFEN ALBA-2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04326">
        <property type="entry name" value="SLFN_AlbA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Schlafen AlbA-2" evidence="2">
        <location>
            <begin position="26"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="321" mass="37123" checksum="C8C37005DF483242" modified="2018-07-18" version="1">MIEIPDDVEQWTYGKIVDLVNEGYDENDILEIKQDVNEDSDRFARSVCAFANTNGGTIIFGIDNNRKKPLHLNDRICGLDDSDQLKRNIIDKIKHIQPSIPIKNLIFRKTNIKLPNKRVIVILKVTPSSRKPHEWNHVFLKRLSDGNEPMDVTEVKQTILESQKNERLLTLLRQEAGLLRYLFEKSKKLLEEDKIRLTTMFLNTIPVDTVKYFMLNQSFLYPVQSTQSLMRILEVVEKMESISKKHSTTNILNGDLKKSLENHIALGLQILIPQGLKHLSELENHLDVKLIPTIETEEYDPLFDKLENPNDNLNDQTSNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSQ8</accession>
    <name>A0A2S2KSQ8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_13720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877186.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSQ8"/>
    <dbReference type="GeneID" id="76209127"/>
    <dbReference type="OrthoDB" id="380138at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="137" mass="15977" checksum="6FE2F505864DEBA4" modified="2018-07-18" version="1">MYNYQNQNKTKKQKTCVGIEYEVERPKLYTMKKVALFGMFSLLSILLLNVPIMLENTFAYGIDGHENPEYIDKESLVKIHFVTGSPVTSNQITDYYDSNSSEILEERTNQRNHFSYEQTGISYDSSSTDNIHEKRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSR7</accession>
    <name>A0A2S2KSR7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pyridoxamine 5'-phosphate oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_14970</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877293.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSR7"/>
    <dbReference type="GeneID" id="76208998"/>
    <dbReference type="OrthoDB" id="6613at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070967">
        <property type="term" value="F:coenzyme F420 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052019">
        <property type="entry name" value="F420H2_bilvrd_red/Heme_oxyg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019965">
        <property type="entry name" value="PPOX_F420-dep_Rv2061_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03666">
        <property type="entry name" value="Rv2061_F420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35176">
        <property type="entry name" value="HEME OXYGENASE HI_0854-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35176:SF11">
        <property type="entry name" value="PYRIDOXAMINE 5'-PHOSPHATE OXIDASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="PNPOx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyridoxamine 5'-phosphate oxidase N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="122" mass="13914" checksum="EE380588400D08CB" modified="2018-07-18" version="1">MISLEKIKSEKYIVLETYRKNGEPVKTPVWFVINDDFVYVVTRSNTGKIKRLQNNPKVKFALCTIKGKVTGEWISGTVKILDGNQTKSTIEMRDKKYGFMAKIAKFLSKSKGELCAFSIKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSS2</accession>
    <name>A0A2S2KSS2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thrombospondin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_15210</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877415.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSS2"/>
    <dbReference type="GeneID" id="76208467"/>
    <dbReference type="OrthoDB" id="2442at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1080.10:FF:000005">
        <property type="entry name" value="Thrombospondin type 3 repeat"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1080.10">
        <property type="entry name" value="TSP type-3 repeat"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003367">
        <property type="entry name" value="Thrombospondin_3-like_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028974">
        <property type="entry name" value="TSP_type-3_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10199:SF119">
        <property type="entry name" value="RE20510P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10199">
        <property type="entry name" value="THROMBOSPONDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02412">
        <property type="entry name" value="TSP_3"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103647">
        <property type="entry name" value="TSP type-3 repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="444"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="663"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="768"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="456"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="792" mass="87027" checksum="77913C5CE5966F2E" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSS7</accession>
    <name>A0A2S2KSS7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_14940</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877290.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSS7"/>
    <dbReference type="GeneID" id="76209001"/>
    <dbReference type="OrthoDB" id="11558at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050595">
        <property type="entry name" value="Bact_response_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591:SF3">
        <property type="entry name" value="RESPONSE REGULATORY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591">
        <property type="entry name" value="STRESS RESPONSE REGULATOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="4"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="118" mass="13441" checksum="5ABA0299DB232DA3" modified="2018-07-18" version="1">MGLNVLVVDDSPFMRQNIKDVFRYMKINSVIEAKNGMDAIRAFKKYRPSLVTIDYEMPGMNGIETAMKIREIDKNVKMIIVTSIKSNMIAIKSGKIPQLGYITKPIDPFMIKNELAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KST5</accession>
    <name>A0A2S2KST5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carbohydrate kinase PfkB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_12990</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KST5"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050306">
        <property type="entry name" value="PfkB_Carbo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43085:SF57">
        <property type="entry name" value="CARBOHYDRATE KINASE PFKB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43085">
        <property type="entry name" value="HEXOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="4">
        <location>
            <begin position="55"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="306" mass="34432" checksum="834DAE9AE40FF520" modified="2018-07-18" version="1">MTKKMQKTKSLVSKIKKMKLAIFAHCAIDTISIDDSKYEQIGGSSCYCGITARQLKFDVDLFTKFGPDFPKQYLAENKINFINSESEKNTTKFLISINGSNRTLKLENECDPVDYSKIDADGHIVSPIFHEVSQDVFKKIKDDSNFLFVDPQGFLRQKDAQNNIFLQKTELDLSNVNAIKINPEEGECLVNGSHDEMMLSLQKKGVQYVLLTNKTDVSLLVKDKIYSITLPNKNIHDTTGIGDIFCSAFTCTMLKEKDFLWALSFAGGAAQAALDSKNIGLQKIPQKGTIQNNASYFYNLIKFRDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSV9</accession>
    <name>A0A2S2KSV9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_15050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877299.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSV9"/>
    <dbReference type="GeneID" id="76208991"/>
    <dbReference type="OrthoDB" id="4936at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="137" mass="14402" checksum="43275465ABEA6F9F" modified="2018-07-18" version="1">MDLKIIFFVGVLIITVSTIGLGSNLNSSITFNSIGSDDNISSRASEVDITNVVHSTSGNNIDSSTITIRNTDSVSHSYRICVITKAGSSISDTVGTAPDCATTLISSNNIDSVVVPFTNPLNKTTVDFADISIQEIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSY5</accession>
    <name>A0A2S2KSY5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 16">ATP phosphoribosyltransferase</fullName>
            <ecNumber evidence="4 16">2.4.2.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">hisG</name>
        <name evidence="19" type="ORF">NZNM25_15530</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + ATP</text>
            <dbReference type="Rhea" id="RHEA:18473"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="2.4.2.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.17" evidence="4 16"/>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSY5"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003879">
        <property type="term" value="F:ATP phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13593">
        <property type="entry name" value="PBP2_HisGL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013820">
        <property type="entry name" value="ATP_PRibTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001348">
        <property type="entry name" value="ATP_PRibTrfase_HisG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013115">
        <property type="entry name" value="HisG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00070">
        <property type="entry name" value="hisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03455">
        <property type="entry name" value="HisG_C-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403:SF10">
        <property type="entry name" value="ATP PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403">
        <property type="entry name" value="ATP PHOSPHORIBOSYLTRANSFERASE ATP-PRTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01634">
        <property type="entry name" value="HisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08029">
        <property type="entry name" value="HisG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 19" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="14" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 19" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP phosphoribosyltransferase catalytic" evidence="17">
        <location>
            <begin position="55"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine biosynthesis HisG C-terminal" evidence="18">
        <location>
            <begin position="247"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="NCBIfam" id="TIGR00070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF08029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="GBH34762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="330" mass="37941" checksum="EB62A07DA8D8A32A" modified="2018-07-18" version="1">MKKLKMSEVKFAIPKGSLEEATFKLLERSWTKVNRKSRTYRVYLDDPSIIVKMLRPQEIPTMVAEGLYDVGITGKDWVGETNSDVEAILDLEYGKIRLVIAFPDKYNYKNLDEMIADYGKKKKTLRISSEYLTTASKFLKQSKSYKKYHGSKDPLIVTPWVRLGTNKSVQIHLSFGATEAKPPEDVDAIMDVTETGTTLKQNKLKIVDEILTSTAHLIVNKNSLKDPKKREKIFDIVTLMRGAVHGRKYLHIYLNVEEKNLKKLLAEMPSLKRPTVSPLSEEGWFGINTVIKKEEFHKLIPKLRKIAQGLVVHEPRQILELEEIKRDEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT33</accession>
    <name>A0A2S2KT33_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proteasome assembly chaperone 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_15930</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877431.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT33"/>
    <dbReference type="GeneID" id="76208393"/>
    <dbReference type="OrthoDB" id="11427at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="113" mass="12532" checksum="1BCCD971025A5F73" modified="2018-07-18" version="1">MNSPNGFFQKAVNLESRNFFLQIQKFENGYFISITEGVKKIGSIVASLATGPSPITTTVIPTRTDSLFLKLVAERISTRVRGIALVSVFVQKELETDTAKALMSEIMDMIENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT57</accession>
    <name>A0A2S2KT57_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">6,7-dimethyl-8-ribityllumazine synthase</fullName>
            <ecNumber evidence="3 7">2.5.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_16100</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:26152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="2.5.1.78"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DMRL synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.78" evidence="3 7"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877447.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT57"/>
    <dbReference type="GeneID" id="76208376"/>
    <dbReference type="OrthoDB" id="7610at2157"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00404"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000906">
        <property type="term" value="F:6,7-dimethyl-8-ribityllumazine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd09211">
        <property type="entry name" value="Lumazine_synthase_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.960:FF:000003">
        <property type="entry name" value="6,7-dimethyl-8-ribityllumazine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="entry name" value="Lumazine/riboflavin synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034964">
        <property type="entry name" value="LS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00114">
        <property type="entry name" value="lumazine-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058:SF0">
        <property type="entry name" value="6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058">
        <property type="entry name" value="6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE DMRL SYNTHASE LUMAZINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="Lumazine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="NCBIfam" id="TIGR00114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="138" mass="15146" checksum="3C0C6C51BCC2DEDB" modified="2018-07-18" version="1">MNIAVVVSEFNEEVTSRMLAVAQEKAITLKLKILYTCKVPGAYDMPIIVDSLLQKNDVDAVVTLGAIIKGQTKHDEVISHSAAQSLTALSIKYQKPVSLGISGPGMQERHAYARIRPVAERAVEAVVKISYELKRIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT63</accession>
    <name>A0A2S2KT63_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PEFG-CTERM sorting domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_16510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829105.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT63"/>
    <dbReference type="GeneID" id="76208334"/>
    <dbReference type="OrthoDB" id="11778at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027560">
        <property type="entry name" value="PEFG-CTERM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04296">
        <property type="entry name" value="PEFG-CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="417"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="451" mass="51557" checksum="C86D931A97802E85" modified="2018-07-18" version="1">MSIRKYGILVFLTYLVFPASNVYGHGFGIDTISSIDIQGKELSISVEMPMYFENTQEQITITATEKETKENAKNVTFLIGLFHENEMIFRNYFFADNGILQIKVTPTQEEKITIHGEQDSLLGAWHGTESNPIEVTGPLFDSGGLYNFEIEVRTIDEPTNIIENSGIYNADLSLIEIISFIQKDSENNDVEFRSKSYFDTVSNFKYDFVAKEVTFEMPFDWSEKQISHIPVVHVETHLPKDFVEFLSPSYSAYVNGVELFKSSISVDDYTEDDERIIHLVLLQDHLRYLKNEMKKLDEPITENMVFRISASENTAFPLEAYTKSEDFKVNLSWDPLKVEPGIDTNFIFTIRDGKTNDPLRNSDYTFVLIQNGREIHRVSGTAQIGGEFEKFMFTDDQTGPTIIKFENIRNTGQETEFALVVVPEFGTITLLILVISIMSIVFVSRRNPLAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT68</accession>
    <name>A0A2S2KT68_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">A-type ATP synthase subunit I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_16610</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877486.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT68"/>
    <dbReference type="GeneID" id="76208324"/>
    <dbReference type="OrthoDB" id="85892at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016471">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF63">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="362"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="399"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="461"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="494"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="547"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="610"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="640"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="230"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH34870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="699" mass="77723" checksum="3F869A38AC9142DD" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT71</accession>
    <name>A0A2S2KT71_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Rhomboid family intramembrane serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_16340</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877465.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT71"/>
    <dbReference type="GeneID" id="76208350"/>
    <dbReference type="OrthoDB" id="26567at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050925">
        <property type="entry name" value="Rhomboid_protease_S54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731:SF14">
        <property type="entry name" value="PRESENILIN-ASSOCIATED RHOMBOID-LIKE PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731">
        <property type="entry name" value="RHOMBOID PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="8">
        <location>
            <begin position="87"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="258" mass="28606" checksum="DD7E7D35865C2EB1" modified="2018-07-18" version="1">MLPLRDENPHPPGFKPTVTYTLIIINVIVFFIEVAFTGQFFDFTNQNAFNLFYNWGAVPSCVTGGTVVNVDFGAGPLRVSCPDAPYFSLLSSIFLHGGLMHLGGNMLFLWIFGDNIEHKFGKIKYLGIYLMWGILAGLIHIYGDASSPIPAVGASGAISGVLGAYLVIFPKARIQTFLMMGFFWRMMHIQARWFLPFWLVFQNLLPFFIGGFGVAGGGVAYLAHIGGFVVGLATGYLYKKTHSSEFTYGTRYGYRPDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTA3</accession>
    <name>A0A2S2KTA3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Probable cobalamin biosynthesis protein CobD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">cobD</name>
        <name evidence="12" type="ORF">NZNM25_15780</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cell membrane</location>
            <topology evidence="2 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the CobD/CbiB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877406.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTA3"/>
    <dbReference type="GeneID" id="76208409"/>
    <dbReference type="OrthoDB" id="46105at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048472">
        <property type="term" value="F:threonine-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00024">
        <property type="entry name" value="CobD_CbiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004485">
        <property type="entry name" value="Cobalamin_biosynth_CobD/CbiB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00380">
        <property type="entry name" value="cobal_cbiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002281">
        <property type="entry name" value="PRK01209.2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34308">
        <property type="entry name" value="COBALAMIN BIOSYNTHESIS PROTEIN CBIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34308:SF1">
        <property type="entry name" value="COBALAMIN BIOSYNTHESIS PROTEIN CBIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03186">
        <property type="entry name" value="CobD_Cbib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH34787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="322" mass="35643" checksum="B1C7249BA6BB6503" modified="2018-07-18" version="1">MILESIFIVGIAIFIDLTLGDPKNKYHPTVWMGKLIATLTPIAKNKNPTIEKTGGILIIIITSLIVISLLLILNVGISLISFDYISIIVYVIVGGLLLKTTIAIRGMENHTHKILEFLDNDLDGARANLSMIVKRNTKNLDKNHVISGMLESISENTVDGITGPLFYFSIFGLPGAFMYRVINTADSMIGYRTDLFKNVGWFAATCDTILNYVPSRLTGLTMIISAAILKNNWKKSYKIMIRDGKKTESPNAGYPMAALAGALETKFEKINHYKLGDGEITLTKEHVYSAITMMKLTSILFFGIVTIPIISILSLIGWWIHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTB7</accession>
    <name>A0A2S2KTB7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Riboflavin synthase</fullName>
            <ecNumber evidence="3 8">2.5.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ribE</name>
        <name evidence="11" type="ORF">NZNM25_16120</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the dismutation of two molecules of 6,7-dimethyl-8-ribityllumazine, resulting in the formation of riboflavin and 5-amino-6-(D-ribitylamino)uracil.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.9" evidence="3 8"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877449.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTB7"/>
    <dbReference type="GeneID" id="76208374"/>
    <dbReference type="OrthoDB" id="10084at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004746">
        <property type="term" value="F:riboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00402">
        <property type="entry name" value="Riboflavin_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000003">
        <property type="entry name" value="Riboflavin synthase, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000004">
        <property type="entry name" value="Riboflavin synthase, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001783">
        <property type="entry name" value="Lumazine-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026017">
        <property type="entry name" value="Lumazine-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006767">
        <property type="entry name" value="PRK09289.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009566">
        <property type="entry name" value="PRK13020.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00187">
        <property type="entry name" value="ribE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21098:SF0">
        <property type="entry name" value="RIBOFLAVIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21098">
        <property type="entry name" value="RIBOFLAVIN SYNTHASE ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00677">
        <property type="entry name" value="Lum_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000498">
        <property type="entry name" value="Riboflavin_syn_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51177">
        <property type="entry name" value="LUMAZINE_BIND"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="Lumazine-binding" evidence="9">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Lumazine-binding" evidence="10">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Lumazine-binding" evidence="10">
        <location>
            <begin position="100"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="Lumazine-binding" evidence="9">
        <location>
            <begin position="100"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="NCBIfam" id="TIGR00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="194" mass="21080" checksum="0C61BE8AD07DEAF6" modified="2018-07-18" version="1">MFTGIVEGVGTVEKISKNTKNRSAIEMTVNLGKHSKGLKIGQSVALNGVCLTATKLSKSSCIFEMIEETTKKTDLGNLKVGGIVNIERSLKAGERLEGHFVLGHVDGVGIIKKILKKPKEVQVWFEVPKKLSKYVVKKGSIAIDGISLTVVDIKNSLASVSLIPHTLDITNFHTKKIGDKVNIETDILGKYILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTC9</accession>
    <name>A0A2S2KTC9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_16800</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_370684796.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTC9"/>
    <dbReference type="GeneID" id="76208306"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="36"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="114" mass="13193" checksum="AD5F85129B600A69" modified="2018-07-18" version="1">MLAKLRSSIVILIAVVIVGSVVIILGFLTNQEYVEISEISNQYEKLENYKNELEKINQYNQQILEDLEEQIKNSDDSSLEQIRKEIDVVKRVINDNKVELEKVIERLSQTESTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTE1</accession>
    <name>A0A2S2KTE1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Copper resistance protein D domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_17040</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877522.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTE1"/>
    <dbReference type="GeneID" id="76208282"/>
    <dbReference type="OrthoDB" id="4981at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006825">
        <property type="term" value="P:copper ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032694">
        <property type="entry name" value="CopC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008457">
        <property type="entry name" value="Cu-R_CopD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34820">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YEBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34820:SF4">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YEBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05425">
        <property type="entry name" value="CopD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Copper resistance protein D" evidence="7">
        <location>
            <begin position="51"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="165" mass="18332" checksum="E39D629B6F399286" modified="2018-07-18" version="1">MTALEQAILTWIHLVSAAIWVGGSLFIGIVFSPLLKTMTSSLEERMQIMIRVGRRFNKIAVPSLIILMATGLYSSHMLLGKPDLLVATSYGTFLIIKIILVIALIITYAIHVRVIRKDIEEKIMSNQMPESQIQKLRKKIIILGEITVVLSVAILFFASLLDAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTE3</accession>
    <name>A0A2S2KTE3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_17310</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829110.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTE3"/>
    <dbReference type="GeneID" id="76208254"/>
    <dbReference type="OrthoDB" id="6171at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="50" mass="5622" checksum="C8E35EF1DC1EDBAE" modified="2018-07-18" version="1">MAKYDGLLGQPVLEVEEPDKEDGITFILKDNRFLFIKAVDGKIETVSIPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTG0</accession>
    <name>A0A2S2KTG0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 8">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="1 8">6.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_17230</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="1 8"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877537.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTG0"/>
    <dbReference type="GeneID" id="76208262"/>
    <dbReference type="OrthoDB" id="8389at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000317">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023617">
        <property type="entry name" value="Tyr-tRNA-ligase_arc/euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050489">
        <property type="entry name" value="Tyr-tRNA_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006330">
        <property type="entry name" value="PRK08560.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264:SF4">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264">
        <property type="entry name" value="TYROSINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006588">
        <property type="entry name" value="TyrRS_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 9 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="NCBIfam" id="TIGR00234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="348" mass="39682" checksum="48BF3323E27AC72F" modified="2018-07-18" version="1">MDITEKIDLIERPPTEEVVTHDELIELFKSNSSPKHYIGLEISGFLHLGSLISTGLKINDFVKAGVKCTVFLADWHTLINDKLGGDWETISKVSKYYQDAFKLVCPEAKIVLGSKLYEEKAEYWSELVKFTKHMSIARTMRTLTIMGRSEDDKKIDLAKLLYPAMQAVDIHSLDVDIAHAGMDQRKIHMLVREIFPKMKWKVPVAIHHKLLPGLSKPVDTSDSQILGKMSKSDPNSGIFIHNTDDEIMKKISKAWCEEANIENNPLLEIARTVIFHDFDEIKVERPEKFGGNVSYTNYEQLETDFAQKKLHPGDLKQTVGNYLVKVISPIREKLNLSEEVNDAIRKSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTJ6</accession>
    <name>A0A2S2KTJ6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ubiquinone/menaquinone biosynthesis methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_16920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877513.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTJ6"/>
    <dbReference type="GeneID" id="76208294"/>
    <dbReference type="OrthoDB" id="8915at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="Methyltransferase" evidence="1">
        <location>
            <begin position="49"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="281" mass="31932" checksum="BA5025A496A48643" modified="2018-07-18" version="1">MSLLKSLEYKKRNITVWNEVAPRYHKRWASVSKGPFQSTTKLIELVEINKGDSVLDIACGTGVVTKNIRKKVGNSGYVVGVDTSSTAIKIAEKWSGKKSNVDFLNIDAENFNFSKKFDIITCQYALFFFPNADKALKNMKNSLKESGKIGISVHGRGDKVPFFSNILDSVKEFIPEYVPPGSPNLDRYGTKSALSTEISKAGFSEISVKEFIFHYSPGKFTDYWDNYIKYIAKPLKEKLNELDYSKRKELKQVVKEKTLQYTKKNGKILFPWQVLILTAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTJ7</accession>
    <name>A0A2S2KTJ7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KamA family radical SAM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_17470</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877555.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTJ7"/>
    <dbReference type="GeneID" id="76208238"/>
    <dbReference type="OrthoDB" id="21308at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003739">
        <property type="entry name" value="Lys_aminomutase/Glu_NH3_mut"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30538:SF0">
        <property type="entry name" value="L-LYSINE 2,3-AMINOMUTASE AQ_1632-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30538">
        <property type="entry name" value="LYSINE 2,3-AMINOMUTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01070">
        <property type="entry name" value="PLP-dependent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="454" mass="52261" checksum="729934722C9D153C" modified="2018-07-18" version="1">MTYQETIWDDTPSLKSYTLSNFRTLPQIQTLGEEAQFEMEVVGNVLPFKANNYVVEQLIDWNNIPNDPMFVLTFPQKGMLKPEHYEKMATALKNNLDKKEIASIANEIRLQLNPHPAGQMELNVPTLKDGTKLYGMQHKYKETCLFFPSQSQTCHAYCSFCFRWPQFVGMDEMKFAMQEGEQLVQYVREHPEISDVLFTGGDPMIMKAKIFSKYVDVLLDAKLPNLKTIRIGTKALSYWPYKFLTDSDSQEMLDVFRKIVDSEIHLAIMGHFNHLNELSTDAVKNAIKQIRSTGAIIRTQSPLLAHINDDAEMWAKMWTKQVQLGCIPYYMFVVRDTGAQHYFGVPLVEAYQIFKKAYSSVSGLARTVRGPSMSATPGKVHVIGTADLHDQKVIVLRFLQGRNPDWVQIPFFAKYDEHAIWLDDLKPALTEKFFFDEEMKNFKKSNPIDDYPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTL1</accession>
    <name>A0A2S2KTL1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH marR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_17740</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877579.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTL1"/>
    <dbReference type="GeneID" id="76208211"/>
    <dbReference type="OrthoDB" id="11435at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13463">
        <property type="entry name" value="HTH_27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="HTH marR-type" evidence="2">
        <location>
            <begin position="131"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="200" mass="23205" checksum="0786B0F80B186EE3" modified="2018-07-18" version="1">MLVEIPEPEVILGVILAFIVGLGGLYIYYKIRPFIKNKNEVFDASQSERLEYYERQLIDMKIRLDALEIQGIEEKSEDPDIDLKQFLEKLAKSDVKEELVEEQIIPKIEHEKPISVPNISPIEHSNPTNYVLQLITNKAMTSRDIQITLKRSREHTSRLMKKLFEDGYVQRNTESKPYTYSITEKGLAKVEEVQTKHSIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTN3</accession>
    <name>A0A2S2KTN3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_18210</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877620.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTN3"/>
    <dbReference type="GeneID" id="76210300"/>
    <dbReference type="OrthoDB" id="24596at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000047">
        <property type="entry name" value="NADP-dependent L-serine/L-allo-threonine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44196">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE SDR FAMILY MEMBER 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44196:SF1">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000126">
        <property type="entry name" value="11-beta-HSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH35030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="266" mass="29222" checksum="D751E3FCD012BADA" modified="2018-07-18" version="1">MNFKNKVVLITGASSGIGRETAIEFAKLGSNIILVARKIDKLEQVANELKKFNVTTFVCQCDVSKKEQVKEMSKIVLEKFDSIDILINNAGFAIYGSVSSLSIDDIESQMETNYFGMVYCIKNFLPSMLKKKSGHIVNIASVAASFGLPGIASYCASKFAMLGFSEGLKHELKNTGIGITVVSPIMVRTNFFDHSSFEKMPKYSPTSLDAKTVAKAILKAANSSRLEIVVPSVVRGAIWMKSTFPFFINPILGKSFKKQLDCTKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTN7</accession>
    <name>A0A2S2KTN7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_18050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877607.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTN7"/>
    <dbReference type="GeneID" id="76210316"/>
    <dbReference type="OrthoDB" id="10747at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="143" mass="16105" checksum="4BBC3E1049E4D590" modified="2018-07-18" version="1">MNKKIIVIPIVALIVVFVVNFSLEQPEREGNTVFHVTLADPNLYSNGVYSDSFVLDEGQYSFRFVPNGSSPEILSISLNGESFNFIEDFKLESTSHQTGISEYFTWKYTGKEILEISEKQEISIIINPNGKIMGSVSVYILQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTQ4</accession>
    <name>A0A2S2KTQ4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_16360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTQ4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="73" mass="7941" checksum="72C8D6BD54A63117" modified="2018-07-18" version="1">MYFSGFSSKTDGVILIESEACLFSMIMGGKTSPPNPCPNTFGIIANENIMQITVIEAKIFKIVLLRISAIKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTQ7</accession>
    <name>A0A2S2KTQ7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hemerythrin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_18000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTQ7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.520">
        <property type="entry name" value="nmb1532 protein domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012312">
        <property type="entry name" value="Hemerythrin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39966">
        <property type="entry name" value="BLL2471 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39966:SF1">
        <property type="entry name" value="HEMERYTHRIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01814">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hemerythrin-like" evidence="1">
        <location>
            <begin position="21"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="198" mass="23698" checksum="2B9D1B2D1818E41F" modified="2018-07-18" version="1">MIDFRYTLNQHLLYLSYLSATNQLRADHDQVRRLEKIVLKCSEELYKGTKIPFSDLTKITMIISEFIDSIHHSREEDSYFPCVASYDSLKEEIRKFMIEHEFGRNIARQISHHLKRWKEGEDAQEPVSRYLRTYAIFLNDHLTKENKFFDDAEANVLSQEEEIEMYEQYRSVFAIVKKVDEMIAEIDYLENQPWAKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTR5</accession>
    <name>A0A2S2KTR5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_18100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877668.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTR5"/>
    <dbReference type="GeneID" id="76210311"/>
    <dbReference type="OrthoDB" id="28494at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="343" mass="38258" checksum="17D9C927480430C3" modified="2018-07-18" version="1">MNPEIVPIHRKEFLREDGMLFVRTEGIIETMVKAPLIIASFIVVALAMPIQTSFSSTRTLDLILYSDGSVHVSSNLDVDPLNPDFEIKLFGPSIDNFVAVSENGFLLSSEIVDDKVTIDTFGSSSITIDYDIHDLISKEGRVWTFSLDSPTNYSLLMPQNSIIVGMNALPSNMIIVNDQTQLELSTGLSEINYILGTTNPPVISPPVTDPEQFSVDIFTISIIGVSIAAAIVGGIILIKRKQTQSLSILENESPKSETNIDSLDTETIFNLRPEMREDDKEIIKFISENGGQVLESDLRKKFLQPRTTMWRAVKRLERQGVIEISKKDLQNLVKLKKVLEDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTT3</accession>
    <name>A0A2S2KTT3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zinc-ribbon domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_18710</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877696.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTT3"/>
    <dbReference type="GeneID" id="76210098"/>
    <dbReference type="OrthoDB" id="12019at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="Zn_Ribbon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zinc-ribbon" evidence="2">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="176" mass="18618" checksum="D8EDCBEC6D55BD9E" modified="2018-07-18" version="1">MSFGEVDTLNMLFDKLQSLFDESQGYYESFLDTNNMYKKGQLSDKEFFQKLGDYTVAYSALEFLAIKVIFEMKKSMGSGSGTTQSPGLMPGMGNPGMMAGGMGVPPRAGTAQNPVGGGPPGIVSAKEAFGDVGTLPSPDPSLMPRRTTPHSENGCSSCGADLRPNAKFCTKCGTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTV7</accession>
    <name>A0A2S2KTV7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidylprolyl isomerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_18750</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTV7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049886">
        <property type="entry name" value="CFI_box_CTERM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041770">
        <property type="entry name" value="CFI_box_CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="240"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="273" mass="31252" checksum="4057C3603C35F874" modified="2018-07-18" version="1">MLLFGSAFAQTPFRDTAGLTKNGIEWCEENYQLYKIMGKDFFEHHKHSIESRLCGNLYNDDLWAYSEHDRYQKLIEQSRMYYNLEIQESVEESKEGIVDTNPVNVKEIPQEIEQQKKEQQQKELNESNIESKSEEVIIESPQIEEVNSEGGGCLIATATYGTELAPQVQLLREIRDNQLMNTHSGASFMAGFNQFYYSFSPIVADMERENPVFKEAVKIGITPLLSSLVILSHAESESEVLGYGIDVILMNVGMYFVAPALLFYGFQKRKDQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTX0</accession>
    <name>A0A2S2KTX0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Nitroreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_17060</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877524.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTX0"/>
    <dbReference type="GeneID" id="76208279"/>
    <dbReference type="OrthoDB" id="287850at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02145">
        <property type="entry name" value="BluB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.109.10:FF:000013">
        <property type="entry name" value="5,6-dimethylbenzimidazole synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="entry name" value="NADH Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012825">
        <property type="entry name" value="BluB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050627">
        <property type="entry name" value="Nitroreductase/BluB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02476">
        <property type="entry name" value="BluB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23026:SF90">
        <property type="entry name" value="IODOTYROSINE DEIODINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23026">
        <property type="entry name" value="NADPH NITROREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="FMN-dependent nitroreductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="7">
        <location>
            <begin position="17"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="236" mass="27227" checksum="EAB892CF1761B0EB" modified="2018-07-18" version="1">MTDDFTEDEKRGFYKAIYSRRDVRSHFTSKPIEDEILSKILHAAHHAPSVGFSQPWNFILIKDDQTKKKIKESFQDEKKRSSQLIEEPKRSKYLSFKLEGISESPVNLCVTYDPTKFGPFVIGRSSIPEAGLYSVCCAIQNLWLSARTEGVGLGWVSILSNETLKEVLELPEHVIPVAYLCLGYVDDFAEKPDLETAGWLPRLELKDVVYFEKWNDKENNNWKNIQKMIKDNLDYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTX4</accession>
    <name>A0A2S2KTX4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_18220</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877621.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTX4"/>
    <dbReference type="GeneID" id="76210299"/>
    <dbReference type="OrthoDB" id="9099at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="133" mass="15510" checksum="576A6D641E5DF31D" modified="2018-07-18" version="1">MVKKKEGDIPDWVTDEIQNAKFQKPQEMKKTGYVLEMYNEDNKIDAQLYDPVEDGRHIVTMDLSKKIKIDDLEKGVVYEFTFDQHKAPLSKKVSEYLEKEKEIEMNAIYQFELKSLELLDVGSSEAADDDLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTX7</accession>
    <name>A0A2S2KTX7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histidine kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_18950</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTX7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00075">
        <property type="entry name" value="HATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065:SF10">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065">
        <property type="entry name" value="SENSOR HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="137"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH35104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="354" mass="39832" checksum="09F87D918BE04CBC" modified="2018-07-18" version="1">MLIFALHVRLRIAKLPENEKQAMFFSENIQFETIKQFVIPLTLAIVCLVTIISLINVVFIQENKEISTQNLMLGIVAMLGVFVIIVLTINKNLSQLVKMRTDELVKQKNNLENIVEEKTHKLLKSERLSAIGELSGRLAHDLRNPLSVMKMSVDLINQSPHDTKISDSKVIERLDLIKKSIDRISHQVDEVLGFVRNSPIKLSSISLSELVENSTTKINVPKDIEIKFNKNNLKIKCDPIKLEAVVINLIVNAIQEMPKGGSVEIKTYEEKDNVILEFIDSGKGIPDKYLDKVFEPLFTTKQKGTGLGLASCKNIVEQHLGKISVKNDPTTFTVIIPKGLVEETSKIKNKLQFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTY5</accession>
    <name>A0A2S2KTY5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_18940</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTY5"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="78" mass="8578" checksum="CE7673236E3CD8B5" modified="2018-07-18" version="1">MPIIAGTNLIDANIGYVRAIDTAKESSPEISLYDTKRDGIKKEKDKAIANNQTFLLIELIIGLIERNKNAVINGYDAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTY6</accession>
    <name>A0A2S2KTY6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF998 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_19210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877734.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTY6"/>
    <dbReference type="GeneID" id="76210151"/>
    <dbReference type="OrthoDB" id="8148at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="197" mass="21518" checksum="51821D38E77425D6" modified="2018-07-18" version="1">MKLIQRLSNSTLTKSSTLVFLVSILAVLGPVVVVSAGFWDAISHLQKEPEFFWSPSHMVVYTGVSMTACAAIMGSMLILRRSVHGSLKTGIKLVIAGSIVQIIAGFGDSISHDLFGIDGLISWSHQPLELGLVLASLGGVLILKNREHTKLKLLLPFSIITFLFFTTWLIFNLVLIFGHTIQCIQVYEIFSSGCSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU56</accession>
    <name>A0A2S2KU56_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_19750</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the polysaccharide synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877784.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU56"/>
    <dbReference type="GeneID" id="76210205"/>
    <dbReference type="OrthoDB" id="4907at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003869">
        <property type="entry name" value="Polysac_CapD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051203">
        <property type="entry name" value="Polysaccharide_Synthase-Rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43318">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE 4,6-DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43318:SF2">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE 4,6-DEHYDRATASE (INVERTING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02719">
        <property type="entry name" value="Polysacc_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polysaccharide biosynthesis protein CapD-like" evidence="2">
        <location>
            <begin position="18"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="296" mass="33231" checksum="A330A36C344C5630" modified="2018-07-18" version="1">MDKIQKRALTKELKNKSVLVTGGAGSIGSVLVETLLEFPVQSVRVLDIDEYALAKLGKKLKDSRLRILLGSILDQDRVDMAADGIDIVFHLAAIKNIEISEFNPIETIDTNVNGTANIIKMLIKKRPSKFINISTDKAADASTLYGNTKQLGERLASWAGVHIKGTKSATVRFGNVIESRGNVFEVWNEEKKNNKPLSITEPSMKRYFIHSDEVIDFILKCLLMAKAGEVFIPKMQSFNIKKLADDISKSQKVIGLRRGEKLEEILLNNEEKSRAVEKSNMWIIKPYEKIVKNKMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU68</accession>
    <name>A0A2S2KU68_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PepSY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_19220</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877735.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU68"/>
    <dbReference type="GeneID" id="76210152"/>
    <dbReference type="OrthoDB" id="9699at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="79" mass="8793" checksum="AED405B5157BCF92" modified="2018-07-18" version="1">MSPGIGLMKRRLEKEKEAIALAVSGVAKKYDVKPDDIQTLETKYHDDAGDWYVALGWGEKKAVIQMDSVLGTITEIKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU86</accession>
    <name>A0A2S2KU86_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Daunorubicin resistance protein DrrA family ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_20050</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877811.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU86"/>
    <dbReference type="GeneID" id="76210239"/>
    <dbReference type="OrthoDB" id="31298at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050763">
        <property type="entry name" value="ABC_transporter_ATP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711:SF18">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH35214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="312" mass="35229" checksum="756973BD0A347991" modified="2018-07-18" version="1">MSCIDVKHLSKSYGSVHAVKDLELLVRPGQVFGFLGPNGAGKSTTIKLLTTLIPPSNGNLSILGIDAVANPLQVRHKIGVVLQQPSYEPTLSVEKSLDKYGMMWNVPKTERKKRMEQLLIDFDLVEIRKKRNEDLSIGQRRRIQVAREFMHDMELLFLDEPTVGLDPSARRNLLDYLKNKVKTGLTIFYTTHILTEAEYLCDEIAIIDKGRILTVDSPDALKKKFGKEKTIKIHLSEKQTNISSLLTGISDFKIDYENGTNIIIHSEQSELVLLKVLKILNDNTIEIEDLSAVPTNLEEIFLKMMRDNASNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU92</accession>
    <name>A0A2S2KU92_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pyridoxamine 5'-phosphate oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_19180</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877731.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU92"/>
    <dbReference type="GeneID" id="76210147"/>
    <dbReference type="OrthoDB" id="139492at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070967">
        <property type="term" value="F:coenzyme F420 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052019">
        <property type="entry name" value="F420H2_bilvrd_red/Heme_oxyg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35176">
        <property type="entry name" value="HEME OXYGENASE HI_0854-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35176:SF6">
        <property type="entry name" value="HEME OXYGENASE HI_0854-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="PNPOx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyridoxamine 5'-phosphate oxidase N-terminal" evidence="2">
        <location>
            <begin position="5"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="132" mass="15243" checksum="E8EF993287E5B28E" modified="2018-07-18" version="1">MGKRDEFLQSQKILRLSTIDKKTPHIVPVWYRYSGKKFYIGTNTKTEKAKNVKRNKHVAFCVDVGIKSPDIYGVMGQGEASLILENTKVKLIAKKILLRYFDDIENKSAKELLDDTDCIIEIIPKKFSVWSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU99</accession>
    <name>A0A2S2KU99_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein GrpE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">grpE</name>
        <name evidence="7" type="ORF">NZNM25_20000</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the GrpE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU99"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00446">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.22.10">
        <property type="entry name" value="Head domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01151">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000740">
        <property type="entry name" value="GrpE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013805">
        <property type="entry name" value="GrpE_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009012">
        <property type="entry name" value="GrpE_head"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237">
        <property type="entry name" value="GRPE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237:SF23">
        <property type="entry name" value="GRPE PROTEIN HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01025">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00773">
        <property type="entry name" value="GRPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58014">
        <property type="entry name" value="Coiled-coil domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51064">
        <property type="entry name" value="Head domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01071">
        <property type="entry name" value="GRPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="38"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH35209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="189" mass="21615" checksum="0E12EF0EB51A6288" modified="2018-07-18" version="1">MFELTSETNSDEIPVNVKSDNEIDSDESEESQTLSTNVEELAKLLDTEKEKVSEYEEKLKHVLADFQNLNRKTQSDIEKGVNAKIDEFVLDFLKIYDDFVIAKEVFSNNKINSDGLDSILKHMDSLLKKYHVVPIDALGEIFNPNFHEAISIINDPDLDDNTITKEIRKGYISHERVIRPTLVEISKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUA7</accession>
    <name>A0A2S2KUA7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">GDP-mannose 4,6-dehydratase</fullName>
            <ecNumber evidence="3 5">4.2.1.47</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">GDP-D-mannose dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">gmd</name>
        <name evidence="7" type="ORF">NZNM25_19620</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the conversion of GDP-D-mannose to GDP-4-dehydro-6-deoxy-D-mannose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>GDP-alpha-D-mannose = GDP-4-dehydro-alpha-D-rhamnose + H2O</text>
            <dbReference type="Rhea" id="RHEA:23820"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:57964"/>
            <dbReference type="EC" id="4.2.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the NAD(P)-dependent epimerase/dehydratase family. GDP-mannose 4,6-dehydratase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.47" evidence="3 5"/>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877772.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUA7"/>
    <dbReference type="GeneID" id="76210192"/>
    <dbReference type="OrthoDB" id="4907at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008446">
        <property type="term" value="F:GDP-mannose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070401">
        <property type="term" value="F:NADP+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042351">
        <property type="term" value="P:'de novo' GDP-L-fucose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05260">
        <property type="entry name" value="GDP_MD_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000924">
        <property type="entry name" value="GDP-mannose 4,6 dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00955">
        <property type="entry name" value="GDP_Man_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006368">
        <property type="entry name" value="GDP_Man_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43715:SF1">
        <property type="entry name" value="GDP-MANNOSE 4,6 DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43715">
        <property type="entry name" value="GDP-MANNOSE 4,6-DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="6">
        <location>
            <begin position="5"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH35171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="334" mass="38167" checksum="22E8D00DE252E6D0" modified="2018-07-18" version="1">MTRALITGITGQDGAYLAHFLLKKGYTVFGTFRRQSSSNFWRLYYLKIANKIKLIPADVLDSTSIYHAFEESNPTEIYHLAAQSFVGASFDQPLYTSDVTGFGTVRVLDEILKFNKKIKFYQASSSEMFGSEKSSIKNESTPFMPSSPYAISKLYAYWQTKLYREAYDLYAVNGILFNHESPLRGLEFVTRKISNEVAKISLGISTKLELGNISAKRDWGYAPEYVEGIWKSLQVKNPDDYVMATNESHSVKEFAEKACEIANISKKHISTSKKNLRPFDVNNLQGDYSKAKRKLGWKPKTKFNDLVKIMVQEDIHRWEQRLKGEYLPWDAGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUB9</accession>
    <name>A0A2S2KUB9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">dolichyl-phosphooligosaccharide-protein glycotransferase</fullName>
            <ecNumber evidence="6">2.4.99.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Oligosaccharyl transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">NZNM25_20200</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>an archaeal dolichyl phosphooligosaccharide + [protein]-L-asparagine = an archaeal dolichyl phosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.</text>
            <dbReference type="EC" id="2.4.99.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endomembrane system</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the STT3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.21" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUB9"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004576">
        <property type="term" value="F:oligosaccharyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003674">
        <property type="entry name" value="Oligo_trans_STT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048307">
        <property type="entry name" value="STT3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872:SF1">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT STT3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02516">
        <property type="entry name" value="STT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="136"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="164"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="326"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="356"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="381"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="414"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="Oligosaccharyl transferase STT3 N-terminal" evidence="18">
        <location>
            <begin position="11"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="GBH35229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="677" mass="74876" checksum="CCCE362F7E2F89B4" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUP5</accession>
    <name>A0A2S2KUP5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Capsule polysaccharide biosynthesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_19590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUP5"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015774">
        <property type="term" value="P:polysaccharide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007833">
        <property type="entry name" value="Capsule_polysaccharide_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05159">
        <property type="entry name" value="Capsule_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="496" mass="59241" checksum="8308EB4077BEA854" modified="2018-07-18" version="1">MKMKLLFWINVFMLHFGLSYYLKQKLDAEFTAIIDTPNKPKQMFLNQKIVDFRKTWYFHDHIKKTTVNPDLEYLSSFEKKYHIDLWKLALNERHFYRFNNFYKFTTNEILKFLEQECKLFEQILDEVKPDYFLTYDPPFHHQKLLMDLCKAKGIKVLCIFISRYKDKSIIAESGSTFDLPRNLDMIELNESEKNGLDEIMNESSYNALTKKWTNERNVSTSNKLKALTDYVLFSDSKNTQSNFTYYGRNKLKVIQDTISFYIKREWRSRYLEKNLKKVVDLDIPFVYYPLNIDAELTILHYAPFFTNQIEIIRHIAKSLPVDHMLYVKEHIHAVFRGWRETSQYKELIEIPNVVVIHPSYSSNELVKNSKLVITIRGTATIDAAYQNKPSIIFGDMAHDMLPSVYKIENLRDLPELIKTALRTPINSVYINKYLKLMKERTFDFNWWDYENKRNNQFYSGNILSDVEYQENDVKEFFDNNKEIFETLANAHLSKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNM7</accession>
    <name>A0A2S2KNM7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">50S ribosomal protein L3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_00210</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875903.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNM7"/>
    <dbReference type="GeneID" id="76209347"/>
    <dbReference type="OrthoDB" id="6121at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1430.10:FF:000005">
        <property type="entry name" value="50S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.960.10">
        <property type="entry name" value="Ribosomal protein L3, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045077">
        <property type="entry name" value="L3_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044892">
        <property type="entry name" value="Ribosomal_L3_dom_3_arc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_uL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019928">
        <property type="entry name" value="Ribosomal_uL3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_uL3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03626">
        <property type="entry name" value="L3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003261">
        <property type="entry name" value="PRK04231.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="NCBIfam" id="TIGR03626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="330" mass="36826" checksum="8010342DB88910E3" modified="2018-07-18" version="1">MGARKNHQPRRGSLAYSPRIRAKSMEARIRSWPKLNSEEPKILAHCGFKAGCVQIVTIDDREKVPNAGKQLVSLGTVLVTPPVLILGIRGYSKDHDGMHAEFDVYAEDIPKYISKEITVKNKEGALDNAEKSLRKIKEIFAIVAVSPRAAGLEQKKPYIFEASVSGGDIQKQFTHVKELLGKEIKIDQIFETGASVDVAAITKGKGWQGVIQRWGVKKKQHKSRKTVREVGSLGPISPQSVMYTVPRAGQMGFHQRIEYDKRIMIMGNTNDEQIKINPDGGYKHFGLVKGDFIILKGSVPGTYRRLIKLRSQIRNVPVKITKPNILEVVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNN9</accession>
    <name>A0A2S2KNN9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH arsR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_00440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875926.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNN9"/>
    <dbReference type="GeneID" id="76209373"/>
    <dbReference type="OrthoDB" id="371687at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12840">
        <property type="entry name" value="HTH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="20"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="166" mass="18832" checksum="90E65B639BAC5FAC" modified="2018-07-18" version="1">MSTLLEKQIKVNRIVTTSIEHARAIEDPARAKIMEILYHQALSADQISTALKKTGYKKALTTVRHHLEILKESGLVEIVRIEESRGAITKYYSTSTKLLDFQTPVDFDSTYSKVIHNTSTKIEKILKGLTPKTASKGKKSEDYSQYLVMEIMNRAMTNVLEKSNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNQ0</accession>
    <name>A0A2S2KNQ0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_00130</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875897.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNQ0"/>
    <dbReference type="GeneID" id="76209339"/>
    <dbReference type="OrthoDB" id="9006at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041017">
        <property type="entry name" value="Thioredoxin_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050553">
        <property type="entry name" value="Thioredoxin_ResA/DsbE_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42852:SF13">
        <property type="entry name" value="PROTEIN DIPZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42852">
        <property type="entry name" value="THIOL:DISULFIDE INTERCHANGE PROTEIN DSBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17991">
        <property type="entry name" value="Thioredoxin_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="15"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="368" mass="42178" checksum="8BE970E100A176A9" modified="2018-07-18" version="1">MKSEIKTALILGIVIAMGLVIISITFSTLDEKSETANIQEAKTLDKIDKSRFKMAPDLVGISHYLNTTPEELKKQIENKVVLYDIWTYSCINCVRTLPYITAWDDKYEEQGLLVIGIHSPEFEFEKNPENVKMAIEKYGIEYPVVMDNDMETWKAFENRYWPRKYIADHEGYIRYDHIGEGGYQETEKIIQQLLEERDASLGIQMASSGPLVELEEFEHTMFRTPELYFGYNFAQNRNQLGSDEGFQPEKIVSYSEPDKIDLHKFYPIGNWKNHEDSMELASANGSIKLLYNAKEVNLVTANNAELEIFLDGEPLPSKYSGKDIIFENKITVSEPGLYNIISSEISSSHTMEIKVVGKGFQIFTFTFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNV6</accession>
    <name>A0A2S2KNV6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01000</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875975.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNV6"/>
    <dbReference type="GeneID" id="76209428"/>
    <dbReference type="OrthoDB" id="11792at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="134" mass="14775" checksum="D689E20694232DAB" modified="2018-07-18" version="1">MSVNPDLLESLILFIKNNTPKYSLNLNGVLYPMINVSIANSPVPVNEPTTRGGVYFSDTFAYKMKGVVEDLSIVPLLTKKMLGPNTEFGELQIVTEIESEGKPQNLEIFTNLTNSVQTPNSIELSMIIVKLNSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNV7</accession>
    <name>A0A2S2KNV7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phage protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00450</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875927.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNV7"/>
    <dbReference type="GeneID" id="76209374"/>
    <dbReference type="OrthoDB" id="1315at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="100" mass="11565" checksum="8F0FFC10AECE00C5" modified="2018-07-18" version="1">MGIEDKKEIETLMNIVINQIPSYTNMINSEHWDVNLDDCILGMVYHSFVAKSTDYLNNKRTDNKQETNAESTFEMMNLISEVFNDRLADIKQEIISSLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNW2</accession>
    <name>A0A2S2KNW2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01510</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876020.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNW2"/>
    <dbReference type="GeneID" id="76209481"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="257" mass="30409" checksum="44143899BAD42130" modified="2018-07-18" version="1">MSQYRSIQKIILKNLPEVQQMPSQKRKKVLKNFLRGIITEVVKKVNLPDPLEIKSKIIKEQYLDKRRPKGHKKGEWKLDYNLWRMLYEAKLELNMRIDQNQIQEVGNMINYADYSSYDNGYLDNGDSLSQLIQASNSTTTAKQTHILRMDESLEKKTVNNRTYQKFVGAIDTALRTQSTIKLGQANFYLYEKTDLENNRWLKLILDVEILHDSFENKMTKWEMLRNVIDTEIKTIDIDKNSDELDILKKSFYINMVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNW8</accession>
    <name>A0A2S2KNW8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">homoserine dehydrogenase</fullName>
            <ecNumber evidence="2">1.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_01640</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the homoserine dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.3" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNW8"/>
    <dbReference type="OrthoDB" id="4488at2157"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00063"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00465"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004412">
        <property type="term" value="F:homoserine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000005">
        <property type="entry name" value="Homoserine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000554">
        <property type="entry name" value="Homoserine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001342">
        <property type="entry name" value="HDH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022697">
        <property type="entry name" value="HDH_short"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004912">
        <property type="entry name" value="PRK06270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004976">
        <property type="entry name" value="PRK06349.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43331">
        <property type="entry name" value="HOMOSERINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43331:SF1">
        <property type="entry name" value="HOMOSERINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00742">
        <property type="entry name" value="Homoserine_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036497">
        <property type="entry name" value="HDH_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="6">
        <location>
            <begin position="3"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Homoserine dehydrogenase catalytic" evidence="7">
        <location>
            <begin position="156"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="7"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>L-homoserine</name>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="336" mass="36342" checksum="71620C4EC2E0F991" modified="2018-07-18" version="1">MRIILCGFGVVGQSLLKLFESRSEDLYAKYGLKPRVVGVFDSKGSAVDSSGLEFNKLMDVKKKFGTVKNYSDKKNSMSGIEMLKNVEADVLIETTASNYKDAEPGMTHIITAMKKRMHVISVNKGPLALAFPSLLELAAYNQVMFKFSGTVGGGTPILDYAKKSLRGERITSFAGILNGTTNYILTNMATGMSFEEALKDAKNKGYVEADESLDLDGLDAAAKLVILANWIMGMKVTLPDINCTGIRNVTTEDIKKAEKNNCSVKLIASCNKELVVGPKEISKDDPLCVNGTLNAIAFTSEHSGTQTIIGRGAGGMETASSILRDLLDIRQEIART</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNY8</accession>
    <name>A0A2S2KNY8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">50S ribosomal protein L4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_00220</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875904.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNY8"/>
    <dbReference type="GeneID" id="76209348"/>
    <dbReference type="OrthoDB" id="10737at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_uL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_uL4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045240">
        <property type="entry name" value="Ribosomal_uL4_euk/arch"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19431">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="Ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="41"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="270" mass="29054" checksum="976A628B66BEB7BC" modified="2018-07-18" version="1">MKTASYSIAGVKDGEIELPLVFSTPFRRELIHKAFTNLTSHKFQPQGRHPSAGQDVVADSNDPPTGQGVARVARSSGGGGGRQGQGAEVASTRGGRQAHPPIVGKVIYKKLNKKENKLALCSAIAATGSKELVKLRGHKIDGIESFPIIVSDEIESMSKTNEIFKVLDALKLTQDVERLESRRPRSGQSRLRGRSKKIGKSVLFVTKDASNIKKAIGALPGVEAKSVKDLSILDLAPGSDPIRLTVYSKSAIEEIAKIKSTHLEVMVKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP26</accession>
    <name>A0A2S2KP26_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_01800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876048.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP26"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="66" mass="7194" checksum="1BFE7BE175A37A18" modified="2018-07-18" version="1">MATKRKAATKRRSTKTSTKAAPKRKAAAKRKAAPKRKTAAKKAAPKRKAAAKRKAAPKRKTAAKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP86</accession>
    <name>A0A2S2KP86_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_00960</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875971.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP86"/>
    <dbReference type="GeneID" id="76209424"/>
    <dbReference type="OrthoDB" id="115601at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF8">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="39"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="107"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="164" mass="18428" checksum="F3792A98442E45C2" modified="2018-07-18" version="1">MSDKVDKMLAQAFEFADEENFDDALRLYDLVLREEPDNINALVDKGVTLQNMGRIKQAIRTYDKALAISPNFLDAILNKGTALHSDQRYLEAIGCYDVALKIDKKCAMALAYKGLSLGEIGELQDAIKHFKKALSIDKDYDLANISKELAQDLLRSIKKKSKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP94</accession>
    <name>A0A2S2KP94_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0215 protein NZNM25_02530</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_02530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0215 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876116.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP94"/>
    <dbReference type="GeneID" id="76209587"/>
    <dbReference type="OrthoDB" id="15207at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2170.10">
        <property type="entry name" value="archaeoglobus fulgidus dsm 4304 superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00582">
        <property type="entry name" value="UPF0215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002802">
        <property type="entry name" value="Endo_dU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39518">
        <property type="entry name" value="UPF0215 PROTEIN MJ1150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39518:SF2">
        <property type="entry name" value="UPF0215 PROTEIN MJ1150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01949">
        <property type="entry name" value="Endo_dU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="193" mass="21430" checksum="8DC96B3C74DDFB7C" modified="2018-07-18" version="1">MRSLHLEKKGLRGLAIAESFGPNSSKSILSGIVMRRDFIIDDFVFGSATIGGDDATDSILKMYGELARPDVSYVLISGLIVSMYNIIDIKQLFDSLTIPIIGISYNDSMGISSAIKQHFPNSFESKLSEYDKLGKREKITLHTCHDVFVRREGCSLNDVRHLLDDLTLDGSIPEPIRVSQLLAKTLFEKGLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP96</accession>
    <name>A0A2S2KP96_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_01060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP96"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052048">
        <property type="entry name" value="ST_Response_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228:SF1">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ARR22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="1">
        <location>
            <begin position="9"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="127" mass="14121" checksum="33A60DE4CB224225" modified="2018-07-18" version="1">MSDAEMKHTCIIIDDMKSHQLLLSQMAEICGLEVIGIGENGKEAIDLFDSSKPDIVFLDMRMPKYDGFYAIDGIQKISETAKIIAVTADTTTDTFGRLTSMHVPVLSKPYSMDTLKKIVTDQFPSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPA4</accession>
    <name>A0A2S2KPA4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sugar isomerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00890</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875965.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPA4"/>
    <dbReference type="GeneID" id="76209417"/>
    <dbReference type="OrthoDB" id="34358at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="301" mass="33763" checksum="70393DB0F605B566" modified="2018-07-18" version="1">MNSIAAYEKDIELQLDFLKSFQKQNPISENLQKNTLFSGSGDSLASAMLAESFSNGLVKAMDPLDLYSNRELVKSKHVYFVSISGNTITNIRVATIAKKTTAITSQPKSKLAKISDDTILLTAGNHGVFTAGSISFLESALTCVSLVRKITIPKNNVLFLKAKSDAKKAKISKRLFVLGNFHTFPLAMYCAAKFYEILGYDSHYCRIEQFSHMELFSSKKGDTVIIFEAKNSHNTQLAQNLKKIGINVLHPNMPSEKISQMVYCIFFSQFISLNEAKRKKKRECHFVTAENIRNVSNQMIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPB1</accession>
    <name>A0A2S2KPB1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TldE-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_02870</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876142.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPB1"/>
    <dbReference type="GeneID" id="76209624"/>
    <dbReference type="OrthoDB" id="84520at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="entry name" value="PmbA/TldD superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047657">
        <property type="entry name" value="PmbA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43421">
        <property type="entry name" value="METALLOPROTEASE PMBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43421:SF1">
        <property type="entry name" value="METALLOPROTEASE PMBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="Putative modulator of DNA gyrase, PmbA/TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloprotease TldD/E N-terminal" evidence="2">
        <location>
            <begin position="19"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E C-terminal" evidence="3">
        <location>
            <begin position="219"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="320"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="452" mass="49849" checksum="43DDA9FEF437AB79" modified="2018-07-18" version="1">MSALEEALNHSKNVGVDECEIVVLKKKITTVRITDSEIAETKQSFEESYGIRLIHNKRIASIQITNQQKIKDVINDSLKTLHSVKPREFWRGLPSKIKSQKLDGTYDENLANISGSQAMDIAESMINATNSYKINTITGSLNIVSDDFELENSNGLKLREKSTYISGIINAESESGTLPVSGIGHDSCRTLSNFSTEQIGDDAKNMCLESINPQKIDSDTYSIIFEPYSVGELLSFVIASNFNFKTFAEKKSCFSNDFEKEIAVKQFSLIDDPHISEGIGTKSVDDEGVKTQKQSLIANGIFKNTFSNLYDSYKEGKQSSGNALRLGSPMGRSSEPIPISAPHNLKINPGKSTQENMIKDTKHGLLIGRLWYTYAVNPIKGDFSCTARSGIKIIENGEIKGPGKSVRIIHNLVNMLKNISEIGNNQKNIIQWASLPSIAPSIKAEKISVKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPD3</accession>
    <name>A0A2S2KPD3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ArnR1-like winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_02930</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876148.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPD3"/>
    <dbReference type="GeneID" id="76209630"/>
    <dbReference type="OrthoDB" id="10135at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="13504" checksum="119D27ABF937B42F" modified="2018-07-18" version="1">MMRARLTYVPLEVADQFGDFIIQRDEQVLDAVKARTRDFSTLSLIKLLYQLRGNPMTFSDLYSKSKIRMKKSFLNYLHLCVDYNFIKKEAVGSNMIYTITDKGRTMLNLFMQKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPE7</accession>
    <name>A0A2S2KPE7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01560</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876025.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPE7"/>
    <dbReference type="GeneID" id="76209486"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="88" mass="10501" checksum="1A7F6B3F9F6F4189" modified="2018-07-18" version="1">MSKWIHAKFSLDGSNRLISADRKTWFININQKYFKNMVSELVIKFTDLVCKVCMKSHHTQSGLISCLWFHRHDSIDYYLDNIMDDKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPJ8</accession>
    <name>A0A2S2KPJ8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">3-phosphoshikimate 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="7">2.5.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">5-enolpyruvylshikimate-3-phosphate synthase</fullName>
            <shortName evidence="7">EPSP synthase</shortName>
            <shortName evidence="7">EPSPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">aroA</name>
        <name evidence="9" type="ORF">NZNM25_03500</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21256"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="EC" id="2.5.1.19"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:21257"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EPSP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.19" evidence="7"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876198.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPJ8"/>
    <dbReference type="GeneID" id="76209690"/>
    <dbReference type="OrthoDB" id="43788at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00089"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003866">
        <property type="term" value="F:3-phosphoshikimate 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01556">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="entry name" value="Enolpyruvate transferase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00210">
        <property type="entry name" value="EPSP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006264">
        <property type="entry name" value="EPSP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01356">
        <property type="entry name" value="aroA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21090">
        <property type="entry name" value="AROM/DEHYDROQUINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21090:SF5">
        <property type="entry name" value="PENTAFUNCTIONAL AROM POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000505">
        <property type="entry name" value="EPSPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Enolpyruvate transferase" evidence="8">
        <location>
            <begin position="7"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="378"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="422" mass="45802" checksum="BFD70B60626DAF08" modified="2018-07-18" version="1">MKCKVEKSRISGKIVCPPNKSYTHRAIFLASLAGNNSKVENILHSADTIATIEACKKFGADLEIQNSAIIVKKSIKFDKNVPEINTENSGTTIRIASGIASLFSEEITLTGDDSLQKRPMQPLLDALSSMGAQCSSTDGKPPIKIKGKILGGDVKIPGNFSSQFISALLISAPLTEKGINLTIEGNLVSKPYLDATIATMRKFGITVQTLIPYKRYNIAPQIYKETTFIVPIDFSSLALLLSATVLIGDDITIKGNIGNLPQGDEAFIDILEQLGVSVSISEEEIKIKSPEKLTGGKFDLSNSPDLLPPLAILSLNSSAPIEIVNVKHARLKETDRIAIISRELVKIGIKIQEKEDGLILEKTDNLNGADLNSENDHRLFMSFCIAGMYVGNCIVTDPESVNVSYPNFIDEMNRLGAKIQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPM3</accession>
    <name>A0A2S2KPM3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">UPF0051 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_04030</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron-sulfur cluster assembly SufBD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876284.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPM3"/>
    <dbReference type="GeneID" id="76209749"/>
    <dbReference type="OrthoDB" id="300624at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055346">
        <property type="entry name" value="Fe-S_cluster_assembly_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011542">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045595">
        <property type="entry name" value="SufBD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01981">
        <property type="entry name" value="sufD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508">
        <property type="entry name" value="FES CLUSTER ASSEMBLY PROTEIN SUF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508:SF1">
        <property type="entry name" value="UPF0051 PROTEIN ABCI8, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19295">
        <property type="entry name" value="SufBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="Stabilizer of iron transporter SufD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD N-terminal" evidence="4">
        <location>
            <begin position="98"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD core" evidence="3">
        <location>
            <begin position="175"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00043967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="466" mass="52677" checksum="63E4B85DA3736BB7" modified="2018-07-18" version="1">MSQESLSKLNTSHIDEISSSRNEPEWLKDYRKSSLSIYDSLPIEMSPLYNKYTDAKKMDPEKVLLSTSTTETIPSFLNKRLTELENETCIIQIGTNVHKINISDDLKSKGLVISSISDAIQNNFELVKKALEASNSNDDKFTALNNAAFNSGVFIHIPSNLILEKPIHFLACLSEDGHSTIARNIIFADENSKATIVQEIYSPKTDKQPAYLELLNASVAANAQLDVTTLQLMDQHAVNFSTRRTDLAQDAKVNWYSGLFGSILSRYKIEYFLNGNGASANDSEVIFGNDEQSFDIQTNVNHESPSTEARVVEKSILRNKSKSLFKGMIRIKEHAAKSNSFLSGRSILLDKDAKSDAIPGLEIFTNDVKATHSASVAQIDEEQIFYLKTRCLTHEEAERTIVEGFLEPLSRKMSFQVRAWIAYLIESKWENRELTINTDAELAKFVEIEETRYNEDAEIEQHYKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPM6</accession>
    <name>A0A2S2KPM6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Signal recognition particle protein Srp19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_03800</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876225.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPM6"/>
    <dbReference type="GeneID" id="76209722"/>
    <dbReference type="OrthoDB" id="56356at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.30">
        <property type="entry name" value="Signal recognition particle, SRP19-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002778">
        <property type="entry name" value="Signal_recog_particle_SRP19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036521">
        <property type="entry name" value="SRP19-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01922">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69695">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0733">Signal recognition particle</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="11609" checksum="42780100DD7FD2E4" modified="2018-07-18" version="1">MKDYEHIVIWLDYFNKNLKKSKGRRVGLEKCVFDPSLKELMDATKDAGFEITESNDKVRFPKRPFVRSGYVILPKESSKTKILNKISEKLVSKRSKQTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPP0</accession>
    <name>A0A2S2KPP0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">P-II family nitrogen regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_02920</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876147.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPP0"/>
    <dbReference type="GeneID" id="76209629"/>
    <dbReference type="OrthoDB" id="10960at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00340">
        <property type="entry name" value="PIIGLNB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00938">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="22096" checksum="771929E63C446EFB" modified="2018-07-18" version="1">MKKIEAIIKRKTFTTIKTNLNVLGTFVIDKRNLDDSDIYDEAKGSRIGSTGIKSIPLAKIEIVVPDKEARKVVEMISKNSGLTSDHGGKIFVSEMEEVVDMETLDAKQDLEIFAEEKTKSAPIPKRSRFVPLQKFTLHKLQIVYEVNKEKLRDDYRIKSFSDFVNYCIVKSLPIMEKQLKNPTVIYENTFGDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPP4</accession>
    <name>A0A2S2KPP4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Iron-sulfur cluster scaffold-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_04000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876282.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPP4"/>
    <dbReference type="GeneID" id="76209746"/>
    <dbReference type="OrthoDB" id="319865at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06664">
        <property type="entry name" value="IscU_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002871">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10093">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY ENZYME NIFU HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01592">
        <property type="entry name" value="NifU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82649">
        <property type="entry name" value="SufE/NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NIF system FeS cluster assembly NifU N-terminal" evidence="1">
        <location>
            <begin position="8"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="147" mass="16353" checksum="23B646713B63F626" modified="2018-07-18" version="1">MSGNADIYHEMIVDYSRNPINYGEIENPDVTFHDSNPLCGDSIDIDMKIDENKISDIKFHGKGCAICMACSSVLTEITKGKTLDEARAIEKNDVLSELGLEHLQAVRIKCALLSLKVLKSALYTYIGKHLEDTSDVDKLKEEAANLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPR9</accession>
    <name>A0A2S2KPR9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_04300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876306.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPR9"/>
    <dbReference type="GeneID" id="76209778"/>
    <dbReference type="OrthoDB" id="115601at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="39"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="119" mass="13693" checksum="90ED6E5F98D8E6C8" modified="2018-07-18" version="1">MVGLFKHPKRRLKKLLKDGEYSDAIKLGKSLESEFSDDHDFMFIMGSVYLIVDDAKKALPYFDKAFELDNTDVENLTLKTNVHLALEQKDEAIDCCKRILKLQPENSEAQDLLEELESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPW0</accession>
    <name>A0A2S2KPW0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="12">2-oxoacid ferredoxin oxidoreductase subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_04840</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876353.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPW0"/>
    <dbReference type="GeneID" id="76209836"/>
    <dbReference type="OrthoDB" id="30755at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03375">
        <property type="entry name" value="TPP_OGFOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053399">
        <property type="entry name" value="2-oxoacid:Fd_oxidored_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051457">
        <property type="entry name" value="2-oxoacid:Fd_oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011896">
        <property type="entry name" value="OFOB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032686">
        <property type="entry name" value="PFO_beta_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041171">
        <property type="entry name" value="Oxoac_fdxbeta_Archa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02177">
        <property type="entry name" value="PorB_KorB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48084">
        <property type="entry name" value="2-OXOGLUTARATE OXIDOREDUCTASE SUBUNIT KORB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48084:SF2">
        <property type="entry name" value="PYRUVATE FERREDOXIN_FLAVODOXIN OXIDOREDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12367">
        <property type="entry name" value="PFO_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="10">
        <location>
            <begin position="49"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate ferredoxin oxidoreductase beta subunit C-terminal" evidence="11">
        <location>
            <begin position="200"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH33693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="320" mass="35081" checksum="C7F889E1017640AA" modified="2018-07-18" version="1">MALKLADYKTDVHNDWCPGCGDFGIVNAIQMALAEMGIQRDKATMFSGIGCSGKTSHYINTYGIHTLHGRVLTFAQGAKIANPEMTVVAVGGDGDGLGIGAGHFVAAGRRNVDMTYIIFDNGVYGLTKGQASPTLKLGEKTKSLPSPNTNFNVNPIGLAVASGFTFVARGYSYDVRHLKDLIIQAVKHKGLSFLDVLQPCPTYNDINTRDWYAGADLVDEAQKRHSRIYKLEEQGYDPVVHYDSVAEANEKLSQSLIKSLEWDTKIPIGVFYKNETISPYTQRLQDKIPNYLENPPAKQTISENGQPMTDISTILDSLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPW8</accession>
    <name>A0A2S2KPW8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_04800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPW8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="188" mass="22111" checksum="167AD0705C95025A" modified="2018-07-18" version="1">MKKLMEYLTTYPKTVSFLDGLKHKIHVDNKQGVEQLHVVVKKSFEELMKIFTAEGFTKVKFEHKQPGQIGNGLNLKLKKPWEMHIRMVDLKKGLIGIHAEVEVSRDYLQHLFSQRTPVVYEVEEILKKYYIDYSLWHDKIKKNVHVIVDNYKVKLATPSIPVFAWKPMLFVIGTIAAFYGWKYFNTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPX8</accession>
    <name>A0A2S2KPX8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF309 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPX8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3450.10">
        <property type="entry name" value="TTHA0068-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005500">
        <property type="entry name" value="DUF309"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023203">
        <property type="entry name" value="TTHA0068_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34796">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34796:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03745">
        <property type="entry name" value="DUF309"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140663">
        <property type="entry name" value="TTHA0068-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="174" mass="20010" checksum="00864338A346E43A" modified="2018-07-18" version="1">MPQNCRELAHKARDLASDMKNVSVRLARVATKFIEFDVAAEKEDLDALVDKLSPIGEIDNVRHVFEEHIEKEKGIEDGVFFFNNERFWECHEAFEGVWNQCYGREKELVQGIILVAVAFAHEQENEESIGIGMLSRALEKLGSSPSMYHSIDVDRIRKKAVEMQQAKKLTRFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPZ4</accession>
    <name>A0A2S2KPZ4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutamate-1-semialdehyde 2,1-aminomutase</fullName>
            <shortName evidence="8">GSA</shortName>
            <ecNumber evidence="8">5.4.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glutamate-1-semialdehyde aminotransferase</fullName>
            <shortName evidence="8">GSA-AT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hemL</name>
        <name evidence="10" type="ORF">NZNM25_04080</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>(S)-4-amino-5-oxopentanoate = 5-aminolevulinate</text>
            <dbReference type="Rhea" id="RHEA:14265"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="5.4.3.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.3.8" evidence="8"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866814.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPZ4"/>
    <dbReference type="GeneID" id="76209754"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00317"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042286">
        <property type="term" value="F:glutamate-1-semialdehyde 2,1-aminomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000021">
        <property type="entry name" value="Glutamate-1-semialdehyde 2,1-aminomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00375">
        <property type="entry name" value="HemL_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004639">
        <property type="entry name" value="4pyrrol_synth_GluAld_NH2Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00713">
        <property type="entry name" value="hemL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000818">
        <property type="entry name" value="PRK00062.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43713">
        <property type="entry name" value="GLUTAMATE-1-SEMIALDEHYDE 2,1-AMINOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43713:SF3">
        <property type="entry name" value="GLUTAMATE-1-SEMIALDEHYDE 2,1-AMINOMUTASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 8" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="5 8 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="8">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH33617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="424" mass="46847" checksum="2D82EBE35966155A" modified="2018-07-18" version="1">MTNSKLFSDSKKVIPSGVNSPVRYFEPYPFFTKKSNGAYLWDVENKKYIDFCNGYGALLLGHRRKEIINAVSNQLSKGTLYCTPTEAETELSKLIIGNFPSIDKVRLVNTGGEATMTAIRLARGFTKKKKIIKFEGCYHGAHDSVLVKAGSGSAHNGISVSEGGLDEVSKNTLVVQYNNIEDFQKTINQNKDIAGVIVEPILANMGLILPEKNFLYDLRKITKENNIPLIFDEVVTGFRVSPGGAQEHFGIKSDITTMAKALSNGFTIAAVGGRKEIIDLLSPGGKVYQASTFAGNPISVSAAISSIKTINKLKNKLYSKLERFNLLFTTALDDMATDMHIAHQINFTASMFQIFFTNKPVINYETSKKADSKKFQKMFRTLLKKGIFIAPSQFEVVFLSDAHTENDLNKTLDAYDAALKSVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPZ5</accession>
    <name>A0A2S2KPZ5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Acidic ribosomal protein P0 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl10</name>
        <name evidence="6" type="synonym">rplP0</name>
        <name evidence="8" type="ORF">NZNM25_05330</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 50S ribosomal subunit. Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which the L12 dimers bind in a sequential fashion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876394.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPZ5"/>
    <dbReference type="GeneID" id="76209884"/>
    <dbReference type="OrthoDB" id="30930at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000001">
        <property type="entry name" value="60S acidic ribosomal protein P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00280">
        <property type="entry name" value="Ribosomal_uL10_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050323">
        <property type="entry name" value="Ribosomal_protein_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022909">
        <property type="entry name" value="Ribosomal_uL10_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003099">
        <property type="entry name" value="PRK04019.2-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="7">
        <location>
            <begin position="111"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="288" mass="31803" checksum="DC6B6219A309E8A4" modified="2018-07-18" version="1">MHENRTSYPKRKTQMYQQLQELPKKYKVIAVIKLHKVRSTQILPLRKILKDDVQFVAIKDKVAQKALDSLDIPGIKEIAKDLEGQCMFLFTNMSPFKLNVLLAKNKVMMAARGGDIASIDIVVPAKNTGIAPGPMLTEFKEAGIPTKIDQGTIWIAKDSTPVLKGETINEKLASILGKLDIKPVEAGISLYTALEEGLKYAEEEMIIDVEKVRAQFSQAHQEAVSLSIEAAYITADNISQILTKAAQYARSLSIESGFMTDETKEQILQKANAQARAVAGQAKDYTPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ04</accession>
    <name>A0A2S2KQ04_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_05430</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876404.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ04"/>
    <dbReference type="GeneID" id="76209895"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="92" mass="10812" checksum="EE66CFC520E6F4F5" modified="2018-07-18" version="1">MTEFSCKQCNHPFEVFPPDKTFTKAESNECVVNGSGLMKCDMIREYRCENCYVVNHVYWHHNEGHDKKILKKAIELLGDNFSRSNAYKVEYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ31</accession>
    <name>A0A2S2KQ31_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_03560</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876204.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ31"/>
    <dbReference type="GeneID" id="76209697"/>
    <dbReference type="OrthoDB" id="43786at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008902">
        <property type="term" value="F:hydroxymethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01169">
        <property type="entry name" value="HMPP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004399">
        <property type="entry name" value="HMP/HMP-P_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019293">
        <property type="entry name" value="ThiN"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00097">
        <property type="entry name" value="HMP-P_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858:SF17">
        <property type="entry name" value="HYDROXYMETHYLPYRIMIDINE_PHOSPHOMETHYLPYRIMIDINE KINASE THI20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PHOSPHOMETHYLPYRIMIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10120">
        <property type="entry name" value="ThiN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine-phosphate synthase ThiN" evidence="2">
        <location>
            <begin position="261"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="430" mass="47427" checksum="38FF730E694E79A6" modified="2018-07-18" version="1">MNLLSIGGSDPSSGAGIQSDIKTFYTLNVHGLTIITAITSQNTSSFGNVEPVSQKILKNQIESIMTDFKIDGIKIGMVYNSQIIKILSKQLQKLKIPIVVDPVIKSTTGGALIEKSAMIDFQKYIIPLATVITPNRFEAEILSKIKINSKKSLRSAAKKIQKMGAKNVVITGIETGSKGISDFIFEKNKECFISGDKINLSNHGSGCNYSAAVIFALAKNKTIKESLRFAQQFTQNSIKNARKIGKGIAVTDVQDYISKDLSDAIEKFVHIKNIYKNIPECQINFVYSKQKPKSPEDILGISGRIVKSGKEAIVAGELTYGGSKHVATALLTMNKKYPKIRSAINLKYQDKTILKIKKSKLCISSYDRTEEPKNVKNKGSTIEWGIKKAVKDSTKMPDVIYHKGDFGKEPMIIVFGETPEKVLKKILKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ95</accession>
    <name>A0A2S2KQ95_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphate transport system permease protein PstA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">NZNM25_04160</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876294.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ95"/>
    <dbReference type="GeneID" id="76209764"/>
    <dbReference type="OrthoDB" id="11402at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005672">
        <property type="entry name" value="Phosphate_PstA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051408">
        <property type="entry name" value="Phosphate_transprt_permease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00974">
        <property type="entry name" value="3a0107s02c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42922">
        <property type="entry name" value="PHOSPHATE TRANSPORT SYSTEM PERMEASE PROTEIN PSTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42922:SF1">
        <property type="entry name" value="PHOSPHATE TRANSPORT SYSTEM PERMEASE PROTEIN PSTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="128"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="263"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="81"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH33625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="309" mass="33778" checksum="9A634F931D7FE39B" modified="2018-07-18" version="1">MSTNEKRQEYRTLFKNNVEKRLVVDKIVRIIVFACVIIAIIPLGSILVEVFKNGFAAISYEFLTEVPGSIGSGEGGIGPAIQGTMIIIGLSSLIGVPIGVMSGIYLSEYGDNRLARSIRFFNDVFMEFPSIVLGIFAFLVIVLLLGHFSVWAGAFALSLIMFPIVARTTEESLKMVPVTYREAGTALGLKKWVITFRIVISAAKSGMITGILLSVSRIGGETAPLIMTILGSSQFFSSMDVPMDALPLRIWRLSLLPYDSAQLQGWGSALVLIIIILGINLAVRYFFANRKGKPGIFRQLIKKGVHAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQB3</accession>
    <name>A0A2S2KQB3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_06440</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_263970054.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQB3"/>
    <dbReference type="GeneID" id="76210001"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="371" mass="42611" checksum="E7CA2BCCEBD0994A" modified="2018-07-18" version="1">MSGKDQSVVSKESLMSTKPGKQIIKQALFKSKGFKLFNKYKEETESEFPNFTERFAQGLLNEIKSDTNPNFTQQAFGDEVGSTEIILNASEIDPIKSKLETIEVIRDRVNRILNSNFVKMTFPVFNGLFDAAAEYSGRSDPKLKQDMVEGHILAIDLSEPMDRIVDKDEDLEFLDDYKLMNPYILKLARDKISKGGEQVLKEFEDGFKDARVGQYLDEKLKSKPTTITEEEMTLSYKKYRAVMGTAGRNMALAERPLGEIFYLGMARAAEGVGCGNEIEDSIKNGFVKIPSWPLYYSLLANDVKKGFEITMEKSNLYLQDARLTLELLPKDFTHKEFLEFLFLTVEHYNQFWYNKLQKINKWSDFESKLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQC3</accession>
    <name>A0A2S2KQC3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF541 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_06540</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQC3"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.170">
        <property type="entry name" value="Protein of unknown function (DUF541), domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2970">
        <property type="entry name" value="Protein of unknown function (DUF541), domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052022">
        <property type="entry name" value="26kDa_periplasmic_antigen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007497">
        <property type="entry name" value="SIMPL/DUF541"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34387">
        <property type="entry name" value="SLR1258 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34387:SF2">
        <property type="entry name" value="SLR1258 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04402">
        <property type="entry name" value="SIMPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="211" mass="22928" checksum="5250FBB070230DCF" modified="2018-07-18" version="1">MATASVDPDLLSVIFGIEIQKPTAKQALEENSKMMENVISALKSVGITESEISTSSLNIYPVYEYISDEFLGIQTRELVGYKVSNILSVETEKLNLAASIIDNGVDAGVNRVDSVYYSLSPQKQMDLKDDLLEQAITNAESKAKKALDPLDHTVIGVKSISLSEFGMPYPQPVFSGDFAMAESKIMSSTPVFSSEQDITTSIQVVFLIGSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQC4</accession>
    <name>A0A2S2KQC4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA repair helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04460</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876318.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQC4"/>
    <dbReference type="GeneID" id="76209797"/>
    <dbReference type="OrthoDB" id="11644at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09179">
        <property type="entry name" value="PLDc_N_DEXD_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="218" mass="25617" checksum="BEDD266370EDEF35" modified="2018-07-18" version="1">MGLRDIKLKEEYRSDRDDIVTEFFFPCLSHCIEYDRCVDFLSIHALTTISMAFENFSGGNAKLRMITGHRFRTADLNLFTKLFSEKFTRPFEGKSIQNSKIQKLQDIVNNGQIELKIVIPNSEQIANSFSERIGIFRDDDDQAVAFTGTSRESFSTQTRDFESVDVFTSWNDKSRVERKMQDFQDLWNNKTKHVEVYDFMFAEENNLLKYSSEWIMQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQD7</accession>
    <name>A0A2S2KQD7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sulfurtransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_04590</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876329.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQD7"/>
    <dbReference type="GeneID" id="76209811"/>
    <dbReference type="OrthoDB" id="9977at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01448">
        <property type="entry name" value="TST_Repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051126">
        <property type="entry name" value="Thiosulfate_sulfurtransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001307">
        <property type="entry name" value="Thiosulphate_STrfase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43855">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43855:SF1">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00683">
        <property type="entry name" value="RHODANESE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="13"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="149"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="252" mass="28702" checksum="06EFC4D870DD3E95" modified="2018-07-18" version="1">MLVSVDWLKEHLTDPDVVILDSRPKTMFLYGHLPNAQSLSVEQVIRFDQFGSNLVLEQEKIAELLGNLGIDETKTVVLVGDSMDPSVARIAWTFLYLGHEKTCLLDANVSDLQKYGFEFTKKLFAPKPAKFIPKINNAIRIESDFLKEHLDDFKILDARSPQEFMGGHLPNSKLIPFTEGIGYDGSLYQEKKFLDELFLQNNVSKEEAIVCYCMHGHRASNLFLQLKIAGFENVRLYDGSFVEWNGKKLPLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQE3</accession>
    <name>A0A2S2KQE3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Branched-chain-amino-acid aminotransferase</fullName>
            <shortName evidence="14">BCAT</shortName>
            <ecNumber evidence="14">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ilvE</name>
        <name evidence="15" type="ORF">NZNM25_06740</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Acts on leucine, isoleucine and valine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 14">
            <text>L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 14">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 14">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="14"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876512.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQE3"/>
    <dbReference type="GeneID" id="76210034"/>
    <dbReference type="OrthoDB" id="6469at2157"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00058"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00073"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00062"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052656">
        <property type="term" value="F:L-isoleucine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052654">
        <property type="term" value="F:L-leucine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052655">
        <property type="term" value="F:L-valine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01557">
        <property type="entry name" value="BCAT_beta_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000002">
        <property type="entry name" value="D-alanine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005785">
        <property type="entry name" value="B_amino_transI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033939">
        <property type="entry name" value="BCAT_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050571">
        <property type="entry name" value="Class-IV_PLP-Dep_Aminotrnsfr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01122">
        <property type="entry name" value="ilvE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005146">
        <property type="entry name" value="PRK06606.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42743">
        <property type="entry name" value="AMINO-ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42743:SF11">
        <property type="entry name" value="AMINODEOXYCHORISMATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 14 15" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="10 14" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9 14" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14 15" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU364094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GBH33883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="305" mass="34335" checksum="D6797483807B119C" modified="2018-07-18" version="1">MKLPLSKYVWFDGEYTLTEKAKVPITTHAIHYGTSIFEGIRGYWNGKNLYVFRLDEHVKRFRRSGQFYNISLNFSDKEITDAIVGICRKNTIKKSCYIRPFYFVGDYGINLHVTEKAPTNVAIFTFPFGDLFNKNGITAGVVSWRKFSDMSTPPQAKMGGNYLNSIIATQEAKRNGFDEAILLDHNGNVSEAPGENVFIVREGQLVTPALSSSALEGITRDAIIKIAKDLDIDVIERDVTRSELIISEEIFLTGTAAEITPIISMDAKKIGNGKPGDITKKMMQEYTDIVMNKNGDYSHWLTEVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQF4</accession>
    <name>A0A2S2KQF4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TIGR00268 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_05780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876432.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQF4"/>
    <dbReference type="GeneID" id="76209930"/>
    <dbReference type="OrthoDB" id="61764at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006163">
        <property type="term" value="P:purine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01990">
        <property type="entry name" value="LarE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005232">
        <property type="entry name" value="LarE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052188">
        <property type="entry name" value="Ni-pincer_cofactor_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00268">
        <property type="entry name" value="ATP-dependent sacrificial sulfur transferase LarE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43169">
        <property type="entry name" value="EXSB FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43169:SF2">
        <property type="entry name" value="NAD_GMP SYNTHASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006661">
        <property type="entry name" value="PP-lp_UCP006661"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD/GMP synthase" evidence="1">
        <location>
            <begin position="13"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="265" mass="29601" checksum="75F4C0BB318F3789" modified="2018-07-18" version="1">MTDLDDLVNWFEGKNKVMVALSGGVDSALVAYAAFQKLGDSAIAVTADYKTLSEEELTTARDVCSEIGIRQLFLDYNELENEYFTKNDSDRCFHCRMELGDHLIELAKEHDVQIIVDGTNVDDLGDYRPGIEALKQYGIRSPLVETGFSKLKIRDAAKSVGLSVFDKPSNSCLASRIPWGQRVTAEKLTRIEFGETIVKQLTKLKHVRVRDLNGSAKIEVDKDMISIFDESILEQITEKLKLVGFSSVEIDQEGYKPGKINVIAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQH5</accession>
    <name>A0A2S2KQH5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Enolase</fullName>
            <ecNumber evidence="6">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">2-phosphoglycerate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">eno</name>
        <name evidence="12" type="ORF">NZNM25_05980</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reversible conversion of 2-phosphoglycerate (2-PG) into phosphoenolpyruvate (PEP). It is essential for the degradation of carbohydrates via glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(2R)-2-phosphoglycerate = phosphoenolpyruvate + H2O</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds a second Mg(2+) ion via substrate during catalysis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Mg(2+) is required for catalysis and for stabilizing the dimer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell surface</location>
        </subcellularLocation>
        <text evidence="6">Fractions of enolase are present in both the cytoplasm and on the cell surface.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the enolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876448.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQH5"/>
    <dbReference type="GeneID" id="76209951"/>
    <dbReference type="OrthoDB" id="8680at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF1">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 6 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Enolase N-terminal" evidence="11">
        <location>
            <begin position="4"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="10">
        <location>
            <begin position="130"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6 7">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6 7">
        <location>
            <position position="331"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="358"/>
            <end position="361"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="382"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="382"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH33807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="412" mass="44447" checksum="92DD348485B1E851" modified="2018-07-18" version="1">MAKISSIEGRILYNSRGTKTIEVDVESEGKFVGRVCAPSGASVGKYEAVSFPSGKPEESLKILKENSKKFIGLESSDLKGIHNALKSLDNSTNYSNIGGALAFAVTIASMESASKALGQELFQILSQETSFKFPFPIGNILGGGAHAGPGTPDIQEILICATGSKTIEDAIETNLLVHKELRNVLEKEDPHFTNGRGDEGGWAPKLENMKALEVSAKACENLGFTLGKEVSLGVDFASSTQWNEEKGKYVYDRAGFENSPEEQIDFAAGIIEKFKLIYAEDAVHEEAFEGMAELTEKFPDILVTGDDLTVTNKDILTKAIDLKSCNAAILKVNQAGSLYDALEFANIANQNNIKLITSHRSGESTDSQISHIGLATKSKMLKVGVVGGERVAKLNELLRLSEHDLIRGMAEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQP6</accession>
    <name>A0A2S2KQP6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">phosphoribosylglycinamide formyltransferase 1</fullName>
            <ecNumber evidence="2">2.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">purN</name>
        <name evidence="6" type="ORF">NZNM25_07830</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.2" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876638.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQP6"/>
    <dbReference type="GeneID" id="76208892"/>
    <dbReference type="OrthoDB" id="27277at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004644">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08645">
        <property type="entry name" value="FMT_core_GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.170">
        <property type="entry name" value="Formyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01930">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004607">
        <property type="entry name" value="GART"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00639">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43369">
        <property type="entry name" value="PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43369:SF2">
        <property type="entry name" value="PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Formyl transferase N-terminal" evidence="5">
        <location>
            <begin position="6"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="205" mass="22719" checksum="0A184574464F492E" modified="2018-07-18" version="1">MLNLGILISGRGSNMENILKSIKSKKIPINPAIVISNKVDAPGLKIAEKLGIKTEIIESRGFEGSREAYDKKIIGSLTKYGVTPKNGLVCLAGFMRIISPQFVKKYKNRIINIHPALLPSFPGLNAQKQALEYGVKYSGCTVHFVDSEMDTGPVILQSVVKIKENDTEESLSKRILKEEHKIYPEVVNLFARKKIKVSGRRTIIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQT6</accession>
    <name>A0A2S2KQT6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DEAD/DEAH box helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_08140</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876662.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQT6"/>
    <dbReference type="GeneID" id="76208859"/>
    <dbReference type="OrthoDB" id="4631at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF13">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="9">
        <location>
            <begin position="2"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="33"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="213"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="372"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="5">
        <location>
            <begin position="2"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="384"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="406"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="438" mass="49012" checksum="FA55AD3E14AC970C" modified="2018-07-18" version="1">MTKFTELGLKDEILKGIKELGFEEAFPIQEAVIPVLLSGRDVVGQAHTGSGKTAAFALSMLQEIQPKNGIQGLIMAPTRELAIQISDEIKKFGKFTGIKVATVYGGQGMGIQLDALHRGVEIVVATPGRLIDHLKRGSIELRDVTHIVLDEADTMLDMGFVDDISFILDLAPEDRVMSLFSATMPTEILRLSEEYLKNPKQFLLDADDLSGEGIDQSFLVIKDRDKFKYLIDFIKQTKGQSIVFCSTKYRTRDVAKFLHQEKYNAVAIEGDMSQHRREQSMGKFRSGKADILVATDVASRGIDVPRVELVVNYDVPNQEMAYFHRIGRTARAGALGKAITFVSYSSVGDWNLIKRQIKVPIRDLNQEMGIQISIPDPLKRQTPSRRYGGQSRSNYSRGGRSGGYGRSDRSRNPRDDRGGKKRYGDRDNRNSYGGRSRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQT7</accession>
    <name>A0A2S2KQT7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Elongation factor 1-beta</fullName>
            <shortName evidence="6">EF-1-beta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">aEF-1beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ef1b</name>
        <name evidence="8" type="ORF">NZNM25_08410</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Promotes the exchange of GDP for GTP in EF-1-alpha/GDP, thus allowing the regeneration of EF-1-alpha/GTP that could then be used to form the ternary complex EF-1-alpha/GTP/AAtRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the EF-1-beta/EF-1-delta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876688.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQT7"/>
    <dbReference type="GeneID" id="76208830"/>
    <dbReference type="OrthoDB" id="84643at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00292">
        <property type="entry name" value="EF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00043">
        <property type="entry name" value="EF1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036219">
        <property type="entry name" value="eEF-1beta-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014038">
        <property type="entry name" value="EF1B_bsu/dsu_GNE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004542">
        <property type="entry name" value="Transl_elong_EF1B_B_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00489">
        <property type="entry name" value="aEF-1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001670">
        <property type="entry name" value="PRK00435.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39647">
        <property type="entry name" value="ELONGATION FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39647:SF1">
        <property type="entry name" value="ELONGATION FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00736">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006521">
        <property type="entry name" value="Transl_elong_EF1B_B_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00888">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54984">
        <property type="entry name" value="eEF-1beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation elongation factor EF1B beta/delta subunit guanine nucleotide exchange" evidence="7">
        <location>
            <begin position="3"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="93" mass="10415" checksum="D2D8C182A502EE9A" modified="2018-07-18" version="1">MTQLLLITKILPDGTEIDLDELAESIKGALKEGISMKRHAKEPLAFGLEFLKAEFILEDEEGQMDSLEDAIRSVKGVSEFEVLNMSRMSVDMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQV4</accession>
    <name>A0A2S2KQV4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07850</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876640.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQV4"/>
    <dbReference type="OrthoDB" id="9694at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="79" mass="9254" checksum="8EC8BB5740A5B9AD" modified="2018-07-18" version="1">MLEKQFNPDLLYNSIVHFYIDKKGYSKDKANAIAQSVVQKEAQRRICKNEKCKHFSHDHIRNTETCLVENCDCSKFSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR22</accession>
    <name>A0A2S2KR22_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_09030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR22"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="53" mass="6325" checksum="2864076D4B3CEA3E" modified="2018-07-18" version="1">MGFIIQYDHQPKCKYNPSILEFDKNIDPYQNTSRSKTTELDMDCQMSERHILT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR81</accession>
    <name>A0A2S2KR81_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thr operon leader peptide</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_09150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876750.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR81"/>
    <dbReference type="GeneID" id="76208755"/>
    <dbReference type="OrthoDB" id="2471at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="19294" checksum="BFCF4821223AC815" modified="2018-07-18" version="1">MNKHSIITGIAIIVIVIPFAVSGLNILGAQQLEYRWNNPGEFKFFTMSNHGEMEFCNTMPFWTNFQKFEIGAFYDSKNIGSFVVKPAAINPLSSHVQEGIFSSEEIQASQHIFMTLDFEFDGGDIRLDPNKFIILVQIDTPIIGLIPYSTTTQMSGFDFDKMMNSENLSCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR91</accession>
    <name>A0A2S2KR91_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_09830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR91"/>
    <dbReference type="OrthoDB" id="1680at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="529" mass="59348" checksum="49BAD156A8652C40" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRD4</accession>
    <name>A0A2S2KRD4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper-containing nitrite reductase</fullName>
            <ecNumber evidence="3">1.7.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_09180</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>nitric oxide + Fe(III)-[cytochrome c] + H2O = Fe(II)-[cytochrome c] + nitrite + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16301"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.7.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>Cu(+)</name>
            <dbReference type="ChEBI" id="CHEBI:49552"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotrimer.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.7.2.1" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRD4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050421">
        <property type="term" value="F:nitrite reductase (NO-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd11020">
        <property type="entry name" value="CuRO_1_CuNIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001287">
        <property type="entry name" value="NO2-reductase_Cu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00695">
        <property type="entry name" value="CUNO2RDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 10" id="KW-0186">Copper</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="11">
        <location>
            <begin position="92"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="type 2 copper site" evidence="10">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601287-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH34127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="470" mass="50803" checksum="67985770381E3EDE" modified="2018-07-18" version="1">MNKRVSMLFTIAAVAVMGATLFGSTYTQTQISGQSLDISKMDVNVIEQIHQMGGLQLVMPQAFAETDCGALESSGRTVVEFNLTGESVELPIMGGKTYNAMTFSGQVPGPTLRVTQGDVVKMTLTIPDDEVTGHGNDMHASQMSASNFESVNPGETSQYCYIAESAGVFKYHCSGVKLIGMDQHVLSGMYGIAIVDPANGYKKLMVEKTSGSGELDRKFYDADALEFQLQYNQLYLTPEGNYDAGAMFMHHNTATVVNGMQFGYVPNMAHNLLVNGDVKKNIFVAQPWNGIEHKQYQSQLLFVENDQHVRLFIENHGNEPVFFHIVGEILDRVTQGNRVQSGATETWLLGGSQNMIVDLVFDEPGAYAAVNHDYAAIYTGAATVFVAGDPFGLNPVLVEKGVIPAPVASYAYALGNPSDAVPPMGANSIAHPAINIHGLYTDEVASEMQASGDYVALWEVIPVVAEILTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRE2</accession>
    <name>A0A2S2KRE2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRE2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="104" mass="11766" checksum="68BB68FC207E8113" modified="2018-07-18" version="1">MSLLKPVSISKRILENKTNILNIPLRFVNFKRTKVITVTEIARMVRNPVFIPKIKKREDAISPACAVGRLNRSIFSGDAKRGTLKTALTKNKINSVIDTVLTWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRG7</accession>
    <name>A0A2S2KRG7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_09950</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_264953834.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRG7"/>
    <dbReference type="GeneID" id="76208672"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="205"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="446" mass="49121" checksum="0F23FEA84DE95F4A" modified="2018-07-18" version="1">MAFIKLIQPFRNIKVILKNNFDVNVYFLVILTLSCILILNVGTSYADVIPPRHQQKIGMSSDDVVCDSGLFKVIRERTNSVACVEPENVTKLVGMGWAKKVDDKQLSDMINRKSIQVGTINILEKIPVRTNVGKLASSTSISGYDIVFEVCASTSIYAPDVLVRSDSESKRYEMIETITANSCVLSVSKIKAADANSITVTLLNKGDISQKVLSLQNELDSLKQQLLDAKQSFKNPQASDAQIQGTKIADLRKQVNDKREELHRLLFTLHSPQTDKQKLEKFTFSGNVIEGESASILSVIESVQTPGLFDAIFEVCAGDTTVRLPVVTITSDKQTIKIKLGDKVSANSCQMTSGKIEADDKSSINITPAGNASSSNKVTELEMLIGSLTGQMVKEKQVLKDLVHDSNRASNFTELFDAQVSKIVELRNQITNAKAEFSKILYLTYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRH0</accession>
    <name>A0A2S2KRH0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08390</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRH0"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69304">
        <property type="entry name" value="Tricorn protease N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="800" mass="91743" checksum="B0D8BFF76778E787" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRH4</accession>
    <name>A0A2S2KRH4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Biofilm-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_10710</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRH4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF136">
        <property type="entry name" value="CD109 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0882">Thioester bond</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="711" mass="78099" checksum="19DA574C8D388212" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRI4</accession>
    <name>A0A2S2KRI4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_09680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRI4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="122" mass="13912" checksum="83007612146637E1" modified="2018-07-18" version="1">MADNHNNILSSMDLFIEKIQQLRGLMLGVSFSAVILAPLAIGISLYMMTHPKFLHLIENEGEFAILLTVLLTAVLVTSGIWLVAGIQQFRSLSNWNQRYTNYMKKRDDLDKDISSKFHLDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRL3</accession>
    <name>A0A2S2KRL3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF3139 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146195986.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRL3"/>
    <dbReference type="GeneID" id="76208784"/>
    <dbReference type="OrthoDB" id="2059at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="130" mass="14924" checksum="49C11492972ECF34" modified="2018-07-18" version="1">MKTRNLIITGILGVVLSFFVITGFLVFVVAPLNNQQITDYQSNELVKQFHETYPQSGMTTMSGTLFEDHISFTYFKNARYVSLTFSDGMDGQKITYKCERIISSQEPVEIFYFEEPTIQTIKDNVCGEYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRP5</accession>
    <name>A0A2S2KRP5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">NZNM25_10020</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRP5"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06225">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18773">
        <property type="entry name" value="PDC1_HK_sensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050398">
        <property type="entry name" value="HssS/ArlS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45528">
        <property type="entry name" value="SENSOR HISTIDINE KINASE CPXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45528:SF1">
        <property type="entry name" value="SENSOR HISTIDINE KINASE CPXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158472">
        <property type="entry name" value="HAMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="HAMP" evidence="15">
        <location>
            <begin position="325"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="14">
        <location>
            <begin position="403"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GBH34211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="603" mass="67904" checksum="280F85343C57ED8B" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRR1</accession>
    <name>A0A2S2KRR1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HVO-A0261-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_10220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876842.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRR1"/>
    <dbReference type="GeneID" id="76208645"/>
    <dbReference type="OrthoDB" id="11410at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057527">
        <property type="entry name" value="HVO_A0261-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25213">
        <property type="entry name" value="HVO_A0261_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HVO-A0261-like N-terminal" evidence="1">
        <location>
            <begin position="13"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="266" mass="31128" checksum="C625BC9194067B04" modified="2018-07-18" version="1">MPDLFDETANYALDLASPQRMQILFKLLEKNYTPTALAKEIDATKQEVHRNFVRLEESGLIEKETNGTYLLTTFGKIVCTQVPSLIFISQNRKYFEEHNFGEIPHKFQMRCGQLANGQYIKGISKVLEQWKSIYKNSNEYIYEILSEVPLDLIEPLVKQVKKGVRFNYIFSESASVPKGRKALLKKLGFDKLIEKGLIERKMEKNVQTVVILNEKEACIMFPTLDGESDLTEMFYSDDLMFHEWCLDYFRYCWYGSDVFRESKLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRR4</accession>
    <name>A0A2S2KRR4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_306307576.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRR4"/>
    <dbReference type="GeneID" id="76208491"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="177" mass="20202" checksum="E1801FA995C9FBE7" modified="2018-07-18" version="1">MMKYPGMGDPYKRNKTIKFLVISAIIGGIAVLLTSFVVNPMIGGQPHNACIDNIDTDWKISFTFEMILDGQKAEVQPNIGITEGCQRAIYTLSNDGTVYAEWTENPNFEIGHFLYISKFKIRDMEESKTEIYVNDRLSEMGLKTPLQDKYHYKAVFTSKDYDSSKDKDYLPELKTSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRS1</accession>
    <name>A0A2S2KRS1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876999.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRS1"/>
    <dbReference type="GeneID" id="76208489"/>
    <dbReference type="OrthoDB" id="12007at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="110" mass="11250" checksum="D248227C7E63C871" modified="2018-07-18" version="1">MNTKILLAASLVAVFTMGFYAEDAIAFAQYMGNVGSEGETGSYTLEEALEIQRRRIEAAEANPASGSGTPYLDASGVVGASVIAGAVFGGIAGAFFIRGRSGKYAAMGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRX0</accession>
    <name>A0A2S2KRX0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_10820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRX0"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="430" mass="46867" checksum="6C3B2DC84BEB86FD" modified="2018-07-18" version="1">MNFLTISLMAILVTGVIAPSLQYSNADVLPPNHQVDIGISTEDVVCDSGLYKVIRAANSSIACVKAKNVLKLIANGWATTVDKEPIDEEVLNTIINRKSIDLAQINILETVALKTQTGTAASGKSVSNYDVIFEICAYTPIYAPDVNVASDSETKHYELANLIDADSCVISVTKIKATDPTSIKIILLNKGDISEKVVMLQNELDSLKEQLTTIRSSLKVTDPDSQKQGVKIAELRKQINDKREQLHRTLFAIHLPSTAKDKIGEMTFSGNVIEGNSASVLSVLNATQTPGLYDAIFEVCAGSTTIKLPVIKVTSDKQSQTIKMGDKISANSCQMTSVKIEANDKSTISVTPAGNADSSNKASDLEVRINSLQASMIQEKQTLKSLIHNPDRPENFVELVDTHVIKIIELRNQLILAKAEFSKIMYQTYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS10</accession>
    <name>A0A2S2KS10_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_12610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829079.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS10"/>
    <dbReference type="GeneID" id="76209241"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="54" mass="6493" checksum="E228BA4844A233F2" modified="2018-07-18" version="1">MKQCKLCGTPLGKEPTAEELEKHWKKHHNWHWESNKEKTPEEALLKKRDQDVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS15</accession>
    <name>A0A2S2KS15_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamyl-tRNA(Gln) amidotransferase subunit A</fullName>
            <shortName evidence="7">Glu-ADT subunit A</shortName>
            <ecNumber evidence="7">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">gatA</name>
        <name evidence="9" type="ORF">NZNM25_12300</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="7"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877077.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS15"/>
    <dbReference type="GeneID" id="76209274"/>
    <dbReference type="OrthoDB" id="7931at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00132">
        <property type="entry name" value="gatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF7">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Amidase" evidence="8">
        <location>
            <begin position="25"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="7">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="481" mass="52126" checksum="658D18EB19602541" modified="2018-07-18" version="1">MNLKISALEYVQEVKNGNISAEDYVAKTIEHIQNVDDKLHAFLSVNNDAIDQAREIDKKIKSGKKVGDCFGMPISIKDNLCIKGSKTTCASKMLKDFIAPYDATAITKLKEQDAIFVGKVNMDEFAMGLTTEFSAYGPSKNPWNTEYVPGGSSGGSAVSVSAFECVASLGSDTGGSVRNPASFCSTVGYKPTYGLISRFGLISYANSIEQIGPLTRTVKDTAFMLNLISGIDPNDNTTIDNHQEDYLSGIDSGIEGKKIGIIQEMMGEGIDPSVLRATKEAISKLEGLGAICENVSLDMVKYSVAAYYTITATEAGSNLARYDNLRYGYDFPVEGYEFNSYISKARKNFGPEVTRRMILGGFVPSSGHAGKYFLKALKVKTKLTKEINDAFEKFDLLIAPTVPILPFKFGEKIDNPISLFLVDINTITANLTGKPAISVPFSHSNGLPIGIQLIANSNHDKLLLQAAYALEQTVTLPVVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS29</accession>
    <name>A0A2S2KS29_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_12050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877054.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS29"/>
    <dbReference type="GeneID" id="76209300"/>
    <dbReference type="OrthoDB" id="4910at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="125" mass="14468" checksum="A2F5FE3DD0F606A0" modified="2018-07-18" version="1">MDNLVIKHLQDLYGLNPNIPHMAFQFPRLPPGLTNPMFKIYENPMKEQFLKNAGTLNQKLQGFQQMYQQYAAGLLTMSSDVMPPGHPLYTRHNSVETLQNENDKLLKENLELKKKLDKESNSKTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS34</accession>
    <name>A0A2S2KS34_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Small ribosomal subunit protein eS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rps3ae</name>
        <name evidence="4" type="ORF">NZNM25_11680</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877004.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS34"/>
    <dbReference type="GeneID" id="76208484"/>
    <dbReference type="OrthoDB" id="30639at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00359">
        <property type="entry name" value="Ribosomal_eS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001593">
        <property type="entry name" value="Ribosomal_eS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030838">
        <property type="entry name" value="Ribosomal_eS1_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003142">
        <property type="entry name" value="PRK04057.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01015">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01397">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="203" mass="23645" checksum="2B4B3A62437AC18C" modified="2018-07-18" version="1">MARRKGRVKDKWREKRWITVNAPDSFNNVPIAYVPITDDENASGRVLEVTLYDILKGDPSQHQYKIYFQIDKVDGDKATTIFKRFEYSKEFLRSLVRRGSSKINFIIDIKTKDGYIFRIKVIALTHRQLNTSRKHALRLIARDVINKTVPEMTIEQFVQATCYSKINSDIMAAFKKVIRVRHVGLEKVKLIRTADKETVLLEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS47</accession>
    <name>A0A2S2KS47_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_12830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829082.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS47"/>
    <dbReference type="GeneID" id="76209217"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="47" mass="5717" checksum="9CEA89B9A7E05F45" modified="2018-07-18" version="1">MDYSNPNAEEDGVESRFDKLYPEYDYSLKSSSKEKDQRKKKRMKTMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS82</accession>
    <name>A0A2S2KS82_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12970</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877124.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS82"/>
    <dbReference type="GeneID" id="76209202"/>
    <dbReference type="OrthoDB" id="44at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="66" mass="7796" checksum="7BD06530221B8CBD" modified="2018-07-18" version="1">MDGPSEINSVYWDEHTKSWQYKIVKVEEYHGFVECQHCRKPMSHNVKSDGEFKVIYVKCGCTRNGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS92</accession>
    <name>A0A2S2KS92_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13410</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866894.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS92"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="144" mass="16473" checksum="3F995B1E0DA66C50" modified="2018-07-18" version="1">MEKVPDGIEKKIFENIFHTKSNKSKDHWYTLSDSYVNSVILQAFGDLEKKKIIESVSSSPKIINDILKDCQLPRTSGYRKINSLIDDGLIYQTEQIVVDNKKINKYACAFSNLKINIEKDKLTVDVQLSDSDILKSSIFQTIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSB1</accession>
    <name>A0A2S2KSB1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877145.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSB1"/>
    <dbReference type="GeneID" id="76209178"/>
    <dbReference type="OrthoDB" id="3049at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="62" mass="6949" checksum="50C3903F21B09A71" modified="2018-07-18" version="1">MDAKLDSTYLAITELTSEINSIVRKSFEKGNEELPSSDVEHILKITSDVACKIRPQLKELTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSB2</accession>
    <name>A0A2S2KSB2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">CTP synthase</fullName>
            <ecNumber evidence="3">6.3.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">pyrG</name>
        <name evidence="15" type="ORF">NZNM25_12950</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>UTP + L-glutamine + ATP + H2O = CTP + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CTP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSB2"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097268">
        <property type="term" value="C:cytoophidium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000009">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000002">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003792">
        <property type="entry name" value="PRK05380.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="CTP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550:SF0">
        <property type="entry name" value="CTP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTP synthase N-terminal" evidence="14">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase" evidence="13">
        <location>
            <begin position="295"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF06418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GBH34504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="521" mass="58063" checksum="D825123E4DEE6E45" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSH0</accession>
    <name>A0A2S2KSH0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1059 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13550</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877172.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSH0"/>
    <dbReference type="GeneID" id="76209144"/>
    <dbReference type="OrthoDB" id="9023at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009409">
        <property type="entry name" value="DUF1059"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06348">
        <property type="entry name" value="DUF1059"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="58" mass="6429" checksum="D8F71626D8AF26BC" modified="2018-07-18" version="1">MIKSISCSDAGKDCGWAATAPTEKELMEKVTQHVIESHKEIKLNTESISKIKSLIKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSQ1</accession>
    <name>A0A2S2KSQ1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">TldD/PmbA family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_14640</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U62 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877263.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSQ1"/>
    <dbReference type="GeneID" id="76209031"/>
    <dbReference type="OrthoDB" id="98233at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="entry name" value="PmbA/TldD superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051463">
        <property type="entry name" value="Peptidase_U62_metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025502">
        <property type="entry name" value="TldD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30624:SF0">
        <property type="entry name" value="METALLOPROTEASE SLR0863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30624">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN TLDD AND PMBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004919">
        <property type="entry name" value="TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="Putative modulator of DNA gyrase, PmbA/TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloprotease TldD/E N-terminal" evidence="5">
        <location>
            <begin position="24"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E central" evidence="7">
        <location>
            <begin position="115"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E C-terminal" evidence="6">
        <location>
            <begin position="229"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="470" mass="52478" checksum="DD059F87B3E7D429" modified="2018-07-18" version="1">MNSDLCELADKGIKYAMELGVHYCDVRAEKQDKKSVLIENKDIENIKTSTDAGLGIRLIKNGAWGFCSVTDPKSFEEIKNKIDHAVKNSSRHRKKKIETYSSTVNNVKIDFPVLKKPSIEKLMEIGLDCSQIILDTPKIIRSVINPWYTENSKYFTNSEGSKIEQNFTDMIIDMNAVAHDSGITQSINTTEGGRGGLEQITDKEKIQQKAKEISQKASELIIAKFAKEEQTTVVMNPDFVSLLTHEILGHPSEADRVLGKEMAWAGGAWWKGKIGEKIGSENLNVFDDPTIKESLGWYFFDDEGIKTQKTTLVENGILKKHLQNRETAQIFNTEPTGNMRATNYRFMPLIRMACTCIGNGDRNPDEIIKEVKKGILISNMKIPSIDMKRYNWSISCQYAQKIENGEITGLLRDVIVMGTAPEFFASINACGNDFTVRPITNCGKGDPMQSMIMGNGGPTIRGIATVKSVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSS3</accession>
    <name>A0A2S2KSS3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Type-4 uracil-DNA glycosylase</fullName>
            <ecNumber evidence="3">3.2.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">NZNM25_14650</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.</text>
            <dbReference type="EC" id="3.2.2.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the uracil-DNA glycosylase (UDG) superfamily. Type 4 (UDGa) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.27" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877264.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSS3"/>
    <dbReference type="GeneID" id="76209030"/>
    <dbReference type="OrthoDB" id="8612at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10030">
        <property type="entry name" value="UDG-F4_TTUDGA_SPO1dp_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051536">
        <property type="entry name" value="UDG_Type-4/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005273">
        <property type="entry name" value="Ura-DNA_glyco_family4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00758">
        <property type="entry name" value="UDG_fam4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33693:SF1">
        <property type="entry name" value="TYPE-4 URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33693">
        <property type="entry name" value="TYPE-5 URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00987">
        <property type="entry name" value="UreE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="12">
        <location>
            <begin position="29"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH34674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="197" mass="22021" checksum="324CF0D44D885C29" modified="2018-07-18" version="1">MEQELESIKQNVINCTKCDLCKTRTNSVPGKGNSQAEVMFVGEAPGRNEDKSGEPFVGIAGQKLSIALDEAGVSRESVYITNIVKCRPPQNRVPNTDERNTCQEYLKQEISIIKPKIICVLGNTAFSSILGGSEITKFRGKIVRRDNQLYFLTIHPAATIYNQGLIDVLKNDIVKLFENIRELKNGKEIKIDIEYDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSS5</accession>
    <name>A0A2S2KSS5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH hxlR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_13920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877205.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSS5"/>
    <dbReference type="GeneID" id="76209107"/>
    <dbReference type="OrthoDB" id="11945at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="19549" checksum="FB7FE2A25772C01D" modified="2018-07-18" version="1">METEPKDLIVLGAIKHGIKKFDKIQKTTQIEAEELNKILERLENRGFIKVVEKKGWLGKKIEIDVTDKGSREVDERVHELQTKWNQMSQIYKTGDKEKMKECMEENKSFLPTMMFFGVMDMVMFSMMFSMMGMAMSDYVPAESMPEGADTGMDDGSMDGGADDGGFDIDIGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KST0</accession>
    <name>A0A2S2KST0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD kinase</fullName>
            <ecNumber evidence="5">2.7.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ATP-dependent NAD kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nadK</name>
        <name evidence="6" type="ORF">NZNM25_12960</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>NAD(+) + ATP = ADP + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18629"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NAD kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="5"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877333.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KST0"/>
    <dbReference type="GeneID" id="76209203"/>
    <dbReference type="OrthoDB" id="350804at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF0">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="3 5" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="120"/>
            <end position="121"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="161"/>
            <end position="166"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="332" mass="36523" checksum="A05660A25859DD2D" modified="2018-07-18" version="1">MQIGIYGSGTAENAAKIIKKILDENAIKSFPITKSKTKPADCVIVLGGDKGVRNYFHRTFDSTSPVLGISEGEASGFLAQIELREFSSFVGILKKQNYTVEEVPRLGVKIDGKSVYPVLNDVAVFSSKSAMLMEHSLRVNGEEVWHDNSDGIIVSTPIGSSAYSMSAGGPVLFQDSAVFEIISVNSLDVTRRPIIVSNESLIEIDDVSARIHCEAILDGLDRFKVNKTVECTQFFPPAKIIRLKKDSTAISALAKKVHLAEELLSMPPSSKLLLKTLEYEGALTQKDLVNKTLLPDRTVRLALSHLLKKGYVKKKVSIRDARQKIYQISKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSX4</accession>
    <name>A0A2S2KSX4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_13390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877159.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSX4"/>
    <dbReference type="GeneID" id="76209159"/>
    <dbReference type="OrthoDB" id="2830at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17535">
        <property type="entry name" value="REC_NarL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058245">
        <property type="entry name" value="NreC/VraR/RcsB-like_REC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052048">
        <property type="entry name" value="ST_Response_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228:SF1">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ARR22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="1">
        <location>
            <begin position="7"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="129" mass="14408" checksum="61C6E2917EE34C18" modified="2018-07-18" version="1">MGLITKKCVIIDDDVQITELFSELLALLGLKILAIGHNGLDAIDLFSKHQPDILFMDVEMPKLNGIEALKEIKKINPSAKVIIITGNTSSDCERILQDNGVTAIIYKPFDIQKIKQLIEHFDNPVSMYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSX5</accession>
    <name>A0A2S2KSX5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">tRNA-intron lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_14480</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877251.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSX5"/>
    <dbReference type="GeneID" id="76209047"/>
    <dbReference type="OrthoDB" id="46045at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22363">
        <property type="entry name" value="tRNA-intron_lyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.20:FF:000001">
        <property type="entry name" value="tRNA-splicing endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1350.10:FF:000006">
        <property type="entry name" value="tRNA-splicing endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.20">
        <property type="entry name" value="tRNA intron endonuclease, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006677">
        <property type="entry name" value="tRNA_intron_Endonuc_cat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006678">
        <property type="entry name" value="tRNA_intron_Endonuc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036740">
        <property type="entry name" value="tRNA_intron_Endonuc_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006676">
        <property type="entry name" value="tRNA_splic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016442">
        <property type="entry name" value="tRNA_splic_arch_short"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00324">
        <property type="entry name" value="endA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21227">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21227:SF0">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01974">
        <property type="entry name" value="tRNA_int_endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02778">
        <property type="entry name" value="tRNA_int_endo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005285">
        <property type="entry name" value="tRNA_splic_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55267">
        <property type="entry name" value="tRNA-intron endonuclease N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA intron endonuclease N-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA intron endonuclease catalytic" evidence="4">
        <location>
            <begin position="86"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="178" mass="20488" checksum="3F5BD01CEB6E8925" modified="2018-07-18" version="1">MKSELIENRIIVWNIKDSRKLFSQGYYGKPIGIPKPKIDEIDAPLILDLIEGLYLLENKKITIRKSKQKITVEQLTDICRNEVHEFDKKYLVYKNFRDKGYIINPGIKFGCDFAVYEKGPGIDHAPFLIQVYTRNESISATGIVLAGRLATTVRKQFILAIPKGKNKVDFLALDWWKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT15</accession>
    <name>A0A2S2KT15_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2024 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_15700</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877399.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT15"/>
    <dbReference type="GeneID" id="76208417"/>
    <dbReference type="OrthoDB" id="5685at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.510.10">
        <property type="entry name" value="NE1680-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018592">
        <property type="entry name" value="DUF2024"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023122">
        <property type="entry name" value="NE1680-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09630">
        <property type="entry name" value="DUF2024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160766">
        <property type="entry name" value="NE1680-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="87" mass="10024" checksum="EDF6898080D74106" modified="2018-07-18" version="1">MDFHVFDTYVKAKDGHTMHFDVVTDESDIEKAISFAEEWLKGIGEETAKVSTEECRFCHTQSVPEDMEIEIMTNGYFIQRIDGCPEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT78</accession>
    <name>A0A2S2KT78_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Uridylate kinase</fullName>
            <shortName evidence="11">UK</shortName>
            <ecNumber evidence="11">2.7.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Uridine monophosphate kinase</fullName>
            <shortName evidence="11">UMP kinase</shortName>
            <shortName evidence="11">UMPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">pyrH</name>
        <name evidence="13" type="ORF">NZNM25_16710</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the reversible phosphorylation of UMP to UDP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>UMP + ATP = UDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:24400"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.22"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="11">Inhibited by UTP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the UMP kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.22" evidence="11"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877496.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT78"/>
    <dbReference type="GeneID" id="76208314"/>
    <dbReference type="OrthoDB" id="372251at2157"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00275"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033862">
        <property type="term" value="F:UMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006225">
        <property type="term" value="P:UDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000030">
        <property type="entry name" value="Uridylate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01220_A">
        <property type="entry name" value="PyrH_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011817">
        <property type="entry name" value="Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011818">
        <property type="entry name" value="Uridylate_kinase_arch/spir"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02076">
        <property type="entry name" value="pyrH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42833">
        <property type="entry name" value="URIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42833:SF4">
        <property type="entry name" value="URIDYLATE KINASE PUMPKIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005650">
        <property type="entry name" value="Uridylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/glutamate/uridylate kinase" evidence="12">
        <location>
            <begin position="3"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="8"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="114"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH34880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="227" mass="24830" checksum="17C7453DD758A534" modified="2018-07-18" version="1">MKKRIVIKLSGKIFGMDNVKVLKDYAEFLVKISNICQPIVIAGGGNIARHYISHARSSGADESTLDELGIEISRLNAKLLIYALKNKAYSHPPTTLQEVRHAVDDGLIVVAGGLHPGQSTNGTAALIAEKVQAEQFLNATDVDGVYDKDPNKYKNAKKFRRIELKNLKNMLIHEDSVAGGYDLMDIVALKIIERSKIKTRILKATPKNIESAIKGVNVGTEIVLGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT84</accession>
    <name>A0A2S2KT84_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PEFG-CTERM sorting domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_16440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829104.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT84"/>
    <dbReference type="GeneID" id="76208341"/>
    <dbReference type="OrthoDB" id="4392at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052721">
        <property type="entry name" value="ET_Amicyanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027560">
        <property type="entry name" value="PEFG-CTERM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04296">
        <property type="entry name" value="PEFG-CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507:SF1">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="289" mass="30456" checksum="57618FA29E41F046" modified="2018-07-18" version="1">MKTKAICSFFVLFAIVAGIVATTPAAFADHSEVTIETAPGSGAPGCEDTAEGCYIPSTATVDVGGKVIMSNVDTAAHTFTAGTPEDGPSGEFDTGLLMAGNSFEWSPDTIGEVPYFCMVHPWMQGLIVVQEAEAMDEHDDEHDDEMMGMEGAATATGMLSDGTMVSIWTSTPTAGEAMEISIEFEDAEHVNHDMMVTQNGVEVLMDEGAHHHEGTGMHTTAPLTSSDPVDITVTFQGYGVEDPKTGPIGEEVVFSNVVPEFGTIAMMVLAVAIISIVAVTAKSRVIPRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT85</accession>
    <name>A0A2S2KT85_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tetratricopeptide repeat protein 21A/21B N-terminal ARM repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_15580</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877389.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT85"/>
    <dbReference type="GeneID" id="76208429"/>
    <dbReference type="OrthoDB" id="115601at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056833">
        <property type="entry name" value="ARM_TT21_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047558">
        <property type="entry name" value="TPR_END_plus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF8">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25062">
        <property type="entry name" value="ARM_TT21_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Tetratricopeptide repeat protein 21A/21B N-terminal ARM repeat" evidence="4">
        <location>
            <begin position="15"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="40"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="199" mass="22754" checksum="1CE07A6DD8005CF1" modified="2018-07-18" version="1">MTDPKIDSILDEGNRLFLQGKLKEAIIYYDEILNENPLHLSSLNNKGYALSKLKDYENAMKCYDAALQTFPDDLSVLINKVSSFRKLGNFSKALSLCDEILKNNPNYNIALYHKERILFSMGKFDESILCCNSILNDYPNNGDVLFDKSCNLAMLSKIDEALDMLERAISQGLQYKIKAKKSKSFEKLFDNSRFNSMVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT91</accession>
    <name>A0A2S2KT91_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_16810</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877505.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT91"/>
    <dbReference type="GeneID" id="76208305"/>
    <dbReference type="OrthoDB" id="10550at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35902:SF3">
        <property type="entry name" value="NPCBM-ASSOCIATED, NEW3 DOMAIN OF ALPHA-GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35902">
        <property type="entry name" value="S-LAYER DOMAIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="405"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="434" mass="47718" checksum="8DA04A718807F6FF" modified="2018-07-18" version="1">MILKIMFALFVIGLVPFIFENSYAQIKSGGFGDSPFERDFGDVKFLDAYFGTINEKIEVDSGDSNVPFTVVFANVGTQDITGIRGQLSLPLGFSASDGPGSIIRADSDSNSLAGENFYLTFFVNLDKNIRIQQYPGTVKVDYSRLRESGVRTAFADFDFKVTGDIVINVRAIEPFLTSLRTNDVVIEISNNGTAPISGVDITATNTQTELASTSSSITNVENVVILESSWDIGTIDPKSVRYLTATVYVPESLRGDTLRLPLSISYFNAHGDEKVISKIVDFYIKGLIDLTVFNTDVIDLSGKQMVIGEIINEGNEDGLFGFVTIEPRGNSNIKSSTQFIDEIEVDAPVPFNVPIEFDGEPKYGEHDITITIRYKDSTRDEVFLTHDTTIFVKEPSNDDDGDLDSTLIVIPIILAVGAGIFIIRRRKKAKIEAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT96</accession>
    <name>A0A2S2KT96_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">3-isopropylmalate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_14760</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877275.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT96"/>
    <dbReference type="GeneID" id="76209019"/>
    <dbReference type="OrthoDB" id="6813at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019298">
        <property type="term" value="P:coenzyme B biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000019">
        <property type="entry name" value="Homoisocitrate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF34">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="7">
        <location>
            <begin position="3"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="339" mass="36988" checksum="024106675B7EB3CE" modified="2018-07-18" version="1">MYKISLITGDGIGPELSDSAISVFDTINDKLGLKFDVTELSAGDKALQETGKALPDDTVKTIKQSDACMKAPVGESAADVIVVLRRILDLYANIRPAKSYPHMPALRDDIDMVIVRENTEDLYTGKEFSLGNAAVALRIISEDASKRIAKYAFETAVQRNSLKKVTCVHKSNVMRITDGLFAKACTEISKNYPDVAFEEMYVDACAMNLIRQPEKFDVIVTTNLFGDILSDESSQVVGGLGMAPAANIGDNFALFEPVHGAAFDIAGKNIANPSSFLLSIKMMLDWLGNKHKDSKCIEIGTKLESTIFDLVKSGVKTKDIGGDMSTIDFTRHITDNMQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTF1</accession>
    <name>A0A2S2KTF1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_17130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTF1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="75" mass="8686" checksum="AA46E55EF6483731" modified="2018-07-18" version="1">MICIGIGYIHEYRMKIKCGCHCIKCNGTDLESNRISEIEKDGYFDMHHTCNQCNTHFDHLDGETFSICEKCNYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTH4</accession>
    <name>A0A2S2KTH4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_17200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877660.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTH4"/>
    <dbReference type="GeneID" id="76208265"/>
    <dbReference type="OrthoDB" id="6584at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="191" mass="21845" checksum="147D773F6CCCF7A4" modified="2018-07-18" version="1">MKPRHLEKTDSGYKVYLYVFYICGFIMLSSLVFGVYVTMIEWMENGTYVMGEAIHNTTIPFENFARVSTWIFFSTIIGWYCVSRIGWRRTAGNKIVGIKMALLQLMLLGFSIITLYEVIYNFTVLNAQMTAGIIDGMVPDIDRLSVAYPDPDRPWNLVFATKVFLAAFIISAHAFYLSIKPRKSLDISEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTH8</accession>
    <name>A0A2S2KTH8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA polymerase sliding clamp</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Proliferating cell nuclear antigen homolog</fullName>
            <shortName evidence="3">PCNA</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pcn</name>
        <name evidence="8" type="ORF">NZNM25_17270</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Sliding clamp subunit. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer. The subunits circularize to form a toroid; DNA passes through its center. Replication factor C (RFC) is required to load the toroid on the DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 4">Belongs to the PCNA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877540.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTH8"/>
    <dbReference type="GeneID" id="76208258"/>
    <dbReference type="OrthoDB" id="14749at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00577">
        <property type="entry name" value="PCNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.70.10.10:FF:000040">
        <property type="entry name" value="DNA polymerase sliding clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00317">
        <property type="entry name" value="DNApol_clamp_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000730">
        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022649">
        <property type="entry name" value="Pr_cel_nuc_antig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022648">
        <property type="entry name" value="Pr_cel_nuc_antig_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00590">
        <property type="entry name" value="pcna"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF0">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02747">
        <property type="entry name" value="PCNA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00705">
        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00339">
        <property type="entry name" value="PCNACYCLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 3 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA N-terminal" evidence="6">
        <location>
            <begin position="10"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA C-terminal" evidence="7">
        <location>
            <begin position="124"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="248" mass="27359" checksum="4E7CD89D859E233F" modified="2018-07-18" version="1">MTFGAKTSGSDDLKAIISAISTLVEEATFVATAEGITFRGMDPSHVALIDISWPNSAFEKYECDSDIKFGVRIDEFSKLIKRADKKDSIEISISEQNMLLVTVGKNKKYKMRLIESSATDTPLPKIPYDSKIILSSSKFDKILGDVQVVSDYLTIHTTDSKGDFSGKGDSGEVVIELEKEDEEIEEISSKEDSIGTYSLEYLNPVVKAVGTTVGFITCEFSSAKPLRIEFKVANIGRIHFYLAPRVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTI1</accession>
    <name>A0A2S2KTI1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DNA polymerase II large subunit</fullName>
            <shortName evidence="14">Pol II</shortName>
            <ecNumber evidence="14">2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Exodeoxyribonuclease large subunit</fullName>
            <ecNumber evidence="14">3.1.11.1</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">polC</name>
        <name evidence="18" type="ORF">NZNM25_17710</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 14">Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2 14">Heterodimer of a large subunit and a small subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 14">Belongs to the archaeal DNA polymerase II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="14"/>
    <dbReference type="EC" id="3.1.11.1" evidence="14"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877577.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTI1"/>
    <dbReference type="GeneID" id="76208214"/>
    <dbReference type="OrthoDB" id="7529at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00324">
        <property type="entry name" value="DNApol_II_L_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004475">
        <property type="entry name" value="PolC_DP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056172">
        <property type="entry name" value="PolC_DP2_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056171">
        <property type="entry name" value="PolC_DP2_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016033">
        <property type="entry name" value="PolC_DP2_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00354">
        <property type="entry name" value="polC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003103">
        <property type="entry name" value="PRK04023.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42210">
        <property type="entry name" value="DNA POLYMERASE II LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42210:SF1">
        <property type="entry name" value="DNA POLYMERASE II LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24846">
        <property type="entry name" value="PolC_DP2_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24844">
        <property type="entry name" value="PolC_DP2_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03833">
        <property type="entry name" value="PolC_DP2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016275">
        <property type="entry name" value="PolC_DP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 14" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="8 14" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 14" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6 14" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA polymerase II large subunit DP2 N-terminal" evidence="15">
        <location>
            <begin position="21"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase II large subunit DP2 central" evidence="16">
        <location>
            <begin position="304"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase II large subunit DP2 catalytic" evidence="17">
        <location>
            <begin position="703"/>
            <end position="999"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF24844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF24846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="GBH34980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="1125" mass="126407" checksum="AAAA5FA52D7AD2D4" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTI3</accession>
    <name>A0A2S2KTI3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase</fullName>
            <shortName evidence="5">HPPK</shortName>
            <ecNumber evidence="5">2.7.6.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">6-hydroxymethyl-7,8-dihydropterin diphosphokinase</fullName>
            <shortName evidence="5">6-HMPDK</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">7,8-dihydro-6-hydroxymethylpterin diphosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">7,8-dihydro-6-hydroxymethylpterin pyrophosphokinase</fullName>
            <shortName evidence="5">PPPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mptE</name>
        <name evidence="7" type="ORF">NZNM25_15660</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the transfer of diphosphate from ATP to 6-hydroxymethyl-7,8-dihydropterin (6-HMD), leading to 6-hydroxymethyl-7,8-dihydropterin diphosphate (6-HMDP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>6-hydroxymethyl-7,8-dihydropterin + ATP = (7,8-dihydropterin-6-yl)methyl diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal 6-HMPDK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.3" evidence="5"/>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTI3"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003848">
        <property type="term" value="F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004788">
        <property type="term" value="F:thiamine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02131">
        <property type="entry name" value="HMPDK_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027510">
        <property type="entry name" value="HMPDK_MptE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002826">
        <property type="entry name" value="MptE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036759">
        <property type="entry name" value="TPK_catalytic_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39648">
        <property type="entry name" value="6-HYDROXYMETHYL-7,8-DIHYDROPTERIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39648:SF1">
        <property type="entry name" value="6-HYDROXYMETHYL-7,8-DIHYDROPTERIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01973">
        <property type="entry name" value="MptE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63999">
        <property type="entry name" value="Thiamin pyrophosphokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="6-hydroxymethylpterin diphosphokinase MptE-like" evidence="6">
        <location>
            <begin position="39"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="244" mass="27412" checksum="ABDF7E82041EC1E4" modified="2018-07-18" version="1">MKLGWKKRYADILKEFNYSEKKDKESAILLNSILKESNANQKIIRLIEGKTVFVIGSGPSLLTAIPVLKRYRKSIKIAADSSLESLVKNGIMPNIIVTDLDGDEDTLKKIAKTQAIFVVHAHGDNMEKLALAKKFKNCIGTTQSKPFSKIQNFGGFTDGDRGVFLANHYGAKKIILFGMDFGKKIGKFSKTKKIDRETKLMKLKKGENLLEWLSTITKSELFTTSKSIKGFQKISYKELDIIIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTN6</accession>
    <name>A0A2S2KTN6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_17320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829111.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTN6"/>
    <dbReference type="GeneID" id="76208253"/>
    <dbReference type="OrthoDB" id="6171at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="48" mass="5469" checksum="D17B02E3A8758FFE" modified="2018-07-18" version="1">MEKFDAIKMLELVKVEDPDSDGDLTLIFQENKTLKIKVVDGKLVSEFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTR9</accession>
    <name>A0A2S2KTR9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11 12">Homoserine kinase</fullName>
            <shortName evidence="11">HK</shortName>
            <shortName evidence="11">HSK</shortName>
            <ecNumber evidence="3 11 12">2.7.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">thrB</name>
        <name evidence="15" type="ORF">NZNM25_18170</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-homoserine + ATP = O-phospho-L-homoserine + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.39"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the GHMP kinase family. Homoserine kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.39" evidence="3 11 12"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877618.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTR9"/>
    <dbReference type="GeneID" id="76210304"/>
    <dbReference type="OrthoDB" id="28273at2157"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00064"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004413">
        <property type="term" value="F:homoserine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00384">
        <property type="entry name" value="Homoser_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000870">
        <property type="entry name" value="Homoserine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002288">
        <property type="entry name" value="PRK01212.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00191">
        <property type="entry name" value="thrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF1">
        <property type="entry name" value="HOMOSERINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861">
        <property type="entry name" value="HOMOSERINE/4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000676">
        <property type="entry name" value="Homoser_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00958">
        <property type="entry name" value="HOMSERKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0791">Threonine biosynthesis</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="13">
        <location>
            <begin position="61"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="14">
        <location>
            <begin position="208"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="NCBIfam" id="TIGR00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GBH35026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="306" mass="32153" checksum="72C776577C819201" modified="2018-07-18" version="1">MESISIKAPSSTANLGPGFDVFGLAIDAFYDEITLTKTKGGITIITQDDIPTNPENNTAGLVVKNMKNKFKIKKGIEIKIKKGVPAGFGMGSSAASAAATAVAFDRLFGLGLDENTLVEFAGFGEKASAGTVHYDNVAASVLGGFVIVKTNPLEVIRIDPPINLRMCVAVPKLDVPKKKTKVSRGVIPKKVKLTDSILNISNASAIVAGFMKKDPELIGNSIKDVIVEPARQHMIPGYVKVKQNALKAGALGVTISGAGPSIIAFSQKSANLKKISLAMSKGFASAKIECQTIICKPSKGAVDKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTS5</accession>
    <name>A0A2S2KTS5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TIGR00374 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_18200</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877619.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTS5"/>
    <dbReference type="GeneID" id="76210301"/>
    <dbReference type="OrthoDB" id="8248at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022791">
        <property type="entry name" value="L-PG_synthase/AglD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00374">
        <property type="entry name" value="flippase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37693:SF1">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37693">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL LYSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03706">
        <property type="entry name" value="LPG_synthase_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH35029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="36959" checksum="BE45AE643A470360" modified="2018-07-18" version="1">MNWRLVAIPVTLIPIFIIAIQFDIQLDDLLAIGIFPFVGAIIVMMIKLGLQGIKFAYITRKYLGSFDSFFKLTGVRIGSEFIKFTTPMFVGAELVVIYYLHKKGVNPAKAAWIAIMDIVTEVFAAGLLSIMAGIIALLNGAYVVAAVILAVSIVITSLWMVMFFLSSKRTFQVPKILENLTKRFGKEKGAKAIKKTNTWMEEVCTMSRENLKTPESKKIFTISFLFSLASWSFYGISFMVIAMGTGFVISSFDSIMAVMGANAIGNLPITIGGSGLAEFGIVAYLNNLDPFAFEISEDIVGWNSVIGWRIATYYVPIVITWLLLVKLALSKISKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTW7</accession>
    <name>A0A2S2KTW7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_18850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877708.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTW7"/>
    <dbReference type="GeneID" id="76210112"/>
    <dbReference type="OrthoDB" id="2902at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="163" mass="17300" checksum="2984D8387A6CD734" modified="2018-07-18" version="1">MVSKLQLISNVLTLKRYAAIAIISGTGLGIIYYFLTMSMLPSHLDITMEIMPSYIITSVGLTITISALAGINFAMMAFKMKRMKMMNSVKGNSSSIVGGVFAAFTPGCPACTAPLAVVLGAIGGLSLFPMQGLELKFVSVGVLIFSIFWIARGLQSKSCCKIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTX8</accession>
    <name>A0A2S2KTX8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_18080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTX8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="208" mass="23627" checksum="964B76482411FE39" modified="2018-07-18" version="1">MLFASPLSVNYSFAESEDSQEEKVKVSKTIKKDRLWAILTEKVVDGKLELQRYALPANLSEEDLQRMPIKDQTSGWAYVNYKAFHSGIVLFDGKASKVGDNLWKISGKSELNLEERKFDLEISGKSNDSHDVIHEIVSDEDFSYKVIFSGKITETDEENEFAISFLISGLKNIETEQNIKLLQIGELSINSEESNSFTQEFRNSISVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTY1</accession>
    <name>A0A2S2KTY1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sugar kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_17160</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877532.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTY1"/>
    <dbReference type="GeneID" id="76208269"/>
    <dbReference type="OrthoDB" id="26949at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050306">
        <property type="entry name" value="PfkB_Carbo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43085:SF57">
        <property type="entry name" value="CARBOHYDRATE KINASE PFKB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43085">
        <property type="entry name" value="HEXOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="4">
        <location>
            <begin position="3"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="297" mass="33075" checksum="A71EF3CC6B943B91" modified="2018-07-18" version="1">MLTVFGSTALDTIRTPKKTLKNVLGGAATFAAISASNFVKTGLIAVVGKDFPKRHHDILSKRLDLQGFTIKEGKTFRYDGSYDNTLSTRSTLKTELNVLADFKPAVPEDYRKSQFVYLANNDPEQNTSLIKEFDKVKFSMCDTIDFWISTKRDAVIKMIKAVDAVVINDEEAKLLTKEFNLIKCAKKMMNWGAKYVIIKKGEHGSLMFYDDVIFPTAGFSLEDVVDPTGAGDSFAGAMIGYLASKKSTSMSEIKKAVVYGNVLGSFAVEKYGLDGLLKIKNEDISKRVKIYEKMIRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTZ8</accession>
    <name>A0A2S2KTZ8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_18280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877627.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTZ8"/>
    <dbReference type="GeneID" id="76210293"/>
    <dbReference type="OrthoDB" id="7396at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="221" mass="24418" checksum="03F22CD65A4910BD" modified="2018-07-18" version="1">MKYVFLILSLGFFFTPVFAQELTNPSLIINTIEIPAGEFNTILRDAPVIMLDDVHAVSWQVTIDNNLLYANPNGNAVLRLYDKETPDEFIEVGMGSQPDHKFWVAVQTPKEGYVVVHKDLDRGWYPQAKSIVSFTDTAGLTVNNGARIVVTNLDIGTFVIDSYSVYGMEDSTDPPAINSGVMIIEFLSGDPAKNTFAMLPFYMAAAIGIIVGILFLTKKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU08</accession>
    <name>A0A2S2KU08_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PEFG-CTERM sorting domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_17460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU08"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="22826" checksum="9150BA1051476783" modified="2018-07-18" version="1">MLLFLLITPSITGITYGHTVDSVGEYRVEIGWMNEPVVSGETNAIEFYVSPLEPGLELKDQVFKNGITDLKKSIKIKLIYKDESITLPLSPDHNIPGKYHAFINPTISGFYQANILGTIEDTPISLSMHPPKVEERAYIEFPEPSDLTLTQIIDGHTTLIGDINDLKESVSSLEESKQQMNVGYIGIGIGLIAIVIAILSLVKSKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU09</accession>
    <name>A0A2S2KU09_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_18380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877637.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU09"/>
    <dbReference type="GeneID" id="76210283"/>
    <dbReference type="OrthoDB" id="385464at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="66" mass="6811" checksum="F6A0EF183CFA8A3B" modified="2018-07-18" version="1">MNKVIIIGIIVAIIGIGALSVLGISSDSNNKESMDEFGNEVSSDDVKTEPKQFTIGLEESVGVSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU31</accession>
    <name>A0A2S2KU31_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_19300</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU31"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.280">
        <property type="entry name" value="Cobalamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051198">
        <property type="entry name" value="BchE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43409">
        <property type="entry name" value="ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43409:SF7">
        <property type="entry name" value="BLL1977 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="Cobalamin (vitamin B12)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="B12-binding" evidence="6">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="196"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH35139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="544" mass="61820" checksum="AF9A69D5D4692857" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU46</accession>
    <name>A0A2S2KU46_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NDP-hexose-3-ketoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_19650</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfo/Idh/MocA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877775.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU46"/>
    <dbReference type="GeneID" id="76210195"/>
    <dbReference type="OrthoDB" id="25239at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050984">
        <property type="entry name" value="Gfo/Idh/MocA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055170">
        <property type="entry name" value="GFO_IDH_MocA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22604">
        <property type="entry name" value="OXIDOREDUCTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22604:SF105">
        <property type="entry name" value="TRANS-1,2-DIHYDROBENZENE-1,2-DIOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22725">
        <property type="entry name" value="GFO_IDH_MocA_C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="3">
        <location>
            <begin position="3"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="GFO/IDH/MocA-like oxidoreductase" evidence="4">
        <location>
            <begin position="130"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH35174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="330" mass="37556" checksum="31335E63BF725C27" modified="2018-07-18" version="1">MTLKIGLIGCSRVAKNKIIPAVHFSDFISINAVGSRTNSKAKQYSKEIGCKTYGNYEDVIASDIDCVYISLPDGLHEKWAIKAAEAGLHILCEKSSTTSYESAKNIINACKLYNTRILECFSFRFHPQHKKVLQLIEKNELGKIYNFIGNFGYPKPDNENIRLKKELGGGILNDACCYPICASRIIFNEEPIGVMSNLIMDPIVDIDISASVFLMYKDKNAYAESGFDRNFKSNYEVWGSKGNIMLNRAYAVPNNFETQIYFTKNDNTETIKIDAVDQTFLMLKTFYEEIFKFKKSSFNFEEDLLKQAKILEAARLSSKKKKIIYLDEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU54</accession>
    <name>A0A2S2KU54_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ribonuclease H</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_18780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877701.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU54"/>
    <dbReference type="GeneID" id="76210105"/>
    <dbReference type="OrthoDB" id="52651at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13456">
        <property type="entry name" value="RVT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="118" mass="13174" checksum="476F0709DE9D37F7" modified="2018-07-18" version="1">MGISVYVDGSGGPNGGFGFFVKETGESFYEKKPEITNNQAEYMAIISALNKFVDSDEEIKIFSDSKNTVNQLNHEFAINNEQLRDLAREAWSVMGKFSNLSIQWVPRKENLAGKMLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU58</accession>
    <name>A0A2S2KU58_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytochrome c oxidase subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ctaD</name>
        <name evidence="3" type="ORF">NZNM25_19120</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877726.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU58"/>
    <dbReference type="GeneID" id="76210141"/>
    <dbReference type="OrthoDB" id="33297at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.210.10:FF:000012">
        <property type="entry name" value="Cytochrome c oxidase subunit I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="entry name" value="Cytochrome c oxidase-like, subunit I domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023616">
        <property type="entry name" value="Cyt_c_oxase-like_su1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422:SF18">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01165">
        <property type="entry name" value="CYCOXIDASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="Cytochrome c oxidase subunit I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50855">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="338"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="378"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="413"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="456"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome oxidase subunit I profile" evidence="2">
        <location>
            <begin position="6"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="508" mass="56182" checksum="A60FE9B51040FE10" modified="2018-07-18" version="1">MVLELQKPRPIWQIMFSTHHTDVGLLYLISSLAFLFLGGTLALAIRAELFLPGAQIIGDSMTFNRIFTVHGTTLIFLFIIPFASAVGNYYVPIMVRYKDMAYPKLNAIAFWMIPPAGALIWLGFADFTWYATPPYSIISAPGPAADMWIFGLKILGISSVLGAINFIVTILKCKHPDMSIGQVPLLAWSFLSSSLIILVAIPTFAAALLMLLTDRLGVSGFFNPAMGGDPIAYAHLFWFTFHPEVYVLVIPAIGMMYEIIPRFSRKPIYSYNSGVFAFVLLSIVGFSSWAHHMYATGMSFTEKTVFMVGTLAAVPASAMHVFNFIATMWNGRIKFATPMMWAVGGIALFFSAGAGGVANAAMPLDFTTHDTYWVVGHFHLFVMGTIAFGSIGFLYYMFPYVTGRMYNETMGKVHFVMSFIGTVLVFFTQHVLGLYGMPRRIFDYPPIPEWIAMNQIATVGAMIIGVSMAIFLANMIYSSGKGKLANTEDPFGVGGKYYYPFEAKNPSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU74</accession>
    <name>A0A2S2KU74_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NAD(P)-dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_19570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU74"/>
    <dbReference type="OrthoDB" id="4907at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05254">
        <property type="entry name" value="dTDP_HR_like_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005913">
        <property type="entry name" value="dTDP_dehydrorham_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029903">
        <property type="entry name" value="RmlD-like-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01214">
        <property type="entry name" value="rmlD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10491">
        <property type="entry name" value="DTDP-4-DEHYDRORHAMNOSE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10491:SF4">
        <property type="entry name" value="METHIONINE ADENOSYLTRANSFERASE 2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04321">
        <property type="entry name" value="RmlD_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RmlD-like substrate binding" evidence="1">
        <location>
            <begin position="3"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="296" mass="33592" checksum="CBF3D73E65D13CEA" modified="2018-07-18" version="1">MTKILVTGSTGLVGRQVVRDLVRKNYEVYACYNRIKPEFGIPVNLDLSKTDKIISVLHSINPNIIIHLAAITDLEKCEIQREQAVLINTDSTRILARESTKLGAFFIYVSTDYVFDGTEGYRKEDDITNPLNIYGKSKLDGEIAIRDSTSKYAIIRTSTPFGLHSTKKSFPLWIKENLEGGKETSVLIDQFTSPTYVPNLSKMIIEVTLKQIIGIIHLAGATRISRYEFAIMIAEKLDLDKSLLKPIKIQDMNWKATRPKDSSLDISKAKKILMNKPEEIEYALENFIYEIKNLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU82</accession>
    <name>A0A2S2KU82_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Adenosine 5'-phosphosulfate reductase</fullName>
            <ecNumber evidence="9">1.8.4.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">5'-adenylylsulfate reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Thioredoxin-dependent 5'-adenylylsulfate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">NZNM25_18260</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of sulfite from adenosine 5'-phosphosulfate (APS) using thioredoxin as an electron donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>[thioredoxin]-disulfide + sulfite + AMP + 2 H(+) = adenosine 5'-phosphosulfate + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:21976"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="1.8.4.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.10" evidence="9"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU82"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043866">
        <property type="term" value="F:adenylyl-sulfate reductase (thioredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004604">
        <property type="term" value="F:phosphoadenylyl-sulfate reductase (thioredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019344">
        <property type="term" value="P:cysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019379">
        <property type="term" value="P:sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23945">
        <property type="entry name" value="PAPS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00063">
        <property type="entry name" value="CysH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011798">
        <property type="entry name" value="APS_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004511">
        <property type="entry name" value="PAPS/APS_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02055">
        <property type="entry name" value="APS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00434">
        <property type="entry name" value="cysH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002537">
        <property type="entry name" value="PRK02090.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46482:SF9">
        <property type="entry name" value="5'-ADENYLYLSULFATE REDUCTASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46482">
        <property type="entry name" value="5'-ADENYLYLSULFATE REDUCTASE 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000857">
        <property type="entry name" value="PAPS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoadenosine phosphosulphate reductase" evidence="14">
        <location>
            <begin position="38"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GBH35035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="241" mass="28049" checksum="81173369D9CA1404" modified="2018-07-18" version="1">MKNVKQFTQEQVDELNSKINTTEEALQWVSDNLHPKVAKASSFGAEDAVVMDIMLKINPKFRFFTLDTGRLPQETYDIIDIVSKKYNISIEVLFPDTKEVEDMVREKGMNLFYESIENRKLCCEIRKVHPMNKMLKTLDGWITGLRREQTKNRENVTMFQLDHGHGGILKINPIINWTWEQIQEYIKKNDLPYNSLLDKGYPSIGCEPCTRAVKPGEDIRAGRWWWEQGGNKECGLHIDPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUG1</accession>
    <name>A0A2S2KUG1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Divalent-cation tolerance protein CutA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_19880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CutA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877795.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUG1"/>
    <dbReference type="GeneID" id="76210218"/>
    <dbReference type="OrthoDB" id="8015at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010038">
        <property type="term" value="P:response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004323">
        <property type="entry name" value="Ion_tolerance_CutA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23419">
        <property type="entry name" value="DIVALENT CATION TOLERANCE CUTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23419:SF8">
        <property type="entry name" value="FI09726P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03091">
        <property type="entry name" value="CutA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="103" mass="11689" checksum="B5A5C850192C2D71" modified="2018-07-18" version="1">MKPVIIVSTYPDKKSISKIANELVKNKTVACVNVSKISSIYSWKGKIENSSEYIAIFKTVTKNKKLLKKKILETHPYKVPEIAELTVSSLNKSYLNWLIESTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUM7</accession>
    <name>A0A2S2KUM7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">N-acetylneuraminate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_19760</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUM7"/>
    <dbReference type="OrthoDB" id="71219at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047444">
        <property type="term" value="F:N-acylneuraminate-9-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11615">
        <property type="entry name" value="SAF_NeuB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1210.10">
        <property type="entry name" value="Antifreeze-like/N-acetylneuraminic acid synthase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036732">
        <property type="entry name" value="AFP_Neu5c_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051690">
        <property type="entry name" value="PseI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013132">
        <property type="entry name" value="PseI/NeuA/B-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013974">
        <property type="entry name" value="SAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006190">
        <property type="entry name" value="SAF_AFP_Neu5Ac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057736">
        <property type="entry name" value="SAF_PseI/NeuA/NeuB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42966">
        <property type="entry name" value="N-ACETYLNEURAMINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42966:SF1">
        <property type="entry name" value="SIALIC ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03102">
        <property type="entry name" value="NeuB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08666">
        <property type="entry name" value="SAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00858">
        <property type="entry name" value="SAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51269">
        <property type="entry name" value="AFP III-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50844">
        <property type="entry name" value="AFP_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="272"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="AFP-like" evidence="2">
        <location>
            <begin position="286"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="343" mass="38402" checksum="13B1F70280AA5D1E" modified="2018-07-18" version="1">MKDIKIGNRIIGEHHPVFVIAEAGINHNGSLLIAKKLVDMAKKAGADCIKFQTHITEEEMTKTKILPGKISKKPLWDIIKSCELNVDEEKKLNEYCKKRKIIFLSTPFSIPAIDRLEKIKMPAYKIGSGELTNLPFLKAIAQTKKPVILSTGMSELSEIKETVKLFKKSKIPLAILQTTSEYPCDYKDVNLLVIDNYKKLFNLPVGISDHSLGIYTVLGAVARGACIVEKHITLDKKMPGPDQKISLEFNELKELVKGCRAIKLALGSTKKILKGELEVLNFARESVVTKEKISKGEIFTEANLITKRPNTGEIPAKNFYMILGKKAKKDIQKNKQLKFSDIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNT1</accession>
    <name>A0A2S2KNT1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Hydroxymethylglutaryl-CoA synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_00050</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. HMG-CoA synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875890.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNT1"/>
    <dbReference type="GeneID" id="76209330"/>
    <dbReference type="OrthoDB" id="5812at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004421">
        <property type="term" value="F:hydroxymethylglutaryl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00827">
        <property type="entry name" value="init_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013746">
        <property type="entry name" value="HMG_CoA_synt_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013528">
        <property type="entry name" value="HMG_CoA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323:SF2">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08540">
        <property type="entry name" value="HMG_CoA_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01154">
        <property type="entry name" value="HMG_CoA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase N-terminal" evidence="4">
        <location>
            <begin position="3"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase C-terminal" evidence="5">
        <location>
            <begin position="180"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="464" mass="52253" checksum="5D8A48C233EE6716" modified="2018-07-18" version="1">MAAGIDDIAIYIPRLYIDAADFAKARGLDPVKLQKGLGVAQMAIVDANQDPACLAANACLKIMQKNKLSPEDIGRLYVSTESAFDESKAMNSYVIGMLEQVYGQGAFEHCGGVETKFACVSGSYALYDNTNWIRAGEAEGKHALVVVSDIAKYDMGSSGEMTQGAGAVVMLLNDKPRLLAFDPKVTATSIKDEYDFYRPFGKETPIVHGQYSNLLYMIQVRKALESYKKKVISSGLIKIEPGETILDHMDYINMHLPYSNMGKKALAYLVRHEWRQLPRWKRILQEIGIEEPRPKDPRGTIESVLADEEFMAKDHEFTKLFTKTHEYQEVYESKLSSSLIASSMIGNLYTASLYLGFRSSLEYEFQKGVDLEGKRIGFGSYGSGSSAMVFSGVIQPEFKEIVRDMNLEAEIGDRKKLTWEEYEELHENKLLPEESMVHSKKEFVLVNVKTDTESRGERRYIFNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNV0</accession>
    <name>A0A2S2KNV0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CdvA-like coiled-coil domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_01440</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876013.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNV0"/>
    <dbReference type="GeneID" id="76209474"/>
    <dbReference type="OrthoDB" id="12104at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041461">
        <property type="entry name" value="CdvA_CC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18822">
        <property type="entry name" value="CdvA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CdvA-like coiled-coil" evidence="2">
        <location>
            <begin position="84"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="299" mass="33487" checksum="82E515F708EC3A0E" modified="2018-07-18" version="1">MTHDDIEIIGKKVKDMYGTFMGKVVGTITDIDGSIQSVGIDCGSQGLQQIQYEQLVVQGDVVIFIPKWRLDSQRLIREKQLTIRRLKALIDIVSENDDMKVDAEIIHEKYKSKLASLDETEHEIKAKLEARLTELDEQMKSAKMLSFDAKVQFKSNEISDATFETVKACTTEIIEHVSHETAEIANVKSRIADLELEVQEITSPSTPDIQESAVSYLETSEPQQVVQSILPEAPTEPIVTHSEKFASHSEPIEAGVLPIPEPPTNSETTFAFPEPPQQVTPETPKDDNDNDWLARMEAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNX1</accession>
    <name>A0A2S2KNX1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exonuclease SbcC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_01270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876000.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNX1"/>
    <dbReference type="GeneID" id="76209456"/>
    <dbReference type="OrthoDB" id="12116at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="172"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="424" mass="48629" checksum="098043094300E441" modified="2018-07-18" version="1">MVFGWGKKKQEAAKEAPNDKTINLSDVPQIVTELRQLRQSQALSDIKRLRDATVPLIDDLTNIGKVLEKDNLNVDDIDKHLAIIVVRGKKQVIDIIKKGVTGIPKISSIDDAQELDSTLNQILKKVGDVLGRQTRVIHIFAKKYAEQLKNNLEVMNKNHYEIHDILKNFDSMKIISDEIMNVLSEIKNLQETRMQNEKKIVEITSHLSSIDEKISSIQSSIEEIKSSDNYQKYVELKKSLDEFETQKIKIKNDIDAQFTKISRPLNRYEYASSLDKDQKNLLAKLNTDPFDVLTPKNKDSIIVILENVRKAVSSSSISVKDVDKTLAQITETEEALDGFLKQVLAYFQKHEQLENDLNSLKPKSLISLEDDLEKNISFKEDYEIKSKTIKNEIDEIRLKIPQFVTEIEDKLRKFSNTKYVLDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNZ1</accession>
    <name>A0A2S2KNZ1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Histidine kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_01940</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876062.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNZ1"/>
    <dbReference type="GeneID" id="76209526"/>
    <dbReference type="OrthoDB" id="8127at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065:SF10">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065">
        <property type="entry name" value="SENSOR HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="49"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="257" mass="28792" checksum="A3518C20235DFAF2" modified="2018-07-18" version="1">MHDNSTILTNILNLDQINTIKELKKIIDLQAKEMIKQKKMVQIGEIASRLSHDLRNPLSVIMVSVENLAMLYGTDNQKQKHYDRICHSIDRIVNQVNNVLNYVKDQPTVLSKTKTSRIILDSMDSILIPNNVKINISKNDYDVVCDREQLSIVINNLVVNAIQAVGVKGSIDIRVGKERNWIVFEIEDSGNGISEENLPYIFKPLFTTKKTGTGFGLVSVKSVVESHGGEITVSNSPTIFKVKIPKTPPGNFSSNYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP01</accession>
    <name>A0A2S2KP01_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_02040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876072.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP01"/>
    <dbReference type="GeneID" id="76209538"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="90" mass="10137" checksum="65AFD4C77DCDAB4A" modified="2018-07-18" version="1">MLKILVGVLVVVSLFFMPTSIYGHPEINYTKSFVKFSDDTHMHLKQPEIKRPTLGEEDCDWLNQCIIITLIGIIGVAMITVVVVVYKKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP66</accession>
    <name>A0A2S2KP66_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Roadblock/LAMTOR2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00760</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875953.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP66"/>
    <dbReference type="GeneID" id="76209405"/>
    <dbReference type="OrthoDB" id="4782at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046600">
        <property type="entry name" value="DUF6659"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20364">
        <property type="entry name" value="DUF6659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14939" checksum="20F073A1B6E4FE2A" modified="2018-07-18" version="1">MATATKFDYLEIVQKILNLDPKMRFAAIIDPKGNIREAIMKTGKTSLKTQKEEEHFCKQVAQRRSMRKEFDRSLGKVRYVHVEREKVSQMVIYTKRNIVYFTMEPEMPIDTKIRLITRIKKITSEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP68</accession>
    <name>A0A2S2KP68_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Inosine-5-monophosphate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_02200</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876087.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP68"/>
    <dbReference type="GeneID" id="76209554"/>
    <dbReference type="OrthoDB" id="8919at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="11"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="76"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="141"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="233"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="284" mass="31825" checksum="30F206744B854EE8" modified="2018-07-18" version="1">MDVKNIKLSELITKPITVNPNSTLLKTRESLLKNKVKRVVIVDKKIPVGVITEKDIAKKIYELGSKPIKSVKAKDFIPKKLFTLTRDNSVQECAKLMKKHRISLVIITNKDNTLGGIVTETDLVKAFLTKESTSFKVSKIMKKELITAAPSDPILHVESLLLKYGISRVIIKRNQIPVGIITFRDFVPAKIPQWIAESADPKEVQEYKFKKGLEEIHSNQMSYLFPFHATDIMTVKPITIDMDEDIKTAITLMVKHNISGLPVVKKSKLVGIVTKSDVVAALAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP80</accession>
    <name>A0A2S2KP80_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DoxX family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_01680</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876036.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP80"/>
    <dbReference type="GeneID" id="76209498"/>
    <dbReference type="OrthoDB" id="10826at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032808">
        <property type="entry name" value="DoxX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051907">
        <property type="entry name" value="DoxX-like_oxidoreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33452:SF1">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33452">
        <property type="entry name" value="OXIDOREDUCTASE CATD-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07681">
        <property type="entry name" value="DoxX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="14581" checksum="74029619B34BAD8B" modified="2018-07-18" version="1">MTTAEIREKILNDVVFMGLRSAVGVIFILHGMSKFNPGFANNLPNMGLPIEMQIPLALAELVPGILIIVGVLSRLSASLLAVVMLGAIFMIKGAKSITGQGGVELDLILLASVLVVMIVGPGRISIAQAIKKLPRCLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP83</accession>
    <name>A0A2S2KP83_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875946.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP83"/>
    <dbReference type="GeneID" id="76209398"/>
    <dbReference type="OrthoDB" id="7605at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="83" mass="9955" checksum="7B0416CAB4B2CD5A" modified="2018-07-18" version="1">MDSCDRRIRAYKNGKTFDQCKEIAESMNPDFKKYIETNGRVLWTEILEKVDHDELLYKLTLKFLRRDGYDIGNHKVPEVKKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP90</accession>
    <name>A0A2S2KP90_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF504 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01780</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876046.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP90"/>
    <dbReference type="OrthoDB" id="8488at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="70" mass="8314" checksum="E4384DF6FA68D98C" modified="2018-07-18" version="1">MVKKGTIEEIFSKALFADEPKEYRISYRDFQRIKETSLPEFLVRSNNFQTIPISRIKSIKKSNTILFEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPC6</accession>
    <name>A0A2S2KPC6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Oligopeptide ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_02350</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876098.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPC6"/>
    <dbReference type="GeneID" id="76209569"/>
    <dbReference type="OrthoDB" id="18209at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000016">
        <property type="entry name" value="Oligopeptide ABC transporter ATP-binding component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050319">
        <property type="entry name" value="ABC_transp_ATP-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43776:SF8">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43776">
        <property type="entry name" value="TRANSPORT ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="321" mass="36735" checksum="6DDC5ACBBC3ED006" modified="2018-07-18" version="1">MKEILKVEHLQKYFNKKSMFGGKISTVRATDDVSFSLEIGEVLVLAGESGSGKSTIAKLILRSIQPDSGKIFFEGHEIDDEKKNLEKIRMNCQMIHQDPYDSINPRMKIGDIVSEPLEIHNIGNKQQRLKRVVEVLQEVKLEPANEIIKKYPHMLSGGQRQRVVLARALSIKPKIIIADEPVSMLDVSIRAEMLELMHDLQKKYNISFIYITHDLATAKYFGQRIGVLYKGKIVEMGPIDQVLLKPRHPYTQALIDAISEPDPDNLKREKKIRIKDATDEDVFQGCRFRARCPYVIEKCAEEPELLEISDRHYSACHVKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPC7</accession>
    <name>A0A2S2KPC7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase M10 metallopeptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876007.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPC7"/>
    <dbReference type="GeneID" id="76209465"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10462">
        <property type="entry name" value="Peptidase_M66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="67" mass="7443" checksum="A30C05053A8A47CF" modified="2018-07-18" version="1">MLAENSGASPQEFDVRKTLGHELWHAMGMDHDNGNTANLSYYVYVFGATNGYEITSAEATNLENQYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPF3</accession>
    <name>A0A2S2KPF3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Preprotein translocase subunit SecG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">Protein transport protein Sec61 subunit beta homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">secG</name>
        <name evidence="14" type="ORF">NZNM25_02650</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in protein export. The function of the beta subunit is unknown, but it may be involved in stabilization of the trimeric complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the protein translocase complex. Heterotrimer consisting of alpha (SecY), beta (SecG) and gamma (SecE) subunits. Can form oligomers of the heterotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the SEC61-beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876267.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPF3"/>
    <dbReference type="GeneID" id="76209600"/>
    <dbReference type="OrthoDB" id="43651at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00751">
        <property type="entry name" value="SecG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023531">
        <property type="entry name" value="Preprot_translocase_SecG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016482">
        <property type="entry name" value="SecG/Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002318">
        <property type="entry name" value="PRK01253.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03911">
        <property type="entry name" value="Sec61_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="6 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="12">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GBH33474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="56" mass="6024" checksum="C377CD567F41D431" modified="2018-07-18" version="1">MSSNKKKSAPLPASSGGLMRFFEDETKGFKIDPKIVVSVPIGLIVVSWAIDLFLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPF7</accession>
    <name>A0A2S2KPF7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876185.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPF7"/>
    <dbReference type="GeneID" id="76209676"/>
    <dbReference type="OrthoDB" id="2856at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="143" mass="16640" checksum="E602D1FBDD030A7B" modified="2018-07-18" version="1">MTSNDEITQMFDTESEKLYNLINNATKINLPIHEIIETYYQIINVSSMITMLKEKINSGNASLLDKIHETEKLVSEKFNSDIHPKIMDYLKICIQQDTQSLQLGNREKSKEETESEAKLFEELRQKMSTKEFVEQYDQGLLNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPF8</accession>
    <name>A0A2S2KPF8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_02020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPF8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="309"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="415" mass="48306" checksum="1B7614D316615F13" modified="2018-07-18" version="1">MLAFVALVFINTAYAQITNEQVTLSGDLQNNPVAQDILDKIEKSKKWIAKLQERNFEVSERQRELEEKRAEVLEHLNADLKEWEDLWEYYTFDKMLERALEDSPAKHTDTIYDHPLKFTASKINAGRAALAQVIQNGGGPEEARDAFVNAAQITRVEMVAANALFNVLNGNAYYNQQILFESDGQFNLDLSGEQLRKYYLDYRTNPGYLLANPKDTTSWEDLSETNPDTVCRDEYILIYRNHADDYVCVTEYTAEMWQRHGMGNPVHKETSSIPDELTKIKLNHDRIAEKVSAINLKIQKIHDNHEIQIQNMEKKYDHMFVQMEQEQKDEEKLVLNKLNSDEIDAENFSVQITDIREKYKAVEKNMIKEKFQTLDILDKSLRENLDTLMSKYEHDSEIKIVWNDTQGTYRATMNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPK1</accession>
    <name>A0A2S2KPK1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sodium:calcium antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">yrbG_1</name>
        <name evidence="7" type="ORF">NZNM25_02880</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876143.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPK1"/>
    <dbReference type="GeneID" id="76209625"/>
    <dbReference type="OrthoDB" id="142185at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008273">
        <property type="term" value="F:calcium, potassium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.80">
        <property type="entry name" value="NCX, peripheral helical region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004481">
        <property type="entry name" value="K/Na/Ca-exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00367">
        <property type="entry name" value="calcium/sodium antiporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846:SF8">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YRBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846">
        <property type="entry name" value="SODIUM/POTASSIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="37"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="267"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="291"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="6">
        <location>
            <begin position="7"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="6">
        <location>
            <begin position="170"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="311" mass="32758" checksum="195E1C255E944F7C" modified="2018-07-18" version="1">MEIVINAVLTIVGLAMLCFGGNWLVSGGVTIARKFRISNLVIGMTIVAYGTSTPELAASVAAAGEHSAIILGNIVGSNIANVGMVIGIAAILVPLAVSKSVLRKEIPIMLGVSFLLILISIDGELSIYDGILLLVGLGVYGYYTFKDAMKHREESKENVEESKNNVYLKSFGLIGIGVVILYFGAILTVDNAVILAQEFGLSEKIIGLTVIAIGTSLPELITSIIAIRKGHGDIGVGNIIGSNIYNILMIMGVGAALGGVMVAPDVFIDYAIMIIFSISLLIALKTGIINRVMGACLALGYVVYLIFTFFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPL3</accession>
    <name>A0A2S2KPL3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase M10 metallopeptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_01990</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPL3"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M10 metallopeptidase" evidence="6">
        <location>
            <begin position="225"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="291" mass="33116" checksum="6AD3ECF1BFCC5ED0" modified="2018-07-18" version="1">MVYVHLDKQNGDSAAMGEKLDQISNQIGLINQKYEKLDSVLHRNTSCFFVLFCSIIVVFFVIIFSISHSNIQPLESGHFITQSLIGDSIDTWLSWKLSDSERIFHVHIINHAKTSQKNIDLIKESIMSEKIIEINDLDTHKGTSKNSSKFYKGWQGVLNEFDSLELRYNIPIRLHVHESTSDDGDILILLVDEKNSEGYSGYTRSIIDEDKNQILKSRITIYESSKLDGNRLAAITRHEMGHALGLQHSTDPDDLMYYKITTTNPFISECDVSALKSLYGGETMSKFLCEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPM0</accession>
    <name>A0A2S2KPM0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_03350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876183.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPM0"/>
    <dbReference type="GeneID" id="76209673"/>
    <dbReference type="OrthoDB" id="117061at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043713">
        <property type="entry name" value="DUF5654"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18898">
        <property type="entry name" value="DUF5654"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="121" mass="13159" checksum="ED593EDF355B11D0" modified="2018-07-18" version="1">MTDDEGPKSIKFEILDKIAALIAAAFGLVAALAWNDAIKALFKEIFGTTDQLGPMIGYAVVVTVIAVILTIFIARAASKAKSIITRTYSCSLCDFKSEVQSEFMEHVTKKHAANDDKFLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPQ6</accession>
    <name>A0A2S2KPQ6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cation/H+ exchanger transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_04510</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876322.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPQ6"/>
    <dbReference type="GeneID" id="76209803"/>
    <dbReference type="OrthoDB" id="43518at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42751">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER FAMILY/TRKA DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42751:SF3">
        <property type="entry name" value="SODIUM_GLUTAMATE SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="197"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="220"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="268"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="8">
        <location>
            <begin position="49"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="442" mass="46730" checksum="4ECE232126EFEF8C" modified="2018-07-18" version="1">MQNISLQIDTSEIHSSLNTTVTSLIEQITPDLPHTTFVTDLAFIMIIGAVVTLAFFKIRQPLIIGYLFAGMLIGPLSPFWSWVLPEGGPSSDSSGGVGILSDISALNLFSEIGVILLLFVIGIEFPYAKIKSIGRVAVGVGTMGLFSTLGVVFYTATALGMEFLDALFIAAALSISSTAIIVKILEEMGRIKKESSILVLGILIVEDVIAVILISSLQSIALVGTVSIESVIVVVLVAVGLIVGTFTIGTRIIPPLIDRVAEAEHREILLLSILGVCFGYALLANVVGLSVAIGAFLAGVLIAESKSSEVAKLLSSPIKDMFVAIFFISVGALMDISQLENHIFLAIALIAVAIGMKFGGNLIGNLVFRQKRSKAIRSAFTLAAPRGEFSIVIVKVGVDIGAVSAFLFPLMGIISIITAFISPFLVKAGDKIIPTLKEKQDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPV4</accession>
    <name>A0A2S2KPV4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TIGR00289 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_03680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876214.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPV4"/>
    <dbReference type="GeneID" id="76209709"/>
    <dbReference type="OrthoDB" id="372052at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017178">
        <property type="term" value="F:diphthine-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01994">
        <property type="entry name" value="AANH_PF0828-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1490.10">
        <property type="entry name" value="putative n-type atp pyrophosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002761">
        <property type="entry name" value="Diphthami_syn_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030662">
        <property type="entry name" value="DPH6/MJ0570"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022427">
        <property type="entry name" value="MJ0570_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03679">
        <property type="entry name" value="arCOG00187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00290">
        <property type="entry name" value="MJ0570_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196:SF2">
        <property type="entry name" value="DIPHTHINE--AMMONIA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196">
        <property type="entry name" value="DOMAIN OF UNKNOWN FUNCTION 71 DUF71 -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01902">
        <property type="entry name" value="Diphthami_syn_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039123">
        <property type="entry name" value="Diphthamide_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Diphthamide synthase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="229" mass="25795" checksum="B65E92EFB2CAAFB6" modified="2018-07-18" version="1">MKLASLFSGGKDSTFAIHLAQKQGHIVSCLLSIFTKSDESHLLHHPTLQWTKLQSETMKIPQLTMASDSDDTDNELLLLEKLLQKAIDEYKIEGLVHGGIKSNFQKEKFETLCTKLNLIPFTPLWGTDPENYMNDLVDSNFEFIITSVSSDGLDDAWLGKSISKSDVDTLRNLSEKFGFNLNFEGGEAETFVVNCPLFSNPIIIYESKKEWDGYRGRFEIVDAGLNYNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPX6</accession>
    <name>A0A2S2KPX6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_03160</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876169.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPX6"/>
    <dbReference type="GeneID" id="76209653"/>
    <dbReference type="OrthoDB" id="3152at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="220"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="261" mass="30465" checksum="4FA5EDF66EE355D5" modified="2018-07-18" version="1">MGLFGKKKEEAETVSQNSEEYVLKEELEAEVEKLQEEFRAKQGQLDEITQKIKSVKEEYDATVGNLMLVKKELNQKKMEHDIIQRECKEMEARIKNAEKIKDSKSIEEFKQTEENLAKLKRELEEFTEKQKELKEEIEQSKSDLHNIRKQQIDTQKELDEANSRLYNAKEELNRKDQFQDIDVLTPKEKQFITGEKSNEKSSAGIIEAASAVVGSLKSKLSMTEKELETVQLLLEKEREEHQVTKKELDEIKTLSKKLEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPZ0</accession>
    <name>A0A2S2KPZ0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829024.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPZ0"/>
    <dbReference type="GeneID" id="76209657"/>
    <dbReference type="OrthoDB" id="54at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="57" mass="6444" checksum="231BDEC896601973" modified="2018-07-18" version="1">MVGPQDGGFGFDQSKKYTTVENIEEVCVQCQAGQHKKCLKKSKEQEICECEHCMIYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ02</accession>
    <name>A0A2S2KQ02_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">YkgJ family cysteine cluster protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_05610</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ02"/>
    <dbReference type="OrthoDB" id="36424at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005358">
        <property type="entry name" value="Puta_zinc/iron-chelating_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35866">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35866:SF2">
        <property type="entry name" value="YKGJ FAMILY CYSTEINE CLUSTER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03692">
        <property type="entry name" value="CxxCxxCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="235" mass="27039" checksum="50137F10EF6CC600" modified="2018-07-18" version="1">MSSQNEIEESLNLLEKDWDVDPILRKFMLGKITDVSDYPLKVKDVIFHVPYLNSEKKFILWKCFWPDCHNCCDRQGRLPLTSDDLITIGKGLKYQKPSDFIKNETLTVTYQEPGPSGQLTTMTTINLKRKTDETPKDDGTHISCRFLDEKGGCSMHPDRPGVCYLYPFSSWLENENGIARVHATYQFTGDCPGFYLAETLDPMKEEFKDYSKTIYDYNMASNRTNREGFGSVSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ26</accession>
    <name>A0A2S2KQ26_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04120</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876291.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ26"/>
    <dbReference type="GeneID" id="76209760"/>
    <dbReference type="OrthoDB" id="11047at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="100" mass="11375" checksum="E67A390BBBDBED6F" modified="2018-07-18" version="1">MKLYNVKLLTIVCEILAQKNILEILKNHEITGYTTYEVDGNGARGLRGQGFKNEKNVKVEVIMHEEKLSDVVEEISRTLFANFAIVLYVSDVGVVRTEKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ34</accession>
    <name>A0A2S2KQ34_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rps4</name>
        <name evidence="11" type="ORF">NZNM25_05910</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="6">With S5 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit. Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876442.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ34"/>
    <dbReference type="GeneID" id="76209943"/>
    <dbReference type="OrthoDB" id="10429at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01306_A">
        <property type="entry name" value="Ribosomal_uS4_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022802">
        <property type="entry name" value="Ribosomal_uS4_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005710">
        <property type="entry name" value="Ribosomal_uS4_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003139">
        <property type="entry name" value="PRK04051.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01018">
        <property type="entry name" value="uS4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF5">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 11" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS4 N-terminal" evidence="10">
        <location>
            <begin position="5"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="RNA-binding S4" evidence="9">
        <location>
            <begin position="103"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="157"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="159"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="174"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH33800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="198" mass="22829" checksum="D36DC7DF85AE2AA0" modified="2018-07-18" version="1">MGDPKYPRRVWRKPKRPLNYELKMEELKTLGTFGLRTKRELWKAHTELSRVRHQARSLLALRQEIREEKEPILMKSLARIGLVSSDATLDDVLNLNANDLLSRRLQTIVTKKLGFKTPYQARQAVIHGHIMIGDRKVDIPSYTVTVEEENNIHFSPESKIPEMLEKTKSKEPVLDTDVEETAETAVEEPIKDESSSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ89</accession>
    <name>A0A2S2KQ89_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable transcription termination protein NusA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nusA</name>
        <name evidence="8" type="ORF">NZNM25_05650</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Participates in transcription termination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NusA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876421.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ89"/>
    <dbReference type="GeneID" id="76209917"/>
    <dbReference type="OrthoDB" id="4116at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22530">
        <property type="entry name" value="KH-II_NusA_arch_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22531">
        <property type="entry name" value="KH-II_NusA_arch_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.20:FF:000024">
        <property type="entry name" value="Probable transcription termination protein NusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.20:FF:000039">
        <property type="entry name" value="Probable transcription termination protein NusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00945_A">
        <property type="entry name" value="NusA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010212">
        <property type="entry name" value="NusA_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030842">
        <property type="entry name" value="TF_NusA_bacterial"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01952">
        <property type="entry name" value="nusA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22648">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR NUSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22648:SF0">
        <property type="entry name" value="TRANSCRIPTION TERMINATION_ANTITERMINATION PROTEIN NUSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 6" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="KH type-2" evidence="7">
        <location>
            <begin position="16"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="KH type-2" evidence="7">
        <location>
            <begin position="86"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="154" mass="17201" checksum="45060392A2045B67" modified="2018-07-18" version="1">MTQSIKLTTDQMRMMSLFQNVTGATARDCVEDEKQDRVIFVVNTGKMGLAIGKGGVHIKSLQNIVKRNVELVEYDEDPAKFLSSLLNSKLISEVKINTRADGTKQAIVMVDPRKKGIVVGREGRNAEKARLLAKRYFDITSVLINSPERATMEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ90</accession>
    <name>A0A2S2KQ90_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Intracellular proteinase inhibitor BsuPI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_05080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876370.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ90"/>
    <dbReference type="GeneID" id="76209859"/>
    <dbReference type="OrthoDB" id="24807at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="16075" checksum="D409228D2203A749" modified="2018-07-18" version="1">MLAKSLVIFIVIGVVSGFAYGVYLMDVKNTSQLVFVDGPSISLVTEKFDFKKGESIKIRVVNSGTTPLTFSDTSYGLRITGLSGILMYTPVGVPVISNLEPGDEIVLYWDQIRNDGNVALEGLYKISAKGTDEKGNSVEKSTTVTIWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ96</accession>
    <name>A0A2S2KQ96_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_06200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876469.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ96"/>
    <dbReference type="GeneID" id="76209975"/>
    <dbReference type="OrthoDB" id="6815at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="72" mass="7760" checksum="0BC0F6A4BC27ADB1" modified="2018-07-18" version="1">MDKRKGTGITIFVLCIGGFFLYAYLLMLSEWSPIVLQLSVLMIAGGILGVIAWIGYVMATTKPSSASITSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQA7</accession>
    <name>A0A2S2KQA7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQA7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="169" mass="19984" checksum="69A46683BB124E52" modified="2018-07-18" version="1">MIIIGILFKKIMIFSEIVTDLQNQLKKDLAQIRFLLKKNPALGYARIVEIGKEVGKKYNIKLIVNFPKKGMIDEFDMYGKRDLSLIVDYDLKRFPIDREIIKQKAKEILGDVQTEDAYMYENKEGVRVFTDNWKIDILPHSVHIWTEFNENVTAFCNWLMENAYKMKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQB2</accession>
    <name>A0A2S2KQB2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_06530</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_264953678.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQB2"/>
    <dbReference type="GeneID" id="76210011"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="52" mass="6348" checksum="8E9940046C892D79" modified="2018-07-18" version="1">MTDEIIKNIEKFGLKINHMLNHMNKKELENINQAIIEYQKNLQNSDYIDYMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQB6</accession>
    <name>A0A2S2KQB6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Biotin carboxylase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">accC1</name>
        <name evidence="8" type="ORF">NZNM25_06400</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876485.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQB6"/>
    <dbReference type="GeneID" id="76209997"/>
    <dbReference type="OrthoDB" id="31083at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000003">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000010">
        <property type="entry name" value="Propionyl-CoA carboxylase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45007">
        <property type="entry name" value="CARBOXYLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G07570)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45007:SF1">
        <property type="entry name" value="CARBOXYLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G07570)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="7">
        <location>
            <begin position="1"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="6">
        <location>
            <begin position="120"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="495" mass="55004" checksum="8C5244AD6B3C06F6" modified="2018-07-18" version="1">MIEKVLIANRGEIALRVIRTCKALGIKTVAVYSDEDYNSMHVKQASEAYHIGEAAPAKSYLNQEKILEVMLSAGADAVHPGYGFLSENDDFARLCEKNKINFIGPSADSMNLCGDKMRCKAAMLKAKVPTVPGSPDLVKDVEEAVSIANDIGYPVLLKSVYGGGGRGIRLANNDKELREGFVSVTGESIAAVGKSAILVEKFLEKTRHIEYQMARDKHGNTVHIFERECSIQRRNQKLIEQTPSPVVDEEIRERIGEIVCNAAKAVDYTNLGTAEFLRADNGDFYFIEINARLQVEHPITEYVSGLDLVKLQLDIANGEEIPFKQSDLKMNGYAIECRINAEDTFLDFAPSTGPVPNVTIPAGPGVRCDTYLYPGCTVSPFYDSLMAKLVTWGQTFEESRTRMLAALNDFYIQGVETSIPLYKTILSSEEYKNGQLSTDFLKRFDMIDRLTEDLKNEKIDKSEAAIAAAVIHSEYLKSRIQNDVSASSNWKYKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQC6</accession>
    <name>A0A2S2KQC6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04490</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876320.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQC6"/>
    <dbReference type="GeneID" id="76209800"/>
    <dbReference type="OrthoDB" id="12053at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="87" mass="10313" checksum="5A7693B2EF6444E4" modified="2018-07-18" version="1">MDEILLGKIEQKIRETISNKDEIKEIIQLLSNIDDSKSFALGVVVGRIYNAFYYQSKRILNREPTKDEFQEFLKFVKNNKSDLENLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQG5</accession>
    <name>A0A2S2KQG5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ArsR family transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_05880</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876440.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQG5"/>
    <dbReference type="GeneID" id="76209940"/>
    <dbReference type="OrthoDB" id="9514at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="103" mass="11552" checksum="ABAE7037CB37FE38" modified="2018-07-18" version="1">MESAFDKEKVVDCMFDPVTSSILAELEDGEKECSFLAKQSSISENDVLERLSYLIEHSFILQNFDGENCILSANAEKLNSIVENSDNFDATINGLEKMDSYLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQI8</accession>
    <name>A0A2S2KQI8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_07410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876602.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQI8"/>
    <dbReference type="GeneID" id="76208935"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="74" mass="8769" checksum="5EE95BAB77EE27D8" modified="2018-07-18" version="1">MDDPYLDELKNDFRDYSNQLKKLKKKLLKTNSIESQSKIIQQIDSIANKMEKNQKQSVKVIKSRLKQTKKKSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQK2</accession>
    <name>A0A2S2KQK2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07430</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQK2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="77" mass="8900" checksum="4EB77B53042722B6" modified="2018-07-18" version="1">MMDDELPESLKKYLDVLDENVDILANLEFDLGDELEDELTPHMGLHNLYDMTEDVAQSARLKRAESNFSRRQAEDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQN0</accession>
    <name>A0A2S2KQN0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF5615 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876617.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQN0"/>
    <dbReference type="GeneID" id="76208919"/>
    <dbReference type="OrthoDB" id="12308at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="102" mass="11549" checksum="F3FEE343536AD86B" modified="2018-07-18" version="1">MTRKVVIDEDIHGWSEENHDMLRPYKSIIKVGEDSESLKRGSSDEIVADYCNANGCDLFTSDVGFYAKCIKAGIKTIQITNCGFWEKGKKQIFLVKIIESLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQQ2</accession>
    <name>A0A2S2KQQ2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_06780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876516.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQQ2"/>
    <dbReference type="GeneID" id="76210038"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="76" mass="8425" checksum="82B25D46AAAE3092" modified="2018-07-18" version="1">MKSDLFGFLFAMWILILLGGGILVTILGPISITGYGEFSLFIASIIKAIVAIVLVVVWILILSKLKNWIFKKEIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQV6</accession>
    <name>A0A2S2KQV6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_160049206.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQV6"/>
    <dbReference type="GeneID" id="76208836"/>
    <dbReference type="OrthoDB" id="2985at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="51" mass="5981" checksum="24D80FF4F0BFAE69" modified="2018-07-18" version="1">MLVETIQTEDLSQLDKLINEIIEQRTVMDIKLSVTPFREKLVYTALIMLDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQY1</accession>
    <name>A0A2S2KQY1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07580</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQY1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="467" mass="56187" checksum="A2526EA21E1489B3" modified="2018-07-18" version="1">MIFARLYKWYGLEFFKEKYQPSALELIKIRKNFQKFNWRNSHQKYLLTIVKILAEHGPSTISEIVEKDGFPQEKKRKLTRHKIYSRIIEGSKDGKVKGLLPHNILHVAEKVLTSKPSKKYELSYYGIFLAIRVFAQDDFSLVNNMEKIGRDRFDEVQKQFITDNMIFLENLAKNYSIFLPLIFGKWEFIKNKFPDGADYLIKFAKEGYYEQDQIEYDEPFMNSTIIEDLYPENSSFHFFPEHFDWKEGNFFQEEITMWFYTLLLKSHQEYDFQQNLRIDQEVLTWFTNHTNRVIARQKELLSNAKNIKRWLQKNKSEKIEEFLQIALDGMSSNYWKKDHKLSQLQKNILKIENEIDKITITENGMGRILDMEKYKKLIDEIEPLRMNLRDKTRKKIIKIMENRKFSKKLVIEYLSEIHYILESKYSERLFTISLGGGKKPGWGDIPIRQVPPEELRRWIRRNNWDVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQY4</accession>
    <name>A0A2S2KQY4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876594.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQY4"/>
    <dbReference type="GeneID" id="76208943"/>
    <dbReference type="OrthoDB" id="3307at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="192" mass="22487" checksum="320108477701CF5B" modified="2018-07-18" version="1">MNKSLILLPLIAIPIIFILISPQDSKKYSELDLTGQDAILKVGEDFIKQASVEPLDQDHILNELREITEEEIRKETQIHDPTESEKIFTNGMKLSDDYNRISFEYSTEKLNVTEFFSKLLDFKIKYEKYMTAIDSYIGDEDILILKNSMVKEYEKINEQITLIKNSGTIDDEWESKTEFDKYKQYLPSMFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR07</accession>
    <name>A0A2S2KR07_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_06790</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876517.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR07"/>
    <dbReference type="GeneID" id="76210039"/>
    <dbReference type="OrthoDB" id="34534at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044038">
        <property type="term" value="P:cell wall macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06856">
        <property type="entry name" value="GT_GPT_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926">
        <property type="entry name" value="PHOSPHO-N-ACETYLMURAMOYL-PENTAPEPTIDE-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926:SF3">
        <property type="entry name" value="UNDECAPRENYL-PHOSPHATE ALPHA-N-ACETYLGLUCOSAMINYL 1-PHOSPHATE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="307"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="330" mass="35665" checksum="D28F4D1C1A9680ED" modified="2018-07-18" version="1">MIELILPVIVSCFIAFFLVFVMTPPLIKFLEKRNLTVKDMNKKEDVMVARPGGISIIVGIIASEITLYVFLQANEILAITITTFAAFLIGYVDDRRVMGGWFKPIALGIAAIPIIVFGAYDSDLAFPLFGTVQIPALYLGLIIFMIPITGNTINSIDVLNGVASGFMVIASFSLSLCLFIIQNYEIAIISLPLGFVSLAFYKYHKIPSKIFPGDSGALTLGAMYGAIAIVGGVEIIAAVALLPAVINSFLFLSSVKRIVEHRQIKGKPVEHTDDFKLKATDDKTAPVTLVRLILAGGPLTEKQVGFAIFRLAIFSGILAIITAFLMGVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR09</accession>
    <name>A0A2S2KR09_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Blue (type 1) copper domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_08450</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR09"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000923">
        <property type="entry name" value="BlueCu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052721">
        <property type="entry name" value="ET_Amicyanin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507:SF1">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00127">
        <property type="entry name" value="Copper-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Blue (type 1) copper" evidence="4">
        <location>
            <begin position="276"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="357" mass="37766" checksum="5DBEE63307F2CA12" modified="2018-07-18" version="1">MMPGMGLTAIGLAGLTLSYAGIAHTFIDGMHALTGLTMFVGLIFLSAGILDGGISTSNRAKATTLVILSIALGFGMFAFTMNTSNYTVTIAGILMAIAFPAIIIAYLAMKHPSFVKPVGAIVGMAAATGIIAWVAFGFVSPDTYMIPQEVGVEEPSEEILPTGPIFSISILEGSSQQGSPDYDPDVATVTQGYIIEWTNADSVAHTVTSAVDFGETFDSSLMDAGDVFTLDTNNLEVGEYEYMCIVHPWMVASLVIEAAKEPTKVTIPEGAAIPEDGKNYYDPKVIDVTVGTTVVWDNADNTMHTATSGSPDTGADGVFDSDILSAGDTFEFTFTDAGNYDYYCILHPWMIGTVNVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR16</accession>
    <name>A0A2S2KR16_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Translation initiation factor 6</fullName>
            <shortName evidence="3">aIF-6</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">eif6</name>
        <name evidence="4" type="ORF">NZNM25_06860</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Binds to the 50S ribosomal subunit and prevents its association with the 30S ribosomal subunit to form the 70S initiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eIF-6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876524.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR16"/>
    <dbReference type="GeneID" id="76210047"/>
    <dbReference type="OrthoDB" id="33582at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.75.10.10">
        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00032">
        <property type="entry name" value="eIF_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002769">
        <property type="entry name" value="eIF6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00323">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003134">
        <property type="entry name" value="PRK04046.3-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10784">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01912">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006413">
        <property type="entry name" value="IF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00654">
        <property type="entry name" value="eIF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2 3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="221" mass="23967" checksum="F981F8EB5DE3E2DA" modified="2018-07-18" version="1">MDIIKYDVYRGPNIGVYTKVNDSVILLPMGYAQSKAEKLAKYLGVEYRFMSIANTRLIGALCVMNNKGILIPKTAYQDEFDFLKNETDLEVGVLDSKLSALGNVICTNDKGAVVSPWLSPQDCKNISDVLGVEVIQKKIAGFNQTGAVMVANNSGAAIHPEADEEDMKTFSNLLGVKIEQCSINNGIPYVASGILVNNHSLVVGSLTTGPEIMMLTRAFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR36</accession>
    <name>A0A2S2KR36_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_09230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR36"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="125" mass="13858" checksum="B0A799077E78305B" modified="2018-07-18" version="1">MYKSQKIMGYKRRNNRYNKPNRRKGDARPKLAIIGAISLAIGILLVHYVGIPAIYSEGKFDDNPALIVGMVGIVSGVVMLLLSLNKKRQGQLAGGMEAVGKAFEPDCNCCKCTNCDRNHNHWTHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR60</accession>
    <name>A0A2S2KR60_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Nitrate/sulfonate/bicarbonate ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_09340</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876768.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR60"/>
    <dbReference type="GeneID" id="76208736"/>
    <dbReference type="OrthoDB" id="10909at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03293">
        <property type="entry name" value="ABC_NrtD_SsuB_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050166">
        <property type="entry name" value="ABC_transporter_ATP-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42788:SF13">
        <property type="entry name" value="ALIPHATIC SULFONATES IMPORT ATP-BINDING PROTEIN SSUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42788">
        <property type="entry name" value="TAURINE IMPORT ATP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="254" mass="28415" checksum="5722A674744BD691" modified="2018-07-18" version="1">MAKLEAKNIVKYFSHDSHKLKALGGVNLKVEAGDFVCLVGPSGCGKSTFLRIVAGLETPDDGQILFDGHPVAETGPERIMVFQEGALFPWLKVQDNVEFGLKMAGIPKEERAKISHRYLDMMQLTKFADSYTYQLSTGMKQRVAIARALVMDPDILLMDEPFAALDAQTRDLLLVEMQLIWEKTKKTILFVTHNVSEAAVLGTKVAIFSNRPSIIKKEVDNNFPHPRVTEDESLLKFQQDILAELRPEVKKSKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR62</accession>
    <name>A0A2S2KR62_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Methionine synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">NZNM25_09300</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>methylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:28115"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the vitamin-B12 dependent methionine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876764.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR62"/>
    <dbReference type="GeneID" id="76208740"/>
    <dbReference type="OrthoDB" id="134276at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008705">
        <property type="term" value="F:methionine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050667">
        <property type="term" value="P:homocysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046653">
        <property type="term" value="P:tetrahydrofolate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.20:FF:000007">
        <property type="entry name" value="Methionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.280:FF:000008">
        <property type="entry name" value="Methionine synthase (MetH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.280">
        <property type="entry name" value="Cobalamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="entry name" value="Dihydropteroate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1240.10">
        <property type="entry name" value="Methionine synthase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.196.10">
        <property type="entry name" value="Vitamin B12-dependent methionine synthase, activation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003759">
        <property type="entry name" value="Cbl-bd_cap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050554">
        <property type="entry name" value="Met_Synthase/Corrinoid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011822">
        <property type="entry name" value="MetH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036594">
        <property type="entry name" value="Meth_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004223">
        <property type="entry name" value="VitB12-dep_Met_synth_activ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037010">
        <property type="entry name" value="VitB12-dep_Met_synth_activ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45833">
        <property type="entry name" value="METHIONINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45833:SF1">
        <property type="entry name" value="METHIONINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02607">
        <property type="entry name" value="B12-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02965">
        <property type="entry name" value="Met_synt_B12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000381">
        <property type="entry name" value="MetH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01018">
        <property type="entry name" value="B12-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="Cobalamin (vitamin B12)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="Dihydropteroate synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56507">
        <property type="entry name" value="Methionine synthase activation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47644">
        <property type="entry name" value="Methionine synthase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50974">
        <property type="entry name" value="ADOMET_ACTIVATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51337">
        <property type="entry name" value="B12_BINDING_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 14" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="12" id="KW-0170">Cobalt</keyword>
    <keyword evidence="8 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Pterin-binding" evidence="17">
        <location>
            <begin position="21"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="B12-binding N-terminal" evidence="20">
        <location>
            <begin position="308"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="B12-binding" evidence="19">
        <location>
            <begin position="426"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="AdoMet activation" evidence="18">
        <location>
            <begin position="570"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="576"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="576"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="436"/>
            <end position="440"/>
        </location>
        <ligand>
            <name>methylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:28115"/>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>methylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:28115"/>
        </ligand>
        <ligandPart>
            <name>Co</name>
            <dbReference type="ChEBI" id="CHEBI:27638"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="484"/>
        </location>
        <ligand>
            <name>methylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:28115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="621"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="775"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="829"/>
            <end position="830"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000381-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000381-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="GBH34139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="23">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="832" mass="92639" checksum="CB477AB16420C5B4" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR96</accession>
    <name>A0A2S2KR96_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_08520</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876699.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR96"/>
    <dbReference type="GeneID" id="76208819"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF25">
        <property type="entry name" value="USPA DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="5"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="144" mass="16398" checksum="3F3DCB9584673508" modified="2018-07-18" version="1">MNALFEHILVPYNGNLDSEKAFRKAMSLAISIGAKITILTCLEERHTFGFKTKTSKQEFEKESKLVELQHQKLKKLAKEHDVSCDSKIVKNGLASIKILEFADKHNVGLIVMTKTKLASHYEKQHYHSTVENVFRNTSCPILIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRF2</accession>
    <name>A0A2S2KRF2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Isocitrate/isopropylmalate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_10510</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876864.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRF2"/>
    <dbReference type="GeneID" id="76208611"/>
    <dbReference type="OrthoDB" id="6813at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019298">
        <property type="term" value="P:coenzyme B biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000019">
        <property type="entry name" value="Homoisocitrate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF77">
        <property type="entry name" value="ISOCITRATE_ISOPROPYLMALATE DEHYDROGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="7">
        <location>
            <begin position="4"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="343" mass="37795" checksum="589C54AF666FB5A5" modified="2018-07-18" version="1">MSKKAAVMRGDGIGPEVVDSMLKVLKECNIQSEIILCEAGSEQWDKNGRKDKSYIPDETISILEESDACFKGPTTTIPVPGAPRSVAVTLRQKFELYSNIRPTKTFDRLTPDRKLDCVCFREATEGLYTGVEAKITDDAAIAIRKITRQGCDRFLNSAMNWAKQYNMKKMVAITKRNILKETDGIFWSSAQKAVEGTDVELSEIYIDNMAQQMVVAPEQFNGAVLVSTNLFMDIISELASGLVGSIGLIYSANMGDNFAMFEAAHGSAPQFAGQNKVNPTATVLSGAWMAEYLGEREIRDAIFDATYQVINEGKTVTWDIGGNASTTQMTDAIITYAKEKLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRJ6</accession>
    <name>A0A2S2KRJ6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Twin-arginine translocase TatA/TatE family subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_09780</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876807.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRJ6"/>
    <dbReference type="GeneID" id="76208691"/>
    <dbReference type="OrthoDB" id="10785at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.3310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003369">
        <property type="entry name" value="TatA/B/E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42982">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42982:SF1">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02416">
        <property type="entry name" value="TatA_B_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="12785" checksum="E44959BA1E3E808C" modified="2018-07-18" version="1">MLEHSLNVGGSEWLIIIFLALVLILGTGRLPGAARKLGKAVNEYNKAKNEIQDHMKEVTEESPKISGPVETEREKLEMIARTAGVKVEGKTDDELRKSISEKIGQKRIDESESKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRM1</accession>
    <name>A0A2S2KRM1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Adenylosuccinate synthetase</fullName>
            <shortName evidence="8">AMPSase</shortName>
            <shortName evidence="8">AdSS</shortName>
            <ecNumber evidence="8 9">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">purA</name>
        <name evidence="10" type="ORF">NZNM25_10970</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>IMP + L-aspartate + GTP = N(6)-(1,2-dicarboxyethyl)-AMP + GDP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8 9">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="8 9"/>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876946.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRM1"/>
    <dbReference type="GeneID" id="76208561"/>
    <dbReference type="OrthoDB" id="372247at2157"/>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046040">
        <property type="term" value="P:IMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03108">
        <property type="entry name" value="AdSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.440.10:FF:000005">
        <property type="entry name" value="Adenylosuccinate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.440.10:FF:000007">
        <property type="entry name" value="Adenylosuccinate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.170.10:FF:000002">
        <property type="entry name" value="Adenylosuccinate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.440.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.170.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00011">
        <property type="entry name" value="Adenylosucc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018220">
        <property type="entry name" value="Adenylosuccin_syn_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042109">
        <property type="entry name" value="Adenylosuccinate_synth_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042111">
        <property type="entry name" value="Adenylosuccinate_synth_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001114">
        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003295">
        <property type="entry name" value="PRK04293.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846:SF0">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01266">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="12"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <begin position="13"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <begin position="40"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="42"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="248"/>
            <end position="254"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="280"/>
            <end position="282"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="320"/>
            <end position="322"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="333" mass="35894" checksum="23D800E02C0DC227" modified="2018-07-18" version="1">MTSTVVVGGFFGDEGKGKIISYLAIKDNPTIIVRGGAGPNAGHTIRDGDKVYKVRMLPSGFLNKNAKVMIGPGVVINPEVLKKEINDFEVSGRSFIDKHCGIIEETHLIRDSKGELKDKIGSTGSGTGPANADRAMRVLKLAKDFDSLSSIITDVPQEINTALSKNEHVLVEGTQGTFLSLWHGTYPFVTSKDVTASGICADVGLGPTKVDEVIVVFKSYVTRVGTGPLEKELSLDEAEKKGWSEFGTVTGRQRRAADFDFDLARRAIMLNGATQISITKLDVLFSDCAGKTSFDELSDDAKSFIKNIENELNTPVTIIGTGPAVNDVIDRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRQ8</accession>
    <name>A0A2S2KRQ8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thioredoxin family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876992.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRQ8"/>
    <dbReference type="GeneID" id="76208504"/>
    <dbReference type="OrthoDB" id="334137at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02969">
        <property type="entry name" value="PRX_like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047262">
        <property type="entry name" value="PRX-like1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43640">
        <property type="entry name" value="OS07G0260300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43640:SF1">
        <property type="entry name" value="THIOREDOXIN-DEPENDENT PEROXIREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="10"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="184" mass="20809" checksum="F895572C3BA586A3" modified="2018-07-18" version="1">MVLLESQVKLKTGSIAPDFELLGIDDKKHSLADYSNYKGILVIFMCNHCPYVKAKADALNELYEKFGKEIAIIGINSNDSTDYPEDSFEAMKETAKEKGFKFDYLVDKTQEIAKKYGAMCTPDPFLFNSQKQLVFHGRIDNAMKPDDTATEKTMINNIQKLLAGEKIENDFDPSIGCSIKWKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRS9</accession>
    <name>A0A2S2KRS9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosomal RNA large subunit methyltransferase E</fullName>
            <ecNumber evidence="5">2.1.1.166</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">23S rRNA Um2552 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">rRNA (uridine-2'-O-)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rlmE</name>
        <name evidence="8" type="ORF">NZNM25_10680</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>uridine(2552) in 23S rRNA + S-adenosyl-L-methionine = 2'-O-methyluridine(2552) in 23S rRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42720"/>
            <dbReference type="Rhea" id="RHEA-COMP:10202"/>
            <dbReference type="Rhea" id="RHEA-COMP:10203"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74478"/>
            <dbReference type="EC" id="2.1.1.166"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.166" evidence="5"/>
    <dbReference type="EMBL" id="BGKI01000005">
        <property type="protein sequence ID" value="GBH34277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876924.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRS9"/>
    <dbReference type="GeneID" id="76208592"/>
    <dbReference type="OrthoDB" id="26307at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF13">
        <property type="entry name" value="PRE-RRNA 2'-O-RIBOSE RNA METHYLTRANSFERASE FTSJ3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005461">
        <property type="entry name" value="23S_rRNA_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="7">
        <location>
            <begin position="20"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5 6">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR005461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="197" mass="22259" checksum="4A241A2A1BA5F45F" modified="2018-07-18" version="1">MKLIDARKDHFRRLAHEQGFRSRAAFKLQELNKSYRIIGPGFYVLDLGCAPGGWTQMAVKLAGNQGKVMGVDLSYVEEIPGAHIIRENIEDEHVVDEVMTYFGRKVNAVICDLSPQVSGNWSVDHAKQISLNYDCTKIMDKVLANKGNAVFKVFDGEYSMEFRDYVKKKFARINLTKPSASRKQSSELYLVCLGFIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRT3</accession>
    <name>A0A2S2KRT3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_10420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146195995.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRT3"/>
    <dbReference type="GeneID" id="76208622"/>
    <dbReference type="OrthoDB" id="12300at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="206" mass="23410" checksum="442BA768EFC44882" modified="2018-07-18" version="1">MKARLLILAMVVFWTTFDSSFAESQEFTVAEVKWVKSSHSSDGFASIQVIEPDMNLSPNDIEKFKIHIWSDSDPNGITPDVYETSKDSGVFASNIYFSPNSSTGQRIHVLEEDLVIASYEDQTLPISYTDEKLEVFDSMIIRKTLANPDENQGFFRIDDPTFVRQNLQTGETLGDASSIFGWFVLAMSLFIVFAYITMKIKMRIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRU2</accession>
    <name>A0A2S2KRU2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877035.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRU2"/>
    <dbReference type="GeneID" id="76209323"/>
    <dbReference type="OrthoDB" id="295712at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18127">
        <property type="entry name" value="GAPDH_II_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02278">
        <property type="entry name" value="GAPDH_II_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003251">
        <property type="entry name" value="PRK04207.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="349" mass="39451" checksum="7A1699D89CDFEE6E" modified="2018-07-18" version="1">MKKVFVNGYGSIGSRITSFLKDDPEIIVVGVGKYSPDEKVEDAISKGLDVYVPASKIPSFSNYKIAGSIESVLDSCDLVIDAAPGGSGYKNKINLYEPKNIPAIYQGGETVVGNEAVSDLLFNSRVNYDQALGKRHVMQGSCNVTGMGRILEPLRVKFGDRLIRFDVTLVRRWADIEQTEKKVVDTIEMSEKPHHGDDVKMYFGKEAPLYVRAIKVPTRQMHLHIMDIRFKDVAPKPSEIHELFTNEFGVATIWTAKGTKDIRDYAEKMGFNFTDTNMIHIHANMTTSIDDTVQMMYSDDQTGIVIPENHMLLQAMLFEKSYEDAFAHTESIFHMKEKKEKLQEYFAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRV5</accession>
    <name>A0A2S2KRV5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ArnR1-like winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_09690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876799.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRV5"/>
    <dbReference type="GeneID" id="76208701"/>
    <dbReference type="OrthoDB" id="1242at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="110" mass="12779" checksum="D3BD305148A8E7BA" modified="2018-07-18" version="1">MAESKKYRDRIYIVKDIILTLSEYGELNQTSLFSFCGLNITKHKEILNNLEENDIIQRIETVEGKRIITIFKVTPKGMNFCHEILEPYEQLFPRSGKSDSSVNLGCLFFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRW1</accession>
    <name>A0A2S2KRW1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_10980</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876947.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRW1"/>
    <dbReference type="GeneID" id="76208560"/>
    <dbReference type="OrthoDB" id="10902at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="153" mass="17602" checksum="1EF40EF80730A7B2" modified="2018-07-18" version="1">MVGENFAGNIIINLANLPDFLRNPILKKRMMEFFSLPELDQKEVINNALEAGPTIPFPNFSKLFRTWLKILTTLSEEQRKGLFTAYIVEVAQSPQKLISFNLDGILEVFLTLEENEKEILADTIKKIINDLDENAKRRVMIVIPDNAKKHLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRX3</accession>
    <name>A0A2S2KRX3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleotidyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRX3"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="223" mass="25525" checksum="9A7C9E250D61B472" modified="2018-07-18" version="1">MLKAIILAGGRGKRLRPITDYIPKPLVPIKNIPIIEWQIKYLKKFGIDEIIICTGYKQDIIEHYLNIKNIGIKIKFSVEKSPLGTGGAIKKAAKLINEKSFFVINGDTITNIDLKELAKKQNSLASIELRTNFGILETNNDKITKFREKKEIVGVWMNAGIYHLDKEILRDLPDKGDIEKTVFPNYAKKGKLSTVKFKNVRWYSIDSFKDMEECALEIEKIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRZ1</accession>
    <name>A0A2S2KRZ1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_10160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876837.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRZ1"/>
    <dbReference type="GeneID" id="76208651"/>
    <dbReference type="OrthoDB" id="3051at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="451" mass="51798" checksum="9CDAE9D9CBD1970F" modified="2018-07-18" version="1">MTENNNSIEFRVIHKRRNRNENNDDPHQCKTIVKIEPHYMQRFGIKEGEIVKISGNTKNTVAMCLPMSDSDLQEIDTREIEVEFLNNPEKKVQHPPKIILYGPVSCNVDILGGWKQTINLSKFPESQIKSESIPEAELVTLATVNIAEKTMPGYHSHLDYSEIMGFVVTNDDRIDIPFQKEWHEKKQQEMWEKENQHPSSDIGTIRGNRSSSHLPSFPKSFQSAIVDVTPEGKPFWKITENTRFEFKDANLAKIFDPYRFTPKKLVNVVSISKQILVDDTEFTIPSLEVYSNMMKLIWYSYQRIKIPESDFTDIRKMNHMNRMMRNEHPRLIITLEDDLGNRYASTNESGGGGGSSGPDPTTMEIISNYSWHSIFSSTLDPKAKEIILTIKEVQWVRQNTTSMNMPPPPPRPSPHMTPIENTLLPKIVIAEGPWTFNVPVHWQKSSSDEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS07</accession>
    <name>A0A2S2KS07_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 9">Peptide chain release factor subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Translation termination factor aRF1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">prf1</name>
        <name evidence="11" type="ORF">NZNM25_12430</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 9">Heterodimer of two subunits, one of which binds GTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the eukaryotic release factor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877086.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS07"/>
    <dbReference type="GeneID" id="76209261"/>
    <dbReference type="OrthoDB" id="1011at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000032">
        <property type="entry name" value="Eukaryotic peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000003">
        <property type="entry name" value="Peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.960.10:FF:000003">
        <property type="entry name" value="Peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.960.10">
        <property type="entry name" value="eRF1 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00424">
        <property type="entry name" value="Rel_fact_arch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024049">
        <property type="entry name" value="eRF1_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020918">
        <property type="entry name" value="Peptide_chain-rel_aRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004403">
        <property type="entry name" value="Peptide_chain-rel_eRF1/aRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03676">
        <property type="entry name" value="aRF1_eRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10113">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03463">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55481">
        <property type="entry name" value="N-terminal domain of eukaryotic peptide chain release factor subunit 1, ERF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="10">
        <location>
            <begin position="7"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="425" mass="47519" checksum="74EEC5A1A8850BBD" modified="2018-07-18" version="1">MKKIDVEKQDSVKLYKIRKILEELSKKSGRGTELITVYIPKGKQLHEIISSLQQEQGTADNIKSDLTRSHVVDSLGKVVQRLKMYKKTPEKGLVVFCGALPPEGGGPLGSEIVTVWEIEPPKDLNQYLYRCDDHFHVDILKDMLKDDNLIGFLAIDAKDAGWGLLHGDKIEVLAQTGSGVAGKHRQGGQSAKRFQKLREMELTYYFNRVAETTKEYFIDIYPIKGLVISGPGPTKEDFINGNYLEYRLQNMIINTIDASYSGAEGIREAFGKSSDILGDFRMVEEKKLIEDLFREINTNSGKGSYGLKEVIEFLKNNVVKVVLITDNTNLHRVEGICKRCKHFQEEIVERPQVIPKKTEFANSPCPGCNAMEVEVNEQDIVDYLELLAAKTGSQLEVISGSSEHGNMLASLGKIGAILRYNPGHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS71</accession>
    <name>A0A2S2KS71_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">N-formylglutamate deformylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hutG</name>
        <name evidence="1" type="ORF">NZNM25_12800</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866887.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS71"/>
    <dbReference type="GeneID" id="76209220"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.40">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007709">
        <property type="entry name" value="N-FG_amidohydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05013">
        <property type="entry name" value="FGase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="255" mass="29239" checksum="6E0BC7A9D9AFCE6D" modified="2018-07-18" version="1">MLSIPHGGRKKPIELDGHLCITNKDLFDDSDPFVIEIFDLGDKVQRVIKTNIARAFIDLNRSQDDLPPKNPDGLIKSSTCYAKPIYLDRKEPDPSLRTLLIELYYLPYHNSIQKNLDELKLQLCLDCHSMASFAPSISPDGKNNKRPKFCLSNNDGQTASEEMLELLASCISKSYEIDRNEISLNDPFHGGYITKTYGNNPVPWIQIEMNRDLYLSDPWFDQETLTVDLTRLQKLNEQFENSLNLFFKEFDGVDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS84</accession>
    <name>A0A2S2KS84_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">threonine ammonia-lyase</fullName>
            <ecNumber evidence="3">4.3.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_12020</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the serine/threonine dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.19" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877051.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS84"/>
    <dbReference type="GeneID" id="76209304"/>
    <dbReference type="OrthoDB" id="9915at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04886">
        <property type="entry name" value="ACT_ThrD-II-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01562">
        <property type="entry name" value="Thr-dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000007">
        <property type="entry name" value="L-threonine dehydratase catabolic TdcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044561">
        <property type="entry name" value="ACT_ThrD-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005789">
        <property type="entry name" value="Thr_deHydtase_catblc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01127">
        <property type="entry name" value="ilvA_1Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF6">
        <property type="entry name" value="L-THREONINE DEHYDRATASE CATABOLIC TDCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="6">
        <location>
            <begin position="18"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="402" mass="43108" checksum="DF7F98544E259EE9" modified="2018-07-18" version="1">MNPSYDEILKANSLRGNEIKKTPLIHSSTFSEFTNSDVYLKLEFRQKTGSFKIRGAYYKIQSLSEEEKKYGVVAASAGNHAQGVAFASALEKIPCTIVMPKNASPAKVAATKGYGAKVILEGTNYDESSAKAKEIAKITKATMIHAFDDPQIIAAQGVIGLEILEDLPDVDEVYVPIGGGGLAAGTAIAIKEKNPKIKVIGVQSRSFPSMYDSYKKGSITASGGARTIADGISVKVPGKLTFEIIKELIDDIVLVDDTEITKAMFLLMERMKFVVEPAGAISLAHLISKKPSPGKKVVAILAGGNVDMYLLGQIVDKGLAAMGRLLKLSILLPDRPGAFKEIVDEITLANANIVEVVHDRLSSNINAGSAGVTLSLETQGQEQANLLIEALRRKNIQFTLMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS95</accession>
    <name>A0A2S2KS95_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_12120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877060.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS95"/>
    <dbReference type="GeneID" id="76209292"/>
    <dbReference type="OrthoDB" id="1920at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="103" mass="10791" checksum="B332134B9761E15B" modified="2018-07-18" version="1">MSEKKTIMAAGVMLSLLILPSVSLAFAQYIPSQGQTGLDDLMKLSEERVRIAKENPHTGSGTPMFALDGVLGALLLSTGVFGGIATAFFVKGRKGKYAAMGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSA2</accession>
    <name>A0A2S2KSA2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Roadblock/LAMTOR2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12850</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSA2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046600">
        <property type="entry name" value="DUF6659"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20364">
        <property type="entry name" value="DUF6659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="15012" checksum="6759C19E6CB37E2A" modified="2018-07-18" version="1">MIIMALLTAKEIKKLDDACLELSKDEKVRHVGIINEHGRLIAGGFKKGKSPLIKDEKISMTYMQMQLDFKMRKELDDVLGPIDYIASRRTKQLIISVPIGDNLILISAEPDADDKQIIKKAEELFDDITISTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSB3</accession>
    <name>A0A2S2KSB3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Apea-like HEPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12480</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877090.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSB3"/>
    <dbReference type="GeneID" id="76209256"/>
    <dbReference type="OrthoDB" id="1913at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="184" mass="21372" checksum="1E017A2979E69EA9" modified="2018-07-18" version="1">MSVPHFIASIKGKKRISSKIRLYLIDKEKHYFLNDGVLKNGFNPKLSISKNRDSVLSAFSKMAFLFDEIIRLRIIGYSNNSDSADLLYLLNLVPVNRKIRTFLDWKVFAPEFTRNMSRLFEVRNATVHCISLSEVNYAPKNKLSLSSTSGFNKFVKDFQKAWGVLLKIYVKEQEKLDWKKLSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSD2</accession>
    <name>A0A2S2KSD2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877091.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSD2"/>
    <dbReference type="GeneID" id="76209252"/>
    <dbReference type="OrthoDB" id="1398at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="60" mass="6719" checksum="95E15ABB027092C3" modified="2018-07-18" version="1">MVDYDLGEYETIMIESKIREGVFHKVVKDLKSGKAVSCSCKCWSKGNKPCVGMRTVGFDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSE7</accession>
    <name>A0A2S2KSE7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_160049222.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSE7"/>
    <dbReference type="GeneID" id="76209136"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="53" mass="5954" checksum="28E43F97265391AE" modified="2018-07-18" version="1">MGKYCENCGKYMKISELTHCSDECLLSGIKDSTSLSENGFNAISWDEKSDPWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSH4</accession>
    <name>A0A2S2KSH4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_14030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877215.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSH4"/>
    <dbReference type="GeneID" id="76209096"/>
    <dbReference type="OrthoDB" id="6319at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="15682" checksum="E54BD5F11C189437" modified="2018-07-18" version="1">MNGNQNLYDPSEMFKSWIQKSGRAQAEFIKNFGALMTNQPGQTFNPLETLKGVSESTRKAQSDIMENVASMQNKSMDTMFNIGQMLPSFMNWGAYKTTLSSNGRISIPEAERNALGLSDGDLVQVIILPIAKKSKNKEVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSJ4</accession>
    <name>A0A2S2KSJ4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Deoxyhypusine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_13120</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the deoxyhypusine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSJ4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034038">
        <property type="term" value="F:deoxyhypusine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.910.10">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002773">
        <property type="entry name" value="Deoxyhypusine_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036982">
        <property type="entry name" value="Deoxyhypusine_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001980">
        <property type="entry name" value="PRK00770.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703:SF2">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01916">
        <property type="entry name" value="DS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="359" mass="40431" checksum="7A0A9E4470F065D7" modified="2018-07-18" version="1">MFVKVDPHKFHGKDIPHIKLDPNMTIEDLVDVFASSGYNGRQLGDAAKLYARMIEEDATICLTVSGAMTPVGFGGIIKTLIERGFVDWIITTGANVYHEDHFAWGLPVKQGSFDVDDMKLYENEIVRIRDVYIKFHETLEAEDELIQKMFGDDFPDKPFTTAEFCNLMGKISKEKAKHPEKSFITTAYDYDVPVYISTIKDSSLALNLAVHRLRDKIYNLDFVREIIEQAAILYDSKKSGILELGGGVPKNTAQQTGPLLDQILRRNDGGQDYVIQITDARPDTGGLSGATLQEGKSWGKVQDAHHGMVTVYADVTIAFPILALYVLSNQKTREPKRLYKKLNKLYEKLKDDYSKNPTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSK6</accession>
    <name>A0A2S2KSK6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Elongation factor 1-alpha</fullName>
            <shortName evidence="11">EF-1-alpha</shortName>
            <ecNumber evidence="11">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Elongation factor Tu</fullName>
            <shortName evidence="11">EF-Tu</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">tuf</name>
        <name evidence="13" type="ORF">NZNM25_14430</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">GTP hydrolase that promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="11"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877246.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSK6"/>
    <dbReference type="GeneID" id="76209052"/>
    <dbReference type="OrthoDB" id="371718at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03693">
        <property type="entry name" value="EF1_alpha_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000005">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000020">
        <property type="entry name" value="Translation elongation factor EF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000204">
        <property type="entry name" value="Translation elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_A">
        <property type="entry name" value="EF_Tu_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008969">
        <property type="entry name" value="PRK12317.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11 13" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="9 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="12">
        <location>
            <begin position="4"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="13"/>
            <end position="20"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="91"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="153"/>
            <end position="156"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH34652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="432" mass="47096" checksum="733AE8A29FA15A6B" modified="2018-07-18" version="1">MADKPHLNLIVTGHIDNGKSTTMGHFLMDLGVVDERTIAAHGAESEKTGKGDTFKYAWVMDNIKDERERGITIDLAFQKFESPKYFFTLIDAPGHRDFIKNMITGASEADAAILVLSAKEGETDTAIAAGGQAREHAFLLKTLGVSQLIVAINKMDAVEYKEDAFNAAKEKGEKLVRSVGYKLENVPFIPVSGWKGDNLVKRSENMAWYKGKTLLEAFDDFTIAEKPVGKPLRVPIQDVYTITGVGTVPVGRVETGIMKAGQKIIVMPSGAQGEIKSIETHHTEMPSAEAGDNIGFNLRGIEKKDIKRGDVLGTPDAPPMVAKEFKAQIIVIHHPTAIAPGYTPVMHAHTAQVAATVTEFLQKINPASGAVEEENPKFLKVGDSAIVKIRPVRPTCIETFQEFPEMGRFALRDMGATIAAGIVKEITEEYKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSP8</accession>
    <name>A0A2S2KSP8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_15010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877296.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSP8"/>
    <dbReference type="GeneID" id="76208995"/>
    <dbReference type="OrthoDB" id="5797at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="68" mass="8069" checksum="E72B816B145CFA2D" modified="2018-07-18" version="1">MLESHTFFSKMVDELVEFSEHDPELADGIKWLDDQAQKKGITFYDMVFEVLYKHDVNSKAKEWLSTRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KST1</accession>
    <name>A0A2S2KST1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">Citrate synthase</fullName>
            <ecNumber evidence="6">2.3.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_15000</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>oxaloacetate + acetyl-CoA + H2O = citrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16845"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; tricarboxylic acid cycle.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 8">Belongs to the citrate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.16" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877295.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KST1"/>
    <dbReference type="GeneID" id="76208996"/>
    <dbReference type="OrthoDB" id="21302at2157"/>
    <dbReference type="UniPathway" id="UPA00223"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036440">
        <property type="term" value="F:citrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06110">
        <property type="entry name" value="BSuCS-II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="entry name" value="Citrate Synthase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="entry name" value="Cytochrome P450-Terp, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011278">
        <property type="entry name" value="2-MeCitrate/Citrate_synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024176">
        <property type="entry name" value="Citrate_synthase_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01800">
        <property type="entry name" value="cit_synth_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739:SF4">
        <property type="entry name" value="CITRATE SYNTHASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001369">
        <property type="entry name" value="Citrate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="active site" evidence="7">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001369-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="387" mass="43862" checksum="7E6867C27615BBC0" modified="2018-07-18" version="1">METKNIGLRGIEVADTKISNIDGERGRLIYRGYDILELTKNSTFEETAYLLLYDDLPTRNQLEEFNSKLIDARFIPKQMQKNMGNWRKDADPMDMLQAFVAALAGYYDEEFANKEASYDKAINLIAKVPTIIASWNRIRNGLDIVDPDPTLSHAANFLYMMSGERPDSEVEKIFDVCLILHADHTFNASTFTARQVASTRAHMYSACSAAIGALSGELHGGANTEVMKMLLEIGDIEKVEKWIKEKMSKGERIMGMGHAVYKTYDPRAQVLKQLSRKLAEKTKEPWFAITEKIETVTISEMKSQKNKDIYPNVDLYSASLYYMLKIPMDLNTPIFAISRVVGWAAHIIEEKFAEAAPKPALYRPKATYVGKYCGPEGCEYKTLDLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSU4</accession>
    <name>A0A2S2KSU4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877132.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSU4"/>
    <dbReference type="GeneID" id="76209193"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="59" mass="6830" checksum="8F0CC0C9C4716565" modified="2018-07-18" version="1">MGDDRFTLKSLACEKEPIHPVDVKKVHSILNITYTKGDNSMVQRKKITEEQGRCYDHPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSW4</accession>
    <name>A0A2S2KSW4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Sugar kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_15710</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877400.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSW4"/>
    <dbReference type="GeneID" id="76208416"/>
    <dbReference type="OrthoDB" id="206224at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009384">
        <property type="term" value="F:N-acylmannosamine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008761">
        <property type="term" value="F:UDP-N-acetylglucosamine 2-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24066">
        <property type="entry name" value="ASKHA_NBD_ROK_EcFRK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049874">
        <property type="entry name" value="ROK_cs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964:SF149">
        <property type="entry name" value="BIFUNCTIONAL UDP-N-ACETYLGLUCOSAMINE 2-EPIMERASE_N-ACETYLMANNOSAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="ROK (REPRESSOR, ORF, KINASE) FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01125">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="288" mass="31125" checksum="037D741C57F4BB69" modified="2018-07-18" version="1">MYKIGIDLGGTKTEVILLDENLRALERKRIPTPQNDYEEIINNISSLVLEVSENISDFSIGVCTPGAISKQTGLIKNSNTQCLIGNSLKQDLEKKLGKKISIENDANCFTMAEAKMGAAMDYGLVFGVIMGTGIGGGIVIDGNLHSGRTNIAGEWGHHILHRNGNSCYCGKAGCVETYISGPSLEKQWKKQTGKSMSLPEILSNIDNKVGQKWKDEFLENFGYGLANVIDILDPDAIVLGGGLSNIDFLYTEGKKLVYDKVFSDLVDTPILKNKLGDSAGVYGAALLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSX7</accession>
    <name>A0A2S2KSX7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2203 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877157.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSX7"/>
    <dbReference type="GeneID" id="76209162"/>
    <dbReference type="OrthoDB" id="7857at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018699">
        <property type="entry name" value="DUF2203"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09969">
        <property type="entry name" value="DUF2203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016498">
        <property type="entry name" value="UCP016498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="15466" checksum="48C5C523BBFBC40A" modified="2018-07-18" version="1">MFSFFTTNEANVALPDVIKKFEYALSKKNEVSKLEQQLQMCISTTNSFEEFITLKQKLNSAVTKFYEAVEILENTGVSVKSIEQGLLDFPSKRFDEEVWLCWKYGETEIKFWHEKDSGFMGRKPIEVNDESLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSY4</accession>
    <name>A0A2S2KSY4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_15400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877375.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSY4"/>
    <dbReference type="GeneID" id="76208448"/>
    <dbReference type="OrthoDB" id="10016at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="322" mass="35196" checksum="44DA48C236DB2EF6" modified="2018-07-18" version="1">MKFQILFSILIVLLIVNTPNSFSELELFTNSKVYAPSHTLQVYGKGLPEENLIIRIFAPDESIAKFDQITTKSDGSFNYGLMTWPQPSTNLPFGTYTVEVISTQQNGISQKIDVKFSATTDLVDVPVERIVNTLVFAPETAAINNPIRVFVQTTSDGLLIGNEPAELLGTTHVHLPSGISIPLSNSFKTLHQGLYYVDYIPREEGTHVFHVVAFTQGTTSHGSAATNVLSQDLGGISEQIIKLNSILDETSEELDVLKSEIAGFDTTLKRASIQIDENIGTISTSVKYITEASSQLNALMLPIIASIGLIVALQVAILTRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT14</accession>
    <name>A0A2S2KT14_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein eS27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rps27e</name>
        <name evidence="6" type="ORF">NZNM25_15750</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eS27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877404.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT14"/>
    <dbReference type="GeneID" id="76208412"/>
    <dbReference type="OrthoDB" id="5718at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.100">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00371">
        <property type="entry name" value="Ribosomal_eS27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000592">
        <property type="entry name" value="Ribosomal_eS27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023407">
        <property type="entry name" value="Ribosomal_eS27_Zn-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001629">
        <property type="entry name" value="PRK00415.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01667">
        <property type="entry name" value="Ribosomal_S27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="zinc finger region" description="C4-type" evidence="5">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="65" mass="6837" checksum="2193EF1EF687DE42" modified="2018-07-18" version="1">MKKDHIEIPKPASKFQKVNCNECGELQVVYSHASTQVACNSCGNVIAEATGSKAKINGKVSGSAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT16</accession>
    <name>A0A2S2KT16_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_15120</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877306.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT16"/>
    <dbReference type="GeneID" id="76208984"/>
    <dbReference type="OrthoDB" id="3010at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="127" mass="14493" checksum="73BC9BD3FCFC1F9A" modified="2018-07-18" version="1">MKEQVYGAEKGNGEKVASWLNEQAKDSDLKLQVKLKNSVLSTQNFGDFDLLLLSDDSIKSRKLISKAGKRFGIKMIEGGYKEEARIIRRKKSDYAKVLNGDKVIGHLELETSRFGTKKWEIKSEERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT39</accession>
    <name>A0A2S2KT39_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CARDB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_14160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146196001.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT39"/>
    <dbReference type="GeneID" id="76209079"/>
    <dbReference type="OrthoDB" id="11801at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35902:SF3">
        <property type="entry name" value="NPCBM-ASSOCIATED, NEW3 DOMAIN OF ALPHA-GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35902">
        <property type="entry name" value="S-LAYER DOMAIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="580"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="624" mass="66607" checksum="3E805A81209143C3" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT79</accession>
    <name>A0A2S2KT79_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_16300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT79"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07581">
        <property type="entry name" value="nitrilase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001110">
        <property type="entry name" value="UPF0012_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088:SF27">
        <property type="entry name" value="DEAMINATED GLUTATHIONE AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088">
        <property type="entry name" value="NITRILASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01227">
        <property type="entry name" value="UPF0012"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="1">
        <location>
            <begin position="22"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="289" mass="32553" checksum="A36AFE18119EA608" modified="2018-07-18" version="1">MVHFMFVPRVYPFIAIQLIYFVKAAVIQFKASTKKEDNLKKIVSYISKAAAKNATLCAFPEFMMFFTNSSQTSKQLASLAETINDTFVTTIAKAAKDNHIQVVGSFYEKSKKKDRVYDTSFVIDKSGKVISTYRKIHLYDALGFKESDKMISGSKIAKPVKTSIGKIGMMICYDLRFPEMSRSLAVAGSEVLIIPSAWVKGNMKEEHWITINKTRAIENGCYIIAPDQVGNIYCGRSLVVDPYGKILLDMKKKQGIGFVNIDLDIVKQTRKVLPLLKNRRTDVYPTLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTB6</accession>
    <name>A0A2S2KTB6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PEFG-CTERM sorting domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_14960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866904.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTB6"/>
    <dbReference type="GeneID" id="76208999"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027560">
        <property type="entry name" value="PEFG-CTERM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04296">
        <property type="entry name" value="PEFG-CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="260" mass="28491" checksum="B157B74A77FDAB15" modified="2018-07-18" version="1">MVSTLAFANTTIEVQTSETKIKALESIWVTGKITDVAQYKPVKLRVIGPDGKLVFAPQVTIDDNGEFRKLLNPPIPSFKPGEYIVTASHDDTKSVAQTQFTVISQEIPRNSDIQTSESKVIVEKEQQKSSKEITIQAEAENGSDVIMVKGNTNFRGTDITLIVKSPIGNMITIDQVTPGMYGDFQAEIKIGGPMWKEDGMYTITANHGSSSEYKESIKVEIKDGVVVPEFGVTTSLVLAMSIISIIIVSTKLKLATPFRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTD4</accession>
    <name>A0A2S2KTD4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amidohydrolase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_16080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877445.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTD4"/>
    <dbReference type="GeneID" id="76208378"/>
    <dbReference type="OrthoDB" id="42910at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050287">
        <property type="entry name" value="MTA/SAH_deaminase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005552">
        <property type="entry name" value="PRK07213.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794">
        <property type="entry name" value="AMINOHYDROLASE SSNA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794:SF5">
        <property type="entry name" value="CHLOROHYDROLASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="1">
        <location>
            <begin position="52"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="396" mass="43689" checksum="37827257501E4D48" modified="2018-07-18" version="1">MLIRNISVLQGSELDFVLRTNVKIQNNVFQKIQSKIQTNSNEESVDCEGLLLIPGFINAHTHIGDSIGKDVTLNSSVDEKIHPVFGAKSKILKNTSQENLSNFMKNTCHSMLRKGITTFVDFREGGLDGVILLKKVLSNIPIRSIILGRLEFYQKSTEIKKNLPLPKEKTEDLNQLLKECDGIGVSGANENSTSILEYYSKTPKLRAIHSSETIQSISASKKVTGKSETIRALSLKPHFLVHMTFASKSDLTISAKKTRGIVICPRANSSLAEGIPDIELMQKAGCTLALGTDNVMINSPDMFREMDFLWKATMGIHKKRIDPKEILKMSTVNGGKVLNKDIGVIENGKIADCIFLDKHALDLEPMHNPHASIVHRASESAIKAVMIGGKIVYGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTH0</accession>
    <name>A0A2S2KTH0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_17340</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877545.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTH0"/>
    <dbReference type="GeneID" id="76208251"/>
    <dbReference type="OrthoDB" id="9014at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004061">
        <property type="term" value="F:arylformamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.50:FF:000001">
        <property type="entry name" value="Kynurenine formamidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.50">
        <property type="entry name" value="Putative cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007325">
        <property type="entry name" value="KFase/CYL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037175">
        <property type="entry name" value="KFase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31118">
        <property type="entry name" value="CYCLASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31118:SF12">
        <property type="entry name" value="CYCLASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04199">
        <property type="entry name" value="Cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102198">
        <property type="entry name" value="Putative cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0823">Tryptophan catabolism</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="213" mass="23919" checksum="AED1C0D93720ED08" modified="2018-07-18" version="1">MKPIDLTLTISKSIPSFPGSPKPQFILWSDIKDDGYNLELLFLSSHTGTHIDAPYHFVKNGIKIHQIPLDRLIGKAILIKLKKLKNSQITKQDIMIFEKNNGIIPNHSSVFFFTQWQKNLQKENYFTENPGLNKSAANYLISKKINLVGIDSPSIDLGNDSSFPVHHILSKNNILIVENLTNLNKISSIEFNFTILPLKLKDATGSPVRALAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTH5</accession>
    <name>A0A2S2KTH5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Imidazoleglycerol-phosphate dehydratase</fullName>
            <shortName evidence="6">IGPD</shortName>
            <ecNumber evidence="6">4.2.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">hisB</name>
        <name evidence="7" type="ORF">NZNM25_15490</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:11040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="EC" id="4.2.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the imidazoleglycerol-phosphate dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.19" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877382.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTH5"/>
    <dbReference type="GeneID" id="76208439"/>
    <dbReference type="OrthoDB" id="103579at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00011"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004424">
        <property type="term" value="F:imidazoleglycerol-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07914">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.40:FF:000001">
        <property type="entry name" value="Imidazoleglycerol-phosphate dehydratase HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.40:FF:000003">
        <property type="entry name" value="Imidazoleglycerol-phosphate dehydratase HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.40">
        <property type="entry name" value="Imidazole glycerol phosphate dehydratase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00076">
        <property type="entry name" value="HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038494">
        <property type="entry name" value="IGPD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000807">
        <property type="entry name" value="ImidazoleglycerolP_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002114">
        <property type="entry name" value="PRK00951.2-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133:SF2">
        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133">
        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE HIS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00475">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="195" mass="21649" checksum="78FD11485239A5A6" modified="2018-07-18" version="1">MVIRKASIKRNTKETSVQVSANIDGTGKTSIKTGINFLDHLMVSFGKHGMMDLKVNAKSNDGIEHHLIEDTAITLGQAIDKALGSRKGITRFSYASVPMDESLAEASIDLIKRPFWKLTLLIKRNKIEDISKEDLEHFFQSLLQNLNSCIHLTVKYGDNDHHKVEAAIKSLAVAFRNASLMDKKQKGIPSTKGSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTL9</accession>
    <name>A0A2S2KTL9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ACT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_17850</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTL9"/>
    <dbReference type="OrthoDB" id="56543at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="25042" checksum="3FE7EF8B92A6E7BB" modified="2018-07-18" version="1">MRVASMSIPEIVREIITRNRSIYDCMKMDLINYTALAVKIQPEIEKILGNSVNLNTVVVAIKRYADSFESKDEVKDGSVLKNARLALTDGIMDIKFSVKESGEMDPMAILDKFSKITNNYEFFRLSDSFRFLTEDLEDIRQIFNNVPSREDMFSTGLAKIRISIPNAQNQSDVVSYVAEVLHANGIELVNAFFSQDGIIIILNEKDASRAYDILHSDIMRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTN1</accession>
    <name>A0A2S2KTN1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_17930</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTN1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="78" mass="8860" checksum="61534862047E2BEB" modified="2018-07-18" version="1">MQMVFSYQVVKFQTISFVQGTHWSQSVGEKGILYKSLKDPFSKIIIQSSNGSKKLYHVPKDRTVVVTNDIVHFLGELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTQ6</accession>
    <name>A0A2S2KTQ6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD-dependent protein deacylase</fullName>
            <ecNumber evidence="3">2.3.1.286</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Regulatory protein SIR2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cobB</name>
        <name evidence="6" type="ORF">NZNM25_17520</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">NAD-dependent lysine deacetylase and desuccinylase that specifically removes acetyl and succinyl groups on target proteins. Modulates the activities of several proteins which are inactive in their acylated form. Deacetylates the N-terminal lysine residue of Alba, the major archaeal chromatin protein and that, in turn, increases Alba's DNA binding affinity, thereby repressing transcription.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>N(6)-acetyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-acetyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:43636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="ChEBI" id="CHEBI:83767"/>
            <dbReference type="EC" id="2.3.1.286"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>N(6)-succinyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-succinyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:11877"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:87830"/>
            <dbReference type="ChEBI" id="CHEBI:87832"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="3">2 residues (Tyr-64 and Arg-67) present in a large hydrophobic pocket are probably involved in substrate specificity. They are important for desuccinylation activity, but dispensable for deacetylation activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sirtuin family. Class III subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.286" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877560.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTQ6"/>
    <dbReference type="GeneID" id="76208233"/>
    <dbReference type="OrthoDB" id="728at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017136">
        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036054">
        <property type="term" value="F:protein-malonyllysine demalonylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036055">
        <property type="term" value="F:protein-succinyllysine desuccinylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01412">
        <property type="entry name" value="SIRT5_Af1_CobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01121">
        <property type="entry name" value="Sirtuin_ClassIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027546">
        <property type="entry name" value="Sirtuin_class_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001753">
        <property type="entry name" value="PRK00481.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF10">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 3" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3 4">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="97"/>
            <end position="100"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 4">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 4">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 4">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 4">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="182"/>
            <end position="184"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="208"/>
            <end position="210"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="240" mass="26921" checksum="D8527FCECF38EBC9" modified="2018-07-18" version="1">MFELIKDQIKNIKKVVFVTGAGISQESGIPTFRGKDGLWKNHDSMKLATIDAFYDNPKLVWEWYNERRKNIFAAQPNLGHKAIAELEKYVKVVVLTQNIDGLHQKAGSSEVLELHGSIIKIKCSVCNFNEEITSEISEILPLCKCGNILRPDVVWFGESLPQDVWQKAMIYASQCDLMVIVGTSLVVSPANTLPIYAKQNNALLIEINPDNTEISPEMTLVIRNTGTISLPEFVSLFKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTR0</accession>
    <name>A0A2S2KTR0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">4Fe-4S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_17380</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007402606.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTR0"/>
    <dbReference type="GeneID" id="56064223"/>
    <dbReference type="OrthoDB" id="5583at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050572">
        <property type="entry name" value="Fe-S_Ferredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687">
        <property type="entry name" value="ADENYLYLSULFATE REDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687:SF6">
        <property type="entry name" value="L-ASPARTATE SEMIALDEHYDE SULFURTRANSFERASE IRON-SULFUR SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="5"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="44"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="99" mass="10984" checksum="DF9AEB6F3547F6C6" modified="2018-07-18" version="1">MPVGIIPDISEQMCIGCALCVEICTTLGPDVLRVKPVEGWKRGKAFVFYPERCISDGACIGVCPTKAIFWMRPMDFTVGQPVPLYKNSVFVKGWTELID</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTR8</accession>
    <name>A0A2S2KTR8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">High-affinity zinc uptake system binding-protein ZnuA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">znuA</name>
        <name evidence="4" type="ORF">NZNM25_16490</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTR8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006129">
        <property type="entry name" value="AdhesinB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050492">
        <property type="entry name" value="Bact_metal-bind_prot9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006128">
        <property type="entry name" value="Lipoprotein_PsaA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006127">
        <property type="entry name" value="ZnuA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42953:SF3">
        <property type="entry name" value="HIGH-AFFINITY ZINC UPTAKE SYSTEM PROTEIN ZNUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42953">
        <property type="entry name" value="HIGH-AFFINITY ZINC UPTAKE SYSTEM PROTEIN ZNUA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01297">
        <property type="entry name" value="ZnuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00691">
        <property type="entry name" value="ADHESINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00690">
        <property type="entry name" value="ADHESNFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53807">
        <property type="entry name" value="Helical backbone' metal receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="293" mass="32447" checksum="BABE1D69498F2664" modified="2018-07-18" version="1">MAIIAIAIIIPLSSLVVYGTDSKPQLSNTNNSKLQVISSFNPLYEFSQNVGQEKVDVTLLIPVGVEPHDWEPTVKDVQRMQNADLIIINGIGFENWVDNLEDTGYSGEIIDTSNGILIKTDEISSVSGDPHIWLNPVYAKIQVQNIANAFSNSDPANEQYYQSNAAKYIEELDLLDSKIRNELSDCNNNFISFHDAFSYFADEYNLNQHTIISSNDPHGEATAKTLENIISTAKNLNINIIFAEESVDTRTSTIIANEIGGKVLVLSPLEVVTDGTYISRMTENLENLKEALC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTT9</accession>
    <name>A0A2S2KTT9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_17680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTT9"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="69" mass="7440" checksum="076741E83BA33E12" modified="2018-07-18" version="1">MAGFFHPLITNTSFDSVIIGVLILFVGLAGGILLYKAATSEKKREIFLGAGFTLMTISLYYIIAIAGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTV8</accession>
    <name>A0A2S2KTV8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">3-methyl-2-oxobutanoate hydroxymethyltransferase</fullName>
            <ecNumber evidence="5">2.1.2.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Ketopantoate hydroxymethyltransferase</fullName>
            <shortName evidence="5">KPHMT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 9" type="primary">panB</name>
        <name evidence="9" type="ORF">NZNM25_16890</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = 2-dehydropantoate + (6S)-5,6,7,8-tetrahydrofolate</text>
            <dbReference type="Rhea" id="RHEA:11824"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5 8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodecamer; pentamer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the PanB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.11" evidence="5"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877654.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTV8"/>
    <dbReference type="GeneID" id="76208297"/>
    <dbReference type="OrthoDB" id="8414at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003864">
        <property type="term" value="F:3-methyl-2-oxobutanoate hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06557">
        <property type="entry name" value="KPHMT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000003">
        <property type="entry name" value="3-methyl-2-oxobutanoate hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00156">
        <property type="entry name" value="PanB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003700">
        <property type="entry name" value="Pantoate_hydroxy_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00222">
        <property type="entry name" value="panB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001452">
        <property type="entry name" value="PRK00311.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20881">
        <property type="entry name" value="3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20881:SF0">
        <property type="entry name" value="3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02548">
        <property type="entry name" value="Pantoate_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000388">
        <property type="entry name" value="Pantoate_hydroxy_MeTrfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 9" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="5 6">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <begin position="44"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>3-methyl-2-oxobutanoate</name>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>3-methyl-2-oxobutanoate</name>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>3-methyl-2-oxobutanoate</name>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000388-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000388-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000388-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="279" mass="30561" checksum="D85F9B75F23FCCEA" modified="2018-07-18" version="1">MHKSVLDILKMKKEKKKISVITSYDYTLASLCDKAGIDVLLVGDSAGMVMLGYENTIPVTMDQMCMFTEAVSRARKNALLISDLPFMSYQSSIEDAINNSGKLIKAGADAVKLEGGSIMTETINAIVDIGIPVMGHIGLQPQTTMLSEGYKVQGKTKESAMKLIEDAKKLERAGVFSIALEMVSHEVAEIISQTVSVPIIGIGSGVGCDGQVLVVQDLLGMYDKIKPKFAKRYMNLSEDIVKSLEDYKKDVNSEAFPAEENWFSMDDDELKKLREQIGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU07</accession>
    <name>A0A2S2KU07_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative methylthioribose-1-phosphate isomerase</fullName>
            <shortName evidence="2">M1Pi</shortName>
            <shortName evidence="2">MTR-1-P isomerase</shortName>
            <ecNumber evidence="2">5.3.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">MTNA-like protein</fullName>
            <shortName evidence="2">aMTNA</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">S-methyl-5-thioribose-1-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_19250</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>5-(methylsulfanyl)-alpha-D-ribose 1-phosphate = 5-(methylsulfanyl)-D-ribulose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19989"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="ChEBI" id="CHEBI:58548"/>
            <dbReference type="EC" id="5.3.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EIF-2B alpha/beta/delta subunits family. MtnA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.23" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877738.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU07"/>
    <dbReference type="GeneID" id="76210155"/>
    <dbReference type="OrthoDB" id="45195at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046523">
        <property type="term" value="F:S-methyl-5-thioribose-1-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.420:FF:000003">
        <property type="entry name" value="Methylthioribose-1-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10470:FF:000006">
        <property type="entry name" value="Methylthioribose-1-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.420">
        <property type="entry name" value="translation initiation factor eif-2b, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01678">
        <property type="entry name" value="Salvage_MtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005251">
        <property type="entry name" value="IF-M1Pi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011559">
        <property type="entry name" value="Initiation_fac_2B_a/b/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027363">
        <property type="entry name" value="M1Pi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00524">
        <property type="entry name" value="eIF-2B_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004326">
        <property type="entry name" value="PRK05720.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00512">
        <property type="entry name" value="salvage_mtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475:SF1">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1 2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="53"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="252"/>
            <end position="253"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="2">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="351" mass="37977" checksum="B0383A28D2A28D1E" modified="2018-07-18" version="1">MDNTAVDSSLRTVEWKNNKVIMIDQTKLPNQLVFVEYDDFNQVADAIKTLIVRGAPAIGVSGAFGLALAVLQSKATTKDELISDLEIARKILYSTRPTAVNLGWGLDKIMKIAKTGETVEQIKKLVIETAKKMAEDDIEINKAMGKNGSILFDDNDTIMTHCNAGALATVAYGTALGVIRATRESGKNIKVIATETRPIQQGSRLTAFELKHDGFDVSLIPDTAVGYSMANGLVNKVVVGADRIVKTGHVFNKIGTYQVATMAKQHGIPFYVAAPLSTIDLETKAEDVIIEMRKGNEVTGIGDKKTAPDDINVINPAFDMTPPELISGIITEKGVARAPYEESIPKLFQAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU35</accession>
    <name>A0A2S2KU35_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Capsule polysaccharide biosynthesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_19710</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877780.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU35"/>
    <dbReference type="GeneID" id="76210201"/>
    <dbReference type="OrthoDB" id="3332at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015774">
        <property type="term" value="P:polysaccharide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007833">
        <property type="entry name" value="Capsule_polysaccharide_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05159">
        <property type="entry name" value="Capsule_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="519" mass="61537" checksum="9EBA6846E2049967" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU63</accession>
    <name>A0A2S2KU63_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">YjbQ family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_19840</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877791.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU63"/>
    <dbReference type="GeneID" id="76210214"/>
    <dbReference type="OrthoDB" id="6663at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.460">
        <property type="entry name" value="YjbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001602">
        <property type="entry name" value="UPF0047_YjbQ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035917">
        <property type="entry name" value="YjbQ-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00149">
        <property type="entry name" value="TIGR00149_YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YJBQ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615:SF2">
        <property type="entry name" value="YJBQ FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01894">
        <property type="entry name" value="YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004681">
        <property type="entry name" value="UCP004681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111038">
        <property type="entry name" value="YjbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="15882" checksum="D99C9464AE00D971" modified="2018-07-18" version="1">MKSLTEYLTFEVKTRRAFVNITNDVRDLVVKSKVKEGLCLVNAMHITASVFINDNEGGLLHDYEKWLEGLAPHEPTNHYDHNKTGEDNADAHLKRQVMGREVVIAITNGKLDFGPWEEIFYGEFDGKRPKRVLVKIIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU66</accession>
    <name>A0A2S2KU66_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UDP-N-acetyl-D-mannosamine dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_20010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877805.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU66"/>
    <dbReference type="GeneID" id="76210234"/>
    <dbReference type="OrthoDB" id="7951at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="379" mass="42049" checksum="C6966CE69BD4BFC7" modified="2018-07-18" version="1">MGGAKVVVYGLSTEGYAIASQMAIKGADVYIIDESTPSAISLKAEIAKTYPNVASLKEDEPLLAMEPIDVAISKAQYLFFTPRIRKTGQDIKTEIHSKFKDAVTSLKKNSSVVFTLPTGFGGNNENISLLEHVTGLEAGKHISYFYYPLEDLNHQPKIIGSFNGKEDPVLHDLLTVGKKEKKFVAISSSEHFHAINVLSRFSSLCSVLEVCKYAQDDVTKHDLSSDDFQEIFLDNMVSGLFDLKALGSSFEGANSLMYLINGSVKGIDGYIKRLIDEIRATLKKKDLKASRTKIALSWTLDQHAMRGDKIEMLQNLTSRLRDYIGDVEAYEDPNFDLFHSDKTTIIVACSKNDFDIIEKTKQGSELIIVKANPLCETIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU81</accession>
    <name>A0A2S2KU81_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transport permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_20040</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877809.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU81"/>
    <dbReference type="OrthoDB" id="147058at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047817">
        <property type="entry name" value="ABC2_TM_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051784">
        <property type="entry name" value="Nod_factor_ABC_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43229">
        <property type="entry name" value="NODULATION PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43229:SF2">
        <property type="entry name" value="NODULATION PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006648">
        <property type="entry name" value="DrrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="55"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="134"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-2" evidence="6">
        <location>
            <begin position="19"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH35213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="253" mass="27797" checksum="83C430E38769BF1B" modified="2018-07-18" version="1">MHPIVRLVNRNLTISLNPGFLIWQVIFPLIYIFVAGFAYAPLINQVPFGAKDLDYPAFLASGMIGFNIMNSTLVSGIIIWNDRRHGMFEQIMSGPFTRSHYILSNICTIGIVGLVSATLIALVGYPVFFESVEFSLITIPIIIFGAITGSVLFGSLASIISTRLRSSEGFNVIINTVFLFFAFVSTAFYPAAGAPEPLKSAFYLNPLTYLVDVIRAGIFGNITEFVIFEMIILVGIASILFVIASKLLTKLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUA2</accession>
    <name>A0A2S2KUA2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zinc-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_20250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877842.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUA2"/>
    <dbReference type="GeneID" id="76210259"/>
    <dbReference type="OrthoDB" id="8738at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="57" mass="6529" checksum="F7C1608523CDAB81" modified="2018-07-18" version="1">MDARCPECEKVAILDDDITNVKCPHCNFEADYDSYLEIMKDQAINMATDYIPDRPGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUA3</accession>
    <name>A0A2S2KUA3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Probable translation initiation factor IF-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">infB</name>
        <name evidence="11" type="ORF">NZNM25_18460</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8 9">Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877671.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUA3"/>
    <dbReference type="OrthoDB" id="30957at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03703">
        <property type="entry name" value="aeIF5B_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000112">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10050:FF:000001">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00100_A">
        <property type="entry name" value="IF_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029459">
        <property type="entry name" value="EFTU-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004544">
        <property type="entry name" value="TF_aIF-2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00491">
        <property type="entry name" value="aIF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003078">
        <property type="entry name" value="PRK04004.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF4">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14578">
        <property type="entry name" value="GTP_EFTU_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 8 9" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="10">
        <location>
            <begin position="3"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="77"/>
            <end position="81"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH35055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="593" mass="65817" checksum="14E7DFE146071365" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUB8</accession>
    <name>A0A2S2KUB8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carbohydrate-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_18390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUB8"/>
    <dbReference type="OrthoDB" id="11811at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49344">
        <property type="entry name" value="CBD9-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="266" mass="30871" checksum="330E15F8B52FAE94" modified="2018-07-18" version="1">MKNKLKILNILFIILFFPIFFESVHAEDPILISISSDLDQVIFDGKWTHAYEWKQSSYNRFVFDDGNEIHLRTGHQDNFIYVHINFASDTNIDKGSDNAMICFDTKNDKTIIPQSDDYCFSTTLDTKNSFTYRGNNSSIDDSFDIISNDKNFVAKGTASDKYDRYSKVPHTSYEFRIPLDVLGRSDNYGFYVSVYDGDSQNHYSWPYEIKNQTSFSSPSQWGDLVSPDKSLPEFDLLIMFSLTFSLMTVSIIITKFNMFKINTFRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUV5</accession>
    <name>A0A2S2KUV5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Ribosome biogenesis protein Nop10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nop10</name>
        <name evidence="8" type="ORF">NZNM25_20190</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Involved in ribosome biogenesis; more specifically in 18S rRNA pseudouridylation and in cleavage of pre-rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NOP10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877831.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUV5"/>
    <dbReference type="GeneID" id="76210253"/>
    <dbReference type="OrthoDB" id="7259at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.40">
        <property type="entry name" value="H/ACA ribonucleoprotein complex, subunit Nop10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00803">
        <property type="entry name" value="Nop10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007264">
        <property type="entry name" value="H/ACA_rnp_Nop10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036756">
        <property type="entry name" value="H/ACA_rnp_Nop10_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023532">
        <property type="entry name" value="Nop10_arc-typ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009623">
        <property type="entry name" value="PRK13130.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13305:SF0">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13305">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN NOP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04135">
        <property type="entry name" value="Nop10p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144210">
        <property type="entry name" value="Nop10-like SnoRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 7" id="KW-0698">rRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH35228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="51" mass="6189" checksum="E4E1E30594B94153" modified="2018-07-18" version="1">MKFQLRKCIKCNQYTLKEKCPRCGEKTISAHPAKFSPDDKYMRYRLAERYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUW4</accession>
    <name>A0A2S2KUW4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA primase small subunit PriS</fullName>
            <ecNumber evidence="11">2.7.7.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">priS</name>
        <name evidence="14" type="ORF">NZNM25_20290</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalytic subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. The small subunit contains the primase catalytic core and has DNA synthesis activity on its own. Binding to the large subunit stabilizes and modulates the activity, increasing the rate of DNA synthesis while decreasing the length of the DNA fragments, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.</text>
    </comment>
    <comment type="function">
        <text evidence="13">RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterodimer of a small subunit (PriS) and a large subunit (PriL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11 12">Belongs to the eukaryotic-type primase small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="11"/>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUW4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.920.10">
        <property type="entry name" value="DNA primase, PRIM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00700">
        <property type="entry name" value="DNA_primase_sml_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002755">
        <property type="entry name" value="DNA_primase_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023639">
        <property type="entry name" value="DNA_primase_ssu_PriS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10536">
        <property type="entry name" value="DNA PRIMASE SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01896">
        <property type="entry name" value="DNA_primase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56747">
        <property type="entry name" value="Prim-pol domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 11 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3 11 12" id="KW-0639">Primosome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 11 12" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="11">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GBH35238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="374" mass="43237" checksum="EA4C28FCBDBDF0C9" modified="2018-07-18" version="1">MMQDSDIKFLEDSFKKYYFEHFDLIRVQERTSEREFGYQKFNSGMTRHIQIKDDRELHLLFMQNIPSDVYCSNAYYSFPNLPMNEKDWKEADLIFDIDAKDLNLPCRENHTISICNECSQVSNDSGQCRKCNSTKLEKKSLPCKNCIDSSKIQVEKLSEILVDDFAIDKENIHVYFSGNEGFHVYVYNSQFQKIGSRERSELSDYIMLRGAIPETFGMKKIKSNRSSFPDFDDSGWKGRFSKYVFGTKSKRSKIISELLVNGYSSFQKTLDDVSENIGVKIDPNVTMDVHRIFRLPGSINSKSGLTKILCKDLAKFDPYTEASFLSDDTVEVFANSPIEFKLKNKKFGPYKNEKVTIPTFAAVYMICKKLARIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNL7</accession>
    <name>A0A2S2KNL7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3D domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_00010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNL7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019867">
        <property type="term" value="C:outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009254">
        <property type="term" value="P:peptidoglycan turnover"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010611">
        <property type="entry name" value="3D_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06725">
        <property type="entry name" value="3D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3D" evidence="1">
        <location>
            <begin position="183"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="251" mass="28576" checksum="69D874B805D69E2A" modified="2018-07-18" version="1">MINMTSVVVKNYLIILLFVLGIVHLSGYAAFAEDLYFPTWIFTVYDFLIHEKISYEEFGNVIQYLQEHRIVQLVMNYDYDPVTNFLITSSIENKITQSQFLNCSSDWYVTGYFTPVEDDYAGELIGILLNDEIQYFKSDFISVIKTEGWGKTNSGMYLGWYDESFHMSDSPLDSHGNILLVPSIAVDSELIKQKTNLIIPKLPYPWNEIVFESSDIGTSIKGKHIDVYTGEGKAAELETFRITGAGNDICS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNM3</accession>
    <name>A0A2S2KNM3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">8-amino-7-oxononanoate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_00110</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875895.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNM3"/>
    <dbReference type="GeneID" id="76209337"/>
    <dbReference type="OrthoDB" id="9071at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF77">
        <property type="entry name" value="8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="5">
        <location>
            <begin position="42"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="377" mass="42758" checksum="41AA9F9A07001046" modified="2018-07-18" version="1">MASNHKLNFVDSELENLKRLNLYRKLRYGKSKGPHIIINNKKLLNLCSNDYLGIHSSQIQINQLQSSSRLVSGNDESYKKLESILAKHKSQQNSLVYPTGYMANLGAISAIAKKKDIILSDELNHASIIESCKLTDSKIIIYKHNDMQDLEKKLKLKVNNKFVITEGIFSMDGDFSSLKQISELSEKQNAITIVDDAHGDFVVGKDGSGTPSYFNVEKKIDLYISSLSKGLGSFGGYVASKNNVIDLCINKSKSFIYTSALPSFLIEYSIKRFKSNREERKKKLERNTRKLIKGLKQIGFEINSTTHIIPIIIGDEKKAIEFGEFLMNRGVFAQPIRYPTVPKNKARLRISVTAWLSDKDIERSLEIFEKAFRKFCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNP1</accession>
    <name>A0A2S2KNP1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF371 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNP1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.630">
        <property type="entry name" value="mth639 domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007171">
        <property type="entry name" value="DUF371"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023131">
        <property type="entry name" value="Mth639-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40696:SF1">
        <property type="entry name" value="DUF371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40696">
        <property type="entry name" value="DUF371 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04027">
        <property type="entry name" value="DUF371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="146" mass="16396" checksum="66D5FFADA05E030D" modified="2018-07-18" version="1">MSFFYSSIVKFEIEFFGHENIRSNHQKTIEITKESHLTPQGDCIVGVNATSSCADLPLELKNKLKNSNSKIKFTISVGDDEFILEGRGHPELILAHTKDIVIRKSDFICPRTLAIKCDKASDLLPRTMVSLLQNPKTKGTFTIDID</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNR4</accession>
    <name>A0A2S2KNR4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_00330</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875915.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNR4"/>
    <dbReference type="GeneID" id="76209359"/>
    <dbReference type="OrthoDB" id="372044at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1440.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_uL5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_uL5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057266">
        <property type="entry name" value="Ribosomal_uL5_euk/arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_uL5_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003258">
        <property type="entry name" value="PRK04219.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL5 N-terminal" evidence="6">
        <location>
            <begin position="8"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL5 C-terminal" evidence="7">
        <location>
            <begin position="64"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="173" mass="18809" checksum="63D6B2ECE5FFC8D0" modified="2018-07-18" version="1">MSQTTEPIMKKISLEKIVLNMGVGKSGDSINIAKKALDQISGKKSSSRAAKETQRDWGVRKGEPIGVAVTIRGDDAKELLKRLLEAKGNTVNGKSFDNFGNYSFGIKEHIDIPGVKYDPQIGILGLGISITLTRPGYGIRNRSKHKASVGKKHIISNQEAKDYLVKEFGVTVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNS4</accession>
    <name>A0A2S2KNS4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="12">5-formaminoimidazole-4-carboxamide-1-(Beta)-D-ribofuranosyl 5'-monophosphate synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_00600</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875938.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNS4"/>
    <dbReference type="GeneID" id="76209389"/>
    <dbReference type="OrthoDB" id="84677at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006188">
        <property type="term" value="P:IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023656">
        <property type="entry name" value="IMP_biosynth_PurP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009720">
        <property type="entry name" value="IMP_biosynth_PurP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010672">
        <property type="entry name" value="IMP_biosynth_PurP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38147:SF1">
        <property type="entry name" value="5-FORMAMINOIMIDAZOLE-4-CARBOXAMIDE-1-(BETA)-D-RIBOFURANOSYL 5'-MONOPHOSPHATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38147">
        <property type="entry name" value="5-FORMAMINOIMIDAZOLE-4-CARBOXAMIDE-1-(BETA)-D-RIBOFURANOSYL 5'-MONOPHOSPHATE SYNTHETASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06849">
        <property type="entry name" value="DUF1246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06973">
        <property type="entry name" value="DUF1297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004602">
        <property type="entry name" value="ATPgrasp_PurP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMP biosynthesis enzyme PurP N-terminal" evidence="10">
        <location>
            <begin position="19"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="IMP biosynthesis enzyme PurP C-terminal" evidence="11">
        <location>
            <begin position="177"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH33269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="363" mass="41636" checksum="256CEC6BF8403DE3" modified="2018-07-18" version="1">MIKSSEIKKIVNEYSDIKIGVLGSHSALEVMDGAKDENFQTTVFCQKGREEPYQRFRRIADEIIILDKFKDMASAKYQKMLRDSNTIIVPHRSLTVYLGYKTIENSFKVPIFGNRKLFQAEERTAKKGQYYLLEKARIKYPKLFKDPKKINKPCIVKVQEKNRPLERAFFTVSSYKDFVEKSEAKIKQGVISRKDLAKSSIEELAIGTYMNFNFFHTPISNQVDFIGIERRLQTNIHDYNALPAKQQLDINVDLQNIEVGHTPASIRESLLEKVIKMGDKFVAAVKREYAPGIIGPFSLQSVITKDLELIVYDVSLRVPGNPIVATTTPYTKYQYGQTFGVGRRIAMEIKRAQEEGRLDEIVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNT9</accession>
    <name>A0A2S2KNT9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3303 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00150</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875899.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNT9"/>
    <dbReference type="GeneID" id="76209341"/>
    <dbReference type="OrthoDB" id="330at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="11446" checksum="936D942F4F2507EB" modified="2018-07-18" version="1">MNQETKPWVLVSSIQKETKPVDLEMITSQISTLVDEWQSHGKIMWSGAFDNQVSSMAVFEATEQEAKEFFKKYEKICSGILTYEMYQWDAMPILSILSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNU0</accession>
    <name>A0A2S2KNU0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_00800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875957.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNU0"/>
    <dbReference type="GeneID" id="76209409"/>
    <dbReference type="OrthoDB" id="11646at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="141" mass="16001" checksum="F812D7C67A20A50F" modified="2018-07-18" version="1">MSEKVWLGAIYLKDEGGYEIIIKSLKHYKNRLKTMGNSPELKNAAAMFASVLNQQAMKTIPVIDETIKKIQDNLSDIQSISKLSEDISFLEKALSCYEADINKAEDTGHEYFVNLVGNMIEARNDRKSIKVALRKIKQFSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNU2</accession>
    <name>A0A2S2KNU2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_00970</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875972.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNU2"/>
    <dbReference type="GeneID" id="76209425"/>
    <dbReference type="OrthoDB" id="115601at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF8">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="4"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="38"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="75" mass="8604" checksum="6F57869D9F4AEB96" modified="2018-07-18" version="1">MSQIQDLVEKGQSLMDDGKFDEALGFFEQALLLNQNDPDLWNYKGVALRSLGRYEESMECFNKSLEIDPRDKNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNV4</accession>
    <name>A0A2S2KNV4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator AsnC/Lrp ligand binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_01410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNV4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF6">
        <property type="entry name" value="REGULATORY PROTEIN ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="1">
        <location>
            <begin position="15"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="1">
        <location>
            <begin position="114"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="180" mass="19848" checksum="E09C9D1AC33F1CD9" modified="2018-07-18" version="1">MGCSIVARAYVLMNCNLGSEKEVISSLKKVAGVREAHGTLGLYDVIAQIELDTEEKIQQTVTQTIRRMPKIHSTMTLTRSESGELFQASEKLIGAMLGQNIIQAYIVIHCIKGEEYAVLKNLSHIPEVKEADVVFGLYDVICKVEASNSTILDHVITKAIRKIPDIISSITLHVVPEQDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNX0</accession>
    <name>A0A2S2KNX0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01610</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876030.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNX0"/>
    <dbReference type="GeneID" id="76209491"/>
    <dbReference type="OrthoDB" id="5993at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="97" mass="11273" checksum="396E12F6D9127D55" modified="2018-07-18" version="1">MIQREELEQILNFVANRWIETTKDPNSKALENLPNDFWMNSIGGKTAKKGFWVTTLMYTENEDDAASFKEVLLRWQAKGQDKNRDKGPDLIQIGKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP30</accession>
    <name>A0A2S2KP30_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_01970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876064.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP30"/>
    <dbReference type="GeneID" id="76209529"/>
    <dbReference type="OrthoDB" id="382035at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="212"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="238"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="350" mass="37359" checksum="D0BA3C1262CAD456" modified="2018-07-18" version="1">MTPFSFFLITLLTATVTVLPPGDTAIENNTADLESDETLDVSPNVKTFGTETKTIGGIQVELTKEVKFNSSDGGHLTIKNKSLTSATVTIPDQTKVKAPNSWNGKIIPPKQTSTSGTTPAGFQTPVTSISVGSVDVILVFDKAITILLEGVTGQTAFKTPGSDIWNLISTCVGTYENPVDPPINGECSISNGINTKIVTFHFTEFTELSTIPASSSTPTASTSSSGSSGGSSGGGGKRSSSGAPSSSQGYAGSLLPETTHSEFSVFPNWFQTSLVTYWIENLITDNEFKNAMNYMLNKNIIKIDVGEKKDIPTLDLAPSIKQLFKLWSLDKLTDSTIIEIIVYYRTVGAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP70</accession>
    <name>A0A2S2KP70_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Transcription initiation factor IIB</fullName>
            <shortName evidence="7">TFIIB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tfb_1</name>
        <name evidence="7" type="synonym">tfb</name>
        <name evidence="10" type="ORF">NZNM25_02610</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the TFIIB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876266.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP70"/>
    <dbReference type="GeneID" id="76209596"/>
    <dbReference type="OrthoDB" id="7429at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20549">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20550">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000023">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00383">
        <property type="entry name" value="TF2B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023484">
        <property type="entry name" value="TFIIB_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="9">
        <location>
            <begin position="7"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="repeat" description="1" evidence="7">
        <location>
            <begin position="123"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH33470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="302" mass="33155" checksum="52D6FA2DAE316F6E" modified="2018-07-18" version="1">MVKKLNPKDRCPRCGQGTVVTDANTGENFCGKCGFVITDKVEESGPEWRSFSNEGENKSRAGVPTSLAMHDMGLATVINPQNRDATGKPLTASMKSTIERLRTWDSRSQVHEPVDRNFRQAFSELDRLKDKLAVGDAVIEKAAYIYRKALEKGLVRGRSISALIASALYAACRDTETPRTLKDIGQASNIKRKDIARCYRLLLRELNLKMPVVDPVKCISRIASKAGLSEKTKRKATKILQTAEENKISAGKDPMGLAAAALYVACVTNGENKTQRDVAEAAGVTEVTIRNRYKGLKIALNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP93</accession>
    <name>A0A2S2KP93_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_02810</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP93"/>
    <dbReference type="OrthoDB" id="3983at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="71" mass="8240" checksum="50AF7474403A5D3D" modified="2018-07-18" version="1">MKPTKYMPKIETLDGAGFWKNAYAHQRGKLLKKVNVPEDQIIEMVNKKYMDLPAALRYEIETSGINKKDLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPB3</accession>
    <name>A0A2S2KPB3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876021.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPB3"/>
    <dbReference type="GeneID" id="76209482"/>
    <dbReference type="OrthoDB" id="383454at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="301" mass="34935" checksum="D7E98DA7E7B0D7DF" modified="2018-07-18" version="1">MGKISNSYNMTKSSTYDNELFKSELLYYVKSMKKSDFKTSNILANRIMTNAWFFDNLNYGIVGFQLRQFALDALNSSRVNNTNGAKQVTSEASSFSDFIITQLSSGKFNLTELWTKFNIQREKYRKSLLPPEEKDVYPSNPDFTNATFSKILEILNRRKGDVTFVTNNLLKGILNELNRTARTYGTKINETYLWCLIIMLERVDDYIAITAVDKVDFEKRTREEILPLIEEVLKIFEKGADPDSVSKLLWKMIATWRESFIRFLEPRPNPQPSLQFTPQEQAKEEIIDEFVEGIEGELVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPB4</accession>
    <name>A0A2S2KPB4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_00990</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875974.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPB4"/>
    <dbReference type="GeneID" id="76209427"/>
    <dbReference type="OrthoDB" id="11397at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051948">
        <property type="entry name" value="Hsp70_co-chaperone_J-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="118"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="183" mass="21715" checksum="1937E3A4816DD1FA" modified="2018-07-18" version="1">MNRISVFAILLIIFGTLQTAYPQSNNLDMELEVTDEEKIILFSGFAIAVIAIFMFLARDIILRKKTSYDKEEHESKKEKTYEKYHSDWGDDYEELGTRKNSKEDREFRDAVLNNELPNYYKILGLSKDATQEEIKKNFRELAKKNHPDKTKEDSEKQMMELNKAYEILSDKENKEKYDRYLTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPE1</accession>
    <name>A0A2S2KPE1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Dehypoxanthine futalosine cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_03440</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876192.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPE1"/>
    <dbReference type="GeneID" id="76209684"/>
    <dbReference type="OrthoDB" id="8186at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044689">
        <property type="term" value="F:7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045567">
        <property type="entry name" value="CofH/MnqC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022431">
        <property type="entry name" value="Cyclic_DHFL_synthase_mqnC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034405">
        <property type="entry name" value="F420"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020050">
        <property type="entry name" value="FO_synthase_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00423">
        <property type="entry name" value="CofH family radical SAM protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03699">
        <property type="entry name" value="menaquin_MqnC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076">
        <property type="entry name" value="FO SYNTHASE (COFH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076:SF1">
        <property type="entry name" value="LIPOYL SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19288">
        <property type="entry name" value="CofH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004762">
        <property type="entry name" value="CHP00423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00343">
        <property type="entry name" value="aminofutalosine_synthase_(mqnE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00342">
        <property type="entry name" value="cyclic_dehypoxanthine_futalosi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01064">
        <property type="entry name" value="F420__menaquinone_cofactor_bio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01389">
        <property type="entry name" value="menaquinone_synthsis_involved"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="8">
        <location>
            <begin position="59"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>(3R)-3-methyl-D-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:64642"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>(3R)-3-methyl-D-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:64642"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR004762-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR004762-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="371" mass="41413" checksum="B7E40235570D3EC6" modified="2018-07-18" version="1">MSQTTEQIQKSDIKDILENSLNGQRPGSKDCLRLLESDDVHLMGLVSGHLTQKKFGKKASFVNNIILNYTNVCITDCKFCAFYRSPGAADSYTLTLDQIESRVKTAWDMFKIRQVLIQGGHNPSLKIEYYEDAFRMIREKFPKVGVHGLSTSEIDMIAKVEKSSTKEILSRLKNAGLQSIPGAGAEILTDSVKNIISPKKISSDDWIRIMDEAHSLGIPSSATMMYGHVENKNDIVEHFFKIVKLQEKTKGFMAFIPWNFEPNNTLMHEEGLVEYGTGGIQLLKMIAISRLVFDGLISHIQSSWLTNGVGMAQLALQYGADDFGGTLIGEEVVSCTGARSTELTDKIIMDAIHQIGYDVEERDNFYNPVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPE9</accession>
    <name>A0A2S2KPE9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01920</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146195962.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPE9"/>
    <dbReference type="GeneID" id="76209523"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="116" mass="13219" checksum="1FC09071D79888C5" modified="2018-07-18" version="1">MYFIFFNPETREAIVDDVLSNELVQGKKPSISMPGINVPLMDYQNEKFEEVFVVCFLDENMMLPTFAFLNKDNAIAHMGKSDTTVWNASYSKNKAETSMPQSNQTIIHAKIKFAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPG1</accession>
    <name>A0A2S2KPG1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_02480</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutaredoxin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioredoxin family. DsbA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876110.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPG1"/>
    <dbReference type="GeneID" id="76209582"/>
    <dbReference type="OrthoDB" id="15256at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13887:SF14">
        <property type="entry name" value="DISULFIDE BOND FORMATION PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13887">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13462">
        <property type="entry name" value="Thioredoxin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Thioredoxin" evidence="7">
        <location>
            <begin position="14"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="219" mass="23907" checksum="A2E1DA8D8B89E7CE" modified="2018-07-18" version="1">MRKKAVILGLVAAIIVGITVSASSLPAETVNLDVGRMHGTISTAMGSAILGDPSAPITIVEFGDYQCHQCYNWFHNTKPAITRDYIETGKVNLVFVDLAFLGSDSPKAAQASYCAEDQGKYWDYHDLLYNSQEPKIDGGWANAERLKAFAFSLGLDMDLFDSCLDSGKYSKRVQYNIQQAKDHGVKGTPGFFIVGPDGQQQISGAQPFSVFKQILDPMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPM8</accession>
    <name>A0A2S2KPM8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable porphobilinogen deaminase</fullName>
            <shortName evidence="7">PBG</shortName>
            <ecNumber evidence="7">2.5.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Hydroxymethylbilane synthase</fullName>
            <shortName evidence="7">HMBS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Pre-uroporphyrinogen synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 10" type="primary">hemC</name>
        <name evidence="10" type="ORF">NZNM25_04070</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57845"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="EC" id="2.5.1.61"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>dipyrromethane</name>
            <dbReference type="ChEBI" id="CHEBI:60342"/>
        </cofactor>
        <text evidence="7">Binds 1 dipyrromethane group covalently.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="7">The porphobilinogen subunits are added to the dipyrromethane group.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the HMBS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.61" evidence="7"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876286.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPM8"/>
    <dbReference type="GeneID" id="76209753"/>
    <dbReference type="OrthoDB" id="8042at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004418">
        <property type="term" value="F:hydroxymethylbilane synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13644">
        <property type="entry name" value="PBP2_HemC_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000005">
        <property type="entry name" value="Porphobilinogen deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000086">
        <property type="entry name" value="Probable porphobilinogen deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.40">
        <property type="entry name" value="Porphobilinogen deaminase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00260">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000860">
        <property type="entry name" value="HemC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022417">
        <property type="entry name" value="Porphobilin_deaminase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022418">
        <property type="entry name" value="Porphobilinogen_deaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036803">
        <property type="entry name" value="Porphobilinogen_deaminase_C_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00212">
        <property type="entry name" value="hemC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557:SF0">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01379">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03900">
        <property type="entry name" value="Porphobil_deamC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001438">
        <property type="entry name" value="4pyrrol_synth_OHMeBilane_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00151">
        <property type="entry name" value="PORPHBDMNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54782">
        <property type="entry name" value="Porphobilinogen deaminase (hydroxymethylbilane synthase), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Porphobilinogen deaminase N-terminal" evidence="8">
        <location>
            <begin position="5"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Porphobilinogen deaminase C-terminal" evidence="9">
        <location>
            <begin position="221"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="modified residue" description="S-(dipyrrolylmethanemethyl)cysteine" evidence="7">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH33616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="311" mass="33873" checksum="0C11A325448AA933" modified="2018-07-18" version="1">MKYIVGARGSQLSLAQTNWVISELKKVNPGSEYEIKSITTKGDTDSRPLFTINQKGIFEKEVDRAVSQKEVDFAVHSLKDVPSELDESLVIACIPKREAVNDVFISSDGSSLENIKKGAVIGTSSLRRAVQVSRKRPDVTVKPIRGNIETRIKKTSGENFDAIVLAQAGISRLGVDVKFSPLPINDFSPSPGQGAIAIVARSDDTNTISMLKKIEDSDSRLEIEAERALSNYVDSGCRFPLGAYAKSSGTEMTLTVTAFSVDGKQSLHVTKTGSKNDPASLGKNVGEELRKKGVNDLALNWREKVEEWNTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPN2</accession>
    <name>A0A2S2KPN2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sodium-dependent bicarbonate transport family permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_04130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876292.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPN2"/>
    <dbReference type="GeneID" id="76209761"/>
    <dbReference type="OrthoDB" id="2566at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010293">
        <property type="entry name" value="Sbt_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40400">
        <property type="entry name" value="SLR1512 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40400:SF1">
        <property type="entry name" value="SLR1512 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05982">
        <property type="entry name" value="Sbt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="316"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="361" mass="38893" checksum="07B634F7032257BC" modified="2018-07-18" version="1">MDILQLIQSNLLTPIILFFLFGIIAARIKSDLKIPEAISEFLPIYLLAAIGLHGGIEMRNTGFENMLIPMLAAIGLSLLFTLNHYQILRRLGKFNIFDSYALASTYGAVGAVTFSVGLSFLKNQGVSSEGYLAAILAVLEPLAFILAIFLTNMAVSKQIKVKKKSFTADDASDIEIGIQSSKTKLSQVLHESITGKAIVILLGSIIIGYIIGENGFSSIKIVFDEMFTGAIVIFMIEMGIIAGQRLDDMKKVGIFLTAFAVIMPTFNGIVGVLVATVLGLSLGGAVMFGLLLASASFIAAPAVLRHTIPQAKPSLYITSALGITFPYNIIVLLPIMFAISSFVHTGEGSIDLFNYVIKDWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPP7</accession>
    <name>A0A2S2KPP7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Copper amine oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_04270</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876304.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPP7"/>
    <dbReference type="GeneID" id="76209775"/>
    <dbReference type="OrthoDB" id="28968at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019198">
        <property type="entry name" value="Beta_propeller_containing"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09826">
        <property type="entry name" value="Beta_propel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="738" mass="83918" checksum="F30CAA7B4D8149E5" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPR1</accession>
    <name>A0A2S2KPR1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_03750</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876220.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPR1"/>
    <dbReference type="GeneID" id="76209717"/>
    <dbReference type="OrthoDB" id="9652at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050595">
        <property type="entry name" value="Bact_response_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591:SF3">
        <property type="entry name" value="RESPONSE REGULATORY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591">
        <property type="entry name" value="STRESS RESPONSE REGULATOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="122"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="249" mass="28055" checksum="F28294034DF9A6D3" modified="2018-07-18" version="1">MNTKINCEIALTHLKNKQFITAHNLFLDQAESLKKSEYLKSALLFMLAAECKKRQGKESENEIREAGNLFVKYSKLENSPNMRGALLCASKCFLLQGEFDKAKDAYQKAKVMIPPKIHVTRPIVIVDDSKAISMKLQNYVEKLGYNEIHIFENGKDAIKGCKKLYSENKSPIVLLDMGLPDLEGDVVASKLLKENLHLQIIVITADEKNTSRVNKTISSGVSAFIQKPFTLDELKKSIDITESEYSLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPU1</accession>
    <name>A0A2S2KPU1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exosome complex component Rrp42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rrp42</name>
        <name evidence="7" type="ORF">NZNM25_04640</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Non-catalytic component of the exosome, which is a complex involved in RNA degradation. Contributes to the structuring of the Rrp41 active site.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the archaeal exosome complex. Forms a hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers. The hexameric ring associates with a trimer of Rrp4 and/or Csl4 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RNase PH family. Rrp42 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876334.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPU1"/>
    <dbReference type="GeneID" id="76209816"/>
    <dbReference type="OrthoDB" id="30932at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11365">
        <property type="entry name" value="RNase_PH_archRRP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00622">
        <property type="entry name" value="Exosome_Rrp42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050590">
        <property type="entry name" value="Exosome_comp_Rrp42_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020869">
        <property type="entry name" value="Rrp42_archaea"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003282">
        <property type="entry name" value="PRK04282.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097:SF8">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="5">
        <location>
            <begin position="33"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="6">
        <location>
            <begin position="191"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="272" mass="29308" checksum="46D0E90E2021C3F4" modified="2018-07-18" version="1">MTSTSVLDDLKKSQILELLEQGKRVDGRALDEPREIFIETGAIPKANGSARVRLGETEVICGVKIQPDRPFPDMGDKGIFICTAELLPLSHPTVETGPPGPDVIELARVVDRGIRESHMIDVSQLVIEKNKSVIGVFADNVVVDYDGNLFDACSYAAAAALLSSKTPKWNWIDDAPTLVEGEETSVPIATIPVSVTMGKIGNYIIVDPNGDEWASMDARITITSDSDGNICALQKGGSDGFTQDEINRCGEISVRVGAKIREKLKQAQMAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPU9</accession>
    <name>A0A2S2KPU9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Heavy metal resistance protein CzcA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_04600</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829035.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPU9"/>
    <dbReference type="GeneID" id="76209812"/>
    <dbReference type="OrthoDB" id="121419at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901255">
        <property type="term" value="P:nucleotide-excision repair involved in interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20075">
        <property type="entry name" value="XPF_nuclease_XPF_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150:SF0">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC4" evidence="8">
        <location>
            <begin position="7"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Helix-hairpin-helix DNA-binding motif class 1" evidence="7">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Helix-hairpin-helix DNA-binding motif class 1" evidence="7">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="25692" checksum="8AFAAD3D946D3DA8" modified="2018-07-18" version="1">MKLENLRIIVDERERKSGIPELLKSVGLNLEMKTLPIGDYIVGPETIVERKSIRDLMASVFDGRLFDQCSRLKEHFENPIVLMEGNVDEIEEITENPLIFYGAISTVVLDFKIPVIPTPSAVHTAKLLVSMCSRKESYKGPYLKKIKKSSDIERQQLSVLCSLPGIGDKFAVRMLEKFGTPLKVLTATTADLAKVEGLGEARAKKIKNMLDSKSKHLKKSDQKTLHDII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPX3</accession>
    <name>A0A2S2KPX3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Protein translocase subunit SecY</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Protein transport protein SEC61 subunit alpha homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">secY</name>
        <name evidence="15" type="ORF">NZNM25_05130</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the Sec protein translocase complex. Heterotrimer consisting of alpha (SecY), beta (SecG) and gamma (SecE) subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. May interact with SecDF, and other proteins may be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 13">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876375.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPX3"/>
    <dbReference type="GeneID" id="76209864"/>
    <dbReference type="OrthoDB" id="371914at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="entry name" value="SecY subunit domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01465">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026593">
        <property type="entry name" value="SecY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030659">
        <property type="entry name" value="SecY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019561">
        <property type="entry name" value="Translocon_Sec61/SecY_plug_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00967">
        <property type="entry name" value="3a0501s007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006341">
        <property type="entry name" value="PRK08568.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="SECY/SEC61-ALPHA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10559">
        <property type="entry name" value="Plug_translocon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="Preprotein translocase SecY subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00755">
        <property type="entry name" value="SECY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00756">
        <property type="entry name" value="SECY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="287"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="408"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="433"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Translocon Sec61/SecY plug" evidence="14">
        <location>
            <begin position="45"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF10559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GBH33722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="476" mass="51847" checksum="3F47D13BF4645BB0" modified="2018-07-18" version="1">MAEGSITTIIRKVVFKAEPYLPQVPKPKKKIPLPTRLLWCGVALLIYMIMGQTPLFGATAPQFDFLAFARVIFASQQGTLVELGIGPIVTAGLLMQLLRGSDILKFDFKKPEERGIFQTATKLVTYIVIVAESIVYATAVYGPGVTEPYFLYVMIGQLMAASIIIMFLDELIQKGWGLGSGISLFIMAGVAQQILWSMFSPLPAGDGGTIGIIPYLSQSILNGDLSNVFFRQNQLPSIFGVFLTAGILLILVFTQGMKIEIPIVSTKYRGFSAVYPIKLMYVSNIPVILASALTANAVFIFQMLWAHMNPRNNNFFMNFIAQFDPTSPSTPVGGIIYYITPPRGLDVAALDPMRAVGYILFMVGIVVVFGRLWVELGGLSPKSAAKNLLDADVQIPGFRRSNAPVEALLNKYIPSVTIIGSMILGLLAGVSDVLGVFGSGIGILLMVDILINYYTQLVKEQVEVVMPRLGALLGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPZ3</accession>
    <name>A0A2S2KPZ3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Exosome complex component Rrp41</fullName>
            <ecNumber evidence="8">3.1.13.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rrp41</name>
        <name evidence="11" type="ORF">NZNM25_04650</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalytic component of the exosome, which is a complex involved in RNA degradation. Has 3'-&gt;5' exoribonuclease activity. Can also synthesize heteropolymeric RNA-tails.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7 8">Component of the archaeal exosome complex. Forms a hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers. The hexameric ring associates with a trimer of Rrp4 and/or Csl4 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the RNase PH family. Rrp41 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.-" evidence="8"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876335.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPZ3"/>
    <dbReference type="GeneID" id="76209817"/>
    <dbReference type="OrthoDB" id="24266at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11366">
        <property type="entry name" value="RNase_PH_archRRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000004">
        <property type="entry name" value="Exosome complex component Rrp41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00591">
        <property type="entry name" value="Exosome_Rrp41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011807">
        <property type="entry name" value="Rrp41"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02065">
        <property type="entry name" value="ECX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF0">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8 11" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 8" id="KW-0271">Exosome</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="9">
        <location>
            <begin position="26"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="10">
        <location>
            <begin position="157"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH33674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="244" mass="26445" checksum="871F652026F8ADEC" modified="2018-07-18" version="1">MGGRDATMVLLDENGIRCDGRKVDEPRRIMIKAGGLKNADGSAYIEFGDNKILVGVFGPRDVHPKHMSNTDTGILRVRYHMEPFSVGERKNPAPSRREIEISKVIKEALEPAVMLDKFPRTAVDVFIEVLQADGGTRCAALTAASVALADAGIPMRDMVAAIAAGKVADTVILDVNNEEDQAGQADMPIGYMPNLEKITLLQLDGVLTPEEYKKCVQVGVEGCKLVYDLQKKALQDKYFGNGAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ61</accession>
    <name>A0A2S2KQ61_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Malate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_05800</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the LDH/MDH superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876434.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ61"/>
    <dbReference type="GeneID" id="76209932"/>
    <dbReference type="OrthoDB" id="2596at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004459">
        <property type="term" value="F:L-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006089">
        <property type="term" value="P:lactate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01339">
        <property type="entry name" value="LDH-like_MDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000018">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011275">
        <property type="entry name" value="Malate_DH_type3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004863">
        <property type="entry name" value="PRK06223.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128">
        <property type="entry name" value="L-2-HYDROXYCARBOXYLATE DEHYDROGENASE (NAD(P)(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128:SF16">
        <property type="entry name" value="L-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00086">
        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0520">NAD</keyword>
    <keyword evidence="4 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="10">
        <location>
            <begin position="2"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="11">
        <location>
            <begin position="145"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="6"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="116"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH33789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="304" mass="32811" checksum="3052455C2C515B19" modified="2018-07-18" version="1">MITIIGAGKVGGDAALFSALKKLDDQILLLDVVEGLPQGEAMDINHMLSEQGIDVEVKGSNDFADMKGSNIVVVVAGSGRKPGMTRMDLLKINATIVKSVVENVKKYADNSMIIPVTNPLDPMAYITYKVSGFDRSRVFGMGGMLDLSRFRQFIHEATGHSRDSIRALVIGEHGENMLPLPRFSSVSGIPLSSFLPKEKLDELIQNTKQVAAKVIELKGATVHAPGNAISAIVESVMRDRKQVIPVATYLDGEYGHSDVTIGVPAVIGRKGVEKIIELDLNDEEKQIFDKAVESVKSAISGIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ92</accession>
    <name>A0A2S2KQ92_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_06270</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876475.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ92"/>
    <dbReference type="GeneID" id="76209984"/>
    <dbReference type="OrthoDB" id="29144at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00445">
        <property type="entry name" value="NDH1_NuoN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010096">
        <property type="entry name" value="NADH-Q_OxRdtase_suN/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003918">
        <property type="entry name" value="NADH_UbQ_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01770">
        <property type="entry name" value="NDH_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22773">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01437">
        <property type="entry name" value="NUOXDRDTASE4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="162"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="205"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="255"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="413"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="456"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="6">
        <location>
            <begin position="127"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="494" mass="52665" checksum="8E6FB0E31B98E1C9" modified="2018-07-18" version="1">MLEITSTPLVLIAILGTVGVILPIISIARKEKGSNSFYAVIAFAALIVSLGYVGYQFINDNVSTSALFSEDVLVDDGFGGFFAIAMLIVALFTTVGSFNYMRKHNSPAVYYSLILLATIGMVLVAYSTDLVMLFVAWELMSIPTYVLVGFMKKNPSSNEAALKYFLFGALSSAIIVYGISISYGLTGSTNIGEVIQGYSALDPSLLPLALLSVGMFIAGFGFKMGLVPFHQWLPDTYEGAPSPITALLAAATKKAGFAATIRIVVLGMVVLNLDWTLALGIIAVMTMTIGNVAAIMQKNLSRMLAYSSIAHAGYILIGLAVAPHSSLGLQGSLYQIMNHAVMKGAAFIAIAGIVTTLAVTNIDKLKGLGRRMPITALGLVIALFALAGIPPLSGFWSKLMLFGSALDASSALWWAPWLAIAGVLNSALSLAYYGWITRKMYFEGESEKRVAEPKSIIAVMIFSTIFLVGFGVYPDPLIKFVEFATPVISLGLMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQE7</accession>
    <name>A0A2S2KQE7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">50S ribosomal protein L18e</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_04690</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876338.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQE7"/>
    <dbReference type="GeneID" id="76209821"/>
    <dbReference type="OrthoDB" id="11309at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_uL15_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003079">
        <property type="entry name" value="PRK04005.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="115" mass="12448" checksum="F398AECE1BD78B52" modified="2018-07-18" version="1">MTNQVVIQMAKDLKKASTKNDAPIWAKLAEYALKPSIARRDLNLNRIGQLTKENDIVVFPGKVLGTGIVPHKITLFSFSISNTAASKIVEKGGKIISYSDLIEQNPTGKGVVLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQG6</accession>
    <name>A0A2S2KQG6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_05620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876418.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQG6"/>
    <dbReference type="GeneID" id="76209913"/>
    <dbReference type="OrthoDB" id="5972at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="106" mass="12474" checksum="AF10B0180D66A018" modified="2018-07-18" version="1">MLSEKGKHASATQNRRWVWSEIIWPLVLEVNDVSFTLKQFQNKRQKICQEQNISINVPSRGLVSLMQKGILLKEGEIYSIHYRLIPYMRLKAECDYSTAIHEVRIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQJ0</accession>
    <name>A0A2S2KQJ0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CheC-like protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_07170</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876583.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQJ0"/>
    <dbReference type="GeneID" id="76208958"/>
    <dbReference type="OrthoDB" id="182374at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17905">
        <property type="entry name" value="CheC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1550.10">
        <property type="entry name" value="CheC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007597">
        <property type="entry name" value="CheC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028976">
        <property type="entry name" value="CheC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038756">
        <property type="entry name" value="CheX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39452">
        <property type="entry name" value="CHEY-P PHOSPHATASE CHEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39452:SF1">
        <property type="entry name" value="CHEY-P PHOSPHATASE CHEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04509">
        <property type="entry name" value="CheC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103039">
        <property type="entry name" value="CheC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheC-like protein" evidence="2">
        <location>
            <begin position="102"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="226" mass="25379" checksum="0B3FEAEBE25BF114" modified="2018-07-18" version="1">MNLQKIEIKKLSSTFNDYITEKTCEVFSTLLKEPIQHKLTILENNVNVRNICLTSKQIKLHSVRLNGKGDIHIELLYTLRLEDAMKIASKLLGSEVNMIDEMGESALQEVANILTGSFFNALSKDTGFRIDLSTPTFKEGELEELLSEPTNDVNSNSEDIVIADVLLTGKNSKTEIHMIIIQHPEHAKKLISHDSKDIEENKYSDKSNTNLLGGQNDAIDDLLKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQJ5</accession>
    <name>A0A2S2KQJ5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutamine--fructose-6-phosphate aminotransferase [isomerizing]</fullName>
            <ecNumber evidence="2">2.6.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">NZNM25_05920</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876443.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQJ5"/>
    <dbReference type="GeneID" id="76209944"/>
    <dbReference type="OrthoDB" id="8692at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006047">
        <property type="term" value="P:UDP-N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00714">
        <property type="entry name" value="GFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000006">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005855">
        <property type="entry name" value="GFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047084">
        <property type="entry name" value="GFAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01135">
        <property type="entry name" value="glmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001484">
        <property type="entry name" value="PRK00331.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937">
        <property type="entry name" value="GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE, ISOMERIZING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="GLUTAMINE--FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 11" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="9">
        <location>
            <begin position="2"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="10">
        <location>
            <begin position="277"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="10">
        <location>
            <begin position="439"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH33801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="586" mass="65122" checksum="115EA140275B5278" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQK7</accession>
    <name>A0A2S2KQK7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Transcription elongation factor Spt5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">spt5</name>
        <name evidence="8" type="ORF">NZNM25_05290</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Stimulates transcription elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterodimer composed of Spt4 and Spt5. Interacts with RNA polymerase (RNAP).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPT5 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the archaeal Spt5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876390.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQK7"/>
    <dbReference type="GeneID" id="76209880"/>
    <dbReference type="OrthoDB" id="371863at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006354">
        <property type="term" value="P:DNA-templated transcription elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06091">
        <property type="entry name" value="KOW_NusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09887">
        <property type="entry name" value="NGN_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="entry name" value="NusG, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00950">
        <property type="entry name" value="Spt5_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005100">
        <property type="entry name" value="NGN-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006645">
        <property type="entry name" value="NGN-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036735">
        <property type="entry name" value="NGN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011590">
        <property type="entry name" value="Spt5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00405">
        <property type="entry name" value="KOW_elon_Spt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03439">
        <property type="entry name" value="Spt5-NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00738">
        <property type="entry name" value="NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NusG-like N-terminal" evidence="6">
        <location>
            <begin position="6"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="7">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="NCBIfam" id="TIGR00405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="152" mass="16721" checksum="596ABE0854FD843E" modified="2018-07-18" version="1">MSEEIKSHLFAIRTTGGQEKVVMRLLEAKANANQINIQSVLLVDNLKGYVVIEAINPSDAYMAVEGVRHIRGQLRGELEFKDIEGYLIKKSTVSQLAVDNIVEITGGPFKGMKATITRIDVDKEEATVVLLDASYQLPVTVDANYLKISSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQK8</accession>
    <name>A0A2S2KQK8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07610</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876621.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQK8"/>
    <dbReference type="GeneID" id="76208915"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="250" mass="30372" checksum="385E7D6E57B49C33" modified="2018-07-18" version="1">MLGIFHLKKFKFEFKDEIFYSFDKNFIPHLLGKRGNQTAINTAGSTWKKEIQRYLNQRQAENRSQNPKWTELFNFLQGHLTTESALFLPHKDKIIKEDIKEIFQSTNRKDKERLGEFLRFREILRYNLIFFKKESKFRHKILPMIFDENNDFRRDVSPQYLYILLSLYVARKYSIFSNYKYKEFANKLKIGRDTYNKKFLDGLKSSQSIHNLTALCYTQYFWFDYRLSQSEHSEIHKILFNMFNELNLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQK9</accession>
    <name>A0A2S2KQK9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_07300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146195978.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQK9"/>
    <dbReference type="GeneID" id="76208945"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="124" mass="14030" checksum="944FEE3B8E18D618" modified="2018-07-18" version="1">MNVKVISAMVLVTTLLLAQTVSIVDAHHNPDSRNENARKEIEEKSKDYDKQGNKKEVIEERKKAFLAYKTAFASWKTAKDDYKSAKRSGDQTVIDAKKVILDAAKIVKDDAFAEYKEALKQKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQM3</accession>
    <name>A0A2S2KQM3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Archease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_07050</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876538.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQM3"/>
    <dbReference type="GeneID" id="76210066"/>
    <dbReference type="OrthoDB" id="8831at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.10.10">
        <property type="entry name" value="Archease domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002804">
        <property type="entry name" value="Archease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023572">
        <property type="entry name" value="Archease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036820">
        <property type="entry name" value="Archease_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12682">
        <property type="entry name" value="ARCHEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12682:SF11">
        <property type="entry name" value="PROTEIN ARCHEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01951">
        <property type="entry name" value="Archease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69819">
        <property type="entry name" value="MTH1598-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Archease" evidence="5">
        <location>
            <begin position="3"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="138" mass="15974" checksum="58F928F89217CBFF" modified="2018-07-18" version="1">MSYKFLDHATDAIIEVTAKDIKEAFAVAADAEINLTLDQDKVEEKDKKEFSANGKDLRYLLFSWLEEIPFLLITEGFAIKRIEFDIEENNGYKISATAFGEPLDVHKHNFKVEIKAPTFYDMEIRTNGQVYMRFLLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQS5</accession>
    <name>A0A2S2KQS5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_08130</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutaredoxin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioredoxin family. DsbA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQS5"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13887:SF14">
        <property type="entry name" value="DISULFIDE BOND FORMATION PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13887">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13462">
        <property type="entry name" value="Thioredoxin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="73"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="269" mass="30181" checksum="B199615F8E2554F7" modified="2018-07-18" version="1">MELIKMSSNSEFNENSKNITVKKSTFNLLIIGLIVIVGIAAFFAGSYVSNSNQITDEELEEAIAKLELKVLQNQLPTKQPSQQVKISADNDPIIGNPDAPITIIEFSDFQCPFCARFHTQTLPLILEQYIEQGKVKLVFRDFPIQSIHPNALPASVAAECANEQGKFREMHDILFDNQNQWNKQETVDALDLFSDYAMEIQLNQEIFDSCLTSGKYIEEIRKDLDDGRDYGVSGTPGFFVGNEKIGFVELKGAQPFDSFKKIIDAQLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQU8</accession>
    <name>A0A2S2KQU8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Inorganic phosphate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_08510</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Potential transporter for phosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876698.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQU8"/>
    <dbReference type="GeneID" id="76208820"/>
    <dbReference type="OrthoDB" id="101311at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101:SF80">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="105"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="133"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="256"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="328" mass="34095" checksum="CC36C45F71BAD4D8" modified="2018-07-18" version="1">MIEIAIGAIIVALIFDFVNGFNDSANSVATVIGTRVLKPIHAVGLSAAMNFIGPFIFGVAVATTIAKGIVSPDDITVYMIIGGLAGAIGWSTLCTYFGLPISNSHSLVGGIMGAGIAGLGFEKLVYGGLTKIFAGIVISPVGGMIFGLLVTTLIITVFAGYKPGPVNKTFGKLQIVSSAWFALTHGANDGQKTMGIIVLILFSAGMIPELEMPLWVILAAATAMGLGTFFGGYKVIKTLGVKITKLKPYQGFAAETSGGLMLAIFAVLGIPASTTHAITGTIMGSGAARRKRAVRWKVSRQIVFSWIITIPGSALMGMGVTYLIQLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQV2</accession>
    <name>A0A2S2KQV2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_160049197.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQV2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="55" mass="6306" checksum="B20E5D44F76FBD83" modified="2018-07-18" version="1">MANHIIHFAVSIKNPISGQENAGSCEFEKLASFRKLNILDYDRSFKKYFLEIGIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQY0</accession>
    <name>A0A2S2KQY0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphate transport regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0111 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876697.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQY0"/>
    <dbReference type="GeneID" id="76208821"/>
    <dbReference type="OrthoDB" id="145921at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018445">
        <property type="entry name" value="Put_Phosphate_transp_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052912">
        <property type="entry name" value="UPF0111_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37298">
        <property type="entry name" value="UPF0111 PROTEIN YKAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37298:SF1">
        <property type="entry name" value="UPF0111 PROTEIN YKAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01865">
        <property type="entry name" value="PhoU_div"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109755">
        <property type="entry name" value="PhoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="209" mass="24192" checksum="671740D113CDB939" modified="2018-07-18" version="1">MGQWLSWIKSNEKELLKILDDLAKKAVETSEAVVVLFNDLSNKEQAKKIHQLETEADVLTRDIFSELNKTFITPLDREDMQRIASKIDDVIDFMDGIAARVYSYQITTPPPYTAEMAHELVKATKEVEYMISKLQRIKNPKDMIEHCRNTSDIEHTVDDLYREAIKELFETNDAIKIIKLKDIYETMETASDRCVDVADVIEDIVLKYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQZ7</accession>
    <name>A0A2S2KQZ7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lyase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQZ7"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051344">
        <property type="entry name" value="Vgb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40274">
        <property type="entry name" value="VIRGINIAMYCIN B LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40274:SF3">
        <property type="entry name" value="VIRGINIAMYCIN B LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="512" mass="56901" checksum="207271532759A09D" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR02</accession>
    <name>A0A2S2KR02_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_07520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876612.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR02"/>
    <dbReference type="GeneID" id="76208924"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="134" mass="15152" checksum="9878C6CE162D7FAD" modified="2018-07-18" version="1">MFENSTMIIIVPIISASIAAAGLIFTGLSYQNNTKAKYLQVLREFDNEISELEMSQTRVSNYDQFVARYLNIHERLAFLAQNRKIPGDLANYYDSSFAASLGILQMPKYQHHATDLDNLTCWCKMNGIEPGNPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR34</accession>
    <name>A0A2S2KR34_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Oligoendopeptidase F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pepF</name>
        <name evidence="9" type="ORF">NZNM25_08080</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876889.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR34"/>
    <dbReference type="GeneID" id="76208866"/>
    <dbReference type="OrthoDB" id="275607at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09610">
        <property type="entry name" value="M3B_PepF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.20">
        <property type="entry name" value="Oligoendopeptidase f, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.70">
        <property type="entry name" value="Oligopeptidase f, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013647">
        <property type="entry name" value="OligopepF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042088">
        <property type="entry name" value="OligoPept_F_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011977">
        <property type="entry name" value="Pept_M3B_clade3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02290">
        <property type="entry name" value="M3_fam_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804:SF5">
        <property type="entry name" value="OLIGOENDOPEPTIDASE F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08439">
        <property type="entry name" value="Peptidase_M3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Oligopeptidase F N-terminal" evidence="8">
        <location>
            <begin position="115"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M3A/M3B catalytic" evidence="7">
        <location>
            <begin position="198"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="590" mass="68084" checksum="5105B18E939F0BB2" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR45</accession>
    <name>A0A2S2KR45_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048115766.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR45"/>
    <dbReference type="GeneID" id="24819961"/>
    <dbReference type="OrthoDB" id="10281at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="77" mass="8912" checksum="FFEBC28D6830E4C8" modified="2018-07-18" version="1">MDKHKPSDEMIKELDNLLSKINAMEIVASDDYQKNSIKIMRALVEGQMHSINEFQHLKKAIDLLTLQLFDVQNKVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR90</accession>
    <name>A0A2S2KR90_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitrogen regulatory protein P-II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">glnB_2</name>
        <name evidence="2" type="ORF">NZNM25_09770</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the P(II) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876806.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR90"/>
    <dbReference type="GeneID" id="76208692"/>
    <dbReference type="OrthoDB" id="10960at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017918">
        <property type="entry name" value="N-reg_PII_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30115">
        <property type="entry name" value="NITROGEN REGULATORY PROTEIN P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30115:SF11">
        <property type="entry name" value="NITROGEN REGULATORY PROTEIN P-II HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00340">
        <property type="entry name" value="PIIGLNB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00938">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00638">
        <property type="entry name" value="PII_GLNB_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="104" mass="10884" checksum="350C05F8423D1F9E" modified="2018-07-18" version="1">MKKIEAIIQSGAKDAVVAAIKKIGVGGITVHQVQGQGSQDPPLVGQYFSRDMIICVVDDPKVDEILDAIANVACTGTKGDGKVFVTPVDDALDICTKKRGTTSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR92</accession>
    <name>A0A2S2KR92_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_08780</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876716.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR92"/>
    <dbReference type="GeneID" id="76208792"/>
    <dbReference type="OrthoDB" id="4984at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF8">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="52"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="131" mass="15235" checksum="5448D9349CA6A73F" modified="2018-07-18" version="1">MIILVGLFTPKDPQKKKNIEKLKEIKKLVKEKKYVAALKSGTEYLHKVPHHHDLLFTLGGIYYLQKKYKTAITYFERALEIGSYDIEVLLLKAYSHQKLGENKRSIQCCEKIQEINPKNKAVIALLEELNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRA8</accession>
    <name>A0A2S2KRA8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_09870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876814.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRA8"/>
    <dbReference type="GeneID" id="76208680"/>
    <dbReference type="OrthoDB" id="11953at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="344" mass="37366" checksum="97F45E96B5AF9FF7" modified="2018-07-18" version="1">MTKFIVAILMITLLGSILQTWGGSWDITSHQLGEPETFFTPPHGVLYIGVGIGLIAAIMSVAVMLKKREILKKSFSLGLKLIIIGCVFQIIAGPSDYYWHEMFGTDGLLSPTHLTLITGILIQSVGIVIGLARLIHKNFRVVKPSLILAFGVLWFITIGFIFQFTLPISTGDTIDLDPDPYIAAVIAASALPFFSVLIFWSSAKTIGRFGGATLVTVVFIILNITSNIIPAEFLWGFLPWFALPVILGIVSDALISSKIKIKRFGEELAGAIIGSMFLVFSMPLIGMAYIQFHIFNGVSGYELLPDFADTLGMILGIMSIPGAIMGWVAVKFAKRKIAIPAELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRC9</accession>
    <name>A0A2S2KRC9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_10070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876828.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRC9"/>
    <dbReference type="GeneID" id="76208661"/>
    <dbReference type="OrthoDB" id="382318at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="113" mass="12873" checksum="868985D168C3DE64" modified="2018-07-18" version="1">MRYWKIILIKLRGGVFEITKTVLAVAVMVVLIAGVIAPAFQDVHAYTQKAKFSSEKLKAKSYGIKTKDKVSFDEKETKHNSFGEIKKQDVKNYKKIMAEYSAKKILKNLYNLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRF8</accession>
    <name>A0A2S2KRF8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_09850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRF8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="121"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="201" mass="21915" checksum="52A4BF699D5B17E1" modified="2018-07-18" version="1">MLNQIRGGEYKIMKVKLTVFVAVLTASLLLAGPSHAFAASSPSSRILAIVPTSVENIHSVIFEVCASDTVNMRAPEVIINSLAEVKNVKLSKVIAKGTCTTNAAQLSAFDPANIKIKVIDKSKLNTMVDQAEKNLIKIKSDISKTNAELQGLLDNIPGNTPTKKDDIAKINDLTSKLSELRMQLKDVRSEYHRLLYILKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRL5</accession>
    <name>A0A2S2KRL5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutamate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_09720</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homohexamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 8">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876906.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRL5"/>
    <dbReference type="GeneID" id="76208697"/>
    <dbReference type="OrthoDB" id="6425at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014362">
        <property type="entry name" value="Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF13">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000185">
        <property type="entry name" value="Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="191"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="7">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="424" mass="46346" checksum="5A7DA0ED0F56311B" modified="2018-07-18" version="1">MVKNDPFANATKQVNDACDLLGIKDPGLREYLAMPNRVLRVKIPVRMDNGKIRVFTGFRSQHNNDRGPYKGGIRYFNPEGGVEYMEREVMALSSWMTWKCAIVDVPLGGGKGGIYVNPKTEKISEAELERLTRGFAFKIFEVIGPGKDIPAPDVYTTGKEMTQIMDTYSKLTGNIYSPGVITGKPISMGGSLARNVATGLGAAYCVREAAKTMKLSLKGAKVVLQGFGNASTFAGEYLEKMGAKIIGVSDSKGSILISSGAKVSKIIEYKQKHGSVVGFPGSKKVSTEELLTTKCDVLVPGALENQIDAKIAKNLKCKIIAEAANGPTLPEADPIIFQKKILVIPDILANSGGVCISYLEWVQNNMGYYWTFDEVANKMEKNITKGFKDAYEMSKKHKVDMRKATMALAVQRVVEAFNEKGIWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRL7</accession>
    <name>A0A2S2KRL7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hsp20/alpha crystallin family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_10840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877019.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRL7"/>
    <dbReference type="GeneID" id="76208575"/>
    <dbReference type="OrthoDB" id="10797at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="17287" checksum="977D048564AC559F" modified="2018-07-18" version="1">MASYKGAYSNEQSLNFVIPIIVILFLGIIYIMTQRADSGFVSFILIGAAALTMFYWVRVLKKMTKDQKPLYTQAREQETKNWVYDLIKGEGEFVFVAEVPGPEDKIAVRLVDGILYIRGTAGFSKEVPIEGANEMQIFDFKYRNGVLTLRIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRN2</accession>
    <name>A0A2S2KRN2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HEAT repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_09060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRN2"/>
    <dbReference type="OrthoDB" id="142930at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697:SF5">
        <property type="entry name" value="DEOXYHYPUSINE HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697">
        <property type="entry name" value="PBS LYASE HEAT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="17895" checksum="2A586A31907452F0" modified="2018-07-18" version="1">MQAVTDERLALFAEMESKYEQKDTDYFVSLLEHPDYVVRTRVACILVDFGGEDKVQYIAKVLKNDDNELVRHEAAFALGQMSYSSAIPPLTDATLNDPSMFVRHEAAIALGVVGNKDAKEALQKALNDPDLPVVESAVVALSNIEFMEKLSKNEQFAKLTGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRR3</accession>
    <name>A0A2S2KRR3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876981.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRR3"/>
    <dbReference type="GeneID" id="76208520"/>
    <dbReference type="OrthoDB" id="4898at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="177" mass="19843" checksum="288B186FC8B4F90A" modified="2018-07-18" version="1">MIKPIYFALGAIPVIVALLISVPLLTKNEIPISAAHSFDKIDIEYTKHQLKKISYGITERVGAQKTEILSIKNDGDVKYSVTEHGYIQPDIRSKLDKDKLDKLKALIKETGFIAIPSESFSILENVTEYQKSNVKITLNGHVNQIHWPEQNATSDFIPPIITMVESELDQIISQIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRW8</accession>
    <name>A0A2S2KRW8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRW8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="282" mass="33103" checksum="E10BCF0D120033C4" modified="2018-07-18" version="1">MLFFVRLGTRSPNEFIQLLNQRNDTIQKKCVKKISELAEMIDTKVMLGDSTITGQKTFDPKLVTDYFQKINDSLEDWSVQDVSISNNEDLRRVFTKFEIMEGSYLISGHISLQYHVLLYYKPDQRVIDCQKELADIVDITKNKEKELSDNSDQFVLNKLKEMGYKDFDHQKLFEVFYENDEFREKVYAEIEKDAGMDFKELSEKKRKLFNELDSLLIETYQTSPVLIDDARLVSGEEGCLCTIDLEFVKNEIKEGLFDPRKMSDSVKEKIIKRLDEFEKILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRX2</accession>
    <name>A0A2S2KRX2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_09890</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRX2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="260" mass="29853" checksum="BD87E7DB3D10F410" modified="2018-07-18" version="1">MVIVYTYPELFGLPRPYSKIGEYDGAVSETALMINEQCLNKDQFSDYPFTKTSNGHYYIDNVICERINVEQGGCLEPFSKGFPDETCQNRVTFDQPYGETGPYFNKEFCNHVKFWEPALTDTVENKRVNSEWLHICTIRGLIADTPLHDISQIMNYQGKNCNVYRFGSVDCFAISFDKCIPAKIENTHFTVEGDPITVIAMIKSDSCSIDVFHDATQDRFGKQEITKYSCPEIKLDDNYLHMVSCVDEFDEGEYGFIIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRX5</accession>
    <name>A0A2S2KRX5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ornithine cyclodeaminase</fullName>
            <ecNumber evidence="8">4.3.1.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Archaeal ornithine cyclodeaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">NZNM25_12210</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the conversion of ornithine to proline, with the release of ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-ornithine = L-proline + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:24368"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="4.3.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the AgrE/ArgZ ornithine cyclodeaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.12" evidence="8"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877068.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRX5"/>
    <dbReference type="GeneID" id="76209283"/>
    <dbReference type="OrthoDB" id="64170at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008473">
        <property type="term" value="F:ornithine cyclodeaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd12144">
        <property type="entry name" value="SDH_N_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2690">
        <property type="entry name" value="LOR/SDH bifunctional enzyme, conserved domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10690">
        <property type="entry name" value="putative lor/sdh protein like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005239">
        <property type="entry name" value="ArgZ/ArgE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048964">
        <property type="entry name" value="ArgZ/ArgE-like_C_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048963">
        <property type="entry name" value="ArgZ/ArgE-like_C_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007545">
        <property type="entry name" value="LOR/SDH_bifunc_enz_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043009">
        <property type="entry name" value="LOR/SDH_bifunc_enz_cons_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00300">
        <property type="entry name" value="TIGR00300 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21571">
        <property type="entry name" value="ArgZ-like_C_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21570">
        <property type="entry name" value="ArgZ-like_C_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04455">
        <property type="entry name" value="Saccharop_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LOR/SDH bifunctional enzyme conserved" evidence="11">
        <location>
            <begin position="5"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Arginine dihydrolase ArgZ/ArgE-like C-terminal first subdomain" evidence="13">
        <location>
            <begin position="105"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Arginine dihydrolase ArgZ/ArgE-like C-terminal second subdomain" evidence="12">
        <location>
            <begin position="187"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00052109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00081581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF21571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GBH34430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="410" mass="45694" checksum="0E84BC0145E0D894" modified="2018-07-18" version="1">MSKFSQEIEVSGHLIDSSILTKIFDKIMDLHGEFQVEEINIGKRKKDQSYVRLLVKGKNQKHLDEILETVYRLGAVSKIQKEITLKKSPKNYVMPDDFYSTTNNHTQVFYKKRWIQVENMMMDKCIVVKQNRAFCVPVRDVKKGDQIIVGEEGIKITPPERPREGINVFEFMGSSSSSERPTQHIAKQVADDIYDTKKNGGKIVIVGGPAIVHTGADDSVSELIRLGYIDGVLAGNALAVHDIEYATLGTSLGMNVHNATLAYHGHRNHMDTINAVFKAGSIANMVKTKKLKKGIMYECVKNKVPFVLAGSIRDDGPLPDVITDVAQAQREYKKIVKDASMVIMISTMLHSIATGNMLPANVKVIVVDINQPTVTKLMDRGTWQALGIVSDVGAFLPLVAHQLRKKKKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS64</accession>
    <name>A0A2S2KS64_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_11820</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877034.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS64"/>
    <dbReference type="GeneID" id="76209324"/>
    <dbReference type="OrthoDB" id="43988at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06423">
        <property type="entry name" value="CESA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43630">
        <property type="entry name" value="POLY-BETA-1,6-N-ACETYL-D-GLUCOSAMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43630:SF1">
        <property type="entry name" value="POLY-BETA-1,6-N-ACETYL-D-GLUCOSAMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="179"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="216"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="527"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="558"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="591"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="4">
        <location>
            <begin position="285"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="635" mass="72091" checksum="160E0AAB78B57B00" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS72</accession>
    <name>A0A2S2KS72_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12840</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829083.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS72"/>
    <dbReference type="GeneID" id="76209216"/>
    <dbReference type="OrthoDB" id="2634at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="432" mass="49611" checksum="EA54B85C2BDE1C34" modified="2018-07-18" version="1">MGLSNQNLLGLKLFSPSSINDKSLEFFEFLKYETKIFRNDSTILKKNLINEIPKSLKIKQTSRKQQTLKKFEKFLQNLDNILFIKISDDEISEIKWDDIDDFCGLLRLIERSDERKVRWALYHLFAKGHLEISKTQLAESLKEFKIKHFDKILAKIIKTETLSGLDAEFNGTTFIINHDAGDKLVSHYLADAIEEQSRRSPGELEEEILKLIDEGSYSNQEISRSLLIDEGLVSRTISKLRDQDKIILSSFGQRGARYFTTNCDNCPFGTTKTSCRKEALSFIISSFMKDFEVNLSANDFDSVESNQALLKIKRIIMMARKEKNTKLERNLSKNLDDILSKVVEKFIKIEFPDKSSGGSKINFGVSSAISNLPILYQLGLRKGAHEGIRLMNELLLESKTIQKEDRVKIQKHAMKEANKFLKNLGLEYNKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSD8</accession>
    <name>A0A2S2KSD8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_13250</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSD8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="878"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="436"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="441"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="915" mass="99419" checksum="17F2ADEDD91C9524" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSE4</accession>
    <name>A0A2S2KSE4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methylmalonyl-CoA epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_13570</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methylmalonyl-CoA epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877173.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSE4"/>
    <dbReference type="GeneID" id="76209142"/>
    <dbReference type="OrthoDB" id="6161at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004493">
        <property type="term" value="F:methylmalonyl-CoA epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046491">
        <property type="term" value="P:L-methylmalonyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07249">
        <property type="entry name" value="MMCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.180.10:FF:000044">
        <property type="entry name" value="Methylmalonyl-CoA-ethylmalonyl-CoA epimerase protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017515">
        <property type="entry name" value="MeMalonyl-CoA_epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051785">
        <property type="entry name" value="MMCE/EMCE_epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03081">
        <property type="entry name" value="metmalonyl_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43048">
        <property type="entry name" value="METHYLMALONYL-COA EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43048:SF3">
        <property type="entry name" value="METHYLMALONYL-COA EPIMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13669">
        <property type="entry name" value="Glyoxalase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="3">
        <location>
            <begin position="2"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="132" mass="14467" checksum="B45D9E08A6085528" modified="2018-07-18" version="1">MKIDHIAIAVNDVEESAKVYQQALGVDNVEFETVETEGVKVAIIHLENGRVELMQPTNDSSPIKKFLDKKGPGLHHMALETDNIEGEVERMEGCGIQFLGQIRPGSAGTKVTFIHPKSLHGVLAELCSHPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSG7</accession>
    <name>A0A2S2KSG7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Group-specific protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_13840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877197.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSG7"/>
    <dbReference type="GeneID" id="76209115"/>
    <dbReference type="OrthoDB" id="879at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="94" mass="11107" checksum="93F0543F234AFFBE" modified="2018-07-18" version="1">MNVWNKFWKWYEDKILGSIIIIAIIQFIQVPHMVWNADIMLEAGLVSRIHPVIDWFLYGVDLIEIISIINVGMIMFALIKKRRASKNQPKIIER</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSK1</accession>
    <name>A0A2S2KSK1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866890.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSK1"/>
    <dbReference type="GeneID" id="76209170"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="581" mass="61334" checksum="FEA26501B2BA2CD7" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSK7</accession>
    <name>A0A2S2KSK7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ArnR1-like winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_14200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829089.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSK7"/>
    <dbReference type="GeneID" id="76209075"/>
    <dbReference type="OrthoDB" id="9844at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="9718" checksum="368E70F8A3431B1C" modified="2018-07-18" version="1">MKLYEYGEMNQSRLMSICGLNNVKHKEILDSMVNKQMIERKEEPWGNKTIYKYKISEKGKTLLEEVLQRYEDLFPRDNGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSN9</accession>
    <name>A0A2S2KSN9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12660</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877103.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSN9"/>
    <dbReference type="GeneID" id="76209236"/>
    <dbReference type="OrthoDB" id="4806at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="76" mass="8802" checksum="DAAFCE1FA15528A7" modified="2018-07-18" version="1">MTENNDLSEHDLFVTEFPKKDFGIVDYLQGRVHFALLDQMKFMSASGMTKTQIKEVILTNVTEIIDKLEEKESESF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSQ6</accession>
    <name>A0A2S2KSQ6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator TrmB N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_14930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877289.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSQ6"/>
    <dbReference type="GeneID" id="76209002"/>
    <dbReference type="OrthoDB" id="30795at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051797">
        <property type="entry name" value="TrmB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="1">
        <location>
            <begin position="11"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="386" mass="43244" checksum="4862A2DC754BFA8F" modified="2018-07-18" version="1">MVDEHVLTVSLEEFGLSKYEAQAYVALISKGTITASELAYYSEIPRTKIYPTLLKLENKKLAIISKSKPIMCTAIAPEDAFDGIIHEQINKVNAMNSLVSNLKKASEESRKTRGSEEKRYFHISANNVLLQLQTMIEGSKSSIKIMTDQWGFGLLAECKDQLSSVVRRNLDIKIIVPTTQICSESHRKIPDRVDIRASEITQNCFIFDETELLLINNDNGKGAIFSSTDILGSNQEKIFSNLWKNSIKTKALADMSKTEAQEIYKIIKTVNEIGLTYILNSAMVSKKPESDMFKLLENNGISFKSKSLDDVIEIMDAIMQITCSGHVNFEANTKNITVESKLNSGHSLPWVSILDGCLQKQGYKTRTIYQNNANKGEKVHIKISKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSR8</accession>
    <name>A0A2S2KSR8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12860</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829084.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSR8"/>
    <dbReference type="GeneID" id="76209214"/>
    <dbReference type="OrthoDB" id="1960at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="60" mass="6652" checksum="C042499091CE0776" modified="2018-07-18" version="1">MQGVNQTIMHKMHNPEKSHSSVSKVECYVCGKGLEDGRCITAKTLPNGTVLFCDVHYSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSU1</accession>
    <name>A0A2S2KSU1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_15410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877376.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000009.1"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3105at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1435"/>
            <end position="1459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1440"/>
            <end position="1449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="1722" mass="180679" checksum="587EAF9D79D56600" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSU7</accession>
    <name>A0A2S2KSU7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877134.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSU7"/>
    <dbReference type="GeneID" id="76209190"/>
    <dbReference type="OrthoDB" id="1930at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="136" mass="15248" checksum="BE1D1F22A248E636" modified="2018-07-18" version="1">MNEMTQTQINLGDYNKPQEQTKAVGIGKILGKIINIKDFRTNRGKPSPYTPRDAIGEDGLTDYNVIDTLETFEVDNHLISSFFVTPAIVKQIQRVPNYQSELASGKVFGPCKIGQKKSTKTNANYWCLLFPGEEGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSV8</accession>
    <name>A0A2S2KSV8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">HIT family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_14280</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877235.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSV8"/>
    <dbReference type="GeneID" id="76209067"/>
    <dbReference type="OrthoDB" id="26806at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46648:SF1">
        <property type="entry name" value="ADENOSINE 5'-MONOPHOSPHORAMIDASE HNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46648">
        <property type="entry name" value="HIT FAMILY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="4">
        <location>
            <begin position="4"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="2 3">
        <location>
            <begin position="94"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="1">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="135" mass="14932" checksum="17DB76D159A8DAB3" modified="2018-07-18" version="1">MDCIFCKIISGDIPAKIIKETSYSISFLDAFPLAKGHVLVIPKNHHQKIQDMSEKENSDLFSLVHSMISKVDSITGSTLLAIHNGKDAGQEVPHVHVHLVPRSKDDSAGAIHSMFSHSLKLSDSEIDEIYEKLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSW1</accession>
    <name>A0A2S2KSW1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_15240</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. BUD32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877362.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSW1"/>
    <dbReference type="GeneID" id="76208464"/>
    <dbReference type="OrthoDB" id="31344at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000951">
        <property type="entry name" value="EKC/KEOPS complex subunit BUD32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022495">
        <property type="entry name" value="Bud32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03724">
        <property type="entry name" value="arch_bud32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209:SF0">
        <property type="entry name" value="EKC_KEOPS COMPLEX SUBUNIT TP53RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209">
        <property type="entry name" value="NON-SPECIFIC SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH34733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="206" mass="23555" checksum="9FABB2E65B4E45E2" modified="2018-07-18" version="1">MKLIKKGAEADIFQTQWQNNNAILKIRKIKNYRNSLLDTKIRKQRTIKESQMLSSARTFGIPTPLVYFVNLEKSYIIMQEIPGTPVHDLSESKIIKLSKEIGKLVGILHKNGIMHGDLTTSNFILFKKTIYVIDFGLSQKTIKPEDHAVDLRLIKEILNSAHAKIMQSSWKNFLLGYKSIVGNAYHNKIVKLVSDIESRGRYAEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSW5</accession>
    <name>A0A2S2KSW5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7 8">Threonine synthase</fullName>
            <ecNumber evidence="7 8">4.2.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">thrC1</name>
        <name evidence="12" type="ORF">NZNM25_15430</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>O-phospho-L-homoserine + H2O = L-threonine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10840"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.2.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the threonine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.1" evidence="7 8"/>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSW5"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00065"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004795">
        <property type="term" value="F:threonine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01563">
        <property type="entry name" value="Thr-synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026260">
        <property type="entry name" value="Thr_Synthase_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006050">
        <property type="entry name" value="PRK08197.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF6">
        <property type="entry name" value="L-THREONINE DEHYDRATASE CATABOLIC TDCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038945">
        <property type="entry name" value="Thr_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 8 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="11">
        <location>
            <begin position="76"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="380"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="NCBIfam" id="TIGR00260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR038945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR038945-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038945-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH34752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="412" mass="44581" checksum="F550E1A6BF746AA5" modified="2018-07-18" version="1">MFCEEKLILTKTSLQCRECKKDYDNAFKYICDECFGPLDVKYDFPALPRNTFTNREHTYWRYFELLPVENKSNIVSIGAGMTPLIKADNLGKKLGLNNLYIKNDSVNPTFSFKDRPAGVAVSKAKEFGLSAVGCASTGNLASATAAHAAKAGMPCHVFAPSNIEMAKITQALSYGANYIAVDGTYDDANRIAAQIGDSKGIGIVNINMRSHYVEGSKTFAFEVAEQLDWQVPDQVIIPVGSGAMLNAICKGFEELQTVSLINDVSNMHMIAAQPHGCAPIVDAFKKNSKAVIPVENPDTIAKSLAIGDPGDGRYVLKRLAQYNGFAEECNNKEILDAILLLAQTEGIFTEPAGGVSVSVLQKMVEQGKIDANDKVVCYVTGNGLKATESIMEVLKKPDVLKADISEISAVVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSW8</accession>
    <name>A0A2S2KSW8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TIGR00725 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_14380</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877242.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSW8"/>
    <dbReference type="GeneID" id="76209058"/>
    <dbReference type="OrthoDB" id="9570at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005268">
        <property type="entry name" value="CHP00725"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041164">
        <property type="entry name" value="LDcluster4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052341">
        <property type="entry name" value="LOG_family_nucleotidases"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00725">
        <property type="entry name" value="TIGR00725 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43393">
        <property type="entry name" value="CYTOKININ RIBOSIDE 5'-MONOPHOSPHATE PHOSPHORIBOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43393:SF3">
        <property type="entry name" value="LYSINE DECARBOXYLASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18306">
        <property type="entry name" value="LDcluster4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102405">
        <property type="entry name" value="MCP/YpsA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="169" mass="17544" checksum="A6793948540FAE39" modified="2018-07-18" version="1">MVKKRQILVIGHNTSGCTPEHEKIAYDVGMEVAKSGSVLICGGLGGVMTAAAHGAKDGNGLTVGIIPQNDPAMANEFCDVVIPTGMGLARDFLNALSADGVIIIGGGSGTLSETTAAYMYKKPMVAIRNLGGSVEKFIDGFIDQRENVKIVGVDTPKEAVEKILELIAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSY1</accession>
    <name>A0A2S2KSY1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_13460</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877163.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSY1"/>
    <dbReference type="GeneID" id="76209153"/>
    <dbReference type="OrthoDB" id="52538at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000297">
        <property type="entry name" value="PPIase_PpiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050245">
        <property type="entry name" value="PrsA_foldase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47245:SF1">
        <property type="entry name" value="FOLDASE PROTEIN PRSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47245">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00639">
        <property type="entry name" value="Rotamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50198">
        <property type="entry name" value="PPIC_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0697">Rotamase</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PpiC" evidence="6">
        <location>
            <begin position="2"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="92" mass="10260" checksum="53998CA2DDB63332" modified="2018-07-18" version="1">MAIKIKCSHILVSKQSEALAILERIKKGEKFGKLAKELSIDSGSAKKDGSLGYFTKGMMVKPFEEAAFKLQNGEISEPIKTEFGYHIIKRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT67</accession>
    <name>A0A2S2KT67_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">prephenate dehydratase</fullName>
            <ecNumber evidence="2">4.2.1.51</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pheA</name>
        <name evidence="10" type="ORF">NZNM25_15620</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>prephenate + H(+) = 3-phenylpyruvate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:21648"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:18005"/>
            <dbReference type="ChEBI" id="CHEBI:29934"/>
            <dbReference type="EC" id="4.2.1.51"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-phenylalanine biosynthesis; phenylpyruvate from prephenate: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.51" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877392.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT67"/>
    <dbReference type="GeneID" id="76208425"/>
    <dbReference type="OrthoDB" id="8755at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004664">
        <property type="term" value="F:prephenate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009094">
        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04905">
        <property type="entry name" value="ACT_CM-PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13631">
        <property type="entry name" value="PBP2_Ct-PDT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000012">
        <property type="entry name" value="Prephenate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001086">
        <property type="entry name" value="Preph_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018528">
        <property type="entry name" value="Preph_deHydtase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008865">
        <property type="entry name" value="PRK11898.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21022">
        <property type="entry name" value="PREPHENATE DEHYDRATASE P PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21022:SF19">
        <property type="entry name" value="PREPHENATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00800">
        <property type="entry name" value="PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00857">
        <property type="entry name" value="PREPHENATE_DEHYDR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00858">
        <property type="entry name" value="PREPHENATE_DEHYDR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51171">
        <property type="entry name" value="PREPHENATE_DEHYDR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0584">Phenylalanine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate dehydratase" evidence="8">
        <location>
            <begin position="3"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="9">
        <location>
            <begin position="189"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="271" mass="30505" checksum="FF9EF85386356A54" modified="2018-07-18" version="1">MTNVSFQGERGAYSEAAALIFFNNEIDTVSFATFAEVLENTSSDKTEYAILPVENSLEGSVGESYDLLYSTDLNAIGEVYHRIQHCLIGIGRLDEVDTVYSHPQALGQCRKFIEEHSMKTIPSYDTAGSVKIVKELNKRNCASIASKNAAEIYEMPIISSNITNNLNNYTRFLILSKTNSPITGNDKTSIIFSIKHEPGSLFRIIENFHKNNVNLTKIESRPTKTNTWEYNFYVDFEGHKEDSKISEMLEKIKHDTLFMKVLGSYPSAKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT70</accession>
    <name>A0A2S2KT70_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_16200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PDCD5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877456.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT70"/>
    <dbReference type="GeneID" id="76208366"/>
    <dbReference type="OrthoDB" id="7912at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.140:FF:000004">
        <property type="entry name" value="DNA-binding protein PAE3044"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.140">
        <property type="entry name" value="PDCD5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002836">
        <property type="entry name" value="PDCD5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036883">
        <property type="entry name" value="PDCD5-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003268">
        <property type="entry name" value="PRK04239.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840:SF0">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01984">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015730">
        <property type="entry name" value="TFAR19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46950">
        <property type="entry name" value="Double-stranded DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="93" mass="10672" checksum="495E1E23CFD64970" modified="2018-07-18" version="1">MSFPESNESPYENTNNELSAQKEQILKQILSPEARLRLNNIKMVKSELSDLVEQYLIGMATQGKLPGQISDDQLKQILLSIQQPKRDFKINRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT94</accession>
    <name>A0A2S2KT94_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GMP synthase [glutamine-hydrolyzing]</fullName>
            <ecNumber evidence="10">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">GMP synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Glutamine amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 13" type="primary">guaA</name>
        <name evidence="13" type="ORF">NZNM25_15680</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>XMP + L-glutamine + ATP + H2O = GMP + L-glutamate + AMP + diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="10"/>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877397.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT94"/>
    <dbReference type="GeneID" id="76208419"/>
    <dbReference type="OrthoDB" id="33844at2157"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003921">
        <property type="term" value="F:GMP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01742">
        <property type="entry name" value="GATase1_GMP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01997">
        <property type="entry name" value="GMP_synthase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000002">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000001">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000001">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00344">
        <property type="entry name" value="GMP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001674">
        <property type="entry name" value="GMP_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004739">
        <property type="entry name" value="GMP_synth_GATase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022955">
        <property type="entry name" value="GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025777">
        <property type="entry name" value="GMPS_ATP_PPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00884">
        <property type="entry name" value="guaA_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00888">
        <property type="entry name" value="guaA_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000848">
        <property type="entry name" value="PRK00074.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922:SF2">
        <property type="entry name" value="GMP SYNTHASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922">
        <property type="entry name" value="GMP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00958">
        <property type="entry name" value="GMP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51553">
        <property type="entry name" value="GMPS_ATP_PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5 10 11" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMPS ATP-PPase" evidence="12">
        <location>
            <begin position="193"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="218"/>
            <end position="224"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH34777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="507" mass="56336" checksum="A1C2E3A71B34654F" modified="2018-07-18" version="1">MDKIVVLDFGSQYSHLICRRIREFSVYAELVPYDISFEELQKLNPKGIIFSGGPSSVYNSDAPVPENKIFEMNLPLLGICYGHQLIVNKYGGKVKRANKEYGSSLLTIDNDKDLLNGIGESVRAWMSHGDEAEQIPPGFQVIGHTEGAKAAAIASENKSIYGIQFHPEVVHTEQGTEILKNFVLKVCGANQDWTMEGFIDSAVEKISKIEGNVLCGVSGGIDSTVVALLIHKAIGDRLKCVFVNNGLLRLNEEKEIEAMFKDNFKVNFTSIDAADQFLGKLKGVEDPEKKRMIVGEEFIHVFTEFAEKNGPFKWLAQGTLYPDVIESGVSKGPAAVIKSHHNVGGLPDWLNLEILEPLRELYKDEVRKIAKILQVPEKLFMRHPFPGPGLAVRIIGEVTPTKLTIAKVASRIVEDELLEANLYDKVWQAYAAVGDDRAVGVVGDERRYGNIVMIRVVDSIDAMTADWTRLPHGLLEKMSNRITNEIEDVTWVTYTISSKPPATIEPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTI9</accession>
    <name>A0A2S2KTI9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cobalt-precorrin-7 (C(5))-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_17810</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877586.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTI9"/>
    <dbReference type="GeneID" id="76210340"/>
    <dbReference type="OrthoDB" id="42238at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11644">
        <property type="entry name" value="Precorrin-6Y-MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012818">
        <property type="entry name" value="CbiE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051810">
        <property type="entry name" value="Precorrin_MeTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02467">
        <property type="entry name" value="CbiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47036">
        <property type="entry name" value="COBALT-FACTOR III C(17)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47036:SF1">
        <property type="entry name" value="COBALT-FACTOR III C(17)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="238" mass="27023" checksum="120F226AFA1BFDB1" modified="2018-07-18" version="1">MGKIFAVGVGPGSPKYVTEIVKEIIENCDVIIGYKYTLKTIEHLTEGKEVYEITMNNQEESYQKILPELGDKTLVIPFTGDVNFSESEVVDRLIEIFGKVEIIPGISSIQVAASKAQVPLDKSKIITMHVTTPIDDKKLELQKALIDGFSVILVPRPWPKQPDKHFMPSEIAVYLRVNNFDTSKIKVHVYEAITTENETSFVGTVKDLEGKEFSDLSVMVFNQTRLDSYMNYKWQWKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTJ9</accession>
    <name>A0A2S2KTJ9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_17630</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877569.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTJ9"/>
    <dbReference type="GeneID" id="76208222"/>
    <dbReference type="OrthoDB" id="7442at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000047">
        <property type="entry name" value="NADP-dependent L-serine/L-allo-threonine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43115">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE SDR FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43115:SF4">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="246" mass="26709" checksum="51709885A2C23890" modified="2018-07-18" version="1">MIKDKVAIITGASSGIGFATALALSKAGAKVAIGARRVDRLEELAKKISTDGGEVFFQKLDVTQRIECENFANAVLEKWGSIDILVNNAGLMPLSFFKNLKIDEWDRMIDVNIKGVLYSTASVISHMKEKKSGHIVNLSSVAGRIVFPAGSVYCATKHAVAAFSEGLRQEFSVRTNIRVTSIEPGVVATELNDTITDESLQGFVQNAKKMEALQAEDIANAILYAVDSPSHVNVNEILIRPTTQER</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTK6</accession>
    <name>A0A2S2KTK6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nicotinamide-nucleotide adenylyltransferase</fullName>
            <ecNumber evidence="4">2.7.7.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">NAD(+) diphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">NAD(+) pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">NMN adenylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_17020</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>beta-nicotinamide D-ribonucleotide + ATP + H(+) = diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21360"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="EC" id="2.7.7.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the archaeal NMN adenylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.1" evidence="4"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTK6"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00600"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00243">
        <property type="entry name" value="NMN_adenylyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006418">
        <property type="entry name" value="NMN_Atrans_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342:SF0">
        <property type="entry name" value="BIFUNCTIONAL NMN ADENYLYLTRANSFERASE_NUDIX HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342">
        <property type="entry name" value="PHOSPHOPANTETHEINE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 4 6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="5">
        <location>
            <begin position="2"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="151" mass="17504" checksum="8BB6D1D93B8CA51D" modified="2018-07-18" version="1">MGHLEALRFALSKVDRLWIGLGSSNKPTEKNNPFSAEERREMILSSINDSMKKRISIYFIPDLDNHVKWIEKIDTIVPKFDIVFSNDELTNHLYSKRTVKVVPIPFLKRNELSGTNIRNLIISDQKWDHLVPEGTKNFLINTSANDRLKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTM3</accession>
    <name>A0A2S2KTM3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_18110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877612.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTM3"/>
    <dbReference type="GeneID" id="76210310"/>
    <dbReference type="OrthoDB" id="11910at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="184"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="344" mass="39162" checksum="2719B64C89BE3844" modified="2018-07-18" version="1">MNTIVTFGLLILVASMALGGITNSIYAQDDPSILLKLAKRAQNQIQNQISSESPDEIKRLFEEGIQNVNYLEKAIRNNDTVSAKEHFLSAMKIFTEISRHLSTSDDAAAKSSRDISEETPTNPARSTAKDPSNDIQRLQVYVNNLKSLAEKYETSIDFSELDKLFELSRQQISDNQFSLASETLQKIQEIVVEINKEIREEAAKQESQRAKEYAQQYLEQLDRLIENAKKQGLSDEIINKLENARENLSSADNPADIVQEIRKILSIKKQFELTKNDRLESRVLQIEKTISQLSQIDGVDHDILVDAKETLQTIKRYLYDGEFDNANQLLRDLAKQLEEIKNSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTQ2</accession>
    <name>A0A2S2KTQ2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GTP cyclohydrolase 1</fullName>
            <ecNumber evidence="5">3.5.4.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">GTP cyclohydrolase I</fullName>
            <shortName evidence="5">GTP-CH-I</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">folE</name>
        <name evidence="7" type="ORF">NZNM25_18140</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>GTP + H2O = 7,8-dihydroneopterin 3'-triphosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17473"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58462"/>
            <dbReference type="EC" id="3.5.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homopolymer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the GTP cyclohydrolase I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.16" evidence="5"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877615.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTQ2"/>
    <dbReference type="GeneID" id="76210307"/>
    <dbReference type="OrthoDB" id="8438at2157"/>
    <dbReference type="UniPathway" id="UPA00848">
        <property type="reaction ID" value="UER00151"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003934">
        <property type="term" value="F:GTP cyclohydrolase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.286.10:FF:000001">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1130.10:FF:000001">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00223">
        <property type="entry name" value="FolE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043134">
        <property type="entry name" value="GTP-CH-I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001474">
        <property type="entry name" value="GTP_CycHdrlase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018234">
        <property type="entry name" value="GTP_CycHdrlase_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020602">
        <property type="entry name" value="GTP_CycHdrlase_I_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00063">
        <property type="entry name" value="folE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006825">
        <property type="entry name" value="PRK09347.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006826">
        <property type="entry name" value="PRK09347.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109:SF7">
        <property type="entry name" value="GTP CYCLOHYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109">
        <property type="entry name" value="GTP CYCLOHYDROLASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01227">
        <property type="entry name" value="GTP_cyclohydroI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00859">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00860">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 5" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="GTP cyclohydrolase I" evidence="6">
        <location>
            <begin position="7"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH35023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="185" mass="21311" checksum="D5F78C3FA7B9A677" modified="2018-07-18" version="1">MDKERVKKLVRELIIEIGEDPTREGLRETPDRIASMYKEIFEGYDSDSELSVQFSEDSDVVIARDIQFYSMCEHHMLPFFGKIHIAYSPNGRVFGISKLVRLVEKYSKRLQIQERLTKNIADELYSQGVKGVVVLADAEHLCMKMRGVRNDATLSSSAFRGIYENKEEKAGIMTLIRKRTSYSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTR1</accession>
    <name>A0A2S2KTR1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_18230</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877622.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTR1"/>
    <dbReference type="GeneID" id="76210298"/>
    <dbReference type="OrthoDB" id="9973at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="103" mass="12676" checksum="39D6F4836FAD8470" modified="2018-07-18" version="1">MPNCPECTSREKKKIEAKYEEEIPEEERSRDALFKLFDEIDIPMKMDEKNRRHFICKRCGLYATREEISDIRFKLNQRERTRDDKHDDYLEWWSKSKKEKAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTT1</accession>
    <name>A0A2S2KTT1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutamate racemase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">murI</name>
        <name evidence="2" type="ORF">NZNM25_18430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877642.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTT1"/>
    <dbReference type="GeneID" id="76210278"/>
    <dbReference type="OrthoDB" id="6363at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016855">
        <property type="term" value="F:racemase and epimerase activity, acting on amino acids and derivatives"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1860">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001920">
        <property type="entry name" value="Asp/Glu_race"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21198">
        <property type="entry name" value="GLUTAMATE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21198:SF3">
        <property type="entry name" value="GLUTAMATE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53681">
        <property type="entry name" value="Aspartate/glutamate racemase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="254" mass="28412" checksum="AB284F8FE2A9BD94" modified="2018-07-18" version="1">MVKIAVFDSGLGSLSIIKPIQKQMKCDIIYFADTKNFPYGKKPIKELKSITLKTISSIQNSFAPKLIVIGSNTLSLTLDSHSNNIFTVLPPINEAKAISKSKSIAILATESIVKSKLLDNYIKSLKINNIKIIKINASALVDLVESGKFYSNPELCKNMIKKILSLTFLKNNVDVVTLSSTHLPFLLKFFKKIFPNIVFLDPSESLAINLKQHYADPNKKRNSLQIFTSGSIKLLEKKLSYMNINNKISKFSIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTV9</accession>
    <name>A0A2S2KTV9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_17880</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048114480.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTV9"/>
    <dbReference type="GeneID" id="24819314"/>
    <dbReference type="OrthoDB" id="109at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="72" mass="8533" checksum="FBEECFFAA942EA0C" modified="2018-07-18" version="1">MKLYLDFNPCQECNTMMAELSSPEMLFADEKTRADESAKFLRHLTYNHNEVVQAVMEDLPKQKKDQEPDFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTW2</accession>
    <name>A0A2S2KTW2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_19010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTW2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052048">
        <property type="entry name" value="ST_Response_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228:SF1">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ARR22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="1">
        <location>
            <begin position="7"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="125" mass="14213" checksum="28D0BD3BCC8542AD" modified="2018-07-18" version="1">MDMINYKVLIADDSAFMRTLLSKIFQHTTGVGEIVQVNNGEEALKAYKVQKPDLVTMDIDMPEMDGIEAAKLIKQYDSKAKIVIVSSSDKKNTRDEAEKIGVFGYIRKPFDRLEIKRIIDDLSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTY3</accession>
    <name>A0A2S2KTY3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Acyl-CoA synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">NZNM25_18770</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877700.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTY3"/>
    <dbReference type="GeneID" id="76210104"/>
    <dbReference type="OrthoDB" id="18103at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043758">
        <property type="term" value="F:acetate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000020">
        <property type="entry name" value="Protein lysine acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053580">
        <property type="entry name" value="4-HB-CoA_ligase_ADP-forming"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051538">
        <property type="entry name" value="Acyl-CoA_Synth/Transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043938">
        <property type="entry name" value="Ligase_CoA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032875">
        <property type="entry name" value="Succ_CoA_lig_flav_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045493">
        <property type="entry name" value="HydDutCoALig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43334">
        <property type="entry name" value="ACETATE--COA LIGASE [ADP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43334:SF2">
        <property type="entry name" value="ACETATE--COA LIGASE [ADP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13549">
        <property type="entry name" value="ATP-grasp_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19045">
        <property type="entry name" value="Ligase_CoA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13607">
        <property type="entry name" value="Succ_CoA_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="10">
        <location>
            <begin position="491"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH35086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="698" mass="75963" checksum="714FB9EE4939EBCE" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTZ6</accession>
    <name>A0A2S2KTZ6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_19310</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877743.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTZ6"/>
    <dbReference type="GeneID" id="76210161"/>
    <dbReference type="OrthoDB" id="57329at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056798">
        <property type="entry name" value="ADH_Fe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039697">
        <property type="entry name" value="Alcohol_dehydrogenase_Fe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496:SF83">
        <property type="entry name" value="HYDROXYACID-OXOACID TRANSHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25137">
        <property type="entry name" value="ADH_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA" evidence="2">
        <location>
            <begin position="7"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Fe-containing alcohol dehydrogenase-like C-terminal" evidence="3">
        <location>
            <begin position="156"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH35140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="314" mass="34870" checksum="AFCB06B9FD1CB933" modified="2018-07-18" version="1">MYTIRQPKKIIFGKNSCCQFSYPENTLVITSSGAISRGWIDYLKIKNYEVFDSVESNPSMNTVTKILSKFTHNYSSIIGIGGGSSLDVAKYVAFKLKKPKILIPTTFGSGSEVTRISVLKIDGKKISFHADELLADVAIVDPFFLNSASDKVIKNSAIDACAQCSEAYDSKLANTFTKFLCLKAFNILENAILSNNLSNLAYGSMLSGLGFGNSSTTLGHALSYVFSNEGYSHGHALAFTTTIAHQFNNSQFYKRFKDLVFKLDFEPIQLTQNLSEAANLIQFDRKHLDYNPKIVSNYDIVKMLQTINSQKIFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU11</accession>
    <name>A0A2S2KU11_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MIP18 family-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_19240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877737.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU11"/>
    <dbReference type="GeneID" id="76210154"/>
    <dbReference type="OrthoDB" id="371709at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1150">
        <property type="entry name" value="Coenzyme PQQ synthesis protein D (PqqD)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052339">
        <property type="entry name" value="Fe-S_Maturation_MIP18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008792">
        <property type="entry name" value="PQQD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041881">
        <property type="entry name" value="PqqD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42831">
        <property type="entry name" value="FE-S PROTEIN MATURATION AUXILIARY FACTOR YITW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42831:SF1">
        <property type="entry name" value="FE-S PROTEIN MATURATION AUXILIARY FACTOR YITW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05402">
        <property type="entry name" value="PqqD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIP18 family-like" evidence="1">
        <location>
            <begin position="7"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="212" mass="23535" checksum="8E020B7186F5C5FC" modified="2018-07-18" version="1">MSTVSPQAIEESLKQCMDPEVPLNIVEMGLIYGIDVAENNDVNIKMTMTTQGCPLHETLVQDATRYAKKVPGVNNVKIDIVWEPAWSMDKMTDEGKMKLKNMGAAMNTPAPINYETALPQGVGKLVQQEDGSMVLANEHDQGFMVNQAIVDFWKSCNGQRKVTELVEVFAQQTGLQRNQVEKEVMQLLQQLREGGLIAIAGQPDTPNVEFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU16</accession>
    <name>A0A2S2KU16_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sugar nucleotidyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_19350</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877747.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU16"/>
    <dbReference type="GeneID" id="76210165"/>
    <dbReference type="OrthoDB" id="25155at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02523">
        <property type="entry name" value="PC_cytidylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050065">
        <property type="entry name" value="GlmU-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43584:SF8">
        <property type="entry name" value="N-ACETYLMURAMATE ALPHA-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43584">
        <property type="entry name" value="NUCLEOTIDYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MobA-like NTP transferase" evidence="3">
        <location>
            <begin position="3"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH35144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="249" mass="28546" checksum="C0DDD51D24729774" modified="2018-07-18" version="1">MNAIIIAAGSGKRISNEVKDIPKSMVKVNEKSIIEHQISVLKQAEIDEIYVITGPHSEKFDLNHVKYIKDQNYTEHDILGSLMEAKNFLKNDILVLYSDIIFDLPIIQQMLDSKDDISIAIDMNWEKMYEGRTLHPKSEAENVELNYAKKIIKIQKNIQNNNNCIGEFLGIVKFSPTGSKLFVKKYEELAKTHIGNFQQADSISKAYLTDMIQELIDSNIHVEPIFISGKWCEIDTIQDLRNAEKIFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU22</accession>
    <name>A0A2S2KU22_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_19200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877733.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU22"/>
    <dbReference type="GeneID" id="76210150"/>
    <dbReference type="OrthoDB" id="11967at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="162" mass="18375" checksum="D8B5FFCEE2B42E6D" modified="2018-07-18" version="1">MKLSLFKLGLSLVIIGMVWCSFIFAESEKTSDSIILKQSNSFELKSEFMGEDIGFYKLFMPEFSGEEIFVQILDSQDNVIQEERIQTKMSVGYFDFEDGTFTIKVTNISKTPVDLQIEFGDTNSHEMVPAGILVLAGAVAMIIISYFKIQNYKIEHPEENIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU26</accession>
    <name>A0A2S2KU26_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycosyltransferase RgtA/B/C/D-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_19610</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877771.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU26"/>
    <dbReference type="GeneID" id="76210191"/>
    <dbReference type="OrthoDB" id="3370at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050297">
        <property type="entry name" value="LipidA_mod_glycosyltrf_83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038731">
        <property type="entry name" value="RgtA/B/C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33908">
        <property type="entry name" value="MANNOSYLTRANSFERASE YKCB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33908:SF11">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13231">
        <property type="entry name" value="PMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="130"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="295"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="319"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase RgtA/B/C/D-like" evidence="9">
        <location>
            <begin position="91"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH35170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="508" mass="59469" checksum="3C13D75C0EA1853F" modified="2018-07-18" version="1">MMSLKLIKNDIKISFLTIFLAGLGIKLFYFPYDLPLIIDGMDNFTYATAINFYGHLPTEWTPINNGWPIFVSFWFSIFDLENSLQYMQLQRIISVILSSMITIPVYYLCKNYFNKKIALVGAAIISFDPRIILNSFLGITEPLFILLGISSLVIFLKYERKLMFISFILASFCTIVRSEGLFLFFTLTILFFIKNKISKEILRTYLPCLLIFMMILIPVINYRIEVVGYDGIFQRAIIGTDQIIAISNSDKNSEIIDGIGLFIKYLGWIMIPNFLLFFPFGVIQYFRNRTKETNFIMIFSIVCSIPIMYAYIVQAQDTRYLYFLFPIFSLVSLYSVKTFISKIANKNTALVVIIIGIFISSIGFYEFLKTDREIERENYEISKIISEKVSGVNFHPSQTQYIRVGELPTKWPFDFNDDKHKIKIISTNNENDLEEYILKNDSELTHLIVDENSKLPKFLQDVYYNEEKFKYLDKVFDSKDFNFNQHLKLFEINFENFNSKINQEELSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU32</accession>
    <name>A0A2S2KU32_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Heat-shock protein Hsp20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_18580</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877682.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU32"/>
    <dbReference type="GeneID" id="76210085"/>
    <dbReference type="OrthoDB" id="198277at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="4">
        <location>
            <begin position="48"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="49"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH35067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="162" mass="18514" checksum="D8AC7212EC3D872A" modified="2018-07-18" version="1">MVSKNTPKKSSRGISPWHSSWEELDKTFENFRRDLEKSFSSFPSISMPRFPHMQETSCDVIDEGKQLRVKMNVPGIQKKDIKLNVTDNSLEVSAEHKEESEEKKKNYLRKERSQVSYYRTLPLSEKVVSGQAKAKLTDGVLDIVLPKSTPTKVQAKKSVSVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU36</accession>
    <name>A0A2S2KU36_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_17760</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877581.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU36"/>
    <dbReference type="GeneID" id="76208209"/>
    <dbReference type="OrthoDB" id="5891at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="133" mass="15698" checksum="E02EF0F8D6392190" modified="2018-07-18" version="1">MTNKRIRIGMEDSDGAKYDIKLEGNVTREKVLKIFEMMDLMNIEEQSETTNMDSMGSKIWHIIDKFFPMGKFTSTNILEKYEDEFNEPIKLSIISTYLSRFSAKGRVNRTRTGREWIYQTIKIGQKHELFSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU47</accession>
    <name>A0A2S2KU47_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Heat-shock protein Hsp20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_17860</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877591.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU47"/>
    <dbReference type="GeneID" id="76210335"/>
    <dbReference type="OrthoDB" id="26084at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041800">
        <property type="entry name" value="Hsp20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="67"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="166" mass="18906" checksum="6C344419247A9060" modified="2018-07-18" version="1">MTMFFDSEFDRIFKRMSNSFFNIDDIFEEFKGNGPTTGPYYYGYTMTIGPDGKPVVKEYGNVKPGLLPTSDTREPVVDTIIDEKEKVVKLIAEMPGVEKTDVKVVVENKIVELFAEHDKKKYHAKVPVQQKIDENSAKASYKNGVLEVIFKLIEDEKPKGKTVEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU65</accession>
    <name>A0A2S2KU65_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Snf7 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_18060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU65"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="24590" checksum="DB263B86750E279C" modified="2018-07-18" version="1">MRLSTYSIIEIMPNLTKDWSKQPQPSISEKINDTIKPKGALKPRVQEGIKKLQNQIRKLDSMLTNLQERDAKLFQRIVDATQKHDTQTSKVLGNELAEVRKVTKILSSARIALEQIELRLTTCSDLGDTVVAIMPTMGLMKNLKSSLGKIMPGAEQEIGQMAEMLGGFMTESFSGDAAFGMDATTNAESESILKEAAAVAESSTGQMFPSVPTNTKEATSTKFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU84</accession>
    <name>A0A2S2KU84_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_19670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877777.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU84"/>
    <dbReference type="GeneID" id="76210197"/>
    <dbReference type="OrthoDB" id="8570at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="129" mass="15579" checksum="B24BEE585F6D3FEE" modified="2018-07-18" version="1">MKEGWARRRWWEFRQGHSVYLIFVLTFINFILIAYRLLIERVIFFKELIPELWIFAVMFIIFYIPAATLIGYWHRKTQLRVETTLVQQQNPVLARMIRTLLDVQTGKASEEEIAEFRKMLNNIEKRLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUB0</accession>
    <name>A0A2S2KUB0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Methionine adenosyltransferase</fullName>
            <ecNumber evidence="5 13">2.5.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">metK</name>
        <name evidence="17" type="ORF">NZNM25_20100</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the AdoMet synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="5 13"/>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877818.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUB0"/>
    <dbReference type="GeneID" id="76210244"/>
    <dbReference type="OrthoDB" id="6056at2157"/>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18079">
        <property type="entry name" value="S-AdoMet_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000003">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000004">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00086">
        <property type="entry name" value="S_AdoMet_synth1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022631">
        <property type="entry name" value="ADOMET_SYNTHASE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022630">
        <property type="entry name" value="S-AdoMet_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022629">
        <property type="entry name" value="S-AdoMet_synt_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022628">
        <property type="entry name" value="S-AdoMet_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002133">
        <property type="entry name" value="S-AdoMet_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022636">
        <property type="entry name" value="S-AdoMet_synthetase_sfam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01034">
        <property type="entry name" value="metK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11964">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02773">
        <property type="entry name" value="S-AdoMet_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02772">
        <property type="entry name" value="S-AdoMet_synt_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00438">
        <property type="entry name" value="S-AdoMet_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000497">
        <property type="entry name" value="MAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55973">
        <property type="entry name" value="S-adenosylmethionine synthetase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00376">
        <property type="entry name" value="ADOMET_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00377">
        <property type="entry name" value="ADOMET_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="12" id="KW-0630">Potassium</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S-adenosylmethionine synthetase N-terminal" evidence="14">
        <location>
            <begin position="5"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase central" evidence="15">
        <location>
            <begin position="118"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase C-terminal" evidence="16">
        <location>
            <begin position="236"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="NCBIfam" id="TIGR01034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GBH35219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="388" mass="43066" checksum="05E04BB34C5C1E07" modified="2018-07-18" version="1">MTNSFLFTSESVTEGHPDKICDNISDAFLDEFLKQDPDSRVAVETMVTTDFVAVAGEVTSKANFDKKAQEELVRKTIRDIGYNNKDLMFDTETCEVTLRLHSQSPDISQGVTATKEKEQGAGDQGLMFGYATNETKELMPMPILLSQKLAQKLAEVRKNNILPWARPDGKTQVSVRYEDNKPIRIETVVVSTQHAPEISQEEIAKEVIDKVIKPVLGNLWNDEIKIHINPTGKFVIGGPHGDAGLTGRKIIVDTYGGFGRHGGGAFSGKDPSKVDRSACYMCRYIAKNLVAAELADRCEVQLAYAIGVAEPVSLYVNTFGTNKIPENEIEKLVRKNFDMKPSGIISQLDLKRPIYKKTASYGHFGRNEPEFTWEKTDKAETLKQSAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUE1</accession>
    <name>A0A2S2KUE1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamine amidotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_20270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877846.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUE1"/>
    <dbReference type="GeneID" id="76210262"/>
    <dbReference type="OrthoDB" id="10772at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044992">
        <property type="entry name" value="ChyE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695:SF5">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="180" mass="20271" checksum="EE6CFC97FEBAF7C1" modified="2018-07-18" version="1">MLLVVDNGSVYTKNLTDFLLNKNIPFEKQTPQSLDLNSLGNYDCFILSGRRKNEKKTNEINSKIVNYSLKNNVKLLGICYGAEILALTLGGTIRKSSSLQKGNEKIKILTDNPICSDSLDVFESHGFEISKLPDVFVSLGESNYCKYEIIQYKKKPIFGTQFHPEMTKDGNSLIEKFCSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUE3</accession>
    <name>A0A2S2KUE3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_19680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877778.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUE3"/>
    <dbReference type="GeneID" id="76210198"/>
    <dbReference type="OrthoDB" id="378364at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="133" mass="15836" checksum="A16B72070DFFA8EF" modified="2018-07-18" version="1">MNLKKNFLRKLWWDGRMGHSNYLMFFLAFVNFILITYSFLIEGNEIFEQYISDLGLFTVIFLIFYFPVSILIGRWHTKTQISVEMTMKMNEDPIMAKMIRTLLDVQTGKASEEEIAEFRKIVAEIEKQDINEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUR3</accession>
    <name>A0A2S2KUR3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphatidylinositol glycan-class A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_19790</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877788.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUR3"/>
    <dbReference type="GeneID" id="76210209"/>
    <dbReference type="OrthoDB" id="132546at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050194">
        <property type="entry name" value="Glycosyltransferase_grp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947:SF3">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="2">
        <location>
            <begin position="13"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="1">
        <location>
            <begin position="167"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="340" mass="37689" checksum="3FC3124E73A3974A" modified="2018-07-18" version="1">MKILIISPTLKGIGGVAQHVRDLIKFLKENGHCVDVLSSENTPIIPIKKLRNPSFLISSFFKAKLMKNYDIVHAQHPIGALAMKNVSGKKILTIHGIYSQQIGMLHGKTSSTFSTKYERNAFEWADAVTAGSKEAFEYYSKLGSKVSFIPNAIDISSLPPGIDTRYEKQIIFAGRLSKEKGILTILKMADELPDDINLLIIGNGPEKESVVEVTKNHANIHYLGYQPKEKTIPLIRGSKLLIQPSLAEGISATLLEAMACQTPVIATNIGGNVELFENNKTGILIEPENPAKLLREIVSILNNPIKLEQISESAFKQVQKYDWPNIGKKYLSLYEKLLHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNP0</accession>
    <name>A0A2S2KNP0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoribosylformylglycinamidine synthase subunit PurL</fullName>
            <shortName evidence="8">FGAM synthase</shortName>
            <ecNumber evidence="8">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Formylglycinamide ribonucleotide amidotransferase subunit II</fullName>
            <shortName evidence="8">FGAR amidotransferase II</shortName>
            <shortName evidence="8">FGAR-AT II</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Glutamine amidotransferase PurL</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Phosphoribosylformylglycinamidine synthase subunit II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">purL</name>
        <name evidence="12" type="ORF">NZNM25_00510</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the FGAMS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="8"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875931.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNP0"/>
    <dbReference type="GeneID" id="76209380"/>
    <dbReference type="OrthoDB" id="8251at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02203">
        <property type="entry name" value="PurL_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02204">
        <property type="entry name" value="PurL_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00420">
        <property type="entry name" value="PurL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010074">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041609">
        <property type="entry name" value="PurL_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01736">
        <property type="entry name" value="FGAM_synth_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002290">
        <property type="entry name" value="PRK01213.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43555">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43555:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18072">
        <property type="entry name" value="FGAR-AT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001587">
        <property type="entry name" value="FGAM_synthase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribosylformylglycinamidine synthase linker" evidence="11">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like N-terminal" evidence="9">
        <location>
            <begin position="60"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="10">
        <location>
            <begin position="186"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like N-terminal" evidence="9">
        <location>
            <begin position="418"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="10">
        <location>
            <begin position="548"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="34"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="79"/>
            <end position="82"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="294"/>
            <end position="296"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="474"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="511"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="514"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH33260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="721" mass="78597" checksum="AF2A0C844D6A318D" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNS7</accession>
    <name>A0A2S2KNS7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Superoxide dismutase</fullName>
            <ecNumber evidence="2 6">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">sodA_1</name>
        <name evidence="9" type="ORF">NZNM25_01110</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the iron/manganese superoxide dismutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="2 6"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875985.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNS7"/>
    <dbReference type="GeneID" id="76209440"/>
    <dbReference type="OrthoDB" id="32917at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.55.40.20:FF:000004">
        <property type="entry name" value="Superoxide dismutase [Fe]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.990">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="entry name" value="Iron/manganese superoxide dismutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001189">
        <property type="entry name" value="Mn/Fe_SOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019833">
        <property type="entry name" value="Mn/Fe_SOD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019831">
        <property type="entry name" value="Mn/Fe_SOD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S26, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00081">
        <property type="entry name" value="Sod_Fe_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000349">
        <property type="entry name" value="SODismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01703">
        <property type="entry name" value="MNSODISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00088">
        <property type="entry name" value="SOD_MN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Manganese/iron superoxide dismutase N-terminal" evidence="7">
        <location>
            <begin position="3"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="8">
        <location>
            <begin position="99"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000349-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="207" mass="24167" checksum="C7A8EA6917C4D535" modified="2018-07-18" version="1">MVRYELPRLPYGYDELEPFIDTETMKIHHQKHHQSYVDGLNKSLEEIGGASHPKYISSILSELTSIPESGRSTINFFGGGFENHRLFWETMTPNGEGKPNGKLDDAIDVYFDSFENFKKIFSEKAIAIQGSGWCWLVFNQTYNKIEIITTQNQDSPWTVQKTPLLGLDVWEHAYYLKYQNKRPDYVQAWWNVVNWDYVGNRFSELPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNU3</accession>
    <name>A0A2S2KNU3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01340</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876005.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNU3"/>
    <dbReference type="GeneID" id="76209463"/>
    <dbReference type="OrthoDB" id="331at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="61" mass="6760" checksum="8E08E6237731AD3B" modified="2018-07-18" version="1">MTSETESKIKETGISTAELISHFREKCKLCGHHRIMHNEFGACEGVMNKPCTSGCDGFDPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNW9</accession>
    <name>A0A2S2KNW9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Flavin prenyltransferase UbiX</fullName>
            <ecNumber evidence="6">2.5.1.129</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ubiX</name>
        <name evidence="8" type="ORF">NZNM25_00580</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Flavin prenyltransferase that catalyzes the synthesis of the prenylated FMN cofactor (prenyl-FMN) for 4-hydroxy-3-polyprenylbenzoic acid decarboxylase UbiD. The prenyltransferase is metal-independent and links a dimethylallyl moiety from dimethylallyl monophosphate (DMAP) to the flavin N5 and C6 atoms of FMN.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>dimethylallyl phosphate + FMNH2 = prenylated FMNH2 + phosphate</text>
            <dbReference type="Rhea" id="RHEA:37743"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:87467"/>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
            <dbReference type="EC" id="2.5.1.129"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the UbiX/PAD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.129" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876247.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNW9"/>
    <dbReference type="GeneID" id="76209387"/>
    <dbReference type="OrthoDB" id="9540at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106141">
        <property type="term" value="F:flavin prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1950:FF:000001">
        <property type="entry name" value="Flavin prenyltransferase UbiX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01984">
        <property type="entry name" value="ubiX_pad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004507">
        <property type="entry name" value="UbiX-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004685">
        <property type="entry name" value="PRK06029.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00421">
        <property type="entry name" value="ubiX_pad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43374">
        <property type="entry name" value="FLAVIN PRENYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43374:SF1">
        <property type="entry name" value="FLAVIN PRENYLTRANSFERASE PAD1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 6" id="KW-0288">FMN</keyword>
    <keyword evidence="1 6" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Flavoprotein" evidence="7">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="9"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="86"/>
            <end position="89"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>dimethylallyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>dimethylallyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="185" mass="20451" checksum="FE8BFBDBF71CA701" modified="2018-07-18" version="1">MKLVIGITGSTGVIYGIRMLETLKKLNIETHLIMSEWAQKCISMETDYSLEYVTSLATSISDEKNMASSVSSGTHRIDGMIVAPCSMKTLAAIANGYDDTLIARAAGVTIKESRKLILMVRETPLSAIHLENMLKLSRIGVVILPPVTEFYTKPKSIDDIVNHGVGKCLDQFDVEHDLYPRWGTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNX2</accession>
    <name>A0A2S2KNX2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_00020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875888.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNX2"/>
    <dbReference type="GeneID" id="76209327"/>
    <dbReference type="OrthoDB" id="9652at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00156">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051271">
        <property type="entry name" value="2C-system_Tx_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040572">
        <property type="entry name" value="TackOD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45526:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN DCUR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45526">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN DPIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18551">
        <property type="entry name" value="TackOD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="1">
        <location>
            <begin position="6"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="308" mass="34997" checksum="D6B8D3D161195E27" modified="2018-07-18" version="1">MGAKDVVLIIDDSTAIGILLTEFLKKLGYADIKTTATGESGLALFKELINNKIIPLVFLDYNLPDTNAKSVMSQILIIQPNTKIIIETASGKDEDPIKEVIGLGAYHYIQKPIHFEAIKNVMKILEEEESILEHTSSVDNDGCHLIDRYFNTYKRVTLSRLIEQSNLSEKDVTTYLQKLISEGKVVALQNIREICCNACGSLKLAQIYQCPNCKNSNFDQVKLIEHFDCGNFSPESTYDNDKCPKCKKQIKALGVDYRVLHNRYLCKKCNEIFQDISNESLCLKCNTSFKIDSGHWKESMEYKLMHDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNX8</accession>
    <name>A0A2S2KNX8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876008.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNX8"/>
    <dbReference type="GeneID" id="76209466"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="104" mass="11107" checksum="33A2D0792B0ABB25" modified="2018-07-18" version="1">MTTITVVFAILAVALTTTIGITPAFADFQDYIWSRSAGDGHLGFLNCSGDNCDLKLKTTSGVQIHSQSTINSEVDSVVTEFDSVGKKMSIDRVTTADSIFVNPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNZ4</accession>
    <name>A0A2S2KNZ4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SF3 helicase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_01570</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNZ4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006500">
        <property type="entry name" value="Helicase_put_C_phage/plasmid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014015">
        <property type="entry name" value="Helicase_SF3_DNA-vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045455">
        <property type="entry name" value="NrS-1_pol-like_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051620">
        <property type="entry name" value="ORF904-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014818">
        <property type="entry name" value="Phage/plasmid_primase_P4_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01613">
        <property type="entry name" value="primase_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35372">
        <property type="entry name" value="ATP BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35372:SF2">
        <property type="entry name" value="SF3 HELICASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08706">
        <property type="entry name" value="D5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19263">
        <property type="entry name" value="DUF5906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00885">
        <property type="entry name" value="D5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51206">
        <property type="entry name" value="SF3_HELICASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SF3 helicase" evidence="4">
        <location>
            <begin position="163"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="453" mass="53258" checksum="A532151F1FDD6761" modified="2018-07-18" version="1">MEIFAVENNLKFEGEEKIDQVVKLLRKFYNFVTLRKTEEILLYNGKIYDNIQAETVIKEKTEKLIPNCTTHNRHEVINKIKAQTFVDLEKFDADPNLITVENGILNLQTLKLEPHTPNHLSRVLLPVEFHKPKSTNIEKNLKNTLFWKYLKSSFTVKRRFRIEDFETVLEITASPIIKRHIDEKAFMFLGNGENGKSVLLFYIKSYLGENNVSSISLHDIAENKFMRANLAGMSANIFPDLEENELRNAGKIKAIISNEGIEVEKKYQQGFTLYPFAKLMFSCNRFPKVFDQSQGFFRRWIIVKWERDFENDPERIEYLKESLAENQEEKNKVFSNLVLIAHKLNKVGKFTHTKNWKTIQREWSENADPIDEFNSSYILDSESHKTKKETYHFYKEIMLEKGQTPKGMGQFSKAFSEWHDEDRIEIDGRVQRVWLNISFKMPKQETLATVDTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNZ6</accession>
    <name>A0A2S2KNZ6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Small ribosomal subunit protein eS4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rps4e</name>
        <name evidence="11" type="ORF">NZNM25_00320</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the eukaryotic ribosomal protein eS4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875914.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNZ6"/>
    <dbReference type="GeneID" id="76209358"/>
    <dbReference type="OrthoDB" id="372073at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00485">
        <property type="entry name" value="Ribosomal_eS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000876">
        <property type="entry name" value="Ribosomal_eS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013845">
        <property type="entry name" value="Ribosomal_eS4_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038237">
        <property type="entry name" value="Ribosomal_eS4_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013843">
        <property type="entry name" value="Ribosomal_eS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003312">
        <property type="entry name" value="PRK04313.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00900">
        <property type="entry name" value="Ribosomal_S4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08071">
        <property type="entry name" value="RS4NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 11" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 7 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein eS4 N-terminal" evidence="10">
        <location>
            <begin position="6"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="Small ribosomal subunit protein eS4 central region" evidence="9">
        <location>
            <begin position="98"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH33241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="238" mass="26479" checksum="DC0DDDF513BDF813" modified="2018-07-18" version="1">MVSISGSKKLKRQMAPQFWGIARKDKRFVINVKPGPHKRNHAVPTAVFLRDMLKIVTSLREAKASIYSGKVKVDGIVRKSLHHAIGLMDVVELENVSDIYRLVPTEEKLLKPIKINESEKSKKLVRVTTKTTIGKGQTQIGFHDGRSIISDSKVNVGDTCLIQIPDVKILEIIKLEVGSHGLVTRGNNTGRIGRIETIEEGTFILPKRVILSLDERKIEIPADIIMSIGKGEPVIQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP00</accession>
    <name>A0A2S2KP00_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Proteasome subunit beta</fullName>
            <ecNumber evidence="9">3.4.25.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">20S proteasome beta subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Proteasome core protein PsmB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">psmB</name>
        <name evidence="10" type="ORF">NZNM25_01670</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.25.1" evidence="9"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP00"/>
    <dbReference type="OrthoDB" id="6330at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000049">
        <property type="entry name" value="Proteasome subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02113_A">
        <property type="entry name" value="Proteasome_B_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019983">
        <property type="entry name" value="Pept_T1A_Psome_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF0">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE SUBUNIT HSLV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0645">Protease</keyword>
    <keyword evidence="7 9 10" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="8 9" id="KW-0865">Zymogen</keyword>
    <feature type="propeptide" id="PRO_5015793380" description="Removed in mature form; by autocatalysis" evidence="9">
        <location>
            <begin position="1"/>
            <end position="3"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023475934" description="Proteasome subunit beta" evidence="9">
        <location>
            <begin position="4"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH33376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="194" mass="20785" checksum="96BDB7728FE8C0D0" modified="2018-07-18" version="1">MPGATAVGITFDGGVVFASEKRIAFGNFLVSKSTKKTFPITNKVGAACAGLVADMQILALQISALAKIRKMELKRDVPPNTVAKMMSNMMYERRYFPLLTQVIVGGVVDKPIMYTLDPLGSVLPDEYAAVGTGAEMALGVLDPQFKPNLSKDEAIDLAKRAVRSAALRDSASGDGIDILVITKDGTEEFTEQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP29</accession>
    <name>A0A2S2KP29_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875919.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP29"/>
    <dbReference type="GeneID" id="76209363"/>
    <dbReference type="OrthoDB" id="4075at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="95" mass="10655" checksum="8FECA14A7C37F0AD" modified="2018-07-18" version="1">MTLDPEFSKQTTDLIEQTLELYKSAGASPRVGETWDCANIGDFLCGFFVGEMVGSALSAFQIVHQREPTADEHLEIIELVESHAKEIKEFFAKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP35</accession>
    <name>A0A2S2KP35_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fido domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_01930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP35"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3290.10">
        <property type="entry name" value="Fido-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003812">
        <property type="entry name" value="Fido"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036597">
        <property type="entry name" value="Fido-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040198">
        <property type="entry name" value="Fido_containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13504:SF38">
        <property type="entry name" value="FIDO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13504">
        <property type="entry name" value="FIDO DOMAIN-CONTAINING PROTEIN DDB_G0283145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02661">
        <property type="entry name" value="Fic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140931">
        <property type="entry name" value="Fic-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51459">
        <property type="entry name" value="FIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fido" evidence="1">
        <location>
            <begin position="113"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="261" mass="30988" checksum="69FA9B19A62A78DE" modified="2018-07-18" version="1">MRIPKNIEEIKKEFLLKFYHEQWDMQISAIAKNYQKETKSKPPSIKQKDFESFGISFTYNTQRMEGSSLTEADTRDLLVHGVTPTKKSQIDSIETKKHYDLFMKLVTSKNTKITKKTVMGWHKEIFDQTKIGEAGSIRTYRVGVITNDKIEFAPAHKIPERLDAFFKWLNKSKTTNFVELAALAHYYFVSIHPFGDGNGRISRLIMNYILFIHRCPFMQIKNNDRHTYFKALEKSQIKRDEIHFLKWFMRYYIKSNRNHLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP57</accession>
    <name>A0A2S2KP57_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_02100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876078.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP57"/>
    <dbReference type="GeneID" id="76209544"/>
    <dbReference type="OrthoDB" id="12289at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="472" mass="53207" checksum="E8B7728338901C9B" modified="2018-07-18" version="1">MKYFAIFLIIGGLIALFGMFAIPPAYASFTDSILVQVDDDFRKVYYNEKNVDSVLFDFDSSSVVFETGYNAVLEIKSPKVYKVGPGPFILKNGEEIAPEYTSDDCFYYINIETKTPKTIELVFTFWPEYPEIIEGCEAFIVSPLKQFKSDIPIHNIQCKEGLMLIEKYDETPACVTPDSKQKLLERHWTVSPSYKVVGLDTAYVGEPIKIAVEKLGWNKCNNYDAKIYTISHDNVWGYGSSGSCVTLDPPKLSKSTIDVPRNKKYPIIETTGNYIFEIKFGHTIITEEFSVKNLISKPTQNTISVFCGPSLEQIRAESSIDILAPRHLPVGYSLKSTDNIHPGIILLNYARGNTCGTDAHTLEDGKIEVVIAFSPDAPLESDGETFLEQYKTQYKENNVGFQTSVVDSRFYIIGVNGMIQQLVDNDRIYDEKWIEPTRVHVFDEKNSAGYKITAHLPLYEVMSIAKSLSEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP58</accession>
    <name>A0A2S2KP58_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">6-phosphogluconate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_02510</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876114.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP58"/>
    <dbReference type="GeneID" id="76209585"/>
    <dbReference type="OrthoDB" id="23890at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="HIBADH-like_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015815">
        <property type="entry name" value="HIBADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051265">
        <property type="entry name" value="HIBADH-related_NP60_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43580:SF2">
        <property type="entry name" value="CYTOKINE-LIKE NUCLEAR FACTOR N-PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43580">
        <property type="entry name" value="OXIDOREDUCTASE GLYR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000103">
        <property type="entry name" value="HIBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase NADP-binding" evidence="3">
        <location>
            <begin position="3"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyisobutyrate dehydrogenase-like NAD-binding" evidence="4">
        <location>
            <begin position="165"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="294" mass="32066" checksum="83BDD785233B9F23" modified="2018-07-18" version="1">MKKIGIIGLGMLGNAVALHLLDAGFDVTVYNRTKEKTIQVKEKGGKVASSPKETAENTELVIIVVKDANAVKQVSFGNKGIIECKNEKLIVADMSTIEPSESKNISKKFQEHNIQKLDIPVMGGPNVAITGELVMMASGDKTAFDNCKDVLEKIANKVFFLGGSGVAHSVKLAMNLQITMLALALSEGITLMKSADVDPKIFLEILNSTYFKTGMSEKKAYKMIDGEYTATFTLSNLKKDISTITKMAKSLGIKLPMIEKAEEVYDDALKEGLGEIDYTGIIEHIKRINDSKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPF0</accession>
    <name>A0A2S2KPF0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">tRNA carboxymethyluridine synthase</fullName>
            <ecNumber evidence="13">2.3.1.311</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">NZNM25_03130</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>uridine(34) in tRNA + acetyl-CoA + S-adenosyl-L-methionine + H2O = 5-(carboxymethyl)uridine(34) in tRNA + 5'-deoxyadenosine + L-methionine + CoA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:61020"/>
            <dbReference type="Rhea" id="RHEA-COMP:10407"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74882"/>
            <dbReference type="EC" id="2.3.1.311"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:61021"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="15">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ELP3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.311" evidence="13"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876166.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPF0"/>
    <dbReference type="GeneID" id="76209650"/>
    <dbReference type="OrthoDB" id="49957at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106261">
        <property type="term" value="F:tRNA uridine(34) acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034687">
        <property type="entry name" value="ELP3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056591">
        <property type="entry name" value="ELP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01211">
        <property type="entry name" value="ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF0">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23613">
        <property type="entry name" value="ELP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005669">
        <property type="entry name" value="Hist_AcTrfase_ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01086">
        <property type="entry name" value="elongater_protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00344">
        <property type="entry name" value="ELP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10 15" id="KW-0408">Iron</keyword>
    <keyword evidence="11 15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Radical SAM core" evidence="16">
        <location>
            <begin position="74"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR005669-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GBH33522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="528" mass="59876" checksum="40EEE82085262E8D" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPF1</accession>
    <name>A0A2S2KPF1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_03270</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876177.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPF1"/>
    <dbReference type="GeneID" id="76209665"/>
    <dbReference type="OrthoDB" id="43333at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="17"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="81"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="132" mass="14177" checksum="7F654C4DB4D50ED3" modified="2018-07-18" version="1">MSRQDFNSNSITVQDIMTRTMITVNSATTAQQIAKMMQQGGIGAIFVKENDNPVGIVTDRDFATKIAANGLSLDTPAKKIMSSPLITINHNEPLSAAAERMTSKKIRKLAVTDNGKIVGIVTSTDLVTQLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPI7</accession>
    <name>A0A2S2KPI7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Proline--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Prolyl-tRNA synthetase</fullName>
            <shortName evidence="8">ProRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">proS</name>
        <name evidence="10" type="ORF">NZNM25_02320</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>tRNA(Pro) + L-proline + ATP = L-prolyl-tRNA(Pro) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15" evidence="8"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876261.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPI7"/>
    <dbReference type="GeneID" id="76209566"/>
    <dbReference type="OrthoDB" id="7375at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00862">
        <property type="entry name" value="ProRS_anticodon_zinc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00778">
        <property type="entry name" value="ProRS_core_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000005">
        <property type="entry name" value="bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000037">
        <property type="entry name" value="Proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.30">
        <property type="entry name" value="C-terminal domain of ProRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01571">
        <property type="entry name" value="Pro_tRNA_synth_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002316">
        <property type="entry name" value="Pro-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004499">
        <property type="entry name" value="Pro-tRNA-ligase_IIa_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016061">
        <property type="entry name" value="Pro-tRNA_ligase_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017449">
        <property type="entry name" value="Pro-tRNA_synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033721">
        <property type="entry name" value="ProRS_core_arch_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00408">
        <property type="entry name" value="proS_fam_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382:SF2">
        <property type="entry name" value="BIFUNCTIONAL GLUTAMATE_PROLINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382">
        <property type="entry name" value="PROLYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09180">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01046">
        <property type="entry name" value="TRNASYNTHPRO"/>
    </dbReference>
    <dbReference type="SMART" id="SM00946">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64586">
        <property type="entry name" value="C-terminal domain of ProRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="3 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 8 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="9">
        <location>
            <begin position="5"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH33441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="480" mass="54819" checksum="71CF1C6226E20278" modified="2018-07-18" version="1">MSKEDIGITVTKKDDFSEWYTQVVLKAKLADYAPVKGLIVLRPDGYSIWESLRSTFDKKFAKNGIRNGFLPILIPESLLGKEQKHFAGFNPEVFWVTHSGTNEIGDKLALRPTSETLAYTMYAKWIQSWRDLPLKINFWNTALRAEIKATKPFLRTSEFLWQEGHTVHTSQEEAEEEVIKILEIYKNSVEEELAIPVTVGKKSEKEKFVGAVYTTTMESIMPDGKALQMGTSHFLGQNFSKPFEVKFADKDNVEHFAWQTSWGVSWRLIGAMIMVHGDDKGLVLPPKVAPIQVVIVPIYKNDEGKEKVLPKVEEIKNKLELKEIRVHVDNREGLSPGYKFNDWELKGVPLRIEIGPKDIEKESVVIAKRYNCEKTNLNFTDIERIVIILEEIQKEMLKNAQEQSKKNSINISDYSEFKSKIEGGGFLNAPWCGKSKCEEKIKEETGADIRVIPFGSENLNLKCIYCQEQSQSIPIFARGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPJ0</accession>
    <name>A0A2S2KPJ0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Prephenate/arogenate dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_03530</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876201.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPJ0"/>
    <dbReference type="GeneID" id="76209693"/>
    <dbReference type="OrthoDB" id="24743at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008977">
        <property type="term" value="F:prephenate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004665">
        <property type="term" value="F:prephenate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3660.10">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046825">
        <property type="entry name" value="PDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046826">
        <property type="entry name" value="PDH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050812">
        <property type="entry name" value="Preph/Arog_dehydrog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003099">
        <property type="entry name" value="Prephen_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21363">
        <property type="entry name" value="PREPHENATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21363:SF0">
        <property type="entry name" value="PREPHENATE DEHYDROGENASE [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20463">
        <property type="entry name" value="PDH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02153">
        <property type="entry name" value="PDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51176">
        <property type="entry name" value="PDH_ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate/arogenate dehydrogenase" evidence="2">
        <location>
            <begin position="2"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="283" mass="31645" checksum="A95D1D1B7EB0455D" modified="2018-07-18" version="1">MKKMTVVGAGGQMGQWFARYFADKGFEVTGYDSENKITGKNIIASDSLVGSILKADYVVLCTPTRRTPEIIRLIAKEMKRGTYLIEISSEKSKVVSSLSKMPDKINPICIHPMFGPGTKIIKGQNIISVPIKDAKKELTVAKSLFDGANFVTIDAAEHDKKIAVILGLTHLMNLVFANIISKDEKMNLTEKMSGTTFRVQKTLAESIMTESPELIETIIANPEIRRVAEELWKDIGRLLTAVQESKTEEVTTYIRECQERLAKHTDINESYKKLTKMVKSVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPJ3</accession>
    <name>A0A2S2KPJ3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">H/ACA RNA-protein complex protein Gar1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03810</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_264953658.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPJ3"/>
    <dbReference type="GeneID" id="76209723"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.230">
        <property type="entry name" value="Probable tRNA pseudouridine synthase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038664">
        <property type="entry name" value="Gar1/Naf1_Cbf5-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="9012" checksum="B8EE07C602425B7F" modified="2018-07-18" version="1">MHLAGSGRVIIQLSKELVEGQILCDEKGTRVAKVNEMIGPVSRPYASATPLTNSIKKYIGKSVYAIEQSPVNKPKKFRRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPK0</accession>
    <name>A0A2S2KPK0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Short-chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_02520</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876115.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPK0"/>
    <dbReference type="GeneID" id="76209586"/>
    <dbReference type="OrthoDB" id="24596at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005559">
        <property type="entry name" value="PRK07231.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF115">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE FABG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000126">
        <property type="entry name" value="11-beta-HSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH33461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="253" mass="27156" checksum="DF5A581EA81C97BA" modified="2018-07-18" version="1">MRLSDKVAIVTGASSDIGKGIAKRFSEEGAKVVMVARNLEGLENARKEIGNEESTASVTCDLTDESQVLQAVNQIMDTYGKIDILVNNAGAINDPVHFHEMKDAEIKKLIDVNLLGVFHMTKAVLSKMSDVKSGAIVNIGSISSERAIPRVHLAVYSATKAAISMFTKSIAVEYARRNIRCNCVNPGIINSGMIKPYLDDPQARKVLEERLPLARVGEPADVANAALYLASDEANWVTGTILNVDGGKTASEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPP8</accession>
    <name>A0A2S2KPP8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Thaumarchaeosortase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_02460</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPP8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026392">
        <property type="entry name" value="Exo/Archaeosortase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027561">
        <property type="entry name" value="Thauma_sortase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04178">
        <property type="entry name" value="exo_archaeo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04297">
        <property type="entry name" value="thauma_sortase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="216"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="285" mass="32701" checksum="10175E679A2016CA" modified="2018-07-18" version="1">MIAFPDSIAWSWNEGRGGYFFALVFIVAELIGLKIVISKKRLFSVIPLAFLTIAYLISLEYGLRDYIIESAEQFDVQLIYSWTWMWDFVIMAIFVVVALTIFFGKRWIRIAPAGPIFLTGTALILSLDAFFPYDTLGPLQYVVPYFVQTNVWVITMLDLGTAIAKDNVMFLRGEHGSMALQVFWPSAGVHSIIIFSLVIGAFMLKLNIPRHRKAMYFVLGIIGTITVNLIRIFSLSWYALKVTTDPVAWEEYHKIAGEIMFLPWLFAFILVVILIESRRMKKREQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPQ9</accession>
    <name>A0A2S2KPQ9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04430</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876315.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPQ9"/>
    <dbReference type="GeneID" id="76209793"/>
    <dbReference type="OrthoDB" id="2724at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="77" mass="9081" checksum="9ED7D5CE76C5A26C" modified="2018-07-18" version="1">MQVYVRKEIQAFEYVSFTEFKNEDELDFCCEKLKKYVSVVKSWSSKIGKFCIVEKDILTPIDYCPFCGAKIEYISVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPV0</accession>
    <name>A0A2S2KPV0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_05010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_160049191.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPV0"/>
    <dbReference type="GeneID" id="76209853"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="57" mass="5847" checksum="1E0F211EBD059DBE" modified="2018-07-18" version="1">MITEDAGLVLAIFILAVGFIATLIGVANLFDSEPNPLIFVGGISALIIGGILVKFST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPV6</accession>
    <name>A0A2S2KPV6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Roadblock/LC7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_02890</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876144.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPV6"/>
    <dbReference type="GeneID" id="76209626"/>
    <dbReference type="OrthoDB" id="1979at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046600">
        <property type="entry name" value="DUF6659"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20364">
        <property type="entry name" value="DUF6659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="124" mass="13880" checksum="F3A8AF16E4476610" modified="2018-07-18" version="1">MAINYDELSKKVLALDSQIRFAGIANSKGELVAGGHKESIDGMLSDDEVKMSIHYALQKRELYTNLAYKIGHETSSITEYEKVTMISVPINSNELFMVSTEPRADYLKIIDYIHSALGSEKYSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ29</accession>
    <name>A0A2S2KQ29_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rps12</name>
        <name evidence="10" type="ORF">NZNM25_05640</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">With S4 and S5 plays an important role in translational accuracy. Located at the interface of the 30S and 50S subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7 9">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876420.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ29"/>
    <dbReference type="GeneID" id="56066892"/>
    <dbReference type="OrthoDB" id="45154at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03367">
        <property type="entry name" value="Ribosomal_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000007">
        <property type="entry name" value="40S ribosomal protein S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00403_A">
        <property type="entry name" value="Ribosomal_uS12_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_uS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022863">
        <property type="entry name" value="Ribosomal_uS12_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005680">
        <property type="entry name" value="Ribosomal_uS12_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003254">
        <property type="entry name" value="PRK04211.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00982">
        <property type="entry name" value="uS12_E_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002133">
        <property type="entry name" value="Ribosomal_S12/S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7 9" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH33773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="145" mass="15940" checksum="5B16C2C266D06ACE" modified="2018-07-18" version="1">MRKSPLGLFAGRVLTTKKKRQRWAISTYKRRKLGIDKKADPLGGAPQGRGIVLEKVGIAAKQPNSAIRKCVRVQLIKNGKTVTAFLPRDGAMNFIDEHDEVHIQGMGATQGGAMGDIPGVRFKVFKVNGTSLHELVIGKKEKPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ42</accession>
    <name>A0A2S2KQ42_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Endoribonuclease Nob1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_04580</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Toxic component of a type II toxin-antitoxin (TA) system. Processes pre-16S-rRNA at its 3' end (the D-site) to yield the mature 3' end.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NOB1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876328.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ42"/>
    <dbReference type="GeneID" id="76209810"/>
    <dbReference type="OrthoDB" id="27944at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09876">
        <property type="entry name" value="PIN_Nob1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000020">
        <property type="entry name" value="20S-pre-rRNA D-site endonuclease NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039907">
        <property type="entry name" value="NOB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033411">
        <property type="entry name" value="Ribonuclease_PIN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814:SF2">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17146">
        <property type="entry name" value="PIN_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="domain" description="Ribonuclease PIN" evidence="8">
        <location>
            <begin position="5"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="159" mass="17413" checksum="DC55CC1C7F7C8872" modified="2018-07-18" version="1">MGFRILDASAFYAGVPFGSADDCYTTSLVFEEIKHIKKNQDALGTLLETNRLKIREPDKESTVAAVKAARDTGDYQQLSKQDVSIIALCIEMNGEIISDDFAISNVARNLGLKISPIMTQGITDVGKWVHYCPGCRTNHTSGNECPMCGTPLKRKLLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ44</accession>
    <name>A0A2S2KQ44_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829030.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ44"/>
    <dbReference type="GeneID" id="76209780"/>
    <dbReference type="OrthoDB" id="144at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="55" mass="6383" checksum="75849ED33B940282" modified="2018-07-18" version="1">MCECSKVHLFEVEFKLDGMTVVPTHKNCGFGLDEKQADKFQKELVKSWGFEEDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ57</accession>
    <name>A0A2S2KQ57_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-directed RNA polymerase subunit Rpo10</fullName>
            <ecNumber evidence="5">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">DNA-directed RNA polymerase subunit N</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpo10</name>
        <name evidence="5" type="synonym">rpoN</name>
        <name evidence="6" type="ORF">NZNM25_05970</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal Rpo10/eukaryotic RPB10 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="5"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ57"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000024">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00250">
        <property type="entry name" value="RNApol_arch_Rpo10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023580">
        <property type="entry name" value="RNA_pol_su_RPB10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020789">
        <property type="entry name" value="RNA_pol_suN_Zn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000268">
        <property type="entry name" value="RPABC5/Rpb10"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003089">
        <property type="entry name" value="PRK04016.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431:SF3">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01194">
        <property type="entry name" value="RNA_pol_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46924">
        <property type="entry name" value="RNA polymerase subunit RPB10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01112">
        <property type="entry name" value="RNA_POL_N_8KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 5" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="90" mass="10674" checksum="F8141D10B6D9BBAE" modified="2018-07-18" version="1">MVFIILDYFFVRMLIPVRCFTCGNLVADKFEDYQNKIKSGEDPVKTLDSLGIERYCCRRMLLTTVETIQQVIPFYEAIQRRKQEVQSELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ76</accession>
    <name>A0A2S2KQ76_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_03990</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876281.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ76"/>
    <dbReference type="GeneID" id="76209745"/>
    <dbReference type="OrthoDB" id="10612at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="115" mass="13275" checksum="06B0966A3B7A126F" modified="2018-07-18" version="1">MSDFTPTIPISLQIRKIIFEKFNDPDGKFTNDEIFEIIKENGDLDPSWVIDDTESFFNELCDSGLVRNIAQNFTTIWMKLFDPVEKFHCNSCNHDVYLGISEERICPNTSCKSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ80</accession>
    <name>A0A2S2KQ80_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04980</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876363.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ80"/>
    <dbReference type="GeneID" id="76209850"/>
    <dbReference type="OrthoDB" id="2634at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="419" mass="48555" checksum="753ED5ABA602B331" modified="2018-07-18" version="1">MALSSSVIYALKLLKPDSINSVFLSFFEYFKYDSDEIRTQQIILKEDILEQFKKSDRQKGKTLPKSLSEDFDELVKILSKLRLLEEDGDVLQNIKWDKIIDVCNILRKIESSDERKVRWGLLHLLSHDKDEININELKLLLKNFKLTNIDKILQKIIKIESNSGLMITNDNNKIKFETEHKITDHYIADAIEEKSRRSPGEIEKDILELLDEGSYSNQEISRILELDEAVVSRTMTKLRNQNKLVLSNFGTNGFRYYITNCQNCPFGKTVSSCRKDAISYIVSAFKEAYDVELTSQHFDDVESNQALLSIKRITKMSKKYSMTKFESNLYENYEKLLQIVIKNSTKKTMNAGNNDEIAVLPNLSKLPKIFQIGFKSGIQYEVQLMKKILNDPKKKQSDFASFLSKLIQDSNKMLHLFGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQI7</accession>
    <name>A0A2S2KQI7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable tRNA pseudouridine synthase B</fullName>
            <ecNumber evidence="5">5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">tRNA pseudouridine(55) synthase</fullName>
            <shortName evidence="5">Psi55 synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">tRNA pseudouridylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">tRNA-uridine isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">truB</name>
        <name evidence="8" type="ORF">NZNM25_05090</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 5">Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>uridine(55) in tRNA = pseudouridine(55) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10102"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the pseudouridine synthase TruB family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="5"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876371.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQI7"/>
    <dbReference type="GeneID" id="76209860"/>
    <dbReference type="OrthoDB" id="35866at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160148">
        <property type="term" value="F:tRNA pseudouridine(55) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000495">
        <property type="term" value="P:box H/ACA sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031118">
        <property type="term" value="P:rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21148">
        <property type="entry name" value="PUA_Cbf5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.10:FF:000001">
        <property type="entry name" value="H/ACA ribonucleoprotein complex subunit CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01081">
        <property type="entry name" value="TruB_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012960">
        <property type="entry name" value="Dyskerin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004802">
        <property type="entry name" value="tRNA_PsdUridine_synth_B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026326">
        <property type="entry name" value="TruB_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00425">
        <property type="entry name" value="CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003280">
        <property type="entry name" value="PRK04270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127">
        <property type="entry name" value="CENTROMERE/MICROTUBULE BINDING PROTEIN CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127:SF0">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT DKC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08068">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01136">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Dyskerin-like" evidence="7">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="PUA" evidence="6">
        <location>
            <begin position="241"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00060072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00060775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="333" mass="37403" checksum="EC7A3A257D4D52C2" modified="2018-07-18" version="1">MTLKQLENLIEIDQDITDDAYGTPYDKRTLEQLLNYGVILLDKPPGPTSHETVAWAKRILKLPKIGHSGTLDPQVSGVLPLGLGEATKALGVLLLGPKEYVALGRVHSLPSKEKLSEIINLFKGEIFQKPPQRSAVVRQTRTRTIYEFELIEQKERLLLTRVLCEAGTYIRKLYYDIGEILGPGATMIELRRTRVDQFYETDGLVTLHELANAFALWEENKDETRLMQMIKPVEYALSELKSVVIRDSAVDAMCHGAQLAIPGVLKISPNLKKGDLVGIYTQKGEAVALAESTMSEEEIRDATKGYAFETKRIIMAPNTYPKKWRTKPTHSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQJ1</accession>
    <name>A0A2S2KQJ1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper-containing nitrite reductase</fullName>
            <ecNumber evidence="3">1.7.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">NZNM25_05060</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>nitric oxide + Fe(III)-[cytochrome c] + H2O = Fe(II)-[cytochrome c] + nitrite + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16301"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.7.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>Cu(+)</name>
            <dbReference type="ChEBI" id="CHEBI:49552"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotrimer.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.7.2.1" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQJ1"/>
    <dbReference type="OrthoDB" id="12293at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050421">
        <property type="term" value="F:nitrite reductase (NO-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd11024">
        <property type="entry name" value="CuRO_1_2DMCO_NIR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000923">
        <property type="entry name" value="BlueCu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052721">
        <property type="entry name" value="ET_Amicyanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001287">
        <property type="entry name" value="NO2-reductase_Cu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507:SF1">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00127">
        <property type="entry name" value="Copper-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00695">
        <property type="entry name" value="CUNO2RDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 10" id="KW-0186">Copper</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="13">
        <location>
            <begin position="48"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="12">
        <location>
            <begin position="182"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Blue (type 1) copper" evidence="11">
        <location>
            <begin position="367"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="type 1 copper site" evidence="10">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601287-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GBH33715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="442" mass="49782" checksum="FD08FC3004CE334B" modified="2018-07-18" version="1">MLIGVVSLGVALGIFFLNTEIPLQSFAEDNSVHNSPVTRTYTVVAEDTTLEISPGLRVEAWTYNGTIPGPTLRATEGDRVIINFINNGQLPHTMHFHGDHDEKNDGVFQEVLPGESYTYDFTAEPAGLFMYHCHVMPVSEHIRNGLYGAFIVDPKEGLEPAREYVLVKGEYDLEDQETWTPDYVFFNGYADQYWTNPLPAKTNELVRLYYVDMGAIPAFGFHIHGTIFETIVSGIWENAPIKTQTWEVGPGNAAIFEAKWKDPGRYLFHLHGIPEEKGTMAYFDVRDADSDAIDGVDIAKTKSIDMWDWQKQILTDLEMPDDDAEITKTIASTSSHEEHLTFAQNTDESHIVETTLCEVEEGSAIKSSNKSYYPKLTQIQVGDTVTWTNKDVSVHTVTSNDELFDSGMMMPGDTFEQTFESVGLYEYYCMLHPWMTGTIKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQL0</accession>
    <name>A0A2S2KQL0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Excinuclease ABC subunit C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_07370</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876598.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQL0"/>
    <dbReference type="GeneID" id="76208939"/>
    <dbReference type="OrthoDB" id="121419at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10434">
        <property type="entry name" value="GIY-YIG_UvrC_Cho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1440.10:FF:000001">
        <property type="entry name" value="UvrABC system protein C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.10">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.10">
        <property type="entry name" value="UVR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.340">
        <property type="entry name" value="UvrC, RNAse H endonuclease domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047296">
        <property type="entry name" value="GIY-YIG_UvrC_Cho"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036876">
        <property type="entry name" value="UVR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050066">
        <property type="entry name" value="UvrABC_protein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004791">
        <property type="entry name" value="UvrC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001162">
        <property type="entry name" value="UvrC_RNase_H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038476">
        <property type="entry name" value="UvrC_RNase_H_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00194">
        <property type="entry name" value="uvrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30562:SF1">
        <property type="entry name" value="UVRABC SYSTEM PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30562">
        <property type="entry name" value="UVRC/OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01541">
        <property type="entry name" value="GIY-YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22920">
        <property type="entry name" value="UvrC_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08459">
        <property type="entry name" value="UvrC_RNaseH_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00465">
        <property type="entry name" value="GIYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46600">
        <property type="entry name" value="C-terminal UvrC-binding domain of UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50164">
        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50165">
        <property type="entry name" value="UVRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0228">DNA excision</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIY-YIG" evidence="7">
        <location>
            <begin position="12"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="UVR" evidence="6">
        <location>
            <begin position="200"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="UvrC family homology region profile" evidence="8">
        <location>
            <begin position="288"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="528" mass="60716" checksum="68DB52EEDBAA8201" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQM5</accession>
    <name>A0A2S2KQM5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Restriction endonuclease type IV Mrr domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_07630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQM5"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007560">
        <property type="entry name" value="Restrct_endonuc_IV_Mrr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04471">
        <property type="entry name" value="Mrr_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Restriction endonuclease type IV Mrr" evidence="1">
        <location>
            <begin position="173"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="284" mass="33213" checksum="4837E97EFD56BD87" modified="2018-07-18" version="1">MNLQNELELKTFVSKILSDEDVDHKLRKYQKVLLSLEDVNKKGKSIIVSKYEEFVTQLQKQLQIKPLLIKADWKNEEKEEVMFAMKYHKFREEFEIVVMPTWSEIGIVSKLYNEYLNEQINDKIKNLTQPQSVVLIENVLSDSKIEWIKDFRLNPKASDDEGTDFTAVLYIDRDGSISHDEFGKWVEIVGQLKHLKGEMGPSDMRDFIGTIKTSKKKHGMVISTNGFTKRTIDAVDSSKFTIFCYDSNFITDLIIKHKIGMKEYRIKAGLIPDDEWWDEIRIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQT5</accession>
    <name>A0A2S2KQT5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876670.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQT5"/>
    <dbReference type="GeneID" id="76208850"/>
    <dbReference type="OrthoDB" id="8113at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="115" mass="13180" checksum="5F9F1A2395358417" modified="2018-07-18" version="1">MAISKETKDFLDSLIDYYINESESYRQIADNFVPEIESVPDTAFGIITGCVYSGFLQAYQNQQQTPSLEDMKEFNKIIKERAPLIKKAILEPRKFEDSKKETDDNQDITTDNSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQV3</accession>
    <name>A0A2S2KQV3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_08270</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876674.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQV3"/>
    <dbReference type="GeneID" id="76208846"/>
    <dbReference type="OrthoDB" id="8709at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="8">
        <location>
            <begin position="33"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="7">
        <location>
            <begin position="186"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="344" mass="37794" checksum="87577FCBD929C335" modified="2018-07-18" version="1">MEKMRAMVLFECAKIETNPLKLTEIDRHEIQRSNEILLKIEACGVCHSQLHGIEGDWKDIGIPPMLPTVPGHELVGKVIQIGDAVTKFKVGDRAGITPLLEACKECQYCKEGKEYLCESSIITGESFKGGYTEYITVTEDFAIKVPQHMKPEYAAPLFCAGITAYKAVKAAEPKSHKKIGIFGIGGVGHMAVQFAKVENCDVIAFSRTQKHLDVAKRLGAIDAMTFSENQEEFLNKLKNKHGMLDAAIVFAPSDIATDTAIKSVKKGGLIVIATVGKNPSFMAFEEKTIRGTLIGSTKDMEQVIKICDENNIEVISQVFPLENANKVLKKLKDSEIEARAVLIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQW1</accession>
    <name>A0A2S2KQW1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">UvrABC system protein A</fullName>
            <shortName evidence="14">UvrA protein</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Excinuclease ABC subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">uvrA</name>
        <name evidence="16" type="ORF">NZNM25_07380</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Forms a heterotetramer with UvrB during the search for lesions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the ABC transporter superfamily. UvrA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876599.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQW1"/>
    <dbReference type="GeneID" id="76208938"/>
    <dbReference type="OrthoDB" id="7896at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03270">
        <property type="entry name" value="ABC_UvrA_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03271">
        <property type="entry name" value="ABC_UvrA_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000028">
        <property type="entry name" value="UvrABC system protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.280">
        <property type="entry name" value="ABC transporter ATPase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1580.10">
        <property type="entry name" value="ABC transporter ATPase like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00205">
        <property type="entry name" value="UvrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004602">
        <property type="entry name" value="UvrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041552">
        <property type="entry name" value="UvrA_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041102">
        <property type="entry name" value="UvrA_inter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001503">
        <property type="entry name" value="PRK00349.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00630">
        <property type="entry name" value="uvra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43152">
        <property type="entry name" value="UVRABC SYSTEM PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43152:SF3">
        <property type="entry name" value="UVRABC SYSTEM PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17755">
        <property type="entry name" value="UvrA_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17760">
        <property type="entry name" value="UvrA_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 14" id="KW-0228">DNA excision</keyword>
    <keyword evidence="13 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 14" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="3 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0742">SOS response</keyword>
    <keyword evidence="9 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="604"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="14">
        <location>
            <begin position="252"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="14">
        <location>
            <begin position="737"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="33"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="638"/>
            <end position="645"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GBH33947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="941" mass="104887" checksum="1B3095767E5ACF34" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQZ3</accession>
    <name>A0A2S2KQZ3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GTP-dependent dephospho-CoA kinase</fullName>
            <ecNumber evidence="6">2.7.1.237</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Dephospho-coenzyme A kinase</fullName>
            <shortName evidence="6">DPCK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_08730</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the GTP-dependent phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A (CoA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3'-dephospho-CoA + GTP = GDP + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61156"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="2.7.1.237"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GTP-dependent DPCK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.237" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQZ3"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00590">
        <property type="entry name" value="Dephospho_CoA_kinase_GTP_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007164">
        <property type="entry name" value="GTP-dep_dephospho-CoA_kin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40732:SF1">
        <property type="entry name" value="GTP-DEPENDENT DEPHOSPHO-COA KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40732">
        <property type="entry name" value="UPF0218 PROTEIN TK1697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04019">
        <property type="entry name" value="DUF359"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006533">
        <property type="entry name" value="UCP006533"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="164" mass="18257" checksum="269EC4D96836189B" modified="2018-07-18" version="1">MKLPESLRDQLKVPLGLLLPESQADKTNIQKHLTENSYIITVGDRTTEKMIEFGLIPSLQIIDNLEKRAKRKPPKLESAIELTVDNPPAEITSNSMDVIKKAFAMKPPIRLTVNGEEDLLVLPVCIYAPENATVLYGQPNEGLVIVTITPEIRNKAQSLLDSME</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR21</accession>
    <name>A0A2S2KR21_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tubulin/FtsZ GTPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_09210</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876756.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR21"/>
    <dbReference type="GeneID" id="76208749"/>
    <dbReference type="OrthoDB" id="10400at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="CELL DIVISION PROTEIN FTSZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314:SF3">
        <property type="entry name" value="MITOCHONDRIAL DIVISION PROTEIN FSZA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="3">
        <location>
            <begin position="8"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="314" mass="33851" checksum="E78772141DA21965" modified="2018-07-18" version="1">MSFQVKEPILVIGLGGIGSKLAIKAKDSLNSDCLLISNDEKDFTSGNPSVHVSTKGVVNPSVQLIRGSAYNVSDEIKSKIEEYSTIILMSNLAGKAGSAIAPVVSEMCKDKGLVSFAIMPFKYEKDRIFNSGISLKRIRENSKCTVVLDNDSLLESNPDLSPKTCYDIANSAIMHVVESIGSSEISNETNILTTSKAGQNIEDSLRDSLKMLYGNAPPNAVKRSMLYVVGGGNIPVGILNSITNITGGILNDANSQIDMSSTSEESKVVMLSSIQGMTKFDNYDPLGMIPQEDTLDWSTPDCSIDCKLDLYQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR70</accession>
    <name>A0A2S2KR70_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Sulfonate ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_09440</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876778.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR70"/>
    <dbReference type="GeneID" id="76208726"/>
    <dbReference type="OrthoDB" id="159847at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13946">
        <property type="entry name" value="LysW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005906">
        <property type="entry name" value="LysW"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041070">
        <property type="entry name" value="carrier_LysW_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40393:SF2">
        <property type="entry name" value="ALPHA-AMINOADIPATE_GLUTAMATE CARRIER PROTEIN LYSW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40393">
        <property type="entry name" value="LYSINE BIOSYNTHESIS PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21344">
        <property type="entry name" value="Zn_ribbon_LysW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="55" mass="5822" checksum="9C4494CC9CB812E9" modified="2018-07-18" version="1">MSKCEECDAEISVPSDALEGEIVTCPECGASFELVKGSDGFQLKPAQTVGEDWGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR85</accession>
    <name>A0A2S2KR85_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Blue (type 1) copper domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_09540</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876787.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR85"/>
    <dbReference type="GeneID" id="76208716"/>
    <dbReference type="OrthoDB" id="11836at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000923">
        <property type="entry name" value="BlueCu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052721">
        <property type="entry name" value="ET_Amicyanin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507:SF1">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00127">
        <property type="entry name" value="Copper-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Blue (type 1) copper" evidence="3">
        <location>
            <begin position="97"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="173" mass="19371" checksum="CDEADE52A3241C0A" modified="2018-07-18" version="1">MEHFPKKTTLPIIVCSILLISVSANAANAESVPEWVKNTALWYGQGIVSEQEFLNMIKFLIDNDVITIEAQEEISTKKDSTITIPNGNFNVSSTSFYLPLNLEIPVGTTVTWVNNDSVPHNIQSQDDSGKVIDMFNSPPLSTGDKFSYTFEEDGIFKYHCSWHPWRVGLVTVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRB2</accession>
    <name>A0A2S2KRB2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NurA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08980</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876736.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRB2"/>
    <dbReference type="GeneID" id="76208771"/>
    <dbReference type="OrthoDB" id="2179at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018977">
        <property type="entry name" value="NurA_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09376">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NurA" evidence="1">
        <location>
            <begin position="53"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="365" mass="40949" checksum="0290F2DA497752EA" modified="2018-07-18" version="1">MSEDPVKNMIAELGAHLSQKEHSDIVLNNGNGKQFLIAPSEFVEIKSIDSPRKIAFVDGGDGPLEESPNFLITINRVYFSLFQGKKRIKPKANPRVQFFSSVTSNITTVKGKKEVSYNTRLFPHSTEDKKYLPDESDLTSSTESTSVLQGARLNSLGRRFAEWQLAIHVVESELEKGDMLVMDGSLQTSFKNETKYANRLYDLAISKGVIVCGLAKTSRLITESGHPLLARVAEIAEDVPFGRWYVKVAEEVSGDDRGFMLAVKFHEKSRYVFRFEILREQFSLMTPDDLNSVLGSLAENSQDVAMIGYPYGAIDADRFAQVRRDELGMYQGFILSEKLRDPIWKRLQKYSASLSAHDTLNGVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRC7</accession>
    <name>A0A2S2KRC7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Plastocyanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_09940</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866850.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRC7"/>
    <dbReference type="GeneID" id="76208673"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="238" mass="27114" checksum="9563ACA1AEA3D32C" modified="2018-07-18" version="1">MKKILIGLAFAILLTTSYVPNSFSQDAVPQWIKQTAGWYSDGLVSEKEFLDAIRFLVQNKIILLDETVLDDPTLLTNDIVVSKPKIRQCEVLFQSYKNIGPLQFKSKYSYINYIDSCMKLYKDPIWSYLGSDRYDKLSERLTELNEKPPEKKLSYEPSANILAKTNLGPEKYNVKFNICAGDKAIDKAKVLVKSQIEAIQVGSNKDVPPNACRTYETLIFAKNPDNIFIEILEQVLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRD1</accession>
    <name>A0A2S2KRD1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_10040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876827.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRD1"/>
    <dbReference type="GeneID" id="76208663"/>
    <dbReference type="OrthoDB" id="6074at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="68" mass="7532" checksum="395EB37C03810CA3" modified="2018-07-18" version="1">MNIYDTKSVICAKCKSSIGEIEFDAEVIMPLCGKCANPLPEGDNIIYTINNIQNNSQQLLLKSKSVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRD9</accession>
    <name>A0A2S2KRD9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ABM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876730.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRD9"/>
    <dbReference type="GeneID" id="76208777"/>
    <dbReference type="OrthoDB" id="10504at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007138">
        <property type="entry name" value="ABM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03992">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABM" evidence="1">
        <location>
            <begin position="5"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="120" mass="14053" checksum="57C7A3AC13A07103" modified="2018-07-18" version="1">MSSQIHIKAEIIIKKEKINEFKKLIRKMSKLVKTNEPNTLQYSFYLSDDNTRCIVYERYADSKATIEHNNGVASKTMLPQIFQISKLNRLEVYGKPSKELQKILTNFNAKIYDFVTGFDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRE8</accession>
    <name>A0A2S2KRE8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_10330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876853.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRE8"/>
    <dbReference type="GeneID" id="76208632"/>
    <dbReference type="OrthoDB" id="2910at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="98" mass="11499" checksum="15AFB050846E3BE6" modified="2018-07-18" version="1">MASIIVIPVILVAIIGLSGYLVYRFLIYDLYCKRSVNQTLLKYNIKKTPSQIIKEYYHSKGESISHKEIQILEKNYRQNEPEQFLAMYDALRNQSKDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRG4</accession>
    <name>A0A2S2KRG4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytidyltransferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_09480</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRG4"/>
    <dbReference type="OrthoDB" id="1912at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050385">
        <property type="entry name" value="Archaeal_FAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43793">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43793:SF1">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="3">
        <location>
            <begin position="69"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="205" mass="22878" checksum="10D40ABC5298A77D" modified="2018-07-18" version="1">MESIDKLILSAMYVCQIDGRSVSEEIKKKSMLSDDMIKSKIDELVKNQFVNEDRTTLTEIGRSSLRVVLAGGVFDIIHPGHIHTLNAARALGDVLVVVVATDNTAVKMKKRNPIHSQEQRQELVNSLSMVDLCLIGQEDDIFKTVNMVKPEIVALGYDQVHQEKFIIEGCKKINLDAKVARLQSPIPESSSHKIEKEYGESIHGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRG5</accession>
    <name>A0A2S2KRG5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription initiation factor IIB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tfb_5</name>
        <name evidence="9" type="ORF">NZNM25_10300</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TFIIB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRG5"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20550">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000023">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Cyclin-like" evidence="8">
        <location>
            <begin position="106"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="8">
        <location>
            <begin position="200"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="284" mass="31320" checksum="F31D8518E1357409" modified="2018-07-18" version="1">MLTDDTTGEQFCSKCGYVVSEKTQESGPEWRSFQKDGGADPARTGAPSSLLMHDMGLSTVINPLNKDASGKPLSTSMKSTIERLRTWDNRSQVHEPVDRNLRQALGELTRLKDKIAISANVLEKAAYIYRKALEKKLVRGRSISAMIAASLYAACRDTATPRTLKDVADAANVKRKDIARCYRLLHYELELKMPVVDSVQCIARISSKLDITEKTKRYAIKVLKEAQEREESAGKDPMGLAASALYLSCVHNGASVTQRDIAEAAGVTEVTIRNRYKGLKASHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRL9</accession>
    <name>A0A2S2KRL9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_10080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866854.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRL9"/>
    <dbReference type="GeneID" id="76208659"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="304"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="374" mass="41343" checksum="307F44CB3482FDE6" modified="2018-07-18" version="1">MALLVLGFPYASAAQLDAAILSDQNTTEPSFQFLRVVYIEYPDEGKISELLRGTKKTVSFVADSNTPEMDELVELINQNLKKLSSNAVATDAKISYQAILQGNAHSAVIEYKVQLVPTLTNHILEKSFEKSTVDSNWRGISLDSPVVVQTIHGAFDVNNPKSALDVMIPDVSDKLKDVTILELPLIDASGIRTLPLHKWHSLFDNTAIMSDAIRYDYAGKNVITHYSMGECNIEVGMCEDKKWMQEITLDKKYVVKVIESSDDATISFEGYVDTTRLNGNEVFQTSLKSLVTHKPDTDEFPATVMYGMAGMAVIGGIVMFVISDRKLKRIKDEGQTGVDPAHLTSYEIGSSAGSYKTNRGESHLIAYEKSKMPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRM8</accession>
    <name>A0A2S2KRM8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo4</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit F</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo4</name>
        <name evidence="1" type="synonym">rpoF</name>
        <name evidence="2" type="ORF">NZNM25_10550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit is less well bound than the others.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex. Forms a stalk with Rpo7 that extends from the main structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic RPB4 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRM8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="entry name" value="HRDC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00864">
        <property type="entry name" value="RNApol_arch_Rpo4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005574">
        <property type="entry name" value="Rpb4/RPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010924">
        <property type="entry name" value="Rpo4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39646:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT RPO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39646">
        <property type="entry name" value="RNA POLYMERASE RPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03874">
        <property type="entry name" value="RNA_pol_Rpb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005053">
        <property type="entry name" value="RNA_pol_F_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="110" mass="12886" checksum="B06F11E4C5DC12C6" modified="2018-07-18" version="1">MYDMEEVQKKQAISLSEVKEILGKVDPEEMDQIQRWTYDYVSKFASIEPKDAKEMKKQLIKECELTEDEAVEIVNIRPTSLAELRSFTFGWKKLILAETLEKMLKIIKGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRN3</accession>
    <name>A0A2S2KRN3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DM13 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_09920</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876819.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRN3"/>
    <dbReference type="GeneID" id="76208675"/>
    <dbReference type="OrthoDB" id="306569at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019545">
        <property type="entry name" value="DM13_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10517">
        <property type="entry name" value="DM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51549">
        <property type="entry name" value="DM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="DM13" evidence="2">
        <location>
            <begin position="69"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="171" mass="18782" checksum="71F617AF11DDF4DB" modified="2018-07-18" version="1">MNKSIPIIIAVAIVGGISAYAVSPYFLESTIDEALPTNVVLQPKDETMMDENMMDENMMDETVPMSYAGIFIGVGDGIHDAQGDAYTIPLEDKNNILRLENFESTNGPDLYVYLSTDDNASDIVNLGKLKANKGNQNYDIPEGTDLQKYNKVLIWCKTFSVLFGSAGLSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRS2</accession>
    <name>A0A2S2KRS2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CxxC-x17-CxxC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_11470</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876988.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRS2"/>
    <dbReference type="GeneID" id="76208509"/>
    <dbReference type="OrthoDB" id="908at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026363">
        <property type="entry name" value="CxxC-x17-CxxC_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04272">
        <property type="entry name" value="cxxc_cxxc_Mbark"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23477">
        <property type="entry name" value="zf_Tbcl_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxxC-x17-CxxC" evidence="2">
        <location>
            <begin position="48"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="234" mass="26862" checksum="8F0AF967B6C2E8F8" modified="2018-07-18" version="1">MELYKSKYSDKKDSRRTSKRENNPSRSFRNSSNDRSSRYSRDDKRGESTTVTCADCGTECQIPFVPRTNKPVYCSDCFRQNKPQDSNNDRYSRDDRSSRSYNHQEFTRTDSRSKKSKGDKFLKKQESFYSGGSEKFYATLKEKLFEILGGKSCSSCGFKDERALGISHIFDDSPDSLGRGGAASSWGKYISEPELAKKDLRVLCLNCNEIRQPVSKPKENSSKSKPKKSRYFPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRT4</accession>
    <name>A0A2S2KRT4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876997.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRT4"/>
    <dbReference type="GeneID" id="76208495"/>
    <dbReference type="OrthoDB" id="2187at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="120" mass="12628" checksum="3A30B27006EDF9E1" modified="2018-07-18" version="1">MTLPKGFGSGGTGGSSSADIERMIGRRVENMTGMITVSFWAALLATFGGTAAGYFYYPWAYPTASGHFAFIVLAIIEAIGYIFCVKVMEEGSNKNSNGIVAASIAGATAFVLFVSLYVGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS13</accession>
    <name>A0A2S2KS13_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_12370</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877083.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS13"/>
    <dbReference type="GeneID" id="76209267"/>
    <dbReference type="OrthoDB" id="24644at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015854">
        <property type="entry name" value="ABC_transpr_LolD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24220">
        <property type="entry name" value="IMPORT ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24220:SF659">
        <property type="entry name" value="TRANSPORTER, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="21"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="276" mass="30794" checksum="3DB386C0237E535C" modified="2018-07-18" version="1">MKQIQMTKNPSFSSISTNKIIQMNNVSTSYDSKNFALEGINLSIDRGTNYAIVGQSGSGKSTLLKLMNGMMIPSKGTIKIDYTTPNMNNKKFKKMMHEIGYIPQSLGLVKNISVFENIMIGALPRLSKIQSLLKKFPDGEIQEAMKIIDQVGLSGKETRKAYMLSGGEKRRVAIARALMQKPTILLADEIVSELDHVTSREIMDLIAEAQKRMNLTAIMVHHDIQLALKYANRVAVIKKGQKILEIGVEGDTIVDFQTGDMNTEEIMEMYANDTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS35</accession>
    <name>A0A2S2KS35_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_12730</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866886.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS35"/>
    <dbReference type="GeneID" id="76209228"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="142" mass="16716" checksum="5EA645741A5408CE" modified="2018-07-18" version="1">MPEIREKQHDDFEVFDKINQRYFSEIEHSVPHFQQTLFDLQNECYKLWKNTINANVQLQKELLGKSGFNFVLPKITQKIIDNVGAEIVKYRLACIKIAITTIESGTKNVKTWNDSANTFLDLNRKIIHSWFLPFMPKPDTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS37</accession>
    <name>A0A2S2KS37_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_12540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877094.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS37"/>
    <dbReference type="GeneID" id="76209250"/>
    <dbReference type="OrthoDB" id="2965at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="80" mass="8473" checksum="65FDB6D430EE41E9" modified="2018-07-18" version="1">MSNAMIYVGIAIGVVAMAGAIAYGTANQQNYISEDAVLAPAAEQGLLPKTGVLGADLNKEKWHEDPFGDLAQKIREQNGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS40</accession>
    <name>A0A2S2KS40_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ArsR family transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS40"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="34560" checksum="F8179D07FCF1530F" modified="2018-07-18" version="1">MKISLYTIPACKNTMGKGYSTESIREKLISSLEDSVTGMSGVELSKKVGVNRITMSKYLKVFAAEGLLRQKNIGNVTLWFLEPGQESFNFPDDYFKISPIFLDYLVKGTDEQTHSLIRNCLHSGATTDRLILEVIFPAIDYVQNLYDAGKIGTAELSLLKTTISKSFQIFNQIPIVSDPKKNVVVIAADSQSILISEAASAAYHSDSWKVSHLGDMSFAIDVLFDLDFQKLIGKIWKQKPGMLLVVVFSNSDEGLNFFANSINPIKDKSGKHMKLALCGKISKKSKINSDLVSEKLDDILQWSKTVSQNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS80</accession>
    <name>A0A2S2KS80_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Blue (type 1) copper domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_12940</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS80"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000923">
        <property type="entry name" value="BlueCu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052721">
        <property type="entry name" value="ET_Amicyanin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36507:SF1">
        <property type="entry name" value="BLL1555 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00127">
        <property type="entry name" value="Copper-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Blue (type 1) copper" evidence="4">
        <location>
            <begin position="46"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="261" mass="28165" checksum="48D0EFD85B1C673F" modified="2018-07-18" version="1">MFVLVLGSSTLAFAASTYEIKIPSGASDPGAPFFWSEKSTGVTTGVITIYPGDSVTWKNADTAFHTITSVTQSGEENGLFDSGLFTAGDSYTKEFTDLGDFYYYCSLHPWMNGVVHVVKNPGSVQSVSRVASGYSDDGLGFEVKYILDTNLANAVHVDPNGRSLTFTIAGDTENEQITIILPPTLIENPDTVWVDGDLAEIQIEETATGSKLIIPIKPHSKEIKVMGSYVIPEFGILTMAILSIGVFSTLFVVRSRLSIAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS97</accession>
    <name>A0A2S2KS97_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rps11</name>
        <name evidence="9" type="ORF">NZNM25_11190</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Located on the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876964.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS97"/>
    <dbReference type="GeneID" id="76208538"/>
    <dbReference type="OrthoDB" id="12054at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044391">
        <property type="term" value="C:ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.80:FF:000018">
        <property type="entry name" value="40S ribosomal protein S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="entry name" value="Ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_uS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019961">
        <property type="entry name" value="Ribosomal_uS11_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_uS11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_uS11_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03628">
        <property type="entry name" value="arch_S11P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007176">
        <property type="entry name" value="PRK09607.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="166" mass="17722" checksum="0B54B10375AE89D9" modified="2018-07-18" version="1">MSEIEAQVEEVSVEEAEIPVEEVETDVETKAQPEAKKEGPDKWGIAHIYSSYNNTIIHMTDLTGAETVAISSGGIHVNADRYESSPFAAMKAANAVVESARTKGFTGFHIRVRAVGGVGSRVPGPGAQAAIRALARGGFKIGRIDDVTPIPHDTTRKKGGKRGRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSF4</accession>
    <name>A0A2S2KSF4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Transcription initiation factor IIB</fullName>
            <shortName evidence="7">TFIIB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tfb_6</name>
        <name evidence="7" type="synonym">tfb</name>
        <name evidence="9" type="ORF">NZNM25_13670</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the TFIIB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877180.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSF4"/>
    <dbReference type="OrthoDB" id="7429at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20550">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000023">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00383">
        <property type="entry name" value="TF2B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023484">
        <property type="entry name" value="TFIIB_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="repeat" description="1" evidence="7">
        <location>
            <begin position="123"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="8">
        <location>
            <begin position="123"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="2" evidence="7">
        <location>
            <begin position="217"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="8">
        <location>
            <begin position="217"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="306" mass="33758" checksum="E1C8363DEF3E2AE2" modified="2018-07-18" version="1">MVTANQNSLCLRCGKKSMLSDDVTGERFCGKCGFVISETLQDSGPEWRSFSKDGGTDPTRTGAPSSLMIHDMGLSTVINPINKDASGKPLSTSMKSTIERLRTWDSRSQVHEPIDRNLRQALSDLNKLKDKVAIPANVLEKASYIYRKALEKKLVRGRSIAAMIAAALYAACRDTETPRTLKDIADAANVKRKDIARCYRLLHHELELKMPVVDSIQCIARISSKLDISEKTKRYAVKVLKDAQERKESAGKDPMGLAATALYLSCVHNGVSITQRDLAEAAGVTEVTIRNRYKGLKADQTTKSEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSG8</accession>
    <name>A0A2S2KSG8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_14210</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146196002.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSG8"/>
    <dbReference type="GeneID" id="76209074"/>
    <dbReference type="OrthoDB" id="3270at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="235" mass="25887" checksum="DC4626A29B7DB102" modified="2018-07-18" version="1">MNSKTKYMIPAFAAVFALMFTVALPSVMAEGGDYAKWSGEKDHKKGHHGNKAIQVEGFTGSIAIPAEMTKETHSELKNQVTVSLGQAVSVAESNGVTDAMKASIGIAKDGEGNKYLVWTITSMNKDESETMTKNIFVVDAGDSTNFTTVTKTFDHSKMKEMKGEKTVKKFEKFQQKFSEPTGDADVDAARAQFLDLMQQLRDAYNNGDSDAVKTIHDQLKELKQKVFLNMKSSHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSH8</accession>
    <name>A0A2S2KSH8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cation transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_14310</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877237.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSH8"/>
    <dbReference type="GeneID" id="76209064"/>
    <dbReference type="OrthoDB" id="290964at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006829">
        <property type="term" value="P:zinc ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="entry name" value="Cation efflux protein, cytoplasmic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040177">
        <property type="entry name" value="SLC30A9"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13414">
        <property type="entry name" value="HUEL-CATION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13414:SF9">
        <property type="entry name" value="PROTON-COUPLED ZINC ANTIPORTER SLC30A9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="Cation efflux protein cytoplasmic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="7">
        <location>
            <begin position="10"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="309" mass="33704" checksum="71A68F3109289DDF" modified="2018-07-18" version="1">MASGSTRAVYAALFGNLGIAVTKFIAAAMTGSASMWAESYHSTSDTVNQILLLLGIKISKKPATDLHQFGFGKSQFFWSFIVATMIFGISGILSLEQGFSSLLGTGHHFENPVINYIVLAIAFGFEANALRIALIQFKKPIKERGESILPSTLYNEFKNSKDTSILTVVVEDTAALLGIGIAAVGIFLTDITENTVYDSISSIIIGILLMSFAFFLAKENRGLLIGESISPEQRKQIVDIVTAIPEVNKVVTIKTMHLSPTVIIVGIEVNLIDGLDTDKIEIITDVIEHKIMKILPNSNKEYVFVEIER</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSJ0</accession>
    <name>A0A2S2KSJ0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription initiation factor IIB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tfb_7</name>
        <name evidence="9" type="ORF">NZNM25_13750</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TFIIB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_264953968.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSJ0"/>
    <dbReference type="GeneID" id="76209124"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20550">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000023">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Cyclin-like" evidence="8">
        <location>
            <begin position="106"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="8">
        <location>
            <begin position="200"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="287" mass="31600" checksum="2DE1C555CCC8E653" modified="2018-07-18" version="1">MLSDDVTGERFCGKCGFVISETLQDSGPEWRSFSKDGGTDPTRTGAPTSLAMHDRGLATIISPMNKDASGKPLSTSMKSTIERLRTWDSRSKVNASADKNLRQALSQLSTLKDKLSLSDSVIEKASYIYRKALEKGLVRGRSISALIAAALYAACRDTETPRTLKDVADAGNIKKKDISRCYRILHQELELKMPVVNPVQCIARISSKLDISEKTKRYAAKVLQIARDHEESAGKDPMGLAAAALYLSCVKNGEDRTQRDIAEASNVTEVTIRNRYKGLRLDQDTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSJ2</accession>
    <name>A0A2S2KSJ2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_14230</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSJ2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="240" mass="27725" checksum="F5BA7EC023234650" modified="2018-07-18" version="1">MLERVILTNDYDVKTVVIRKNLEEEDALKLVEQKKTDPFKSLLSRPKKEDIHIDSLKLCYESSLIVSGKYTANYFRKAIHSIDVDSNVHGVVLGDAIFPVRSKSGLEKAFVGKKGKNKVDLSLEEHVFVEEEDELTFDHHGLEIELPFKIDSKTIENYPKKILEGGSSVIRKPEFSYDSVIDKLQFHLKKPMEEDVRDLKDEFVLHDITEVYIPIFEARLSDLKKKVEIIRIDAVRKKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSJ5</accession>
    <name>A0A2S2KSJ5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_14040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877216.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSJ5"/>
    <dbReference type="GeneID" id="76209095"/>
    <dbReference type="OrthoDB" id="7466at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46438">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46438:SF11">
        <property type="entry name" value="LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="24"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="263" mass="29270" checksum="13C14A951F35784C" modified="2018-07-18" version="1">MEEKFLQIDENKIRYLESGDSQKTLVLVHGLGASAERWNKVIPIFAKNYRVLVPDLIGFGYSDKPLVDYTPDFFSDFLKKFFNATHIDRASVVGSSLGGQISAEFTISNPHNVEKLVLTSPSGVMEHSTPALDAYIMAALYPNEINAKKAFELMEGSGEEVPEDVVHDFVERMKLPNAKLAFMSTILGLKNSKLPASKLESITNPTLLIWGVDDPVIPIENSDAFVSKIQGCRLFKIDKCGHTPYVQKPNIFSSKVLEFLDDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSK5</accession>
    <name>A0A2S2KSK5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_14140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829088.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSK5"/>
    <dbReference type="GeneID" id="76209081"/>
    <dbReference type="OrthoDB" id="4990at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="123" mass="14557" checksum="8FD0BEAE89A7B56F" modified="2018-07-18" version="1">MSDFQLKYLADKVHVHRWPKDSPDWDESTIVQLDKTINKNPEKKQIVVGESIIQIQTIKFYSIKKVGVTVPLFKDECTMIFEAKFGELFAHVHVTIKNENYLDIFNQLISWRKKYFPSNEDNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSM8</accession>
    <name>A0A2S2KSM8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_12490</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866882.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSM8"/>
    <dbReference type="GeneID" id="76209255"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF34">
        <property type="entry name" value="REGULATOR OF TELOMERE ELONGATION HELICASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="5"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="561" mass="64759" checksum="75EAE075E9740EBD" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSP6</accession>
    <name>A0A2S2KSP6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_14830</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase F subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877281.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSP6"/>
    <dbReference type="GeneID" id="76209012"/>
    <dbReference type="OrthoDB" id="24971at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10580">
        <property type="entry name" value="ATPase, V1 complex, subunit F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008218">
        <property type="entry name" value="ATPase_V1-cplx_f_g_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036906">
        <property type="entry name" value="ATPase_V1_fsu_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01990">
        <property type="entry name" value="ATP-synt_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159468">
        <property type="entry name" value="AtpF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="97" mass="10513" checksum="9D88306F22A7122A" modified="2018-07-18" version="1">MKIFTVGSKSFVTSFQLAGVPGIISETPQKALDEIKRLTDDSDVGLVLVSDDITEPINDELTALRAEKSTLVFALPATGSEKTEVDYRVMLKKILGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSP7</accession>
    <name>A0A2S2KSP7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-isopropylmalate synthase</fullName>
            <ecNumber evidence="4">2.3.3.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Alpha-isopropylmalate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_14770</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-isopropylmalate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21524"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the alpha-IPM synthase/homocitrate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.13" evidence="4"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877276.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSP7"/>
    <dbReference type="GeneID" id="76209018"/>
    <dbReference type="OrthoDB" id="6555at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07940">
        <property type="entry name" value="DRE_TIM_IPMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.260:FF:000001">
        <property type="entry name" value="2-isopropylmalate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000010">
        <property type="entry name" value="2-isopropylmalate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.270:FF:000003">
        <property type="entry name" value="2-isopropylmalate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050073">
        <property type="entry name" value="2-IPM_HCS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013709">
        <property type="entry name" value="2-isopropylmalate_synth_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011830">
        <property type="entry name" value="LEU1_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054691">
        <property type="entry name" value="LeuA/HCS_post-cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02090">
        <property type="entry name" value="LEU1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002085">
        <property type="entry name" value="PRK00915.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002086">
        <property type="entry name" value="PRK00915.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277:SF9">
        <property type="entry name" value="2-ISOPROPYLMALATE SYNTHASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277">
        <property type="entry name" value="HOMOCITRATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22617">
        <property type="entry name" value="HCS_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08502">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00917">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110921">
        <property type="entry name" value="2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="11">
        <location>
            <begin position="3"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH34686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="504" mass="54229" checksum="BF97EBC636072309" modified="2018-07-18" version="1">MKVRIFDTTLRDGEQTIGVSLSPDQKLSIAKKLDELGVDAIEAGFPVISDGELKAVKMITSEGLSCEIAGLTRTIKNDIDAAVDAGLNYIHTFIATSDIHLEYKLKMTRDQALEKAIEAVEYGKSLGLQVEFSAEDATRTDREFLKKVFGEVAKAGADRVNIPDTVGYSTPEYIAEITRDTVEATHLPVSVHCHNDFGLAVANSLSGIHAGASCAHVTINGIGERAGNASLEEFSMALKCLPYEQKYETNIKSELIYDTSRFISKTVGIMVQPNKAIVGANAFGHESGIHTHGVLSNPLTYEPISPELVGRKRWLQVGKHAGVHGMNAMLAEYGVKPTEEQSKQILDKVKILGDAGKQITDVELLSIASDVLGEKELKRIVQLTGFSVSTGIGTMPYAFVKLNIDGQDHIGTDYGVGPVDAALNAIQKITGKISEIRIKDYGLASISGGSSALCEVTVKVEDALGNKVSAKSVGEDIVTTSVKAVIDAINRIMLKKMLQEKQVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSR4</accession>
    <name>A0A2S2KSR4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_15110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSR4"/>
    <dbReference type="OrthoDB" id="4140at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="80" mass="9501" checksum="78DD3CD285B60B9E" modified="2018-07-18" version="1">MYKISVHLGTCIATQQNKPTSYWTVLNHYGITHTDLKKLNLSYEELYEIYKIIQRHEEIREFLKNLKEKIAMTAKNNNVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSS8</accession>
    <name>A0A2S2KSS8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bacterial repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_15070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="500"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1004"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1267"/>
            <end position="1329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1389" mass="160588" checksum="DDE6B8D5F90BF568" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSU0</accession>
    <name>A0A2S2KSU0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">PAS domain S-box protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_14120</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_160049223.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSU0"/>
    <dbReference type="GeneID" id="76209086"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00075">
        <property type="entry name" value="HATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065:SF10">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065">
        <property type="entry name" value="SENSOR HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="PAS" evidence="8">
        <location>
            <begin position="29"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="9">
        <location>
            <begin position="83"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="175"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="374" mass="42202" checksum="A86E4BD816FA3B52" modified="2018-07-18" version="1">MGLTSSLKKLNLNLSDLEAILADHSITTVTDNEGIIVYANKKFCEISKYSEEELLGQNHRLLKSGEHSDQFYNKLWTTISGGRNWDGEIKNRAKDGSFYWVKTTIIPVMDSQKNIKYYVAIRTDITREKQMQEKLLHAEKQLDQQNLSLAAKVAMKSSDIVKNERLATIGTMASRIAHDLKNPLTILHTYADMLTPEILSKLDSKDKEKWFRLQNSIFDMHRIIDDVLDFARTSEINKKKVSFLSILRLSMNHVRSSYGIIIKLPENDVTLNCDRRKIEGVMSNLINNAVHALDGQGEIDISLSLDSEFVTIKVKDSGPGIPDENMEKIFEPMFTTKNTGTGLGLVICKSIVEQHGGNISVSNNPTTFTIKLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSW2</accession>
    <name>A0A2S2KSW2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA-guanine(15) transglycosylase</fullName>
            <ecNumber evidence="6">2.4.2.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">7-cyano-7-deazaguanine tRNA-ribosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Archaeal tRNA-guanine transglycosylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tgtA</name>
        <name evidence="8" type="ORF">NZNM25_15300</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Exchanges the guanine residue with 7-cyano-7-deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D-loop) of archaeal tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>guanosine(15) in tRNA + 7-cyano-7-carbaguanine = 7-cyano-7-carbaguanosine(15) in tRNA + guanine</text>
            <dbReference type="Rhea" id="RHEA:43164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10371"/>
            <dbReference type="Rhea" id="RHEA-COMP:10372"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:82850"/>
            <dbReference type="EC" id="2.4.2.48"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">tRNA modification; archaeosine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeosine tRNA-ribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.48" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877367.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSW2"/>
    <dbReference type="GeneID" id="76208459"/>
    <dbReference type="OrthoDB" id="6871at2157"/>
    <dbReference type="UniPathway" id="UPA00393"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002099">
        <property type="term" value="P:tRNA wobble guanine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="entry name" value="Queuine tRNA-ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10630">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01634">
        <property type="entry name" value="TgtA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050076">
        <property type="entry name" value="ArchSynthase1/Queuine_TRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004804">
        <property type="entry name" value="TgtA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00432">
        <property type="entry name" value="arcsn_tRNA_tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46499">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46499:SF1">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88802">
        <property type="entry name" value="Pre-PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="tRNA-guanine transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA-guanine(15) transglycosylase-like" evidence="7">
        <location>
            <begin position="11"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="503" mass="57587" checksum="3B1F4261FF9DBB5F" modified="2018-07-18" version="1">MFEISKTDLAGRIGTLYTNHGKIETPAYVPVIHPVKQTIPSKKIRDIGFDLVITNAYITRNNYGDDAIKKGIHKVIDFDGAIMTDSGGYQVLEYGDVKVSPPEMANFEKGILTDFAIPLDKPTGFGMPIKKAEAYVKHTLQVSKQTLENSEDNGQIWIGPIQGGEHFDLVAKSTKSLVKIGFQMLALGSPVEFMESYEYRLLAQMIVAAKKQMPHSIPLHLFGAGHPLTIPFAIALGCDTFDSASYMLYAKQSRYITDDGTRYLSDITVFPCNCEICSKYSPEELRNLDEVDRINELAIHNLYAIKLEVDKVKQAIHEGRLWEYVIKKARAHPKLFEMIEVMTENYKFLGLSTPKFKEKAIFLYSKEDQYRPEVQSYHNMVRKFKSKKKKLLITKESSTKPGYLSNQYVGLKRKFKDFESMQICQYNPILGLIPIEISDIFPAAHHETVRINFEPKEFPAFEKTWNDFFSNNKFLEIHYDKNDGFLKHFVKTLPKEIKRKSIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSY0</accession>
    <name>A0A2S2KSY0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13490</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877166.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSY0"/>
    <dbReference type="GeneID" id="76209150"/>
    <dbReference type="OrthoDB" id="2524at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="62" mass="7288" checksum="D92684F3FB7EE7A6" modified="2018-07-18" version="1">MASYSSQVNVIHKKFQNAVKRAKTKQALNKAYSAHKKDHERLLKKHLREETLMIIKAKKKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT00</accession>
    <name>A0A2S2KT00_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 18">Inosine-5'-monophosphate dehydrogenase</fullName>
            <shortName evidence="13">IMP dehydrogenase</shortName>
            <shortName evidence="13">IMPD</shortName>
            <shortName evidence="13">IMPDH</shortName>
            <ecNumber evidence="13 18">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13 20" type="primary">guaB</name>
        <name evidence="20" type="ORF">NZNM25_15640</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13 18">
            <text>IMP + NAD(+) + H2O = XMP + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="13">Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13 18">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 13">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13 17">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.205" evidence="13 18"/>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877394.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT00"/>
    <dbReference type="GeneID" id="76208423"/>
    <dbReference type="OrthoDB" id="21361at2157"/>
    <dbReference type="UniPathway" id="UPA00601">
        <property type="reaction ID" value="UER00295"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006177">
        <property type="term" value="P:GMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04601">
        <property type="entry name" value="CBS_pair_IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00381">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000003">
        <property type="entry name" value="GMP reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01964">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005990">
        <property type="entry name" value="IMP_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015875">
        <property type="entry name" value="IMP_DH/GMP_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01302">
        <property type="entry name" value="IMP_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911:SF111">
        <property type="entry name" value="INOSINE-5'-MONOPHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911">
        <property type="entry name" value="INOSINE-5-MONOPHOSPHATE DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000130">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01240">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51412">
        <property type="entry name" value="Inosine monophosphate dehydrogenase (IMPDH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00487">
        <property type="entry name" value="IMP_DH_GMP_RED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6 13 18" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="4 13 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13 14" id="KW-0520">NAD</keyword>
    <keyword evidence="9 13 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 13 15" id="KW-0630">Potassium</keyword>
    <keyword evidence="7 13 18" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="19">
        <location>
            <begin position="92"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="19">
        <location>
            <begin position="151"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimidate intermediate" evidence="13">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="244"/>
            <end position="246"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <begin position="294"/>
            <end position="296"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13 15">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13 15">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13 15">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="334"/>
            <end position="336"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="357"/>
            <end position="358"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="381"/>
            <end position="385"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="413"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="467"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="468"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="GBH34773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="476" mass="50567" checksum="5C75EBBFEE8221BE" modified="2018-07-18" version="1">MEFKEGLTFDDVLLVPKYSDITSRSQTDLTTKLSRNISINIPFVSANMDTVTESSMAVAMARAGGIGIIHRFLTIEEQAHEVLKVKRSGSIMIENPYSITSNKSVQDAIDYANDMEISGLLVVDSNSKLIGIITERDLLFADPKLRIEDVMTKDVITAKFGVSLDESKEILHKHRIEKLPIVDDSGMIKGLITSKDITNNADFPNASKDKKGRPLVGAAVGVKGDFLERSESLLEAGADVLVVDIAHGHSENALSTIRNIKKAFPDCELIAGNIATAQGAEDLIKAGVDAVKVGVGSGSICITRVITGSGVPQLTAVMDCAKIGKDYGIPIISDGGTRTSGDATKALASGASSVMVGSMLGGTDESPGTVLTKNGKRFKVYRGMASLAASIGRKSKETGSISLDDDLNDYVAEGVEAMVPYKGTVTDILKQLTGGVRSGLSYCGAHTITQMQENAEFIKMSRAGFAESQPHDVSLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT41</accession>
    <name>A0A2S2KT41_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hemerythrin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_16050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877442.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT41"/>
    <dbReference type="GeneID" id="76208381"/>
    <dbReference type="OrthoDB" id="131831at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.520">
        <property type="entry name" value="nmb1532 protein domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012312">
        <property type="entry name" value="Hemerythrin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39966">
        <property type="entry name" value="BLL2471 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39966:SF1">
        <property type="entry name" value="HEMERYTHRIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01814">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hemerythrin-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="185" mass="21296" checksum="F323A67C38083015" modified="2018-07-18" version="1">MSTASLRRDHDLIEKVVKAMESTIQLLNDNKQIPESILLPVIDFSKNFTDVCHHTKEEKSLFPALEQAGMPSNMGPVAMMLIDHQRSREIGSRMEESAKEYLSSGDSTKLISDMQQYVEHITEHLWKENNRLFMMAEARLQYVSKKVDDELNEIEKTQLNDLGKSREHYEKLAENLSKDVSEQGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT44</accession>
    <name>A0A2S2KT44_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_16040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT44"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="91" mass="10583" checksum="2784A5D985B4989A" modified="2018-07-18" version="1">MGVRKFANGDIELDFYHEDEITEYRYSSDPSRLGNFPKELAETLANTLALDICVEIYFDEDGSPTYIELEECDDEDFDDDEDFDESSEMNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT66</accession>
    <name>A0A2S2KT66_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 14">DNA ligase</fullName>
            <ecNumber evidence="14">6.5.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Polydeoxyribonucleotide synthase [ATP]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14 17" type="primary">lig</name>
        <name evidence="17" type="ORF">NZNM25_14460</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 14 15">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="14"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877249.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT66"/>
    <dbReference type="GeneID" id="76209049"/>
    <dbReference type="OrthoDB" id="31274at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07901">
        <property type="entry name" value="Adenylation_DNA_ligase_Arch_LigB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07969">
        <property type="entry name" value="OBF_DNA_ligase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3260.10:FF:000007">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000062">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.30:FF:000012">
        <property type="entry name" value="Probable DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00407">
        <property type="entry name" value="DNA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022865">
        <property type="entry name" value="DNA_ligae_ATP-dep_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF4">
        <property type="entry name" value="DNA LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13 14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 14" id="KW-0132">Cell division</keyword>
    <keyword evidence="8 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 14" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="12 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 14" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 14 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="10 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="16">
        <location>
            <begin position="330"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="active site" description="N6-AMP-lysine intermediate" evidence="14">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="423"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GBH34655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="588" mass="66662" checksum="2E2BC2E39A29153A" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT73</accession>
    <name>A0A2S2KT73_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_16350</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT73"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="500" mass="56671" checksum="6BEE376E8A4F0C9D" modified="2018-07-18" version="1">MFGHGLGGEVLPPIIIENKQASLSISITPSVFDENPEKYISLRLFDSNTDAIIEHVTFEFELKKDGKQIFKDIFHDELGNLNIKVFTDNSNNITIEGIKEPILGGWMKSNSNPLILRGPVFTSGGLYEYTIKILSINSDSNIINEEIILDGGISLAQTNYFNVNDYLNEEKTLQVISYFDTINNFNFESNTIEFSMPFDWNQNLEQLSVVHQEIRVPNTFGDFLSTRYDSTVNGILLPDESVTIDDYSFEDRTIHLVVNQELIKQIQNEAIRTSDTTMNFQLRPSETVKFPLELVTPDLRYEVFLSWEPEIIHTEGEITFFVTFEEIFSDKSKKLVEYDLSIIQKNNEIFSKHLTGNVNSQTPNSHKITFSNEQSGTANFVLSNIGGNSLSKGNFILVIQSPSDIQNDSSIIPEWIKNNAAWWADGSIDDNSFVQGIQFLIKEGILQIPYTTQGSSSTSNQIPEWIKNNAVWWSEGTIDDDSFIQGIQFLIKEGILQITS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTA0</accession>
    <name>A0A2S2KTA0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_16650</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877490.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTA0"/>
    <dbReference type="GeneID" id="76208320"/>
    <dbReference type="OrthoDB" id="6552at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036507">
        <property type="entry name" value="Telomere_rpt-bd_fac_dimer_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63600">
        <property type="entry name" value="Telomeric repeat binding factor (TRF) dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="222" mass="25313" checksum="07C26F9A9067D52D" modified="2018-07-18" version="1">MKVKSNTKLAIFDTFKTKGDDLTGEANRQRSIIAILGSNANAAERTRTGISQRIAKKHGIAWKNIYSGIFRDLDEILLPMEIAEEDGRLPLKRGPKALQEKGIPYYHLTKKGILIALSLNEIKNREELLKEFFSKAESSEKEFEKILTSLLKISPNFTYSIFQKYVKAFCENKIEELLPFDLSKLKDISDESLTIQKEILLAFLKLSKQEKGEAIIFLDKIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTC3</accession>
    <name>A0A2S2KTC3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PEFG-CTERM sorting domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_17110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877529.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTC3"/>
    <dbReference type="GeneID" id="76208273"/>
    <dbReference type="OrthoDB" id="11500at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="298" mass="33900" checksum="893EEA3F7E78B03B" modified="2018-07-18" version="1">MNSKIFGFLVILLIISIIPTIDAQISFGEKASQKSVEIVINSVGDVHVKHVISKTDLPKQVELIYGTVSNLSVTNEKGEEKQFSVIGDNAGILIFPSDNNSIVEYDLDHAISKKDNVWTWSFRYLETTSFILPEEADLIFANERPIYLDEKKGITCHGCQMILEYSINEPKIYQNIKWEDKEFLVEIRSHSNIDGFVFDQPTKSITFDVSEKNRFVTTIIPLELLWGPYAVFLDDEKIYFNQYINNGTHVWLNIRPETTGEVTIIGTTVVPEFPIIAPLAMGFLIIVITPLIRKVNLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTF0</accession>
    <name>A0A2S2KTF0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_17410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877549.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTF0"/>
    <dbReference type="GeneID" id="76208244"/>
    <dbReference type="OrthoDB" id="10608at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052276">
        <property type="entry name" value="Diphthamide-biosynth_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44240">
        <property type="entry name" value="DNAJ DOMAIN (PROKARYOTIC HEAT SHOCK PROTEIN)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44240:SF10">
        <property type="entry name" value="J DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="4"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="383" mass="43245" checksum="F28602574CD5D8D7" modified="2018-07-18" version="1">MVENNYDILGIVEGSTEKEIRDAFRRLALQFHSDRGGENDQFIKIKQAYEDLKIGKKYPDTDIEKIKNSRVYSGDSEADIRRKNQILGQELSKEMKTAEEWASALNRSNMTGRKLFGSKTLGEIELERKANGALSIKGNFMAGSLTYDGPIIMQGSITSPSWTQEFKTNIHLTSGDFKFLDPIENKYRIDNGATIIVDNGNIVVGNVFGRKFRVEDPEGKVGVFQIQEHRTHLSAPKGKIIAENLVNTVSIDADSVIVLNVEDDVMISAREILFYGGKFTYDSLIELKNGGSIRFFENFSIQGLSGDAIIQLENGKKIRLFDLKTKKIRDLSDEYVPNKENYGKEDTMVGHGFTITYDMLNNISKKPTKKQKSWTSKFSFSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTF3</accession>
    <name>A0A2S2KTF3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_17140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTF3"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="162" mass="18712" checksum="E4A5E139695B3DA7" modified="2018-07-18" version="1">MLARIKVKIGENEIEVDSRDFYVDNQTLGEIIDSISNHLPENQAKIVYETELIPKPSEIKQDNQYGLEYLDNAEVSEPEFDEPQYITSNEIKSKLRILETSRFFDSPRTVTETVQQLREYGWSASPLDVSKALAKMASNKEIMKNSYENRTHYFVEEALVAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTF6</accession>
    <name>A0A2S2KTF6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lon N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_16520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTF6"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="1">
        <location>
            <begin position="6"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="198" mass="22493" checksum="4BE02014FF163A38" modified="2018-07-18" version="1">MIGDGQFGVCLLDENNTVKGWNSPKLVGTIAKITKCEDIELDGLQLHIETIGRNSFKIRKIIPPSISQPENYDPLSVEGHQEISDIHEKIGTEEKMYIQAEVEMIPEIDENISLEKWEKLVELWKKKIINQALPQVVEPHALDHVLEQYYLTTDTPTIDYIYSLSALGAKDPNELQPILEATTMDDLIQKVEELLTVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTF9</accession>
    <name>A0A2S2KTF9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_16380</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877467.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTF9"/>
    <dbReference type="GeneID" id="76208347"/>
    <dbReference type="OrthoDB" id="28610at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36216:SF1">
        <property type="entry name" value="HTH ARSR-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36216">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="166" mass="19091" checksum="F5C491BB9A1C6DF5" modified="2018-07-18" version="1">MTDRNSQLQQIIEENPGIQFREIMRSSGLKNGVLSHYLGKLEKSGIIKVIRGPRQARFYPPRITENESIVIKALRKETPRDLLLALIKEDGLEFNHLVKEVRKSPSTVSLYLSQIVGDGLVEIKLVQLKKRYYIKARELIDKLVEEYRPGLLEKPTSGFEDIINSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTG3</accession>
    <name>A0A2S2KTG3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetrapyrrole methylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_17240</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTG3"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11646">
        <property type="entry name" value="Precorrin_3B_C17_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006363">
        <property type="entry name" value="Cbl_synth_CobJ/CibH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051810">
        <property type="entry name" value="Precorrin_MeTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01466">
        <property type="entry name" value="cobJ_cbiH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47036">
        <property type="entry name" value="COBALT-FACTOR III C(17)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47036:SF1">
        <property type="entry name" value="COBALT-FACTOR III C(17)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="6">
        <location>
            <begin position="6"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="266" mass="29551" checksum="D650FF55883CDD60" modified="2018-07-18" version="1">MKLPGKLYIVGVGPGHHDHMTFRAKEVIEESDTIVGYETYVNLVQDLIEGKTVYRYAMTQEVERAHQCIDLAKSGKIVSLVSSGDPGIYGMAGLIYETLAESNWNPKDGLQVEIIPGVSALNSCASIIGSPLMTDFAVVSMSDLLVPWEIIEKRVEAAAQGDFVIVIYNPASKKRIHQLQDTRKILLKYRKPTTPVAIIKGAFRESETIVMTDLENLPNHSDKLGMISTVIIGNSSTYTYKDLMINPRGYKSKYNLEEQTNPDLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTG4</accession>
    <name>A0A2S2KTG4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Peptidase S8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_15390</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTG4"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF11">
        <property type="entry name" value="CEREVISIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1242"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="7">
        <location>
            <begin position="152"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="1270" mass="139956" checksum="7400A273E8AE773D" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTI0</accession>
    <name>A0A2S2KTI0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">A-type ATP synthase subunit B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">atpB</name>
        <name evidence="12" type="ORF">NZNM25_16580</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane. The B chain is a regulatory subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877483.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTI0"/>
    <dbReference type="GeneID" id="76208327"/>
    <dbReference type="OrthoDB" id="32941at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18112">
        <property type="entry name" value="ATP-synt_V_A-type_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18118">
        <property type="entry name" value="ATP-synt_V_A-type_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01135">
        <property type="entry name" value="V_A-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00310">
        <property type="entry name" value="ATP_synth_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022879">
        <property type="entry name" value="V-ATPase_su_B/beta"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003235">
        <property type="entry name" value="PRK04196.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389:SF4">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039114">
        <property type="entry name" value="V-ATPsynth_beta/V-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 8" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="5 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="10">
        <location>
            <begin position="12"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="9">
        <location>
            <begin position="135"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase A/B type C-terminal" evidence="11">
        <location>
            <begin position="358"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF22919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH34867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="461" mass="50684" checksum="1C2E9DA54FC2A2CA" modified="2018-07-18" version="1">MTTKGGVQYSKIAEIKGPLVVVDDVENAAFDELVEIETTDGERRLGKVLEVGNGKAIVQVFEGTTGLSISGTNAKFVGKVMEMPVSKEVLGRVFDGLGRPKDGLPDPIADKFIDINGEPMNPEQREYPKDFIQTGVSVIDGLMTLVRGQKLPIFSGSGMSHNLLAAQIARQASVIGTSDDFAVVFAAIGVQYSEAEYFRRSLEESGALKRSVLFLNTADDPAIERIITPRVALTVAEYLAFELGMHVLVVITDMTNYAEALREISAAREEVPGRKGYPGYLYTDLSTIYERAGKLDGKKGSVTQVPILSMPSDDITHPIPDLTGYITEGQIVVGRDLFRQGVYPPINILMSLSRLMKDGIGEGSTRDDHSEVANQVYDAYSRAQEVRALAGIVGKAGLTEIDLKYMDVGDTFEKEFLTQATDENRTIEETLALMWKIVSKLPKNEITKIKDKYVEQYYKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTJ2</accession>
    <name>A0A2S2KTJ2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_17530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTJ2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="231" mass="24999" checksum="09C2E645F2AF316B" modified="2018-07-18" version="1">MIFSNEIGVLFPTTSASVIDSLKNDVSNFGSKASASVEQRIDGSVDKIIDKTGNTISNEITQTGEKISNEISDVKESSQKILKEEISNFNPIESVKKIFSTNSTSQNSKSIGSSVSSPPINPTTVQTNSPITYETLSLSTKQQSDDNILLHYSDSSGKTNSVNVIIRTAEKEIFSGTYFTSMFETTLNDASGIPYFVDMIIDHQDYGLVSSSVFNPGDSSDSNINGVFSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTM5</accession>
    <name>A0A2S2KTM5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_16060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877443.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTM5"/>
    <dbReference type="GeneID" id="76208380"/>
    <dbReference type="OrthoDB" id="3312at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="85" mass="9341" checksum="E2CE6A4813431CA9" modified="2018-07-18" version="1">MAVKKKATTKRKTTTKKKAAPKKKAAPKKKATKSNTKKPAFGGYAISFAGRKETVEQVFGNKPIAPSEMTKKIWAFVKSKSLSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTT0</accession>
    <name>A0A2S2KTT0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_17580</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877565.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTT0"/>
    <dbReference type="GeneID" id="76208227"/>
    <dbReference type="OrthoDB" id="86092at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052396">
        <property type="entry name" value="Meiotic_Drive_Suppr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37171">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE YRZF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37171:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE YRZF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RIO-type" evidence="9">
        <location>
            <begin position="85"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="253" mass="28568" checksum="398130F5511B9784" modified="2018-07-18" version="1">MEHSFISIKKFSDEPYSKILGYPKATSRQIKSRINELEKLKIKSISFVGPTTIGSLSILGKGYVGVVVLAKKNNKLVALKIRRTDSQRKEMKNEADLLKLVNSVNVGPKMLESSKNFLVMEYLEGVKISDWVYLLKGVGSAKKLKSTIRKILEDCYRLDQLGFDHGELSNISKHVIVSKTKSTLIDFESSSTKRRPSNVTSITQAFFIGSGIAKKAQKIYRNSSKDQIISALKQYKQEKTRESFEKLLKTLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTU2</accession>
    <name>A0A2S2KTU2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Heat-shock protein Hsp20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_16760</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTU2"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041799">
        <property type="entry name" value="Hsp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="41"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="150" mass="17323" checksum="7751FBF701F043CA" modified="2018-07-18" version="1">MKIPRIDYKIYNQKLSIYLKMGLVKSMANEMIKEIGNKSREFYEFVLPPVDMYLDEDKLTLLVDMPGFEKKDIKLSINGNILSIQACKEIAEQGKHNMICNQRPNIIDKKIRLPIELKEGEESVNSAKYEQGILTIIVPVQKHGKDIKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTU3</accession>
    <name>A0A2S2KTU3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Dolichol-phosphate mannosyltransferase</fullName>
            <ecNumber evidence="8">2.4.1.83</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Dolichol-phosphate mannose synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Dolichyl-phosphate beta-D-mannosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Mannose-P-dolichol synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">NZNM25_18400</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25 26">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O-mannosylation of proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.83" evidence="8"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877639.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTU3"/>
    <dbReference type="GeneID" id="76210281"/>
    <dbReference type="OrthoDB" id="11098at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004582">
        <property type="term" value="F:dolichyl-phosphate beta-D-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06442">
        <property type="entry name" value="DPM1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000119">
        <property type="entry name" value="Dolichol-phosphate mannosyltransferase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039528">
        <property type="entry name" value="DPM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007267">
        <property type="entry name" value="GtrA_DPMS_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398:SF1">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04138">
        <property type="entry name" value="GtrA_DPMS_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16" id="KW-0464">Manganese</keyword>
    <keyword evidence="15 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="11 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="23">
        <location>
            <begin position="14"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="GtrA/DPMS transmembrane" evidence="24">
        <location>
            <begin position="264"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00053724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00074878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00082336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00082614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00083744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF04138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="GBH35049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="388" mass="43695" checksum="8C9EAC4B47627776" modified="2018-07-18" version="1">MALETRNANKPQVSIIIPTYNESQNILNVLKSIGDIIPKNIFTEAIVVDDNSPDGTGKIVEDYLKNVKKIAGYTVNVIHRTAKNGLSSAILSGIQRATGDTIVVMDSDLSHPPQIIPKMIETLRHYQCDMVVASRYITGGKINGWNKKRKLLSKIATMIAKKGLGVKTNDPMSGFFAFRKGILKGLNFDAIGYKILLEILVKKRGLDIKEIPYTFQNRTLGSSKLDFPIIIDYVKSVWKLYRSGKPEENNEKRKSVKFFSKAGRFYTIGATGFAINYIISLMFSGGLTEIWYLHANILGIITSMTSNFVLNKWWTFGDRDFSFKKTISQFSKFIMFSSFGALIQLGVVFSLVDNYDWMYTTALGVGVLTGAFSNFILNKKWTFKDKLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTV4</accession>
    <name>A0A2S2KTV4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MEMO1 family protein NZNM25_18910</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_18910</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the MEMO1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877713.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTV4"/>
    <dbReference type="GeneID" id="76210118"/>
    <dbReference type="OrthoDB" id="372162at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07361">
        <property type="entry name" value="MEMO_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.830.10">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00055">
        <property type="entry name" value="MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002737">
        <property type="entry name" value="MEMO1_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04336">
        <property type="entry name" value="AmmeMemoSam_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001987">
        <property type="entry name" value="PRK00782.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060">
        <property type="entry name" value="PROTEIN MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060:SF0">
        <property type="entry name" value="PROTEIN MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01875">
        <property type="entry name" value="Memo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="276" mass="30274" checksum="8E36B27607F020E9" modified="2018-07-18" version="1">MIREPVVAGQFYPDTKKELEKMIEYCIEHKFGPGKQIKESAEKIFGIVCPHAGYVYSGPTACHSYKAISSQKPELAIIIGPNHFGIGKNAATMIDAKWETPLGLIEVDSQASQEAVKNSNVIEIDNYSHSQDHSLEVQVPMLQSILTNEFKILPIILLEQNLQTATDVGNVVSEIAKKRNTIIVASSDFTHYEENSFAHRQDKALIEPILDLDVDRFYQVLMEKRVSACGYGAIASAMIACKNLGATRGELLSYATSGDVSGDTDSVVGYGAIKFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTW3</accession>
    <name>A0A2S2KTW3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_18570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877681.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTW3"/>
    <dbReference type="GeneID" id="76210084"/>
    <dbReference type="OrthoDB" id="1388at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="68" mass="8143" checksum="B3D11310AD51A3DD" modified="2018-07-18" version="1">MQIATFQPDELQSKPKSERQITYFCRLCRQYKIKTRKAHLQNFHNANRDTVTKRSNNDLVDVIFIESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU01</accession>
    <name>A0A2S2KU01_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Heme transporter CcmC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_19140</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877728.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU01"/>
    <dbReference type="GeneID" id="76210143"/>
    <dbReference type="OrthoDB" id="10156at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036257">
        <property type="entry name" value="Cyt_c_oxidase_su2_TM_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81464">
        <property type="entry name" value="Cytochrome c oxidase subunit II-like, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH35123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="86" mass="9739" checksum="528EC577A8306899" modified="2018-07-18" version="1">MKKLVGLFLVLAVIVVIPAIESAFAAGDEPGEYLDRRAIIWNLFFRMMTIAFVVGAVVSGTIIWQVWRFRESHPKAKPTPYEGTDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU64</accession>
    <name>A0A2S2KU64_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_19850</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877792.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU64"/>
    <dbReference type="GeneID" id="76210215"/>
    <dbReference type="OrthoDB" id="11395at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021027">
        <property type="entry name" value="Transposase_put_HTH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12323">
        <property type="entry name" value="HTH_OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0815">Transposition</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Transposase putative helix-turn-helix" evidence="9">
        <location>
            <begin position="3"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="7">
        <location>
            <begin position="167"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="8">
        <location>
            <begin position="294"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH35194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="416" mass="48617" checksum="89AEA8019DA7E7C3" modified="2018-07-18" version="1">MPIRNYKFRLYPNSNQEYKLQNNLAVCRWLYNKFVEQTQKSFLSRNDMNYILTELKQSEPWLYNYHSKMLQMVSTQIEGSEKALIELSKKGHKIGQLKFARYNEFKTFTYNQSGYKIETHGDKTLLYLSKIGYVEIRKHREFPDNTNIKQVIITKSKSGKWHVCITVDVEESLIYIPKISFAKAVGIDVGIKSFTYDSDGYSTPNPLNLKKMLKPLARVQRKVSRRKKGSNNRLKAILRMQKIHEKIVNQRKDFQHKLSAQYAKNNDVVFVEKLEKLNMVKNHRLARSIMDSSWGLFLQKLEYKCKLLVEVSSKNTTVDCSRCGNKVPKSLAVRIHRCNKCNLILNRDYNASINILQKGLKIFKQDKLPQELRKVTPAEILMRSRKQEEVTQFLCGSSPSFLKDSIGICLKYACRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU90</accession>
    <name>A0A2S2KU90_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ArnR1-like winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_18090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU90"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038723">
        <property type="entry name" value="ArnR1-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14947">
        <property type="entry name" value="HTH_45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ArnR1-like winged helix-turn-helix" evidence="1">
        <location>
            <begin position="3"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="83" mass="9337" checksum="FCC208122D515377" modified="2018-07-18" version="1">MGDILDVTADGGRSGIIVSAISRKANLSHYAVLDKCEKLVEAGLVESVKNDRNRVFLITEKGLQFFQEFKRFQGLVESMNLRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU96</accession>
    <name>A0A2S2KU96_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cobalt-precorrin-8 methylmutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cbiC</name>
        <name evidence="6" type="ORF">NZNM25_20150</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CobH/CbiC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877824.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU96"/>
    <dbReference type="GeneID" id="76210249"/>
    <dbReference type="OrthoDB" id="24491at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016993">
        <property type="term" value="F:precorrin-8X methylmutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10230">
        <property type="entry name" value="Cobalamin biosynthesis CobH/CbiC, precorrin-8X methylmutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003722">
        <property type="entry name" value="Cbl_synth_CobH/CbiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036588">
        <property type="entry name" value="CobH/CbiC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43588">
        <property type="entry name" value="COBALT-PRECORRIN-8 METHYLMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43588:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-8 METHYLMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02570">
        <property type="entry name" value="CbiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63965">
        <property type="entry name" value="Precorrin-8X methylmutase CbiC/CobH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cobalamin biosynthesis precorrin-8X methylmutase CobH/CbiC" evidence="5">
        <location>
            <begin position="9"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH35224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="212" mass="22866" checksum="77E4643FE5AE4CA8" modified="2018-07-18" version="1">MQTKKGQSIEDASMQMIEDEIGVHQYNEKEWPIVRRIIHSTADFDFADKNKIIFHKDAIQSGMNALRNGCSIVVDVNGVIGGLNKQNPKDFGNNIVCNISSPEIMELAKKEGKTRSQVSMRAAISDIEGGVVAIGNAPTALLEVIQMVKEGIVKPALIIGIPVGFICAAESKEELSKLEIPFITNIGRKGGSSSASAIINAIFKLIRAESSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUB4</accession>
    <name>A0A2S2KUB4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Polysaccharide biosynthesis protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_19380</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877750.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUB4"/>
    <dbReference type="GeneID" id="76210168"/>
    <dbReference type="OrthoDB" id="12322at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050833">
        <property type="entry name" value="Poly_Biosynth_Transport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30250:SF28">
        <property type="entry name" value="POLYSACCHARIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30250">
        <property type="entry name" value="PST FAMILY PREDICTED COLANIC ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="291"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="346"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH35147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="376" mass="41642" checksum="DF9DB6CB99803B34" modified="2018-07-18" version="1">MKLIRNFGSMVAGDTISGSIGSVFWLFLATLLTVSDYGQIQFLISIAALAAGFSMIANSNTITVFEVKKKDLTGILFLISLGIGGIVSLVLVIIYSRIDIIFLTFGIIVGEMVIGYFLGKKLFYKYATFLILQKILMISLALGLYFFMGLDGIIYGISLSYIPLVIIVLKNIKESEFDFKLLKKNSGFIINNYGTRLVVYSKRNLDKIIIAPILGFQVLGEFALGFQVYLVMMLFSSISFKFLLLKDSEGYNSKKFKMFVFTISVLISILGVFIAPIVIPTLFPNFIDSVEIIQILSLAVIPNTIVLFYSSSFLGNENSRVVLTGVIMSTVVYLSLIVFLSQTLGLQGIGISFVLSSIIYAIFLSIIHKKTKNLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUQ8</accession>
    <name>A0A2S2KUQ8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Single-stranded DNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_20060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUQ8"/>
    <dbReference type="OrthoDB" id="6262at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
        <property type="term" value="P:response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04491">
        <property type="entry name" value="SoSSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051231">
        <property type="entry name" value="SOSS-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356">
        <property type="entry name" value="OB FOLD NUCLEIC ACID BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356:SF0">
        <property type="entry name" value="SOSS COMPLEX SUBUNIT B HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="465" mass="51014" checksum="23E22CC5F2B29DC8" modified="2018-07-18" version="1">MSELENLMSKLLELKPELTKEDVEEQIKLKKEKIGAGYLTDQGALFLIASDFGVTLAEPLKVEMSLKDLYAGAKEISLETRVLNLSPAKQFSRKDGSPFYLRTMTVYDGNSTASVKLWDEKANLPGVENLKPGDLIKIIKAYVKSDFDGSPTINIGSGSNIETADRESEIPTIESITKDVSELQEGQKDLVISGMIDGVISGMEFTNSRGQPGKALRMRLKGKDGSGMRVVLWGKDESSIPNMISQSAKVRLLGVKVKSGNQGLEIHGNDATIIEIEGGKEAEPVIARILSMSTSENGKNMILAVDNKKNLYNISDFSNSTSICAEGDVIECMPSKIYGNSITLDDNSFVRKLDNDESIPSLSQIRTKINDVKVDGNYCIEAIILKVPERREVQTKTGESISLSEMFVEDDTGQIWVKGWRNQARLIDKCELGEIVSITGLNAKAGLEGRIELFVTAFSKITKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNQ4</accession>
    <name>A0A2S2KNQ4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl14</name>
        <name evidence="6" type="ORF">NZNM25_00300</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L24e, part of which may contact the 16S rRNA in 2 intersubunit bridges.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875912.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNQ4"/>
    <dbReference type="GeneID" id="56067640"/>
    <dbReference type="OrthoDB" id="23569at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00337">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.150.20:FF:000007">
        <property type="entry name" value="50S ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_uL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_uL14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_uL14_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006344">
        <property type="entry name" value="PRK08571.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L14/L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="140" mass="15069" checksum="F4BF0EC3A6137086" modified="2018-07-18" version="1">MAKQAGKGVEEFRPYVTRSIPVGANIVCADNSGAKILEIINVPRHKTRVSRLPAAAVGDFCNVVVKKGPAELRKQVYGAVIVRQKYAVRRLNGVRVCFEDNAAVLITPEGETKGTDIKGPVAAEASEKWPRVANLASMVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNR3</accession>
    <name>A0A2S2KNR3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00840</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNR3"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="149" mass="18318" checksum="8021FE4A46155A58" modified="2018-07-18" version="1">MSDSDEQYLQLKEIYDEQRWNLEKEFEEKFQESRKYFDEQKQAIHDKNESDSPLTPEQTDQMLKDIFFEFIERQEEIKIEYTSRVDALNAMFKIKFEQFGNEMPLWVEKVMELWQKGKISDVEFVNFLSFVINNDIIKLEQWIFSEYNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNU7</accession>
    <name>A0A2S2KNU7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1,4-dihydroxy-6-naphtoate synthase</fullName>
            <ecNumber evidence="3">4.1.99.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Menaquinone biosynthetic enzyme MqnD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">mqnD</name>
        <name evidence="4" type="ORF">NZNM25_00900</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the conversion of cyclic dehypoxanthine futalosine (cyclic DHFL) into 1,4-dihydroxy-6-naphthoate, a step in the biosynthesis of menaquinone (MK, vitamin K2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>cyclic dehypoxanthinylfutalosinate = 1,4-dihydroxy-6-naphthoate + dihydroxyacetone</text>
            <dbReference type="Rhea" id="RHEA:33087"/>
            <dbReference type="ChEBI" id="CHEBI:16016"/>
            <dbReference type="ChEBI" id="CHEBI:64254"/>
            <dbReference type="ChEBI" id="CHEBI:64270"/>
            <dbReference type="EC" id="4.1.99.29"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Quinol/quinone metabolism; menaquinone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MqnA/MqnD family. MqnD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.29" evidence="3"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875966.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNU7"/>
    <dbReference type="GeneID" id="76209418"/>
    <dbReference type="OrthoDB" id="49741at2157"/>
    <dbReference type="UniPathway" id="UPA00079"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016830">
        <property type="term" value="F:carbon-carbon lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13636">
        <property type="entry name" value="PBP2_Af1704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00996">
        <property type="entry name" value="MqnD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003773">
        <property type="entry name" value="Menaquinone_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030869">
        <property type="entry name" value="MqnD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37167">
        <property type="entry name" value="1,4-DIHYDROXY-6-NAPHTOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37167:SF1">
        <property type="entry name" value="1,4-DIHYDROXY-6-NAPHTOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02621">
        <property type="entry name" value="VitK2_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 3" id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="108"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="275" mass="30771" checksum="469B6804BF6AE3A1" modified="2018-07-18" version="1">MEISVGHTPDSDDAFMFYGMFTGKVPSPDFKVNHVIEDIEKLNRKATDPELDVTAVSVHACAYIPGYTILRSGGSFGIGYGPIVTAREQKSIDELKKCKIAIPGKMTSAFLLLQLMIGKFDFVEMNFSEIPEAVRTGKVDAGLVIHETQLSYAQEGNVKILDVGEWWDKTTDGLPVPLGINVMRTDLGMDTIVKFDKYLQSSIEFGLKNFDDALEYAMQYARGKKRDLIEKFVKMYVNQVTVNMGDPGEKSIRKLFEMAKEKKLVPDFQISIATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNY1</accession>
    <name>A0A2S2KNY1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_00750</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNY1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="107" mass="12822" checksum="4D854FDA51DF0D0D" modified="2018-07-18" version="1">MFEYDKHYKECRQLNKPFIKAKINPRHGNYYVQIDLITCNYELTDKGLVEIKKLFDKEIKFLNMSADFTNFSIDDELAWFDGVSIEHLDDFCNSLYDLTQKHRLKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNY4</accession>
    <name>A0A2S2KNY4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_00780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875955.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNY4"/>
    <dbReference type="OrthoDB" id="12178at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="461"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="461"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="483"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="496" mass="57612" checksum="66F9FC3723637AA8" modified="2018-07-18" version="1">MKQLTLTETIQKVESLIDTGNGDAGRLYHILEYLKSSRPLYHSDQVYLESKLDSSFSIYDDSVKKENDLLPKIQELIDSGKGDPGRLQHIYDTLESNKSLYHSDVSYLESKLNSDPQIKSVEEKKVLSPPPKEIVSSPPPKEIVSSPPPKEIVSSPPPKEIVSTIEPKTEIRGSMPKGWSPKNNSNELTEINADIKNEEAKIEQQEKISDEINHQRSKLTQLISHRKEYEQKITHEKSSLESQIKDERLRIEAQTKLSQEIISQKEELANVKKEREQIIKKIDVEKTKITRELNEQKKQLIQAQLDQEEIEKQVQKEQMLLAQMAEEQKSRLAEQAKIAHDIKLRQAEFEQTKKDYEDIVSQVNQEKAQFTESEKLEKILKKQEKELISAKEKRLNLISTISKEKELILKKTQEEKDKLKSQIKLNTQLKKEEKLFESLKKKRDKIEQQIKIKNQKLKSEQEKIKKQIDDKNKKLKSIEKKLKPSKKSSSKKTAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNZ5</accession>
    <name>A0A2S2KNZ5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01500</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876019.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNZ5"/>
    <dbReference type="GeneID" id="76209480"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="118" mass="13536" checksum="98C21A4B0E73817C" modified="2018-07-18" version="1">MFSPDEFLTFVKTIRRKNELQTEAGVRTALNRCYFSSLVKAKNHLESKGNNFSNNEEMHKEIIEKVKEANETMGDKLNTLLEMRNKADYDMEFNGDSGLISPIYGMSKSFNDKVSSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP34</accession>
    <name>A0A2S2KP34_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Proteasome endopeptidase complex,subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_00190</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875902.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP34"/>
    <dbReference type="GeneID" id="76209345"/>
    <dbReference type="OrthoDB" id="9421at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000004">
        <property type="entry name" value="Proteasome subunit alpha type-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019982">
        <property type="entry name" value="Proteasome_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03633">
        <property type="entry name" value="arc_protsome_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="5">
        <location>
            <begin position="8"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="245" mass="27111" checksum="D9FCA4EF1AC65277" modified="2018-07-18" version="1">MLPAQQGYDRAITVFSPDGRLYQVEYAIETVRRGTIAVGVKCKDGIIIAVEEKPRKLQISNTAQKIFQIDDHVGVAAAGYIPDARSQVDNARFFSQSNKMIYDEPVEVETIAKHLADQCQQYTQYAGVRPYGVALILGGVVNNTPQLYLTDPSGTYISYDAIAIGSGSDQVTDFLEKTYKDDLSLDEAATLAAAGIYLSSEDKEGTSHIRMAHIKTETELYELVSDEQIANYAKTAREKYPHDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP38</accession>
    <name>A0A2S2KP38_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_01900</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_146195961.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP38"/>
    <dbReference type="GeneID" id="76209521"/>
    <dbReference type="OrthoDB" id="3339at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="646" mass="73056" checksum="7B85BB3F9BFC54B5" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP79</accession>
    <name>A0A2S2KP79_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">2-alkenal reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_02840</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876139.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP79"/>
    <dbReference type="GeneID" id="76209621"/>
    <dbReference type="OrthoDB" id="350578at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06779">
        <property type="entry name" value="cpPDZ_Deg_HtrA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051201">
        <property type="entry name" value="Chloro_Bact_Ser_Proteases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43343">
        <property type="entry name" value="PEPTIDASE S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43343:SF3">
        <property type="entry name" value="PROTEASE DO-LIKE 8, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="266"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH33493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="380" mass="39767" checksum="FC306FD0697AC48D" modified="2018-07-18" version="1">MDKSGIIVGASVGAAIVLVLFAVLFISSPESIKPEIIVSNGHSSSTVGEVTSIYSKKLSLIEIFEKSEPGVVRVNVQRSETADIANGVGSGFVFDKIGHIITNAHVVKDANKVTITFLDGRSYNAEIIGSDEFTDIAVVKVNADLELLHPLSLGDSSNLKVGEQIAAIGNPFGLSGSMTSGIVSQLGRLLPSGNGYSIPDVIQTDAAINPGNSGGPLINMHGGIVGINTAIQSATGEFTGVGFAIPSQTVAKIVPILIEKGEYKHPWIGISGRDIDPDLAKVLELKDAVGFLVVTVVEDSPASKAGLIGSDKTIETDGISYAVGGDIILAVDGKEVRKIDDILIHLQRAKTIGDEMVLDILRDGRTTNVTVILEQRPNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP81</accession>
    <name>A0A2S2KP81_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Carbohydrate-binding module family 96 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_02710</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876129.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP81"/>
    <dbReference type="GeneID" id="76209608"/>
    <dbReference type="OrthoDB" id="4148at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055372">
        <property type="entry name" value="CBM96"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033679">
        <property type="entry name" value="DNRLRE_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24517">
        <property type="entry name" value="CBM96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate-binding module family 96" evidence="5">
        <location>
            <begin position="111"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="303" mass="32934" checksum="97B30BA44A24D169" modified="2018-07-18" version="1">MTTLTTTEHSGIKLLSVMVIAGVAFSGMTFFMLGDGFTSSLLSDKNYEMLCNTGLCFDPSSASFPTGLFIQQNQGFDSQMIGTNTDSFSAFRAVKTSVSDIQFTENSAILFSSKDSFVREGLQTSNEGSNKVLRIMGTGPTTNRALIGFDEIQLGTALGDKTLDSAKLKIFVVDNDGQWQEGQSVTVRSLTEPWQEGIGSNAPFGNFIGTQKGVTWNCSSEDNCENWNGGSFKATVADTVVISNDMSGKWIEFDVTEDILAYLDGTPNYGWIIMKSDEDSSGRINIAARETQDNIPQLELTFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP82</accession>
    <name>A0A2S2KP82_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Peptide ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_01220</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP82"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30465:SF45">
        <property type="entry name" value="BINDING-PROTEIN-DEPENDENT TRANSPORT SYSTEMS INNER MEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30465">
        <property type="entry name" value="INNER MEMBRANE ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="311"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="121"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="345" mass="38168" checksum="EC2AD75CBAF36994" modified="2018-07-18" version="1">MATRIATIFGVLIITLLITISLVGSNMDTILKQGIVFQVRSEITENPAIAKSFSSVDEFESFVQAQIEQRTKILGLDEPWYSPQRIGITMYKILLLDFGHATFLTSDEGSSNVKDILFEKLPRTVLLFTTATIIISIIGIFLGAFSSSKVGSVIDRITSSFAIISSSFPVWWIGMLMIFLFAFTYQIFPARATPDIPSSDPGYVGSLLYHMALPLITIVMIGFGSWAYLVRNFMVGIMQEDFIMAKKTIGISQKKIIYRHALKNAAPPIVTILALSLSGSLGGAIITEAVFDWPGMGRLYFEAITVMDLPVIIGATYLLTVFFLISIFIADLLYGYFDPRVRTGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPC4</accession>
    <name>A0A2S2KPC4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_02830</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876138.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPC4"/>
    <dbReference type="GeneID" id="76209620"/>
    <dbReference type="OrthoDB" id="46222at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00761">
        <property type="entry name" value="Glyco_tranf_GTA_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43646">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43646:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 2-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="291"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="325"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="349"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="7">
        <location>
            <begin position="54"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="402" mass="45191" checksum="87659DC72D047FA6" modified="2018-07-18" version="1">MELILDIFNYTLTAILIGICGAWVFLIKSMVDSFRFTPYLDKFENTSKTNPKVSVILPARNEEEFISKCLDSLIDQDYKNYEIIVIDDSSEDNTQKIIYKYAEKHSKVIPIIARPKPDGWMGKNWACMEGYKKASGELLLFTDADTKHSQNVISLAVGHLVSFNLDALSAIPKMMTFDFWTKVTLPMISTFLHSRFSALNVNNPAKKTGYFFGSFFILKKKTYENVGMHEGVKHEIIEDGALGKKVKEAGHKMKMVRGDHLIDAVWARDKSTLWNALKRLMIPLYLQNAKIAIGIFFAVAFLLFVPFPIFAVSLSIPIEEISSKILCISAAAASSLIYIGAIIETKMGLHLRLIHALFAPLGSLVVVLGFLSGLLQAKRNESVMWRGRSYSMKDHTQNSISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPG5</accession>
    <name>A0A2S2KPG5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_03100</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPG5"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00397">
        <property type="entry name" value="DNA_BRE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050090">
        <property type="entry name" value="Tyrosine_recombinase_XerCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30349:SF41">
        <property type="entry name" value="INTEGRASE_RECOMBINASE PROTEIN MJ0367-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30349">
        <property type="entry name" value="PHAGE INTEGRASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="5">
        <location>
            <begin position="129"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="333"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="380" mass="44382" checksum="8B509D75E5C98A3E" modified="2018-07-18" version="1">MIYCMIKSVLSKKPLVNSKFTMNNSLNQRSLLLYHSAIKSEKTQRQYDYQLKCFKEYSAIKSFDKLVEIEPKKLQTMIEDYVMYARDQGQGLSSIRAKLVALKLFFVMNDVLLNWDKLRKMIPEKTKSTGERPYTTQEIEILVKSSKTLRYRALMQFMASSGVRVGCFEELKMKHLKDMPNGCKSVLVYADTKDEYYTFIHQEAVEALDEYLEFRTRKGETITPDSWVFCSPCDTTKPSSTESTTTTLSRHVKRSLGREESKHGRYEIMTCHGFRKRFITILKSNPSVNISLAEKLAGHSVTIPLDNSYFKPLLEQLFDEYQKVIPKLFIDDKYRLQSQIQSQQDEIDLLQDKNTEIENLQKTILQIQNNMLELQKRHNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPJ1</accession>
    <name>A0A2S2KPJ1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Signal recognition particle 54 kDa protein</fullName>
            <shortName evidence="10">SRP54</shortName>
            <ecNumber evidence="10">3.6.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">srp54</name>
        <name evidence="15" type="ORF">NZNM25_03670</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.4"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9 10">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. Archaeal SRP consists of a 7S RNA molecule of 300 nucleotides and two protein subunits: SRP54 and SRP19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="10">The SRP-RNC complex is targeted to the cytoplasmic membrane.</text>
    </comment>
    <comment type="domain">
        <text evidence="10">Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.4" evidence="10"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876213.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPJ1"/>
    <dbReference type="GeneID" id="76209708"/>
    <dbReference type="OrthoDB" id="52849at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17875">
        <property type="entry name" value="SRP54_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000022">
        <property type="entry name" value="Signal recognition particle 54 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="entry name" value="Signal recognition particle, SRP54 subunit, M-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 54K PROTEIN SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF5">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="Signal peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 10" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="Signal recognition particle SRP54 helical bundle" evidence="14">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="12">
        <location>
            <begin position="98"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="13">
        <location>
            <begin position="99"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="106"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="188"/>
            <end position="192"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="246"/>
            <end position="249"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GBH33576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="441" mass="48757" checksum="58A241539334F351" modified="2018-07-18" version="1">MLDGLKNSLSDAIKKIVKSSGIDEELIKELSKDVQRALLQSDVNVRLVLEITKHLEERALNETPPPGLSRKDHIIKILYDELSKLLGKESEFDFKAGRQNKIILLGIQGSGKTTVASKLAKFLTRQGYKVGVIGADTYRPGALVQLRTMCEKSNVEVYGEENNKDSPSIVKNGLKHFEVQPLDVILIDTAGRHKEEQDLLEEMARINKAAEPDLALLVIDGTIGQQCFNQAEAFHKTVPVGGVIITKLDSSAKGGGALAASAATGAQIMYIGTGERIDDLEKFSPTRFVGRLLGMGDIQAVLDLAKRLENEGDDVRMKRISSGKMNMEDFFYQLEEVTKVGSLKGLLDSMPGFSGMVKNDQLDQMEDRVSKWRYIIQSMTKDEKADPDLINSSRIKRIARGSGWPEGEVKELLKNYKNSKSMMKASKGRQMQGTLRKMGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPL5</accession>
    <name>A0A2S2KPL5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Phosphoribosylformylglycinamidine cyclo-ligase</fullName>
            <ecNumber evidence="4 15">6.3.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 15">AIR synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13 15">AIRS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 15">Phosphoribosyl-aminoimidazole synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">purM</name>
        <name evidence="18" type="ORF">NZNM25_02980</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ATP = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the AIR synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.1" evidence="4 15"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876153.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPL5"/>
    <dbReference type="GeneID" id="76209635"/>
    <dbReference type="OrthoDB" id="6605at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00129"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046084">
        <property type="term" value="P:adenine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02196">
        <property type="entry name" value="PurM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000020">
        <property type="entry name" value="Phosphoribosylformylglycinamidine cyclo-ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.650.10:FF:000011">
        <property type="entry name" value="Phosphoribosylformylglycinamidine cyclo-ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00741">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004733">
        <property type="entry name" value="PurM_cligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00878">
        <property type="entry name" value="purM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520:SF12">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 15" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="16">
        <location>
            <begin position="44"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="17">
        <location>
            <begin position="176"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="GBH33507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="340" mass="36326" checksum="C28A22E59381E7A8" modified="2018-07-18" version="1">MVLTYKKAGVDISKIKQSQQAIGKLIASTHKLQKKAKIAHGFGHYAGIVEIPGGKLLATHTDGVGTKVIIANMMKKYNTIGIDCVAMNVNDVICIGATPISFVDYIAANKNDATIFKKIVEGLVTGAKKSSMPIVGGETAIMPDVIDGKGFAFDLAGMVVGLVDKKDLVLGNKINVGDVIVGANSTGIHSNGYSLARKAILGKYSIKDKVKGIGTIGNALLAPTEIYTNPVLEIIQKCKVNGLAHITGGAFTKLLRLKKIGYEIESLPKIPPIMGLVEEQGVKLEEMYKTFNMGVGFCVIAPKEQAVRIKSIFKKHKIQSQEIGKIIPKKGVFVNSIKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPP2</accession>
    <name>A0A2S2KPP2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">F420-0--gamma-glutamyl ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_02290</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876094.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPP2"/>
    <dbReference type="GeneID" id="76209563"/>
    <dbReference type="OrthoDB" id="11383at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052618">
        <property type="term" value="F:coenzyme F420-0:L-glutamate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.100">
        <property type="entry name" value="CofE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1660.10">
        <property type="entry name" value="CofE-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008225">
        <property type="entry name" value="F420-0_g-glutamyl_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002847">
        <property type="entry name" value="F420-0_gamma-glut_ligase-dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01916">
        <property type="entry name" value="F420_cofE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47917">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47917:SF1">
        <property type="entry name" value="COENZYME F420:L-GLUTAMATE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01996">
        <property type="entry name" value="F420_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144010">
        <property type="entry name" value="CofE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0630">Potassium</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Coenzyme F420:L-glutamate ligase-like" evidence="8">
        <location>
            <begin position="10"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="241" mass="26683" checksum="FF9658812CA2420B" modified="2018-07-18" version="1">MQIIPIFIEKEIEPSDNISELIIKSSELNNGDIIVIAQKIISKQEGRTVRLSDIVPSLLSQGIASQYQKDPRLIELILSETKRIVRMKNGIIIVETKNGFICANAGIDESNVKEGHATMLPINSDISAESIRYNILKQTNKNVAVIISDTFGRPFRMGQTNCAIGISGLNPIIDYAGTLDSFQKILRVTAIAVADELSSAAELVMGKSLKSPIAIIRGYPFKIEEHVIDELIRPEHEDLFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPP3</accession>
    <name>A0A2S2KPP3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphomethylpyrimidine synthase</fullName>
            <ecNumber evidence="9">4.1.99.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_03550</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.17" evidence="9"/>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPP3"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070284">
        <property type="term" value="F:phosphomethylpyrimidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.540">
        <property type="entry name" value="Radical SAM ThiC family, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038521">
        <property type="entry name" value="ThiC/Bza_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002817">
        <property type="entry name" value="ThiC/BzaA/B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009895">
        <property type="entry name" value="PRK13352.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00190">
        <property type="entry name" value="thiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30557:SF1">
        <property type="entry name" value="PHOSPHOMETHYLPYRIMIDINE SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30557">
        <property type="entry name" value="THIAMINE BIOSYNTHESIS PROTEIN THIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01964">
        <property type="entry name" value="ThiC_Rad_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00407">
        <property type="entry name" value="phosphomethylpyrimidine_syntha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01114">
        <property type="entry name" value="phosphomethylpyrimidine_syntha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00113">
        <property type="entry name" value="Radical_SAM_Phosphomethylpyrim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="NCBIfam" id="TIGR00190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH33564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="453" mass="49937" checksum="65147CD729618006" modified="2018-07-18" version="1">MFKSGKNIECVMATQMTSARRGVATDEMKQVAKDESVSLDWLIPKIASGSIIIPSNNARPQKIHNVGIGKGLKTKVNVNIGTSTLNVNLDEEIEKAKVAIKYHADTIMDLSDGGDVKKIRQTLMEHAPITFGTVPIYEAYNFGVEIHKNPLNLTEDDYLNAFENNAKDGVDYTTIHCGITKDIAKRILKVQRYGGVVSKGGTITAAWMLKHDKENPYLTHYDYLIEVAKKYDVTFSLGDALRPGSILDSHDELQVQEMINISQLTKRAHEQDIQVMVEGPGHVPLNEVAANVRLAKSLIGDVPYYVLGPLVTDIASGHDHIASAIGAAVSASEGVDLLCYLTPSEHLALPNAEEVKAGLIAYRIAAHAGDLVKIRDKVIKWDMEMTEARRTLDWEKQLALSIDPEEAAKIHSRTGQHPGNNVPCTMCGGACVYMMLPQQKKYEKENKNLQQIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPP9</accession>
    <name>A0A2S2KPP9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04330</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPP9"/>
    <dbReference type="OrthoDB" id="10119at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="290" mass="33339" checksum="C85C2CB8D9C38391" modified="2018-07-18" version="1">MDVKKFISEHELTSFPVGLGGCRTSEINDDSCDYDVFVFDGKSGQEVVPYENEFVMIHHASFSETQSKQLLQYDKLQIIQDDSWELRMLLSKINQKHSSLYTDSAKNSLIESLFCCQKTKESIQSSDVFGPCWQKCAAFNLADAITSLNNLRVSPSHMLDLLRRLEKNQINEHISIVSQTVGIERATPPLLERMLKSTIGFSDLVEKNNHSQTIKRKYEFFVKNSRLSDCYFYLGWINKENFLKIKNALNREQDHIHILKVAFDLEADMNLVEQQANLIQKSCNDILGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPR8</accession>
    <name>A0A2S2KPR8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Glycosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_04530</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876324.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPR8"/>
    <dbReference type="GeneID" id="76209805"/>
    <dbReference type="OrthoDB" id="46222at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06437">
        <property type="entry name" value="CESA_CaSu_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000057">
        <property type="entry name" value="Glycosyltransferase-like protein, family 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32044">
        <property type="entry name" value="GLUCOMANNAN 4-BETA-MANNOSYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32044:SF80">
        <property type="entry name" value="XYLOGLUCAN GLYCOSYLTRANSFERASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13641">
        <property type="entry name" value="Glyco_tranf_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="313"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="336"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="433"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="458"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="504"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="648"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH33662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="688" mass="77550" checksum="B36734C0F9EAA5B0" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPT9</accession>
    <name>A0A2S2KPT9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tetrapyrrole biosynthesis uroporphyrinogen III synthase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_04050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876285.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPT9"/>
    <dbReference type="GeneID" id="76209751"/>
    <dbReference type="OrthoDB" id="15395at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004852">
        <property type="term" value="F:uroporphyrinogen-III synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006780">
        <property type="term" value="P:uroporphyrinogen III biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06578">
        <property type="entry name" value="HemD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10090">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036108">
        <property type="entry name" value="4pyrrol_syn_uPrphyn_synt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003754">
        <property type="entry name" value="4pyrrol_synth_uPrphyn_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039793">
        <property type="entry name" value="UROS/Hem4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40082">
        <property type="entry name" value="BLR5956 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40082:SF1">
        <property type="entry name" value="BLR5956 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02602">
        <property type="entry name" value="HEM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69618">
        <property type="entry name" value="HemD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrapyrrole biosynthesis uroporphyrinogen III synthase" evidence="1">
        <location>
            <begin position="18"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="266" mass="29387" checksum="8E5F70339D84F631" modified="2018-07-18" version="1">MLDGKTIAITRSKDDASEFIALAEENHAKALPLPTIELVSKGEKIVDEFLESVEKYCPDYSVFMSSKAVKLLFDTAKDVGKLDKLQLAVANTIVMSVGPKTTAALESEGIKVNLQPTTFSSVGVGEEFTQINAVGKKVIVPRSGASTPFLKELLNKIGIDVLEIHLYDVCAFRDTTQWNEFRELFFQDKVDGIVFTSASSVRGFFEIMQKDFDESKLLENLQKLSVIAIGPFTADELKKFNVKNTVSQVHTVLGSFDTMKQILSDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPZ8</accession>
    <name>A0A2S2KPZ8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription initiation factor IIB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tfb_2</name>
        <name evidence="9" type="ORF">NZNM25_03820</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TFIIB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876227.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPZ8"/>
    <dbReference type="GeneID" id="76209724"/>
    <dbReference type="OrthoDB" id="7429at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000023">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="8">
        <location>
            <begin position="5"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH33591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="318" mass="35263" checksum="7D414AB316C2DDF6" modified="2018-07-18" version="1">MNILEKQNCPECKSTLVDDMQNGEIICSGCGVVVDDQISDYGPETISSNFEDKMKLARATGQTTYSQHDLGITTEISISAKDFSGKTINREVANQMHNLRKWQQRVRVSSPKERRLANVLTKMGETCDSLNLSKNVLETASMIYRNLDGHVDVKGKSVVSITAATIYMACKQCDVVRSLEEICRGVCPPKDVKSKTKLAARYYRTMVMEMGHLTAPVVTMDKYISKIANMTQTEVRVERLALEIAEKTKDSSIADGKAPNGIAAAYLYVASVLLGQNVLQRDVSSIAGVTEVTIRNRCKEILTCYKLKITLRPSLAKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ11</accession>
    <name>A0A2S2KQ11_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="16">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">NZNM25_05710</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The Rpo2 subunit (Rpo2N and Rpo2C in this organism) is implicated in DNA promoter recognition and in nucleotide binding.</text>
    </comment>
    <comment type="function">
        <text evidence="16">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 16">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="13">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="16"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876427.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ11"/>
    <dbReference type="GeneID" id="76209923"/>
    <dbReference type="OrthoDB" id="6009at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.270.10:FF:000011">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1800.10:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1100.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007646">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007647">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019969">
        <property type="entry name" value="RNAP_Rpo2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006335">
        <property type="entry name" value="PRK08565.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007175">
        <property type="entry name" value="PRK09606.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03670">
        <property type="entry name" value="rpoB_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04566">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04567">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 16 24" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 16" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 16" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="20">
        <location>
            <begin position="21"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="19">
        <location>
            <begin position="170"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="21">
        <location>
            <begin position="412"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="22">
        <location>
            <begin position="511"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="23">
        <location>
            <begin position="598"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="17">
        <location>
            <begin position="645"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="18">
        <location>
            <begin position="1020"/>
            <end position="1110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF04566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF04567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="GBH33780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="1115" mass="124645" checksum="30F9BAA5FE873B7C" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ12</accession>
    <name>A0A2S2KQ12_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ12"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="210" mass="24141" checksum="59285E1C23C87CAE" modified="2018-07-18" version="1">MDFDGMCSDDASERTDNVDEYRRTCIDFIEDISHDYEAWVDYYKLPEDEDKRRRAGIRATITRTNEWIAKVAQSIKAIDVVMNDGIQKMAESTAGKPLEIGVFVNHKSGYTETRPGIVDVNVKGHGRDGRKMKLGFHFKDDRFRVESTCDAYIDENDLPTQEFEMMDIHLKLHAENAKPRDVISFTVTISEVENDVEFDRRGVSTIVHLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ67</accession>
    <name>A0A2S2KQ67_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribosomal-protein-alanine N-acetyltransferase RimI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_06210</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876470.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ67"/>
    <dbReference type="GeneID" id="76209976"/>
    <dbReference type="OrthoDB" id="43754at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004596">
        <property type="term" value="F:protein-N-terminal amino-acid acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006464">
        <property type="entry name" value="AcTrfase_RimI/Ard1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045047">
        <property type="entry name" value="Ard1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01575">
        <property type="entry name" value="rimI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091:SF4">
        <property type="entry name" value="N-TERMINAL AMINO-ACID N(ALPHA)-ACETYLTRANSFERASE NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="11"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="165" mass="18974" checksum="FA805993764B0FDA" modified="2018-07-18" version="1">MQVILRQLGDCNIRRAEPSDLISVMEINLKTLPEHYSDYFYESLLAELPEAFIVAEIGGKHVGYIMCKTEYGFSNFKKLGFVKKGHVVSIAVLDEYRRKGIGQALVEESVNGVKLRKCDEFYLEVRCSNNEAVRLYEKLGFVIRQQLNAYYRDGEDAYLMAIELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ69</accession>
    <name>A0A2S2KQ69_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Haemin-degrading HemS/ChuX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_04880</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876356.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ69"/>
    <dbReference type="GeneID" id="76209840"/>
    <dbReference type="OrthoDB" id="4845at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1570.10">
        <property type="entry name" value="HemS/ChuS/ChuX like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053733">
        <property type="entry name" value="Heme_Transport_Util_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055136">
        <property type="entry name" value="LFE_1968-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22684">
        <property type="entry name" value="LFE_1968-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144064">
        <property type="entry name" value="Heme iron utilization protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="124" mass="14278" checksum="35945ECA7287E2EE" modified="2018-07-18" version="1">MLFELLSDIVATDDVLFIVKSNAATCEVRSDSLNIKQKEKWITIGDNDDPAHMHIDSELIKSAKFLQEEKPERISFSVQFFDGYGDRVLAAFFTKMYDGTKTIIPSRKKLYEDLNQKYSSIINF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ84</accession>
    <name>A0A2S2KQ84_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_06230</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876472.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ84"/>
    <dbReference type="GeneID" id="76209978"/>
    <dbReference type="OrthoDB" id="9050at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048068">
        <property type="entry name" value="LarA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043166">
        <property type="entry name" value="LarA-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33171">
        <property type="entry name" value="LAR_N DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33171:SF17">
        <property type="entry name" value="LARA-LIKE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="360" mass="39952" checksum="96AA1960D3653C3C" modified="2018-07-18" version="1">MPEIWLNYGVTDVVLDIRAENLEQKIDSDGKILEDSAINEKLNSLDLSKPMELVVLHNSKSIQKIISSLFTLCEQKSKPFPKIFAEKKILNLVKAGLPEGSSINEFDSADISNSNLVFMGEMEFDGLFGYETISTRLIKKFGLDSMLSAYAKRQSNLPVPGQHTESLVEAKKFTDNFEIQGIEIVANSKGILDFSIGHPSETLSTSKILESNSIKDVGEHKTMIISTGKDASNFHLGKSLSSLWNCYGAIKKDGLAILVAECKGGLGSDAIQQYIEDRLTLEQLRNPTKYLSGMEDLLFLTEVQKNFQIGLVSILPEFYAKKLNMISLPGIKYSMDYILKTQGVRQKVAVVTDGARLLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQC5</accession>
    <name>A0A2S2KQC5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">NZNM25_06810</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the MCM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876519.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQC5"/>
    <dbReference type="GeneID" id="76210041"/>
    <dbReference type="OrthoDB" id="6747at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17706">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000826">
        <property type="entry name" value="Replicative DNA helicase Mcm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF66">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="10">
        <location>
            <begin position="300"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH33890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="695" mass="78361" checksum="32A2CBDC4D173FC9" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQD3</accession>
    <name>A0A2S2KQD3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">succinate dehydrogenase</fullName>
            <ecNumber evidence="5">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">NZNM25_06640</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate metabolism; tricarboxylic acid cycle.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="5"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876503.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQD3"/>
    <dbReference type="GeneID" id="76210023"/>
    <dbReference type="OrthoDB" id="144910at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1060.10:FF:000003">
        <property type="entry name" value="Succinate dehydrogenase iron-sulfur subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050573">
        <property type="entry name" value="SDH/FRD_Iron-Sulfur"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004616">
        <property type="entry name" value="PRK05950.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921:SF29">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] IRON-SULFUR SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE IRON-SULFUR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="13" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="15">
        <location>
            <begin position="20"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="16">
        <location>
            <begin position="158"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GBH33873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="255" mass="28694" checksum="F455EB1D6C2CA9C9" modified="2018-07-18" version="1">MAQVSHIANEQTSTPISSSKSVTLRIARYNPEHDDSSKFMEFTIPYERWTTVLEAILEVKKHLDHSVAVRYSCRQATCGSCGMMINGKPRLACFTKISELNSDVITVEPMNNFPIIRDLAVKFERLFDTHHKIKPYLIRDDADLVSDEREFLQSPEEVEQYIQFANCIKCGLCNSACPTMATDSSFVGPQALAQAYRYVADSRDKGKDSRLKIIDESHGIWRCHFAGSCSQVCPKGVDPAMGIQLLRGYLLGFRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQD5</accession>
    <name>A0A2S2KQD5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sulfurtransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_04560</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876550.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQD5"/>
    <dbReference type="GeneID" id="76209808"/>
    <dbReference type="OrthoDB" id="10492at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01448">
        <property type="entry name" value="TST_Repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01449">
        <property type="entry name" value="TST_Repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051126">
        <property type="entry name" value="Thiosulfate_sulfurtransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001307">
        <property type="entry name" value="Thiosulphate_STrfase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43855">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43855:SF1">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00683">
        <property type="entry name" value="RHODANESE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="20"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="159"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="281" mass="32126" checksum="954BB16E985F4179" modified="2018-07-18" version="1">MSYAHPEVLVDTEWVSQNPPNENRKLVEVDYDPVNGYQKGHINGATLIWWKRDINDPVTRDIIGKKQFEALMAKNGITADTEVILYGDFNNWFAAFVFWVFKIYGHENLKIMNGGRKKWELENKSYTTEEPQLSPSTYIAQPPDEGLRAYLFDVSRALDKEDTVMVDVRSPAEFTGQITAPPEYPMEHAQRGGHIPGANNIPWATAVNDADGTFKAVEELRQNYEPKGVTPDKDVICYCRIGERSSHSWFVLKYLLGYPKVRNYDGSWTEWGNMIGNPVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQE6</accession>
    <name>A0A2S2KQE6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_06770</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876515.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQE6"/>
    <dbReference type="GeneID" id="76210037"/>
    <dbReference type="OrthoDB" id="30669at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03358">
        <property type="entry name" value="LbH_WxcM_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050179">
        <property type="entry name" value="Trans_hexapeptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43300:SF10">
        <property type="entry name" value="2,3,4,5-TETRAHYDROPYRIDINE-2,6-DICARBOXYLATE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43300">
        <property type="entry name" value="ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14602">
        <property type="entry name" value="Hexapep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="158" mass="17234" checksum="71FAC6440C3AA5E0" modified="2018-07-18" version="1">MVTNFISDKAKIGQNVQIWHFSYVGDNVEIGDNVKIGSLAHIDYDVKIGENTKIEGQAYIPPLSRIGKNVFIGPAAALTNDPYPMCDKMIGVTIEDNVIIGARAVIKAGVTVRKNSVVAMGAVVTRDVPENSVVIGSPATIRYSRGEYDKKQRKWKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQG3</accession>
    <name>A0A2S2KQG3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Fructose 1,6-bisphosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_06450</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876489.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQG3"/>
    <dbReference type="GeneID" id="76210002"/>
    <dbReference type="OrthoDB" id="58111at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008934">
        <property type="term" value="F:inositol monophosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006020">
        <property type="term" value="P:inositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000027">
        <property type="entry name" value="Fructose-1,6-bisphosphatase/inositol-1-monophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000020">
        <property type="entry name" value="Fructose-1,6-bisphosphatase/inositol-1-monophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854">
        <property type="entry name" value="INOSITOL MONOPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854:SF4">
        <property type="entry name" value="INOSITOL-1-MONOPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH33854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="266" mass="29292" checksum="25BB7B59A923E625" modified="2018-07-18" version="1">MQTVDILREASNRIFENVKNLAGTEHAAGDFGIGAGGDISRNIDIVAEKTVLDYLKEINFECIVLGEECGRVELSDNPKGFIIMDAIDGSANAVRGIPFFCSSLAFATENKLSSITDGVITNLANGEMYWASKNKGSFFNDQQIRVRSNDPIYKIVGVNISGASDNLVKRVLPIFEKHEHLRHFGANALEMAFFARGLLDIFIDLREKIRIQDIAAGYLIVREAGGLLLDSDLNPLDADLSYDTRISFIAASNQKILDEIMSLIDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQI0</accession>
    <name>A0A2S2KQI0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF72 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07000</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876534.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQI0"/>
    <dbReference type="GeneID" id="76210061"/>
    <dbReference type="OrthoDB" id="35747at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.410">
        <property type="entry name" value="Protein of unknown function UPF0759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002763">
        <property type="entry name" value="DUF72"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036520">
        <property type="entry name" value="UPF0759_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30348:SF4">
        <property type="entry name" value="DUF72 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30348">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YECE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01904">
        <property type="entry name" value="DUF72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117396">
        <property type="entry name" value="TM1631-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="263" mass="30657" checksum="539DFB652FE5EF50" modified="2018-07-18" version="1">MNIKIGCTGWSYKGWSDTFYPRNLKSSEWLKYYSQIFEITEINSTFYKIPSQEIVRKWNADTPRHFRFTAKFPALITHEKRLERVNSEIFSFMSSLIPIHEKISALVLQLPPSLSFDEAKPRLEELFDILPNDFLYPIEGRHESWFTDEATSYLKQNKHCLVWNDVEGVSNPMPVTASYLYVRLIGDHSIPDSEFGKVSKSKKEAIRNWAERLGNIQDVPLAMVMTNNHYEGFGPATANSLRMELGMRELMWDEKKQKTLGNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQI1</accession>
    <name>A0A2S2KQI1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sodium:calcium antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">yrbG_2</name>
        <name evidence="7" type="ORF">NZNM25_04960</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876361.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQI1"/>
    <dbReference type="GeneID" id="76209848"/>
    <dbReference type="OrthoDB" id="142185at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008273">
        <property type="term" value="F:calcium, potassium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004481">
        <property type="entry name" value="K/Na/Ca-exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00367">
        <property type="entry name" value="calcium/sodium antiporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846:SF8">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YRBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846">
        <property type="entry name" value="SODIUM/POTASSIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="238"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="268"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="297"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="6">
        <location>
            <begin position="7"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="6">
        <location>
            <begin position="172"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="315" mass="33047" checksum="5E47D171C91E9607" modified="2018-07-18" version="1">MELIFNFLLVGAGLTMLYFGGDWLVKGSIALSNKFGISQLVIGLTVVAFGTSAPELAVSISSAMQGLSDIALGNVVGSNIVNIGAILGMSAIISPIIVSKSAIRKEVPIMIGISFLLLGIIIDGKIDFVDGALFVIGIIVFTGYSYRSSKKDTDTQVIPASQILQKNVFSKSIIFIIVGLLLLTGGSFLTVDNVVIIGASFGISELFMGLTVVAIGTSLPELITSVVAARKGHADLSVGNIIGSNIFNILAILGISSLISGITVTEQVLVDVGIMLAFSLVLIPIMRSGFVISRREGVILVAGYVVYVIFLFSRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQI3</accession>
    <name>A0A2S2KQI3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_07140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQI3"/>
    <dbReference type="OrthoDB" id="3300at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="77" mass="9137" checksum="84B242FBDFA767F6" modified="2018-07-18" version="1">MCSNYINLNTMWDKIDLESSNKENDIIGWKQEYMKNIVENLIELKIRAKKENNPSLYDQLRISLIRAHEVQNGLNQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQK3</accession>
    <name>A0A2S2KQK3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NADH-quinone oxidoreductase subunit L</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">NZNM25_06280</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876476.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQK3"/>
    <dbReference type="GeneID" id="76209985"/>
    <dbReference type="OrthoDB" id="371891at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018393">
        <property type="entry name" value="NADHpl_OxRdtase_5_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003945">
        <property type="entry name" value="NU5C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001516">
        <property type="entry name" value="Proton_antipo_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01974">
        <property type="entry name" value="NDH_I_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829:SF2">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00662">
        <property type="entry name" value="Proton_antipo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01434">
        <property type="entry name" value="NADHDHGNASE5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="205"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="316"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="341"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="374"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="418"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="458"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="504"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="567"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="615"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="674"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="domain" description="NADH-Ubiquinone oxidoreductase (complex I) chain 5 N-terminal" evidence="7">
        <location>
            <begin position="79"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="6">
        <location>
            <begin position="190"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH33837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="692" mass="75604" checksum="8E37FD9113563002" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQR2</accession>
    <name>A0A2S2KQR2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_07870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876642.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQR2"/>
    <dbReference type="GeneID" id="76208889"/>
    <dbReference type="OrthoDB" id="10393at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="318" mass="35322" checksum="2654333326FAF2DE" modified="2018-07-18" version="1">MKTKFVLIIIIVVLVAMYFLMHARIWPEPEPCDVGFEHYHFGFCSTNLFSVSGAFAQTDDNSVVSFAWEDGIDHKNTLGNIGEMYWLQASYSGKENSVATLRVIDPDLVQFPDSVDFIRIHVHSDTDPLGTDIVLTNTPENPGFFEGDVIFTHDFSSSRGVLLVSDGDTISAKYVDTTLPDDADSETYEVVATAIISGPRGPIEMVLATSLRIEDLKQNRFSDNVIPLGHQVLFSADLNAQTQDIHNFAYLVQVQDEKGRTMSLSWLSGTILPKQTITTSQSWIPDLAGKYFVTVFVWESVDNPTALSPPLSVEFVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQX1</accession>
    <name>A0A2S2KQX1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876659.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQX1"/>
    <dbReference type="GeneID" id="76208869"/>
    <dbReference type="OrthoDB" id="3229at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="62" mass="7205" checksum="098CCBDE3032FAB8" modified="2018-07-18" version="1">MKPVKKFPKGRFSSSLWNHIEQIQKSDGIDTHYENEIMLNDLDLFVNQKSRRGQQNRCGAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQX3</accession>
    <name>A0A2S2KQX3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">K(+)-insensitive pyrophosphate-energized proton pump</fullName>
            <ecNumber evidence="9">7.1.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Membrane-bound proton-translocating pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Pyrophosphate-energized inorganic pyrophosphatase</fullName>
            <shortName evidence="9">H(+)-PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">hppA</name>
        <name evidence="10" type="ORF">NZNM25_08710</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Proton pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for proton movement across the membrane. Generates a proton motive force.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>diphosphate + H2O + H(+)(in) = 2 phosphate + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:13973"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="7.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-insensitive subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.3.1" evidence="9"/>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876898.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQX3"/>
    <dbReference type="GeneID" id="76208799"/>
    <dbReference type="OrthoDB" id="53167at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009678">
        <property type="term" value="F:diphosphate hydrolysis-driven proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01129">
        <property type="entry name" value="PPase_energized_pump"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004131">
        <property type="entry name" value="PPase-energised_H-pump"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001960">
        <property type="entry name" value="PRK00733.3-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01104">
        <property type="entry name" value="V_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31998">
        <property type="entry name" value="K(+)-INSENSITIVE PYROPHOSPHATE-ENERGIZED PROTON PUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03030">
        <property type="entry name" value="H_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001265">
        <property type="entry name" value="H+-PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="57"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="121"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="245"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="307"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="394"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="462"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="500"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="574"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="658"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="site" description="Determinant of potassium independence" evidence="9">
        <location>
            <position position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="679" mass="69655" checksum="4F14A7CA0E32D98A" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQY7</accession>
    <name>A0A2S2KQY7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Flavodoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">isiB</name>
        <name evidence="8" type="ORF">NZNM25_08600</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the flavodoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876895.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQY7"/>
    <dbReference type="GeneID" id="76208811"/>
    <dbReference type="OrthoDB" id="431at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050619">
        <property type="entry name" value="Flavodoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010086">
        <property type="entry name" value="Flavodoxin_lc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01752">
        <property type="entry name" value="flav_long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006738">
        <property type="entry name" value="PRK09267.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42809:SF1">
        <property type="entry name" value="FLAVODOXIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42809">
        <property type="entry name" value="FLAVODOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038996">
        <property type="entry name" value="FldA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0288">FMN</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="7">
        <location>
            <begin position="3"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="174" mass="18986" checksum="B80164F3E92223E0" modified="2018-07-18" version="1">MTIGIYFGSQTGKTEMVCAKIKNELGDLSADPKDVSEVRGDISQFLEMDGLICAIPTWNTGEEENRSGTAWDELLEKIGNLDLRGKPVAICGTGDAAGYADNFVDAMEELHRYFEKAGAKMVGYVSTDGYTFTGSKSIIDGKFCGLPVDEDGESDQTEERVKLWCTQLKSEMGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR20</accession>
    <name>A0A2S2KR20_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_08970</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876735.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR20"/>
    <dbReference type="GeneID" id="76208772"/>
    <dbReference type="OrthoDB" id="3301at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="292" mass="32740" checksum="6F3B486F9F72CEBD" modified="2018-07-18" version="1">MKYAFIFLVLTGLVTSPYLVSDVSALCVINEDWPDQPCFSGRRGVDPSLNQMKQGWAPYYEFKGAELMESQKQELLQTIQNGNLLEWKKGDPSGAHHNVYMYYFLMGDIPNEDGLFAEEYYGVRLLSPLRQSDSGIAFDEIHCGIDLILVQKYDDSPACVTESTKQKLIERGWITTKDDSDVFDSIPAFDYEIKTSTGVTHGSQYFISGALVDEISYDEDRNSLTVLMTSSQSGILQIVMPIGLLHLPTQIPSYVVIADGEEIEYEKLTPILLKISFDSGTEELEIIGTLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR24</accession>
    <name>A0A2S2KR24_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4443 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_07980</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR24"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="entry name" value="GAD-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029349">
        <property type="entry name" value="DUF4443"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14544">
        <property type="entry name" value="DUF4443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4443" evidence="5">
        <location>
            <begin position="57"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="158" mass="17719" checksum="EAF9053FB4C277D9" modified="2018-07-18" version="1">MGEGAVRTLISYLREYEFVDSIKAGTFLTIKGKNFAKKFQEAIPYQSFLKKCCLTNGDHNCAIILRKEHVSNIGNGMKQRDYAILYGADDVLTLRFKNGEFVFVGDEVVCFADESEIKENLIEALHPRNNDIIIITSSNDKFVAEISAINTALWTLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR30</accession>
    <name>A0A2S2KR30_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">4Fe-4S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_08650</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876897.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR30"/>
    <dbReference type="GeneID" id="76208806"/>
    <dbReference type="OrthoDB" id="23833at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050572">
        <property type="entry name" value="Fe-S_Ferredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687">
        <property type="entry name" value="ADENYLYLSULFATE REDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687:SF6">
        <property type="entry name" value="L-ASPARTATE SEMIALDEHYDE SULFURTRANSFERASE IRON-SULFUR SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="9">
        <location>
            <begin position="62"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="9">
        <location>
            <begin position="123"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="177" mass="19825" checksum="0631C10F725AC577" modified="2018-07-18" version="1">MPIDESFPEGMEPIGKIMHSDGENFHWFWGPGKLKEASTHSKTVKDFAKRGEKIEPLGVSCTMVAVYWDSCYADGACIEACPVQVFQWYRTEKDIPAKSVIGETFDGTGENSDKEGRKDYTDKSDPIREHDCIWCMICVSVCPPQAIKVDQSNHEFHEKAAGSYQKLGSGQPNPHVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR58</accession>
    <name>A0A2S2KR58_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR58"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="62" mass="6797" checksum="7F2D3FBF9657CE09" modified="2018-07-18" version="1">MMFYATGIVGIVVGLAVAPPSMTVMITFMALINVGLGAFFTFIFLTQVQKAPDKRKKKRKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KR66</accession>
    <name>A0A2S2KR66_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876695.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KR66"/>
    <dbReference type="GeneID" id="76208823"/>
    <dbReference type="OrthoDB" id="8698at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="162" mass="17772" checksum="4A3FE3B79958AB85" modified="2018-07-18" version="1">MNKTKKKFSKLSKTNIISVSVVAGVVILIGGIALNSLSPVNYDGFVFAPSTNVFLKAVKSPQGIYHYQLTKGGKALQSSSETSPSIIMSKGNLVQIHLINEEKNQLDNPSKHNLNIDEFNVHIKDLSYFQSESVTFLADKTGTFDYYCSIHPGMKGTITITE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRB6</accession>
    <name>A0A2S2KRB6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_09800</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876809.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRB6"/>
    <dbReference type="GeneID" id="76208689"/>
    <dbReference type="OrthoDB" id="36796at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018973">
        <property type="entry name" value="MZB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47957">
        <property type="entry name" value="ATP-DEPENDENT HELICASE HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47957:SF3">
        <property type="entry name" value="ATP-DEPENDENT HELICASE HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09369">
        <property type="entry name" value="MZB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="3">
        <location>
            <begin position="148"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="4">
        <location>
            <begin position="346"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="836" mass="95681" checksum="6A5AD6C1E6FB405F" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRC2</accession>
    <name>A0A2S2KRC2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_07890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH33998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876644.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRC2"/>
    <dbReference type="GeneID" id="76208887"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="77" mass="8612" checksum="7AF624BA518B1525" modified="2018-07-18" version="1">MKIIFLILIVFSFIPFIIPNVFAICAAETLEWWEVCNDTGPENALPLHPVLLFMVILVITIGSIIGGLVFVIRGNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRG2</accession>
    <name>A0A2S2KRG2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">30S ribosomal protein S30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_10610</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048115322.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRG2"/>
    <dbReference type="GeneID" id="24819740"/>
    <dbReference type="OrthoDB" id="28200at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006846">
        <property type="entry name" value="Ribosomal_eS30"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04758">
        <property type="entry name" value="Ribosomal_S30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="60" mass="6722" checksum="DDBABB10EC257DDD" modified="2018-07-18" version="1">MATHGSLTKAGKVRGQTPKVEGRKIVGTSSSLRNKSNFKKRFVLGRFPGQNKPGQRRKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRG9</accession>
    <name>A0A2S2KRG9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_10340</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_225866859.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRG9"/>
    <dbReference type="GeneID" id="76208631"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="59" mass="6342" checksum="ECC040FA6F56BEB9" modified="2018-07-18" version="1">MDLKDDAVLATIGPSETNADYVPSVLCLSCETLMIITTHSELVVGIHHTVGNQVTMRNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRH8</accession>
    <name>A0A2S2KRH8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_10050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_160049212.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRH8"/>
    <dbReference type="GeneID" id="76208662"/>
    <dbReference type="OrthoDB" id="2985at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="51" mass="5892" checksum="25C64B1FA7329318" modified="2018-07-18" version="1">MLVETIQTEEISQLDKLINEVVEQRKVIGIKLTVTAFQEKLVYTVLVMLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRI6</accession>
    <name>A0A2S2KRI6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">AsnC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">asnC</name>
        <name evidence="5" type="ORF">NZNM25_09420</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876776.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRI6"/>
    <dbReference type="GeneID" id="76208728"/>
    <dbReference type="OrthoDB" id="14434at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053483">
        <property type="entry name" value="HTH-Lysine_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040947">
        <property type="entry name" value="trans_reg_LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154">
        <property type="entry name" value="LEUCINE-RESPONSIVE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154:SF50">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13404">
        <property type="entry name" value="HTH_AsnC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="139" mass="15289" checksum="B65631F01275F1FA" modified="2018-07-18" version="1">MYKDKVDEKIIGYLKEDARESFVDIGKKLKLSESAVRRRVKNLVDSGTIKKFTLELGEENATSAIVLVSVDSATDTSKVSLKLAKLEGVKTVYEITGQYDITTIISASSIAEINNTIDALRKIPGVVDTNTVIILRKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRJ9</accession>
    <name>A0A2S2KRJ9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription elongation factor Spt4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">spt4</name>
        <name evidence="4" type="ORF">NZNM25_08760</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Stimulates transcription elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer composed of Spt4 and Spt5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal Spt4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876714.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRJ9"/>
    <dbReference type="GeneID" id="56068308"/>
    <dbReference type="OrthoDB" id="275101at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00949">
        <property type="entry name" value="Spt4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022800">
        <property type="entry name" value="Spt4/RpoE2_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007178">
        <property type="entry name" value="Spt4_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038589">
        <property type="entry name" value="Spt4_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041664">
        <property type="entry name" value="RNAP_arch_Epp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40704">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40704:SF1">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06093">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01389">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="RNA polymerase subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Spt4/RpoE2 zinc finger" evidence="3">
        <location>
            <begin position="4"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="62" mass="6693" checksum="F46A36E674DD0A65" modified="2018-07-18" version="1">MAREMACRKCKFVTTGKVCPGCKSSDLTPDWSGIVLVVDPTNSEISKTLGITQKGKYAIKVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRM9</accession>
    <name>A0A2S2KRM9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876968.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRM9"/>
    <dbReference type="GeneID" id="76208534"/>
    <dbReference type="OrthoDB" id="383935at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="66" mass="7511" checksum="6769765C38CCF18F" modified="2018-07-18" version="1">MARKKLTEKEIEQQKEEQKIRAEASRAARRSGKSIKPEGKVTKSKAKTQEARSSKKNAAVRKRSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRN0</accession>
    <name>A0A2S2KRN0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">flpA</name>
        <name evidence="8" type="ORF">NZNM25_10650</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with nop5. Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the methyltransferase superfamily. Fibrillarin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="BGKI01000005">
        <property type="protein sequence ID" value="GBH34274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876921.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRN0"/>
    <dbReference type="GeneID" id="76208595"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990259">
        <property type="term" value="F:histone H2AQ104 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008649">
        <property type="term" value="F:rRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00351">
        <property type="entry name" value="RNA_methyltransf_FlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000692">
        <property type="entry name" value="Fibrillarin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003276">
        <property type="entry name" value="PRK04266.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335:SF17">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335">
        <property type="entry name" value="RRNA 2-O-METHYLTRANSFERASE FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01269">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006540">
        <property type="entry name" value="Nop17p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00052">
        <property type="entry name" value="FIBRILLARIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01206">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7" id="KW-0819">tRNA processing</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="93"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="112"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="138"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="158"/>
            <end position="161"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GBH34274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="236" mass="27245" checksum="4F0ACF36FB633C29" modified="2018-07-18" version="1">MKEQIQIKREKSLEEDNQSFFWIKSEGERKLATENLVPGNQVYKEKLIIKKGIEYRLWDAFRSKLAAAIMNELEYFPFENKSKVLYLGASTGTTVSHISDIVGPSGMVFAVEHASRVARDFLDRVASYRSNIMPILQDARKPKEYFSVFGKVDIVYVDIAQPDQTQIALDNCEMYLKNGGYFFLVIKTRSIDVTKSPRRIVEEETEKLKANFDILQSIDLHPYDKDHAMVIAKYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRQ1</accession>
    <name>A0A2S2KRQ1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_11270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876970.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRQ1"/>
    <dbReference type="GeneID" id="76208530"/>
    <dbReference type="OrthoDB" id="3193at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="73" mass="8478" checksum="1859E0E4767A5BE8" modified="2018-07-18" version="1">MAIPLIVLVGIPIFLGIVYVTPFDQPEEIEKDVISEEPDMSILYFILMGIWTLYLIKILFQIKKGNFKATQRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRS4</accession>
    <name>A0A2S2KRS4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876983.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRS4"/>
    <dbReference type="GeneID" id="76208516"/>
    <dbReference type="OrthoDB" id="8613at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="187" mass="22799" checksum="F18FE1BAD9B9F56C" modified="2018-07-18" version="1">MILHFIFVIKEEDREKRKFEFEYIKKMGQFYKVWIKEKFGRDYEIQCDELITTPRSILQRLDIPTFIRDHEQRGKDIYHFYLTHFKPLWTDCTCDGYNAENFGLVYWQPLQGNDPLFLAEKNCTSVSHVLLHEFLRILGHKKFIQEVHDIWTKHLFEQLEFEQYGEDFQKTDDKPMFLTMDTSQLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRZ3</accession>
    <name>A0A2S2KRZ3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Magnesium transport protein CorA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">corA</name>
        <name evidence="9" type="ORF">NZNM25_12140</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Mediates influx of magnesium ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877062.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRZ3"/>
    <dbReference type="GeneID" id="76209290"/>
    <dbReference type="OrthoDB" id="28779at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015087">
        <property type="term" value="F:cobalt ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12828">
        <property type="entry name" value="TmCorA-like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.340:FF:000012">
        <property type="entry name" value="Magnesium transport protein CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.20">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045861">
        <property type="entry name" value="CorA_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004488">
        <property type="entry name" value="Mg/Co-transport_prot_CorA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00383">
        <property type="entry name" value="corA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46494">
        <property type="entry name" value="CORA FAMILY METAL ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46494:SF1">
        <property type="entry name" value="CORA FAMILY METAL ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143865">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="491"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="522"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="548" mass="62027" checksum="C97A559C9091971F" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS03</accession>
    <name>A0A2S2KS03_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">acetate--CoA ligase</fullName>
            <ecNumber evidence="2">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_12270</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS03"/>
    <dbReference type="OrthoDB" id="371752at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF14">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="8">
        <location>
            <begin position="32"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="6">
        <location>
            <begin position="89"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="7">
        <location>
            <begin position="531"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="641" mass="72213" checksum="5727A0596FD6A1BE" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS08</accession>
    <name>A0A2S2KS08_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">MarR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">NZNM25_10290</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876849.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS08"/>
    <dbReference type="GeneID" id="76208636"/>
    <dbReference type="OrthoDB" id="10712at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42756">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42756:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR OF EMRAB OPERON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH marR-type" evidence="5">
        <location>
            <begin position="7"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="159"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GBH34238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="172" mass="19564" checksum="1C45062C861C17F5" modified="2018-07-18" version="1">MKTLDLKDSIGLMMKRSAKAWERATDIELTERFGLTGGKWKVITGLSIKEGITQKQLADMMFVEAPTLVPIIDRLEKEGYLTRQSDPKDRRNNLIFLTKKAKKTVDPIIDCIAKMRDIGLDKISKKDLEITKKTLTQINANAEAFIMEKGKKTEPDVWTNPQNKSQKTLVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS16</accession>
    <name>A0A2S2KS16_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11480</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000006">
        <property type="protein sequence ID" value="GBH34357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876989.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS16"/>
    <dbReference type="GeneID" id="76208508"/>
    <dbReference type="OrthoDB" id="6558at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="109" mass="12861" checksum="A1ABA79C9D8F1B7C" modified="2018-07-18" version="1">MAIINHMMKKIDTDVSNLKQGLHPQNLSYWYGKIIKETIEMAPPWLQDKIKVHQDPILSMKFNLDISKRAVRYFMIVVDNNLDEMPYSTKLYFLKVQEILSTEMDKSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS67</accession>
    <name>A0A2S2KS67_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein-disulfide isomerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_12450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877088.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS67"/>
    <dbReference type="GeneID" id="76209259"/>
    <dbReference type="OrthoDB" id="10863at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="198" mass="22246" checksum="E6676CA6780963B3" modified="2018-07-18" version="1">MGRKERRERELKRDNYATRHSAEKRKQTLIAIGVLAVIAVIVGYAVWIFINMSDNVPGGPENAGALGSEHSHAGLLVSIFGDEFDFSAPAYQIKSSWIHFEGRDGSTVHKHATGVTLGYLFDTLSLGLDDQCFVFQDGRSFCTNEDYKLVFYINGEQVPDIRDYEIQEDDKILIAYGAETPEEIDALLLKLESRELIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS81</accession>
    <name>A0A2S2KS81_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Anthranilate phosphoribosyltransferase</fullName>
            <ecNumber evidence="2 8">2.4.2.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 11" type="primary">trpD</name>
        <name evidence="11" type="ORF">NZNM25_12900</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'-phosphoribosyl)-anthranilate (PRA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N-(5-phospho-beta-D-ribosyl)anthranilate + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + anthranilate</text>
            <dbReference type="Rhea" id="RHEA:11768"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 2 magnesium ions per monomer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the anthranilate phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.18" evidence="2 8"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877118.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS81"/>
    <dbReference type="GeneID" id="76209210"/>
    <dbReference type="OrthoDB" id="8214at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00041"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004048">
        <property type="term" value="F:anthranilate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1030.10:FF:000002">
        <property type="entry name" value="Anthranilate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.970.10">
        <property type="entry name" value="Transferase, Pyrimidine Nucleoside Phosphorylase, Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00211">
        <property type="entry name" value="TrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005940">
        <property type="entry name" value="Anthranilate_Pribosyl_Tfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01245">
        <property type="entry name" value="trpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285">
        <property type="entry name" value="ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285:SF2">
        <property type="entry name" value="ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="4 8 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 8" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Glycosyl transferase family 3 N-terminal" evidence="10">
        <location>
            <begin position="4"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 3" evidence="9">
        <location>
            <begin position="74"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="84"/>
            <end position="85"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="91"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="109"/>
            <end position="117"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="346" mass="37746" checksum="29C79C85375DFA49" modified="2018-07-18" version="1">MISEIIAKLQQKTDLNYDEMNSVMTDILSGKTDDEQNTEFLSCLAEKGETDDELLGMLDKMQEFSLKVQTKNRGTIIDMCGTGGDKIRTFNVSTTASFVVAAAGGIVAKHGNRSSSGISGSADIFEYFGYDLNLEPAQISEILLKHNICFMFAQKFHPAMKHVSKVRKQLGRRTAFNLLGPLSNPAGVKNQLIGVSSIEYLDRLPAILKRKGSQNIMTVRSDDGMDEFSTSAANRVCILRNNKVLMNVIDPVVVGLHKSRLEDIQIKTKEEAIKSFVGVLNNTATQAMIETTALNAAGGLIVADIANNFEEGVDIALNTIKDGKAFSLLEKFVADTGDISKLNELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS96</accession>
    <name>A0A2S2KS96_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MarR family transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_13140</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877138.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS96"/>
    <dbReference type="GeneID" id="76209185"/>
    <dbReference type="OrthoDB" id="12000at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004977">
        <property type="entry name" value="Ribosomal_eS25"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03297">
        <property type="entry name" value="Ribosomal_S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="11"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="103" mass="10875" checksum="27B152E51EB52567" modified="2018-07-18" version="1">MGGAKKPTAASKDKSTATKDTKKSKKDKGESGPKKAEIIVMVNEQQAMKIIQSSKVVTVQDLARQTGVKISAANAFLKNSAIKGTVKRVGGYSGHHLYQAVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KS98</accession>
    <name>A0A2S2KS98_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_13100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877135.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KS98"/>
    <dbReference type="GeneID" id="76209189"/>
    <dbReference type="OrthoDB" id="1076at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="62" mass="7190" checksum="8D4CEBB390D5C6E2" modified="2018-07-18" version="1">MGEQTIAGYLGNKSDMKVHHLAAMIPDCEIYYIKKEDRTYFVPDVLEQAIKEKFTPCKHCIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSB7</accession>
    <name>A0A2S2KSB7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Threonine--tRNA ligase</fullName>
            <ecNumber evidence="13">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Threonyl-tRNA synthetase</fullName>
            <shortName evidence="13">ThrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">thrS</name>
        <name evidence="15" type="ORF">NZNM25_13050</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NM25</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="13"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877131.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSB7"/>
    <dbReference type="GeneID" id="76209194"/>
    <dbReference type="OrthoDB" id="372136at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00860">
        <property type="entry name" value="ThrRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000076">
        <property type="entry name" value="Threonine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000001">
        <property type="entry name" value="Threonine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.80.10">
        <property type="entry name" value="D-tyrosyl-tRNA(Tyr) deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023509">
        <property type="entry name" value="DTD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015011">
        <property type="entry name" value="Threonyl-tRNA_syn_edit_dom_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047246">
        <property type="entry name" value="ThrRS_anticodon"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003068">
        <property type="entry name" value="PRK03991.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF44">
        <property type="entry name" value="THREONINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08915">
        <property type="entry name" value="tRNA-Thr_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 13 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 13" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="7 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="14">
        <location>
            <begin position="197"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GBH34514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="620" mass="71245" checksum="1979461F687DD3E5" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSC5</accession>
    <name>A0A2S2KSC5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ketol-acid reductoisomerase (NADP(+))</fullName>
            <shortName evidence="11">KARI</shortName>
            <ecNumber evidence="11">1.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Acetohydroxy-acid isomeroreductase</fullName>
            <shortName evidence="11">AHIR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Alpha-keto-beta-hydroxylacyl reductoisomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ilvC</name>
        <name evidence="15" type="ORF">NZNM25_13710</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate + NAD(+) = (2S)-2-acetolactate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30627"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="1.1.1.383"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-acetolactate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22068"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="1.1.1.383"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-acetolactate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22068"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-ethyl-2-hydroxy-3-oxobutanoate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13493"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49256"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11 12">Belongs to the ketol-acid reductoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.86" evidence="11"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829085.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSC5"/>
    <dbReference type="GeneID" id="76209128"/>
    <dbReference type="OrthoDB" id="6064at2157"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00056"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004455">
        <property type="term" value="F:ketol-acid reductoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000023">
        <property type="entry name" value="Ketol-acid reductoisomerase (NADP(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00435">
        <property type="entry name" value="IlvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013023">
        <property type="entry name" value="KARI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000506">
        <property type="entry name" value="KARI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013116">
        <property type="entry name" value="KARI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014359">
        <property type="entry name" value="KARI_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00465">
        <property type="entry name" value="ilvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004017">
        <property type="entry name" value="PRK05479.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371">
        <property type="entry name" value="KETOL-ACID REDUCTOISOMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371:SF1">
        <property type="entry name" value="KETOL-ACID REDUCTOISOMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01450">
        <property type="entry name" value="KARI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07991">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000116">
        <property type="entry name" value="IlvC_gammaproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51851">
        <property type="entry name" value="KARI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51850">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 11 12" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 11 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="7 11 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KARI N-terminal Rossmann" evidence="13">
        <location>
            <begin position="2"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="KARI C-terminal knotted" evidence="14">
        <location>
            <begin position="183"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="25"/>
            <end position="28"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GBH34580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="332" mass="36933" checksum="A3AD0D5C1B4155D4" modified="2018-07-18" version="1">MAKTWKDNEISLDPIKDQTIAVIGYGIQGDAQANNMKDSGLNVIIGLKEGGNSWKKAEADGHKVMSVAEATKQGDIIHILIPDMIQGQVYKDEIGPNLSEGKALSFSHAAAIYWKWIEAPNNVDLIMVAPKGPGSKVRETYLDNFGTPAIVAVEQDFTGKAWDRTLGIAKAIGSARAGLIKTAFKEEVETDWFGEQADLCGGAASMVTNAFETLVEAGYQPEIAYFEVLHELKLIVDMIQRYGINGMWRRVSETARYGGLTRGPMVMDSANKSNMKKVLTMIQDGTFNNEWISEYQKNGKDAFDKYMKQYDEHQIEKVGKEMRKMMWPDSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSG3</accession>
    <name>A0A2S2KSG3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF726 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">NZNM25_13770</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877191.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSG3"/>
    <dbReference type="GeneID" id="76209122"/>
    <dbReference type="OrthoDB" id="11236at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007941">
        <property type="entry name" value="DUF726"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17920:SF3">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17920">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4 TMCO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05277">
        <property type="entry name" value="DUF726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="27877" checksum="9E08BE5671D139F1" modified="2018-07-18" version="1">MTPIPRISTRGYYDLSNGKTLKTNPYYLYPKKDFKKLTNSKELTIMIHGLRNDRAGAVAKVLLAKNRLRKLGYSHPVVGFSYDSNTTGAHLVKHAKHALAVGQSIAKKNGRNLGLFIEDFKSSSPDTRIRLMGHSLGSQVILSTLEYLSKKKQNIGIIEAVYFFGASITEDIPSSKKFGKILQSIVNKKIINHFAPSDEVLIWADNEKYVKGCLGLNGAIGNPVSKYHQKLVKPKNHRFASYAEVLKSFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSH5</accession>
    <name>A0A2S2KSH5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tryptophan synthase beta chain</fullName>
            <ecNumber evidence="12">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">trpB</name>
        <name evidence="14" type="ORF">NZNM25_12920</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 12">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the TrpB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="12"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877120.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSH5"/>
    <dbReference type="GeneID" id="76209208"/>
    <dbReference type="OrthoDB" id="371827at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06446">
        <property type="entry name" value="Trp-synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000001">
        <property type="entry name" value="Tryptophan synthase beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000004">
        <property type="entry name" value="Tryptophan synthase beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00133">
        <property type="entry name" value="Trp_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006653">
        <property type="entry name" value="Trp_synth_b_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006654">
        <property type="entry name" value="Trp_synth_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00263">
        <property type="entry name" value="trpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077:SF3">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001413">
        <property type="entry name" value="Trp_syn_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00168">
        <property type="entry name" value="TRP_SYNTHASE_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 12" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="13">
        <location>
            <begin position="53"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="12">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GBH34501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="399" mass="43803" checksum="7FF86F9D10CD9BF8" modified="2018-07-18" version="1">MKYPKNGKFGEFGGKYIPETLVPAIEELEENYLKFKNDKNFKKELDYYLKVYAGRPTPLYYAKNLSEKLGGAKIYLKREDLLHGGAHKINNTLGQALLAKKMNKKRIIAETGAGQHGVATAMACAALGMNAEVYMGYKDTIRQKLNVFRMDLLGAKVHPVKSGSKTLKDAINEAIRDWITNVETTYYLLGSAVGPHPYPVMVRDFQSVIGTEIKTQMKKISNKTPDSVIACVGGGSNAIGTFYPLVDTNAEIIGVEAAGHGLKSKMHSATLSAGSKGVLHGMMTYLLQDEEGQITETHSISAGLDYPGVGPEHSYYKDTKRIKYHSATDVEVIDAFLTLTRTEGIIPALESAHAIAEAIKVARKGKRSESIVVTLSGRGDKDVEEVQKYLNKNKKTHTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSK3</accession>
    <name>A0A2S2KSK3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SDR family NAD(P)-dependent oxidoreductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_14510</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877254.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSK3"/>
    <dbReference type="GeneID" id="76209044"/>
    <dbReference type="OrthoDB" id="7442at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF133">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="589" mass="64400" checksum="D0BE3B7461076558" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSL0</accession>
    <name>A0A2S2KSL0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B</fullName>
            <shortName evidence="11">Asp/Glu-ADT subunit B</shortName>
            <ecNumber evidence="11">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">gatB</name>
        <name evidence="13" type="ORF">NZNM25_12290</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 11">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>L-aspartyl-tRNA(Asn) + L-glutamine + ATP + H2O = L-asparaginyl-tRNA(Asn) + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2 11">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the GatB/GatE family. GatB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="11"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877076.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSL0"/>
    <dbReference type="GeneID" id="76209275"/>
    <dbReference type="OrthoDB" id="52755at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.380">
        <property type="entry name" value="GatB domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00121">
        <property type="entry name" value="GatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004413">
        <property type="entry name" value="GatB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042114">
        <property type="entry name" value="GatB_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00133">
        <property type="entry name" value="gatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004012">
        <property type="entry name" value="PRK05477.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004014">
        <property type="entry name" value="PRK05477.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="GLUTAMYL-TRNA GLN AMIDOTRANSFERASE SUBUNIT B MITOCHONDRIAL AND PROKARYOTIC PET112-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659:SF0">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="GatB/YqeY motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Asn/Gln amidotransferase" evidence="12">
        <location>
            <begin position="320"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GBH34438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="467" mass="52517" checksum="91BBBCC53383CDF9" modified="2018-07-18" version="1">MIGLEIHCQLTNLQSKLFCSCKANYREFEINENICPICIGLPGSLPRLNKEAIKKATTIAMALNCTTPEKIAFFRKNYFYPDLPKNFQITQLNIYGDTSVGGHGSVTVGNKKIRITRIQLEEDPGRLVYEGSSSKNMITLVDYNRAGTPLVEIVTEPDFETPKEVRDFLNILSDLLENLEVSDPSLEGAMRADANVSIKGGKKVEIKNIGSFHDLEKAVHFEITRQESLNSRDIPIIQETRHWDDKRKITVSARSKEEEHDYRYFLEGDIPWIKIDTAILEELKSEMPESIISKKERYVSKYNIPAQVADVLSSDKFYSDLFEKAHTEKNAKEIANIITTDLMGLVDTREKREASKLTSTHLRELADSIQSGKISRNSSKNALQEIVKSGKDLSVIISELDLGNVSDASELIAIIQQVISEEPQAIEQTKINPQAINFLVGKVMQKTKGKADPILTLDLLKKQIDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSN7</accession>
    <name>A0A2S2KSN7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VTT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_14670</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877266.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSN7"/>
    <dbReference type="GeneID" id="76209028"/>
    <dbReference type="OrthoDB" id="10722at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051311">
        <property type="entry name" value="DedA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="VTT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42709">
        <property type="entry name" value="ALKALINE PHOSPHATASE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42709:SF10">
        <property type="entry name" value="SNARE ASSOCIATED GOLGI PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="VTT_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="VTT" evidence="2">
        <location>
            <begin position="35"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="210" mass="23444" checksum="F1BA984288CB49B6" modified="2018-07-18" version="1">MDFVDLFPFAPEVGYLSLSLVNFFGSLIPFIPLPGFLLLATMAVGDQFDLHVLALLSAITATVAKQIIFYVSYGGRKIINEKTRKRMRPFERLVKRYGAGAAFFAAATPIPDDLVYVPLGLAKYNPKRFFIATLTGKVVLSYSIVFISHYLGLSLVDPLIENIDDATPVYIGIIIFGIMMTSVVILLLRLDWEKILGKFAPWTLDENNEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSR1</accession>
    <name>A0A2S2KSR1_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methionine--tRNA ligase</fullName>
            <ecNumber evidence="7">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Methionyl-tRNA synthetase</fullName>
            <shortName evidence="7">MetRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">metG</name>
        <name evidence="11" type="ORF">NZNM25_13780</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="7"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877192.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSR1"/>
    <dbReference type="GeneID" id="76209121"/>
    <dbReference type="OrthoDB" id="371856at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07957">
        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.20">
        <property type="entry name" value="Methionyl-tRNA synthetase, Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00098">
        <property type="entry name" value="Met_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023458">
        <property type="entry name" value="Met-tRNA_ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029038">
        <property type="entry name" value="MetRS_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001100">
        <property type="entry name" value="PRK00133.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765">
        <property type="entry name" value="METHIONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765:SF1">
        <property type="entry name" value="METHIONINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
        <property type="entry name" value="Anticodon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57770">
        <property type="entry name" value="Methionyl-tRNA synthetase (MetRS), Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="3 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 7 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Methionyl/Leucyl tRNA synthetase" evidence="9">
        <location>
            <begin position="6"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl-tRNA synthetase anticodon-binding" evidence="10">
        <location>
            <begin position="404"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="7">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="7">
        <location>
            <begin position="328"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF19303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="552" mass="63459" checksum="7CCB87BD76DE6B5F" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSS1</accession>
    <name>A0A2S2KSS1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cobalamin-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_13880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSS1"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01144">
        <property type="entry name" value="BtuF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051030">
        <property type="entry name" value="Vitamin_B12-ABC_binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42860">
        <property type="entry name" value="VITAMIN B12-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42860:SF1">
        <property type="entry name" value="VITAMIN B12-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53807">
        <property type="entry name" value="Helical backbone' metal receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="1">
        <location>
            <begin position="1"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="301" mass="34232" checksum="F1F74BADE933EC9E" modified="2018-07-18" version="1">MPSATELLYEFGVQNDLFGVTHECKFPQDATTKPRIINSVINSDELTSDQINTMTCRLLNDGKDIFTVDEEKIREADPDLIIAQETCEVCAAHTNQVNKALQILQTKPILYSMDPHNLQEIIQSVIELGKILGKESKAREITESLEKRIDGIRNSPKNKKPKVLAIEWIEPFFTAGHWIPEMIEIAGGVNTISKMGEHSHRLEFSEIVKADPDIIIMMPCGFDTKRTILEYNNILKKDQKWNSLRAVKENKVFAVDANSFFSKPSIRTIEGLEILAKVIQPKKFKNLKVSDESFFRIKNES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSS9</accession>
    <name>A0A2S2KSS9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoribosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_13980</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877210.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSS9"/>
    <dbReference type="GeneID" id="76209101"/>
    <dbReference type="OrthoDB" id="4952at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363">
        <property type="entry name" value="HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363:SF2">
        <property type="entry name" value="PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="136" mass="15521" checksum="B1E2B8BE5290B1CA" modified="2018-07-18" version="1">MTLKQNVDWNEIESLVNILSKKITKLSRTFSTITTIGRGGLVPSRLLADRLGIEKILVDKNKISSDSLFVDDIFDTGDTFTNIIPKVDNPSKLVFATLFARRGKKYPSQLIYAKKTDNSAYVVFPWDRFEFKRSQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSU6</accession>
    <name>A0A2S2KSU6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Translation-associated GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ychF_2</name>
        <name evidence="5" type="ORF">NZNM25_15270</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RelA/SpoT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSU6"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000002">
        <property type="entry name" value="GTP pyrophosphokinase (RelA/SpoT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.470:FF:000001">
        <property type="entry name" value="GTP-binding protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013646">
        <property type="entry name" value="YGR210-like_G4"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007171">
        <property type="entry name" value="PRK09602.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF1">
        <property type="entry name" value="OBG-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08438">
        <property type="entry name" value="YGR210-like_G4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="4">
        <location>
            <begin position="1"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="355" mass="39116" checksum="B605D00A35E0E112" modified="2018-07-18" version="1">MAYVKADCACKHFEIKHENEFCVNGIRFIPVKLIDVAGLVPGAHEGKGLGNQFLDDARQAEVLIHVVDIAGTTDIQGQPVPAGTHDPLEDIAFVQDEFDQWFADILKREWDKITREIDQKRAKLVDGIAKRFSGLGVKDFQVQEVLQKLGFMAKNPKEWNDSDIQNFAKELRKNTKPLIIAANKADLCKDLGVLEKISDCVVPCSAETELLLRKASKAGIVNYSSGDANFTIVHGKEIPPPQQKALDLVKSVFSKIPSTGIQKILNTAVFDLLKFIVVYPVEDETKLTNKDGVILPDAKLLPLDSTAKDLASLIHADIAKGFLHAIDCKTKQRISGDQKLKNGDVIKIVSTLSRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSV5</accession>
    <name>A0A2S2KSV5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_14320</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877238.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSV5"/>
    <dbReference type="GeneID" id="76209063"/>
    <dbReference type="OrthoDB" id="3281at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="287" mass="33339" checksum="B62D8055A5313B31" modified="2018-07-18" version="1">MFHFVLFGLLTLGGITFDDNLISTLTDLENITSQFGTDVPVTDLIGYSIGMVIYGIFIWNFYRFLARREMIPLRLKKYQTDGKKITSIIAYVFKYVIIFPLVILVWFVVYSTFLFFMAPDLPTEHVFLIVISLVVTVRISAYYKEDLAKDFAKLIPFALLGIFLTSNIFFTPNDILDRAYGFVPFLGKILGFVIYAILVESILRILFLTKRKILPVAEEKLAEQIEEQIDEKIKVHVEKIEEKQKNLEQKIEKETDEIEKKMEKESNEIKKKLDKENTTSNKDSIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSV6</accession>
    <name>A0A2S2KSV6_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Asparagine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_13190</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSV6"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050795">
        <property type="entry name" value="Asn_Synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772">
        <property type="entry name" value="ASPARAGINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772:SF46">
        <property type="entry name" value="ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Asparagine synthetase" evidence="3">
        <location>
            <begin position="25"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Asparagine synthetase" evidence="3">
        <location>
            <begin position="170"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="314" mass="35879" checksum="E3D69CE0CF89C92B" modified="2018-07-18" version="1">MLDNVSKELYDVLEESCNSCKSNLIALSGGLDSSIIAYFLRQKKPRTVAVIAEDFVSTDLTYCQRVAKELGLSLTIYYVKTAEILEAVEDTVKILKNFNDIEIRNNVVMYLAIKWAKDNGEKSIITGDGADELFAGYSFLIHKPENELEAEISRVCSVMHFPTQKIGKELGIEVEAPFLKDTVIELAKKIPADMKVRNEKEQRYGKWILRKTFEKYLPQQIIWREKSPMQDGSGTAGLTNLFESIINEEKYVEKKLTTEKEDGVIIRSRESMHYYEIFKRLFGVPQKSPDNACPYCKCEVKNSKFCRMCGAFPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT30</accession>
    <name>A0A2S2KT30_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptide methionine sulfoxide reductase MsrA</fullName>
            <shortName evidence="2">Protein-methionine-S-oxide reductase</shortName>
            <ecNumber evidence="2">1.8.4.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Peptide-methionine (S)-S-oxide reductase</fullName>
            <shortName evidence="2">Peptide Met(O) reductase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">msrA</name>
        <name evidence="4" type="ORF">NZNM25_14060</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(S)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:14217"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12315"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:44120"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>[thioredoxin]-disulfide + L-methionine + H2O = L-methionine (S)-S-oxide + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:19993"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58772"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MsrA Met sulfoxide reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.11" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877218.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT30"/>
    <dbReference type="GeneID" id="76209093"/>
    <dbReference type="OrthoDB" id="7150at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033744">
        <property type="term" value="F:L-methionine:thioredoxin-disulfide S-oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008113">
        <property type="term" value="F:peptide-methionine (S)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1060.10">
        <property type="entry name" value="Peptide methionine sulphoxide reductase MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01401">
        <property type="entry name" value="MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036509">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00401">
        <property type="entry name" value="msrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43774">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43774:SF1">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE MSRA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55068">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptide methionine sulphoxide reductase MsrA" evidence="3">
        <location>
            <begin position="2"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="151" mass="17339" checksum="43559AC6A5203FB4" modified="2018-07-18" version="1">MKATFGAGCFWHVEDLFNKTKGIKSTHVGYIGGQLPNPTYEEVCTDQTGHAEAVEVEFDPDEISYEELLDVFWNNHNPTTLNRQGPDVGHQYRSAIFVHNEEQKKIAEKSKQDLEKSGRFENPVVTEIVPAPTFYKAEEYHQKYFKKHGFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT35</accession>
    <name>A0A2S2KT35_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_15320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012215921.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT35"/>
    <dbReference type="GeneID" id="40722082"/>
    <dbReference type="OrthoDB" id="5631at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="70" mass="7942" checksum="5D771FF5E38E41F2" modified="2018-07-18" version="1">MGRSFQEWLGQQDQAVVAKTRAGDEANKPLLNQINWIWVNNLMNQKADLNPSSAELLDWVTSGQIDAMRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT36</accession>
    <name>A0A2S2KT36_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_15080</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877302.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT36"/>
    <dbReference type="GeneID" id="76208988"/>
    <dbReference type="OrthoDB" id="2830at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00156">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050595">
        <property type="entry name" value="Bact_response_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591:SF3">
        <property type="entry name" value="RESPONSE REGULATORY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591">
        <property type="entry name" value="STRESS RESPONSE REGULATOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="7"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="141" mass="15818" checksum="7B3A5C86704DAB4E" modified="2018-07-18" version="1">MARKFPVILLVDDSQAFRVFCRDAIKSSIKFIQVLEASDGIEALKQYQLHRPDVILLDLKMPRLEGDKVLEMIKKNDQNTKIIATSAYGRDQESINKLLKMGAISFVPKPLNRIGLMKEITDVLAKGKMPGTNFTKSIPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT90</accession>
    <name>A0A2S2KT90_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_16400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829103.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT90"/>
    <dbReference type="GeneID" id="76208345"/>
    <dbReference type="OrthoDB" id="3172at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="518" mass="59179" checksum="28BB0ED00F0F5E87" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTA2</accession>
    <name>A0A2S2KTA2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Nickel-responsive regulator 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_16500</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transcriptional regulatory CopG/NikR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877476.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTA2"/>
    <dbReference type="GeneID" id="76208335"/>
    <dbReference type="OrthoDB" id="9459at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22231">
        <property type="entry name" value="RHH_NikR_HicB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="entry name" value="ACT-like. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="entry name" value="Met repressor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050192">
        <property type="entry name" value="CopG/NikR_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014864">
        <property type="entry name" value="TF_NikR_Ni-bd_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34719">
        <property type="entry name" value="NICKEL-RESPONSIVE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34719:SF3">
        <property type="entry name" value="NICKEL-RESPONSIVE REGULATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08753">
        <property type="entry name" value="NikR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Ribbon-helix-helix protein CopG" evidence="5">
        <location>
            <begin position="4"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor NikR nickel binding C-terminal" evidence="6">
        <location>
            <begin position="60"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH34859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="127" mass="14196" checksum="03ED18553ACAF80F" modified="2018-07-18" version="1">MPIVSISLNDEILSELDKLQTTMGFSGRSEAIRAGIRSFVSEEKQKADLSGNIHAILLVVHNDEFDHVVSGITHNFEDLITTHLHSKIEKEKCMELFLINGDAEKVSTITKDFQTNKNMDTVKLVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTD9</accession>
    <name>A0A2S2KTD9_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA 3'-terminal phosphate cyclase</fullName>
            <ecNumber evidence="1">6.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_17030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.4" evidence="1"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877521.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTD9"/>
    <dbReference type="GeneID" id="76208283"/>
    <dbReference type="OrthoDB" id="7994at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003963">
        <property type="term" value="F:RNA-3'-phosphate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023797">
        <property type="entry name" value="RNA3'_phos_cyclase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037136">
        <property type="entry name" value="RNA3'_phos_cyclase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017770">
        <property type="entry name" value="RNA3'_term_phos_cyc_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020719">
        <property type="entry name" value="RNA3'_term_phos_cycl-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036553">
        <property type="entry name" value="RPTC_insert"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03399">
        <property type="entry name" value="RNA_3prim_cycl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="RNA 3' TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF0">
        <property type="entry name" value="RNA 3'-TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01137">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005378">
        <property type="entry name" value="RNA3'_term_phos_cycl_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01287">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase" evidence="2">
        <location>
            <begin position="11"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="NCBIfam" id="TIGR03399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="341" mass="37890" checksum="287417B5796B01FC" modified="2018-07-18" version="1">MNFLKINGEFGEGGGQIIRSAITLACITKQSIHLENIRKNRKVPGLRPQHLTAVKILQKISNAKVIGAEIGSTELKFIPRDLDNLELSEDVGTAGSITLILQVLIPVVAISQKKLSLTIKGGTDVPWSPSFEYTQHILKEAYSRMGIEFSLQLIKRGYYPKGGGEVKLQVYPSRLKSILFSKRKTNNVKLICTFSKIPIEIIRNEIKRIKKKLSDENFNVETETKNEDAIDSGASLLIYSIDENSIIGVDSLFNKKTQSFDLDFDKFINNLQSVDENLADMLVVPASLGPRKTTFLIKDITKHLETNLFVTSKITGCKYGVGKLTNGFEVIVEGISNSSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTE4</accession>
    <name>A0A2S2KTE4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator TrmB N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_15190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877313.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTE4"/>
    <dbReference type="GeneID" id="76208977"/>
    <dbReference type="OrthoDB" id="30795at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051797">
        <property type="entry name" value="TrmB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="1">
        <location>
            <begin position="13"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="269" mass="30120" checksum="33E31E8BBDEEB690" modified="2018-07-18" version="1">MLSKDEIYNSLVHFGLEEIDAKIYVGLLQMGSVTVGTMAQKLNVDRGKAYRSLNKLRNMGVISTTFSNPTIVNAVEPSEALTSVIQKKEDEIVMLQKLARTVIESLHNYENNVAATDLSSFSIVQGRSNIYTRIGKLIQESTDKIFLVTTSDDLMRMYHTSIPDKIHAKISAGGEVRIITNDCDSKTMEIIEQLGTPEVKIGKLPSKSRMIVESKHQLIMSGAMKESMDLNDDVDSIMYTNSEEMVENMFSLCTHLWKKSKPMEILSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTE6</accession>
    <name>A0A2S2KTE6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_16420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTE6"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="520" mass="57984" checksum="889A69D3B38B5B56" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTI8</accession>
    <name>A0A2S2KTI8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Dephospho-CoA kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_17550</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829112.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTI8"/>
    <dbReference type="GeneID" id="76208230"/>
    <dbReference type="OrthoDB" id="85381at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41930:SF1">
        <property type="entry name" value="DEPHOSPHO-COA KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41930">
        <property type="entry name" value="UPF0200 PROTEIN MJ1399"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13207">
        <property type="entry name" value="AAA_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="182" mass="20080" checksum="9C26DAB18D8C4ACA" modified="2018-07-18" version="1">MAKLIVCLTGMPGAGKSTIAEGLKSKGYDIINMGNAVREEAKKRNLELTRANLGKLMLELREQNGPGAVAELVKPQIESSTSNVILIDGVRSNDEIQVLKKFGTVKLLAIHASTDTRFDFLQKRGRSDDPQTKEHFDERDNRELGVGISNSIALSDYAISNIGLTKDELIHIAYEIIQSWIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTJ4</accession>
    <name>A0A2S2KTJ4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_15690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829098.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTJ4"/>
    <dbReference type="GeneID" id="76208418"/>
    <dbReference type="OrthoDB" id="1587at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="121" mass="14047" checksum="78AF637259840D94" modified="2018-07-18" version="1">MEYEKICDEVLECDKKIRYVGIYDYGELYDKMRQGLKSHLTREETEMSLSQAVYRWSTRKKTSDKIGKPIFALAKYEKIYRITIPIGGAGLILISTELDVDVNKTVDKILEIKNKYSKNLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTL3</accession>
    <name>A0A2S2KTL3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 5">Adenylyl-sulfate kinase</fullName>
            <ecNumber evidence="1 5">2.7.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cysC</name>
        <name evidence="7" type="ORF">NZNM25_18010</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the synthesis of activated sulfate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24152"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the APS kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.25" evidence="1 5"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTL3"/>
    <dbReference type="UniPathway" id="UPA00140">
        <property type="reaction ID" value="UER00205"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004020">
        <property type="term" value="F:adenylylsulfate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004781">
        <property type="term" value="F:sulfate adenylyltransferase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070814">
        <property type="term" value="P:hydrogen sulfide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010134">
        <property type="term" value="P:sulfate assimilation via adenylyl sulfate reduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019379">
        <property type="term" value="P:sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02027">
        <property type="entry name" value="APSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002891">
        <property type="entry name" value="APS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059117">
        <property type="entry name" value="APS_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050512">
        <property type="entry name" value="Sulf_AdTrans/APS_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00455">
        <property type="entry name" value="apsK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42700">
        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42700:SF1">
        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01583">
        <property type="entry name" value="APS_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="APS kinase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH35010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="164" mass="18278" checksum="3941C8B387AA10FA" modified="2018-07-18" version="1">MTGLPCSGKTTIVKDLQKDIPNLAMLDGDELREWFSPKDFSKAGRDEHNKKVAHLAKLLLNHGVPSVVSLVSPYSENRKNAREIINAGNQFAEVYVKCSLAKCEERDVKGMYAKARKGEIKGFTGIDDPYEAPEKADLVIDTENEPLSDSANKVKDFLKERNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTM6</accession>
    <name>A0A2S2KTM6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">GTP cyclohydrolase III</fullName>
            <ecNumber evidence="2 3">3.5.4.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">gch3</name>
        <name evidence="5" type="ORF">NZNM25_16090</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 3">Catalyzes the formation of 2-amino-5-formylamino-6-ribofuranosylamino-4(3H)-pyrimidinone ribonucleotide monophosphate and inorganic phosphate from GTP. Also has an independent pyrophosphate phosphohydrolase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>GTP + 3 H2O = 2-amino-5-formylamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + 2 phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:22468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57258"/>
            <dbReference type="EC" id="3.5.4.29"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the archaeal-type GTP cyclohydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.29" evidence="2 3"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877446.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTM6"/>
    <dbReference type="GeneID" id="76208377"/>
    <dbReference type="OrthoDB" id="25211at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043740">
        <property type="term" value="F:GTP cyclohydrolase IIa activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00608">
        <property type="entry name" value="GTP_cyclohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007839">
        <property type="entry name" value="GTP_CycHdrlase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42202">
        <property type="entry name" value="GTP CYCLOHYDROLASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42202:SF1">
        <property type="entry name" value="GTP CYCLOHYDROLASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05165">
        <property type="entry name" value="GCH_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009265">
        <property type="entry name" value="GTP_cyclohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="4">
        <location>
            <begin position="127"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR009265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GBH34818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="250" mass="28494" checksum="0E1993EC29D3FE89" modified="2018-07-18" version="1">MIQLSILKITGYGPWTLTLGSDREHELQMLQASLYKEVQKLFSEKNCLVFLNRADEFFVITNGLKLEDHIEIQKKLENLFDIRLAISIGYGESPFDANLKAYDGKKNNIILNQEHNIFGFVKNVPDSKVSIMHLDVDDLTSRRKNNSPYEITSIIFELYSKMSKYFLAKNSLTFFMGGDNFMIIASEDGKNSVQNFINMIKDNDNISLNCGIGNAYTSREAAKLATKSLDTIREIRDSGKEKPEVYELSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTN8</accession>
    <name>A0A2S2KTN8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_16190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877455.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTN8"/>
    <dbReference type="GeneID" id="76208367"/>
    <dbReference type="OrthoDB" id="8709at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08266">
        <property type="entry name" value="Zn_ADH_like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051603">
        <property type="entry name" value="Zinc-ADH_QOR/CCCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44154">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44154:SF1">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="2">
        <location>
            <begin position="17"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="359" mass="38825" checksum="80F88C24939EEF68" modified="2018-07-18" version="1">MKAVVYNEYAPDDNFSKILKVQDIDDPKPKADEVVFKVKAAALNYNDIWGMRGVPVAVPLPHVSGSDAAGDVIAVGEDVKNIKVGDRVVSHSNMSCRVCKACTDGREFDCINRTIWGFQTGPTWGAFQEVTHLPEVNVSVIPEGVSYDDAAAASMTLLTSWHMLVGRAKIVPGQTVLIMGGGSGVGSFGIQIAKLYNCDVIATASPDKLDKCLELGADFAVDHRKEDWHKEVRAISKELAKKKGEAPGIDVSFDHIGETHWNKQLTLLKYGATLVSCGATTGYDAQTDLRHIFFKGTNILGSTQGTKAELDQGLYWMGQGKIKAAIDSTYSFEQAAEAHTKMLTGKGLFGKILMKPEGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTS0</accession>
    <name>A0A2S2KTS0_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mechanosensitive ion channel protein MscS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_17480</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877556.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTS0"/>
    <dbReference type="GeneID" id="76208237"/>
    <dbReference type="OrthoDB" id="121853at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049278">
        <property type="entry name" value="MS_channel_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011066">
        <property type="entry name" value="MscS_channel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30566:SF5">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30566">
        <property type="entry name" value="YNAI-RELATED MECHANOSENSITIVE ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21082">
        <property type="entry name" value="MS_channel_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82689">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="302"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="326"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="8">
        <location>
            <begin position="347"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS C-terminal" evidence="9">
        <location>
            <begin position="464"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH34957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="582" mass="66703" checksum="D989F572D19A05D1" modified="2018-07-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTT5</accession>
    <name>A0A2S2KTT5_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphomevalonate dehydratase large subunit</fullName>
            <ecNumber evidence="7">4.2.1.182</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_18300</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of a hydro-lyase that catalyzes the dehydration of mevalonate 5-phosphate (MVA5P) to form trans-anhydromevalonate 5-phosphate (tAHMP). Involved in the archaeal mevalonate (MVA) pathway, which provides fundamental precursors for isoprenoid biosynthesis, such as isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(R)-5-phosphomevalonate = (2E)-3-methyl-5-phosphooxypent-2-enoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:78975"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:229665"/>
            <dbReference type="EC" id="4.2.1.182"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:78976"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer composed of a large subunit (PMDh-L) and a small subunit (PMDh-S).</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the AcnX type II large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.182" evidence="7"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877629.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTT5"/>
    <dbReference type="GeneID" id="76210291"/>
    <dbReference type="OrthoDB" id="25253at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007506">
        <property type="entry name" value="PMDh-L-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36577">
        <property type="entry name" value="DUF521 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G00490)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36577:SF3">
        <property type="entry name" value="DUF521 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G00490)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04412">
        <property type="entry name" value="AcnX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphomevalonate dehydratase large subunit-like" evidence="9">
        <location>
            <begin position="1"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00045120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00045299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH35039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="384" mass="42180" checksum="D426FE59A54F89AF" modified="2018-07-18" version="1">MELTREEESALKGEQGEIMQMAYRILIATGEATDAEKLIPIEWAHLSGVNYNTIGDAGEEFLSSISKDARVTVKTTLNPMGFDIDNVINYGLDENFILKQLSIRKSYETMGVIPSFSCIPYEIFEIPKDGTQVAFAESNAAIHANSYDNLKTNKESAFSALASALVGKSPYSSIRKDDTPNVTINMKIKNPNELTYGMLGFFAGKVGDTSVNISGLAEMDNRQCKAMCGGMGTSGTCAKFIFSDGDSDCEKIDFDEKEMQNVHDELNTAEKGDMITLGSPQLGLDEISDLTNMLKGRSFQKRCMVFCPRTVKEQARKIGYTTELERAGCEILSDCCTCLTPLINKDNVDSVTTNSIKGAFYLKNSNGVDVNLKSLSQIIQDETR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU17</accession>
    <name>A0A2S2KU17_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Holo-[acyl-carrier-protein] synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">acpS</name>
        <name evidence="9" type="ORF">NZNM25_19510</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877761.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU17"/>
    <dbReference type="GeneID" id="76210181"/>
    <dbReference type="OrthoDB" id="186883at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="entry name" value="4'-phosphopantetheinyl transferase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00101">
        <property type="entry name" value="AcpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002582">
        <property type="entry name" value="ACPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004568">
        <property type="entry name" value="Ppantetheine-prot_Trfase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00556">
        <property type="entry name" value="pantethn_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="4'-phosphopantetheinyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="4'-phosphopantetheinyl transferase" evidence="8">
        <location>
            <begin position="6"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH35160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="119" mass="13654" checksum="9918C35C26818620" modified="2018-07-18" version="1">MLENIGIGIDIIEIERFKEKPYQDNEIFYKKIFHLSEIDYCLNNKNSSQSFAVKFAIKESVIKSINKKIDFLDIITDHLDSKPIVSLSKDPSYNFLVSVSHEKQNAIAVVISKKNSLNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU52</accession>
    <name>A0A2S2KU52_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_19370</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877749.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU52"/>
    <dbReference type="GeneID" id="76210167"/>
    <dbReference type="OrthoDB" id="3125at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20489">
        <property type="entry name" value="cupin_HppE-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050807">
        <property type="entry name" value="TransReg_Diox_bact_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46797">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46797:SF1">
        <property type="entry name" value="METHYLPHOSPHONATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13443">
        <property type="entry name" value="HTH_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="2">
        <location>
            <begin position="246"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH35146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="456" mass="52039" checksum="2075BA2921F7147C" modified="2018-07-18" version="1">MQKEESIINLHQIKSGKNFLGILNDIKRRPEDAANELNVTLEEIQAIIEGTKPLTPELINKAIEIWPINARDFYIVQDDCPLGIKIMRADESQKSSRIMNRAGRPYYEYRDTAMSTVAPFRPEWILELCYVDDNEPTNLDVQWNNGHFMHQFTYFIGDVNFYYKDESGNKKVAIMNTGDSMYITPFTPHTFTSRKGCKENGLILALTYGGKLTGDVQQELSGLSVELGSKFALDFSTKESASSSLLKFHREISNISIEELSKRTGLDVDKIKNFENKLEFPQMSDLQKIANALTINIRDLLPNDKTENKVIVKYHDKCRKWLYPENSKAYEFIELAGTSALPFSKAFEVIIRNSENSEYDLQSGLHQYVYNVGDTTITVNWKYGEKNYSERINPGDSIYGKPFISHNFRGNGKLLILRIGGKIAGDSQRELSIVGKKNAQRAISETMQWFDPQGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU77</accession>
    <name>A0A2S2KU77_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_19830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829120.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU77"/>
    <dbReference type="GeneID" id="76210213"/>
    <dbReference type="OrthoDB" id="5657at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="54" mass="6136" checksum="B2E3F8A43B4C7A68" modified="2018-07-18" version="1">MACFCGCETYEKYEDGFKIACVKCGHGPQNHDEEFRAAARKNESQSQKRDVDFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU85</accession>
    <name>A0A2S2KU85_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">YbhB/YbcL family Raf kinase inhibitor-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_20210</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877835.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU85"/>
    <dbReference type="GeneID" id="76210255"/>
    <dbReference type="OrthoDB" id="28720at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00865">
        <property type="entry name" value="PEBP_bact_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005247">
        <property type="entry name" value="YbhB_YbcL/LppC-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00481">
        <property type="entry name" value="YbhB/YbcL family Raf kinase inhibitor-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30289:SF1">
        <property type="entry name" value="PEBP (PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30289">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YBCL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="16457" checksum="1779674FC174835B" modified="2018-07-18" version="1">MIITSPAFKNGQSIPRKYGYKNENSSPPLLIDKIPEGTESLALIMDDPDAMKAVGKVWVHWVLWNIPSNTQEIKENSIPEGSVEGKTDFGEIGYGGPAPPDKEHLYIFKLYALDTKLDLGQNSIKTDLEESMKNHILAEAKLEGRYAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUA4</accession>
    <name>A0A2S2KUA4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_19280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877741.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUA4"/>
    <dbReference type="GeneID" id="76210158"/>
    <dbReference type="OrthoDB" id="5019at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="62" mass="7084" checksum="E03C488C7A153C85" modified="2018-07-18" version="1">MKTNFRKNESTPSQSEDSNEDSGKKDLTQKEFEEYIAKSGAKGSFMVYRSTKKDEELGLDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUE2</accession>
    <name>A0A2S2KUE2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_18860</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_160049227.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUE2"/>
    <dbReference type="GeneID" id="76210113"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="49" mass="5978" checksum="3EECD1EC53E584C8" modified="2018-07-18" version="1">MNEHSYFASLKNKEFLILEKLCDESISPTEKERMEKEIRHIRLEIKKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUH8</accession>
    <name>A0A2S2KUH8_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Formyl transferase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_19260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877739.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUH8"/>
    <dbReference type="GeneID" id="76210156"/>
    <dbReference type="OrthoDB" id="199806at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08653">
        <property type="entry name" value="FMT_core_like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Formyl transferase N-terminal" evidence="1">
        <location>
            <begin position="79"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="258" mass="29733" checksum="A0C91BA89D36224B" modified="2018-07-18" version="1">MKIVILTSNGLRHKFLANSLASKADDTLIISECKQNDSVSTGLEDQSTISEHFRLRNETEKIFFPNNDFFTAKTLPILYKEVNLEYVYQTIKKFQPDVMFIFGSYIIREPLLSLMPPDRTINLHLGLSPYYRGSGTNFWPFVNNELEYVGSTILHLDSGIDTGDIICHTRPKIKLGDNVHTVGCKVIQSSVDSLIQIMEMIKEGKKLNRIKQWSIDEKYYKTIDFNEEILLKYKNNLENGLIENYLSKPKKSIKLIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUM3</accession>
    <name>A0A2S2KUM3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Iduronate-2-sulfatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_19390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877751.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUM3"/>
    <dbReference type="GeneID" id="76210169"/>
    <dbReference type="OrthoDB" id="3164at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004065">
        <property type="term" value="F:arylsulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015024">
        <property type="term" value="F:glucuronate-2-sulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051849">
        <property type="entry name" value="GAG-degrading_sulfatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46615">
        <property type="entry name" value="ARYLSULFATASE K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46615:SF1">
        <property type="entry name" value="ARYLSULFATASE K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfatase N-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="449" mass="51384" checksum="17691F4E9C60468C" modified="2018-07-18" version="1">MKPNILLLTIDSLRADKIYGKNKSSLTPNIDNLIKNGLCFTQAISTSDTTGLSIGSLVTAKYPFKTGITHFSYDNNTQTYFQILKDNGYKIYATIPDSSFFLKMTANMTETDAYVYDKRESWLQLVGGIGEQIVKRLEKNLTEPWFYYIHLMDLHAPFYVPKEFDSEKFGKTPYDRMISAIDSWIGKFFEKIDLKKTLLIISADHGDHIPVVDDWGKIPKVNILLKKGKQKLPILEPLGLRLFVAYQSLKRKYKSAKLSKELSDRELIALEGRGQNHLFDELIRIPLIFTGFGINYPKIITNQVKQVDIFPTIVDLVEISSNIKNIDGQSLVPLISNPSQKDSYTYIETGARNFKNAKNPTLHGNIIGLRTPKYKYWRSRNDSTKNVTLYDLENDPNEENNIAEKNQSVVISMEEIIVNMKKNSIPIKYEISKDDEGAIEEELKKMGYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KUS5</accession>
    <name>A0A2S2KUS5_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Metalloprotease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_19890</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KUS5"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031293">
        <property type="term" value="P:membrane protein intracellular domain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001193">
        <property type="entry name" value="MBTPS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13325:SF3">
        <property type="entry name" value="MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE-2 PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13325">
        <property type="entry name" value="PROTEASE M50 MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE 2 PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01000">
        <property type="entry name" value="SREBPS2PTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="153"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="373"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="416"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="241"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH35198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="445" mass="49281" checksum="EB56C094DF49604F" modified="2018-07-18" version="1">MTHATVLFFTSSFAIFEIDFLSGYVETIITGFIVTDKCTFFKKLTISLELDFITQNSIIYVLIAWVVIVIIAKALKVEKYGFEIKAYSLVYKNKGVNVVLNKVLSRTKRGIRVFADTSVIAGFVMMGFAFWFLLNNVSNFFVAQDEFSELTVLIPGITLTSASSIAFFLLSIPIVLVVHEGAHGIVAALEKIKIKTGGFAIFIAMFAGFVEPDEEEFEKAKKISKLRVIGAGATANVIFAFGLGLILLTNPFFAMVLPEPLLTIFYELPSGVLILSIIENSGAEQAGLLANDIITSINGIQIYSPADFPSLSPGETASVSVIRDGQELDFGVEIIPSPEDPERGLIGIMRDNTFAYKPLMNFIEWNDPNVSMFLLWLWMISFFIGIINMLPLPILDGGKFIHTIIDKKISDKSVNIVMWGIYAFTFVLFGLNIALSYMKSGWFTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KNN7</accession>
    <name>A0A2S2KNN7_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_00410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109875924.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KNN7"/>
    <dbReference type="GeneID" id="76209371"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="127" mass="13679" checksum="5D1A425A6D385CF2" modified="2018-07-18" version="1">MAISKAKRAEAAKKAARTRKRNAAKAAAEALKAAAARKRKAAATRRKNAGKAAPKRKAAAKRRAAPKRKAAPKRRTAAKKAAPKRKAAAKRRAAPKRRTAAKKAAPKRKAAAKRRAAPKRKAAKRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP71</accession>
    <name>A0A2S2KP71_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MFS transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_01580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876027.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP71"/>
    <dbReference type="GeneID" id="76209488"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="73" mass="8158" checksum="A1F935F17929065A" modified="2018-07-18" version="1">MSQNPIDPPEQIILVITTAIIDIVFSILFNEINSYASESPQALGLMIMVYGAVVTLNGLTTWFVILPLFERHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KP95</accession>
    <name>A0A2S2KP95_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Restriction endonuclease type IV Mrr domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_02670</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_370684778.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KP95"/>
    <dbReference type="GeneID" id="76209602"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="23976" checksum="58211ED8FFD099F5" modified="2018-07-18" version="1">MELKIHPKMIIGLNGIIQGGVSVEDFSAVTKINFVDSEIILEEFVKNGIGTKQNDNYYFDGGDKLKIAVALLENGYPIDEISISLDWKDFEGLTGEILSSKNFAVIKNMILTKPRMEIDVIGIRLGIAILIDCKHWKRYSSSALKTAVKKQIERTKHYVEKTPGGMAVPVIVTLYQDKVNFIENVPIVPIFQLTSFIDEFYGNIDQMKTIETD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KPK8</accession>
    <name>A0A2S2KPK8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA-3-methyladenine glycosylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tag</name>
        <name evidence="2" type="ORF">NZNM25_03250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000001">
        <property type="protein sequence ID" value="GBH33534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KPK8"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008725">
        <property type="term" value="F:DNA-3-methyladenine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005019">
        <property type="entry name" value="Adenine_glyco"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052891">
        <property type="entry name" value="DNA-3mA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30037">
        <property type="entry name" value="DNA-3-METHYLADENINE GLYCOSYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30037:SF4">
        <property type="entry name" value="DNA-3-METHYLADENINE GLYCOSYLASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03352">
        <property type="entry name" value="Adenine_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR605019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="190" mass="22151" checksum="101904B75B19B086" modified="2018-07-18" version="1">MLTLTQIMNRCEWAKDEPNITYHDKEWGKPQHDDQKLFEFLILEGAQAGLSWTTILKRRDGYRKAFSDFNALKVSRYDKKHIEKLLRDESIIRNKLKINSAINNAKQFLKIQIEFGSFDNYLWGFVNHTPIKNSFKKSSELPSSTSLSKKISLDMKKHGFTFVGPTICYALMQAVGMVNDHTMDCYLFEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQ40</accession>
    <name>A0A2S2KQ40_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PEFG-CTERM sorting domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_05740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876429.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQ40"/>
    <dbReference type="GeneID" id="76209926"/>
    <dbReference type="OrthoDB" id="4997at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027560">
        <property type="entry name" value="PEFG-CTERM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04296">
        <property type="entry name" value="PEFG-CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH33783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="285" mass="30493" checksum="AABB24BC0B6F14B2" modified="2018-07-18" version="1">MNFKRSTTSMAIALMALSLISMTSIQQDVFAQSQGMSLTAQADRDSDIIMVTGKTISKITDVTFRVTSPSGNNVVGIAQVSPDVNGEFATQFKIGPTWTENGFYTVKAMQSPQQNSLYTLKVLVEVVNGMAKKTLVTETNMESGIVYTTPNVATDKGIEIYADAVIGSTTINIEGTTDRVSQDVTLTVTAPNGNVVSVAQVSPMLNGEFTKAITTGGPLWKQDGYYTVSAKQFDDPKYTASIQVDIKDGVVVPEFGTIAAMILAVAIISIIAVSAKSRLSIMPRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQB4</accession>
    <name>A0A2S2KQB4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1059 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_06470</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876491.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQB4"/>
    <dbReference type="GeneID" id="76210004"/>
    <dbReference type="OrthoDB" id="9023at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009409">
        <property type="entry name" value="DUF1059"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06348">
        <property type="entry name" value="DUF1059"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH33856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="55" mass="6459" checksum="B72109E983558F7C" modified="2018-07-18" version="1">MAQLKCRDYGFECDFVADGEMEQVIENFRTHTDDEHGIDYSKEAVMQFLLRKQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQH6</accession>
    <name>A0A2S2KQH6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerase subunit Rpo5</fullName>
            <ecNumber evidence="2">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">DNA-directed RNA polymerase subunit H</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpo5</name>
        <name evidence="2" type="synonym">rpoH</name>
        <name evidence="4" type="ORF">NZNM25_05720</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal Rpo5/eukaryotic RPB5 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="2"/>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048117695.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQH6"/>
    <dbReference type="GeneID" id="24821062"/>
    <dbReference type="OrthoDB" id="30537at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.20">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00025">
        <property type="entry name" value="RNApol_Rpo5_RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014381">
        <property type="entry name" value="Arch_Rpo5/euc_Rpb5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000783">
        <property type="entry name" value="RNA_pol_subH/Rpb5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035913">
        <property type="entry name" value="RPB5-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007129">
        <property type="entry name" value="PRK09570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01191">
        <property type="entry name" value="RNA_pol_Rpb5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55287">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNA polymerase subunit H/Rpb5 C-terminal" evidence="3">
        <location>
            <begin position="10"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH33781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="83" mass="9411" checksum="388168185175B6B6" modified="2018-07-18" version="1">MATKKNQVLVPDHIYVPKHEIISKQEAEEVLKKYNCKPTELPLIFVNDPAILGLGIKPGDMIKITRKSPTAGESLYYRYVVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQM4</accession>
    <name>A0A2S2KQM4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase M50 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">NZNM25_06220</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NM25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876471.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQM4"/>
    <dbReference type="GeneID" id="76209977"/>
    <dbReference type="OrthoDB" id="19110at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06160">
        <property type="entry name" value="S2P-M50_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044838">
        <property type="entry name" value="EGY1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31412">
        <property type="entry name" value="ZINC METALLOPROTEASE EGY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31412:SF0">
        <property type="entry name" value="ZINC METALLOPROTEASE EGY1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="155"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="254"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="298"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="343"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M50" evidence="9">
        <location>
            <begin position="126"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GBH33831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="368" mass="40902" checksum="16C72E164CF80222" modified="2018-07-18" version="1">MEDNSQEDIISLVKSIFEVSDFTKTEFSLEFRIEDFDFKSKFENLARKLENMNYAGKLEEIDNGRYVIIQKFSPKKQRKWMSSSWTPRILFAIVISFVMIDGYYRTSGTNSIIEIGDPLEMAGVYTLSLLGILGIHELGHIIAAKIHRLKTTWPYFIPGLPVIGIPTFGAFIQSRGLTINREILFDVAIAGPIAGLVIAIIVSIYGAYTAPMLDQDIAAGLFAESKLIEWNQGEPLLMTASLALFGKGGDGQEVIMTPVMFAAWIGFLITFLNLLPAWQLDGGHMARTLLGPKLHRYATFGSMAILILLNYWLMAMLILIMSSRNPGATPLDDISPLSRNRKLAYIGIIGLAILCAPLPSDFLPNFLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQN4</accession>
    <name>A0A2S2KQN4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">4Fe-4S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">NZNM25_06320</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000002">
        <property type="protein sequence ID" value="GBH33841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876480.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQN4"/>
    <dbReference type="GeneID" id="76209989"/>
    <dbReference type="OrthoDB" id="23833at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3270:FF:000013">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit I protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010226">
        <property type="entry name" value="NADH_quinone_OxRdtase_chainI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849:SF35">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="54"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="96"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GBH33841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="165" mass="18078" checksum="87C8335C8AC6D865" modified="2018-07-18" version="1">MGTATGIIRALNSGIKHIAVKRFTLRYPEEKLKFVGDGYQFDPSTGVGIAGLKGRHMLFHDHCTGCQLCSIACEGVAEAIAMVKVPEEQKQNKKSIMPQIDYGKCVFCGLCVDACPFYALYMTNDYELSSFSKEGLIYTPAQLAVKPYVAQDSEIQITDRGATHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KQV9</accession>
    <name>A0A2S2KQV9_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_08430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876690.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KQV9"/>
    <dbReference type="GeneID" id="76208828"/>
    <dbReference type="OrthoDB" id="6229at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="98" mass="11445" checksum="477927870D6DE86F" modified="2018-07-18" version="1">MAIPLRNTIYDKIKEANSLTDIELYKSLTKDGHIIPEDKFNKLLLDLEILGLITVAWITKEERRIEAKVVEEEVDSIEEQNKEMMEKDYEASFPGFEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KRE6</accession>
    <name>A0A2S2KRE6_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CAAX prenyl protease 2/Lysostaphin resistance protein A-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_10100</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000004">
        <property type="protein sequence ID" value="GBH34219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109876831.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KRE6"/>
    <dbReference type="GeneID" id="76208657"/>
    <dbReference type="OrthoDB" id="8232at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080120">
        <property type="term" value="P:CAAX-box protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="2">
        <location>
            <begin position="59"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="222" mass="25256" checksum="E67BA5AA61ABE240" modified="2018-07-18" version="1">MIREIIDINPKTWLKPFQKTSIFYLLKMGLFYHGLGVILMYAGSFFATNIISDYEIPQFPVSITLAVSSGLLEESIFFGMPYYMTGNPLILLGSGIIWSASHLFSSSVFSVEALAYGGFLLTIPHIFFSIRTWISKKGWFAIVFHSAWNFSFLILYCMLGLRQCSIVNDMYDVINLIMAISAGVIVYLAYQNKKRYLNRFLYLIPVGIIFVSSAILFSDYVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSC4</accession>
    <name>A0A2S2KSC4_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">NZNM25_12420</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutaredoxin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioredoxin family. DsbA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877085.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSC4"/>
    <dbReference type="GeneID" id="76209262"/>
    <dbReference type="OrthoDB" id="15256at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13887:SF14">
        <property type="entry name" value="DISULFIDE BOND FORMATION PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13887">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13462">
        <property type="entry name" value="Thioredoxin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="24"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="213" mass="24606" checksum="6CF6B7DBEF97EB11" modified="2018-07-18" version="1">MTKIYLLTIPVIIGIVTGMSMVYFPENQDSKLLTVQKLIHNGSPIMGDPNAPITILEWGDYQCTFCYKFHQNTLDIIYEEYIKTGKVKLVFKDFPLNGSDSFLAAEAAYCADDQEKYWEYHDELYNNWGGERTGWITRESLDKFAQTVNLDLDEFNKCLNEKKYQNKVITLHEFGKEVGIDATPSFLVFNDNKIIKIRGNQPLEVFLKTFAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSE2</accession>
    <name>A0A2S2KSE2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CoA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_13910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877204.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSE2"/>
    <dbReference type="GeneID" id="76209108"/>
    <dbReference type="OrthoDB" id="42776at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33303:SF2">
        <property type="entry name" value="COA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33303">
        <property type="entry name" value="CYTOPLASMIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA-binding" evidence="1">
        <location>
            <begin position="14"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="137" mass="15653" checksum="3CAE59E927EECFA3" modified="2018-07-18" version="1">MEQDKHTDEQIHNFLSMRRVAVIGMSKDSSKAAHYVPKYLLENGYDITPVNPTSVEILGKKCYDSVSKIDGDIDIVDIFRPSDQVLPVIQDAIKKKPKVIWLQEGIHNSEAENMARKEGIDVVFNRCMLAEHQRLMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSH3</accession>
    <name>A0A2S2KSH3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_11960</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829078.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSH3"/>
    <dbReference type="GeneID" id="76209310"/>
    <dbReference type="OrthoDB" id="4877at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="48" mass="5518" checksum="E689D24A1AA9D2E7" modified="2018-07-18" version="1">MTEQDFVEKMNPSPTFWNDATKYASISKDEAFVEEVAYMMVTLHRTGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSJ7</accession>
    <name>A0A2S2KSJ7_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sporulation kinase C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">kinC_1</name>
        <name evidence="9" type="ORF">NZNM25_14330</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877239.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSJ7"/>
    <dbReference type="GeneID" id="76209062"/>
    <dbReference type="OrthoDB" id="8127at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00075">
        <property type="entry name" value="HATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065:SF10">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065">
        <property type="entry name" value="SENSOR HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="PAS" evidence="8">
        <location>
            <begin position="13"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="152"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GBH34642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="374" mass="42206" checksum="960B11CB856B2558" modified="2018-07-18" version="1">MPQKINSFNDANNLDIYRELYENSLILFRTINLKGEIINCNKAYSQAIGYSKNEIIGQNIFAHTAIESLDEMTEMFNTWKSGKCYSNKEVWLRKRNGEKFLGLFSANNLYDNGVLVGSNTAIQDITEITLLKQEFKHIKRKRQEIMGELSVRIAHDLKNPLSVIKNSIEILQTKNKSLNEQHLNLFQAINRSILRMDHQINQVFDFVNPKPLDLEECSIGKIIESVISEIIDTANVIVQLPENDCMIKCDKTKMENVFANLLLNAIQAMEGNGTLAITLNESDSHTKIRIKDSGPGIPDELISKIFDPLFTTRQIGTGLGLSSCKTIIEQHGGSVSFESKIGQGTVFEIELPKSKLSLTTNNANFVLESEKLYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSN1</accession>
    <name>A0A2S2KSN1_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">lsrF</name>
        <name evidence="3" type="ORF">NZNM25_14810</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeoC/FbaB aldolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877279.1">
        <property type="nucleotide sequence ID" value="NZ_BGKI01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSN1"/>
    <dbReference type="OrthoDB" id="6329at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00958">
        <property type="entry name" value="DhnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050456">
        <property type="entry name" value="DeoC/FbaB_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041720">
        <property type="entry name" value="FbaB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006081">
        <property type="entry name" value="PRK08227.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47916:SF1">
        <property type="entry name" value="3-HYDROXY-5-PHOSPHONOOXYPENTANE-2,4-DIONE THIOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47916">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE CLASS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01791">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038992">
        <property type="entry name" value="Aldolase_Ia"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="261"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="266"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="303" mass="33222" checksum="D476480945D531A3" modified="2018-07-18" version="1">MDWGLKNRISGIIKPQNNRALMLAVDHGYFLGPTEKLEDPKKVIAPLLKHCDSLMLTRGVQRACVPATTNTPMVLRVSGGSSIIGEDLSQEDITVSIEDAIRLNASALAMSIFVGSKYEYQTIANLGKLVNEAAKYGLPVLAVTAVGKELGKDARYLSLACRVAAEQGAHIVKTYYCDNFEKVVQSCPVPIIVAGGKKIPERDALQLTYNAIKGGAVGVDMGRNIWQSEHPVSMIKAVRAIVHGNQNVDQAFKLYKKLVSEEPKQKSNTQKKPFNPNQKKSNTQKKPFNPNQKKSNPPNQTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSP3</accession>
    <name>A0A2S2KSP3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Extracellular protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_14540</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSP3"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="24012" checksum="218E5BE8B946E017" modified="2018-07-18" version="1">MILMKKIVLISAIAGFVIVGLVSSLLLSPNSLDAKTSMPVYEIDFTFYDVEKIQKSLNEHNVFMSTPSAITDHTIDQYCTFFDSTNTKRTVEYCTTTALLNSDGKTIGNLNMGGTTYNPIMALAIVDASPFLNSKADEVDIIFQTMIETLVCTCWEDRQPGGFESVSEWIDVAQEKYAESSQTTIKSEINGLDNKRLILEITSTGKSYLWTLIVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSU8</accession>
    <name>A0A2S2KSU8_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_14220</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877229.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSU8"/>
    <dbReference type="GeneID" id="76209073"/>
    <dbReference type="OrthoDB" id="115601at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047558">
        <property type="entry name" value="TPR_END_plus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF8">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13174">
        <property type="entry name" value="TPR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48439">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="44"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="78"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="112"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="146"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="180"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH34631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="273" mass="31012" checksum="914CC6F4A0988492" modified="2018-07-18" version="1">MGLFGGKENSEDLMYNAMSLIEKNQPKGAISLFNKVIKQEPENTSAIFNKGLALNQIKKYSDAITCFDKLLEINPKDAQAYNNKGIAMAEMDNIQDAAECYDKAIEADPKHAASYFNKGVLLDKLKEHEEALQVLDKAITIEPRKPNALFYKGIILGKIKRHEEALNCFDKVYKNNPSHMDAFFHKGIELAELGKHENAIEIFDKILSKHKDNVNIIYAKSRSKAELGMDPEALKLLKQAIAKNPKIIRSWAKEEKAFEKFHTNDEFRKIVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KSX2</accession>
    <name>A0A2S2KSX2_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GRAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_15250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877363.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KSX2"/>
    <dbReference type="GeneID" id="76208463"/>
    <dbReference type="OrthoDB" id="9104at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="12537" checksum="C0E4B36F8F54ADB9" modified="2018-07-18" version="1">MEKGENRIKQEDCSEDNLGAGILTLTNKRLAFDKTNARIMDFSKHFGETVMDIQLNDITKVWKEGLLMKKVCFTAKTKEGEQTFKFGVFNTKDWTKSIQRQIDENKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT03</accession>
    <name>A0A2S2KT03_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Adenosylhomocysteinase</fullName>
            <ecNumber evidence="6">3.13.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">NZNM25_13790</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="8">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.13.2.1" evidence="6"/>
    <dbReference type="EMBL" id="BGKI01000007">
        <property type="protein sequence ID" value="GBH34588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT03"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004013">
        <property type="term" value="F:adenosylhomocysteinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033353">
        <property type="term" value="P:S-adenosylmethionine cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00401">
        <property type="entry name" value="SAHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000004">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00936">
        <property type="entry name" value="ahcY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004005">
        <property type="entry name" value="PRK05476.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420:SF0">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001109">
        <property type="entry name" value="Ad_hcy_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S-adenosyl-L-homocysteine hydrolase NAD binding" evidence="10">
        <location>
            <begin position="160"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="191"/>
            <end position="196"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="NCBIfam" id="TIGR00936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001109-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="389" mass="43062" checksum="DB1777802663CD8B" modified="2018-07-18" version="1">MQILDNTINRFKKSKPLKGVTLGFCLHITKETSVLLMGAKELGATVACCGGNPLTTQDDIAAFLASQGIHVYAWHGQSVKEYDWCIDQVLKHKPTILTDDGADMNIKAHFDKRFNTMKILGATEETTAGVTRIRAVENQGKLRYPVILVNEAYTKHMFDNRYGTGQSTIDGYLRAMNLLMASKRVVVIGYGWVGRGVASRFQGMGSKVIVTEIDPVKALEAHMDGFEVMPMSQAAKIGDMFVTCTGMTSVIRKEHILQMKNGAIMGNVGHFDVEIDSKFLLKQSKSVKEVRPNLDECTLKNGKVVYLIGQGRLANLVAAEGHPPEVMAQSFSNQILSVLYILKNHNKMENKIINVPEEIDKQVAIDALAAMNVKIDKLTPEQVKYANSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT27</accession>
    <name>A0A2S2KT27_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_15870</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877424.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT27"/>
    <dbReference type="GeneID" id="76208400"/>
    <dbReference type="OrthoDB" id="6601at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="127" mass="14448" checksum="16F7924CB75250FB" modified="2018-07-18" version="1">MEISIEPWKKLIIHEVIEYKFDDWVKQIAFSTRSSGGGIPTMQWTNGIVFSPANFPTTNVTAEEQLRGILHWSSVSFAIKEKFEKQIVKENATINLVDVSVNEIFKELAASLKKQSKYVNLESGKDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT45</accession>
    <name>A0A2S2KT45_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">THIF-type NAD/FAD binding fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_15420</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HesA/MoeB/ThiF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877377.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT45"/>
    <dbReference type="GeneID" id="76208446"/>
    <dbReference type="OrthoDB" id="7915at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00757">
        <property type="entry name" value="ThiF_MoeB_HesA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000080">
        <property type="entry name" value="Thiazole biosynthesis adenylyltransferase ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF102">
        <property type="entry name" value="ADENYLYLTRANSFERASE AND SULFURTRANSFERASE MOCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="2">
        <location>
            <begin position="104"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="443" mass="47886" checksum="45F586B2988FF73C" modified="2018-07-18" version="1">MANITFTIPSVLNQSGGEKKTEISADSLTDAFAKISELMGDDFKRRVLESDGVPRSLINIYINGKNAKFSSGMDTLLKDGDEVYILPAVAGGSEELSAKELDKFSRQVMLEEIGYNGQLKLKNSKVCVVGAGGLGNPITSRLAAMGVGTLRIVDRDVIELSNLHRQTMFDEDDVGQIKVEVAAKKLQKLNPDCNIEALAVSVNDYTALEVVEGCDVVIDALDSVNARYALNKACVKFGIPFVTGAAVGVSGQAFTILPKESACYYCMFPDLNEDTMPTCSIEGVHPSILSIVGGIEVAEAVKIIIGKKPSLSEKILHIDLENLDFNSVRTFRAEECPICGTGKLEVIPKEELILEELCGRNRGKRTYSITPTETFDLDVNNVTDIAKEKGFIVDNQGNLGLSMRTNELSVSFMKKGSAVIVGPKDEAEAIALYKSLLGKEIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT53</accession>
    <name>A0A2S2KT53_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_16130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_200829100.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT53"/>
    <dbReference type="GeneID" id="76208373"/>
    <dbReference type="OrthoDB" id="3141at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="49" mass="5678" checksum="1D6351C603E6E9C5" modified="2018-07-18" version="1">MSTLIQYKYDKNADQSLSLEEDEIKNPVSANITAMDMIGLTWILQDDEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KT88</accession>
    <name>A0A2S2KT88_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aspartate carbamoyltransferase</fullName>
            <ecNumber evidence="7">2.1.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Aspartate transcarbamylase</fullName>
            <shortName evidence="7">ATCase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pyrB</name>
        <name evidence="11" type="ORF">NZNM25_16530</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NM25</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 7">Catalyzes the condensation of carbamoyl phosphate and aspartate to form carbamoyl aspartate and inorganic phosphate, the committed step in the de novo pyrimidine nucleotide biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterooligomer of catalytic and regulatory chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="7"/>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877479.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KT88"/>
    <dbReference type="GeneID" id="76208332"/>
    <dbReference type="OrthoDB" id="7792at2157"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00116"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004070">
        <property type="term" value="F:aspartate carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000021">
        <property type="entry name" value="Aspartate carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00001">
        <property type="entry name" value="Asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002082">
        <property type="entry name" value="Asp_carbamoyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00670">
        <property type="entry name" value="asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002032">
        <property type="entry name" value="PRK00856.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753:SF6">
        <property type="entry name" value="ASPARTATE CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753">
        <property type="entry name" value="ORNITHINE CARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00101">
        <property type="entry name" value="ATCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase carbamoyl-P binding" evidence="10">
        <location>
            <begin position="7"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase Asp/Orn-binding" evidence="9">
        <location>
            <begin position="154"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GBH34862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="309" mass="34795" checksum="2E06413346773C67" modified="2018-07-18" version="1">MNQFYQKDIISIKDFDKNSLETIFSATDKIMQLDPSDRREICKGKTLGYLFYEPSTRTRLSFDAAMASVGGNSLGISNMSSSSTQKGESLADTVRVMSIYSDVLVLRHTLDGSSRFAAEVSEKPVINAGSGTEEHPTQAIQDLFTIKKEKRKIDGLKIGIVGDLKYGRTVYSLLYGLGNYDVDVHLISPESLKIRSDSVYEIKKRLDFAESTDITETIDEFDVLYVTRIQKERFPDEEEYLKVKGSYVVGLDLIKQMKDDAIILHPLPRIDEISADVDKTKNAKYFEQAEYGKYTRAAILGLILNENGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTK3</accession>
    <name>A0A2S2KTK3_9ARCH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1059 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_17910</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877595.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTK3"/>
    <dbReference type="GeneID" id="76210330"/>
    <dbReference type="OrthoDB" id="9023at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH35000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="63" mass="7417" checksum="CBC32070B4A52CC9" modified="2018-07-18" version="1">MTLKLRCNDYGFECEFILDEEKTVGLIEKLRNHFEEEHGIDYTIEAVTQMITNRGHSLESIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTK4</accession>
    <name>A0A2S2KTK4_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_15860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000009">
        <property type="protein sequence ID" value="GBH34795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877413.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTK4"/>
    <dbReference type="GeneID" id="76208402"/>
    <dbReference type="OrthoDB" id="2682at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="158" mass="18174" checksum="BF440F039A7D7C2D" modified="2018-07-18" version="1">MAVKKKATAKKKPVKKVDSKAKKQVKSASSKKPEFEKAWKEYNAALNGWKESLAQWQKATNETLMTYHDACQKALESDAELLKKVSSSWENTWEEIGPQYIKQQTKMIESIFKETNIESIKKFNEQWEKFLSTSGDDSIAAYQEAIKKFNQAWQSGQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTN0</accession>
    <name>A0A2S2KTN0_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">NZNM25_17080</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTN0"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="55"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GBH34917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="117" mass="13254" checksum="B13DD084A4DABC7C" modified="2018-07-18" version="1">MAVPITWFLTFTCWISQKSTDYMHKHASSHAQTEKKSLSSSQTIQTTTSGNQVTLSEIKQIQSELSTELNNLKESVKLKDGEIERLNQEISNLQTLVQIESLKTELANLKMLASKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTN2</accession>
    <name>A0A2S2KTN2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_17940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH35003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877597.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTN2"/>
    <dbReference type="GeneID" id="76210327"/>
    <dbReference type="OrthoDB" id="10815at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH35003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="90" mass="10301" checksum="12966AFCD9288822" modified="2018-07-18" version="1">MAIPADVEEYVEKHIKLMISQTETYLPFIKVAFPYSNNVADGVYSLIIGSALSVFVNQFAMKMKYPTAEDFTDFGKIAIKYRDQVDKFFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTR2</accession>
    <name>A0A2S2KTR2_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Phosphoglucosamine mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">NZNM25_18510</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NM25</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877676.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTR2"/>
    <dbReference type="GeneID" id="76210076"/>
    <dbReference type="OrthoDB" id="10363at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008966">
        <property type="term" value="F:phosphoglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004615">
        <property type="term" value="F:phosphomannomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03087">
        <property type="entry name" value="PGM_like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000001">
        <property type="entry name" value="Phosphoglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.50:FF:000009">
        <property type="entry name" value="Probable phosphoglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024086">
        <property type="entry name" value="GlmM_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050060">
        <property type="entry name" value="Phosphoglucosamine_mutase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03990">
        <property type="entry name" value="Arch_GlmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42946:SF1">
        <property type="entry name" value="PHOSPHOGLUCOMUTASE (ALPHA-D-GLUCOSE-1,6-BISPHOSPHATE-DEPENDENT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42946">
        <property type="entry name" value="PHOSPHOHEXOSE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="9">
        <location>
            <begin position="4"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="10">
        <location>
            <begin position="151"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="11">
        <location>
            <begin position="256"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase C-terminal" evidence="8">
        <location>
            <begin position="404"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GBH35060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="449" mass="48965" checksum="027033CD471E8B9C" modified="2018-07-18" version="1">MAKFFGTNGIRGVFSEDFTLEFVHDMTLAIGTYFECGPVLIGYDGRESSPIVCKVVSAALNSIGIDCNVAGIIPTPCLEFAVKTLGYSGGIMITASHNPPQYNGIKPAAKDGVEISREDELIIEDIYMNKNWITNPSKWGTTGIENRTIDTYIDGIFSHIDSTLIESKKFRVVLDLGNGAQAISAPTFCKKLQCETFLVNEYIDGNFPGRGSEPTPENLSLLSQTVIENNADVGIAFDGDGDRSIFCDNKGNILTGDKSALLLTQHILKKNPNSLVVTCLNSGSNIEKIAEKFNSKVIRTKVGSVEVSRKMVPINALIGFEENGGFMYGKHNQVRDGCMTLGLMLDLLATSGKTLSDEISLLPPSFTTKDKVPCPSEKVSGLISSLKEEFPNSDLTDGIKITIDPKNWVMIRPSGTEPIVRIYAEAESQEKLDSLMSEYLKKVKTTISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KTS3</accession>
    <name>A0A2S2KTS3_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">NZNM25_16560</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_263970085.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KTS3"/>
    <dbReference type="GeneID" id="76208329"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GBH34865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="43" mass="5032" checksum="00F87BA0F50A0D17" modified="2018-07-18" version="1">MAKEEIKKLLENSKKELDADYDNFVTSVKDDLNSFKKKALDRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU40</accession>
    <name>A0A2S2KU40_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Bifunctional phosphoglucose/phosphomannose isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">NZNM25_19400</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NM25</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PGI/PMI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000012">
        <property type="protein sequence ID" value="GBH35149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_306307579.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU40"/>
    <dbReference type="GeneID" id="76210170"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004476">
        <property type="term" value="F:mannose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05017">
        <property type="entry name" value="SIS_PGI_PMI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05637">
        <property type="entry name" value="SIS_PGI_PMI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019490">
        <property type="entry name" value="Glu6P/Mann6P_isomerase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035484">
        <property type="entry name" value="SIS_PGI/PMI_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10432">
        <property type="entry name" value="bact-PGI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SIS" evidence="3">
        <location>
            <begin position="35"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GBH35149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="337" mass="38272" checksum="A92FCDA458EC0FBE" modified="2018-07-18" version="1">MIKLEDLRKIDVKGMYKIYDEWPKIARESYFSNLSEINFERCSHIVFAGMGGSGAIGDIFSAILSKTNTHVTVVKGYHLPRTVDSKSVVVITSISGNTVETLAMLDDARRIGSKIIAFSDGGRIYEICLKNNIEHRNVSKYHSPRASFTSFLYSMLRVLKPIIPIEESDILESIENLEKISKTINSENISMSNPAIQISEWINNSPIIYFPLGLQPVAIRYKNSLQENSKMHAMIEDIIESSHNGIVGWGTKNNFQPILLRGQDDYTTTKERWEILKEFFESQNIEYKEVISENGNILTKIICLIYLLDYSSIFLAVKLGVDPTPVVPINFVKERLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-07-18" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2S2KU70</accession>
    <name>A0A2S2KU70_9ARCH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">NZNM25_17890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Nitrosopumilus zosterae</name>
        <dbReference type="NCBI Taxonomy" id="718286"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Nitrososphaerota</taxon>
            <taxon>Nitrososphaeria</taxon>
            <taxon>Nitrosopumilales</taxon>
            <taxon>Nitrosopumilaceae</taxon>
            <taxon>Nitrosopumilus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2018-05" db="EMBL/GenBank/DDBJ databases">
            <title>genome sequencing of Nitrosopumilus sp. NM25.</title>
            <authorList>
                <person name="Mori K."/>
                <person name="Nakagawa T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NM25</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BGKI01000010">
        <property type="protein sequence ID" value="GBH34998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_109877593.1">
        <property type="nucleotide sequence ID" value="NZ_AP026695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2S2KU70"/>
    <dbReference type="GeneID" id="76210332"/>
    <dbReference type="OrthoDB" id="3351at2157"/>
    <dbReference type="Proteomes" id="UP000245829">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GBH34998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000245829"/>
        </source>
    </evidence>
    <sequence length="143" mass="16435" checksum="8BC2E7E097002038" modified="2018-07-18" version="1">MKSGLIFSLTLIILTSIPFVFAINTEQEPEPPEIPEPSPEPEPIRMPEPAPAPAPFPEETESEKIKRLTEENNKLKQENTNLRGDISYLKNENMRLQDMISELNETITSLKEITMEQVRVIIDLANRLKDVLFEKIFYPTINL</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>